<pre>LOCUS       KJ778790               12892 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain E 49 serotype O79:H40 O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ778790
VERSION     KJ778790.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12892)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12892)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Swimley,M.,
            Baranzoni,G.M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E 49"
                     /serotype="O79:H40"
                     /db_xref="taxon:562"
     misc_feature    1..12892
                     /note="O-antigen gene cluster"
     gene            107..1192
                     /gene="rmlB"
     CDS             107..1192
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="AIG62643.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVINVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPHAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAENIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            1192..2091
                     /gene="rmlD"
     CDS             1192..2091
                     /gene="rmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="AIG62644.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQEANATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRMALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            2149..3027
                     /gene="rmlA"
     CDS             2149..3027
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase 1"
                     /protein_id="AIG62645.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDSAVNKESGATIFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKALAEPL
                     KKNAYGHYLLKMIKGY"
     gene            3032..3568
                     /gene="rmlC"
     CDS             3032..3568
                     /gene="rmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="AIG62646.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSSTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFITLSNIAEFIYKTTQFYNPSFERVIAWDDPVLNINWNIK
                     QLPIVSKKDMKGLPFSNI"
     gene            3583..4344
                     /gene="wbdT"
     CDS             3583..4344
                     /gene="wbdT"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="AIG62647.1"
                     /translation="MENKVSLLVNCLNEVNNLKNNIIPLAHHFDEVVIVDMQSNDGSA
                     ELIKKLDNSRFIEIEKMGYVEPAREIGINSCKYDYVFILDADEILSEKIILEINRFRK
                     GEKTEFIGLVDIKGLMIPRYNSMFSQDITWGKFAPENDKQLRFFKKGTVEISSKIHSG
                     IKFKCNMEVVTLPFSSGYYIYHFHSATALEFIGRVVRYAEYENTKQPGKNSFNVTNAI
                     KAFVKEYILSGGYKHGKTGFALAYILSLREFLKGK"
     gene            4348..5439
                     /gene="wzy"
     CDS             4348..5439
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AIG62648.1"
                     /translation="MRVFLYVLILYLSVWITNEYSILSFPIEFNFLYPLPQILLLSFV
                     YAFITRAKKKSHQVDILEWVIPITKLSIVLSLIVLIITLPANGRDAKLEIISNNIVLY
                     YLNMFNSSMLIVMLSSMFQPCRSIVNKKVFFYIIFAIIAFSLTNLSRSMAFYFIIAIC
                     MSGLKFDIEKKKIISWGMVLIVVMILMPIFQGRTDNVEGALARSILNIVFYVSYSFGL
                     GEYLIKETHLSGISWGYFGYVLSKILNEPLTFNIFFDNKYLYEFIQLGKSDIYGLLNA
                     NVMYPLWATVYADFGLFSFIPYLFTFFIIVTSYFMRAYILFSWLFFRFAILGFLVSPV
                     LFRDIVFEIVVVVAFEIFIRKNKRICVDE"
     gene            5432..6232
                     /gene="wbdU"
     CDS             5432..6232
                     /gene="wbdU"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="AIG62649.1"
                     /translation="MNNFVVIYVCYGDNFNYEIINAKIRRFLAPLDIKAIVIANTKEE
                     YIYFYDENGRTIKKYSGLNSEMEFSGYFYGVEEYFERRNIKNMSQSYIFMNDTLFSHG
                     KLRKIERLGLTEWKLRSLLFKIKNKEIHGFWHKSIYLRETELKKGYFNSKFFILHNWN
                     FTEIKSILNLNGVVVTINDASHYENNIFMSDKYSRFLQRWLHESDGWYKAQELNCENK
                     KNFLKKATSIVHEHYITKFIEENRIKKHCLINNSRLAKFVMKFIRLEY"
     gene            6232..7392
                     /gene="wzx"
     CDS             6232..7392
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AIG62650.1"
                     /translation="MRAGAIVFLRNLLIAVSGFILVSKGVKNLSEADTAFLLLVMLVI
                     NAQNALYEGLFLVHTMSFKDKCARTILKRQNKIQVLIPVLAIIFVLIYSNTVLDISIG
                     YGYVALLYFCFYGNILSISVVNVFCSIRGFTWCFILDIVFTTAFIILSVTYSSRIILI
                     LALLLRVGGSAFAGLIFQVRYKKSNDKKLLSLPLYSMGYFSGTLLSLFRDTLSPLLIG
                     WFVGPSILIAIRIFNTCYSAPGLFASALNKIIVRYARKKQSAKKVFITYLIGLYVLSI
                     GYLLIWYIGGERLYLLLFGKTQYFPQTIFLYSISLFCLFWPLGQTAITGMIFTGSSRL
                     FFIVSIIWTFVSAICIFILFIYGIEFYMLAIAFSQMLNILIVYLVRKNETII"
     gene            7376..7978
                     /gene="wbdV"
     CDS             7376..7978
                     /gene="wbdV"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AIG62651.1"
                     /translation="MKLLFNLLYRNLKRCIFRLLIRHYFPCDLSIISSNCIGTRLYQS
                     ADKKYLSPTINLWMPPADFLKFSCNLEFYIQQELQINNNKQFQYPCGRLGDITLHFQH
                     YKNFEVAKKKWDERKDRINWDKILFIFTDRDGATIDSLVKMADVSKNVFVFCSSKKRN
                     SIKRHENIIFIRSKEISIGDLYTNYDELLFKFPFIRFSKI"
     gene            7975..9057
                     /gene="wbdW"
     CDS             7975..9057
                     /gene="wbdW"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="AIG62652.1"
                     /translation="MNKKYFVTDLYAHASEHSLFSKAFIQYFKDKDTIFLLNSIHQNF
                     VDDKSISRTFPFKDSNVKNKWLRLFNREIIKLFTLIAYIPYIVITRRKLCILGASNLQ
                     FFILSFLFFLKPKIIVHGQAESLMTNKNDNSLAARLFINGFQRLAKHKAKLLFLSYHI
                     KNNIKDKGSFYFIKHPLPKNGILGRELSDKKDSKLRVAMVGLLRNDKKNSNLIYSLNV
                     NDNVELWAIGRAHHDFIINHHSNIKFKIWDSVYTDEEFEEAIKDIDAFLYLFNNEQYK
                     MTASATALDAVIYQKIVFTLENDAVQSLLENYKNVYVANSIDELSNLINEFKWENKQA
                     ISKEIINDYILCDNNKDIVTIEEWLK"
     gene            9054..9815
                     /gene="wbdX"
     CDS             9054..9815
                     /gene="wbdX"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="AIG62653.1"
                     /translation="MNEKPLVSIVTPTFNSMKHIEEAYHSLPSQTLDNWEWLITDDCS
                     TDSTYEYLLDLSKHDTRVKPEKLSKNSGAAVARNYSLDRVSGQYIAFLDSDDIWEREK
                     LRMQVIYMEQNNINFSFTMYKCQDENGVLNGKIIDSSHSQSSFSYQDMLKKKATLGCS
                     TVMLRSCLIDQHRMPLIRTGQDYAFWLMLLKENDAFLLKECLTRYRVTPGSLSRNKFK
                     KARRQWQIYRHYEKINVFRAFYFFCHYGFHAIFRK"
     gene            9827..11227
                     /gene="manC"
     CDS             9827..11227
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="AIG62654.1"
                     /translation="MIKPIILAGGNGTRLWPLSRSQFPKQFLPLLDNKYSLFQQTLQR
                     INCLQELSPLIICNETHRFLVSQQLKDIDCNDATALLEPIGKNTAPAIALAAFQCLKD
                     SEDPLLLVLAADHYISDTGIFLESIKKAKKVAEQGKLITFGIIPDYPHTGYGYIERAK
                     EVYDTVYEIRSFKEKPPYDRACEYIETGDYYWNSGMFMFRASVFLNELKKYEPSIYEN
                     CKHAIDSATVDSTFIRPEFHSFNKNPNISIDYAVMERTSIGMVISLDAGWSDVGSWSA
                     LCDINSKYHSSDNNNNNNVISIDSSNNFILSEDNLVATVGIKDLIIINTKDALLVADK
                     NNDQNVKLVVEQIKKLGRNEHVEHPVNYRKWGRFEQIDEGVGFQVNRITIDVGKYISK
                     QYHKYRAEHWIIVSGIAEITLDKVTKICKENESVYVPTNCIHSIKNLGNTILEIIEVQ
                     SGTYFGEDDIIRLSTE"
     gene            11237..12658
                     /gene="manB"
     CDS             11237..12658
                     /gene="manB"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="AIG62655.1"
                     /translation="MRSSSVLISSNVSFGTSGARGLVSDFTHDVCAAFTHAFLSVIQN
                     NFNFRIVALGIDNRPSSYEIAQACAYAIKQHGFEVEYHGVIPTPALAHYSMQKNIPCI
                     MVTGSHIPFDRNGLKFYRPDGEITKEDELAIINSEYTFSPVDVLPHLETSTQGADYYL
                     ERYVSLFNPEILKGKRIGVYEHSSAGRDLYAPLFNQMGAEVISLGRSDEFVPIDTEAV
                     SDEDRILAREWSKKYNLDAIFSTDGDGDRPLVADENGEWLRGDILGLLTAIELNIKAL
                     AIPVSCNTAIEESNKFASIQRTKIGSPYVIAAFADLAKQFDSVAGFEANGGFLLASDL
                     QINGKELKSLPTRDAVLPALMLLIASRNSTISQLINNLPQRFTWSDRVKNFPSDSSQQ
                     IIKNAISSPNNFFNSLGYESLSCSAIDETDGARFTLNNGDIIHLRPSGNAPELRCYAE
                     ASDENQAKQYVTNVLGNITSLIS"
ORIGIN      
        1 attggtagct gtaagccaag ggcggtagcg tgcattaata cctctattaa tcaaactaag
       61 agccgcttat ttcacagcat gctctgaagt aatatggaat aaaagagtga agatacttgt
      121 tactggtggc gcaggattta ttggttctgc tgtagttcgt cacattataa ataatacgca
      181 ggatagtgtt attaatgtcg ataaattaac gtacgccgga aacctggaat cacttgctga
      241 tgtttctgat tctgaacgct atatttttga acatgcggat atttgcgatg ctgctgcaat
      301 ggcacggatt tttgctcagc atcagccgca tgcagtgatg cacttggctg ctgaaagcca
      361 tgttgaccgt tcaattacag gtcctgcggc atttattgaa accaatattg ttggtactta
      421 tgtccttttg gaagccgctc gcaattactg gtctgctctt gatagcgaca agaaaaatag
      481 cttccgtttt catcatattt ctactgacga agtatatggt gatttgcctc atcctgacga
      541 ggtaaataat acagaagaat tacccttatt tactgagaca acagcttacg cgccaagcag
      601 cccttattcc gcatccaaag catccagcga tcatttagtc cgcgcgtgga aacgtaccta
      661 tggtttaccg accattgtga ctaattgctc taacaattat ggtccttatc atttcccgga
      721 aaaattgatt ccattggtta ttctcaatgc tctggaaggt aaagcattac ctatttatgg
      781 taaaggggat caaattcgcg actggctgta tgttgaagat catgcgcgtg cgttatatac
      841 cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacaca acgaaaagaa
      901 aaacatcgat gtagtgctca ctatttgtga tttgttggat gagattgtcc cgaaagagaa
      961 atcttaccgc gagcaaatta cttatgttgc cgatcgtccg ggacacgatc gccgttatgc
     1021 gattgatgct gagaacattg gtcgcgaatt gggatggaaa ccacaggaaa cgtttgagag
     1081 cgggattcgt aaaacggtgg aatggtacct gtccaataca aaatgggttg ataatgtgaa
     1141 aagtggtgcc tatcaatcgt ggattgaaca gaactatgag ggccgccagt aatgaatatc
     1201 ctcctttttg gcaaaacagg gcaggtaggt tgggaactac agcgtgctct ggcacctctg
     1261 ggtaatttga ttgctcttga tgttcactcc actgattact gtggtgattt tagtaatcct
     1321 gaaggtgtag ctgaaaccgt aagaagcatt cggcctgata ttattgtcaa cgcagccgct
     1381 cacaccgcag tagacaaagc agaatcagaa ccggagtttg cacaattact taacgcgacg
     1441 agtgtcgaag cgatcgcgaa agcagccaat gaagtcggcg cctgggttat tcactactct
     1501 actgactacg tatttccggg gaccggtgaa ataccatggc aggaggcgaa tgcaaccgca
     1561 ccgctaaatg tttacggtga aaccaagtta gctggagaaa aagcattaca agagcattgt
     1621 gcgaagcacc taattttccg tacaagctgg gtctatgcag gtaaaggaaa taacttcgcc
     1681 aaaacgatgt tgcgtctggc aaaagagcgt gaagaattag ccgttattaa tgatcagttt
     1741 ggtgcgccaa caggtgctga actgctggct gattgtacgg cacatgcaat tcgtatggca
     1801 ctgaataaac cagaagtcgc aggcttgtat catctggtag ccagtggtac cacaacctgg
     1861 cacgattatg ctgcgctggt ttttgaagag gcgcgcaaag caggcattcc ccttgcactc
     1921 aacaagctca atgcagtacc aacaacagcc tatcctacac cagcgcgtcg cccccataac
     1981 tctcgtctta atacagaaaa atttcagcag aactttgcgc ttgtcttgcc tgactggcag
     2041 gttggtgtga aacgaatgct caacgaatta tttacgacta cagcaattta atagtttttg
     2101 catcttgttc gtgatggtgg agcaagatga attaaaagga atgatgaaat gaaaacgcgt
     2161 aaaggtatta ttttagcggg tggttctggt acacgtcttt atcctgtgac tatggctgtc
     2221 agtaaacagc tattacctat ttatgataag ccgatgatct attacccgct ttctacactg
     2281 atgttagcgg gtattcgcga tattctgatt atcagtacgc cgcaggatac tcctcgtttt
     2341 caacaactgc tgggtgacgg gagccagtgg gggctaaatc ttcagtataa agtgcaaccg
     2401 agtccagatg gacttgcaca ggcatttatc atcggtgaag agtttattgg tggtgatgat
     2461 tgtgctttgg tacttggtga taatatcttc tacggtcacg acctgccgaa gttaatggac
     2521 tcagctgtta acaaagaaag tggtgcaacg atatttgcct atcacgtaaa tgatcctgaa
     2581 cgctatggtg tcgttgagtt tgataaaaac ggtactgcaa tcagcctgga agaaaaaccg
     2641 ctacaaccaa aaagtaatta tgcggtaacc gggctttatt tctatgataa cgacgttgtc
     2701 gaaatggcga aaaaccttaa gccttctgcc cgtggtgaac tggaaattac tgatattaac
     2761 cgtatttata tggaacaggg gcgtttgtct gttgccatga tggggcgtgg atatgcatgg
     2821 ctggacacgg gaacacatca aagcttgatt gaggcaagca acttcattgc tacaattgaa
     2881 gagcgtcaag ggttgaaagt atcctgtccg gaagaaattg cttaccgtaa agggtttatt
     2941 gacgcagagc aggtgaaagc attagctgag ccgctgaaaa aaaatgctta tggtcattat
     3001 ctgctgaaaa tgattaaagg ttattaataa aatgaacgta attaaaacag agattccaga
     3061 cgtattaatt ttcgaaccga aagtttttgg tgatgagcgt ggtttctttt ttgagagctt
     3121 taaccagaag gtttttgagg aagctgtagg ccgcaaagtt gaatttgttc aggataacca
     3181 ttcgaagtct agtaaaggtg ttttacgcgg gctgcattat cagttggaac cttatgcaca
     3241 aggaaaattg gtgcgctgcg ttgtcggtga agtttttgac gtagctgttg atattcgtaa
     3301 atcgtcatca acttttggca aatgggttgg ggtgaattta tctgctgaga ataagcgcca
     3361 gttgtggata cctgaaggat ttgcacatgg attcataacg ttgtcaaata tagccgaatt
     3421 tatttataaa acaacgcaat tttataaccc gtcatttgaa agagtcatcg catgggatga
     3481 tccagttctt aacattaatt ggaatataaa acaattacct attgtttcta aaaaagatat
     3541 gaaagggtta ccattcagta atatatagta cagggtttca ttatggaaaa taaagtatct
     3601 cttctagtta attgtcttaa tgaagttaac aatctgaaaa ataatattat acctctggcg
     3661 catcattttg atgaagtagt aatcgttgat atgcaaagta atgatggttc cgcagaatta
     3721 ataaaaaagt tagataattc gagatttata gaaattgaaa agatgggata tgttgagccg
     3781 gcacgagaaa taggtataaa ttcttgtaaa tatgattatg ttttcatatt ggacgcagac
     3841 gaaattttat cagaaaaaat tattttagaa atcaatagat ttcggaaagg tgaaaaaaca
     3901 gaatttatag gattagtaga tattaaaggc ttaatgattc cacgttataa ttcaatgttt
     3961 tctcaagata ttacttgggg taagtttgct ccagaaaatg ataaacagtt gcgctttttt
     4021 aagaaaggaa ccgtggaaat atccagtaag attcattcag gtataaaatt caagtgtaat
     4081 atggaagttg ttacactacc attttcttca ggatactata tttatcactt tcattcagca
     4141 actgcgttag agtttattgg tagagttgtt cgttatgctg aatatgaaaa tactaagcaa
     4201 cccgggaaga acagttttaa tgttacgaat gcaatcaagg cgtttgtaaa agaatatata
     4261 ctaagcggag ggtataaaca tggcaaaact ggatttgcgt tggcttatat tttatctttg
     4321 cgtgagttct taaaaggtaa ataatatatg agagtttttc tatatgtcct catattatat
     4381 ttatcagtat ggataacaaa tgaatatagt attttgagtt ttccaattga atttaatttt
     4441 ctatatccgc tcccacaaat actccttttg tcatttgtat atgcctttat tacaagagcg
     4501 aaaaaaaaat cacatcaagt agatatactt gaatgggtaa taccaattac taaactaagc
     4561 atagttttat ctttaattgt tttaattatt acattgcctg caaatggtcg tgacgcgaaa
     4621 cttgagataa tatcaaataa tatagtgcta tattatttga atatgtttaa cagtagtatg
     4681 ttaattgtaa tgctgtcaag tatgtttcaa ccatgcagat caatagttaa taagaaagtg
     4741 tttttctata taatatttgc tataattgct ttctcattaa caaatctgtc acgcagtatg
     4801 gctttttatt ttataatcgc tatttgcatg tctggattaa aatttgacat agaaaagaaa
     4861 aaaataattt cgtggggtat ggtattgatt gttgttatga tattaatgcc aatatttcag
     4921 gggcgtacag acaatgttga aggtgctttg gcccgtagca tattaaatat tgtattctat
     4981 gtatcttatt catttggtct tggtgagtac ttaataaaag agactcattt atcaggtatt
     5041 agttgggggt attttggcta tgttttgtct aaaatattga atgaacctct cacatttaat
     5101 atatttttcg ataataaata tctttatgaa tttatccagt tgggcaagtc agatatatat
     5161 ggtttattaa atgccaatgt aatgtacccg ctatgggcaa ctgtatatgc tgactttggt
     5221 ctgtttagct ttattccgta tctttttact ttttttatta ttgtgacctc ttattttatg
     5281 agggcgtata tattgttttc ttggctgttt tttagatttg ccatacttgg ttttttagtt
     5341 tcacctgtat tatttagaga tattgtattt gaaattgtcg tggtggtggc ttttgaaata
     5401 tttataagaa aaaataaaag gatatgtgtc gatgaataat tttgttgtga tatatgtctg
     5461 ctatggcgat aattttaatt atgaaatcat taatgcaaag attcgaaggt ttttagcacc
     5521 attagatatt aaggctatcg taatagctaa tactaaagaa gagtatatat atttttatga
     5581 tgagaatgga agaacaatca aaaaatattc tggactaaat agtgaaatgg aattttcagg
     5641 ctatttttat ggtgttgagg aatattttga gagaagaaat ataaaaaata tgagtcagtc
     5701 ttatattttt atgaacgata cattatttag ccacggaaaa ttgagaaaaa ttgaacgact
     5761 tggccttaca gagtggaaat tacggagttt attatttaag attaagaata aagaaattca
     5821 tggtttttgg cataaatcga tatatctacg tgagactgag ctgaaaaaag ggtattttaa
     5881 ttcaaaattt ttcattcttc ataattggaa ttttactgaa attaaaagca ttttaaactt
     5941 aaatggagta gttgtaacca taaatgatgc ttcacattat gaaaataata tctttatgtc
     6001 cgataaatat tcccgttttc tgcagcgctg gttacatgaa agtgatgggt ggtataaagc
     6061 acaagaatta aattgtgaaa ataaaaaaaa cttcttaaaa aaagcgacat ctattgtaca
     6121 tgaacattat ataacaaaat tcattgagga aaatagaata aaaaagcatt gtctaattaa
     6181 taattctaga cttgcaaaat ttgttatgaa atttattagg ttggaatatt aatgagggca
     6241 ggtgctatag tatttttgcg aaatttgctc attgctgttt ctgggtttat tttagtatca
     6301 aaaggagtaa aaaatttaag cgaagcagat actgcatttt tgctattagt aatgcttgtc
     6361 ataaatgcac aaaatgcatt gtatgaaggg ctgtttttag ttcatacgat gtcgttcaaa
     6421 gataagtgtg cacgtactat tttaaaaaga caaaataaaa tacaagtgct aattcctgtg
     6481 cttgctataa tttttgtatt gatctatagc aatacagttt tggacatctc tataggttat
     6541 ggctatgtag ctcttttgta tttttgtttt tatgggaata ttctctcaat tagtgttgtt
     6601 aatgtatttt gttcaataag gggatttaca tggtgtttta ttcttgacat agtattcaca
     6661 acagcattta ttattctttc tgttacttat tcaagtagaa taatattgat tttggctctg
     6721 ctcctaagag ttggtggaag cgcttttgca ggtttgatat ttcaagtgag gtataaaaaa
     6781 agtaatgata agaaattgtt atcattaccg ctatactcta tgggatactt ttctggcacg
     6841 ttgctttcat tatttagaga taccttatca ccattattga ttggatggtt tgttggccca
     6901 tcaattctga ttgcaattag aatttttaat acgtgctata gtgcaccagg attgtttgcg
     6961 agcgcattaa ataaaattat agtcaggtat gctaggaaaa agcaaagtgc caaaaaagtt
     7021 ttcatcactt atctgatcgg attatatgtt ttatcaatag gttatctatt aatttggtat
     7081 ataggcgggg agcgattgta tctccttttg tttgggaaga cacaatattt cccccaaacg
     7141 atttttcttt attcaatatc tctcttttgt ttgttctggc cattagggca gacggcaatc
     7201 acaggtatga tttttactgg ttcgtctcgt ttatttttta tcgtttctat tatatggaca
     7261 ttcgtatctg caatatgcat atttatatta tttatatacg gaattgaatt ttatatgtta
     7321 gcgattgcat tttctcagat gttaaatatt cttatagtat acttggtgag aaaaaatgaa
     7381 actattattt aatttgttgt atagaaacct aaaaagatgt atttttagat tgttgattag
     7441 acattacttt ccctgtgatt taagtattat atcttccaat tgcattggga ctcggttata
     7501 tcaaagtgca gacaaaaaat acctgtctcc tacaattaat ttatggatgc cacctgctga
     7561 ttttctaaaa ttttcttgta acctagaatt ttatattcaa caggaacttc agattaataa
     7621 caataaacag tttcagtatc catgcggtcg actgggagat ataacgctac attttcagca
     7681 ttataaaaat tttgaagttg ctaaaaaaaa atgggatgaa cggaaagata ggataaattg
     7741 ggataaaata ctttttattt ttacagatag ggacggtgct acaatcgata gtttagtaaa
     7801 aatggctgat gtttctaaaa atgtatttgt tttctgttcg tctaaaaaga gaaatagcat
     7861 caagagacat gaaaatataa tcttcataag gtcaaaagag attagtattg gtgatttata
     7921 taccaactat gatgagctat tatttaaatt tccatttata agattttcta aaatatgaat
     7981 aaaaaatatt ttgtcacaga tttatatgct catgcctctg agcattcttt attctctaaa
     8041 gcatttatac agtattttaa ggataaagat acgatttttc ttctgaattc aatccatcaa
     8101 aattttgtag atgataaatc catttctcgt acttttccat ttaaagatag taatgtaaaa
     8161 aacaaatggc tcaggctttt caatagggaa ataataaagc tttttactct gattgcgtat
     8221 attccctata tagttataac tagaagaaaa ttatgcatat tgggggctag taatttgcag
     8281 ttttttatat tatctttttt atttttttta aaacctaaga ttattgtgca cggacaggct
     8341 gaatctttga tgacgaataa aaatgataat tcacttgctg caagactatt tatcaatggt
     8401 tttcaaagat tggcaaagca taaggcaaaa ttattatttc ttagctatca tataaaaaac
     8461 aacataaagg acaaaggaag tttttatttt attaagcatc cattaccaaa aaatggaata
     8521 ttagggagag agctatcaga taaaaaagat tctaagctta gagttgcaat ggtgggatta
     8581 ttgagaaatg ataaaaaaaa ttccaattta atttattcat taaacgttaa tgataatgta
     8641 gaactatggg cgataggtcg agctcatcac gacttcatta taaatcatca ctcaaatatt
     8701 aagttcaaga tatgggactc tgtatatacg gacgaagaat tcgaagaggc aattaaggat
     8761 attgatgcat ttttatattt atttaataat gaacagtaca aaatgacagc atctgcaact
     8821 gctttagatg ctgtaatata tcaaaaaatt gtttttactc ttgaaaatga tgctgttcaa
     8881 agtttgctag agaattataa aaatgtgtac gtggctaata gcattgatga gctatctaat
     8941 ttaattaatg agtttaaatg ggaaaataaa caagctatca gtaaagaaat tattaatgac
     9001 tacattttat gcgacaataa taaggatata gttactattg aggaatggct taagtgaacg
     9061 aaaaacctct tgtttcgatt gtaacaccaa cctttaatag catgaagcat attgaagagg
     9121 catatcattc tcttccttca caaacgctag ataattggga gtggttaatt acggatgatt
     9181 gttctacgga ttctacctac gaatatcttc tcgatttatc aaagcacgac accagagtta
     9241 aacctgaaaa attatcaaag aatagcggag ccgcagttgc taggaattac tcattggata
     9301 gagtctctgg acaatatatt gcatttcttg attctgatga tatttgggaa agagaaaagt
     9361 taagaatgca agttatctat atggagcaga ataatattaa cttttcattt actatgtata
     9421 aatgccaaga tgaaaatgga gtgctgaatg gaaaaatcat agattcatct cattctcaga
     9481 gttctttttc ataccaagat atgcttaaaa aaaaagctac actgggttgt tctacggtga
     9541 tgttgagaag ctgcttgata gatcagcata ggatgccttt aataagaacg ggtcaggatt
     9601 atgctttttg gttaatgtta cttaaagaaa atgatgcttt tcttttgaaa gaatgtttaa
     9661 cgcgatacag agtaactcca gggtcacttt ctcgaaataa attcaaaaaa gcaagaagac
     9721 aatggcaaat atacaggcat tatgaaaaaa ttaatgtttt tcgcgctttt tattttttct
     9781 gccattatgg ctttcatgct atatttcgta aataggtaat attaatatga ttaaaccaat
     9841 tatccttgcc ggtggtaatg gtacacgcct ttggcctttg tcgcgttcgc aatttccaaa
     9901 acaattttta cctttgcttg ataataaata tagtttattt cagcaaacct tacaaagaat
     9961 aaattgcctt caggagttat caccactaat catatgcaat gaaactcatc gatttttggt
    10021 ttctcagcag ttaaaagata tagattgtaa tgatgcaact gcacttttag agccaatcgg
    10081 gaaaaataca gcgccagcaa ttgctcttgc agcattccag tgcctgaaag acagtgaaga
    10141 tcctttattg ctcgttttag ctgcggatca ttatatttct gatactggga tttttcttga
    10201 gtctattaaa aaagcgaaga aagttgcaga acaaggaaag ttaattacat ttggtattat
    10261 tccagactat ccacacactg gttatggata tatagaaaga gcgaaagagg tatatgatac
    10321 agtttatgaa attcgctcat tcaaagaaaa accaccttat gatcgagctt gtgaatatat
    10381 agagacagga gattattatt ggaatagcgg gatgttcatg tttcgtgcat ctgttttttt
    10441 gaacgaatta aagaaatatg agcctagtat atatgaaaat tgtaagcatg ctattgattc
    10501 tgccactgta gattctacgt ttattcgtcc tgaatttcat tcatttaaca aaaaccccaa
    10561 tatatcaata gattatgctg taatggagag aacgagtatt gggatggtta tttcattaga
    10621 tgctggttgg agcgacgtcg gctcttggtc tgcactttgt gatataaact caaagtatca
    10681 ttcttcagat aataacaaca ataataacgt tatatcaatt gatagctcga ataattttat
    10741 tctttcagag gataatctcg ttgcaacagt tggtattaag gatcttatta ttattaacac
    10801 gaaagatgct cttcttgtgg ccgataaaaa taatgatcaa aacgtaaaat tagttgtgga
    10861 acaaataaaa aagttaggta gaaatgaaca tgttgaacat cctgtcaatt ataggaagtg
    10921 gggaaggttt gaacaaattg atgaaggagt gggatttcaa gtaaatcgta taacaattga
    10981 tgtggggaaa tatatttcaa aacaatatca taaatatcgc gctgaacatt ggataattgt
    11041 ttctggtatt gctgagataa cattagataa ggtaacgaaa atttgtaagg aaaatgaatc
    11101 tgtatatgtt ccgacaaatt gcattcactc cattaaaaac cttggtaata caatattaga
    11161 gattattgaa gtgcaatcag gaacatattt tggtgaagac gatataatta gattaagtac
    11221 agagtgagaa aatatcatgc gttctagtag tgtattaata agctctaatg tttcatttgg
    11281 cactagcggg gcacgcggtt tagtgagcga ttttactcat gatgtttgtg cggcgttcac
    11341 ccacgctttc ctttcagtga tacagaataa ctttaacttc cgtattgttg ccttaggcat
    11401 tgataaccgc ccaagtagtt acgaaattgc tcaggcatgc gcgtatgcta taaaacaaca
    11461 tggattcgag gttgaatatc atggtgtaat tccgactcct gcattagctc attattcgat
    11521 gcagaaaaac attccctgca taatggtcac agggagtcat atcccttttg atcgtaatgg
    11581 cttaaaattc tacagacctg atggtgaaat cactaaagag gatgagctcg caattataaa
    11641 tagtgagtat acattttcgc ctgtagatgt attacctcat cttgaaacaa gcactcaagg
    11701 tgcagactac taccttgagc gttatgtttc tctttttaat cccgagattt taaaggggaa
    11761 aagaataggg gtatatgaac attctagtgc gggacgcgat ttatatgctc ctctttttaa
    11821 tcaaatgggt gcagaggtca tttccctcgg taggagtgat gagttcgttc cgattgatac
    11881 ggaagcagta agcgatgaag atcgtatact tgcaagagag tggtctaaaa aatataatct
    11941 tgatgctatt ttctcaacag atggcgatgg tgatcgtcct ttagttgccg atgaaaatgg
    12001 tgaatggtta agaggcgata ttctgggact acttactgct attgaactta atatcaaggc
    12061 gttggctatt ccagtgagtt gtaatacagc aattgaagag tctaataaat ttgcaagtat
    12121 acaacgaacg aaaataggtt ctccttatgt aattgcagca tttgcagatc ttgctaagca
    12181 atttgattca gtcgctggtt ttgaagctaa tggtggtttt ctccttgcct ccgatttaca
    12241 aattaatggc aaggaattaa aatcgttacc tacacgagat gctgtgttac cagcattaat
    12301 gctcttaata gcttctcgca atagtactat ttctcaactg attaataatc ttcctcagcg
    12361 attcacttgg tcagatagag ttaaaaactt cccttcagat tcaagtcaac aaattataaa
    12421 aaatgccata tcgtcaccca ataatttctt taatagttta ggttatgaat cattatcctg
    12481 ttccgctatt gatgaaacgg atggtgcaag atttacttta aataatggtg atattataca
    12541 tctccgtcct tccggtaatg ctccagaact ccgttgttat gctgaggcca gtgatgaaaa
    12601 tcaggctaag caatatgtta cgaatgtgct gggaaatatt acctctttga tttcttgatg
    12661 ttataggttt atctacgctt atatgtgtgc gtaggtttga ttacacgtag atgccggtat
    12721 acagaattga agaacggtat ttgttgcatt aatgaaattc agcactacac acattcgtgc
    12781 aacttgagat aacatctcaa tcatattcaa gtcgcgcata catcgcggtg aacaccccct
    12841 gacaggagta aacaatgcca agcaacagat cggcgtcgtc ggtatggcag tg
//

</pre>