LOCUS       KJ778812               15318 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain H 53 serotype O88:H25 O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ778812
VERSION     KJ778812.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15318)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15318)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Swimley,M.,
            Baranzoni,G.M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="H 53"
                     /serotype="O88:H25"
                     /db_xref="taxon:562"
     misc_feature    complement(1..15318)
                     /note="O-antigen gene cluster"
     CDS             660..1796
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DDE domain protein"
                     /protein_id="AIG62887.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDFIAIDGKTLRHSYYKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKRHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFS
                     GIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            1860..2453
                     /gene="vatD"
     CDS             1860..2453
                     /gene="vatD"
                     /codon_start=1
                     /transl_table=11
                     /product="streptogramin A acetyltransferase"
                     /protein_id="AJE24507.1"
                     /translation="MKQALSRMKIKLLSYFYKRKNRSTQFGRYTVLFCRANITNSIVG
                     DYTYFAGTASVNNCEIGKFCSIAEGVKIGLGKHPVDFLSTHPVFYSENTCFPYRLKNY
                     RVNEKVIESITESERVIIGNDVWIGVNAIVMDGVTIGDGAVIGAGAVVTKDVQPYTIV
                     GGVPARVIRTRQNINCSWWEFDVDTLVDFSSNFKKRL"
     gene            2453..3037
                     /gene="lacA"
     CDS             2453..3037
                     /gene="lacA"
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="AIG62885.1"
                     /translation="MYELMLKVKRKIATLLSSFIFRVSFKNFGTKSSIFKPDILQGVK
                     YIEVGDRVVIQPGLWALALKIDNNEPILNIGNNVYIGRFLHIVSVRRVVIEKDVLIAD
                     KVYISDNLHEYKDIHIPVSQQPVVFKGDVVIKEGVWIGENVSIIGACIGRNSVVAANS
                     VVTKDIPDYSVCAGVPAKVIKKYCFERNEWISVS"
     gene            3070..4152
                     /gene="rmlB"
     CDS             3070..4152
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="AIG62884.1"
                     /translation="MKILVTGGAGFIGSALTRYIINSTNDSVVNVDKLTYAGNLHSLK
                     DIDNCDRYVFVHADICDAKALDAILSTHKPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARKYWQTLEGEKKQGFRFHHISTDEVYGDLPHPDDWDNKNSPPLFTENTA
                     YAPSSPYSASKASSDHLVRAWHRTYNLPTIVTNCSNNYGPYHFPEKLIPLVILNALQD
                     KSLPIYGKGDQIRDWLYVEDHARALYTVITKGIVGETYNIGGYNEKKNIEVVETICDI
                     LDEIKPKNTSYREQIIYVADRPGHDRRYAIDASKITLDLGWKPQETFESGIKKTIHWY
                     LDNQEWVQNVMSGAYQDWINKNYGQL"
     gene            4185..5060
                     /gene="rmlA"
     CDS             4185..5060
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="AIG62883.1"
                     /translation="MSKRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFEQLLGNGEKWGLNIEYKVQESPDGLAQAFIIGEEFIGN
                     DSCALVLGDNVFYGHDLPKELEIAMNQEKGATVFAYHVKDPERYGVVDFDENGKALSL
                     EEKPLKPKSNYAVTGLYFYDNEVIEMAKQLKPSARGELEITDINRLYLEKNSLSVAIM
                     GRGYAWLDTGTHESLIEAGNFIQTIESRQGLKVSCPEEIAFRKGFIDKKQLAKLAKEL
                     NKNDYGKYLQHLAKD"
     gene            5087..5647
                     /gene="rmlC"
     CDS             5087..5647
                     /gene="rmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="AIG62882.1"
                     /translation="MKIIETPLKDAVIIEPKIFGDERGYFFEVYQKKRYQELGFALEF
                     VQDNRSKSTKNVLRGLHFQKTKPQGKLVSVTQGAVFDVAVDLRPESLTFGQHHSVILS
                     EENFLQFYIPPGFAHGFCVLSDTASFQYKCTDYYDPTDESGLIWNDPDLGIEWPVSHP
                     VVSAKDCFLPSLASIKEQILKGWKNV"
     CDS             5640..6464
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="AIG62881.1"
                     /translation="MSKYTIIGGNGFIGSHIVTLLKEQGHNVVVPDRNSCNSLNGELG
                     KIIYCAGNGDCAESYFSVLEANTVLLAKLLKNAQFEKLVYISSTRVYMNHHSANETDD
                     LLVTAGDNRKLFNLTKLVAEELCIKSERDVTVVRPSNVYGLALNSKLFLPSIIRHAIN
                     NGEINMFVAPEYAKDYVSVNDVAKACVWLAEKQNTNKEIYNVASGFNVTAKQITDVIE
                     KETACKVNWHDGNFPREEFPVTNINKLTNEIPDYKPQHVLDDLKMMIASYKEELAR"
     gene            6467..7924
                     /gene="wzx"
     CDS             6467..7924
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AIG62880.1"
                     /translation="MSGFKRLLKNSLSNIINGFSNVILGIVISPVLLHNLSKVDFSIW
                     SLTIQVGVLIGIFGIGLQVTVGRFVALHLNNQQKLQKTMHQSLFFVLLLSLVCMGGIF
                     FLAKFFFSFFPEVSETSTNAKHILILIGGSFVINNMFSPFVGYFTGIERNDITAGINI
                     LFKVILGTSILLLVHHGLEVIAWIYFAINTFNQLAFYILYKLKNRTQKIKLSLDKNLF
                     RTIVVFFSGLLVWNIAQFLISGIGTFIVGKYSFQELAGFAVLMTLVNAGVGILGAMIN
                     PIIQPMIRMHNLENNHHVELLVNKLIFIFSMIVFIGVFIAWFASVYILGVWLGYQQAT
                     NLHIMFSLLLTAYLIRMIAAPYGLMLVAYGKQLSIAWLPVLEGVLNFALSIFFVRIYG
                     AIGIAYSTFISGVLIMAVYACKYQAEAEYKSKLIFISFLIIPMMIAICLISLVMTQSE
                     IIHCVIYGIQVAFIIVFIIFLIKQVKSIKNLLNQY"
     CDS             7943..8839
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="AIG62879.1"
                     /translation="MKKVGLVTVLFNSPGVLPEFYSSVEKQNYQNKHLYIVDNSTNQD
                     SFNLTQEILLGTDIKYTYINNEGNNVGVAHANNQGIEQALKDGCEYILLTNNDLFFQQ
                     EDTISKMISVAEGRQCKIVSPVILSYPEKKVWYAGAFFNKKKATAPHIFEGVNYNDIC
                     AHIPKECDYAPTCFLLVHASVFTTIGMMDSRYFAYYDDTDFLYRANIAGTRVTIINEP
                     LVFHKVSTSTGGGLSVFGAYYLTRNRIFFARKNIETPYCIISIFFTIATRLVYPLFHK
                     TPYKVYKAFVKGIIDGLMLKNK"
     gene            8853..10127
                     /gene="wzy"
     CDS             8853..10127
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AIG62878.1"
                     /translation="MNEISSSTLVNNYNLSTTFIVKSLVGLLLINSLFSGVDFYQIGL
                     MKGLSSIADFGLKIILVFVLLVLFKIRSFNLVKIFFFAFMLLIGAALGAFYHGAEYDK
                     TFALLLNMILWFVIFSYAKELEHNFDIYRFSVKISSLFLGITLILYIMAKFGFHVKVL
                     GGAWLYDENFRFAGTFSEPSLNGYYYGLMFLMVMLSNQKYRLILAFVFAITTYISGAK
                     FSFLIIPVLLGLVYFFKVKGFYNYTYQLIPLSFIVLFVSLAFIYYDLFALIAAHLSNS
                     ETMTYVTRLGFPIVSIWHLSDYPLGSGVYGFKSTLSPFIQNYCQALSGLDVNCNEMRS
                     YISSDDPSAYESFAPKDVLSFIILSFGLPGLLVFVLSISLLSARLKENKNYFIILMYI
                     VASMLFTLPFRFILFYSFFMFQCYIYRMPSNK"
     CDS             10124..11179
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="AIG62877.1"
                     /translation="MIYINARFLTQRKTGVQQYALVLCRELVKHIPDICFLTPKTDLI
                     DENWRDEFNILQIGNRNGHFWEQIELPRYLRSVGNPLLLCFTGLSPAFYKNCYFTIHD
                     MSLYAHPKWFRLLYRAVYKVNYAIQARFARGIFTVSDFSKSEIEKYLAVDSDKITVLR
                     NSVDTSINIKNNIENKAVRRKEILLVGTLEPRKNIEFIINSFLNSQIKGYKLIVVGGL
                     GAAFSKVTFSQHENIIFCGYLSDDDLELKYRQASVFVYPSLYEGFGLPPLEAMQRGCP
                     VMASDRASIPEVCGDAAYYFNPEDSSDFSSKLRELIENYDELSPVLICNGYRRLEQFD
                     VRDVASKLMDVISQRNI"
     gene            11180..12289
                     /gene="pimB"
     CDS             11180..12289
                     /gene="pimB"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose-dependent
                     alpha-(1-6)-phosphatidylinositol monomannoside
                     mannosyltransferase"
                     /protein_id="AIG62876.1"
                     /translation="MKVLQFSKFYPPVHGGIEQVAFDISEGITKSNGQPVDVLCVDPI
                     GERKDDGKFRYKVYRQKLFAQLFSTPLSFSLILRWRAIRNNYDVIHVHLPNPMAVLAL
                     FLFPPKGKIVLHWHSDIVKQKKLLLLFAPLQKWILDKCTCIIVTSPVYGQSSPTLQPY
                     QDKIVCIPIGVDTHVMPLNDELEKSIKKRYQNKKIVFSLGRLVYYKGMEYLIDAAKEL
                     PPDYIVLIGGTGPLIDTLKQKVANDGLSDKVVLLGSINYSDLASYYKSCDVFCLPSIH
                     ESEAFGVVQLEAMSFSRPLVSTNIPRSGVAWVNQHNETGIVVEPNDAKALAKGIVSVV
                     QNSATFSKGAKARFDTMFTKELMVKNIINLYSSLK"
     gene            12298..13725
                     /gene="manC"
     CDS             12298..13725
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase 1"
                     /protein_id="AIG62875.1"
                     /translation="MPLFPVVMAGGSGSRLWPLSRVLYPKQFLCLEGELTMLQATIHR
                     LQGIVCESPVVICNEQHRFIVAEQLRQMNKLTENIILEPIGRNTAPAIALAALAATRA
                     QPEGKALMLVLAADHVIKNEEAFRDSVRKALPYAENGKLVTFGIVPNKAETGYGYIRR
                     GVPCDGVEHADAFDVAQFVEKPNLETAEAYVSCGEYYWNSGMFLFRADRYLEELKKYR
                     PDILEACEKAMSAVEPDLNFIRVDEDAFRACPDESVDYAVMEQTSDAVVVPMDAGWSD
                     VGSWSSLWDISHKTPEGNVVTGDVLSCNTKNSFLYSESGLLTTVGVKDLVVVQTKDAV
                     LIANRNAVQDVKKIVESIKANGRHEHHTHREVYRPWGKYDSIDTGSRYQVKRLTVKPG
                     ESISLQMHHHRAEHWIVVAGAAQVTINEQTKLLGENESIYIPVGSTHCLENPGKIPLE
                     LIEIRSGSYLGEDDIVRFADKYGRV"
     gene            13744..15114
                     /gene="algC"
     CDS             13744..15114
                     /gene="algC"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="AIG62874.1"
                     /translation="MEKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTATGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LALLNK"
ORIGIN      
        1 tgctgatgac aggtgacagc tacgactgcg gtaaaaaaat gggttatatg caggcgtttg
       61 tgaagtatgg actacgcaac ctcaaagaag gggcgaagtt ccgtaaaggg attgagaagc
      121 tgttaagcga ataatgaaaa tctgaccgga tgtaacggtt gataagaaaa ttataacggc
      181 agtgaagatt cgtggcgaaa gtaatttgtt gcgaatattc ctgccgttgt tttatataaa
      241 caatcaggat aacaacgagt tagcaatagg attttagtca aagttttcca ggattttcct
      301 tgtttccaga gcggattggt aagacaatta gcgtttgaat ttttcgggtt tagcgcgagt
      361 gggtaacgct cgtcacatcg taggcatgca tgcagtgctc tggtagctgt aaagccaggg
      421 gcggtagcgt gtctagggga aggtcatcgc gtgcgttaag cacaaaaaga aaaaatgaac
      481 gtttgcatgt aataagcata agtttgtgaa tagatcaggg aaagatcatc agaagttcac
      541 tttttgtact aaataattcg cattttatgt ttaaaaattg agatattcct tattacctaa
      601 agctgttttt tattgcttat acatgatcaa atactcctta cataattaag gagaacaaaa
      661 tggaacttaa aaagttgatg gaacatattt ctattatccc cgattacaga caagcctgga
      721 aagtggaaca taaattatcg gatattctac tgttgactat ttgcgccgtt atttctggtg
      781 cagaaggttg ggaagatata gaggattttg gggaaacaca tctcgatttt ttgaagcaat
      841 atggtgattt tgaaaatggt attcctgttc acgataccat tgccagagtt gtatcctgta
      901 tcagtcctgc aaaatttcac gagtgcttta ttaactggat gcgtgactgc cattcttcag
      961 atgataaaga cttcattgca attgatggaa aaacgctccg acactcttat tacaagagtc
     1021 gccgcagggg agcgattcat gtcattagtg cgttctcaac aatgcacagt ctggtcatcg
     1081 gacagatcaa gacggatgag aaatctaatg agattacagc tatcccagaa cttcttaaca
     1141 tgctggatat taaaggaaaa atcatcacaa ctgatgcgat gggttgccag aaagatattg
     1201 cagagaagat acaaaaacag ggaggtgatt atttattcgc ggtaaaagga aaccaggggc
     1261 ggctaaataa agcctttgag gaaaaatttc cgctgaaaga attaaataat ccagagcatg
     1321 acagttacgc aattagtgaa aagcgtcacg gcagagaaga aatccgtctt catattgttt
     1381 gcgatgtccc tgatgaactt attgatttca cgtttgaatg gaaagggctg aagaaattat
     1441 gcgtggcagt ctcctttcgg tccataatag cagaacaaaa gaaagagcca gaaatgacgg
     1501 tcagatacta tatcagttcc gctgatttaa ccgcagagaa gttcgccaca gcaatccgaa
     1561 accactggca cgtggagaat aagctgcact ggcgtctgga cgtggtaatg aatgaagacg
     1621 actgcaaaat aagaagagga aatgcagcag aattattttc agggatacgg cacattgcta
     1681 ttaatatttt gacgaatgat aaggtattca aggcagggtt aagacgtaag atgcgaaaag
     1741 cagccatgga cagaaactat ctggcgtcag tccttgcggg gagcgggctt tcgtaatctt
     1801 gccttaagta gcgtagattt ttccaaatca cattgatatt acaaagagtc ggtgacgata
     1861 tgaagcaagc tttgtcgaga atgaaaataa aattgttatc atatttttat aaaagaaaaa
     1921 atcggtctac acaattcggt cgctacactg ttttattctg tcgtgcaaat ataactaatt
     1981 ctatagtagg tgattatacg tatttcgcag gcaccgcctc cgttaataat tgtgaaattg
     2041 gtaaattttg ttctattgca gaaggcgtta aaataggcct tggaaaacac ccagttgatt
     2101 ttctttcaac acatccggta ttttactcgg aaaatacctg cttcccttat agattgaaaa
     2161 attatagagt taatgaaaaa gtgattgaat caatcaccga atcagagaga gtcatcatag
     2221 gcaatgatgt ttggattggc gtaaatgcca tcgtaatgga tggtgtgaca attggtgatg
     2281 gagcagttat tggagctggg gcggttgtta ccaaagatgt tcaaccttat actatcgtag
     2341 gtggtgttcc tgcgagagtc attaggacca gacaaaatat aaattgttct tggtgggaat
     2401 ttgatgtaga tacccttgta gatttttcct caaattttaa aaagaggttg taatgtacga
     2461 gttaatgttg aaggtcaaac gtaaaatagc tactcttctt tcttcattta tatttcgcgt
     2521 ttcatttaaa aatttcggaa caaaaagtag tatttttaaa cctgatatat tacaaggggt
     2581 gaaatatata gaagttggcg atagagtagt aattcaaccg ggattatggg ctttagcatt
     2641 gaaaattgat aacaatgaac cgatcctgaa tataggaaat aacgtttata ttggtcgctt
     2701 cttacatatt gtaagtgtgc gccgtgtagt tatcgaaaag gatgttctca tcgccgataa
     2761 ggtatatata tcagacaacc ttcatgagta taaagatatt catattccag taagccagca
     2821 gcctgttgta ttcaaaggtg atgtggttat taaggaaggc gtctggatag gggaaaatgt
     2881 ttccatcatt ggtgcttgca ttggcagaaa tagtgtggta gcggcaaact ctgtagtgac
     2941 gaaggatata cctgattatt ctgtttgtgc aggtgtacct gcaaaagtta tcaaaaaata
     3001 ctgtttcgaa cgaaatgaat ggataagtgt ttcttaatgt ataccatttt tacttatttg
     3061 agataaaaaa tgaaaatatt agttactggt ggtgccgggt tcatcggttc ggcattaacg
     3121 cgatacatta ttaactcaac aaatgactct gttgtgaatg ttgataagtt aacctacgca
     3181 ggtaatcttc attctttaaa agacattgat aattgcgatc gttatgtttt tgttcatgct
     3241 gatatttgcg atgcgaaagc attagacgca atcttatcaa cacataaacc tgatgcagta
     3301 atgcatttgg cagcagaaag tcatgttgac cgttcaatca ccggccctgc tgcgtttatt
     3361 gaaacgaata ttgttggaac atacgttttg cttgaagcag ccagaaaata ctggcaaacc
     3421 ctagaggggg aaaagaaaca gggattccgt ttccatcaca tttctactga tgaagtatat
     3481 ggtgatctcc ctcatccgga tgactgggat aataaaaata gtccaccact gtttactgag
     3541 aataccgcat atgctcctag cagtccatac tctgcctcta aagcatcaag cgatcatttg
     3601 gttcgcgcgt ggcatcgtac ttataatctc ccaactattg tgactaactg ttcaaacaat
     3661 tatggtcctt accatttccc agaaaagtta attccgctgg tgatccttaa tgcgttgcaa
     3721 gacaaatctt tgccaattta tggcaaaggc gatcaaatcc gtgattggtt atacgttgag
     3781 gatcatgctc gcgcactcta taccgttatc acgaaaggta tcgtaggtga aacttacaat
     3841 atcggcggtt ataatgagaa aaagaatatt gaagtggtag agactatttg cgatatcctt
     3901 gacgaaataa agccgaaaaa tacctcgtat cgcgaacaaa tcatttatgt tgccgatcgc
     3961 ccaggtcacg atcgccgcta tgccattgat gccagtaaaa tcactctgga cctcggttgg
     4021 aaacctcaag agacctttga atctggtatt aagaaaacta ttcattggta tcttgataac
     4081 caggagtggg tccagaatgt tatgagcggt gcctatcagg actggattaa caaaaattac
     4141 gggcaacttt aatcttttca actctgtcat aagaagcatt gaacatgtca aaaagaaaag
     4201 gaattatttt agccggtggt tcgggtactc gcctttaccc cgtgacgatg gcggttagta
     4261 aacagctgct gccaatctat gataaaccga tgatttatta tccattaagc acgttgatgc
     4321 ttgcgggtat tcgggatatt ctcatcatta gcacacctca ggatacccct cgctttgagc
     4381 aattacttgg taacggtgaa aaatgggggc tgaacatcga atataaagtc caggaaagtc
     4441 ctgatggtct tgcccaggca tttataattg gtgaagaatt tatcggtaac gattcttgtg
     4501 ctttagtcct cggcgacaat gttttctatg gtcacgattt gccaaaagaa cttgaaatag
     4561 caatgaatca ggaaaaaggt gctacggtct ttgcctacca tgttaaagac cctgaacgct
     4621 atggtgtggt agatttcgat gagaatggca aagcgctttc tttagaagag aaaccgctga
     4681 aaccaaaaag taattatgct gtgactgggc tctattttta tgataacgag gtcattgaga
     4741 tggctaaaca actgaaaccg tcagctcgcg gtgaattaga aatcactgat attaatcgcc
     4801 tctacctgga aaaaaactcg ctctcagttg ctattatggg ccgtggctat gcctggcttg
     4861 atacaggtac gcatgagagt cttattgagg caggtaactt tattcagacg atcgaatcac
     4921 gtcaaggact caaagtttct tgcccggaag agattgcttt ccgtaaaggt ttcattgata
     4981 agaaacagct tgcaaaatta gccaaagaac tcaataaaaa tgattacggt aaatatttgc
     5041 agcatttagc caaagattaa aaattaacat taattagtta acacatatga aaattataga
     5101 aactcctttg aaagatgctg ttattatcga gcctaaaatt tttggtgacg aacgaggcta
     5161 tttttttgaa gtctatcaga aaaaacgtta tcaggaattg ggattcgctc tggaatttgt
     5221 acaggataat cgttccaaat cgactaaaaa tgtcctgcga gggcttcatt ttcagaaaac
     5281 aaagcctcag ggaaaactag tttcagtaac ccagggggct gtttttgatg ttgccgttga
     5341 tctccgtcca gagtcactga catttggtca gcatcattca gttattctat ccgaagaaaa
     5401 ttttcttcag ttttatattc ctccaggttt cgctcatggt ttctgcgtat taagtgacac
     5461 tgctagtttc cagtacaaat gcactgatta ttatgatcca accgatgaaa gcggtcttat
     5521 ctggaacgat cccgatctgg gcatagaatg gcctgtttct catcctgtag tttctgctaa
     5581 agattgtttt ttaccttcac tagcaagtat caaagaacag attcttaaag gctggaaaaa
     5641 tgtctaagta tacaattatt ggcggtaacg gttttattgg ttcccatatc gttacattgc
     5701 taaaagaaca agggcataat gtcgttgttc ccgatcgtaa tagctgcaat tccctaaatg
     5761 gtgagttagg taaaattatt tattgtgctg gtaatggtga ttgtgcggaa agctatttct
     5821 ctgtgctgga agcaaataca gttttgttgg caaagttact gaagaatgca cagtttgaaa
     5881 agctggtata tatttcctca actcgagttt atatgaacca ccatagcgca aatgaaactg
     5941 atgatctgct agtaacagcg ggtgataaca ggaagctttt taacctgacc aaactggtgg
     6001 ctgaggagct gtgtattaag agcgaacgtg atgttactgt ggttcgacca agcaatgtct
     6061 atggtttagc tcttaatagt aaattatttc ttccgtctat tatccgtcat gccattaaca
     6121 atggtgaaat caacatgttt gttgcacctg aatatgctaa ggactatgtt tcagtcaatg
     6181 atgttgctaa agcatgtgtg tggctagctg aaaaacaaaa caccaacaaa gaaatatata
     6241 atgtcgcatc tggatttaac gtaacagcta aacaaatcac tgatgtcatc gaaaaagaaa
     6301 cggcttgtaa ggtaaactgg catgatggta atttccctcg tgaagagttt cctgttacta
     6361 acataaataa attaactaat gagattccgg attataaacc acaacatgtg cttgatgatt
     6421 tgaaaatgat gatagctagt tataaagaag aactggcgag ataaatatga gtggttttaa
     6481 gcggttactt aaaaactctt tgtctaatat aataaatgga ttttcaaatg taattcttgg
     6541 aatagtgatc tctccagttt tactgcataa tctttcaaag gtagattttt ctatttggtc
     6601 ccttacaatt caggttgggg ttttgatcgg tatttttggc attggtcttc aggttacagt
     6661 agggcgtttt gtcgcccttc atttaaataa tcaacagaaa cttcaaaaga caatgcatca
     6721 gtcattgttt tttgtgttac tgctcagttt ggtatgtatg gggggaatat tctttttagc
     6781 aaaattcttt ttctcatttt tccctgaagt atcagaaacc agtactaatg ctaagcacat
     6841 tcttatatta attggtggtt catttgttat taataatatg ttctctccct tcgttggcta
     6901 ttttactggt attgagcgta atgatataac agctggcata aatatattgt ttaaagtaat
     6961 tctgggtacg tcgattctgt tgcttgtcca ccatggtctt gaagttattg catggattta
     7021 ttttgcaata aacactttta atcaacttgc tttttatatt ttatataaat taaaaaacag
     7081 aactcaaaaa ataaagttat cactagataa aaatttattt cgaactatag ttgtcttttt
     7141 tagcggactc ttggtatgga atattgccca gttcttaatt tctggtatag gcacctttat
     7201 cgtcggtaaa tattcatttc aggagctcgc tggttttgcc gtattaatga cacttgttaa
     7261 tgctggtgtg ggaattttgg gagcaatgat caatcctata attcaaccta tgataaggat
     7321 gcacaatctg gagaataacc atcatgttga acttctggtc aacaaactga tcttcatttt
     7381 ctcaatgatt gtctttattg gtgtttttat tgcctggttt gcctctgtat atattcttgg
     7441 cgtctggttg ggatatcagc aagcaacaaa cctgcacatt atgttttctt tgctgcttac
     7501 tgcttatctt attagaatga ttgcagcgcc ctatgggctt atgttagtcg cttatggcaa
     7561 acaattgtca atcgcttggc ttccggtgct cgaaggggta ttgaactttg cgttatcgat
     7621 tttctttgtg cgtatatatg gtgccattgg aattgcctac tcgacgttta tttcaggcgt
     7681 attaatcatg gctgtttacg cttgcaagta tcaggctgaa gctgagtata agtcgaaact
     7741 gatatttatc tcgtttctga taatacctat gatgatagcc atttgcctga tcagtttggt
     7801 gatgactcag tcggaaatca tccattgtgt tatttatggc atccaggtgg cttttataat
     7861 agtatttatt atcttcttaa taaaacaagt aaaaagcatc aagaatctgt taaatcaata
     7921 ctaatattgc tatggtgaaa gtgtgaaaaa agttggactc gttactgttt tgttcaattc
     7981 tccaggtgtg cttcctgaat tttattcgag tgttgaaaaa caaaattatc agaacaagca
     8041 tctttatatt gtagataaca gcactaatca ggattctttt aacttaacgc aagaaattct
     8101 cttaggaaca gacataaagt atacctatat taataatgaa ggaaataatg ttggggttgc
     8161 acatgccaat aaccaaggca ttgaacaagc tttaaaagat gggtgcgagt atatattgct
     8221 cactaataat gatctgtttt ttcaacagga agatactatc agcaaaatga ttagtgttgc
     8281 tgagggacga caatgtaaaa ttgtttcacc tgtcatatta agttatccag aaaaaaaagt
     8341 ttggtatgca ggtgcatttt ttaataagaa aaaagctacg gcacctcata tttttgaagg
     8401 agtcaactac aatgatattt gcgctcatat tccaaaagag tgcgattacg caccaacatg
     8461 ttttttactt gtccatgctt cggtgtttac cactataggt atgatggatt ctcgttactt
     8521 tgcttattac gacgatactg attttctcta tcgtgcaaac atagccggca ctcgggtaac
     8581 aattattaat gaacctctag tgtttcataa agttagtact tctaccggag gagggttaag
     8641 tgtattcggc gcatattatt taacgaggaa ccgtatattt ttcgccagaa aaaatattga
     8701 aacaccttat tgtattatta gtattttctt taccattgcg acccgtttgg tttatcctct
     8761 tttccacaaa acaccgtata aagtatacaa ggcttttgtt aaaggaatta tcgacggcct
     8821 aatgttaaaa aataaataac taaggggtta aggtgaacga gatttccagt agcacacttg
     8881 tcaataacta taatttatcg acaactttta ttgtcaagtc acttgttgga ttgttattaa
     8941 ttaattctct ttttagtggt gttgactttt atcagattgg tttgatgaaa ggcctttcca
     9001 gtattgctga ttttggcctg aagattatcc ttgtgttcgt attgctcgtg cttttcaaga
     9061 ttcgcagttt taacttggta aagatattct tttttgcttt tatgcttctc atcggtgctg
     9121 cgcttggagc attctatcat ggtgctgaat atgataaaac ttttgcgctt ttactaaata
     9181 tgatattatg gtttgttata ttcagttatg ctaaagaact tgaacataac tttgatatat
     9241 atcgttttag tgtaaaaata tcaagtttat ttcttggtat cacgttgata ctttacatca
     9301 tggcaaagtt tggttttcat gttaaagtat taggtggagc ttggttatat gatgagaatt
     9361 ttcgctttgc aggtactttc tctgagccaa gtttaaatgg ctattattat ggtttaatgt
     9421 tcttgatggt catgctgtct aatcaaaagt acagactaat tttggcgttt gtgtttgcta
     9481 ttactaccta tatttcaggt gcgaaatttt cattcctaat aataccagtg ctccttgggc
     9541 tggtttattt cttcaaggtg aaaggctttt ataattatac ataccagctc attccgttat
     9601 cttttattgt tctttttgtt agtcttgcgt tcatctacta tgaccttttt gcactgattg
     9661 cagctcattt gtcaaattct gaaacaatga cttatgttac tcgtcttggg ttcccaattg
     9721 tcagcatctg gcatcttagt gattatccat taggaagtgg agtatatggc tttaagagca
     9781 cgctatcacc ttttatacag aactactgtc aggctctttc tggtctggac gtaaattgta
     9841 acgagatgag gtcctatata tcctcggatg atcccagtgc atatgaaagt ttcgcgccta
     9901 aagatgtttt aagtttcatt atattaagtt ttgggcttcc gggcttgctt gtgtttgtcc
     9961 tgagtatatc cttattatca gctagactta aggaaaataa gaattacttt atcattctaa
    10021 tgtatattgt cgcatcaatg ctctttacat taccttttag atttattctt ttttattctt
    10081 tcttcatgtt tcagtgttat atttacagaa tgccgagtaa taaatgatct atattaatgc
    10141 aagattcttg actcagcgta aaaccggtgt gcagcaatat gcattagttt tatgccggga
    10201 actcgttaaa cacattcctg atatctgttt tttgacaccg aaaactgatc tcattgatga
    10261 gaattggcgg gacgagttta atatccttca aataggaaat cgtaatggac atttttggga
    10321 acaaatcgaa ctccctcgtt atctaagaag cgttggtaat ccgttgctgc tctgttttac
    10381 tggcttatca cctgcttttt ataaaaattg ttactttact atccatgata tgtcattgta
    10441 cgcacatccc aagtggttcc gtttgttata tcgtgcagta tacaaggtta actatgctat
    10501 tcaggcaaga ttcgctagag gtatcttcac ggttagtgat ttttcgaaat cagagataga
    10561 gaaatatctg gcggtagact ctgacaagat cactgtatta cgaaattcag tagacacatc
    10621 tattaatatc aagaataata ttgaaaataa agcggttagg cgtaaagaga ttttattggt
    10681 tggtacctta gaacctagaa aaaatataga gtttattatt aattcttttt taaattcgca
    10741 aatcaaaggt tataagctca tcgttgttgg agggttagga gctgctttct caaaggtgac
    10801 tttttctcag catgagaaca ttatattctg tggctatcta tcagacgatg acttagagtt
    10861 aaagtataga caagcttcag tttttgtcta tccttcactg tatgaaggtt tcggccttcc
    10921 tccacttgaa gcgatgcaac gtggctgtcc cgtaatggct tctgaccgcg caagcatccc
    10981 tgaagtttgt ggtgatgctg cttactattt caatccagag gactcatcag attttagtag
    11041 taagttacgt gagctgatcg aaaattatga tgagctcagc cctgtattga tttgcaatgg
    11101 atatcgtaga ctggaacaat ttgacgtacg ggatgttgca tcgaaactta tggatgtcat
    11161 ctctcaaagg aatatttgaa tgaaggtttt acaatttagt aaattttatc ctccagtaca
    11221 cgggggaatt gagcaggttg catttgatat tagcgaagga attacaaaaa gtaatggtca
    11281 gcctgttgac gttctctgtg ttgatcctat tggggaaaga aaagacgacg gaaagtttcg
    11341 ttataaggtt taccgccaga aattgtttgc acagcttttc tcgacaccgc tatctttctc
    11401 cttgatttta cgctggagag cgatcagaaa taactatgat gttattcacg ttcatcttcc
    11461 aaatccaatg gctgtgttgg cgcttttttt atttccacca aaaggaaaaa tagttctcca
    11521 ctggcacagc gatattgtta agcaaaaaaa actattattg ctttttgcgc cgttgcaaaa
    11581 atggatttta gataaatgta cctgtattat agttacaagt cctgtttatg ggcagtcttc
    11641 tccaactttg caaccttatc aggataaaat tgtttgtatt cctattggag ttgatactca
    11701 tgttatgccg ctaaatgatg agcttgaaaa aagtataaaa aaacgctatc aaaataaaaa
    11761 gatagttttt tctcttgggc gtcttgttta ttacaaaggt atggaatatc tgattgatgc
    11821 tgcgaaggag cttcctccgg attacattgt tcttattggt ggaacaggcc cactaattga
    11881 cacattaaag caaaaggttg ctaatgatgg attgtcagat aaagtcgttc ttcttggtag
    11941 catcaattat tcggatttag catcctatta caaatcatgc gacgtttttt gtttgccatc
    12001 tattcatgaa tctgaagcat ttggtgtagt ccagttagag gctatgagtt tctccagacc
    12061 gcttgtgtcg acaaatattc cccgaagtgg cgtggcttgg gttaatcagc ataacgagac
    12121 tggtattgtt gttgagccta atgatgccaa agcacttgct aaaggtatag tttctgttgt
    12181 tcaaaacagt gcaacgttta gtaaaggcgc taaggccaga tttgatacca tgtttactaa
    12241 agaattgatg gttaaaaata taattaattt atattcatct ttaaagtgag tgcaattatg
    12301 ccattatttc ctgttgttat ggccggaggc tctggtagtc gtttatggcc gctttctcgt
    12361 gttctctatc caaaacaatt tctttgtctg gaaggtgaac tgactatgtt acaagcgaca
    12421 atacaccgac tgcagggcat cgtgtgtgaa agtcctgtcg ttatttgtaa tgaacagcac
    12481 cgctttattg ttgctgagca acttcgccag atgaataaac tcaccgagaa tattatcctc
    12541 gaaccgattg gtcgtaatac tgctccggcg atagcgttgg ctgcactagc cgcaactcgt
    12601 gcccagcccg aaggtaaggc gctcatgctg gttcttgccg ccgaccacgt cataaaaaat
    12661 gaagaagcat tccgtgattc cgttcgtaag gcgcttcctt atgctgaaaa tggaaagctt
    12721 gtgacatttg gtatagtccc gaataaagca gagactggct atggttacat tcgccgcggt
    12781 gtgccgtgcg atggtgttga acatgcagat gcattcgacg tcgcgcaatt cgttgagaaa
    12841 cctaatcttg aaacggctga agcctacgtt tcctgcggtg aatactactg gaatagtggg
    12901 atgttcctgt tccgcgccga tcgctatctt gaggaactaa aaaaataccg cccggatatt
    12961 cttgaagcat gcgaaaaggc aatgagtgct gttgagcctg accttaattt tattcgcgtt
    13021 gatgaagatg ctttcagggc ttgtcctgat gagtcagttg actatgctgt gatggagcag
    13081 acttccgatg ctgttgttgt gccaatggat gcgggctgga gtgatgtcgg ttcctggtct
    13141 tcgctatggg atattagcca taagacgcca gagggtaatg tcgtcacagg tgatgtatta
    13201 tcctgcaata ccaaaaacag cttcctgtat tctgaatcag ggcttttgac tacagttggc
    13261 gtgaaagatt tggttgttgt gcagactaag gatgctgtat tgattgccaa tcgtaacgcc
    13321 gttcaggatg tgaagaaaat agtcgaatcg ataaaagcta acgggcgcca cgaacatcat
    13381 actcatcgcg aagtttatcg tccctggggt aaatatgact cgattgatac cgggagccgt
    13441 tatcaggtta agcgattgac ggtaaaacca ggtgaaagta tctcacttca gatgcatcac
    13501 catcgtgctg agcattggat tgtcgtcgca ggagctgcac aagtaacaat taacgaacaa
    13561 actaaactcc tcggtgaaaa cgagtctatc tatattcctg ttgggtcaac acactgtctg
    13621 gagaatcctg gaaagatccc tttagaactg attgagattc gctcaggttc ttatctggga
    13681 gaggatgaca ttgtacgttt tgctgacaaa tatggtcgtg tctgagatta cggtaaaatt
    13741 agtatggaaa aattaacctg ttttaaagcc tacgatattc gcggaaaatt aggcgaagaa
    13801 ctgaatgaag atattgcctg gcgcattggt cgcgcctatg gcgaatttct caaaccgaaa
    13861 accattgtgt taggcggtga cgtccgcctc accagcgaaa ccttaaaact ggcgctggcg
    13921 aaaggtttac aggatgcggg cgtcgatgtg ctggatatcg gtatgtccgg caccgaagag
    13981 atctatttcg ccacgttcca tctcggcgtg gatggcggca ttgaagttac cgccagccat
    14041 aacccgatgg attataacgg catgaaactg gtgcgcgaag gggctcgccc gatcagcggt
    14101 gataccggac tgcgcgatgt ccagcgcctg gctgaagcca acgactttcc tcccgtcgat
    14161 gaaaccaaac gcggtcgcta tcagcaaatc aacctgcgtg acgcttacgt tgatcacctg
    14221 ttcggttata tcaatgtcaa aaacctcacg ccgctcaagc tggtgatcaa ctccgggaac
    14281 ggcgcagcgg gtccggtagt tgacgccatc gaagcccgct ttaaagccct cggcgcaccg
    14341 gtggaattga tcaaagtgca caacacgccg gacggtaatt tccccaacgg tattcctaac
    14401 ccgctactgc cagaatgccg cgacgacacc cgcaatgcgg tcatcaaaca cggcgcggat
    14461 atgggcattg cctttgatgg cgattttgac cgctgcttcc tgtttgacga aaaagggcag
    14521 tttatcgaag gctactacat tgtcggcctg ctggcagaag cattcctcga aaaaaatccc
    14581 ggcgcgaaga tcatccacga tccgcgtctc tcctggaaca ccgttgatgt ggtgacggcc
    14641 acaggtggca ccccggtaat gtcgaaaacc ggacacgcct ttattaaaga acggatgcgc
    14701 aaggaagacg ccatctacgg tggcgaaatg agcgcccacc attactttcg tgattttgct
    14761 tactgcgaca gcggcatgat cccatggctg ctggtcgccg aactggtgtg tctgaaagga
    14821 aaaacgctgg gagagctggt gcgtgaccgg atggcggcgt ttccggcaag cggtgagatc
    14881 aacagcaaac tggcgcaacc cgttgaggcg attaaccgcg tggaacagca ttttagccgt
    14941 gaggcgctgg cggtggatcg caccgatggc atcagcatga cctttgccga ctggcgcttt
    15001 aacctgcgct cctccaacac cgaaccggtg gtgcggttga atgttgaatc gcgcggtgat
    15061 gtgccgctga tggaagaaaa gacaaaactt atccttgcgt tattgaacaa gtaattcagt
    15121 aatttcatat aaataagttt taaaagacgg aaaagatgag atattcagtg tggtatagca
    15181 aaggtaatgc tattcaccat ctctatgagt gagttaacat ctataccaca tttaagccgc
    15241 acactcggcg gtgaccaccc ctgacaggag taaacaatgt caaagcaaca gatcggcgtc
    15301 gtcggtatgg cagtgatg
//