<pre>LOCUS       ECORFBM                13316 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  Escherichia coli rfb gene cluster encoding phosphomannomutase,
            GDP-mannose pyrophosphorylase, mannosyltransferase, etc.
ACCESSION   D43637 D13231
VERSION     D43637.1
KEYWORDS    mannosyltransferase A; mannosyltransferase B; mannosyltransferase
            C; GDP-mannose pyrophosphorylase; phosphomannomutase; integral
            membrane component of ABC-transporter; rfb gene cluster; rfbK;
            rfbM; mtfA; mtfB; mtfC; hisIE; hisF; ATP-binding component of
            ABC-transporter.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Sugiyama,T., Kido,N., Komatsu,T., Ohta,M., Jann,K., Jann,B.,
            Saeki,A. and Kato,N.
  TITLE     Genetic analysis of Escherichia coli O9 rfb: identification and DNA
            sequence of phosphomannomutase and GDP-mannose pyrophosphorylase
            genes
  JOURNAL   Microbiology (Reading, Engl.) 140 (PT 1), 59-71 (1994)
   PUBMED   8162191
REFERENCE   2
  AUTHORS   Kido,N., Torgov,V.I., Sugiyama,T., Uchiya,K., Sugihara,H.,
            Komatsu,T., Kato,N. and Jann,K.
  TITLE     Expression of the O9 polysaccharide of Escherichia coli: sequencing
            of the E. coli O9 rfb gene cluster, characterization of mannosyl
            transferases, and evidence for an ATP-binding cassette transport
            system
  JOURNAL   J. Bacteriol. 177 (8), 2178-2187 (1995)
   PUBMED   7536735
REFERENCE   3  (bases 1 to 13316)
  AUTHORS   Kido,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-1994) Nobuo Kido, School of Informatics and
            Sciences, Nagoya University, Dept. of Natural Science Informatics;
            Chikusa-ku, Nagoya, Aichi 464-01, Japan
            (E-mail:j45811a@nucc.cc.nagoya-u.ac.jp, Tel:052-789-4816,
            Fax:052-789-4818)
COMMENT     On Dec 7, 1994 this sequence version replaced gi:474302.
            D13231:Submitted (16-SEP-1992) to DDBJ by:
            Tsuyoshi Sugiyama.
FEATURES             Location/Qualifiers
     source          1..13316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="F719"
                     /db_xref="taxon:562"
                     /clone="pNKB26"
     regulatory      209..214
                     /regulatory_class="minus_35_signal"
     regulatory      232..237
                     /regulatory_class="minus_10_signal"
     regulatory      306..309
                     /regulatory_class="ribosome_binding_site"
     gene            317..1732
                     /gene="rfbM"
     CDS             317..1732
                     /gene="rfbM"
                     /EC_number="2.7.7.22"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /citation=[1]
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphorylase"
                     /protein_id="BAA07745.1"
                     /translation="MLLPVIMAGGTGSRLWPMSRELYPKQFLRLFGQNSMLQETITRL
                     SGLEIHEPMVICNEEHRFLVAEQLRQLNKLSNNIILEPVGRNTAPAIALAALQATRHG
                     DDPLMLVLAADHIINNQPVFHDAIRVAEQYADEGHLVTFGIVPNAPETGYGYIQRGVA
                     LTDSAHTPYQVARFVEKPDRERAEAYLASGEYYWNSGMFMFRAKKYLSELAKFRPDIL
                     EACQAAVNAADNGSDFISIPHDIFCECPDESVDYAVMEKTADAVVVGLDADWSDVGSW
                     SALWEVSPKDGQGNVLSGDAWVHNSENCYINSDEKLVAAIGVENLVIVSTKDAVLVMN
                     RERSQDVKKAVEFLKQNQRSEYKRHREIYRPWGRCDVVVQTPRFNVNRITVKPGGAFS
                     MQMHHHRAEHWVILAGTGQVTVNGKQFLLTENQSTFIPIGAEHSLENPGRIPLEVLEI
                     QSGSYLGEDDIIRIKDQYGRC"
     regulatory      1698..1703
                     /regulatory_class="minus_35_signal"
                     /gene="rfbM"
     regulatory      1718..1723
                     /regulatory_class="minus_10_signal"
                     /gene="rfbM"
     regulatory      1741..1743
                     /regulatory_class="ribosome_binding_site"
     gene            1755..3137
                     /gene="rfbK"
     CDS             1755..3137
                     /gene="rfbK"
                     /EC_number="5.4.2.8"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /citation=[1]
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="BAA07746.1"
                     /translation="MTQLTCFKAYDIRGELGEELNEDIAYRIGRAYGEFLKPGKIVVG
                     GDVRLTSESLKLALARGLMDAGTDVLDIGLSGTEEIYFATFHLGVDGGIEVTASHNPM
                     NYNGMKLVRENAKPISGDTGLRDIQRLAEENQFPPVDPARRGTLRQISVLKEYVDHLM
                     GYVDLANFTRPLKLVVNSGNGAAGHVIDEVEKRFAAAGAPVTFIKVHHQPDGHFPNGI
                     PNPLLPECRQDTADAVRAHQADMGIAFDGDFDRCFLFDDEASFIEGYYIVGLLAEAFL
                     QKQPGAKIIHDPRLTWNTVDIVTRSGGQPVMSKTGHAFIKERMRQEDAIYGGEMSAHH
                     YFRDFAYCDSGMIPWLLVAELLCLKNSSLKSLVADRQAAFPASGEINRKLGNAAEAIA
                     RIRAQYEPAAAHIDTTDGISIEYPEWRFNLRTSNTEPVVRLNVESRADTALMNEKTAE
                     LLNLLKEESL"
     regulatory      3123..3126
                     /regulatory_class="ribosome_binding_site"
                     /gene="rfbK"
     gene            3143..3928
                     /gene="ORF261"
     CDS             3143..3928
                     /gene="ORF261"
                     /citation=[2]
                     /codon_start=1
                     /transl_table=11
                     /product="putative integral membrane component of
                     ABC-transporter"
                     /protein_id="BAA07747.1"
                     /translation="MFSAIYRYRGFIIDSVKRDFQSRYQTSFLGAAWLILQPIAMISV
                     YTLIFSELMRARLAGMDGPFAYSIYLCSGVLTWGLFTETLGNLVNVFLTNANILKKLS
                     FPRICLPIIVTASAFINFLIIFGLFVLFLIVTGNFPGMIFFEIIPVLIVQMLFTLGLG
                     IILGVLNVFVRDVGQFVNILLQFWFWFTPIVYVSKTLPEWVSGLLAYNPMATIIGSYQ
                     NVMLYHQSPNWLALLPVAVLSVILFLFAWRLFKKHAADIVDEI"
     regulatory      3916..3918
                     /regulatory_class="ribosome_binding_site"
                     /gene="ORF261"
     gene            3931..5226
                     /gene="ORF431"
     CDS             3931..5226
                     /gene="ORF431"
                     /citation=[2]
                     /codon_start=1
                     /transl_table=11
                     /product="putative ATP binding component of
                     ABC-transporter"
                     /protein_id="BAA07748.1"
                     /translation="MSIKVQHVGKAYKYYPSKWSRVIEKLLPGDKPRHSKKWVLKDIN
                     FSIEPGEAVGIVGVNGAGKSTLLKLLTGTTQPTKGSIEIQGRVAALLELGMGFHPDFT
                     GRQNVYMSGLMMGLGREEIERLMPEIEAFADIGDYIEEPVRIYSSGMQMRLAFAVATA
                     SRPDILIVDEALSVGDSRFQAKCYARIADFKEQGTTLLLVSHSAGDIVKHCDRAIFLK
                     NGDICMDGTARDVTNRYLDELFGKPDKDSATKSATAISSASGESQMSLDEIEDVYHTR
                     PGYRPEEYRWGQGGAKIIDYHIQSAGVDFPPSLTGNQQTDFLMKVVFEYDFDCVVPGI
                     LIKTLDGLFLYGTNSFLASEGRENISVSRGDVRVFKFSLPVDLNSGDYLLSFGISAGN
                     PQTDMTPLDRRYDSIILHVTKSMDFWGVIDLKSSFTSYQ"
     regulatory      5237..5241
                     /regulatory_class="ribosome_binding_site"
     gene            5246..7372
                     /gene="ORF708"
     CDS             5246..7372
                     /gene="ORF708"
                     /codon_start=1
                     /transl_table=11
                     /product="unknown"
                     /protein_id="BAA07749.1"
                     /translation="MTKDLNTLVSELPEIYQTIFGHPEWDGDAARDCNQRLDLITEQY
                     DNLSRALGRPLNVLDLGCAQGFFSLSLASKGATIVGIDFQQENINVCRALAEENPDFA
                     AEFRVGRIEEVIAALEEGEFDLAIGLSVFHHIVHLHGIDEVKRLLSRLADVTQAVILE
                     LAVKEEPLYWGVSQPDDPRELIEQCAFYRLIGEFDTHLSPVPRPMYLVSNHRVLINDF
                     NQPFQHWQNQPYAGAGLAHKRSRRYFFGEDYVCKFFYYDMPHGILTAEESQRNKHELH
                     NEIKFLTQPPAGFDAPAVLAHGENAQSGWLVMEKLPGRLLSDMLAAGEEIDREKILGS
                     LLRSLAALEKQGFWHDDVRPWNVMVDARQHARLIDFGSIVTTPQDCSWPTNLVQSFFV
                     FVNELFAENKSWNGFWRSAPVHPFNLPQPWSNWLYAVWQEPVERWNFALLLALFEKKA
                     KLPSAEQQRGATEQWIIAQETVLLELQSRGRNESAGSEALRGQIHTLEQQMAQLQSAQ
                     DAFVEKAQQPVEVSHELTWLGENMEQLAALLQTAQAHAQADVQPELPPETAELLQRLE
                     AANREIHHLSNENQQLRQEIEKIHRSRSWRMTKGYRYLGLQIHLLRQYGFVQRCKHFI
                     KRVLRFVFSFMRKHPQVKHTAVNGLHKLGLYQPAYRLYRRMNPLPHSQYQADAQILSQ
                     TELQVMHPELLPPEVYEIYLKLTKNK"
     regulatory      7439..7443
                     /regulatory_class="ribosome_binding_site"
     gene            7458..9905
                     /gene="mtfA"
     CDS             7458..9905
                     /gene="mtfA"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /citation=[2]
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase A"
                     /protein_id="BAA07750.1"
                     /translation="MSRAIIENAGEHRVSILINGMYPIDNINDVKMAYRDLLTDEDMF
                     IFSAVAPTAYRHIENHGRSKAAQAARDIAIANIAPDIVYVISFFEGHSDSYTVSIPAD
                     NVPWKTVCVCHDLIPLLNKERYLGDPNFREFYMNKLAEFERADAIFAISQSAAQEVIE
                     YTDIASDRVLNISSAVGEEFAVIDYSAERIQSLKDKYSLPDEFILSLAMIEPRKNIEA
                     LIHAYSLLPAELQQRYPMVLAYKVQPEQLERILRLAESYGLSRSQLIFTGFLTDDDLI
                     ALYNLCKLFVFPSLHEGFGLPPLEAMRCGAATLGSNITSLPEVIGWEDAMFNPHDVQD
                     IRRVMEKALTDEAFYRELKAHALAQSAKFSWANTAHLAIEGFTRLLQSSQETDAGQAE
                     SVTASRLQMMQKIDALSEVDRLGLAWAVARNSFKRHTRKLLVDISVLAHDDAKTGIQR
                     VSRSILSELLKSGVPGYEVSAVYYTPGECYRYANQYLSSHFPGEFGADEPVLFSKDDV
                     LIATDLTAHLFPELVTQIDSMRAAGAFACFVVHDILPLRRPEWSIEGIQRDFPIWLSC
                     LAEHADRLICVSASVAEDVKAWIAENRHWVKPNPLQTVSNFHLGADLDASVPSTGMPD
                     NAQALLAAMAAAPSFIMVGTMEPRKGHAQTLAAFEELWREGKDYNLFIVGKQGWNVDS
                     LCEKLRHHPQLNKKLFWLQNISDEFLAELYARSRALIFASQGEGFGLPLIEAAQKKLP
                     VIIRDIPVFKEIAQEHAWYFSGEAPSDIAKAVEEWLALYEQNAHPRSENINWLTWKQS
                     AEFLLKNLPIIAPAAKQ"
     regulatory      9938..9942
                     /regulatory_class="ribosome_binding_site"
     gene            9952..11097
                     /gene="mtfB"
     CDS             9952..11097
                     /gene="mtfB"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /citation=[2]
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase B"
                     /protein_id="BAA07751.1"
                     /translation="MKIIFATEPIKYPLTGIGRYSLELVKRLAVAREIEELKLFHGAS
                     FIDQIPQVENKSDSKASNHGRLLAFLRRQPLLIEAYRLLHPRRQAWALRDYKDYIYHG
                     PNFYLPHRLERAVTTFHDISIFTCPEYHPKDRVRYMEKSLHESLDSAKLILTVSDFSR
                     SEIIRLFNYPADRIVTTKLACSSDYIPRSPAECLPVLQKYQLAWQGYALYIGTMEPRK
                     NIRGLLQAYQLLPMETRMRYPLILSGYRGWEDDVLWQLVERGTREGWIRYLGYVPDED
                     LPYLYAAARTFVYPSFYEGFGLPILEAMSCGVPVVCSNVTSLPEVVGDAGLVADPNDV
                     DAISAHILQSLQDDSWREIATARGLAQAKQFSWENCTTQTINAYKLL"
     gene            11398..12222
                     /gene="mtfC"
     CDS             11398..12222
                     /gene="mtfC"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /citation=[2]
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase C"
                     /protein_id="BAA07752.1"
                     /translation="MDMLHLSARPDARTVVTYHSDIVKQKRLMKLYQPLQERFLASVD
                     CIVASSPNYVASSQTLKKYQDKTVVIPFGLEQHDVQHDSQRVAHWRETVGDNFFLFVG
                     AFRYYKGLHILLDAAERSRLPVVIVGGGPLEAEVRREAQQRGLSNVVFTGMLNDEDKY
                     ILFQLCRGVVFPSHLRSEAFGITLLEGARFARPLISCEIGTGTSFINQDKVSGCVIPP
                     NDSQALVEAMNELWNNEETSNRYGENSRRRFEEMFTADHMIDAYVNLYTTLLESKS"
     regulatory      12232..12252
                     /regulatory_class="terminator"
     gene            complement(12261..12869)
                     /gene="hisIE"
     CDS             complement(12261..12869)
                     /gene="hisIE"
                     /codon_start=1
                     /transl_table=11
                     /product="hisIE ORF"
                     /protein_id="BAA07753.1"
                     /translation="MLTELQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVNDRFELTNEASDLMYHLLVLLQDQGLDLGEVIDNLKNRH"
     gene            complement(12863..>13315)
                     /gene="hisF"
     CDS             complement(12863..>13315)
                     /gene="hisF"
                     /codon_start=1
                     /transl_table=11
                     /product="hisF ORF"
                     /protein_id="BAA07754.1"
                     /translation="DPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRT
                     RVTQWETLDWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGA
                     GTMEHFLEAFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
ORIGIN      
        1 aaatgcggaa agtgtggtgc gtttggcggc caggtgaggg aagagcatgt taaaagcaga
       61 gatatgcctg aaggtatatc tctgctttta ggttttcagg acacgcgttc cgcccggctg
      121 tcattgtagc cgctgactga acgatctgcc gacagcctga agcgaaagag ggtggcgttt
      181 agcctggagc acgggagcag tacgcatttt ggttgcgaca gccaatggcg gtagcgtgcc
      241 tgaaaaaggc cgttccaccc agacggtcga ttgattgata gtaaaataaa gcaattattt
      301 atttaaggat ttcattatgt tactccctgt aattatggct ggtggtaccg gcagtcgtct
      361 ctggccgatg tctcgcgagc tttacccgaa acagttcctc cgcctgttcg ggcagaactc
      421 catgctgcag gaaaccatca cccgactctc gggccttgaa atccatgaac cgatggtcat
      481 ctgtaacgaa gagcaccgct tcctggtggc cgaacagctg cgccagctca acaagctgtc
      541 gaacaacatt attcttgagc cggtcgggcg caacaccgcc ccggccatcg ccctggcggc
      601 ccttcaggcc acccgtcacg gcgatgaccc gctgatgctg gtcctcgccg ccgaccatat
      661 catcaataac cagccggtct tccacgacgc catccgcgtc gccgagcagt atgccgatga
      721 aggccatctg gtcaccttcg gtatcgtgcc gaacgccccg gaaaccggct acggctacat
      781 ccagcgcggc gtggccctca ccgacagcgc ccacaccccg taccaggtgg cccgcttcgt
      841 ggagaagccg gaccgcgagc gcgccgaggc ctacctcgcc tccggggagt actactggaa
      901 cagcggcatg tttatgttcc gcgccaaaaa atacctctcc gagctggcca aattccgccc
      961 ggatatcctc gaagcctgcc aggccgcggt caatgccgcc gataacggca gcgacttcat
     1021 cagcatcccg catgacattt tctgcgagtg cccggacgag tccgtggact acgcggtgat
     1081 ggagaaaacc gccgacgcgg tggtggtcgg tctcgatgcc gactggagcg acgtcggctc
     1141 ctggtccgcc ctgtgggagg tcagcccgaa agacgggcag ggcaacgtcc tcagcggcga
     1201 cgcgtgggtg cacaacagcg aaaactgcta catcaacagc gacgagaagc tggtggcggc
     1261 cattggcgtg gagaacctgg tgattgtcag caccaaggat gccgtgctgg tgatgaaccg
     1321 tgagcgttcc caggacgtga agaaggcggt cgagttcctc aagcagaacc agcgcagcga
     1381 gtacaagcgc caccgcgaga tttaccgtcc ctggggccgc tgcgacgtgg tggtccagac
     1441 cccgcgcttc aacgtcaacc gcatcaccgt gaaaccgggc ggcgccttct cgatgcagat
     1501 gcaccaccac cgcgccgagc actgggtcat tctcgccggc accggccagg tgacggtcaa
     1561 cggcaagcag ttcctgctga ccgagaacca gtccaccttt attccgattg gcgccgagca
     1621 cagcctggaa aacccgggcc gcattccgct ggaagtgctg gagatccagt cggggtcgta
     1681 cctcggcgag gacgacatta ttcgtattaa agaccagtat ggtcgttgct aattttttcg
     1741 ggacaaaacg cagaatgaca cagttaacat gctttaaggc ttatgacatc cgtggtgaac
     1801 tgggcgagga gctgaacgag gacatcgcct accgtatcgg ccgcgcctat ggcgaatttc
     1861 tgaaacccgg gaagatagtg gtggggggcg atgtgcgcct caccagcgag tcgctgaagc
     1921 tggcgctggc ccgcgggctg atggacgccg gcaccgacgt gctggatatt ggcctgagcg
     1981 gcacggaaga gatttacttc gccactttcc acctcggggt ggacggcggc atcgaggtga
     2041 cggccagcca caacccgatg aactacaacg gcatgaagct ggtgcgcgag aacgcgaagc
     2101 ccatcagcgg cgacaccggc ctgcgggata tccagcgcct ggcggaggag aatcagttcc
     2161 cgccggtgga cccggcgcgt cgcgggaccc tgcgccagat ttcggtgctg aaggagtacg
     2221 ttgaccacct gatgggctat gtggacctgg cgaacttcac ccgtccgctg aagctggtgg
     2281 tgaactccgg caacggggcg gcggggcacg tgattgatga ggtggagaaa cgcttcgcgg
     2341 cggccggggc gccggtgacc tttatcaagg tgcatcacca gccggacggc cattttccga
     2401 acggtatccc gaacccgctg ctgccggagt gccgccagga caccgccgac gcggtgcgtg
     2461 cacatcaggc ggacatgggt atcgcctttg acggcgactt cgaccgctgc ttcctgttcg
     2521 atgacgaggc gtcgtttatc gaggggtact acattgtcgg cctgctggcg gaggcgttcc
     2581 tgcagaagca gccgggggcg aaaatcattc acgacccgcg tctgacgtgg aacacggtgg
     2641 acatcgtgac ccgcagcggc ggccagccgg tgatgtcgaa gaccgggcat gcgttcatca
     2701 aggagcggat gcgccaggaa gacgctatct acggcgggga gatgagtgcg caccattact
     2761 tccgcgactt cgcctactgc gacagcggga tgatcccgtg gctgctggtg gcggagctgc
     2821 tgtgcctgaa gaacagttcg ctgaaatcgc tggtggcgga ccgccaggcg gcgttcccgg
     2881 cgtcggggga gatcaaccgc aagctgggaa atgcggcgga ggcgatagcg cgcatccggg
     2941 cgcagtatga gccggcggcc gcacacatcg acacaacgga cggtatcagt attgaatacc
     3001 ctgagtggcg ctttaacctg cgcacgtcca acacggagcc ggtggtgcgt ctgaacgttg
     3061 agtccagagc ggatactgcg ttaatgaatg agaaaaccgc cgagctgctc aacctgttaa
     3121 aagaggaatc gctttgagtc ctatgttttc agcgatctat cgctaccgtg gctttattat
     3181 tgacagcgtt aaacgggact ttcagtcccg ttaccagacc agttttttag gcgcggcatg
     3241 gctgatctta cagccgatcg ccatgatttc cgtctataca ttaatttttt cagagttaat
     3301 gcgtgcccgc ctggcgggca tggatggtcc ttttgcctac agtatctacc tctgttccgg
     3361 ggtgttaacc tgggggctgt ttacggaaac gctcggcaat ctggtcaacg tttttctgac
     3421 caacgccaac attcttaaaa agcttagctt tccgcggatc tgtttaccga tcattgtcac
     3481 cgcctcggcg ttcattaact tcctgatcat ttttggtctg tttgtactgt ttctgatcgt
     3541 cacgggcaat ttccctggca tgattttctt tgaaatcatt ccggtgctga tcgtccagat
     3601 gctgttcacc ctcggcctcg ggatcattct cggggtactg aacgtttttg tccgcgatgt
     3661 cgggcagttc gttaatatcc tgcttcagtt ttggttctgg tttacgccaa ttgtctacgt
     3721 atccaaaacg ctgccggaat gggtttctgg tctgttggcg tataacccga tggcgactat
     3781 tatcggctca taccagaacg tgatgctcta tcaccagagc cctaactggc tggctctgct
     3841 tcctgttgcg gtgctgtccg tcattctgtt tttatttgct tggcgtttat ttaaaaaaca
     3901 tgccgctgat attgtggacg agatttaatc atgagtatca aagttcagca cgtcggcaag
     3961 gcgtataaat attatccatc caaatggagc cgggtcattg agaaattgct gccgggggat
     4021 aagccgcggc acagcaaaaa atgggtattg aaagatatca atttcagcat tgaacctggg
     4081 gaagcggtcg gcattgttgg ggtgaacggc gcaggtaaaa gtacgttact gaagctgctg
     4141 actggcacca ctcagcccac caaaggtagc attgaaatcc aggggcgcgt cgccgcgctg
     4201 ctggagctgg gcatgggctt ccatcctgac tttaccggcc ggcagaacgt gtatatgtcc
     4261 gggctgatga tgggcctggg ccgggaagag atcgagcgct taatgccgga gatcgaagcc
     4321 ttcgccgata tcggcgacta cattgaagag cccgtacgca tctattccag cgggatgcaa
     4381 atgcgcctgg cgttcgctgt cgccacggcc tcccggccgg atattctgat cgtcgatgaa
     4441 gcgctctccg tgggtgactc ccgttttcag gcgaaatgct atgctcgcat cgcggacttc
     4501 aaagagcagg gcaccacgct gctgctggtt tcccacagcg ccggggatat cgtcaagcac
     4561 tgcgaccgcg ccattttcct caaaaatggt gacatctgca tggacggcac cgcccgcgac
     4621 gtaaccaacc gttacctgga tgagctgttt ggcaaaccgg ataaagacag cgcgacaaaa
     4681 agcgcaacgg ctatctcgtc agccagtggc gaaagccaga tgtctctcga tgagattgaa
     4741 gatgtgtacc acacgcgccc aggctaccgt ccggaagaat atcgctgggg gcagggtggc
     4801 gcgaaaatca tcgattatca tatccagagc gccggggttg attttcctcc ctcactgacg
     4861 ggcaatcagc agaccgattt tctgatgaag gtcgtgtttg aatacgattt tgattgcgtg
     4921 gtgccaggca tcctgattaa gaccctcgat ggcttattcc tctacggaac caactctttc
     4981 ctcgcctctg aagggcggga aaatatttcg gtttcccgcg gcgatgttcg ggtctttaaa
     5041 tttagccttc cggttgattt aaatagtggc gattacctgc tgtcatttgg tatctctgcc
     5101 ggcaacccgc agacggatat gacgccgctg gacagacgtt acgactccat tattttacat
     5161 gtgaccaaga gcatggattt ctggggtgtc atcgatctta agtcgtcctt tactagctac
     5221 caatgacgtt gagaaaaacg attccatgac taaagactta aacacgctgg ttagcgaatt
     5281 acctgagatt tatcaaacta tttttggcca tcccgaatgg gatggcgacg ccgctcgtga
     5341 ttgtaatcaa cgcctcgatt tgattaccga acagtacgat aacttatccc gtgcgctggg
     5401 gcgcccgctt aacgttctcg atctgggctg cgcgcagggc ttttttagcc tgagtctggc
     5461 aagcaaaggc gcgactatcg ttggtatcga tttccagcag gaaaatatta acgtgtgccg
     5521 cgcgctggcc gaagagaacc ccgattttgc cgccgagttc cgggtaggca gaattgaaga
     5581 ggtgatcgcc gcgctggaag agggcgagtt tgatctggcc attggcttaa gcgtgtttca
     5641 ccatattgtt caccttcatg gtatcgatga agttaaacgt ctgctctcgc gcctggccga
     5701 tgtcacccag gcggtgatcc ttgagctggc ggtgaaagaa gaaccgcttt actggggcgt
     5761 ttctcagccg gacgacccgc gcgaactgat tgagcagtgc gcgttttatc gcctgatcgg
     5821 tgagtttgac acccatttat cccccgtacc gcgcccgatg tacctggtca gcaaccaccg
     5881 ggtgctgatc aacgatttta atcagccgtt ccagcactgg cagaatcagc cctatgccgg
     5941 ggccggcctg gcgcataagc gcagccgtcg ctacttcttt ggcgaagatt atgtatgcaa
     6001 gtttttctat tacgacatgc cccacggcat cctgacggcg gaagagagcc agcgtaataa
     6061 acatgaactg cataatgaaa taaagttcct gacgcaaccg ccagcgggct ttgacgcgcc
     6121 ggcagtactg gcccacggcg aaaatgctca gtctggctgg ctggtgatgg aaaaactccc
     6181 gggtcgtttg ctgagcgata tgctggcagc cggggaggag atcgatcgtg aaaaaatcct
     6241 cggtagcttg cttcgttcct tagcggcgct ggagaaacaa gggttctggc atgacgatgt
     6301 acgcccgtgg aacgtgatgg tcgatgcccg acagcatgcc cgcttaatcg acttcggctc
     6361 tatcgttacc acgccgcagg actgcagctg gccgacgaac cttgtccagt cgttcttcgt
     6421 gtttgtgaat gagctgtttg ccgaaaacaa aagctggaac ggtttctggc gctcggcgcc
     6481 tgttcatccg tttaatctgc ctcagccgtg gtctaactgg ctgtatgcgg tctggcagga
     6541 gccggttgaa cgctggaact ttgccctgct gttggcgctg ttcgagaaga aagcgaagct
     6601 gccgtcagct gaacagcagc gcggggcgac ggaacagtgg atcatcgctc aggagacggt
     6661 gctgctggag ctccagtccc gggggcgaaa tgaaagcgcc ggctcagaag cgttgcgcgg
     6721 ccaaatccat accctggagc agcagatggc tcagctgcag tccgctcagg atgcgttcgt
     6781 cgagaaagct caacagccag tggaagtcag ccatgaactc acctggcttg gtgagaatat
     6841 ggagcaactg gcggcgctgt tgcagacggc gcaagcgcac gcccaggctg acgttcagcc
     6901 cgagttgccc cctgaaaccg ctgagctact gcagcgactg gaagccgcta accgggagat
     6961 ccaccattta agcaacgaaa accaacagct tcgccaggaa attgaaaaaa tccatcgcag
     7021 ccgttcctgg agaatgacca agggttatcg ttatctgggc ctgcagatcc atttgctgag
     7081 gcagtatggt ttcgtgcagc ggtgtaaaca ttttattaag cgcgtcctgc ggtttgtttt
     7141 ctcatttatg agaaaacatc cgcaggtaaa acataccgcg gtaaatggtt tacataagct
     7201 ggggctttat cagccggcct atcgtttgta tcgtcgtatg aatccactgc cgcacagcca
     7261 gtatcaggct gatgcgcaga ttttatcgca gactgaactg caagtgatgc atccggagct
     7321 tttacctccg gaggtttatg aaatttatct caaactaacg aaaaataaat aaggagatta
     7381 acttgcatat tttgattgat gtccagggtt accagtcgga aagtaaagtc cgcggtattg
     7441 gtcgcagtac cctggccatg agccgcgcca ttattgaaaa tgccggtgag catcgcgtca
     7501 gtattttgat caatggtatg tatcccattg ataatattaa tgatgttaaa atggcctatc
     7561 gggacctgct gacggatgaa gatatgttca tcttctcagc cgtcgcgcca acagcctatc
     7621 gtcatattga aaaccatggc cgcagtaaag cggcccaggc ggcccgcgat atcgctatcg
     7681 ccaatatcgc gcctgatatc gtctatgtta ttagcttctt cgaggggcat agcgatagtt
     7741 ataccgtgtc tattccggcg gataatgtgc cgtggaaaac ggtgtgcgta tgccacgatc
     7801 tgatcccgct gttaaataaa gagcgttatc tcggcgatcc gaatttccgt gagttttata
     7861 tgaataagct ggcggaattt gagcgggcag acgcgatctt cgccatttcg cagtcggcag
     7921 ctcaggaagt gatcgaatat accgacatcg ccagcgatcg ggtactgaac atctcctcgg
     7981 cggtgggcga agagtttgcg gtgatcgatt attccgctga gcgtattcag tctttaaaag
     8041 acaaatacag cctgccggat gagtttatct tgtcgctggc gatgatcgag ccgcggaaaa
     8101 atattgaagc gcttattcac gcctacagct tgctgcctgc cgagctgcag cagcgctatc
     8161 cgatggtgct ggcgtataaa gtgcagccag aacaactgga gcggatcctg cgtctggcgg
     8221 aaagctatgg tttgtcacgc agccagctta tctttactgg gttcctgacc gacgacgatc
     8281 tgattgccct gtacaacctg tgcaaactgt ttgtgttccc gtcgctgcat gaaggtttcg
     8341 gcctgccgcc gctggaagcg atgcgctgcg gggcggcgac cttaggttca aacattacca
     8401 gcctgccgga agtcattggc tgggaagatg ccatgttcaa tccgcatgat gtgcaggaca
     8461 ttcgccgggt catggagaag gcgctgaccg atgaggcgtt ttatcgtgag ctgaaggcgc
     8521 atgctctcgc gcagtcggcc aaattctcgt gggccaatac cgcccatctg gcgatcgagg
     8581 gtttcactcg tctgctgcag tcgtcccaag agacggatgc cgggcaggcg gaaagcgtga
     8641 ccgcctcccg ccttcagatg atgcagaaaa tcgatgcgct gagcgaagtc gaccgtcttg
     8701 gtctggcatg ggcggttgcg cgcaatagct ttaagcgcca tacccgcaag ctgctggtgg
     8761 acatttcggt tctggcgcac gatgacgcga agaccgggat ccagcgcgtc tcgcgcagta
     8821 tcctcagcga attactgaaa tctggcgtgc cgggttacga ggtttccgcc gtctactaca
     8881 cccctggcga gtgttatcgc tacgccaacc aatatctgtc cagtcatttc ccgggcgaat
     8941 ttggtgctga cgaaccggtg ctgttcagca aagacgatgt gcttattgcc accgatctga
     9001 ccgcgcatct cttcccggag ctggtgacgc aaattgacag catgcgcgcc gccggggcgt
     9061 tcgcctgctt cgtggtgcat gatattctgc cattacgccg tccggagtgg agtatcgaag
     9121 gcattcagcg tgatttcccg atctggctgt cctgcctcgc agagcacgcc gaccggctga
     9181 tctgcgtctc tgccagcgtc gccgaggatg tgaaagcgtg gattgcggaa aatcgccatt
     9241 gggtgaaacc gaacccgctg cagaccgtca gcaactttca tctgggagcc gacctcgatg
     9301 ccagcgtacc gtccactggc atgccggata atgcacaggc gctgttagca gcgatggctg
     9361 cagctccgtc atttatcatg gtgggtacca tggagccgcg caaaggccac gcccagacgc
     9421 tggccgcctt cgaggaactg tggcgcgagg gcaaagacta caacttgttt atcgtcggca
     9481 aacagggctg gaacgttgac agcttgtgcg aaaaattacg ccatcatccg cagctgaaca
     9541 aaaagctctt ctggctgcag aatatcagcg acgagttttt ggccgagcta tacgctcgtt
     9601 cacgcgcgct gatctttgcc tcgcagggag aaggctttgg cctgccgttg attgaagcgg
     9661 cgcagaaaaa gctgccggtg attattcgcg acattccggt gtttaaagag attgctcagg
     9721 aacatgcctg gtatttctcc ggtgaagcgc cgtccgatat cgcgaaggcg gtagaagagt
     9781 ggttagccct gtacgagcaa aacgcgcatc ctcgttccga aaatatcaac tggttaacct
     9841 ggaaacagag cgcggaattt ctcctgaaaa acctgccgat tatcgcgcca gccgcgaagc
     9901 aataatcgct atcaacaggg ctactgcggt ggcccttgtg atgagttcgt tatgaaaatt
     9961 atttttgcta ctgagccaat taaataccca ttaacgggca tcggtcggta ttccctggag
    10021 ctggttaagc ggctggcggt tgcccgtgaa atcgaagagc tgaagctgtt tcacggcgcg
    10081 tcgtttatcg accagatccc ccaggtggag aataaaagcg atagcaaagc cagcaatcat
    10141 ggtcgtctgt tggcgtttct gcgccgccag ccgctgctga ttgaggcgta tcgcctgctg
    10201 catccgcggc gccaggcgtg ggcattgcgc gattacaaag actacattta ccatggtccc
    10261 aatttctatc tgccgcatcg cctggaacgc gccgtgacca cgtttcatga catctccatt
    10321 tttacctgcc cggaatatca tccaaaagat cgggttcgct atatggagaa gtccctgcac
    10381 gagagcctgg attcggcgaa gctgatcctg accgtctccg acttctcgcg cagtgaaatc
    10441 atccgtctgt tcaactatcc ggcggatcgg atcgtcacca ccaagctggc ctgcagcagc
    10501 gactatattc cgcgcagccc ggcggagtgc ctgccggtcc tgcagaaata tcagctggcg
    10561 tggcaggggt atgcgttata tatcggcacc atggagccgc gtaaaaatat ccgcggtctg
    10621 ctgcaggcct atcagctgct gccgatggag acccgcatgc gctacccgct gatcctcagc
    10681 ggttatcgcg gatgggaaga cgatgtgctg tggcagttag tcgagcgcgg tacgcgtgaa
    10741 gggtggatcc gttacctggg ctatgtcccg gatgaagacc tgccttatct gtacgcggcg
    10801 gccagaacct ttgtttatcc ctccttctat gagggattcg gtttacctat tcttgaagcg
    10861 atgtcttgcg gtgtgccggt agtatgttcc aatgtcactt ctttgcctga ggtggttggc
    10921 gatgccggcc tcgttgccga tcctaatgat gtagacgcga ttagcgcaca tattttgcag
    10981 agcctgcagg atgatagctg gcgggaaatc gccaccgcgc gcggtcttgc ccaggcgaaa
    11041 cagttttcgt gggagaactg tacgacccag accattaacg cctataaatt actctaaggg
    11101 tgtcagttga gagttctaca cgtctataag acctactatc ccgataccta cggcggtatt
    11161 gagcaggtca tttatcagct aagtcagggc tgcgcccgcc ggggaatcgc agccgatgtt
    11221 tttactttta gcccggacaa agagacaggt cctgtcgcct acgaagacca tcgggtcatt
    11281 tataataagc agctttttga aattgcctcc acgccgtttt cgttgaaagc gttaaagcgt
    11341 tttaagcaga ttaaagatga ttacgacatc atcaactacc attttccgtt tcctttcatg
    11401 gatatgttgc atctctcggc gcggcctgac gcaagaacgg tggtgaccta tcactcggat
    11461 attgtgaaac aaaaacggtt aatgaagttg taccagccgc tgcaggagcg attcctcgcc
    11521 agcgtagact gcatcgtcgc ctcgtcgccc aactacgtgg cctccagcca gaccctgaaa
    11581 aaatatcagg ataaaaccgt ggtgatcccg tttggtctgg agcagcatga cgtgcagcac
    11641 gattcgcagc gggtggcgca ctggcgggaa accgtcggcg ataacttctt cctcttcgtc
    11701 ggcgctttcc gctactacaa agggctgcac attctgctgg atgccgccga gcgtagccgg
    11761 ctgccggtgg tgatcgtcgg gggcgggccg ctggaggcgg aagtgcggcg tgaggcgcag
    11821 caacgcgggc tgagcaatgt ggtgtttacc ggcatgctca acgacgaaga taagtacatt
    11881 ctcttccagc tctgccgggg cgtggtattc ccctcgcatc tgcgctctga ggcgtttggc
    11941 attacgttat tggaaggcgc acgctttgca aggccgctga tctcttgcga gatcggtaca
    12001 ggtacctctt tcattaacca ggacaaagtg agtggttgcg tgattccgcc gaatgatagc
    12061 caggcgctgg tggaggcgat gaatgagctc tggaataacg aggaaacctc caaccgctat
    12121 ggcgaaaact cgcgtcgtcg ttttgaagag atgtttactg ccgaccatat gattgacgcc
    12181 tatgtcaatc tctacactac attgctggaa agcaaatcct gagagctgct acccccgccg
    12241 ttgctgcggg ggccaacgcg ttaatgccga ttcttcagat tatcaatcac ttctccgaga
    12301 tccagcccct gatcctgcaa cagcaccagc aggtgataca tcaaatcaga tgcctcgttg
    12361 gtcagctcaa agcggtcatt taccgtcgcg gccagtgcgg tttccacgcc ttcttcgccc
    12421 actttctgcg caatgcgttt ggtgccgctg gcatacagtt tggctgtgta agaggtttcc
    12481 gggtcggcag atttgcgttc ggcgagcagt tgttccagtt gatacaggaa cagccactgg
    12541 tgagcggtgt cgccgaagca gctgctggtg cctttgtggc aggtcgggcc gatgggatta
    12601 gccagcacta ataacgtgtc gttgtcgcag tccggggcaa tactcaccac attgaggaaa
    12661 ttgcccgacg tttcgccttt ggtccacagt cgctgtttag tgcgcgagaa gaaggtgact
    12721 ttgccgcttt cgatggtctt gtctaaagct tccgggttca tatagcccag cattaacact
    12781 tcgccggata ccgcatgttg cacaattacc ggcatcagtc cgtcggtttt ttcccagtcc
    12841 agttcgcgac gttgtagttc tgttaacata tcctgatctc cacgccctgt gttgccaggt
    12901 acgcttttaa ttcaccaata ttgattattt gtttgtggaa tacggaagct gccagcgcgc
    12961 cgtcaacgtc ggcatcacgg aaggcttcga ggaagtgttc catggtgccc gcgccaccgg
    13021 aggcaatcag cgggacgtgg caaacttcac gcactttttt cagttgttcg aggtcgtaac
    13081 cgttacgcac gccgtcctga ttcatcatat tgaggacgat ttctccggca ccgcgttttt
    13141 gcacttcctg tacccagtcg agcgtttccc actgagtgac gcgggtgcgg ctttcgtcgc
    13201 cggtatattg attcacatga tatttaccgg tttcagcgtc gtaccaggta tcaataccga
    13261 ccacaataca ctgcacgcca aagcgatccg ccaggcgagt aattaacgtc ggatcc
//

</pre>