LOCUS KJ778809 14124 bp DNA linear BCT 29-MAR-2016
DEFINITION Escherichia coli strain H 19 serotype O84:H21 O-antigen gene
cluster, complete sequence.
ACCESSION KJ778809
VERSION KJ778809.1
KEYWORDS .
SOURCE Escherichia coli
ORGANISM Escherichia coli
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
Enterobacteriaceae; Escherichia.
REFERENCE 1 (bases 1 to 14124)
AUTHORS DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
TITLE Comparison of O-Antigen Gene Clusters of All O-Serogroups of
Escherichia coli and Proposal for Adopting a New Nomenclature for
O-Typing
JOURNAL PLoS ONE 11 (1), E0147434 (2016)
PUBMED 26824864
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 14124)
AUTHORS Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Swimley,M.,
Baranzoni,G.M., Debroy,C. and Liu,Y.
TITLE Direct Submission
JOURNAL Submitted (30-APR-2014) Molecular Characterization of Foodborne
Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
PA 19038, USA
COMMENT ##Assembly-Data-START##
Assembly Method :: CLC Genomics Workbench v. 7.0
Coverage :: >50X
Sequencing Technology :: IonTorrent
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..14124
/organism="Escherichia coli"
/mol_type="genomic DNA"
/strain="H 19"
/serotype="O84:H21"
/db_xref="taxon:562"
misc_feature 1..14124
/note="O-antigen gene cluster"
gene complement(239..1663)
/gene="manM"
CDS complement(239..1663)
/gene="manM"
/codon_start=1
/transl_table=11
/product="phosphoglucosamine mutase"
/protein_id="AJE24481.1"
/translation="MLNVKKIINDSNIAFGTSGARGLVIDFSHDVCAAFTHAFLSVID
DKYNFNKVALAIDNRPSSYEIAQACAYAIKQHGFEVEYHGVIPTPALAHYSMQKNIPC
IMVTGSHIPFDRNGLKFYRPDGEITKEDELAIINSEYTFSPVGVLPHLETSTQGADYY
LERYVSLFNPEILKGKRIGVYEHSSAGRDLYAPLFNQMGAEVISLGRSDEFVPIDTEA
VSDEDRILAREWSKKYNLDAIFSTDGDGDRPLVADENGEWLRGDILGLLTAIELNIKA
LAIPVSCNTAIEESNKFASIQRTKIGSPYVIAAFADLAKQFDSVAGFEANGGFLLASD
LQINGKELKSLPTRDAVLPALMLLIASRNSTISQLINNLPQRFTWSDRVKNFPSDSSQ
QIIKNAISSPNNFFNSLGYESLSCSAIDETDGARFTLNNGDIIHLRPSGNAPELRCYA
EASDENQAKQYVTNVLGNITSLIS"
CDS complement(1669..2415)
/codon_start=1
/transl_table=11
/product="PGL/p-HBAD biosynthesis
glycosyltransferase/MT3031"
/protein_id="AJE24482.1"
/translation="MRISIITATYNSEKTLLDTLLSLEKQTHPDIEYIVVDGASKDNT
IKLIKRNCTKVSKIICEPDKGIYDALNKGIQVASGDVIGFLHSDDLLAYDDAIADIAK
TFESTGCDAIYGDLEYVAQNDTTKRIRLWKSGSFSRFKMKVGWMPPHPSFYMKRECYS
QFGSFSLDYRISADYDSLLRYILKQRISIAYLPKVLVKMRVGGISNRSVSSMVKKSME
DIRIMKQNGIIWPLALAYKNISKLPQFIKK"
gene complement(2415..3821)
/gene="manC"
CDS complement(2415..3821)
/gene="manC"
/codon_start=1
/transl_table=11
/product="mannose-1-phosphate guanylyltransferase 1"
/protein_id="AJE24483.1"
/translation="MLLPVVMAGGSGTRLWPLSRTLYPKQFLSLNSRLTMLQETLRRL
DKVEHKPALVICNESHRFIVAEQLRKESLKHSGILLEPVGRNTAPAVALAALQAMVTG
DDPILLVLAADHEIQDEDNFIAAILAAKNFAEQGKLVTFGIIPTSPETGYGYIKSGES
LDGQGYKVAAFVEKPDLHVAQRYISDGGYLWNSGMFMFRASVFIDELKKFRPDILASC
QRSLSSSIQDLDFIRLDSASFSCCPEESIDYAVMEKTAEAVVVPLNAQWSDVGSWSAL
WEISSKDQSGNAIRGDVLVEDATDSYLYSQHRLIGAVGVKDLIVVETKDAVLVAHKDK
VQQVKNIVAQLKKNNRTEYLQHREIFRPWGSHDTIAEGPRFQVKHVIVLPGHITAKQI
HYHRTEHWIVVSGTAKVHLEDKTYLVSENESTYIPVGVPHAIENPGKIPLEIIEVRSG
VYLEEDDVIRVSSSGVGY"
gene complement(3827..4288)
/gene="gmm"
CDS complement(3827..4288)
/gene="gmm"
/codon_start=1
/transl_table=11
/product="GDP-mannose mannosyl hydrolase"
/protein_id="AJE24484.1"
/translation="MNKRLERELFKTIVEHTPLISIDLIIRNDKGEALLGQRLNRPAQ
NYWFVPGGRIYKDESFEIAFKRITLEELGVQISLNDALFLGVYEHFYNDNFFEAEFST
HYIVHGYEIQLKPQQLHLPTVQHNSYKWFDVVTLLNSTIVHQYTKNYFIPR"
gene complement(4291..5256)
/gene="fcl"
CDS complement(4291..5256)
/gene="fcl"
/codon_start=1
/transl_table=11
/product="GDP-L-fucose synthase"
/protein_id="AJE24485.1"
/translation="MTKKRIYVAGHRGMVGSAICRQLSQRDDIELVVKTHKELDLTVQ
KDVDAFFEQEKIDQVYLAAAKVGGIYANNTFPAEFIYQNLMIESNIIHSAHKAGIQKL
LFLGSSCIYPKFAEQPMNESALLMGILEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
VMPTNLYGINDNFHPENSHVIPALMRRFHEAKESGAPEVIVWGTGTPMREFLYVDDMA
AASVHVMELDEAIYQQNTQPMLSHINVGTGVDCSIREMAETMASVVGYQGKIVFDFTK
PDGTPRKLMDVTRLKNLGWQYRYNLHEGLSLTYKWFIGNINSFRG"
gene complement(5260..6384)
/gene="gmd"
CDS complement(5260..6384)
/gene="gmd"
/codon_start=1
/transl_table=11
/product="GDP-mannose 4,6-dehydratase"
/protein_id="AJE24486.1"
/translation="MKNKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTER
VDHIYQDRHNENPDFILHYGDLTDSSNLIRLIKEIQPDEVYNLGAQSHVAVSFESPEY
TADVDAMGTLRLLEAIRICGLEKKTRFYQASTSELFGLVQEIPQRETTPFYPRSPYAV
AKMYAYWITINYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANVSQGIEKCLY
LGNMDSLRDWGHAKDYVRMQWMMLQQERPEDFVIATGKQISVREFVRMAAKEVGLELQ
FSGKGIDEIATVVNKTSDCAIGVNVGDVIVRVDPRYFRPAEVETLLGDPSKAKKLLGW
EPEITVEEMCAEMVASDLAKAKQHALLKSHGYDVAVSLER"
gene complement(6374..7645)
/gene="mfpsA"
CDS complement(6374..7645)
/gene="mfpsA"
/codon_start=1
/transl_table=11
/product="mannosylfructose-phosphate synthase"
/protein_id="AJE24487.1"
/translation="MKVKIISYSLSKGGAAKAAKNFLHLASKNKKYEAEMISVSGIIK
NGVLFKSSKLSVFWHYSKMLVSRVLTYFILNDKSIKYSLNIFDSNYVLKHIRVEDGQR
EVIHINWVNNDTISLFSLSKLFENKRKRIILTLHDEWFYCASEHYAKYNSLCYVTGYD
DSNFINSYIFELKKRINFENVVITVPSYWLYERAKKSYLLSNCDIYILPNYIDTNIFT
ELKDIRSKRYRILDVPEDAFIIGFGAVSGGANPLKGFDLLVSALDEVLSKINDKARIV
MVTFGANDIDKRVTSLGCHIINMGVISNSHNMAEIYNYLDVMIVPSRVESFGQVAAES
LACKTPVIAFDYSGIKDIVTHQRSGLLAEPFSVDSLALNINSFMNMTEKERYVYGENG
RDDVVRKFGETVVSEIYFKLIDYVRGDEYEK"
gene complement(7642..8763)
/gene="tagF"
CDS complement(7642..8763)
/gene="tagF"
/codon_start=1
/transl_table=11
/product="CDP-glycerol:poly(glycerophosphate)
glycerophosphotransferase"
/protein_id="AJE24488.1"
/translation="MSKKIKYIINFAMSLFILPFFYIEKKIIGKKIWLCGAGNGYYEN
NIASIHDYILKSLPLAKNKIFFVTTNRELLSGDVAFPVLIRGHLKTYALSILADYLIF
DTCNSDIAPGIHRYLSALKVNVNHGFEGLKKLPEDYYAKIDADIHCASSMREKYIKVF
ECGAANEKVFITGYPRFDRIVSRENSHVKKILFFPTWRSWLESLSNDELNNSTYVRSI
YDFLYSERLKEYLSENNIIMYYKPHHKISHLKLDLSSCKNIILLKDYDDLTHYICESD
LLITDYSSVAWDFLYSNREVIFYIFDIDEYILKQGLYYDVRTSLRNYGGTPDEIINLL
KKERKEVDLNLSSMAGEFFEYRDNKNSERILKLICEYKI"
gene complement(8765..9916)
/gene="wzy"
CDS complement(8765..9916)
/gene="wzy"
/codon_start=1
/transl_table=11
/product="O-antigen polymerase"
/protein_id="AJE24489.1"
/translation="MAIYIFISISCLISYVLSNNDKNSLSPRFLLIILTLFTGLSYTN
GWDWYGYLDFYNNIQNEGVSAIYEYNIYGIEYLYLIYLYIVGLSGGSFSLFILLNAII
INFLIYSFCKRAYVNYSLFMFIFIAASYLRLELSTIRQGLAVVIVIYSYSLLLNSKIN
LSLLFIFLAICFHRSAAIVLLFFPFVYYNSARNIHYFIVILTIPFVILSGELNVVFIK
ILNAFNSSLLNAYISKLIIYLSLNKQAIINPQAMALLVFYFICIRVCDVKNKKQIIFL
NIIACQVIISFYFIFLTQLIIMRLVYYFQVGWICWVIILYKEYFRPKWLCFFLICLLF
ITKSILNFRYEQDRAVFFPYYNVISSYLDDNYGRSRSFILNKADELFKE"
CDS complement(9918..11057)
/codon_start=1
/transl_table=11
/product="glycosyl transferases group 1"
/protein_id="AJE24490.1"
/translation="MLKVGIPVLFSEQHWMGGINYFRSLISAFSLVEQNDIELIIFCE
KDGLFNTTKLNNVRQVIIPELLPRKLSKRIINKVYGVNFELYKAIKNESVDILSHTQI
NKRLACKTMWWKPDFQEKYYPEFFNEKDLFLRNQSVVNNALRGYLLFSSNDAKNDFFK
FYNTLPTQNINVLQFVPEIEISSSQDENDRIIAVKEKYGISGDYFFLPNQFWKHKNHE
LVIKAIITGDIPAKVVATGASNDYRGGRHIELINQLLKQDSAKKFKLLGLVDRDDVNL
LMKGAIAVINPSRFEGWSTTVEEAKYLGKRLILSDIPVHHEQNPPDSLYVSCDDVSGM
ISAMRKIIDEYDTEKEIFRAKKAAEQYKFKRKEFGLSYYNILKSL"
gene complement(11051..11953)
/gene="rmd"
CDS complement(11051..11953)
/gene="rmd"
/codon_start=1
/transl_table=11
/product="GDP-6-deoxy-D-mannose reductase"
/protein_id="AJE24491.1"
/translation="MILGAGGFIGSHLCNSCIQNGATVYALGRNLDNVKIKNQRFIKL
SSNEITEKTLFDVAQPDYVYFLMGAASVAKSIEAPLYDFNQSLPPLLVILEKLRKEWI
GTRLIFISSAAVYGENASERTSVSSLPMPLSPYGLNKKISETYVQYYMKHYDISAKII
RPFSVYGPGLKKQLIWDVLNKIKDKQHHYFGSGNEMRDWIYIDDFIAVLFNYVSNYAL
MSDLINVGTGIPIKVKNVIEQLYTLTDTFEIPSFSDGGKEGDPKHLVSNYEEQDFLRE
LLKTPLELGLRNTVDWFKNQEEQC"
CDS complement(11973..12584)
/codon_start=1
/transl_table=11
/product="galactoside O-acetyltransferase"
/protein_id="AJE24492.1"
/translation="MKKFISRLLYNPYKKIKKDSRIEFNSGILDKNFRVHFYCSESRA
KAYIGNNCILKNEIIFERECGVVTIGDNTFINKNTKIISVNRISIGSNVTISYGVIIY
DHDSHSLDYRERQKDILKILETRDPNNDIENKNWDCVKSLPVIIGDNVWIGFQAAILK
GVTIGEGAVVAARAVVTKDVPAWSVVAGNPAKVVKEIPRDMRK"
gene complement(12584..13978)
/gene="wzx"
CDS complement(12584..13978)
/gene="wzx"
/codon_start=1
/transl_table=11
/product="O-antigen flippase"
/protein_id="AJE24493.1"
/translation="MLSKIKNVIDRIVIIFKPIAADLTQKEKDEVIRLRGVYLTAIIS
LLSKIISMISIFITIPAILNYLGEELFGIWMTLSSIIAMLTFADMGIGNGVVNKLSKA
IYSNSVNDIRKIITNAVTVLLIIATSINLLFYAATPLVSWNRILNLSEKIDSHSIEHA
LFVFVFCFSLNVLFSIVQKIQLAYQLGFIASIWQILGSVFSLGLLFLFINLKLEMAWL
VFSIAGIPAIFQLLNYLHFSLVICKTGFFKISFIKIAEMKSLLSTGVLFFALQISMVC
TYTSDNIILNTIIGAEAVTEYSVHVRLFSVIPILMGMVLIPLWPAYSSARIKKDIKWI
RDVWNKSIMMALLVSVFIGGSIILFLPDIFDLWLKNKIQPTYTLAYMLFVWKLFESVG
LAISCFLNGMDLIKQQAFIAVVTAVVAISLKILFVYQIGITGVLLGTLIPFVLLTFIP
TLFISLGFLRKGMC"
ORIGIN
1 catcactgcc ataccgacga cgccgatctg ttgctttgac attgtttact cctgtcaggg
61 ggtgttcacc gcgatgtatg cgcgacttga atatgattga gatgttatct caagttgcac
121 gaatgtgtgt agtgctgaat ttcattaatg caacaaatac cgttcttcaa ttctgtatac
181 cggcatctac gtgtaatcaa acctacgcac acatataagc gtagataaac ctataacatc
241 aagaaatcaa agaggtaata tttcccagca cattcgtaac atattgctta gcctgatttt
301 catcactggc ctcagcataa caacggagtt ctggagcatt accggaagga cggagatgta
361 taatatcacc attatttaaa gtaaatcttg caccatccgt ttcatcaata gcggaacagg
421 ataatgattc ataacctaaa ctattaaaga aattattggg tgacgatatg gcatttttta
481 taatttgttg acttgaatct gaagggaagt ttttaactct atctgaccaa gtgaatcgct
541 gaggaagatt attaatcagt tgagaaatag tactattgcg agaagctatt aagagcatta
601 atgctggtaa cacagcatct cgtgtaggta acgattttaa ttccttgcca ttaatttgta
661 aatcagaggc aaggagaaaa ccaccattag cttcaaaacc agcgactgaa tcaaattgct
721 tagcaagatc tgcaaatgct gcaattacat aaggagaacc tattttcgtt cgttgtatac
781 ttgcaaattt attagactct tcaattgctg tattacaact cactggaata gccaacgcct
841 tgatattaag ttcaatagca gtaagtagtc ccagaatatc gcctcttaac cattcaccat
901 tttcatcggc aactaaagga cgatcaccat cgccatctgt tgagaaaata gcatcaagat
961 tatatttttt agaccactct cttgcaagta tacgatcttc atcgcttact gcttccgtat
1021 caatcggaac gaactcatca ctcctaccga gggaaatgac ctctgcaccc atttgattaa
1081 aaagaggagc atataaatcg cgtcccgcac tagaatgttc atatacccct attcttttcc
1141 cctttaaaat ctcgggatta aaaagagaaa cataacgctc gaggtagtag tctgcacctt
1201 gagtgcttgt ttcaagatga ggtaatacac ctacaggcga aaatgtatac tcactattta
1261 taattgcgag ctcatcctct ttagtgattt caccatcagg tctgtagaat tttaagccat
1321 tacgatcgaa agggatatga ctccctgtga ccattatgca gggaatgttt ttctgcatcg
1381 aataatgagc taatgcagga gtcggaatta caccatgata ttcaacctcg aatccatgtt
1441 gttttatagc atacgcgcat gcctgagcaa tttcgtaact acttggacgg ttatcaatgg
1501 ctaaggcaac tttattaaaa ttgtatttgt catcaataac agaaagaaac gcatgagtga
1561 acgcagcaca aacatcatgg gaaaaatcga tcactaaacc gcgagcacca ctagtaccga
1621 acgcaatatt actatcgtta atgatttttt taacatttaa catgatgatt actttttaat
1681 aaattgagga agtttggata tatttttata cgctaaagca agtggccaga taataccatt
1741 ctgtttcata atacgaatgt cttccattga cttcttgacc attgaagata ctgaacgatt
1801 gctaattcca ccaacacgca tcttcactaa gacttttggt aaatatgcta tcgaaatacg
1861 ttgttttaaa atatatcgta ataatgagtc ataatcagca gatattcgat aatctaaaga
1921 aaaactacca aactgactat aacattcgcg tttcatataa aatgatgggt gtggtggcat
1981 ccaacccact ttcatcttga aacgactaaa tgaaccactt ttccataatc taatacgttt
2041 agtcgtatca ttttgggcaa catactccaa atcgccataa atagcatcac accctgtact
2101 ttcaaatgtt tttgctatat ctgcaatagc atcatcataa gctagtaaat catcagaatg
2161 taaaaagcca ataacatcac ccgaagctac ttgaatacct ttattcagag catcataaat
2221 gcccttatcg ggttcgcaaa tgatttttga aacttttgta caattcctct tgatcagctt
2281 aattgtatta tctttcgatg ctccatctac aactatatat tcgatatctg gatgcgtttg
2341 tttttctaga gaaagtaatg tatcaaggag tgttttttca ctattataag tggctgtgat
2401 aatagaaatt cgcattagta tcctactcca gaagaagaaa ctcttataac atcatcctct
2461 tccaggtaga ctcctgatct aacctcaatt atttcaagcg ggatcttgcc aggattttca
2521 atggcatgtg gaacaccaac aggtatatat gttgattcat tttcagaaac aaggtaagtc
2581 ttatcttcaa gatgaacttt agctgtcccc gagacaacaa tccaatgttc agtacgatgg
2641 taatgaatct gcttagcggt aatgtgacca ggcaatacaa tcacatgttt cacttgaaag
2701 cgcggccctt cagctatagt atcatgactg ccccaaggcc gaaatatttc ccgatgctgt
2761 aaatattctg tgcgattatt ctttttaagt tgagcgacga tgtttttaac ttgctgaact
2821 ttatctttat gagcaactaa tacggcatct ttcgtttcaa caacaatcaa atcctttacg
2881 cccacggcac caataagtct atgctgcgaa taaagataac tatctgtagc atcttcaacc
2941 aatacatcac cacgaatggc attaccgctt tgatcttttg aacttatttc ccacaatgca
3001 gaccatgacc caacatcact ccattgtgca tttaatggaa cgacgacagc ttcggctgtt
3061 ttttccataa ctgcatagtc aatagactct tcaggacagc aagaaaatga agcactatcc
3121 agacggataa aatctaaatc ttgtatcgaa gaggacaagg agcgttggca actggctaaa
3181 atatctggtc ggaatttttt cagttcatcg ataaatacag atgctctgaa cataaacatc
3241 ccgctattcc aaagataacc gccatctgat atgtaccgct gagctacgtg aagatctggt
3301 ttttcaacaa aagccgcaac tttgtaacct tgtccatcca gagattcacc tgacttaatg
3361 taaccatagc cagtctctgg ggatgttgga ataataccaa acgtaacaag cttaccctgc
3421 tctgcaaaat tctttgcagc gagaattgca gcaatgaaat tatcctcatc ctggatttca
3481 tgatcagcag caagaaccaa aagaatagga tcgtctccag ttaccatagc ctgaagtgct
3541 gcaagtgcta cagcaggcgc agtattacga ccaacaggct caagcaaaat accgctatgc
3601 tttaaactct ctttacgcaa ttgttcagca acgataaagc gatgtgattc gttacaaatg
3661 accaaagcag gtttatgttc gaccttatca agccgccgca atgtctcttg caacatggtt
3721 aaacgactat ttaaagacag aaattgtttc ggataaagtg tacgtgaaag aggccataat
3781 ctggtaccag aaccaccggc catgacaacg ggaagtagca tatctattac cttggtataa
3841 aatagttttt ggtatattga tgaactatag tgctattaag caacgttact acatcaaacc
3901 acttgtagga attatgctgg accgttggta ggtgaagttg ctgaggttta agttggattt
3961 catatccatg cactatatag tgtgtagaaa attctgcttc aaaaaaatta tcattgtaaa
4021 aatgttcata caccccaaga aataaggcat cattaagact aatttgaacg cccaactctt
4081 caagtgttat ccgcttaaat gcaatctcga atgactcatc cttataaatt cgccctcctg
4141 gcacaaacca ataattttgt gctgggcgat tcaggcgctg ccccagcagc gcctctcctt
4201 tatcgtttct aattatgaga tcaatcgaga ttagaggagt atgctcaact attgttttaa
4261 atagctcacg ttccaatctc ttgttcataa ctatccccga aaagaattaa tattcccaat
4321 aaaccattta taagttaatg ataagccttc atgtaaatta tagcgatatt gccagcccaa
4381 gtttttgagc cgggtaacgt ccataagttt acgcggagtg ccatcaggtt tagtaaaatc
4441 aaaaacaatt ttaccttgat aacccaccac agaggccatt gtttcagcca tttcacgtat
4501 agaacaatct acacccgtac caacattaat atgggataac ataggctgtg tattttgttg
4561 ataaattgct tcgtcaagct ccattacatg gacggatgcg gcagccatat catcaacata
4621 taaaaactca cgcatcggtg ttccggttcc ccagacaata acctctggcg caccactctc
4681 ttttgcttca tggaatctac gcatgagtgc cggaataaca tgagagtttt caggatggaa
4741 attatcattt atgccataaa gattagtagg cattacactg cgataatcac ggccatattg
4801 gcggttataa gattcacata attttatgcc cgcaatttta gcaattgcgt atggttcatt
4861 agttggctca agtatgccca ttaaaagcgc tgactcgttc atcggctgct ctgcgaattt
4921 aggataaata cagcttgagc ctaaaaaaag taatttttga attccggcct tgtgagctga
4981 atgaataata ttactctcaa tcatgagatt ctgatagatg aattctgccg gaaatgtatt
5041 gttggcataa atgccaccaa ctttagccgc agcaagataa acctgatcaa ttttctcttg
5101 ctcaaaaaat gcatcaacat ccttctgtac ggttagatcg agttctttgt gtgttttgac
5161 cactaattcg atatcatcac gctgtgataa ttgacggcaa atagcagagc caaccatacc
5221 tcggtgacca gcaacgtaga tacgtttttt tgtcatacct taccgctcca gagaaactgc
5281 aacatcgtaa ccatggcttt tcagtagtgc atgctgtttt gctttcgcca gatcactggc
5341 aaccatttcc gcacacattt cttcaacagt aatttctggt tcccaaccca ataatttttt
5401 agctttactt ggatcaccaa gaagtgtttc aacttccgca gggcggaaat agcgtggatc
5461 aacacgaaca attacatccc caacatttac gccaatagca cagtcagatg ttttgtttac
5521 aacagtagct atttcatcaa tacctttacc agagaactga agttccaagc caacttcttt
5581 agccgccatt cggacaaatt cacgtacaga aatttgttta ccggtagcga ttacaaaatc
5641 ttccgggcgt tcttgttgaa gcatcatcca ctgcatacgg acataatctt tagcatgtcc
5701 ccagtcacgc aatgagtcca tgttaccaag atagagacat ttttcgattc cttgcgaaac
5761 attagcaatt gcacgagtaa ttttacgtgt aacaaatgtt tcgccacgac gtggagattc
5821 gtggttgaac aagataccgt tacaggcata cataccataa gattcacgat agtttatagt
5881 aatccagtat gcatacatct tagcaacagc ataaggcgaa cgaggataga acggtgtagt
5941 ttctcgctgc ggaatttctt ggactaagcc gaagagttca gaagttgatg cctgatagaa
6001 acgcgtcttt ttctctagtc cacaaatacg gatcgcttcc agcagacgta aagtccccat
6061 agcatctaca tcagcagtat attcaggtga ttcaaatgaa actgctacat gagattgtgc
6121 tccaaggtta taaacttcat ctggttggat ttctttaatc aagcgtatca ggtttgatga
6181 gtccgttaag tcaccataat gaagtatgaa atctgggttt tcattatgac gatcctgata
6241 gatatgatct acacgttcag tattaaaaga agatgctcgg cgttttatac cgtgcacttc
6301 ataacccttt tcaagaagaa attctgccaa gtaagaacca tcttgaccgg taacaccagt
6361 aattagagct actttatttt tcatattcat ctcctctaac ataatctatc aatttaaaat
6421 aaatttctga aaccaccgtt tctccaaatt ttcgaactac atcatcgcga ccattttcac
6481 catatacata tctctctttc tccgtcatat tcataaatga atttatattt agtgcgagtg
6541 aatcaacaga aaatggttca gctaataaac cactccgttg atgagtaaca atatctttaa
6601 ttcctgaata atcaaatgca ataaccgggg ttttacaagc taaactttca gcagctactt
6661 gcccaaagga ttcaacgcgt gaaggaacaa tcatgacatc caaataatta taaatctcag
6721 ccatattatg gctgttagag ataactccca tatttataat gtggcatccc agtgatgtta
6781 cacgtttatc tatatcgtta gcaccgaacg tcaccattac aattctagct ttgtcattta
6841 ttttacttaa tacttcatcc aaggcagaaa ccaagagatc aaaccccttc aaggggttgg
6901 caccgccact aacagcacca aatccaataa taaaagcatc ctcaggaaca tcaagtattc
6961 tataacgttt tgaacgaatg tcctttaact ctgtaaaaat attagtatcg atataattag
7021 gcaatatata tatatcacaa ttacttaaca aataactttt ctttgctcgc tcatacaacc
7081 aatatgatgg aacggtaatt acgacattct caaaatttat tctctttttt aattcaaaaa
7141 tatagctatt aataaaattg ctatcatcat atcctgttac ataacataat gaattatatt
7201 tagcatagtg ctctgacgca caataaaacc attcatcatg taacgttaat attattcttt
7261 ttcttttatt ttcaaataat ttacttaatg aaaataaaga tatggtatca ttattcaccc
7321 aattaatatg gataacttcc ctttgcccat cttccactct gatatgtttt aaaacataat
7381 tagagtcaaa aatattcaat gaatatttta tacttttatc atttaaaata aaataagtga
7441 gtacccggct tactaacatt tttgagtaat gccaaaacac tgataatttt gatgatttaa
7501 ataaaacacc attttttata atacctgata cacttatcat ctctgcttca tattttttgt
7561 ttttcgaagc aagatgtaaa aaattttttg ctgcttttgc cgctccgcct ttggataaag
7621 aataagaaat tatttttact ttcatatctt atattcacag attaatttta gaattcgctc
7681 agaattttta ttatctcgat actcaaaaaa ttcccctgcc atacttgata aatttaaatc
7741 aacttcctta cgttcttttt taaggagatt aataatttca tcaggtgtgc cgccataatt
7801 tcttaaggaa gttctgacat catagtataa accttgtttt aatatatatt catcaatatc
7861 aaatatatag aaaattactt ctctattaga gtaaaggaaa tcccacgcaa cgcttgaata
7921 atctgtaata agcaaatctg attcacatat ataatgtgta agatcatcat aatcttttag
7981 caaaataata tttttacaag aagataaatc taatttcaaa tggctaattt tatgatgtgg
8041 cttataatac attataatgt tattttcact caaatattct ttgagtctct cactgtatag
8101 aaaatcataa atagatctga catacgtgga attatttaac tcatcattgc ttagactctc
8161 caaccatgat ctccatgttg gaaagaaaag aatttttttc acatgactgt tttcacgtga
8221 aacgattcga tcaaatctag gataacctgt aataaatact ttctcattcg ctgctccaca
8281 ttcaaatacc ttaatatatt tctctctcat tgaagaagca caatggatgt cagcatcaat
8341 tttcgcataa tagtcttcag gaagcttctt taagccttca aagccatgat taacatttac
8401 cttcaaagca gaaagatacc ggtgaattcc aggagcaata tctgagttac aagtatcaaa
8461 gattaaataa tcagctaaaa tactcaacgc ataagttttt aaatgccctc taataagtac
8521 aggaaaggct acatcaccgc ttaaaagttc ccgatttgtt gttacaaaaa aaattttatt
8581 tttagctaaa ggtaaactct tcaaaatata atcatgaatg ctagctatgt tattttcata
8641 atagccattt cctgcgccac ataaccaaat tttttttccg attatctttt tttctatata
8701 gaaaaatggt aatataaaaa gtgacattgc aaaatttata atatacttaa ttttctttga
8761 cattttactc cttaaaaagc tcatctgctt tattcaatat aaaagatcta cttctcccat
8821 aattatcatc caggtatgaa gatattacat tataatatgg aaaaaaaacc gctctatctt
8881 gctcatatct aaaatttaat attgacttgg taataaacag aagacatata aggaaaaaac
8941 ataaccattt aggtcggaaa tactccttgt ataatattat tacccaacat atccatccaa
9001 cttgaaaata ataaaccaga cgcataataa tcaattgtgt taaaaatata aaataaaaag
9061 aaattataac ctgacaagca attatattta aaaatataat ttgttttttg tttttaacat
9121 cacaaaccct gatacaaata aaataaaaga ctaataatgc catagcttgt gggttaataa
9181 ttgcttgttt gtttaatgac aaataaatta taagcttcga tatatatgca tttaataatg
9241 atgaattaaa agcattcaat atttttataa atacaacatt taattccccc gaaagaataa
9301 caaatggtat agtaagtatg acaataaagt aatgaatatt tcttgcacta ttataataaa
9361 caaacggaaa gaataataat actatagcag cgcttctatg aaagcaaata gccagaaata
9421 taaacaataa ggataaattt atttttgagt ttaataaaag cgaatatgaa tatataacta
9481 taacaacggc caatccttga cgtatagtac ttaattccaa cctcaaatat gatgcagcta
9541 taaaaataaa catgaacaag ctataattaa cataagctcg tttgcaaaaa ctataaatga
9601 gaaaatttat tattattgca ttcaataata tgaataagct aaaacttcct ccacttaggc
9661 caacaatata caaatatatt aaatataaat actcaatgcc atatatatta tactcatata
9721 ttgcacttac cccttcattt tgaatgttat tatagaagtc aagataaccg taccaatccc
9781 atccatttgt ataagataac cccgtaaata aagttagtat tattaacagg aatcttggac
9841 tcaatgaatt cttatcatta ttcgacaaaa catatgatat taaacatgaa atgcttataa
9901 atatatagat ggccatatta tagtgacttt aagatattat agtatgagag accaaattcc
9961 tttcttttaa acttatattg ctcagcagcc ttctttgcac gaaatatttc cttctctgta
10021 tcatattcat ctattatctt gcgcatagca gaaatcattc ctgatacatc atcacatgat
10081 acatataatg aatcaggagg attttgttca tggtgtactg gtatatcaga taatattaat
10141 cttttaccta aatattttgc ctcttcgacc gtcgtactcc acccctcaaa ccgagatggg
10201 ttgattacgg ctatagcacc tttcatcaaa agattaacat catccctatc aactaaccct
10261 aataacttga attttttagc agaatcttgt ttaagtaatt gattgattaa ttctatatgc
10321 ctccctccac gataatcatt agatgcccca gttgccacga ctttagcagg tatatctcct
10381 gtaataatgg cctttatcac taattcatgg tttttatgtt tccagaattg gtttggtaaa
10441 aaaaaataat ctccactaat tccatatttt tcttttactg ctattattct atcattttca
10501 tcttgtgaac tgcttatttc aatttctgga acgaattgaa gcacattaat attttgtgtt
10561 ggcaaagtat tatagaactt aaaaaaatcg ttttttgcat cattactact aaacagcaga
10621 tatccacgta aagcattatt aactacagac tgatttcgta gaaataaatc tttctcatta
10681 aaaaattcag gatagtattt ctcttgaaaa tcgggcttcc accacatggt tttgcaagct
10741 aaccgtttat ttatctgggt atgagataaa atgtcaacac tttcattttt aatagctttg
10801 tataattcaa aattcactcc ataaacttta tttattatac gtttagacaa ttttctagga
10861 agaagctcag gaataattac ctgtcgaaca ttattaagtt tggttgtatt gaacaatcca
10921 tctttttcac aaaaaattat aagttcaata tcattttgtt caacaagtga aaaagcagaa
10981 attagacttc taaagtagtt gatccctccc atccaatgct gctcactgaa caatacagga
11041 ataccgactt ttaacattgt tcctcctggt ttttaaacca atcaactgta tttctcaatc
11101 ccaattcaag aggcgttttt aaaagttctc ttaaaaaatc ttgctcctca taattactta
11161 ccaagtgctt tggatcgcct tccttaccac catcactaaa cgagggaatt tcaaatgtat
11221 cggtcaatgt gtacaattgt tcaataacat ttttaacctt tattggaata cctgtaccta
11281 catttatcag gtcactcatc aaagcataat tactaacata attaaacaaa acagcaataa
11341 aatcatcaat atatatccaa tcacgcattt cattaccgct accaaaataa tgatgctgct
11401 tatcttttat tttattcaat acatcccaaa taagttgctt ctttaaacca ggcccataga
11461 cagaaaaagg tcttattatt ttcgcactaa tatcatagtg tttcatataa tattgcacat
11521 acgtttctga gattttctta tttaatccat aaggggataa aggcataggt aaactactta
11581 cacttgttct ttcactcgca ttttcaccat atactgcagc acttgagata aaaatgaggc
11641 gcgtccctat ccattctttt cgtaattttt ccaaaatcac caatagtggg ggtaaactct
11701 ggttaaagtc atataatgga gcttctatag attttgctac agatgccgcg cccattaaaa
11761 aatacacata atctggttgt gcaacatcaa ataatgtctt ttcagtgatt tcatttgaac
11821 ttaattttat aaacctctgg tttttgatct tcacattatc taagttacgc ccaagtgcat
11881 aaactgtagc tccattttgt atacatgaat tacacagatg agaaccaatg aatccacctg
11941 cccccaaaat cattatagtt atatttttca acttacttcc tcatatctct tggtatttct
12001 ttcacaacct tcgctggatt gcctgccacg acagaccacg ccggaacatc ttttgtaact
12061 accgctcttg cagcaacaac cgcgccctca cctatagtaa caccttttag tatcgcagct
12121 tgaaaaccta tccaaacgtt atcacctata attactggta aacttttaac acaatcccaa
12181 tttttatttt ctatatcatt atttggatct cgagtctcta atattttcag aatatccttt
12241 tgcctttccc gataatctag cgagtgtgaa tcatggtcat atattattac accataagat
12301 atggtaacat tacttcctat actaattcta ttaacactta taatcttcgt gtttttgtta
12361 atgaatgtat tatcaccaat agtgacgaca ccacactctc tctcaaatat aatctcattt
12421 tttaggatgc aattatttcc gatatatgcc ttagcacgac tttcgctaca gtaaaaatgc
12481 acacggaaat ttttatccaa aatcccagaa ttaaattcaa ttcgagagtc tttttttatc
12541 tttttgtaag gattatagag caatcttgaa ataaactttt tcattagcac attccttttc
12601 tcaaaaagcc caatgatata aataatgttg gtataaaagt caataaaaca aagggaatca
12661 gcgttccaag aagcactcca gtaattccta tttgatatac aaacaatatc ttcaaagaga
12721 tagcaactac cgcagttacc actgcaatga atgcttgctg tttaattaaa tccatcccgt
12781 taagaaaaca gcttattgct aagcctacag attcaaaaag tttccataca aaaagcatat
12841 aagctagggt atatgtaggt tgtattttat ttttcagcca gagatcaaaa atatcaggta
12901 aaaaaagaat aatacttcca cctataaata ctgaaactaa caatgccatc ataatgcttt
12961 tattccaaac atcccgaatc cattttatat ctttctttat ccttgctgat gagtatgctg
13021 gccataatgg aattaacacc atccccataa ggatcggaat tacactaaac aatcgcacat
13081 gaacgctata ttcagttaca gcttccgctc caataatcgt atttaagatg atattatcgg
13141 atgtataagt gcacaccatg gaaatctgta atgcaaaaaa taacacacca gtacttaata
13201 gtgatttcat ttctgctatt tttataaaac ttatcttgaa aaagccagtt ttacaaatta
13261 cgagagaaaa gtgcaaatag tttaatagtt gaaatatagc aggtatacca gctattgaaa
13321 aaaccaacca agccatttca agcttcaagt taataaacag aaacagcaat ccaagtgaaa
13381 atacactacc taatatttgc catatacttg ctataaaccc caattgatat gccaactgta
13441 ttttctgtac tatagaaaat agcacattta aactaaagca aaaaacaaat acaaataaag
13501 catgctctat agaatgtgag tcaatttttt cactcagatt caaaattcta ttccaactaa
13561 ctaaaggagt ggcagcatag aataataaat tgattgatgt cgcgataatc aataatactg
13621 taacagcatt tgtgatgatt tttcttatat catttacact attactatat attgcttttg
13681 ataatttatt tacaactcca ttcccaatcc ccatatctgc aaaagtcaac atcgcaataa
13741 tggaacttaa tgtcatccaa atcccgaaca attcctcacc taagtaattt aatattgctg
13801 gaatagttat aaatatagat atcatagaaa taatctttga caacaaagat atgatagcag
13861 tcagataaac tccacgtaat ctaataactt catctttttc tttctgtgtc aaatctgccg
13921 ctattggttt aaatataata actatccgat ctatcacatt ttttattttt gacagcatac
13981 atatctcatt gttgagttat aaagctcaat atagaaccat gataatttat acttttacta
14041 ttccgacatc cacagctttt tatttttaca aagagtttca ttttaatttt tccacgctac
14101 cgcccctggc tttacagcta ccag
//