<pre>LOCUS       KJ755556               12044 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain H 311a serotype O95:K+:H33 O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ755556
VERSION     KJ755556.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12044)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12044)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Baranzoni,G.M.,
            Liu,Y. and Debroy,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="H 311a"
                     /serotype="O95:K+:H33"
                     /db_xref="taxon:562"
     misc_feature    1..12044
                     /note="O-antigen gene cluster"
     gene            117..1205
                     /gene="rmlB"
     CDS             117..1205
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="AIG62515.1"
                     /translation="MKILVTGGAGFIGSAVVRYIINNTEDSVVNVDKLTYAGNLESLA
                     KIANHPRYAFERADICDASVLARIFAEHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARQYWSHLDEARKSAFRFHHISTDEVYGDLPHPDELPAGSTLPLFTETTP
                     YSPSSPYSSSKASSDHLVRAWRRTYGLPTLVTNCSNNYGPYHFPEKLIPLIILNALAG
                     KPLPVYGNGEQIRDWLFVEDHARALYKVVTEGTAGETYNIGGHNERKNIEVVKTICTI
                     LDEIIADKPAGISHFAELITYVTDRPGHDLRYAIDASKIKNDLGWVPQETFESGIRKT
                     VEWYLNNESWWKRVMDGSYAGERLGLAE"
     gene            1220..2086
                     /gene="rmlA"
     CDS             1220..2086
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase 2"
                     /protein_id="AIG62516.1"
                     /translation="MKGIVLAGGSGTRLYPITQGVSKQLLPVYDKPMIYYPISVLMLA
                     GIRDILIITTPDDIDAFKRLLGDGSQFGINLSFAVQPSPDGLAQAFIIGEEFINGEPC
                     ALVLGDNVFFGQSFSKKLEAAFQLTSGAMVFGYQVQDPERFGVVEFDSEFKALSIEEK
                     PAKPKSNWAVTGLYFYDKNVVEMAKQIKPSVRGELEITTLNQMYLERGGLQVELLGRG
                     FAWLDTGTHDSLIEASQFIHTIEKRQGLKVACLEEIAFRKGWMNKEQLSAQAKKFSKN
                     DYGRYLQMLISE"
     CDS             2130..2459
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AIG62517.1"
                     /translation="MEYSNLDSMVRGWFVGGFEPTLYKTTDVEVAVQHFTAGDHEAAH
                     CHKIATEITVIISGRAKMAGRIVVAGDIVKISPGEYTDFEALEDTVTAVVKLPGALND
                     KYLKGDE"
     gene            2459..3214
                     /gene="glmU"
     CDS             2459..3214
                     /gene="glmU"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein"
                     /protein_id="AIG62518.1"
                     /translation="MLNIVVPMAGRGSRFAKEGYVDPKPLIKLKDKRMIQVVINNLTP
                     SIPHRFIFICQRQHVIDYSLDRYLKEWAPNSEVICIDGITEGAACTVMCAEELINNDE
                     PLMIANSDQWIDIDINDYLNYMKHEDLDGLIMTMKADDPKWSYAEMNENNHVTRVVEK
                     EVVSDEATVGIYNYKKGADFCRSVKRMIALDQRSNGEFYVAPAYTLMYENEGAKIGIY
                     NIGEEANGMYGLGIPADLELFLSLDVSRKALDF"
     gene            3217..4863
                     /gene="gtf1"
     CDS             3217..4863
                     /gene="gtf1"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="AIG62519.1"
                     /translation="MHVAKKICLIGHECGSYKGQGGIATYIELTAKGFAKLGLEVHVI
                     YIHGSGVDCDDIKSWKIKNDPSLYKISLEVDKVLEEIKPDFVECTDFIGMMSYTLSKR
                     AIRGLDYKCIFITNHHTGIKEVWEWGTQLKFNETAKSWMKSLYIAERTQSLLSDANFS
                     TSNFLASYLDANHIESYQVSPSYYEMNDNFGETNKNHYDSNLLRIISLGRFELRKKQE
                     LLIKATCELLDEGYDIETTLIGNSGGDLYTHQDYMEYCYSLIPTEYKHKFHFYDFMPY
                     KLLQKKYTEYDLFVIPSPYENFPNTALEAINYGVTVVGSKSSGVADMIGEDLSDYCFL
                     PDSVSDIKRVILKYNQLDAEERATLRLKQRLSLKNLTCFEKSIQERFEKYQAVDELRS
                     EKNIDDKDILIVYQDKNGLINKVEYCSEIYYSLGSKWKDFIREIKYIVLTSEEYQFNK
                     IENYYPAPGIISCFSSYDPYGTVREIQQAEKGYSSLTLCVETIPYNEGDSISEYIAQV
                     LLSTSDVIFFIDEEKKVEQGISIDIKYAIDKIKFNYSGEK"
     gene            4860..5876
                     /gene="wavE"
     CDS             4860..5876
                     /gene="wavE"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide synthesis"
                     /protein_id="AIG62520.1"
                     /translation="MNIIIHGPLEKDFLFKNLNKIRHASEDDHIIISVYNEELEKTKV
                     IIDSVPVRNKVTLIGSDDVFNPGFFNINRQINLVSAALNVIEDESDFILKVRMDQTIN
                     FRWVRKVVTEKHEELKDKLITTNCYTRKDRLYHPSDMFLAGTKKTLRAYYPEEFFQET
                     HMDNLLKIKELVLSGYKGGYHKYWPESRLFMNYLSILGEELQDTEICSQSQLKKHVYI
                     FNSWDIGLKWKKFLKGKVNVLPYFFVLRPFERGPLENAENFLASDLTGYTKKPLREMI
                     YNSLAKLYFKSGAYLANPIFIDNKSLIPKLLRKMFDHSYKFIPPILHNLMYKVAKRVY
                     YAFK"
     gene            5863..6801
                     /gene="fcf1"
     CDS             5863..6801
                     /gene="fcf1"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydro-6-deoxyglucose reductase"
                     /protein_id="AIG62521.1"
                     /translation="MHSNKIYIIGWSGFIGRQYIHYLDKTGFKGEVFLNSRADDEQKY
                     TSSTDNITIKYVNYEEMITGLNCSRSTVLYLANMYSPGESNIYPLNSVKENIIPFITF
                     LEDIKNNASNIRFIFASSGGTIYGDSQEGECCSENHLLAPKTIYAANKIAQENYLNVY
                     HVNYGLDYRIARIANPYGPGQILKGGQGLIPAILRSSLNNESLPVSGDGNGTRDYIYI
                     DDLCSLFFSLSSYSGACRTFNAGSGKEYSIMEIISCFNAVHDRPIKYHHVKTESSVVS
                     RIVLDISRAKNELGWSPKTSLSDGIRAFINWSIQVM"
     gene            6831..7889
                     /gene="wavE"
     CDS             6831..7889
                     /gene="wavE"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide synthesis"
                     /protein_id="AIG62522.1"
                     /translation="MTYTTQISAQDVSIVIQGAIFDTKKQKVDAQFIEYLEKVISIFI
                     GCEFIVSTWEFPREIYFGLCEKYPQVNFVLNADVGPIIKIVDDVPIVTNVNRMIFSTI
                     SGLREVNRKYAIKLRTDSFLYNDSILQSLYYVMNKREFFGLDLEKRNERYRIFDHHVI
                     NCNLFARNPRSHLPFLYHPGDIFLAGLTKDLIKLFNIPLATEDLIKKCNSVRNTCFMK
                     LVPEQYIWVQCIKMSRSTDTFDYSNFTYDEKEIEKSEQYYLNNFVPYTAEELGFCWPK
                     HREVYFNKGSSSIYDHEDWHHLYHKYIDGFEQPTSYAHKKRSVVIFFMKMYFFIRSSL
                     LKIKFIRKFAYKVFVKRG"
     gene            7893..8711
                     /gene="wzm"
     CDS             7893..8711
                     /gene="wzm"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ABC transporter permease"
                     /protein_id="AIG62523.1"
                     /translation="MKGFVSTPSSTTRFSEVFESRNLIAELIRRDFNVRYKQTGLGVL
                     WAIINPGVNILLYLFVFGLLVRLPTPEYNAPYAAVLISAILFWNLFSTSMLTASDALI
                     NNMHLVTKVYFPRLSLCIASVFVALIDFLIAFIVFVPLALLYHVDISLIRLLLLIPCI
                     IITLMLGCGFGCAMAILKLKYRDIRHILPLITQVIFFASPIVYTLSIVPEQYKIFYSF
                     NPITGIVMMSRWAILGGAPIDLTMLTYSLISAFVVMCLGVIYFVKNDRSVADYE"
     gene            8708..9439
                     /gene="wzt"
     CDS             8708..9439
                     /gene="wzt"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen export system, ATP-binding protein"
                     /protein_id="AIG62524.1"
                     /translation="MIEFDSVSKQYEINHRRAKSLRDVFSFNKKPDANAEIFKAVDDV
                     SFSIDSGKSVGVLGRNGAGKSTLLKLLTRIISPTSGKITVDGSISCLLEVGAGFHHEL
                     SGYENIFLCGSILGMSRKQVKSKIESIIDFSEVEKFIDEPVKYYSSGMYMRLAFAIGA
                     HLDSDILVIDEALAVGDSRFQNKCLNKIDEIRKSGKTIFFVSHSIEQVKKVCDTSIVM
                     DHGKLLFQGNTDEAERIYNSLTNGA"
     CDS             9442..10077
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="AIG62525.1"
                     /translation="MIIISHRGYWKLSSEKNTALAFKRSFSLGYGTETDIRDYKGSLV
                     ISHDIPDESSLSIDSFFEIYNQYDVKGPLALNVKSDGLQKLIKEKLNEYKVSNYFFFD
                     MSIPDTLGYLSEKLNTFVRVSEYETLNDLYEDADGVWIDGFKENIITESLLEKIISDG
                     KRACIVSADLHKNDPATQWKLLKSFKTNILQSSNIILCTDLPEKASEFFYG"
     CDS             10070..10714
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="AIG62526.1"
                     /translation="MDNKIKAVIFDMDGVLIEAKDWHYEALNRALGLFGLEITKHEHL
                     TTYDGLPTKDKLVMLSRDKGLSNALHTFINEMKQQYTMDMVHKFCKPMFHHEFALSML
                     KQDGYKLAVASNSIRNSVEVMLDKAKLLAYLEFFISNQDVKKGKPDPEMYTLAISKLG
                     LRPSECLIVEDNENGIKAAKASGAHVLEVETVYDVNYQNIKQRIKKIENDEGGK"
     CDS             10711..11778
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="AIG62527.1"
                     /translation="MINILIPMSGENLYETSSNFIYPKILTEVANKTLLEYSQLIFDN
                     LRDEKNLIYIAPEDKLNTLGLKSIIQTITDGKGKIVALQGQTKGAVCSCLMAIDDLSL
                     DDELIISSADHYIDDNLQRIVNDFRVMDADAGVLTFESVHPKWSFVTLNEHGAVVQAA
                     EKKAISRTAVAGLYYFKKARDFVEAAMNLIRKDGDINGNFYLSSCLNELVLLGKVIKC
                     RPLQDAIYHNFYDAHAVKSFELAQTSLSNSVKQLTNSYIEAFNSKDIEECLFLFSDDA
                     VLVEPNKTFTGKDEIKELLNDIFVNNSKLQFRADNIIADDSKSAIKFTLELTNETVHG
                     VDFIEWNSAGKIHKLTAFLND"
ORIGIN      
        1 actggtagct gtagagccag gggcggtagc gtgttttaat ttctgctaac tacagtcctc
       61 cggctctatg aattaagttc gaatctaaac attacgacgc aatacggaat agtcttgtga
      121 aaatacttgt cactggtggt gcaggtttta ttggatctgc agtagttcgt tatattatta
      181 ataatactga agattctgtg gtaaacgtag ataagttgac ctacgctggc aacctggaat
      241 cgttggcaaa aattgccaat catccgcgct acgcatttga gcgagcagat atctgtgatg
      301 cgagcgtttt ggcacgcatt ttcgcggaac atcagcccga tgcggtaatg cacctggcgg
      361 cagaaagcca tgttgaccgt tctatcaccg gtcccgctgc gtttatagaa acgaacatcg
      421 ttggtaccta tgtattgctg gaagccgcgc gccaatattg gtctcaccta gacgaggcgc
      481 gcaaatcggc atttcgtttc catcatattt ctactgatga agtctacggc gatctgccgc
      541 atccagatga attgccagcg ggaagcacgc tcccactgtt caccgaaacg acgccttact
      601 ctccaagtag tccatattcg tcttctaaag catcaagcga tcatttagtg cgtgcctggc
      661 gtcgtaccta tggtcttccg acattggtga caaactgttc gaataactac ggtccttatc
      721 acttcccgga aaaactgatc ccactcatta ttctgaacgc cctcgcaggc aaaccgcttc
      781 cggtatacgg taacggcgag cagatacgag attggctctt tgtcgaagat catgcgcgcg
      841 cgctatacaa agtggtaact gaaggtactg cgggtgaaac ctacaatatc ggtggccaca
      901 acgagcgtaa aaacatcgag gttgtgaaga caatctgcac cattctggac gaaatcattg
      961 ccgataagcc cgcaggcatt tctcacttcg ccgaactgat tacctatgta accgaccgcc
     1021 cggggcatga cctgcgttac gccattgatg ccagcaaaat taaaaatgat ctgggatggg
     1081 taccacagga gacgtttgag tccggtatcc gtaaaacggt tgagtggtat ttaaataacg
     1141 aaagctggtg gaagcgggta atggatggct cctatgcggg cgaacgtctg ggactggcgg
     1201 aataaaaaag gaaaatatga tgaaaggtat tgtacttgct ggtggttccg gaacgcgtct
     1261 ctatcctata actcagggtg tatcaaaaca gttgttgcct gtctatgata aaccgatgat
     1321 ttattatcct atttctgtac tgatgttggc gggtatccgc gatatattaa tcattacaac
     1381 tccagatgat atcgatgcgt ttaagcgttt attaggcgat ggcagtcaat ttggcataaa
     1441 tctcagcttt gcagttcaac cttcccctga tggcctggcg caggcattca tcattggtga
     1501 agaatttatt aatggcgaac catgtgcgct cgtgttaggt gataatgtct tttttggcca
     1561 aagctttagt aaaaaacttg aagctgcatt ccaattaacc tctggagcga tggtctttgg
     1621 ttatcaagtt caagatcctg aacggtttgg tgtagttgaa tttgatagtg aattcaaagc
     1681 gttatctatt gaagagaaac cagccaaacc taaatctaat tgggctgtaa cgggtctata
     1741 tttctacgat aaaaatgttg tcgaaatggc aaaacagatt aaaccctcgg tgcggggtga
     1801 gcttgagatt acaacattaa atcagatgta tcttgagcgt ggaggattgc aagtcgagtt
     1861 gttaggtcgc ggttttgcat ggctagatac aggaactcat gacagtctga tagaagcatc
     1921 tcaattcatt catacaattg aaaagcgcca gggtttgaaa gtcgcttgtc ttgaagaaat
     1981 agctttccgt aaaggctgga tgaataaaga acaactatcg gcacaagcga aaaaattctc
     2041 aaaaaatgac tatggccgct acttacagat gctgattagt gagtaatatt acgatgtgtt
     2101 cttttttatt aaaagcaatg tgaggtaacg tggaatactc caacttagat tcgatggtac
     2161 gaggctggtt tgtcggtggc tttgagccaa ccttatataa gactactgac gttgaggttg
     2221 ccgtacaaca ttttactgcc ggagatcacg aggccgctca ttgccataaa attgctactg
     2281 aaataacagt tattatttcg ggtcgagcca aaatggcggg cagaattgtt gtagcaggtg
     2341 acatagttaa gatttcgcca ggggaatata ctgattttga agctttagaa gatacagtta
     2401 ctgctgttgt aaaattgcct ggcgctttaa atgataaata tctcaaagga gatgagtgat
     2461 gttaaatatt gttgttccaa tggctggtcg cggaagtcgt tttgccaaag aaggttatgt
     2521 ggatccaaag ccactgatta agttaaaaga caagcgaatg attcaggttg taatcaataa
     2581 tttaactcct tctattcctc atcgttttat atttatatgc cagcggcaac atgttatcga
     2641 ttatagtttg gaccggtatt taaaagagtg ggcaccgaat tcagaagtaa tttgtattga
     2701 tggaattact gaaggcgcag catgtaccgt aatgtgtgct gaggaattaa tcaataacga
     2761 tgaacctctt atgattgcaa actcggatca atggattgat attgatatta atgattatct
     2821 aaattacatg aagcatgagg atttagacgg gcttatcatg acaatgaaag ccgatgaccc
     2881 taaatggtct tatgcggaaa tgaatgaaaa taaccatgtt actcgagttg ttgaaaaaga
     2941 agttgtatcc gatgaagcga ctgtagggat ttataattat aaaaaaggcg ctgatttttg
     3001 tagaagtgtc aaaagaatga ttgctttaga tcaacgctct aatggtgaat tttatgttgc
     3061 acctgcttat acactcatgt atgagaatga aggcgcaaaa attggaattt ataatattgg
     3121 ggaagaagca aacgggatgt atggtttggg tatccctgct gatcttgaat tgttcctttc
     3181 ccttgatgta tcaagaaaag cgttggattt ctaatcatgc acgtagcaaa aaagatctgt
     3241 ttgattgggc atgagtgcgg gtcatataaa gggcaaggag gtatagctac atacattgaa
     3301 ctcaccgcga aaggattcgc aaaacttggt ttagaagttc atgttattta catccatgga
     3361 tccggggtag actgcgatga tataaagtca tggaagataa aaaatgatcc tagcctttat
     3421 aaaatatcac ttgaagtaga caaggtttta gaagaaataa aaccggactt tgtagagtgt
     3481 acagacttta tagggatgat gagttatacg ctttcaaagc gagcgatcag aggtttagat
     3541 tataaatgca tttttataac taatcatcac acggggatta aagaagtctg ggaatgggga
     3601 acacagctta aatttaatga gacagcaaag tcatggatga agtctttata tatagctgaa
     3661 agaacacaat ctcttttatc agatgcaaac ttctcgacat ctaattttct agccagttat
     3721 ttagatgcaa accatattga atcatatcaa gtatcgccgt cttattatga aatgaatgat
     3781 aattttggtg aaacaaataa aaatcactat gattctaatt tattacgtat catatcgctt
     3841 ggcagattcg agttaagaaa aaaacaagag cttcttatta aagctacttg tgaacttctt
     3901 gatgaaggct atgatattga aacaactttg attgggaata gtggcggtga tttatatacg
     3961 catcaggact atatggaata ttgctatagc ctgattccca cagagtataa gcataagttt
     4021 catttttatg acttcatgcc atataagttg cttcagaaga aatataccga atatgatttg
     4081 tttgtaatcc catctccata tgagaatttt ccaaatacag ctcttgaagc tatcaactat
     4141 ggagttacag ttgtcggaag taaatccagt ggcgtagctg atatgatagg ggaggattta
     4201 tccgattact gcttcttgcc tgattcagtt tctgatatta aaagagtgat tttaaaatat
     4261 aaccagttag atgctgaaga aagggcgact ctacgtttga aacaacgtct gtccttaaaa
     4321 aatcttactt gctttgaaaa gagtatacaa gaacgctttg aaaagtatca agctgtagat
     4381 gagctacgta gtgaaaaaaa tattgatgat aaagatatat tgattgtata tcaggacaaa
     4441 aatgggctca tcaataaagt agagtattgc tcagaaattt attactccct tgggagcaaa
     4501 tggaaagact ttatcagaga aattaaatat attgtcttga cctccgagga atatcaattt
     4561 aacaagatag agaactacta tccggcaccc ggaatcatat cctgtttttc atcatatgat
     4621 ccttatggaa cggttagaga aatacaacag gctgaaaaag gatattcatc tttaacatta
     4681 tgtgtagaga caatcccata taatgaaggt gattcaatat ctgaatatat cgctcaagtt
     4741 ttactttcaa caagcgatgt catattcttc attgatgaag aaaagaaagt agaacaaggc
     4801 atcagtatcg atatcaaata cgcgattgat aaaattaagt ttaattattc tggtgaaaaa
     4861 tgaatataat aatccatggg ccgctagaaa aagatttctt atttaaaaac ttaaataaaa
     4921 ttcggcacgc ttcagaagac gatcacataa ttatttctgt ttataacgaa gaacttgaaa
     4981 aaacaaaggt tattatagac agcgtcccag tcagaaataa ggttacatta atcggttctg
     5041 atgatgtttt taatcctggc ttttttaaca ttaacagaca aattaatctg gttagtgccg
     5101 cattaaacgt cattgaagat gaaagtgact ttattctaaa agttcgaatg gatcagacta
     5161 ttaactttcg ttgggttaga aaagttgtaa ctgagaagca tgaagaactg aaggataaat
     5221 tgatcaccac taactgctat accagaaaag atcgtcttta tcatccatct gatatgttcc
     5281 tcgccggcac gaaaaaaaca cttcgtgcat attatccaga agagtttttc caagagactc
     5341 acatggataa tttattgaaa ataaaagaac tagtgctctc cggatataaa ggcggatacc
     5401 ataaatattg gccagaatca cgactgttta tgaattactt atccatatta ggtgaggaat
     5461 tacaggatac agagatctgt agtcagtcac aattgaaaaa gcatgtttat atttttaatt
     5521 catgggatat tggattaaaa tggaaaaaat tccttaaagg caaagttaac gttttaccat
     5581 atttctttgt tttaagacct ttcgaaagag ggcctcttga gaacgcagag aattttttag
     5641 caagtgactt aacaggttat acaaaaaagc ctttgaggga aatgatatat aatagtttag
     5701 caaaattata ttttaaatct ggagcttatt tagcgaaccc aatattcata gataacaaat
     5761 cacttatacc aaaattacta cgaaaaatgt ttgatcattc atataaattc atccctccca
     5821 ttttgcataa tcttatgtat aaagtcgcaa agagagttta ttatgcattc aaataagata
     5881 tatataattg ggtggtcagg ctttattggt cggcaatata ttcattatct tgataaaacg
     5941 ggctttaagg gtgaggtttt tcttaactca cgtgccgacg atgagcaaaa atacacttct
     6001 tctacagata acatcacaat aaaatatgtt aattatgagg agatgattac tgggcttaat
     6061 tgttcaaggt caactgtatt ataccttgca aatatgtact caccgggtga gtcgaatatt
     6121 taccctttga atagtgtcaa agaaaacatt attccattta tcactttttt agaggatatc
     6181 aagaataatg caagcaatat caggttcata tttgcttctt caggagggac tatatatggc
     6241 gatagtcaag aaggggaatg ttgcagcgag aaccacttat tagctccgaa aaccatttat
     6301 gcagctaaca aaatagcgca agagaactat ctcaacgttt atcatgttaa ttatggtctt
     6361 gattatcgga tagccagaat tgcaaaccct tatggcccgg ggcagatatt gaaaggtggt
     6421 caaggattaa taccagcaat acttcgttcg tcattaaata atgaaagcct tcctgtgagt
     6481 ggtgatggaa atggtacaag agattatata tatatagatg atctttgttc actttttttc
     6541 agtctaagta gctatagcgg agcttgtaga acatttaatg cgggctctgg taaagagtat
     6601 agtataatgg aaatcataag ttgctttaac gcagtgcatg acagaccaat aaaataccat
     6661 catgttaaaa ccgaatcttc agttgtaagc cgtattgtcc ttgatatcag tcgtgctaaa
     6721 aatgagctgg gatggagccc taaaacttcc ctatctgatg ggattcgtgc ctttatcaac
     6781 tggtcgattc aagtgatgta gttttccata tttcaataat ctaacctcac atgacataca
     6841 ccactcaaat ctcggctcaa gatgtctcga ttgttatcca aggggcgatt ttcgatacaa
     6901 aaaaacaaaa agttgatgct caatttattg aatacttaga aaaagttatt tcgatattta
     6961 taggttgcga atttattgtt tcgacgtggg aatttcctcg cgaaatatac ttcggtcttt
     7021 gcgaaaaata tccgcaagta aattttgttc tgaatgctga tgttgggcca ataataaaaa
     7081 tagtagatga tgtaccgatt gtcaccaatg ttaataggat gattttttcg acaataagtg
     7141 gactcaggga agttaatcgc aaatatgcta ttaaattgag aacagattct ttcttataca
     7201 atgattcgat actccaatcg ctttattatg ttatgaataa acgcgaattt ttcggattag
     7261 acttggaaaa gagaaatgag cgatatcgga tctttgatca tcatgtaatt aactgtaatc
     7321 tatttgcccg caatccgcgc agtcatttac ctttcttata tcatcctggt gatattttcc
     7381 tggcaggatt aactaaagat ttaattaagc ttttcaatat accattggcg acggaagacc
     7441 taataaaaaa atgcaatagt gtgagaaata cttgctttat gaaacttgta cctgaacaat
     7501 atatttgggt tcagtgtatt aaaatgtcaa gaagtacaga tacgttcgat tattcaaatt
     7561 tcacatatga tgaaaaagaa atcgaaaaat cagagcagta ttatttaaat aattttgtac
     7621 catacactgc agaagaattg ggtttttgtt ggccaaagca tcgagaggtt tattttaaca
     7681 aagggagttc aagtatttat gatcatgaag attggcatca tttgtatcat aaatacattg
     7741 acggttttga acaacctaca agttatgcac ataaaaaaag aagtgtggtt atattcttta
     7801 tgaagatgta ttttttcatt cgtagctctc tgcttaaaat aaaattcatt cgcaaatttg
     7861 cttacaaagt ttttgtaaaa agaggatgac ttatgaaggg atttgtttca acgccctcca
     7921 gtacaacgcg ctttagtgaa gtttttgaat cacgtaattt aattgcagaa cttattcggc
     7981 gagattttaa tgtccgctat aagcaaacag ggcttggagt attatgggca ataattaatc
     8041 caggcgtcaa tattcttctt tatctctttg tatttggttt gttagttagg ttaccaaccc
     8101 cagaatataa tgctccttat gctgcggtgt taatctcagc gattttattt tggaatttat
     8161 tttctactag catgctcaca gcaagtgatg cgctgattaa taacatgcat cttgttacta
     8221 aagtttattt cccaaggtta tctttatgta tagcaagtgt atttgttgca ttgattgatt
     8281 tcttgatagc ttttatcgtg tttgtcccgt tagcattatt gtatcatgtg gacataagtc
     8341 taattagatt gcttttatta atcccatgta taattataac tctgatgctt ggatgtggtt
     8401 ttggctgtgc aatggctata ttgaaattga agtatagaga tattagacat atacttcctc
     8461 taataaccca ggttattttc tttgcgtcac ctattgtata tactctatcc attgttccag
     8521 aacaatataa aatattctat tcttttaacc ccatcactgg gatcgtaatg atgtctcgct
     8581 gggctatctt aggcggagca cccattgact tgacaatgct tacttatagc ctaattagtg
     8641 catttgtcgt tatgtgttta ggtgtcattt attttgttaa aaatgatcga tctgtggcgg
     8701 attacgaatg attgagtttg attcggtaag taagcagtat gaaatcaacc atcgacgggc
     8761 caaaagctta cgcgatgttt tttcatttaa caaaaagcca gatgcaaatg cagaaatatt
     8821 taaagcggtt gatgatgtca gctttagtat tgatagcggt aaatcagtag gtgtcttggg
     8881 acgaaatggt gcgggaaaat caacgctttt aaaattatta acgcgtatca tttccccgac
     8941 atcaggcaaa ataactgtag atggctccat ttcttgtctg ctggaagttg gcgctggttt
     9001 tcaccatgaa ctcagtggct acgaaaatat ttttctatgt ggctcaatct tgggaatgag
     9061 tcgtaaacaa gtcaaatcta aaatagaatc tattattgat ttttctgagg tcgagaaatt
     9121 tattgacgaa cctgttaagt attattcatc aggtatgtat atgcgcttag cttttgcgat
     9181 aggtgctcat cttgactcgg atattctagt gatcgacgaa gctttggctg ttggtgactc
     9241 tcgttttcaa aataaatgtt taaataaaat cgacgagatt agaaaaagtg gaaagactat
     9301 attttttgtt agtcatagca ttgaacaagt aaaaaaagtg tgtgatacca gtattgttat
     9361 ggatcatggg aaattattgt tccagggcaa tactgatgaa gctgaaagaa tttacaatag
     9421 tcttactaat ggtgcgtgag tatgatcatt atttctcata gagggtattg gaagttgagc
     9481 agtgaaaaaa atactgcttt ggctttcaaa cgctcatttt ctttaggtta tggtactgaa
     9541 acagatattc gtgactataa gggtagttta gtcatcagtc atgatatacc agatgagagt
     9601 tcattaagca tagactcttt ttttgaaatt tataatcaat atgatgtcaa aggccctttg
     9661 gcattaaacg taaaatcaga tgggctgcaa aaactaataa aagaaaaatt aaacgagtac
     9721 aaggttagta attatttttt ctttgatatg tctatcccgg atactcttgg gtatttatca
     9781 gaaaaactaa atacgttcgt gcgtgttagt gaatatgaaa cactaaacga tctctatgaa
     9841 gatgctgatg gtgtttggat tgacggtttc aaagaaaata ttataacaga atctttactc
     9901 gaaaaaatta tttctgatgg gaagcgtgct tgtattgtat ccgctgatct gcataaaaac
     9961 gatccagcta cgcaatggaa acttttaaaa agttttaaaa caaatattct tcagtcgtca
    10021 aatattattc tttgtactga tttacctgaa aaagcgtcgg agtttttcta tggataataa
    10081 aattaaagcc gtaatcttcg atatggacgg agttcttatc gaagctaaag actggcatta
    10141 cgaagctcta aatcgcgctt tgggactctt cggtctggag ataaccaagc atgaacatct
    10201 tacaacctat gatggtctac ctactaaaga taagttagtc atgttgtctc gtgataaagg
    10261 tttgtcaaat gcgctgcata cctttattaa tgaaatgaag caacagtata ctatggatat
    10321 ggttcataaa ttttgtaaac ctatgttcca tcatgaattc gccctatcaa tgcttaaaca
    10381 agatgggtac aaattagctg tagcatctaa ttcaatacgg aattccgtag aggtgatgct
    10441 tgataaagct aaactattag cttatttgga gttttttatt tctaatcagg atgtgaaaaa
    10501 aggtaagccg gatcctgaga tgtatactct tgctatttca aagttgggat taagaccttc
    10561 tgaatgctta attgttgaag ataatgagaa tgggataaaa gcagctaaag caagtggtgc
    10621 tcacgttctt gaagttgaaa ctgtatacga tgtaaattat caaaacatca aacaacgtat
    10681 taaaaaaatt gaaaacgatg agggtggaaa atgattaata tcctaattcc aatgagtgga
    10741 gagaatcttt atgaaacctc cagtaatttc atctacccaa aaattttaac tgaagttgca
    10801 aataaaactc tcttggaata ttcgcaatta atatttgata atctgcgaga cgaaaaaaat
    10861 cttatttaca ttgctccaga agataaactt aacactcttg gtttgaaatc aatcattcaa
    10921 actattacag atggtaaagg taaaattgtt gccctccaag ggcaaaccaa gggtgctgtc
    10981 tgtagttgtc ttatggcgat cgatgatctc tcacttgatg acgaattgat tatttcttct
    11041 gcggatcatt atattgacga taatttgcaa aggatcgtta atgattttag ggtcatggac
    11101 gctgacgctg gggttcttac ctttgaatct gtgcatccca aatggtcgtt tgtaacacta
    11161 aatgagcatg gtgcagttgt tcaggccgca gagaaaaaag cgattagcag aacagctgta
    11221 gctgggctgt attattttaa aaaggcgcgt gattttgtcg aggctgcgat gaatctcatt
    11281 agaaaggatg gcgatataaa tggtaatttc tatttgtctt cctgccttaa tgagctcgtt
    11341 ttgttgggta aggttatcaa atgtcgcccg ctacaagatg ctatctatca taacttttat
    11401 gatgcccacg cagttaaatc ttttgagctt gctcagacat ccctttctaa tagcgttaag
    11461 caactcacga attcctacat tgaagcattt aactcgaaag atatagaaga atgtcttttt
    11521 cttttttcag acgatgcagt tcttgttgag cccaacaaaa catttactgg gaaagatgaa
    11581 ataaaggaat tgcttaacga tatttttgtg aacaattcta aactccaatt ccgtgccgat
    11641 aatattattg ctgatgatag taaatctgca atcaagttta cacttgagct tactaacgag
    11701 actgttcatg gcgttgattt cattgaatgg aatagtgcgg gtaaaatcca taaactgact
    11761 gcttttttaa atgattaaaa tagttaccag ttttggaatg tgaactgtga acatttatgg
    11821 atgactttaa ttgatgaaaa atcgtgtgat ttgatatctt tttttttaat taatgattta
    11881 tcgaaatttt ttaaaatcgc acaaattcac gctattcgct gcgaagagct tgagttaaca
    11941 taatgttcac ttctcccagc aggataatgg gcttgctggt aaaccacact agacaggagt
    12001 atgtaatgtc taagcaacaa atcggcgttg tcggtatggc agtg
//

</pre>