LOCUS AB812034 15306 bp DNA linear BCT 13-MAY-2015
DEFINITION Escherichia coli genes for O-antigen biosynthetic locus, partial
sequence, strain: H14.
ACCESSION AB812034
VERSION AB812034.1
KEYWORDS .
SOURCE Escherichia coli
ORGANISM Escherichia coli
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
Enterobacteriaceae; Escherichia.
REFERENCE 1
AUTHORS Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
Hayashi,T. and Thomson,N.R.
TITLE A complete view of the genetic diversity of the Escherichia coli
O-antigen biosynthesis gene cluster
JOURNAL DNA Res. 22 (1), 101-107 (2015)
PUBMED 25428893
REMARK DOI:10.1093/dnares/dsu043
REFERENCE 2 (bases 1 to 15306)
AUTHORS Iguchi,A., Iyoda,S. and Ohnishi,M.
TITLE Direct Submission
JOURNAL Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
Miyazaki, Miyazaki 889-2192, Japan
FEATURES Location/Qualifiers
source 1..15306
/organism="Escherichia coli"
/mol_type="genomic DNA"
/strain="H14"
/db_xref="taxon:562"
/note="serogroup:O82"
gene <1..885
/gene="wcaM"
CDS <1..885
/gene="wcaM"
/codon_start=1
/transl_table=11
/product="colanic acid biosynthesis protein"
/protein_id="BAQ01260.1"
/translation="GFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIER
INCTNDKINWGIGIGLSGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVI
RNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSI
PQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMR
NINVMQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRIN
HHIVNVEKINFRLPERRE"
gene 1048..1953
/gene="galF"
CDS 1048..1953
/gene="galF"
/note="O082_01"
/codon_start=1
/transl_table=11
/product="UTP-glucose-1-phosphate uridylyltransferase"
/protein_id="BAQ01261.1"
/translation="MDEIMTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
VDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVT
IMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNE
TGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGR
YVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQA
FVKYGLRNLKEGAKFRKGIEKLLSE"
CDS 2476..3999
/note="O082_02"
/codon_start=1
/transl_table=11
/product="putative asparagine synthetase"
/protein_id="BAQ01262.1"
/translation="MCGFFITNNPSINSSHLKLVEDSLAFRGPDFSSGFINTNGWIVY
HSRLSIIDLTEDANQPIFDSKGGVLVFNGEILNFKELGIKYFNKDFESDSKLLSALLE
RKLIDLNEFDGFFSFVYVNHNGKLEYAVRDKFGVKPLFYHKSEEGYLSFSSEPSLLNR
LYNCGLNKDAIDEYYSIRYPIFSGSYFNDVFCINPGECYINGEYFNILNYFSGKYVET
NINEIELSIKKGIKTRKVSDAPVGLLLSKGIDSNLLNTLSEFNFKYSIGFKGDADLEY
LKQQNIKNLKIVECNNQLYKDAFDYLIKLRGEPMSVPNEVLLYLIAKEAASDGVKVLL
SGEGADEFFGGYDRIFTWALYAQEFVLEDFIEHYCYELPAENTKLVYKFKSIFEQVSH
LTPFEKVRWFFIRYHMPILFRRLDFSLMAAGIEGREPLANIHTFMQAIKVSPSQLMTD
GLGKIPLRNIIAKYMGKDFAFSSKVGFPVDLRKVFDYTGNETSYEIWFKENLKGVGI"
CDS 3996..4415
/note="O082_03"
/codon_start=1
/transl_table=11
/product="putative glycerol-3-phosphate
cytidyltransferase"
/protein_id="BAQ01263.1"
/translation="MIIGYTTGVFDLFHIGHVNLLKNAKSLCDKLIVGVTIDELVLYK
GKRSVIPFHERIQVVQACKYVDVALPQSDMDKLTAVKKYHANILFVGDDWYNTEKWCV
YEKELAEIGCKIIYFPYTQGTSSTLINDTLKKLRNNQ"
gene 4475..5704
/gene="wzx"
CDS 4475..5704
/gene="wzx"
/note="O082_04"
/codon_start=1
/transl_table=11
/product="O-antigen flippase"
/protein_id="BAQ01264.1"
/translation="MFKHSVFINATALFFMQLLGYISPLLVYPYLTRVLGISGFGQYA
MAISITALSFIITDFGFALSGANWLAKNRNKTKEVKHYLSNVCLLKFIFFALCSVVSS
LVIITSQQLSYLLHSIPAIIFLLFSQTFQMNWLFQGLEKMSLAIYPFVISRIIFILTV
FIFVKYKADVNIAIFLLGFSNLLSVIISACLLAREGLSFCKPSFRDSFNILKNNISFF
LSRASVSIYTSASAFILGYTTNVHAVAYYSAAEKLYTAGQSLTGTVSQALYPYLSRTG
NKNIFNIALIIVMLPLTIGIGIAFIFSSELINLIYGTAYSESIPILKIFLICLIINFI
GVSYGYPAFAIINRIDVANKTVYMGGLFQISSLLYLYVNDIISANSVAISVLMTETFV
MCLRILFYYIFSKNAHD"
CDS 5697..6875
/note="O082_05"
/codon_start=1
/transl_table=11
/product="putative glycerophosphotransferase"
/protein_id="BAQ01265.1"
/translation="MIKKLKLKIYALSSYLLGHFLNADDDLWIIGAGNGYVFGDNSRY
FYQWVIEKKNKKIFWVTKDLKIYHALSKNEKTKDRVIYFYSLKSILFIARAKVFICSH
GVNDITPYVCKNRKLICLWHGLPIKKIGDLTLSSYGKDNPLERGIKHVINSKKEYDVF
LSPSLYYKEIFEKSFEGKISEFIYAQYPRVTELKNKSNDIADNSDIKTILYAPTFRDF
VAPSYYEINGIVPSKKLLSELNCILGYNRYKLIIKLHPYICIENSYMHELIGFNNIIF
ANKNDDVLDLLINASVLITDYSSICFDFLTLGKEIIFLMPDYDQYVGQTRGLNFELKD
FAPGKIFTNWNDLIKYIPVINRKFTLDDDYLKALKMSNEFHTRKNSYIFDFIEKRAKL
"
gene 6875..7957
/gene="wzy"
CDS 6875..7957
/gene="wzy"
/note="O082_06"
/codon_start=1
/transl_table=11
/product="O-antigen polymerase"
/protein_id="BAQ01266.1"
/translation="MVKEFKIKTDCYIIILWFFIAAFKILFNNILYFRDVEVIQFIIT
ILFWLIGLVIILRRFLNHAIKLDTLLIVCSVLICLSILNANLLTFVFAVLVWSSISYL
LDRRAFIFFYSASLFFLIITILISVALNEQVFYIDGRYGKVPTFGFENSNSFPQLLVI
YLLISTSRLRTSIILGSIILFFAYMELKTRSMYMIIILYPLVILLLKFFKGKIICILP
LVLIVLNFVMVLNLNNEYIKSLDVLLSYRLSYSLELINNLSFSQWFTGTSSELGIPMD
MSYINLVYKYGIIATAMIVVLAMKALHVALSHSDSKVVAIIVCFFCYAFVENVLLNYY
LNPTLYFIFYFYAKNKQREWLNNNAG"
CDS 7981..8670
/note="O082_07"
/codon_start=1
/transl_table=11
/product="putative glycosyltransferase"
/protein_id="BAQ01267.1"
/translation="MIPKIIHYIWLGDIDNMPKLSKKCIDSWKKHLLNYEIIFWDECK
IRSTFKENEIEFFNKMVKLKKFAFAADYARCIILDKFGGVYLDTDIEIIRDITPLLSV
SDVFLGLEDDNKPNCAVIGATSNNIFLKKLIKFIIQADGLTEIPILAYEALLGISERF
CDLNRLTIIDGIAVYPEQFFYPYNPYRRDSLSQLMFSDITEETYAIHHWAKTWKLTIF
ERIKKRLFKQK"
CDS 8855..9745
/note="O082_08"
/codon_start=1
/transl_table=11
/product="putative glycosyltransferase"
/protein_id="BAQ01268.1"
/translation="MSRVTIGILTYKRMDYLISLIKDLNAVKIKIDLIILNNNENIDV
FNDIADIVDSNFINYKYIWNQKNHGVSEGRRIIVENCNTDYLMLLDDDIYVEDINLIC
RKIINSFDSDKFLGAIAFNIIDFNTKKNNRYEIPHKNKKVNLNADFYTYLIIGAGLAL
RTSAVKDVGNFSNTLGPYGFEEIDVAFRLINAGYKIKYLHDCIIEHKKSPDGRFSNEM
VNYYALVNRTILAKKYLRKKYFISCLVIRSLFFIVKTKNIRLLFKAWAEIIKTESHAS
DKFKQCFYDYCKGVSGFLYY"
CDS 10069..10809
/note="O082_09"
/codon_start=1
/transl_table=11
/product="putative glycosyltransferase"
/protein_id="BAQ01269.1"
/translation="MPYVTIVMPAYNSEMYIAQAIESVINQTHTNFELLICDDGSTDG
TTSIVSKYIVQDNRIKLLENINPKGAAGARNTCLDYAKGDFIAFLDSDDYWVEDKLEK
QLNFMLENNIQFSYSNYYMFAERFQKKISCRKHNSFNSLTYTCDIGCLTVVLSKKAIG
ELRFPYVPKEDYAFWLMLLEQDLMAYNQGGYNAFYRKQKQSLSGNKLKEIVRQYIVLT
NATNLSKCAIFYRLITYGYNAFIKHYVK"
gene 11092..12498
/gene="gnd"
CDS 11092..12498
/gene="gnd"
/note="O082_10"
/codon_start=1
/transl_table=11
/product="6-phosphogluconate dehydrogenase"
/protein_id="BAQ01270.1"
/translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELSQT
FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
HTYKRIDKDGVFHTEWLD"
gene 12744..13910
/gene="ugd"
CDS 12744..13910
/gene="ugd"
/note="O082_11"
/codon_start=1
/transl_table=11
/product="UDP-glucose 6-dehydrogenase"
/protein_id="BAQ01271.1"
/translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
IVDKEIQQFLQSDKINFNATLDKNEAYRDAGYVIIATPTDYDPKTNYFNTSSVESVIK
DVVAINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQTVPNNLISAI
VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
gene 14000..15025
/gene="wzz"
CDS 14000..15025
/gene="wzz"
/note="O082_12"
/codon_start=1
/transl_table=11
/product="O-antigen chain length determinant protein"
/protein_id="BAQ01272.1"
/translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLLVQLW
RGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGSAAP
KVSEIQSMLIGRFSTTFSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETPEGA
QKQLAQYIQQVDDQVNEELEQDLKDNIALQMKNLQDSLKTQEVVAQEQKDLRIRQIQE
ALQYANQAQVTKPQIQQTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQTRQN
LLDIDNLDVDKLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNA
LRNYNAK"
gene complement(15121..>15306)
/gene="hisI"
CDS complement(15121..>15306)
/gene="hisI"
/codon_start=1
/transl_table=11
/product="phosphoribosyl-AMP cyclohydrolase"
/protein_id="BAQ01273.1"
/translation="TKRIAQKVGEEGVETALAATVHDRFELTNEASDLMYHLLVLLQD
QDLDLTTVIENLRKRHQ"
ORIGIN
1 ggatttcata accagattat cggtgccaac atcaccaatt gtaagttcag cgacttacag
61 ggggacgcta ttgaatggaa cgtggcgatt aacgacagtg atattttgat ctccgaccat
121 gtcatcgagc gcatcaactg tactaacgac aaaatcaact ggggcatcgg cataggtctt
181 tcgggtagta cctacgataa caactacccg gaagaccagg cagtgaaaaa ctttgtcgtg
241 gcgaatatca cgggatcgga ttgtcggcag ttgatccatg ttgaaaatgg caaacatttt
301 gttattcgta atatcaaagc ccgcaatatc acgccggatt tcagtaagaa agcaggtatt
361 gataacgcga cggtcgctat ttacggttgt gacaatttcg tgattgataa tattgaaatg
421 attaatagcg ccgggatgtt aatcggctat ggggtaatta aaggcaaata tctctcgata
481 ccgcaaaatt tccgagtgaa taatattcaa ctggataata cccaccttgc ttacaaattg
541 cgcggcatcc aaatctccgc cgggaatgct gtctcctttg tggcgctgac taacattgag
601 atgaagcgtg cgtcgctgga gttacacaac aaaccgcaac atctttttat gcgtaatatt
661 aatgtgatgc aggaatcctc tgttggaccc gcattgagca tgaacttcga catgcgcaaa
721 gacgttcgcg gcgtctttat ggcgaaaaaa gaaacactcc tgtctcttgc aaatgttcat
781 gcggtgaatg aaagaggaca aagctccgtc gatatcgaca ggattaatca ccatattgtt
841 aatgtggaaa agattaactt tagattgccg gaacggaggg agtagatttg cgaccattcc
901 tggaaaaatg gagccatact taggaacaat gctactgcaa tccacaacga agcggcgtaa
961 catcacaagt aattcagtaa tcaattcagg gtaattgatg ctggcgaaaa aaatcgaaca
1021 agctataatt cagcaaccat tttacaggtg gatgaaataa tgacgaattt aaaagcagtt
1081 attcctgtag cgggtcttgg gatgcatatg ttgcctgcca ctaaggcgat tcccaaagag
1141 atgctaccaa tcgtcgacaa gccaatgatt cagtacattg ttgacgagat tgtggctgca
1201 gggatcaaag aaatcctcct ggtaactcac gcgtccaaga acgcggtcga aaaccacttc
1261 gacacctctt atgaattaga atctctcctt gagcagcgcg tgaagcgtca actactggcg
1321 gaagtacagt ccatttgccc gccgggagtg accattatga acgtgcgtca gggcgaacct
1381 ttaggtttgg gccactccat tttatgtgca cgacccgcca ttggtgacaa cccatttgtc
1441 gtggtgctgc cagacgttgt gatcgatgat gccagcgccg acccgctgcg ctacaacctt
1501 gcggccatga ttgcgcgctt caacgaaacg ggccgcagcc aggtgctggc aaaacgtatg
1561 ccgggtgacc tctctgaata ctccgttatt cagaccaaag aaccgctgga tcgcgaaggt
1621 aaagtcagcc gcattgttga attcatcgaa aaaccggatc agccacaaac gctggattca
1681 gacatcatgg ccgtgggccg ttatgtgctt tctgccgata tttggccgga acttgaacgc
1741 actcagcctg gtgcatgggg gcgtattcag ctgactgatg ccattgccga actggcgaaa
1801 aaacagtccg ttgatgccat gctgatgaca ggtgacagct acgactgcgg taaaaaaatg
1861 ggttatatgc aggcatttgt gaagtatgga ctgcgcaacc tgaaagaagg ggcgaagttc
1921 cgtaaaggga ttgagaagct gttaagcgaa taatgaaaat ctgaccggat gtaacggttg
1981 ataagaaaat tataacggca gtgaagattc gtggcgaaag taatttgctg cgaatattcc
2041 tgccgttgtt ttatataaac aatcagaata acaacgagtt agcaatagga ttttagtcaa
2101 agttttccag gattttcctt gtttccagag cggattggta agacaattag cgtttgaatt
2161 tttcgggttt agcgcgagtg ggtaacactc gtcacatcgt agacatgcat gcagtgctct
2221 ggtagctgta aagccagggg cggtagcgtg tgttaatata gtcaattatt tatcaaaatg
2281 taaataaatg tctctcaaca ttgttcgtac tacactatgt taattttgtc agataagtat
2341 atatttacat tatattaatt tttattttta agtaacagta taattaaatt ttggtgtgaa
2401 gttttttaat tggcatagcg ctatttatat ttcttctaat ccagtctgta attaatgttt
2461 taaggaattt atcgtatgtg tggttttttt atcactaata atccgagtat taattcgtca
2521 catttgaaat tagttgaaga ttcattagca tttcgaggtc ctgatttcag ttctggattt
2581 attaatacaa atggatggat tgtttatcat tcaaggcttt cgatcatcga tttaacagaa
2641 gatgcaaatc agccaatatt cgattctaaa ggtggtgtct tagtatttaa tggggagatt
2701 ttaaatttta aagaattggg tataaaatat tttaataaag acttcgaaag tgattcgaag
2761 ctattgagcg ctttactgga aaggaaactt atcgatttga atgaatttga tggttttttt
2821 tcgtttgttt atgtaaatca caacggcaaa cttgaatatg ctgtccgcga caaatttggt
2881 gttaaacctt tattctatca taaaagcgag gaaggatatt tatcatttag ctccgaacca
2941 agtttgttaa atcgattata taattgtggt ttaaataaag atgcgatcga cgagtattac
3001 tcaattagat accctatttt ttcaggaagt tattttaatg atgtcttttg tattaatccc
3061 ggtgaatgtt atataaatgg tgaatacttt aatattctaa attatttttc aggtaaatat
3121 gttgagacta atatcaatga aatagaatta agtataaaaa aaggaattaa aactcggaaa
3181 gtttcagatg cacctgttgg cttgctttta agtaaaggca tagatagtaa tcttttaaat
3241 actctttcag agtttaattt taagtatagt attggcttta aaggcgatgc agatttagaa
3301 tacttaaagc agcaaaatat aaaaaatcta aaaattgtcg aatgtaataa tcaattatac
3361 aaggatgcat ttgattattt gattaaatta cgtggtgaac ctatgtcagt tcctaacgaa
3421 gttttattat atttaatagc aaaagaagca gcttcggatg gagtaaaagt attgttatca
3481 ggagaagggg ctgatgaatt ttttgggggc tatgatcgta tttttacttg ggctttatat
3541 gcgcaagagt ttgtattaga agattttatt gaacattact gttatgagct gccagcggaa
3601 aatactaaat tagtgtataa atttaaatca atatttgaac aagtatctca tctaacacct
3661 ttcgaaaaag ttcgttggtt ctttatccga taccacatgc caatattgtt tagacgcctt
3721 gatttttcac taatggctgc aggtatagaa gggcgcgaac cattagccaa tattcataca
3781 tttatgcagg cgatcaaagt atcaccatca cagttaatga cagacggatt gggtaaaata
3841 ccgttacgca atattattgc taagtatatg ggaaaagact ttgctttctc aagtaaagtt
3901 ggcttccctg ttgatttacg gaaagttttc gattatacgg gcaatgaaac ttcttatgaa
3961 atatggttta aagaaaattt aaaaggggtt ggaatatgat tattggttat acaacagggg
4021 tatttgatct ttttcatatt ggtcatgtaa atttgctaaa aaatgcaaag tctttatgtg
4081 ataaattaat tgtcggggtg actatagacg aattggtact atataagggt aaaagatctg
4141 taatcccttt tcatgaaaga atacaagttg tccaagcatg taaatatgtt gatgtggctt
4201 taccgcaaag tgatatggac aaactaacag ctgtaaaaaa atatcatgca aatatattat
4261 ttgttggtga tgattggtat aacactgaaa aatggtgcgt ttacgaaaaa gaattggcag
4321 aaattggatg taaaataatc tatttccctt atactcaggg cacttcaagt acattaatta
4381 atgatacatt aaaaaaatta agaaataatc aataaggata aggagcattt attgctcctt
4441 aatacattaa gttattaaat ttgcaagtgc cattatgttt aaacacagtg tatttataaa
4501 tgcaactgca ttgtttttta tgcagcttct cggttatata tcgccacttt tagtttatcc
4561 ctatttaacc agggtgctcg ggatatctgg atttggacag tatgctatgg ccatatcaat
4621 tactgcctta tctttcataa ttaccgattt tggatttgcc ctttcaggtg ctaattggct
4681 ggccaaaaac agaaataaaa ctaaagaagt aaaacactat ctctcaaatg tatgtttatt
4741 aaaatttata ttcttcgctc tttgttctgt tgtatcaagc ttggttataa ttacttcaca
4801 acaactttca tacttgttac attcaatacc tgcaattatt tttttattgt tttcgcaaac
4861 attccaaatg aattggctat tccaaggatt agagaaaatg tctttggcta tatatccttt
4921 tgttatatcg aggattatct ttatattgac ggtgtttata tttgttaaat ataaagcaga
4981 tgtcaatatt gctatttttt tattaggttt ttcaaatttg ctgtcagtta taattagtgc
5041 ttgtctgtta gcgcgagagg gtttgagttt ctgcaagccg agttttaggg attcattcaa
5101 tattttaaag aataatattt ctttctttct ttcaagagca tctgttagta tttatacaag
5161 tgcaagtgca tttattttag gttatactac aaatgttcat gctgtagcat attattctgc
5221 agcagaaaaa ttatatacag ctggtcaatc attaactgga actgtaagcc aggcgttgta
5281 cccatatcta tcgcgaacag gaaataaaaa tatattcaat atagccttga taattgttat
5341 gcttcctttg acaataggaa taggaattgc ttttattttc tcatcagaat taattaattt
5401 gatttatggt acagcctatt ctgaatcaat ccctatctta aagatttttt tgatttgttt
5461 aatcattaat ttcatcggcg tatcatatgg ttatccagct tttgcaatta taaatagaat
5521 agatgttgca aacaagacag tatatatggg ggggctattt caaataagtt cattattata
5581 tctctatgta aatgatataa tctctgcaaa cagtgttgct ataagtgttc tcatgactga
5641 aacgttcgtg atgtgtttga gaattttatt ctattatatc ttttcaaaga atgcacatga
5701 ttaaaaaatt aaagcttaag atttatgcac tttcttcata tttgctagga cattttctaa
5761 atgcagatga cgacttatgg attattggtg caggtaatgg gtatgtattt ggggataatt
5821 cgcgatattt ttatcagtgg gttatcgaga aaaaaaataa aaaaatattt tgggttacaa
5881 aagatctaaa aatatatcat gcactatcca aaaatgagaa aacgaaagat agagtgattt
5941 atttttattc gctgaaaagt atactattta ttgcgcgcgc aaaagttttt atttgcagtc
6001 atggtgtcaa tgatataaca ccatatgttt gtaaaaatag aaaattgata tgtttgtggc
6061 atggtttgcc tataaaaaaa ataggtgatt taacactaag ttcatatgga aaagataatc
6121 ctcttgaacg gggcataaaa catgtgatta attcaaaaaa agagtatgat gtatttctaa
6181 gtccatctct ttattataaa gaaatatttg aaaaatcttt tgaaggtaaa atatctgaat
6241 ttatatatgc acaatatcct cgtgtaacag agctcaaaaa taaatcgaat gatatcgctg
6301 ataatagtga tataaagaca attttatatg caccaacatt tagagatttt gtcgcccctt
6361 cttattatga aataaatgga atagttccat caaagaaact attaagcgaa ttaaattgta
6421 tcttgggata taatagatat aagttaataa taaaactaca tccctatatt tgtatagaaa
6481 attcatacat gcatgaatta attggtttca ataatattat atttgcaaat aagaatgatg
6541 atgttctaga cctgctgatt aatgccagtg tattaatcac agactattct agtatttgtt
6601 ttgatttctt aacgttggga aaagaaatca ttttcctaat gcctgattat gaccaatacg
6661 ttggtcaaac cagaggttta aactttgaat taaaagattt tgctccagga aagattttca
6721 ctaattggaa tgatttaata aagtatatcc ctgtgattaa tcgcaaattt actttggatg
6781 atgattatct aaaagctttg aagatgtcaa acgaatttca cacgagaaaa aattcctata
6841 tttttgattt tatagaaaaa agagcaaagc tataatggtg aaagagttta aaattaaaac
6901 tgattgctat attattatac tatggttttt tattgctgcc tttaagatct tatttaacaa
6961 tatattatat ttccgtgatg ttgaagtaat acaattcata attacaattt tattttggct
7021 gattggcctg gttattatcc ttagaagatt tttgaatcat gctataaaat tagacacatt
7081 attaattgtt tgttctgttt taatatgctt gtcaatattg aatgcgaatc ttttaacgtt
7141 tgtttttgct gtattggtat ggagctccat ttcttatctc ctggatagaa gggcgtttat
7201 ttttttttac tctgccagtt tgtttttttt aattataact atactaataa gtgtggccct
7261 caatgagcaa gtgttttaca tcgatggtcg ttacggaaaa gttcctacgt ttggcttcga
7321 aaattctaac agttttccgc agttattagt aatatattta ttgatatcta cttcacgtct
7381 acggacctct ataatcttgg gtagtattat cttgtttttt gcttatatgg aattaaaaac
7441 aaggtctatg tacatgatta ttattcttta tccattagtg attttattat taaagttttt
7501 caaaggtaaa atcatttgta ttcttcctct ggttttgatc gttttaaatt ttgtgatggt
7561 tcttaattta aacaatgaat acataaaaag tttggatgtg cttttatcat atagattatc
7621 ttattctttg gagctaatta ataatctatc attctctcaa tggtttactg ggacttcatc
7681 ggaacttggt atcccaatgg atatgtcata catcaactta gtttataaat atggaataat
7741 agcaactgca atgattgtcg ttttagcgat gaaagccttg catgttgctt taagtcatag
7801 tgatagtaag gttgtagcaa tcatagtatg tttcttttgt tatgcgtttg tagagaatgt
7861 acttctgaat tattatctaa atcccacatt atattttatc ttttattttt acgcaaaaaa
7921 caaacagcgt gaatggttga ataataatgc tggataacat tttaaaggtg tatgatagaa
7981 atgataccta aaataataca ttatatatgg cttggtgata ttgataatat gccaaaacta
8041 agcaaaaaat gtatagatag ttggaaaaaa catcttttaa attatgaaat tatattttgg
8101 gatgaatgca aaattagaag cacatttaaa gagaatgaaa ttgagttctt taataaaatg
8161 gttaagctaa aaaaatttgc atttgctgct gattatgcaa gatgtattat ccttgataaa
8221 tttggtggag tgtatttaga taccgatata gaaataatac gtgatattac tccgttactt
8281 tcagtcagtg atgtattcct cggattagaa gacgataata aaccaaattg tgctgttatt
8341 ggtgccacta gcaataatat ttttttgaaa aaattaataa agtttataat tcaagctgat
8401 ggtctcacag aaattcctat attagcgtac gaagcattgc taggtatatc tgaaagattt
8461 tgtgatttaa acaggctcac aataattgat ggtatagcag tatatccgga gcagttcttt
8521 tatccttata atccttatcg cagagattca cttagtcaat tgatgtttag cgatattaca
8581 gaagaaacat acgcaataca tcattgggct aaaacttgga aactaactat ttttgagcgt
8641 ataaaaaaaa gattgtttaa gcagaaataa aaataagctg tattattata ttatagttta
8701 aggttattat tataaatatc tattagcaca tggtaaatac agttatgttt tagtcgtatt
8761 aatgttttct attgtatgag ttgatgagta tgtttattag attgctacaa ttgaattaat
8821 cgaacaatat gattggacag aaggagtgat ttaaatgtcg cgggttacaa ttggtatttt
8881 gacatacaaa cgaatggatt atcttatatc attgataaaa gatctaaatg cggtaaagat
8941 taaaatagac ttgattatat taaataacaa tgaaaatata gacgttttta atgatattgc
9001 agatattgtg gattcaaatt ttattaatta taaatatatt tggaaccaaa aaaatcatgg
9061 tgtatcagaa ggccgccgga ttatagttga aaattgtaat acagactatt taatgctatt
9121 agatgatgat atctatgtcg aagatattaa tttaatttgt aggaaaataa ttaattcatt
9181 cgatagtgat aaattcttag gtgctattgc ttttaatatt attgatttca ataccaaaaa
9241 gaataataga tatgaaatac cacataagaa taaaaaagta aacttaaatg ctgattttta
9301 cacttatctt attattggtg ctggcttagc gcttcgaact tcagccgtaa aagatgtggg
9361 gaatttctct aatacattag ggccttatgg tttcgaagaa atagatgttg cattccggtt
9421 aataaatgct ggatacaaaa taaaatattt gcatgattgc ataattgaac ataaaaaatc
9481 accggatgga cgtttttcta atgagatggt caattattac gccttagtta acaggacgat
9541 tttagcaaag aaatatctga ggaaaaaata ttttatttct tgtctagtaa ttagatcatt
9601 atttttcata gtaaaaacta aaaatataag attgctattc aaagcctggg cggaaattat
9661 aaaaactgaa agtcatgcaa gtgataaatt caaacaatgt ttttatgatt attgcaaagg
9721 cgttagtggt tttttatatt attgaagtat gattggaatc ggctttgtta aatgcatctt
9781 taagataata tgatctttag aatatatttt tgtttatatt gtgtgttaat ttaaacatct
9841 atagtttgaa gaaataactt ggccaggcaa atatgggttt ttcttgagta ttaatatgta
9901 aatgtaattt tctaatatgt tttattctta tcggtgatat tcttcgttaa attcaaaatg
9961 tgcattttta taagattatg ggttattgtt aagtgtttga aaacatttta agagggtaag
10021 tctaataaaa ttcataatca cctatttaat aagggtaata aatattctat gccatatgtg
10081 acaatagtaa tgccagctta taattctgaa atgtatatag cacaggcaat agaatctgtt
10141 ataaatcaaa ctcatactaa tttcgaattg ttgatctgtg atgacggttc aacggatggc
10201 acaactagca ttgtttcaaa atatatagtt caggataaca ggattaagtt attggaaaat
10261 attaacccta agggggcggc aggtgctaga aatacctgtt tagattatgc taaaggagac
10321 tttattgctt ttttagatag tgatgattat tgggttgaag ataaattaga aaagcaatta
10381 aattttatgt tagaaaataa catacaattt agttattcaa attattatat gtttgctgaa
10441 cgattccaaa aaaaaatatc atgtagaaag cacaattctt ttaactctct aacgtacacc
10501 tgtgacattg gctgtttgac agttgtttta agtaagaaag ctatcggtga attaagattt
10561 ccatacgtgc ccaaagaaga ttatgcattt tggttaatgt tattagaaca agatttaatg
10621 gcttataatc aaggtggata caatgctttt tatcgaaaac agaaacaaag tttatctgga
10681 aacaaactta aagagatagt tagacaatat attgttttga caaacgctac taacctatct
10741 aagtgtgcta ttttttatag attaattact tatggatata atgcgtttat aaaacactat
10801 gttaaatagt ttttaaataa aataaaccta taacaagtcg tgatatttaa atgattgcac
10861 aaataattga ttaatattta catgattcta ttgtcattat aaaaagtcat ttgtgatatg
10921 gggtatggta atatataata tttagctgta gtacaaaatt tgttgttaat tatttaatca
10981 atatgagaac ctatccaaac tttgttcact gagcaacttc actttctaag ttaatttatt
11041 aatcacagcg aaatctcata atcatgtggt aacccctgac aggagtaaac aatgtcaaag
11101 caacagatcg gcgtcgtcgg tatggcagtg atggggcgca accttgcgct caacatcgaa
11161 agccgtggtt ataccgtctc tattttcaac cgttcccgtg aaaagacgga agaagtgatt
11221 gccgaaaatc cgggcaagaa actggttcct tactatacgg tgaaagagtt tgttgaatct
11281 ctggaaacgc ctcgtcgcat cctgttaatg gtgaaagcag gtgcaggcac ggatgctgct
11341 attgattctc tcaagccata cctcgataaa ggcgacatca tcattgatgg tggtaacacc
11401 ttcttccagg acaccatccg tcgtaaccgt gagctttctg cagaaggctt taacttcatc
11461 ggtaccggtg tctccggcgg tgaagaaggt gcgctgaaag gtccttccat tatgcctggt
11521 gggcagaaag aagcctatga actggttgcg ccgatcctga ccaaaatcgc cgcagtggct
11581 gaagatggcg aaccgtgcgt tacctatatt ggtgccgatg gtgcaggtca ttatgtgaag
11641 atggttcaca acggtattga atacggtgat atgcagctga ttgccgaagc ctattctctg
11701 ctaaaaggtg gcctgaacct taccaacgaa gaactgtcac agacctttac cgaatggaat
11761 aacggtgaac tgagcagcta cctgatcgac atcaccaaag atatcttcac caaaaaagat
11821 gaagacggta actacctggt tgatgtgatt ctggatgaag cagcaaacaa aggtacgggt
11881 aaatggacca gccagagtgc gctggatctc ggtgaaccgc tgtcgctgat taccgagtct
11941 gtgtttgcac gttatatctc ttctctgaaa gatcagcgtg ttgccgcatc taaagttctc
12001 tctggcccgc aagcgcagcc agctggcgac aaggctgagt tcatcgaaaa agttcgtcgt
12061 gcgctgtatc tgggcaaaat cgtttcttac gcccagggct tctctcagct gcgtgctgcg
12121 tctgaagagt acaactggga tctgaactac ggcgaaatcg cgaagatttt ccgtgctggc
12181 tgcatcatcc gtgcgcagtt cctgcagaaa atcaccgatg cttatgccga aaatccacag
12241 atcgctaacc tgctgctggc tccgtacttc aagcaaattg ccgatgacta ccagcaggcg
12301 ctgcgcgatg tcgtcgcata tgcagtacag aacggtatcc cggttccgac cttcgccgct
12361 gcggttgcct attatgacag ctaccgtgcc gctgttctgc ctgcgaacct gatccaggca
12421 cagcgtgact atttcggggc gcatacttat aagcgcattg ataaagacgg tgtgttccat
12481 actgagtggc tggattaatc tgatttaccc agcaatattt tgaggcccgg catatattgc
12541 cgggcttttt tttgccaatt atcttataga ctaaatttca ctgcttaata ttaacttaat
12601 aaatatcagc tattcttata aagaaaatct gaattgtttt tcgttgcgtt gaccatcgaa
12661 gaacgtagcg ttaaaacttt tagctcttat caggatgcta aaaacatcat gattcatagt
12721 taagttaatt ctgagagcat gaaatgaaaa ttaccatttc cggtactggc tatgtaggct
12781 tgtcaaacgg gcttctaatc gcacaaaatc atgaggttgt ggcattagat attttaccgt
12841 cacgcgttgc tatgctgaat gatcggatat ctcctattgt tgataaggaa attcagcagt
12901 ttttgcaatc agataaaata aactttaatg ccacattaga taaaaatgaa gcctaccggg
12961 atgctggtta tgtcatcatc gctactccaa ccgactatga tcctaaaact aattatttca
13021 atacatccag tgtagaatca gtaattaaag acgtagtagc gataaatcct tatgcggtta
13081 tggtcatcaa atcaacggtt cccgttggtt ttaccgcagc gatgcataag aaatatcgta
13141 ctgaaaatat tattttctct ccggaattcc tccgcgaagg taaagccctt tacgataatc
13201 tccatccttc acgtattgtc attggtgagc gttcagaacg cgcagaacgt tttgcggcgt
13261 tattacagga aggggcgatt aagcaaaata tcccgaccct gttcaccgat tcaaccgaag
13321 cagaagcgat taaacttttc gctaatacct atctggcgat gcgcgtagca tactttaatg
13381 aactggatag ttatgcagaa agtttaggtc tgaatactcg ccagattatc gaaggcgttt
13441 gtctcgatcc gcgtattggc aaccattaca acaatccgtc gtttggctat ggtggttatt
13501 gtctgccgaa agataccaag cagttactgg cgaactacca gactgtgccg aataacctga
13561 tttcggcaat tgtcgatgca aaccgcacgc gtaaagattt tattgccgat gccattttgt
13621 cacgcaagcc gcaagtggtg ggtatttatc gtctgattat gaagagcggt tcagataact
13681 tccgcgcgtc ttccattcaa gggattatga agcgtatcaa ggcgaaaggc gttgaagtga
13741 tcatctacga accggtgatg aaagaagact cattcttcaa ctctcgcctg gaacgtgatc
13801 tcgccacctt caaacaacaa gccgatgtca ttatttccaa ccgtatggca gaagagctta
13861 aggatgtggc agacaaagtc tacacccgcg atctctttgg cagcgactaa catcctgtta
13921 tcagggcgat tttcgccctg attctcttat gttccctttg taataattca ttatttttat
13981 catttatcct atagcattca tggcgattat cgctaaacta tggcgactta aaattatttc
14041 gtccgttagg gtaatgatga gagtagaaaa taataatgtt tctgggcaaa accatgaccc
14101 ggaacagatt gatttactgg tgcagttgtg gcgtggcaag atgacaatta tcatctctgt
14161 cattgttgct attgccctgg ctatagggta tctggccgtt gccaaagaaa agtggacttc
14221 cacagctatc gttactcaac ctgatgtggg acaaatcgct ggttataaca atgccataaa
14281 tgtgatttat ggctcggcag cgccgaaagt gtctgagatc cagtcgatgt taattggtcg
14341 tttcagcacg acattttctg cattagcaga aacgctggat aatcaggaag aacctgaaaa
14401 actgaccatt gagcctaccg ttaaaaatca gtcattacct ttagcggttt cttatgtggg
14461 tgaaacacct gaaggtgcgc agaaacagct ggcgcagtat atccagcaag ttgacgacca
14521 ggtaaatgaa gaactggaac aagacctaaa agacaacatc gcgctgcaaa tgaaaaactt
14581 gcaggattct ctgaagacgc aggaagtggt cgctcaagaa cagaaagatc tgcgtatccg
14641 tcagattcag gaagcgttgc agtatgcgaa tcaggcgcag gtgacaaagc cgcagattca
14701 gcagacacag gacgtaaccc aggatactat gttccttttg gggagcgaag cgctggagtc
14761 gatgattaag cacgaggcga ctcgtccgtt ggtgttctca tcaaactact atcagactcg
14821 tcaaaacctg ctggatatcg acaatcttga cgttgataaa cttgatattc atgcttaccg
14881 ctatgtaatg aaaccgacgt tacctattcg tcgcgatagc ccgaaaaagg caattacctt
14941 gattctggcg gtgctgctgg gcggcatggt tggcgcgggg attgtgttgg ggcgtaacgc
15001 tctgcgtaat tacaacgcga agtaatcttt tcggttttaa agaaaatggg cagggtggtg
15061 acaccttgcc cgtttttttg ccggatgcga caacaatatc gcatccgctt accccgcaac
15121 tcactgatgc cgtttacgca ggttctcaat caccgtcgtt aagtccagat cctgatcctg
15181 caacagcact agcaggtgat acatcaagtc agatgcctca ttggtcagtt caaagcggtc
15241 atgtaccgtt gctgccagtg cggtttccac cccttcttcg cccactttct gcgcaatgcg
15301 tttggt
//