LOCUS       AB812040               16295 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: H308a.
ACCESSION   AB812040
VERSION     AB812040.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 16295)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..16295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="H308a"
                     /db_xref="taxon:562"
                     /note="serogroup:O92"
     CDS             751..1518
                     /note="O092_01"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BAQ01368.1"
                     /translation="MMYNCQRHIFLVPLKTVTGQFLDKIKTLSSNADSRFLFLLSIQD
                     YSSYLQAVKNYLWAKNVRIISPRKFYDRTEHWEYITKYAYANIEYDTFSYYFFGDTLR
                     LKSFPQITDESDIYINDYCIDFWVNVKENVSHKEIEKKTANEIICNSLLMGRPLFAPL
                     QKIIFKKRIVPQMIFEHKNSYVCDQLIIYYLIDSGNKVKFIKSPFYRINSESRLFSAT
                     ISFFDVIKQQLYLYAKLRCVIGFPMILLRTIFKFLFN"
     CDS             1534..1908
                     /note="O092_02"
                     /codon_start=1
                     /transl_table=11
                     /product="putative phosphatase"
                     /protein_id="BAQ01369.1"
                     /translation="MKKLIVDIDMTLTKGKGVVGYEDAIVNEEMVEKLREYKNDGFTI
                     VLNTSRNMNSYNNNVGLINKNTLPIIINWLNANNIPYDEIYVGKPWCGHEGFYIDDKA
                     IRPSEFINKSYGEIIELLRGEE"
     CDS             1905..3446
                     /note="O092_03"
                     /codon_start=1
                     /transl_table=11
                     /product="putative phosphotransferase"
                     /protein_id="BAQ01370.1"
                     /translation="MIIILSAQYIEQDLRAEFGEIPPSFLPVGNKRLYSYQINNIRNA
                     NPNEDIFLTLPKSFEVSSWDLRELQERNINIIRIDESFSLGQSIAFTLSSICTTENDN
                     IIIYYGDTLLRDDIKTGNLGRDVIYTSRSDFNYAWMKINNRFEDKDIVFCGILSITNP
                     QLLIRNLISTNFDFEKSLLKYNETNRFEQEHREDWLDFGHLNTFYDSKTIVTTERAFN
                     ELTINKYFVEKRSQKKKKLHAEANWFSNVPETMACYIPMLISHGDEPTGYRYKLEYLY
                     CPTLTELFVFGHHPLDIWEQIISSCFKFITNCYEIKNPQCTVDFYSSLIAKNENRLNE
                     FIKENPRFANLIKDCDGNEYYLKDMLNELHQYINKDSTQSFVHGDFCFSNILYDFKKN
                     DIKVIDPRGIDFDDAINIYGDIRYDLAKILHSAIGKYDYIISDRFFVEDDGVKITLDI
                     AVGGVDLRKIIKKEFKKSIFDYQEILAITITLFLSMLPLHYDRPDRQTAFIATAINLY
                     KELKK"
     CDS             3443..4150
                     /note="O092_04"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ01371.1"
                     /translation="MIIIPMAGLSSRFFNAGFKEPKYKLRAHGKTLFEWSVKTFEKLY
                     QTEKFIFICRDVFDTPKFIVSEMKHVGIKDYEIIVLTAETRGQAETVFLALDKVPDNE
                     PLIIFNIDTYQRHFDYMHESNDAYLEVFKGEGEHWSFAKPVMEGSNLVAQTAEKKRIS
                     ELCSNGVYGFKNKKIFINAYIDLIRSNPGELYIAPMFNFIIKQNMEVKYKEVNIADHI
                     FMGTPNEYNNFLGRADV"
     gene            4143..4943
                     /gene="wzm"
     CDS             4143..4943
                     /gene="wzm"
                     /note="O092_05"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ABC transporter permease"
                     /protein_id="BAQ01372.1"
                     /translation="MFKLAFLDIVGGLKKCHFWSYMAWQEILIRYRRSVLGPFWITVS
                     TAIYVVAISTVFSALFHQDIRHYLLYMALGIMIWNYINQTIVETSDSFISCSGFIKQI
                     PIEKTVFIYQAITRNIYFFLHNVVVLIICLLFLDSTLNLYTVSKAILGFCILTFNLFF
                     VALTLSCLCTRFMDLRQIVLSILQIGMLVTPIMWIPTESMRTKVFILEWNPIYHFVDF
                     IRMSLLPSDFPPAVIHPSLKYIIVFTLINMMVGFIVFAKSRKNISYWV"
     gene            4947..5696
                     /gene="wzt"
     CDS             4947..5696
                     /gene="wzt"
                     /note="O092_06"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen export system, ATP-binding protein"
                     /protein_id="BAQ01373.1"
                     /translation="MKSYVSLENVAVNYPIFDVKSRSLKANFIRLTTGGKVSSNAKSV
                     TVNALKDINIELKHGDRLALIGHNGAGKSTLLKTIAGIYEPSEGKISTTGSIASLLDI
                     NLGMNPELSGRENIYSRGMLLGLSRKEINNISEDIIEFTELADFIDLPVRTYSSGMSV
                     RLAFAISTAVKPEILLLDEIIGAGDANFMERARCRISDLIDSVGILIFSSHSEQDVRD
                     FCNRGIVLNAGSIVFDGSVDQAFEYYKWAKS"
     CDS             5737..6549
                     /note="O092_07"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ01374.1"
                     /translation="MMVAIIMSVYKKDKALPLYWAMQSLLHQTYKNLLIFIRLDGDVE
                     TDVETLLFTLQKNYQNIILSRNSVNLGLGYSLNKLIDTILLDHPDIQFIARMDADDIC
                     HLSRIQRQVECLDKTPSIDVLGTACQEFGLYNRVITKKDTDVLLKKNILKTSPFIHPS
                     VIFRRKIFEDGNRYPLNTFLSEDLALWLDLSLKGYGFGNLNEALLFYRINNVTLKRRS
                     NIKKAISELIIRFRYLKVKKNNIVFNCLWIAGHLILKIMPVCLVRVAYRLFR"
     CDS             complement(6538..7566)
                     /note="O092_08"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BAQ01375.1"
                     /translation="MTPISIVICGPQYKEYSINYTINKINKIFANSEIIISTNDEKLI
                     SDVKENTLVDKIIYCNPTGELPSLKFSDIQNINVNNNINKQIKCAYEGILVAKNELVL
                     RLRTDQIILDSSILELWTKINKIPKAHDKKGRIITSSIFSINPRFSERMAYHISDMLQ
                     LGYKNDLLSYFSAPEYPFEYATWYERNPHHKNSNEFERKFRSRFAVEQWLTLHYIFKE
                     EKNFPISFHNEFNDKIITDFEDIFIEYFIIAHPSDISLRASKFYKAESYYNTQCYSTK
                     ECLFLLARKHPEFTYMIDDYKHKGMNKKYFSILMPFIYAPLSQFLIKRMSNNQKNKIK
                     EILNKLTK"
     CDS             7714..8706
                     /note="O092_09"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BAQ01376.1"
                     /translation="MKKDIKVAVLLYGFLRTADLTVGSLIRNVVDVNNADIFYFGPDS
                     SDSPTQIHKGILDSSGFVKINPKGEAHEIPCGIEDKLVSIYGDKLRNYKLHYKKFDDF
                     NEQTSFVDKNEYLFNLNPARFVSMFYNMQGAYELMNEYEEKQNFRYDVIIITRPDLSF
                     YAPVSAKNIRHGYVYIPAGEGFCPHTGNRNIGLSQVLPYRNRSEGKMVPSGVGFNDQL
                     LVLTGKTASCMENLLDSVLMYMREKVPLTPETLLYYHLVVNNHLKVQYIMEWPYEIVR
                     SDDVETITVTDLMIMDLIDKYHPVVKARISRHPIKYLFKYGRIRWRAFVNRYLR"
     CDS             8763..9971
                     /note="O092_10"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acetyltransferase"
                     /protein_id="BAQ01377.1"
                     /translation="MLIGGYMQSNKYLFAQSNKYLFAHYLRGLAAITVIFAHYGSAYF
                     NANPFLSSIVNVPRLTDTSYPWIIQQFPFDFPGFLATFGVCVFFMISGFVIPISIEKY
                     KCCMFLIKRFFRLFPTYLFVYGVNLLVALMGYLIFKGHGVFYPFDIKDVLSSSLIGIN
                     TFINGVVGLDPVAWTLAIEILFYFTMAVVFNVVFKLKNKREIGLFDILMLSFLLNLCV
                     IKLSKYYDVLFPAGGGLNGASLIKALFFISVMLLGTSFYLHAKGRITARALFFTLVAQ
                     FWGIVYVSMHIHQSAMYIDSSRVFSWIGITILVFSFCYFMDSQIKEHKVFGFFGDISY
                     PLYLCHSYIGYLILGVVSSYLPILPRSFIILLPLPFVITVAWYIHKYVEMPSSRFLPQ
                     KTVINNYFIK"
     gene            10143..11549
                     /gene="gnd"
     CDS             10143..11549
                     /gene="gnd"
                     /note="O092_11"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ01378.1"
                     /translation="MSKQQIGVFGMGVMGRNLALNIESRGYTVSIFNRSREKTEEVVN
                     ENFGKKIVPYYTLKDFVESLETPRRILLMVKAGEGTDVAIDSLKPYLDKGDTIIDGGN
                     TFFKDTIRRNCELSLEGINFIGAGISGGEEGALKGPSIMPGGQREAYELVAPILSKIA
                     AVAEDGVPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAEAYSLLRSGLNLSNEELAQI
                     FTDWNNGELSSYLIDITKDIFIKKDEDGNYLVDIILDEAANKGTGKWTSQSALELGES
                     LSLITESVFARYISSMKDQRVTASKVLSGPQAQPVVDKADFIEKVRRALYLGKILSYA
                     QGFSQLRAASQEYNWDLNYGELAQIFRAGCIIRAQFLQKITDAYAENPRISNLLLAPY
                     FTKIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDFFGA
                     HTYKRTDKDGVFHTEWLK"
     CDS             complement(11741..12244)
                     /note="O092_12"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase of IS1"
                     /protein_id="BAQ01379.1"
                     /translation="MPGNRPHYGRWPQHDFTSLKKLRPQSVTSRIQPGSDVIVCAEMD
                     EQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDG
                     WPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHY
                     LNIKHYQ"
     CDS             12280..12594
                     /note="O092_13;
                     transposase"
                     /pseudogene="unitary"
                     /codon_start=1
                     /transl_table=11
     CDS             12689..13729
                     /note="O092_14"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase of IS481 family"
                     /protein_id="BAQ01380.1"
                     /translation="MLHTTNPVIKHKAGLLNLSEELSNVSKACKIMGVSRDTFYRYCE
                     LVDEGGVDALINRSRRAPNLKNSTYEATEQAVVDYAVAFPAHGQHRTSNELRKQGVFI
                     SGSGVRSVWLRHNLENFKKRLKALEEKVARDGIELTDSQIAALERKASDDEACGEIET
                     AHPGYLCSQDTFYVGNLKGVGRIYQQTFVDTYSKVAHCKLYVTKTPITAADLMNDRVL
                     PFYASQSLPMLRILTDRGTEYCGKVEQHDYQLYLAINDIEHTKTKAMSPQTNGICERF
                     HKTILNEFYQVTFRKKLYGDLDTLQSDLDKWLAHYNNERTHQGKMCNGRTPMETLLDG
                     KRIWAEKNLSQM"
     gene            14570..15736
                     /gene="ugd"
     CDS             14570..15736
                     /gene="ugd"
                     /note="O092_15"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ01381.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVIALDIEPARVDILNKRISP
                     IADSEIQHFLQSGDIRFKATLDKKTAYQNADFVIIATPTDYDPKTNYFNTSSVESVIR
                     DVIEFNPDTVMVIKSTVPIGFTQTMREKFNTQNLIFSPEFLREGTALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKKDIHTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     EALGLNSRQIIGGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYHSVPNNIISAI
                     VDANRTRKDFIADAILARKPRVVGIYRLIMKTGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMTEELFFNSRLERNLAEFKKQADVIISNRMASELLDVEEKVYTRDLFGSD"
ORIGIN      
        1 gtaatcactg agtcgagtga gaagatggcg tccagtacca caatttgtgt caccaccccc
       61 cagaagttcg cacctttgcg ttgtgtggga ttattgctgt ctttcccttc cagccgttcg
      121 ttcagctcca tcgtggcttt gaacaacagg aagaaaccac caaacagcat gattaaatcg
      181 cgggcgctaa aggtaaacga gcggaagctg aacagcggtt gagtcagtgt gaccagccag
      241 gagattgacg tcagcaataa caggcgcatt aacatcgcca gcaggagccc ggtaacccgt
      301 gcgctgtcgc gctgcttagg cggtagtttt tcggcgagga tggcaataaa gaccaggtta
      361 tcaataccga ggaccagttc gatcacaatc aacgtgatta acccagccca gatagacgga
      421 tcggcaatcc attccatagt aagctcaaaa cctatttgtt gtatgataaa tatcacaatc
      481 tgatcatgaa taaatcatgg tagaattgca atatatgatg gaacagtaca cgctcttgca
      541 tctgcgacat ttttaggtga taatacttag aagaatattt atagaacaag taagttgtgc
      601 ttaattatcg acgatagtat actaagcaag ttggtagctt ttaagccaag ggcggtagct
      661 ttcacttttt attatgaaaa agtgccgaaa gcacgcgtgt gaagtgctta aaagatatac
      721 ttaagaagga ttcattgaaa gataggattt ttgatgtata actgccagcg acatattttc
      781 ttggttccgt taaagacagt tactggtcaa tttttagata aaataaaaac actaagcagc
      841 aatgcggata gtcgtttctt atttttgtta agtattcaag attacagtag ttatctacag
      901 gcggtaaaaa actatctttg ggcaaagaac gttaggatta tttcacctag gaaattctat
      961 gatcgtactg agcattggga atatataact aagtatgcgt atgctaatat agaatatgat
     1021 actttttcgt attatttttt tggcgataca ttacgactaa aatcttttcc tcagattact
     1081 gatgagagtg atatttatat caacgactac tgtattgatt tttgggtaaa tgttaaagaa
     1141 aacgtatcgc ataaggaaat tgaaaaaaaa acagctaatg aaataatttg caatagtctt
     1201 ttaatgggaa ggccattatt cgcaccacta cagaaaatta tatttaaaaa aagaatagtt
     1261 ccccaaatga tatttgaaca taagaatagc tatgtttgtg atcaactgat aatttattat
     1321 ctaattgata gcggtaataa agttaaattc attaaatctc cattttatag aataaacagt
     1381 gagagcagat tattttctgc aacaataagc ttttttgatg ttattaaaca acagctttat
     1441 ctttatgcaa aattacgttg tgttattgga ttccctatga ttttgcttcg taccatcttc
     1501 aaatttttat tcaactagat agaaccaaat gttatgaaaa aactaattgt cgatatagat
     1561 atgactctta ctaaaggcaa gggagttgtg gggtatgaag atgctattgt taatgaagaa
     1621 atggtggaaa aattaagaga gtataaaaac gatggtttta ccatagtctt gaatacaagt
     1681 cgaaatatga attcttataa caataatgta ggtcttatta ataaaaatac gttgccgata
     1741 atcattaatt ggcttaatgc gaataacatt ccatatgatg aaatttatgt cgggaaaccg
     1801 tggtgtggac atgagggatt ttacattgat gataaggcta tcaggccaag tgagtttata
     1861 aacaagagtt atggagaaat aattgaatta ttaaggggtg aagagtgatt attatattat
     1921 ctgctcaata tatcgagcag gatttgcgcg ctgaatttgg tgagatccct ccatcatttt
     1981 tacctgtagg aaataaaaga ctttacagct atcagataaa taatatccgt aatgcaaacc
     2041 caaacgaaga catattccta acattaccta aatctttcga agttagttca tgggacttga
     2101 gagagcttca agaacgcaat attaatatta ttagaattga tgagagtttt tctttaggtc
     2161 agtcaattgc ttttactctt tcttcaattt gtacaacaga aaatgataat attattatct
     2221 attatggtga cactttgtta agggatgata taaagactgg caatttaggc agggatgtta
     2281 tctatacttc gcgttcggac tttaactatg cctggatgaa gataaataat aggtttgaag
     2341 ataaagatat tgtcttttgt ggtatattga gcataactaa cccacagttg cttattcgca
     2401 atttaattag tacaaatttt gatttcgaaa aaagtctttt aaaatataat gaaactaata
     2461 gatttgagca ggaacaccgt gaagattggc tggactttgg tcatctaaat actttctatg
     2521 attcaaaaac tattgttact actgaaagag catttaatga gctaacgata aataaatatt
     2581 tcgtcgaaaa gcgttcacag aaaaagaaaa aacttcatgc agaagctaat tggttttcca
     2641 atgttcctga gacaatggca tgctatatac ctatgctaat tagtcatggg gatgaaccaa
     2701 caggatatcg ttataagctt gagtatttat attgtccgac tctaacagaa ttatttgttt
     2761 ttggccatca tccattggat atttgggaac aaatcataag tagttgtttt aaatttatta
     2821 ctaattgtta tgaaataaag aatccacaat gtacagttga tttctactca tcgttgatag
     2881 caaaaaacga gaacaggcta aatgaattta ttaaagaaaa tcctcgcttc gctaatctga
     2941 taaaagactg tgatggcaac gaatactatc ttaaggatat gttaaatgaa ctccatcagt
     3001 atattaataa ggattcaacc caatcatttg ttcacgggga tttttgtttt agcaatatat
     3061 tatatgattt taaaaaaaat gatataaaag ttattgaccc gagaggaatc gattttgatg
     3121 acgcgatcaa catctatggt gacattcgtt atgatttggc caaaatcttg cactcagcaa
     3181 taggtaaata cgattatatt atttcagata gattttttgt cgaagatgat ggtgtcaaga
     3241 ttacgttgga tatcgcagtc ggtggagttg atctaaggaa gatcattaag aaagaattca
     3301 aaaaatcaat atttgattat caagaaatcc ttgcgataac aataacactg ttcttatcaa
     3361 tgttgccatt gcactacgac cgtccagatc gacagaccgc tttcattgct acggctataa
     3421 acctatataa ggagttgaaa aaatgattat tattccgatg gcagggctga gtagtcgctt
     3481 ctttaatgct ggatttaaag agccaaagta taaactcaga gcgcatggta aaacactgtt
     3541 tgagtggtca gtaaaaacat ttgaaaaact ctatcagaca gaaaagttta tttttatatg
     3601 tcgcgatgta tttgataccc ctaagtttat agtatcagag atgaaacatg ttggaataaa
     3661 agattatgaa attatagtcc taactgctga aactcgtggt caggccgaaa ctgtatttct
     3721 tgctttagat aaagttcctg ataatgaacc gttgattatt tttaacattg atacttatca
     3781 acgccacttt gattacatgc atgaatctaa cgatgcgtat ctagaagttt tcaagggaga
     3841 aggagagcat tggtccttcg cgaaaccagt catggaaggc tctaatttag tggctcagac
     3901 ggctgaaaag aaacgcattt cagaattatg ttctaatggc gtctatgggt ttaaaaacaa
     3961 aaaaatcttt attaacgcat atattgattt gattcgtagt aatcccggtg aattgtatat
     4021 agctccaatg tttaatttta ttataaaaca aaacatggaa gtgaaatata aagaagtaaa
     4081 tattgcagac catattttca tgggaacacc aaatgagtat aacaatttct taggtagggc
     4141 tgatgtttaa attagcattt ttggatattg taggcgggtt aaagaaatgt catttctggt
     4201 cttatatggc ttggcaggag attttaatta gatatcgccg tagtgtgctt ggtccttttt
     4261 ggatcacagt aagcacagcg atttatgttg tcgctatttc aaccgttttt tcagcactgt
     4321 ttcatcagga tattcgccat tatcttctct acatggcatt ggggataatg atttggaatt
     4381 atattaacca aacaattgtt gaaacttcgg actcttttat ttcttgctct ggctttatta
     4441 aacagattcc gattgaaaaa actgttttta tataccaagc gatcaccaga aatatatatt
     4501 tttttctgca caacgttgtt gttttaatta tatgtttatt atttttagat agcacgctta
     4561 atttatatac cgttagtaag gcaattctag gtttttgcat tttaacattt aatttattct
     4621 tcgtcgcatt gacattgtca tgcctttgca ctcggtttat ggatttaagg caaattgtgc
     4681 tgtccatctt acagattggc atgctggtta ctcctattat gtggattcca acagagtcaa
     4741 tgcgtacaaa ggtatttata ttagaatgga atcctatata tcatttcgtt gattttattc
     4801 gaatgtcact attaccatct gattttcccc ctgctgtaat acatcctagt ctgaaatata
     4861 ttatcgtgtt tactttaata aatatgatgg ttggttttat cgtatttgca aaatcaagaa
     4921 aaaatatatc ttattgggtt taatttatga agtcttatgt atcattagaa aatgtagctg
     4981 tcaactatcc gatatttgat gttaaatcaa gatcattaaa agctaatttt atccgtttaa
     5041 ctacaggtgg taaggtatca tcaaatgcaa aatccgttac tgtaaatgcg ctgaaagata
     5101 ttaacattga gttaaaacat ggagatcgtc ttgcattgat tgggcacaat ggtgcaggaa
     5161 aatcaacatt attaaagact attgctggaa tttatgaacc ttcagaaggt aaaatatcaa
     5221 caacgggtag tattgcaagt ttacttgata ttaatttagg gatgaatcct gaattatctg
     5281 ggcgggagaa tatctattcg cgtggaatgc tcttaggact gtctaggaaa gaaatcaata
     5341 atattagtga agatattatc gaatttactg aactcgctga ttttatagat ttaccagttc
     5401 gaacttactc ttcaggtatg tcagttaggt tggcgtttgc tatctccaca gctgttaaac
     5461 cggaaatact tttacttgat gagattattg gcgctggtga tgcaaatttt atggaaagag
     5521 cccgttgtcg gatcagtgat ttaattgact ccgttggtat tctcatattt tcttcacatt
     5581 cagaacagga tgttagagat ttttgtaatc gtggtatagt tcttaatgct ggtagtattg
     5641 tgttcgatgg ctctgttgat caggctttcg aatattataa atgggcaaaa tcttgaattc
     5701 atattatgaa aatatcctaa tggaagcatc gataaaatga tggttgcaat aattatgtct
     5761 gtttataaaa aggataaagc actgcccttg tattgggcta tgcaaagtct gttgcaccaa
     5821 acctataaga atttacttat ctttatcaga ttggatggtg atgtagaaac tgatgtggaa
     5881 acgttacttt ttactttaca aaaaaattat cagaacatca ttttatcgag gaatagtgtt
     5941 aatttgggct taggctactc acttaataag ttgattgata ctatcttact tgatcatcct
     6001 gatattcaat ttattgccag aatggatgcc gacgatattt gtcatttgag taggattcag
     6061 cggcaagttg aatgtcttga taagactccc agcattgatg ttttgggcac ggcatgtcaa
     6121 gaatttgggc tttataacag agtgattacc aagaaggata cagatgtact gctaaaaaaa
     6181 aatattttaa aaactagtcc atttatccat ccatcagtta tttttagaag gaaaatattt
     6241 gaagacggca accgatatcc gcttaatacg tttttgtctg aagacttagc tctttggctt
     6301 gatttatcgt taaaaggata tggttttgga aatctcaatg aggctctact tttttatcgt
     6361 ataaataatg taacgctaaa acgtcgatcg aatataaaaa aagctatttc agaattaata
     6421 attaggttta gatatcttaa ggttaaaaaa aataatatag tattcaattg tctttggatt
     6481 gccgggcatc ttatactaaa aataatgccc gtgtgtttag ttagagttgc ataccgatta
     6541 tttcgttagt ttgttcagta tttcttttat tttgtttttc tggttgtttg acattctttt
     6601 tatgagaaac tgtgataacg gagcgtatat aaaaggcatt aatatagaga aatatttttt
     6661 attcattcct ttgtgcttgt aatcatcaat catatacgta aactcagggt gttttcgggc
     6721 taataagaat aggcattctt ttgttgaata gcattgagta ttgtaataac tttcagcttt
     6781 ataaaattta cttgctctta agcttatatc actagggtgg gcaataataa aatattcaat
     6841 aaatatatcc tcgaaatcag ttatgatctt gtcgttgaat tcgttatgaa atgagatcgg
     6901 gaaatttttc tcttctttga agatatagtg tagagttaac cattgctcta cagcaaatct
     6961 tgaacgaaat ttcctttcga attcattact atttttgtga tgaggattcc tttcatacca
     7021 agtggcatac tcaaaagggt attctggagc tgaaaaatag gatagcaaat cattcttgta
     7081 gcccagttgc aacatatcac ttatatgata tgccattctt tcagaaaaac gtgggtttat
     7141 tgaaaaaata ctagatgtaa taattctgcc ttttttatca tgagctttgg gtattttatt
     7201 tatttttgtc caaagttcaa gaatgctact atcaagtata atttgatctg ttcttaagcg
     7261 tagtacgagt tcatttttgg ccaccaatat tccttcgtat gcacatttta tctgtttatt
     7321 aatattattg tttacgttga tgttttgtat atcgctaaac tttaaagaag gtagctcccc
     7381 tgtagggttg caataaatta ttttatctac tagagtgttt tcttttacat cagatataag
     7441 tttttcatcg tttgttgaga ttattatttc actgttagcg aatattttat ttatcttatt
     7501 tattgtgtaa tttatactgt attctttgta ctgtgggcca caaataacaa tgctaatagg
     7561 cgtcatgctt cctccttttt tcttcgtcag atattacatt ttttttatga gagaggcaat
     7621 gtaaatgtag acagttatta ctgataagtg actaaaaaag taatatgtgt tattgatttt
     7681 gcattgagtt gagtaaatat tatacgaggt tttatgaaaa aagatattaa ggtggctgtt
     7741 ttactttatg gatttcttcg gacagcagat cttacagttg gttctctcat tagaaacgtt
     7801 gttgatgtta ataacgctga tatcttctat tttggtcctg attcatcaga tagccccact
     7861 caaattcata aggggatttt agatagctct ggatttgtta aaattaatcc taagggggag
     7921 gctcatgaga taccttgtgg tattgaggat aaattagtta gtatatatgg agataaatta
     7981 agaaattata aattacatta taaaaagttt gatgatttta atgagcaaac atcctttgtt
     8041 gataaaaatg aatatttgtt taatctaaat cctgcacgat ttgttagtat gttttataat
     8101 atgcaggggg cttatgagtt aatgaatgaa tatgaggaaa agcaaaattt tagatatgac
     8161 gtaattatta ttacaagacc tgatctttcg ttctatgcac ctgtttctgc taaaaatata
     8221 cgacatggtt atgtttatat accagcaggt gagggatttt gcccgcatac tggtaataga
     8281 aatattgggt tatcacaagt cttaccatac agaaaccgtt ccgaaggcaa aatggtacca
     8341 tcaggagtag gttttaatga ccaattgttg gtgctcactg gaaaaacagc tagctgtatg
     8401 gagaatcttc ttgattctgt attgatgtat atgagagaaa aagttccttt aactccggag
     8461 acgctcttat actatcatct tgtagtaaat aatcatttaa aagttcaata cataatggag
     8521 tggccatatg aaatagttcg ctcagatgat gttgaaacga ttacagtgac agacttgatg
     8581 attatggatt taattgataa atatcatcct gtcgtgaaag ccagaatcag tcgtcatccc
     8641 ataaaatatt tatttaaata tgggcggatt agatggagag catttgtcaa taggtactta
     8701 agataataat tcttctatat gtgtgttttt ttattagtat cgcgatgcta taaattattt
     8761 gattgttgat aggtggttac atgcagagta ataaatatct atttgcccag agtaataaat
     8821 atctatttgc ccattatctt agggggcttg cagccataac agttatattt gcacattatg
     8881 gttctgcata ttttaatgcg aacccttttt tgtcctcaat tgttaatgtg ccaagactta
     8941 ctgacacatc atatccatgg attatccaac aatttccatt tgattttcct ggctttttag
     9001 caacttttgg tgtttgtgtt ttttttatga ttagtgggtt cgtgattcct atttcgattg
     9061 aaaaatataa atgctgtatg tttttaatta agcgattttt tcggcttttt cctacttatt
     9121 tatttgttta tggagtgaat ctgctggttg cattgatggg gtatctaata tttaaaggac
     9181 atggtgtttt ttatcctttt gatattaaag atgttttgtc atcatcctta ataggaatta
     9241 atacctttat caatggagtg gtggggttgg acccagttgc gtggacgctg gccatcgaga
     9301 ttctgtttta ttttacaatg gcggtagttt ttaacgttgt gtttaaacta aaaaacaagc
     9361 gtgagattgg cttatttgat atcttaatgt tgagttttct tttaaattta tgcgttataa
     9421 aactctctaa atactatgat gtcctcttcc ctgctggggg gggattaaat ggtgcttcac
     9481 tgatcaaagc tctgttcttt atatctgtta tgttgctggg gacgagtttt tacttacatg
     9541 ctaagggccg gattactgct cgagcattat ttttcactct agttgctcaa ttctggggaa
     9601 tagtatatgt aagtatgcat atacatcaat cagccatgta tatagactct tctagagtgt
     9661 ttagttggat agggattaca attcttgttt tttctttttg ttattttatg gattctcaga
     9721 taaaagaaca taaagttttt ggcttttttg gtgatataag ctaccctctt tacctttgcc
     9781 attcctatat tggttattta atcttagggg tggtttctag ttatctacct atactgccgc
     9841 gttcattcat tattttattg ccccttccat tcgttattac tgttgcatgg tatattcaca
     9901 aatatgttga gatgccgagt agtagatttt taccacaaaa aacggttatt aataattact
     9961 ttatcaaata gtcaccgaaa gaaaatacct gttgtttgtt tttatgaatc ccaaagctga
    10021 gttgtttttc taatgcttaa tagcgtatag tggtgtgttt gttaatttag attgaaaatt
    10081 aatatttaca cttaagtttg gtctgaagat agaccttaat ctatgtattg taggagcacg
    10141 taatgtcgaa acaacagatt ggcgttttcg gtatgggggt aatggggcga aatttagcgc
    10201 tcaatatcga aagccgtggt tataccgtat ctattttcaa tcgttcccgt gaaaaaaccg
    10261 aagaggttgt taatgagaat tttggtaaaa aaatagttcc atattatacg ctaaaagact
    10321 ttgttgagtc acttgaaaca cctcgtcgta ttctgctaat ggttaaagca ggtgaaggaa
    10381 ctgatgttgc tatagattct ctcaaaccat accttgataa aggagatacg attattgatg
    10441 gtggtaatac cttttttaaa gataccattc gcaggaattg tgagctttct cttgaaggca
    10501 ttaattttat tggcgcaggt atttcaggtg gagaagaggg agcattgaaa ggaccttcca
    10561 ttatgcctgg cggacagaga gaagcctatg aattggttgc tcctattctg tctaaaattg
    10621 ccgcagtagc cgaagatggt gtcccatgtg ttacctacat cggtcctgac ggagcgggtc
    10681 attatgtgaa gatggttcac aatggaattg aatatggcga tatgcagtta atcgcagaag
    10741 cttattcttt gctgcgaagt ggtctgaatc tgagcaatga agaacttgca cagatattta
    10801 ctgattggaa taacggtgaa ctgagcagtt atctgatcga tattactaag gatatcttca
    10861 tcaaaaaaga tgaagatggt aattacctcg ttgacattat tcttgatgaa gctgctaaca
    10921 aagggactgg caaatggacc agccagagcg ctctggagct cggcgagtcg ctgtcgttga
    10981 taactgagtc tgtgtttgca cgttacattt cttctatgaa agatcagcgt gttaccgcgt
    11041 ctaaagtcct ttccggaccg caagcgcaac cggtcgttga taaggcagat ttcattgaaa
    11101 aggtccgtcg tgcattgtac ttgggaaaaa tcctctccta tgctcagggc ttctcacaat
    11161 tacgtgctgc ttcgcaagag tacaactggg atttgaatta tggcgagcta gcccaaattt
    11221 tccgcgcagg ctgcattatc cgtgctcagt tcctgcagaa aatcactgat gcttatgctg
    11281 aaaatcctcg gatttctaac ttgcttttgg caccgtactt cacgaaaatt gcggatgatt
    11341 atcaacaggc tctgcgtgac gttgttgctt atgcggtcca gaatggtatt ccagttccaa
    11401 ctttcgctgc agcagtggca tattatgaca gctatcgtgc agctgtcctc ccagccaatt
    11461 tgatacaggc gcagcgcgat ttttttggtg cgcatactta taagcgtact gacaaagatg
    11521 gtgtattcca tacagaatgg cttaaatagt cacgaataac taatctgaaa aaactccaag
    11581 aagcccggcg cttgcactcg ggctttttga cctttcctga atagttttga tttgtgtctt
    11641 atttaaatcc ggagaacgtg atgatgactc aattcaacct tgccttacgt cattattctc
    11701 tatcctttaa atagttcgaa aaaagggtaa tgactccaac ttactgatag tgttttatgt
    11761 tcagataatg cccgatgact ttgtcatgca gctccaccga ttttgagaac gacagtgact
    11821 tccgtcccag ccttgccaga tgttgtctca gattcaggtt atgtcgctca atgcgctgag
    11881 tgtaacgctt gctgattacg tgcagctttc ccttcaggcg ggattcatac agcggccagc
    11941 catccgtcat ccataccacg acctcaaagg ccgacagcag gctcagaaga cgctccagtg
    12001 tggccagagt gcgttcaccg aagacgtgcg ccacaaccgt cctccgtatc ctgtcatacg
    12061 cgtaaaacag ccagcgctga cgtgatttag caccgacgta gccccactgt tcgtccattt
    12121 cagcgcagac aatcacatca ctgcccggct gtatgcgcga ggttaccgac tgcggcctga
    12181 gttttttaag tgacgtaaaa tcgtgttgag gccaacgccc ataatgcggg cggttgcccg
    12241 gcatccaaca ccattcatgg ccatatcaat gattttctgg tgtgtaccgg gttgagaagc
    12301 ggtgtaagtg aactgcagtt gccatgtttt acggcagtga gagcagagat agcgctgatg
    12361 tccggcagta cttttaccgt tacgcaccac gccttcagta gcggagcagg agggacaact
    12421 gatggagatg gaagccacgg gagcacctca aaaacaccat catacactaa atcagtaagt
    12481 tggcaccatt accggaaagg actgaagaaa ttatgcgtgg cagtctcctt tcggtccata
    12541 atagctgaac aaaagaaaga acccgaaatg acggtcagat attatatgta ttagtcgcga
    12601 cttgatctga cactgagcct tgaaaggttg agagttaccg gttttgatat gggtgtcgaa
    12661 tccttataca aaacacgagg taactctcat gcttcatact accaatcccg ttatcaaaca
    12721 caaagccggt ttgctcaatc tgtctgaaga actcagcaac gtatcaaaag cctgtaaaat
    12781 catgggcgtt tcgcgtgata cgttttatcg ttactgcgaa ctggtcgatg aaggtggtgt
    12841 ggatgcgctg attaatcgta gtcgccgtgc tcctaacctt aagaacagta cctatgaggc
    12901 aactgaacag gctgttgttg attacgccgt cgctttcccg gcacacggtc agcaccggac
    12961 cagcaacgag ctgcgtaaac agggcgtttt tatctccggc agtggtgtcc gttctgtctg
    13021 gttacgccat aaccttgaga atttcaaaaa acgcctgaaa gcactggaag aaaaagtagc
    13081 ccgtgatggc attgagctca ctgacagcca gatcgcagca ctggagcgta aagccagtga
    13141 tgacgaggcc tgtggcgaaa tcgaaacggc tcatccggga tatctgtgtt cgcaggacac
    13201 gttctatgta ggcaacctga aaggtgtcgg gcgtatttat cagcagacgt tcgttgatac
    13261 gtactcgaaa gtggctcact gtaagcttta cgtcaccaaa acaccgatta ccgcggctga
    13321 cttaatgaat gatcgtgtac tgccgtttta tgcgtctcag agcctgccga tgctgagaat
    13381 actgaccgac agaggcacgg agtactgtgg caaagtagag cagcatgatt atcagcttta
    13441 tctggcaata aacgatatag aacacacgaa aacaaaggcg atgtccccgc agaccaacgg
    13501 catctgcgag cggttccata aaacaatcct gaacgaattt tatcaggtaa cgttccgtaa
    13561 aaaactgtac ggcgatcttg atacattaca gtcggatctt gataaatggc tggctcacta
    13621 taataatgag cgaacccatc agggaaaaat gtgcaatggg cggacgccaa tggaaacgtt
    13681 acttgatgga aagcgcatct gggccgagaa aaatttaagc cagatgtaat ctgacagata
    13741 cctgtataaa taaccggtaa ctgtcagatc aggtctgagc taatacatat tatatctgtt
    13801 ctgccgattt aaccgctgag aagttcgcta tagcgatccg aaatcacggg taggtggagg
    13861 ataagccaaa ctgatgtctg gatgtggtaa tgaatgaaga cgactgcaaa ataagaagag
    13921 gaaaagcagc catggacaga aactatctgg cgtcagtcct tgctgggacc gggttttcgt
    13981 aatcttgccc tgctcagtgc acacttctcc tgactaaggt cggcaaacat atgttttaaa
    14041 cgtcgattct cgccctaaaa atccttgatc tttttaacat tagaagcctc catgccactg
    14101 tatctggatt tcccgttgta gtaagaggct tcagatatac cggcttaccg gcggttatat
    14161 ttaacagttc gtccagcttc aatcgactta accacagaaa tgagcttatg ctcagtaaaa
    14221 tgggttttac acatagcaat ctccttcgtg ggctgattga ttatgtcgga tgatctccca
    14281 atataaatgg cacggttata ggggaggctt acaattgatc tgaccgcata accgggctga
    14341 gtttcaccaa agctatctac tattctacga aactatttag acaattaatc cgattggttt
    14401 tatccagtct cttaatatta tcttaatcac tgactgttaa cccgataaag aaaacatgca
    14461 ttgttttctg cttagttgaa taccactctt cttaccgcta aaactgttaa cttctcgctg
    14521 gaatattaaa atatcatgat taattagtaa gataatttgt ggacataaaa tgaaaatcac
    14581 catttctggt accggatatg ttggcctgtc taacggcttg cttatcgcgc aaaaccatga
    14641 agttatagca ttagatattg agcctgcgcg cgttgatata ttaaataagc gaatctctcc
    14701 tattgcagat agcgaaattc agcatttttt acaatcagga gatatacggt ttaaagcgac
    14761 attagataaa aaaactgcct accagaatgc cgattttgtg atcattgcga cacctacgga
    14821 ttatgatcct aaaacgaatt atttcaacac ctcaagcgta gagtcggtta tccgggatgt
    14881 catcgagttt aaccctgata cagtcatggt catcaagtcc acggtgccga ttgggttcac
    14941 gcaaaccatg cgtgagaagt ttaatactca gaatctcatt ttctccccgg agttcctgcg
    15001 tgaaggtaca gcactttacg acaatcttca tccttccagg atagttatcg gtgagcgttc
    15061 tgaacgcgca gagcgcttcg ccgcgctatt acaggaaggc gcaattaaga aagatatcca
    15121 tactttattt acggactcca ctgaggcaga ggccattaag ctttttgcta atacttatct
    15181 ggcgatgaga gtagcttatt ttaatgaact ggatagctac gctgaagccc taggattaaa
    15241 ttcccgtcag ataattggag gtgtctgtct tgacccgcgt atcggtaatc actacaataa
    15301 tccctccttt ggctatggcg gatattgttt accaaaagat acgaaacaac tgctggccaa
    15361 ttaccattca gtgccgaaca atattatttc tgccatcgtc gacgctaacc gtacacgtaa
    15421 agattttatc gcggatgcca ttttggcgag aaaaccgcgt gtggtgggta tttatcgact
    15481 gataatgaaa acgggttcag ataacttccg tgcctcctca attcagggaa ttatgaagcg
    15541 tatcaaggcg aaaggcgtgg aagttatcat ttatgaacct gttatgacgg aagagctgtt
    15601 ttttaactcc cgtctggagc gtaatctcgc ggaatttaaa aaacaggctg atgtcattat
    15661 ctcaaatcgc atggcgagcg agctgctcga tgttgaagag aaggtctata cgcgcgatct
    15721 gtttggcagt gactaagagc ggatgaagag tacaaattgg gctaaataat agcccgattt
    15781 actgtaatta ttgatgcctt ttacgcaggt tctcaatcac tgtcgttaac ttcagaccct
    15841 gatcatgtaa caataccaac agatgataca tcaagtcaga tgcctcgttg gtcaactcaa
    15901 agcggtcatg taccgttgct gctagtgcgg tttccacgcc ttcttcgccc accttctgcg
    15961 caatgcgctt agtgccgctg gcatacagtt tggcagtgta agaggtttcg ggatcggcag
    16021 atttgcgctc ggcgagcagt tgttccagtt gatacaggaa cagccactgg tgagcagtgt
    16081 cgccgaagca gctgctggtg cctttgtggc aggttggccc gatgggattc gccagcacca
    16141 gtaatgtgtc gttgtcgcag tccggcgcaa tatttactac gttgaggaaa ttgcccgacg
    16201 tttcgccttt ggtccacagt cgctgtttag tgcgcgagaa gaagatgact ttgccgcttt
    16261 cgatggtttt gtctaaggct tccgggttca tatag
//