<pre>LOCUS       KJ778809               14124 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain H 19 serotype O84:H21 O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ778809
VERSION     KJ778809.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14124)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14124)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Swimley,M.,
            Baranzoni,G.M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="H 19"
                     /serotype="O84:H21"
                     /db_xref="taxon:562"
     misc_feature    1..14124
                     /note="O-antigen gene cluster"
     gene            complement(239..1663)
                     /gene="manM"
     CDS             complement(239..1663)
                     /gene="manM"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="AJE24481.1"
                     /translation="MLNVKKIINDSNIAFGTSGARGLVIDFSHDVCAAFTHAFLSVID
                     DKYNFNKVALAIDNRPSSYEIAQACAYAIKQHGFEVEYHGVIPTPALAHYSMQKNIPC
                     IMVTGSHIPFDRNGLKFYRPDGEITKEDELAIINSEYTFSPVGVLPHLETSTQGADYY
                     LERYVSLFNPEILKGKRIGVYEHSSAGRDLYAPLFNQMGAEVISLGRSDEFVPIDTEA
                     VSDEDRILAREWSKKYNLDAIFSTDGDGDRPLVADENGEWLRGDILGLLTAIELNIKA
                     LAIPVSCNTAIEESNKFASIQRTKIGSPYVIAAFADLAKQFDSVAGFEANGGFLLASD
                     LQINGKELKSLPTRDAVLPALMLLIASRNSTISQLINNLPQRFTWSDRVKNFPSDSSQ
                     QIIKNAISSPNNFFNSLGYESLSCSAIDETDGARFTLNNGDIIHLRPSGNAPELRCYA
                     EASDENQAKQYVTNVLGNITSLIS"
     CDS             complement(1669..2415)
                     /codon_start=1
                     /transl_table=11
                     /product="PGL/p-HBAD biosynthesis
                     glycosyltransferase/MT3031"
                     /protein_id="AJE24482.1"
                     /translation="MRISIITATYNSEKTLLDTLLSLEKQTHPDIEYIVVDGASKDNT
                     IKLIKRNCTKVSKIICEPDKGIYDALNKGIQVASGDVIGFLHSDDLLAYDDAIADIAK
                     TFESTGCDAIYGDLEYVAQNDTTKRIRLWKSGSFSRFKMKVGWMPPHPSFYMKRECYS
                     QFGSFSLDYRISADYDSLLRYILKQRISIAYLPKVLVKMRVGGISNRSVSSMVKKSME
                     DIRIMKQNGIIWPLALAYKNISKLPQFIKK"
     gene            complement(2415..3821)
                     /gene="manC"
     CDS             complement(2415..3821)
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase 1"
                     /protein_id="AJE24483.1"
                     /translation="MLLPVVMAGGSGTRLWPLSRTLYPKQFLSLNSRLTMLQETLRRL
                     DKVEHKPALVICNESHRFIVAEQLRKESLKHSGILLEPVGRNTAPAVALAALQAMVTG
                     DDPILLVLAADHEIQDEDNFIAAILAAKNFAEQGKLVTFGIIPTSPETGYGYIKSGES
                     LDGQGYKVAAFVEKPDLHVAQRYISDGGYLWNSGMFMFRASVFIDELKKFRPDILASC
                     QRSLSSSIQDLDFIRLDSASFSCCPEESIDYAVMEKTAEAVVVPLNAQWSDVGSWSAL
                     WEISSKDQSGNAIRGDVLVEDATDSYLYSQHRLIGAVGVKDLIVVETKDAVLVAHKDK
                     VQQVKNIVAQLKKNNRTEYLQHREIFRPWGSHDTIAEGPRFQVKHVIVLPGHITAKQI
                     HYHRTEHWIVVSGTAKVHLEDKTYLVSENESTYIPVGVPHAIENPGKIPLEIIEVRSG
                     VYLEEDDVIRVSSSGVGY"
     gene            complement(3827..4288)
                     /gene="gmm"
     CDS             complement(3827..4288)
                     /gene="gmm"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="AJE24484.1"
                     /translation="MNKRLERELFKTIVEHTPLISIDLIIRNDKGEALLGQRLNRPAQ
                     NYWFVPGGRIYKDESFEIAFKRITLEELGVQISLNDALFLGVYEHFYNDNFFEAEFST
                     HYIVHGYEIQLKPQQLHLPTVQHNSYKWFDVVTLLNSTIVHQYTKNYFIPR"
     gene            complement(4291..5256)
                     /gene="fcl"
     CDS             complement(4291..5256)
                     /gene="fcl"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="AJE24485.1"
                     /translation="MTKKRIYVAGHRGMVGSAICRQLSQRDDIELVVKTHKELDLTVQ
                     KDVDAFFEQEKIDQVYLAAAKVGGIYANNTFPAEFIYQNLMIESNIIHSAHKAGIQKL
                     LFLGSSCIYPKFAEQPMNESALLMGILEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGINDNFHPENSHVIPALMRRFHEAKESGAPEVIVWGTGTPMREFLYVDDMA
                     AASVHVMELDEAIYQQNTQPMLSHINVGTGVDCSIREMAETMASVVGYQGKIVFDFTK
                     PDGTPRKLMDVTRLKNLGWQYRYNLHEGLSLTYKWFIGNINSFRG"
     gene            complement(5260..6384)
                     /gene="gmd"
     CDS             complement(5260..6384)
                     /gene="gmd"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="AJE24486.1"
                     /translation="MKNKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTER
                     VDHIYQDRHNENPDFILHYGDLTDSSNLIRLIKEIQPDEVYNLGAQSHVAVSFESPEY
                     TADVDAMGTLRLLEAIRICGLEKKTRFYQASTSELFGLVQEIPQRETTPFYPRSPYAV
                     AKMYAYWITINYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANVSQGIEKCLY
                     LGNMDSLRDWGHAKDYVRMQWMMLQQERPEDFVIATGKQISVREFVRMAAKEVGLELQ
                     FSGKGIDEIATVVNKTSDCAIGVNVGDVIVRVDPRYFRPAEVETLLGDPSKAKKLLGW
                     EPEITVEEMCAEMVASDLAKAKQHALLKSHGYDVAVSLER"
     gene            complement(6374..7645)
                     /gene="mfpsA"
     CDS             complement(6374..7645)
                     /gene="mfpsA"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylfructose-phosphate synthase"
                     /protein_id="AJE24487.1"
                     /translation="MKVKIISYSLSKGGAAKAAKNFLHLASKNKKYEAEMISVSGIIK
                     NGVLFKSSKLSVFWHYSKMLVSRVLTYFILNDKSIKYSLNIFDSNYVLKHIRVEDGQR
                     EVIHINWVNNDTISLFSLSKLFENKRKRIILTLHDEWFYCASEHYAKYNSLCYVTGYD
                     DSNFINSYIFELKKRINFENVVITVPSYWLYERAKKSYLLSNCDIYILPNYIDTNIFT
                     ELKDIRSKRYRILDVPEDAFIIGFGAVSGGANPLKGFDLLVSALDEVLSKINDKARIV
                     MVTFGANDIDKRVTSLGCHIINMGVISNSHNMAEIYNYLDVMIVPSRVESFGQVAAES
                     LACKTPVIAFDYSGIKDIVTHQRSGLLAEPFSVDSLALNINSFMNMTEKERYVYGENG
                     RDDVVRKFGETVVSEIYFKLIDYVRGDEYEK"
     gene            complement(7642..8763)
                     /gene="tagF"
     CDS             complement(7642..8763)
                     /gene="tagF"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol:poly(glycerophosphate)
                     glycerophosphotransferase"
                     /protein_id="AJE24488.1"
                     /translation="MSKKIKYIINFAMSLFILPFFYIEKKIIGKKIWLCGAGNGYYEN
                     NIASIHDYILKSLPLAKNKIFFVTTNRELLSGDVAFPVLIRGHLKTYALSILADYLIF
                     DTCNSDIAPGIHRYLSALKVNVNHGFEGLKKLPEDYYAKIDADIHCASSMREKYIKVF
                     ECGAANEKVFITGYPRFDRIVSRENSHVKKILFFPTWRSWLESLSNDELNNSTYVRSI
                     YDFLYSERLKEYLSENNIIMYYKPHHKISHLKLDLSSCKNIILLKDYDDLTHYICESD
                     LLITDYSSVAWDFLYSNREVIFYIFDIDEYILKQGLYYDVRTSLRNYGGTPDEIINLL
                     KKERKEVDLNLSSMAGEFFEYRDNKNSERILKLICEYKI"
     gene            complement(8765..9916)
                     /gene="wzy"
     CDS             complement(8765..9916)
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AJE24489.1"
                     /translation="MAIYIFISISCLISYVLSNNDKNSLSPRFLLIILTLFTGLSYTN
                     GWDWYGYLDFYNNIQNEGVSAIYEYNIYGIEYLYLIYLYIVGLSGGSFSLFILLNAII
                     INFLIYSFCKRAYVNYSLFMFIFIAASYLRLELSTIRQGLAVVIVIYSYSLLLNSKIN
                     LSLLFIFLAICFHRSAAIVLLFFPFVYYNSARNIHYFIVILTIPFVILSGELNVVFIK
                     ILNAFNSSLLNAYISKLIIYLSLNKQAIINPQAMALLVFYFICIRVCDVKNKKQIIFL
                     NIIACQVIISFYFIFLTQLIIMRLVYYFQVGWICWVIILYKEYFRPKWLCFFLICLLF
                     ITKSILNFRYEQDRAVFFPYYNVISSYLDDNYGRSRSFILNKADELFKE"
     CDS             complement(9918..11057)
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferases group 1"
                     /protein_id="AJE24490.1"
                     /translation="MLKVGIPVLFSEQHWMGGINYFRSLISAFSLVEQNDIELIIFCE
                     KDGLFNTTKLNNVRQVIIPELLPRKLSKRIINKVYGVNFELYKAIKNESVDILSHTQI
                     NKRLACKTMWWKPDFQEKYYPEFFNEKDLFLRNQSVVNNALRGYLLFSSNDAKNDFFK
                     FYNTLPTQNINVLQFVPEIEISSSQDENDRIIAVKEKYGISGDYFFLPNQFWKHKNHE
                     LVIKAIITGDIPAKVVATGASNDYRGGRHIELINQLLKQDSAKKFKLLGLVDRDDVNL
                     LMKGAIAVINPSRFEGWSTTVEEAKYLGKRLILSDIPVHHEQNPPDSLYVSCDDVSGM
                     ISAMRKIIDEYDTEKEIFRAKKAAEQYKFKRKEFGLSYYNILKSL"
     gene            complement(11051..11953)
                     /gene="rmd"
     CDS             complement(11051..11953)
                     /gene="rmd"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-6-deoxy-D-mannose reductase"
                     /protein_id="AJE24491.1"
                     /translation="MILGAGGFIGSHLCNSCIQNGATVYALGRNLDNVKIKNQRFIKL
                     SSNEITEKTLFDVAQPDYVYFLMGAASVAKSIEAPLYDFNQSLPPLLVILEKLRKEWI
                     GTRLIFISSAAVYGENASERTSVSSLPMPLSPYGLNKKISETYVQYYMKHYDISAKII
                     RPFSVYGPGLKKQLIWDVLNKIKDKQHHYFGSGNEMRDWIYIDDFIAVLFNYVSNYAL
                     MSDLINVGTGIPIKVKNVIEQLYTLTDTFEIPSFSDGGKEGDPKHLVSNYEEQDFLRE
                     LLKTPLELGLRNTVDWFKNQEEQC"
     CDS             complement(11973..12584)
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="AJE24492.1"
                     /translation="MKKFISRLLYNPYKKIKKDSRIEFNSGILDKNFRVHFYCSESRA
                     KAYIGNNCILKNEIIFERECGVVTIGDNTFINKNTKIISVNRISIGSNVTISYGVIIY
                     DHDSHSLDYRERQKDILKILETRDPNNDIENKNWDCVKSLPVIIGDNVWIGFQAAILK
                     GVTIGEGAVVAARAVVTKDVPAWSVVAGNPAKVVKEIPRDMRK"
     gene            complement(12584..13978)
                     /gene="wzx"
     CDS             complement(12584..13978)
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AJE24493.1"
                     /translation="MLSKIKNVIDRIVIIFKPIAADLTQKEKDEVIRLRGVYLTAIIS
                     LLSKIISMISIFITIPAILNYLGEELFGIWMTLSSIIAMLTFADMGIGNGVVNKLSKA
                     IYSNSVNDIRKIITNAVTVLLIIATSINLLFYAATPLVSWNRILNLSEKIDSHSIEHA
                     LFVFVFCFSLNVLFSIVQKIQLAYQLGFIASIWQILGSVFSLGLLFLFINLKLEMAWL
                     VFSIAGIPAIFQLLNYLHFSLVICKTGFFKISFIKIAEMKSLLSTGVLFFALQISMVC
                     TYTSDNIILNTIIGAEAVTEYSVHVRLFSVIPILMGMVLIPLWPAYSSARIKKDIKWI
                     RDVWNKSIMMALLVSVFIGGSIILFLPDIFDLWLKNKIQPTYTLAYMLFVWKLFESVG
                     LAISCFLNGMDLIKQQAFIAVVTAVVAISLKILFVYQIGITGVLLGTLIPFVLLTFIP
                     TLFISLGFLRKGMC"
ORIGIN      
        1 catcactgcc ataccgacga cgccgatctg ttgctttgac attgtttact cctgtcaggg
       61 ggtgttcacc gcgatgtatg cgcgacttga atatgattga gatgttatct caagttgcac
      121 gaatgtgtgt agtgctgaat ttcattaatg caacaaatac cgttcttcaa ttctgtatac
      181 cggcatctac gtgtaatcaa acctacgcac acatataagc gtagataaac ctataacatc
      241 aagaaatcaa agaggtaata tttcccagca cattcgtaac atattgctta gcctgatttt
      301 catcactggc ctcagcataa caacggagtt ctggagcatt accggaagga cggagatgta
      361 taatatcacc attatttaaa gtaaatcttg caccatccgt ttcatcaata gcggaacagg
      421 ataatgattc ataacctaaa ctattaaaga aattattggg tgacgatatg gcatttttta
      481 taatttgttg acttgaatct gaagggaagt ttttaactct atctgaccaa gtgaatcgct
      541 gaggaagatt attaatcagt tgagaaatag tactattgcg agaagctatt aagagcatta
      601 atgctggtaa cacagcatct cgtgtaggta acgattttaa ttccttgcca ttaatttgta
      661 aatcagaggc aaggagaaaa ccaccattag cttcaaaacc agcgactgaa tcaaattgct
      721 tagcaagatc tgcaaatgct gcaattacat aaggagaacc tattttcgtt cgttgtatac
      781 ttgcaaattt attagactct tcaattgctg tattacaact cactggaata gccaacgcct
      841 tgatattaag ttcaatagca gtaagtagtc ccagaatatc gcctcttaac cattcaccat
      901 tttcatcggc aactaaagga cgatcaccat cgccatctgt tgagaaaata gcatcaagat
      961 tatatttttt agaccactct cttgcaagta tacgatcttc atcgcttact gcttccgtat
     1021 caatcggaac gaactcatca ctcctaccga gggaaatgac ctctgcaccc atttgattaa
     1081 aaagaggagc atataaatcg cgtcccgcac tagaatgttc atatacccct attcttttcc
     1141 cctttaaaat ctcgggatta aaaagagaaa cataacgctc gaggtagtag tctgcacctt
     1201 gagtgcttgt ttcaagatga ggtaatacac ctacaggcga aaatgtatac tcactattta
     1261 taattgcgag ctcatcctct ttagtgattt caccatcagg tctgtagaat tttaagccat
     1321 tacgatcgaa agggatatga ctccctgtga ccattatgca gggaatgttt ttctgcatcg
     1381 aataatgagc taatgcagga gtcggaatta caccatgata ttcaacctcg aatccatgtt
     1441 gttttatagc atacgcgcat gcctgagcaa tttcgtaact acttggacgg ttatcaatgg
     1501 ctaaggcaac tttattaaaa ttgtatttgt catcaataac agaaagaaac gcatgagtga
     1561 acgcagcaca aacatcatgg gaaaaatcga tcactaaacc gcgagcacca ctagtaccga
     1621 acgcaatatt actatcgtta atgatttttt taacatttaa catgatgatt actttttaat
     1681 aaattgagga agtttggata tatttttata cgctaaagca agtggccaga taataccatt
     1741 ctgtttcata atacgaatgt cttccattga cttcttgacc attgaagata ctgaacgatt
     1801 gctaattcca ccaacacgca tcttcactaa gacttttggt aaatatgcta tcgaaatacg
     1861 ttgttttaaa atatatcgta ataatgagtc ataatcagca gatattcgat aatctaaaga
     1921 aaaactacca aactgactat aacattcgcg tttcatataa aatgatgggt gtggtggcat
     1981 ccaacccact ttcatcttga aacgactaaa tgaaccactt ttccataatc taatacgttt
     2041 agtcgtatca ttttgggcaa catactccaa atcgccataa atagcatcac accctgtact
     2101 ttcaaatgtt tttgctatat ctgcaatagc atcatcataa gctagtaaat catcagaatg
     2161 taaaaagcca ataacatcac ccgaagctac ttgaatacct ttattcagag catcataaat
     2221 gcccttatcg ggttcgcaaa tgatttttga aacttttgta caattcctct tgatcagctt
     2281 aattgtatta tctttcgatg ctccatctac aactatatat tcgatatctg gatgcgtttg
     2341 tttttctaga gaaagtaatg tatcaaggag tgttttttca ctattataag tggctgtgat
     2401 aatagaaatt cgcattagta tcctactcca gaagaagaaa ctcttataac atcatcctct
     2461 tccaggtaga ctcctgatct aacctcaatt atttcaagcg ggatcttgcc aggattttca
     2521 atggcatgtg gaacaccaac aggtatatat gttgattcat tttcagaaac aaggtaagtc
     2581 ttatcttcaa gatgaacttt agctgtcccc gagacaacaa tccaatgttc agtacgatgg
     2641 taatgaatct gcttagcggt aatgtgacca ggcaatacaa tcacatgttt cacttgaaag
     2701 cgcggccctt cagctatagt atcatgactg ccccaaggcc gaaatatttc ccgatgctgt
     2761 aaatattctg tgcgattatt ctttttaagt tgagcgacga tgtttttaac ttgctgaact
     2821 ttatctttat gagcaactaa tacggcatct ttcgtttcaa caacaatcaa atcctttacg
     2881 cccacggcac caataagtct atgctgcgaa taaagataac tatctgtagc atcttcaacc
     2941 aatacatcac cacgaatggc attaccgctt tgatcttttg aacttatttc ccacaatgca
     3001 gaccatgacc caacatcact ccattgtgca tttaatggaa cgacgacagc ttcggctgtt
     3061 ttttccataa ctgcatagtc aatagactct tcaggacagc aagaaaatga agcactatcc
     3121 agacggataa aatctaaatc ttgtatcgaa gaggacaagg agcgttggca actggctaaa
     3181 atatctggtc ggaatttttt cagttcatcg ataaatacag atgctctgaa cataaacatc
     3241 ccgctattcc aaagataacc gccatctgat atgtaccgct gagctacgtg aagatctggt
     3301 ttttcaacaa aagccgcaac tttgtaacct tgtccatcca gagattcacc tgacttaatg
     3361 taaccatagc cagtctctgg ggatgttgga ataataccaa acgtaacaag cttaccctgc
     3421 tctgcaaaat tctttgcagc gagaattgca gcaatgaaat tatcctcatc ctggatttca
     3481 tgatcagcag caagaaccaa aagaatagga tcgtctccag ttaccatagc ctgaagtgct
     3541 gcaagtgcta cagcaggcgc agtattacga ccaacaggct caagcaaaat accgctatgc
     3601 tttaaactct ctttacgcaa ttgttcagca acgataaagc gatgtgattc gttacaaatg
     3661 accaaagcag gtttatgttc gaccttatca agccgccgca atgtctcttg caacatggtt
     3721 aaacgactat ttaaagacag aaattgtttc ggataaagtg tacgtgaaag aggccataat
     3781 ctggtaccag aaccaccggc catgacaacg ggaagtagca tatctattac cttggtataa
     3841 aatagttttt ggtatattga tgaactatag tgctattaag caacgttact acatcaaacc
     3901 acttgtagga attatgctgg accgttggta ggtgaagttg ctgaggttta agttggattt
     3961 catatccatg cactatatag tgtgtagaaa attctgcttc aaaaaaatta tcattgtaaa
     4021 aatgttcata caccccaaga aataaggcat cattaagact aatttgaacg cccaactctt
     4081 caagtgttat ccgcttaaat gcaatctcga atgactcatc cttataaatt cgccctcctg
     4141 gcacaaacca ataattttgt gctgggcgat tcaggcgctg ccccagcagc gcctctcctt
     4201 tatcgtttct aattatgaga tcaatcgaga ttagaggagt atgctcaact attgttttaa
     4261 atagctcacg ttccaatctc ttgttcataa ctatccccga aaagaattaa tattcccaat
     4321 aaaccattta taagttaatg ataagccttc atgtaaatta tagcgatatt gccagcccaa
     4381 gtttttgagc cgggtaacgt ccataagttt acgcggagtg ccatcaggtt tagtaaaatc
     4441 aaaaacaatt ttaccttgat aacccaccac agaggccatt gtttcagcca tttcacgtat
     4501 agaacaatct acacccgtac caacattaat atgggataac ataggctgtg tattttgttg
     4561 ataaattgct tcgtcaagct ccattacatg gacggatgcg gcagccatat catcaacata
     4621 taaaaactca cgcatcggtg ttccggttcc ccagacaata acctctggcg caccactctc
     4681 ttttgcttca tggaatctac gcatgagtgc cggaataaca tgagagtttt caggatggaa
     4741 attatcattt atgccataaa gattagtagg cattacactg cgataatcac ggccatattg
     4801 gcggttataa gattcacata attttatgcc cgcaatttta gcaattgcgt atggttcatt
     4861 agttggctca agtatgccca ttaaaagcgc tgactcgttc atcggctgct ctgcgaattt
     4921 aggataaata cagcttgagc ctaaaaaaag taatttttga attccggcct tgtgagctga
     4981 atgaataata ttactctcaa tcatgagatt ctgatagatg aattctgccg gaaatgtatt
     5041 gttggcataa atgccaccaa ctttagccgc agcaagataa acctgatcaa ttttctcttg
     5101 ctcaaaaaat gcatcaacat ccttctgtac ggttagatcg agttctttgt gtgttttgac
     5161 cactaattcg atatcatcac gctgtgataa ttgacggcaa atagcagagc caaccatacc
     5221 tcggtgacca gcaacgtaga tacgtttttt tgtcatacct taccgctcca gagaaactgc
     5281 aacatcgtaa ccatggcttt tcagtagtgc atgctgtttt gctttcgcca gatcactggc
     5341 aaccatttcc gcacacattt cttcaacagt aatttctggt tcccaaccca ataatttttt
     5401 agctttactt ggatcaccaa gaagtgtttc aacttccgca gggcggaaat agcgtggatc
     5461 aacacgaaca attacatccc caacatttac gccaatagca cagtcagatg ttttgtttac
     5521 aacagtagct atttcatcaa tacctttacc agagaactga agttccaagc caacttcttt
     5581 agccgccatt cggacaaatt cacgtacaga aatttgttta ccggtagcga ttacaaaatc
     5641 ttccgggcgt tcttgttgaa gcatcatcca ctgcatacgg acataatctt tagcatgtcc
     5701 ccagtcacgc aatgagtcca tgttaccaag atagagacat ttttcgattc cttgcgaaac
     5761 attagcaatt gcacgagtaa ttttacgtgt aacaaatgtt tcgccacgac gtggagattc
     5821 gtggttgaac aagataccgt tacaggcata cataccataa gattcacgat agtttatagt
     5881 aatccagtat gcatacatct tagcaacagc ataaggcgaa cgaggataga acggtgtagt
     5941 ttctcgctgc ggaatttctt ggactaagcc gaagagttca gaagttgatg cctgatagaa
     6001 acgcgtcttt ttctctagtc cacaaatacg gatcgcttcc agcagacgta aagtccccat
     6061 agcatctaca tcagcagtat attcaggtga ttcaaatgaa actgctacat gagattgtgc
     6121 tccaaggtta taaacttcat ctggttggat ttctttaatc aagcgtatca ggtttgatga
     6181 gtccgttaag tcaccataat gaagtatgaa atctgggttt tcattatgac gatcctgata
     6241 gatatgatct acacgttcag tattaaaaga agatgctcgg cgttttatac cgtgcacttc
     6301 ataacccttt tcaagaagaa attctgccaa gtaagaacca tcttgaccgg taacaccagt
     6361 aattagagct actttatttt tcatattcat ctcctctaac ataatctatc aatttaaaat
     6421 aaatttctga aaccaccgtt tctccaaatt ttcgaactac atcatcgcga ccattttcac
     6481 catatacata tctctctttc tccgtcatat tcataaatga atttatattt agtgcgagtg
     6541 aatcaacaga aaatggttca gctaataaac cactccgttg atgagtaaca atatctttaa
     6601 ttcctgaata atcaaatgca ataaccgggg ttttacaagc taaactttca gcagctactt
     6661 gcccaaagga ttcaacgcgt gaaggaacaa tcatgacatc caaataatta taaatctcag
     6721 ccatattatg gctgttagag ataactccca tatttataat gtggcatccc agtgatgtta
     6781 cacgtttatc tatatcgtta gcaccgaacg tcaccattac aattctagct ttgtcattta
     6841 ttttacttaa tacttcatcc aaggcagaaa ccaagagatc aaaccccttc aaggggttgg
     6901 caccgccact aacagcacca aatccaataa taaaagcatc ctcaggaaca tcaagtattc
     6961 tataacgttt tgaacgaatg tcctttaact ctgtaaaaat attagtatcg atataattag
     7021 gcaatatata tatatcacaa ttacttaaca aataactttt ctttgctcgc tcatacaacc
     7081 aatatgatgg aacggtaatt acgacattct caaaatttat tctctttttt aattcaaaaa
     7141 tatagctatt aataaaattg ctatcatcat atcctgttac ataacataat gaattatatt
     7201 tagcatagtg ctctgacgca caataaaacc attcatcatg taacgttaat attattcttt
     7261 ttcttttatt ttcaaataat ttacttaatg aaaataaaga tatggtatca ttattcaccc
     7321 aattaatatg gataacttcc ctttgcccat cttccactct gatatgtttt aaaacataat
     7381 tagagtcaaa aatattcaat gaatatttta tacttttatc atttaaaata aaataagtga
     7441 gtacccggct tactaacatt tttgagtaat gccaaaacac tgataatttt gatgatttaa
     7501 ataaaacacc attttttata atacctgata cacttatcat ctctgcttca tattttttgt
     7561 ttttcgaagc aagatgtaaa aaattttttg ctgcttttgc cgctccgcct ttggataaag
     7621 aataagaaat tatttttact ttcatatctt atattcacag attaatttta gaattcgctc
     7681 agaattttta ttatctcgat actcaaaaaa ttcccctgcc atacttgata aatttaaatc
     7741 aacttcctta cgttcttttt taaggagatt aataatttca tcaggtgtgc cgccataatt
     7801 tcttaaggaa gttctgacat catagtataa accttgtttt aatatatatt catcaatatc
     7861 aaatatatag aaaattactt ctctattaga gtaaaggaaa tcccacgcaa cgcttgaata
     7921 atctgtaata agcaaatctg attcacatat ataatgtgta agatcatcat aatcttttag
     7981 caaaataata tttttacaag aagataaatc taatttcaaa tggctaattt tatgatgtgg
     8041 cttataatac attataatgt tattttcact caaatattct ttgagtctct cactgtatag
     8101 aaaatcataa atagatctga catacgtgga attatttaac tcatcattgc ttagactctc
     8161 caaccatgat ctccatgttg gaaagaaaag aatttttttc acatgactgt tttcacgtga
     8221 aacgattcga tcaaatctag gataacctgt aataaatact ttctcattcg ctgctccaca
     8281 ttcaaatacc ttaatatatt tctctctcat tgaagaagca caatggatgt cagcatcaat
     8341 tttcgcataa tagtcttcag gaagcttctt taagccttca aagccatgat taacatttac
     8401 cttcaaagca gaaagatacc ggtgaattcc aggagcaata tctgagttac aagtatcaaa
     8461 gattaaataa tcagctaaaa tactcaacgc ataagttttt aaatgccctc taataagtac
     8521 aggaaaggct acatcaccgc ttaaaagttc ccgatttgtt gttacaaaaa aaattttatt
     8581 tttagctaaa ggtaaactct tcaaaatata atcatgaatg ctagctatgt tattttcata
     8641 atagccattt cctgcgccac ataaccaaat tttttttccg attatctttt tttctatata
     8701 gaaaaatggt aatataaaaa gtgacattgc aaaatttata atatacttaa ttttctttga
     8761 cattttactc cttaaaaagc tcatctgctt tattcaatat aaaagatcta cttctcccat
     8821 aattatcatc caggtatgaa gatattacat tataatatgg aaaaaaaacc gctctatctt
     8881 gctcatatct aaaatttaat attgacttgg taataaacag aagacatata aggaaaaaac
     8941 ataaccattt aggtcggaaa tactccttgt ataatattat tacccaacat atccatccaa
     9001 cttgaaaata ataaaccaga cgcataataa tcaattgtgt taaaaatata aaataaaaag
     9061 aaattataac ctgacaagca attatattta aaaatataat ttgttttttg tttttaacat
     9121 cacaaaccct gatacaaata aaataaaaga ctaataatgc catagcttgt gggttaataa
     9181 ttgcttgttt gtttaatgac aaataaatta taagcttcga tatatatgca tttaataatg
     9241 atgaattaaa agcattcaat atttttataa atacaacatt taattccccc gaaagaataa
     9301 caaatggtat agtaagtatg acaataaagt aatgaatatt tcttgcacta ttataataaa
     9361 caaacggaaa gaataataat actatagcag cgcttctatg aaagcaaata gccagaaata
     9421 taaacaataa ggataaattt atttttgagt ttaataaaag cgaatatgaa tatataacta
     9481 taacaacggc caatccttga cgtatagtac ttaattccaa cctcaaatat gatgcagcta
     9541 taaaaataaa catgaacaag ctataattaa cataagctcg tttgcaaaaa ctataaatga
     9601 gaaaatttat tattattgca ttcaataata tgaataagct aaaacttcct ccacttaggc
     9661 caacaatata caaatatatt aaatataaat actcaatgcc atatatatta tactcatata
     9721 ttgcacttac cccttcattt tgaatgttat tatagaagtc aagataaccg taccaatccc
     9781 atccatttgt ataagataac cccgtaaata aagttagtat tattaacagg aatcttggac
     9841 tcaatgaatt cttatcatta ttcgacaaaa catatgatat taaacatgaa atgcttataa
     9901 atatatagat ggccatatta tagtgacttt aagatattat agtatgagag accaaattcc
     9961 tttcttttaa acttatattg ctcagcagcc ttctttgcac gaaatatttc cttctctgta
    10021 tcatattcat ctattatctt gcgcatagca gaaatcattc ctgatacatc atcacatgat
    10081 acatataatg aatcaggagg attttgttca tggtgtactg gtatatcaga taatattaat
    10141 cttttaccta aatattttgc ctcttcgacc gtcgtactcc acccctcaaa ccgagatggg
    10201 ttgattacgg ctatagcacc tttcatcaaa agattaacat catccctatc aactaaccct
    10261 aataacttga attttttagc agaatcttgt ttaagtaatt gattgattaa ttctatatgc
    10321 ctccctccac gataatcatt agatgcccca gttgccacga ctttagcagg tatatctcct
    10381 gtaataatgg cctttatcac taattcatgg tttttatgtt tccagaattg gtttggtaaa
    10441 aaaaaataat ctccactaat tccatatttt tcttttactg ctattattct atcattttca
    10501 tcttgtgaac tgcttatttc aatttctgga acgaattgaa gcacattaat attttgtgtt
    10561 ggcaaagtat tatagaactt aaaaaaatcg ttttttgcat cattactact aaacagcaga
    10621 tatccacgta aagcattatt aactacagac tgatttcgta gaaataaatc tttctcatta
    10681 aaaaattcag gatagtattt ctcttgaaaa tcgggcttcc accacatggt tttgcaagct
    10741 aaccgtttat ttatctgggt atgagataaa atgtcaacac tttcattttt aatagctttg
    10801 tataattcaa aattcactcc ataaacttta tttattatac gtttagacaa ttttctagga
    10861 agaagctcag gaataattac ctgtcgaaca ttattaagtt tggttgtatt gaacaatcca
    10921 tctttttcac aaaaaattat aagttcaata tcattttgtt caacaagtga aaaagcagaa
    10981 attagacttc taaagtagtt gatccctccc atccaatgct gctcactgaa caatacagga
    11041 ataccgactt ttaacattgt tcctcctggt ttttaaacca atcaactgta tttctcaatc
    11101 ccaattcaag aggcgttttt aaaagttctc ttaaaaaatc ttgctcctca taattactta
    11161 ccaagtgctt tggatcgcct tccttaccac catcactaaa cgagggaatt tcaaatgtat
    11221 cggtcaatgt gtacaattgt tcaataacat ttttaacctt tattggaata cctgtaccta
    11281 catttatcag gtcactcatc aaagcataat tactaacata attaaacaaa acagcaataa
    11341 aatcatcaat atatatccaa tcacgcattt cattaccgct accaaaataa tgatgctgct
    11401 tatcttttat tttattcaat acatcccaaa taagttgctt ctttaaacca ggcccataga
    11461 cagaaaaagg tcttattatt ttcgcactaa tatcatagtg tttcatataa tattgcacat
    11521 acgtttctga gattttctta tttaatccat aaggggataa aggcataggt aaactactta
    11581 cacttgttct ttcactcgca ttttcaccat atactgcagc acttgagata aaaatgaggc
    11641 gcgtccctat ccattctttt cgtaattttt ccaaaatcac caatagtggg ggtaaactct
    11701 ggttaaagtc atataatgga gcttctatag attttgctac agatgccgcg cccattaaaa
    11761 aatacacata atctggttgt gcaacatcaa ataatgtctt ttcagtgatt tcatttgaac
    11821 ttaattttat aaacctctgg tttttgatct tcacattatc taagttacgc ccaagtgcat
    11881 aaactgtagc tccattttgt atacatgaat tacacagatg agaaccaatg aatccacctg
    11941 cccccaaaat cattatagtt atatttttca acttacttcc tcatatctct tggtatttct
    12001 ttcacaacct tcgctggatt gcctgccacg acagaccacg ccggaacatc ttttgtaact
    12061 accgctcttg cagcaacaac cgcgccctca cctatagtaa caccttttag tatcgcagct
    12121 tgaaaaccta tccaaacgtt atcacctata attactggta aacttttaac acaatcccaa
    12181 tttttatttt ctatatcatt atttggatct cgagtctcta atattttcag aatatccttt
    12241 tgcctttccc gataatctag cgagtgtgaa tcatggtcat atattattac accataagat
    12301 atggtaacat tacttcctat actaattcta ttaacactta taatcttcgt gtttttgtta
    12361 atgaatgtat tatcaccaat agtgacgaca ccacactctc tctcaaatat aatctcattt
    12421 tttaggatgc aattatttcc gatatatgcc ttagcacgac tttcgctaca gtaaaaatgc
    12481 acacggaaat ttttatccaa aatcccagaa ttaaattcaa ttcgagagtc tttttttatc
    12541 tttttgtaag gattatagag caatcttgaa ataaactttt tcattagcac attccttttc
    12601 tcaaaaagcc caatgatata aataatgttg gtataaaagt caataaaaca aagggaatca
    12661 gcgttccaag aagcactcca gtaattccta tttgatatac aaacaatatc ttcaaagaga
    12721 tagcaactac cgcagttacc actgcaatga atgcttgctg tttaattaaa tccatcccgt
    12781 taagaaaaca gcttattgct aagcctacag attcaaaaag tttccataca aaaagcatat
    12841 aagctagggt atatgtaggt tgtattttat ttttcagcca gagatcaaaa atatcaggta
    12901 aaaaaagaat aatacttcca cctataaata ctgaaactaa caatgccatc ataatgcttt
    12961 tattccaaac atcccgaatc cattttatat ctttctttat ccttgctgat gagtatgctg
    13021 gccataatgg aattaacacc atccccataa ggatcggaat tacactaaac aatcgcacat
    13081 gaacgctata ttcagttaca gcttccgctc caataatcgt atttaagatg atattatcgg
    13141 atgtataagt gcacaccatg gaaatctgta atgcaaaaaa taacacacca gtacttaata
    13201 gtgatttcat ttctgctatt tttataaaac ttatcttgaa aaagccagtt ttacaaatta
    13261 cgagagaaaa gtgcaaatag tttaatagtt gaaatatagc aggtatacca gctattgaaa
    13321 aaaccaacca agccatttca agcttcaagt taataaacag aaacagcaat ccaagtgaaa
    13381 atacactacc taatatttgc catatacttg ctataaaccc caattgatat gccaactgta
    13441 ttttctgtac tatagaaaat agcacattta aactaaagca aaaaacaaat acaaataaag
    13501 catgctctat agaatgtgag tcaatttttt cactcagatt caaaattcta ttccaactaa
    13561 ctaaaggagt ggcagcatag aataataaat tgattgatgt cgcgataatc aataatactg
    13621 taacagcatt tgtgatgatt tttcttatat catttacact attactatat attgcttttg
    13681 ataatttatt tacaactcca ttcccaatcc ccatatctgc aaaagtcaac atcgcaataa
    13741 tggaacttaa tgtcatccaa atcccgaaca attcctcacc taagtaattt aatattgctg
    13801 gaatagttat aaatatagat atcatagaaa taatctttga caacaaagat atgatagcag
    13861 tcagataaac tccacgtaat ctaataactt catctttttc tttctgtgtc aaatctgccg
    13921 ctattggttt aaatataata actatccgat ctatcacatt ttttattttt gacagcatac
    13981 atatctcatt gttgagttat aaagctcaat atagaaccat gataatttat acttttacta
    14041 ttccgacatc cacagctttt tatttttaca aagagtttca ttttaatttt tccacgctac
    14101 cgcccctggc tttacagcta ccag
//

</pre>