<pre>LOCUS       KJ778787               12655 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain E 38 serotype O78:H- O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ778787
VERSION     KJ778787.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12655)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12655)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Swimley,M.,
            Baranzoni,G.M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E 38"
                     /serotype="O78:H-"
                     /db_xref="taxon:562"
     misc_feature    1..12655
                     /note="O-antigen gene cluster"
     gene            74..1216
                     /gene="wffD"
     CDS             74..1216
                     /gene="wffD"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="AIG62613.1"
                     /translation="MKKILLISMSLGKGDYGGGIVSNTNFFALKELEHYELFSVGIVK
                     NTNDAPNFINMVLPGNASKFSTAINNILGFAGQLNNKTTKNIKYIIDEFEPDIVYLDS
                     SLLGCIASYCKKKHKTIQIITFFHNIEFDFEIARIMSGLLHFFPSLISTTLAEYAAVR
                     YSDKIIALHKKDSFRLEEKYGRKADYIVPVCIKDTQREKSFKLVNEKKKEGKKLKVGF
                     IGTAFFANVESAKIISQYIAPKVEGIANFYICGNGFEKYKALNSTNVNVSGYIDSLDD
                     FYNEMDVMIFPIFSGAGMKVKIAESLMYNKPILASAFALVGYEKIIDGTNVISCESHE
                     SFVYHIRQFRRDNNTLYNRKSYYKYFSDKACLHYFRNILREIVNNE"
     gene            1237..2346
                     /gene="pimA"
     CDS             1237..2346
                     /gene="pimA"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose-dependent
                     alpha-(1-2)-phosphatidylinositol mannosyltransferase"
                     /protein_id="AIG62614.1"
                     /translation="MNIIQLSKFYPPIFGGIEQVAQDITEGMQNLCNVDVLSVNNSSK
                     TIYCKNIIRASLLFTLLSTPVSISYILIWSRIRNNYDIIHVHLPNPLAIIALLLFPPK
                     APVVVHWHSDIVKQKIALKFFQPLQNLFLNKVEKIIVTSEIYGSSSPQLQRFQDKIIC
                     IPIGIKSERLPKNETLLKHLKEKYKNKKIVFSLGRLVYYKGFENLVNAANFLPEDTII
                     LIGGGGELYDELADSILSNKLEGKVVLLGEIKYEQLSAYYQVCDVFCLPSIHESEAFG
                     VVQIEAMSYGKPVVSTNIKNSGVPWVNENGISGVVVEPNEPHELAKAILTILNNPAGF
                     SLGALERYRKLFTRDKMISSLIGLYQNIKIGNEKE"
     gene            2343..3767
                     /gene="manC"
     CDS             2343..3767
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase 1"
                     /protein_id="AIG62615.1"
                     /translation="MILPVILAGGAGSRLWPLSRLLYPKQFLCLKGELSMLQTTICRL
                     NGLGCENPVVICNEQHRFIVAEQLYQLNKLTENIILEPAGRNTAPAIALAALATQWHK
                     PDSDPLLLVLAADHVIADEDAFRAAVSNAMPYAEAGKLVTFGIVPRHPETGYGYIRRG
                     AVSAGEQEGVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELKKYRP
                     DILDACKKAMSAVEPDLNFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAGWSDV
                     GSWASLWESCTHTAEGNVCRGDVINYKSENSYVYAESGLVTTVGVKDLVVVQTKDAVL
                     IADRSSVQDVKHVVEQLKADGRHEYRMHREVYRPWGQYDSIDAGARYQVKRITVKPGE
                     GLSVQMHHHRAEHWVVVAGTAKVTINGDIKLLGENESIYIPLGATHCLENPGKIPLEI
                     IEVRSGSYLEEDDIVKFYDRYGRD"
     gene            3837..5225
                     /gene="algC"
     CDS             3837..5225
                     /gene="algC"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="AIG62616.1"
                     /translation="MTRLSCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKRALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAVFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFANWRFNLRSSNTEPVVRLNVESRGDLGLVEKQTTYI
                     INLLTTPPNDEK"
     gene            5212..6171
                     /gene="wavE"
     CDS             5212..6171
                     /gene="wavE"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide synthesis"
                     /protein_id="AIG62617.1"
                     /translation="MMKSENITFVVQGPVRNETKDTLESIRLNFKEAKIILSTWEGSN
                     VKGLSFDDIVFSNDPGPLNIKRGTKIVAQENTNRQIISTYQGLIKVRTPYAVKLRTDT
                     PLRNDNIIKNYIKAQAYGRDYNFSYLEERILVSSINTIDPKSYIQFPYHISDWIYFGK
                     TNDLLKIWDGELIDDNDYFTEQDIKEEISCKRGFEFGRYTAEQLVLYSFLKKNKISEY
                     KHYCDTNNDRVEKVLKVILSNFYCVSPKQMGLCFDKYSDLITPRMNYRSLRSFLGFYF
                     VTINERVWRNYYSQFYKLDTTISEKIEICARRIFIKIMKKRKK"
     gene            6168..7289
                     /gene="wejQ"
     CDS             6168..7289
                     /gene="wejQ"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferases group 1"
                     /protein_id="AIG62618.1"
                     /translation="MSKSMLYIYTHNGYNDAFLKKDVGMVPSVMLNGGKVDSLIYCKE
                     KKGKAVFNNLEIYFTGCGWFISNFKAILFLCRNAIFNRHLDLLIFHLSLKNIVPVLTY
                     KLLFKGELICKMDLNTESAIEYSDKSHKINGIKKKLIRMIISRTDQFYVETIRNYNII
                     NKGIFDVPISGKLKLMPNGVDAKLVREVIKTEGPDVFKENTITIISRHACEAKAPNRI
                     FDVIDVMSELKLNNWTLQILGYFPTIFTDALRIRALECGVNIICDGYNLELYDVYKRL
                     FKSKMFICLSLQESYCISLVEAAVFNNHIISTNVGVAEDLSLKYGNIKIIENYSKNLL
                     SKTIEKTIKNDIKIKKASISDRMLDSYSWDNIITGVMSE"
     gene            7291..8412
                     /gene="wzy"
     CDS             7291..8412
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AIG62619.1"
                     /translation="MGVYFILITVALLISIIVLFHNDVKFTNVYIYTYILLLSLLAGL
                     RFHTGWDWEAYDYFFYELTNSNLFEISKANIFNYEPGFVLLSYISVLLHIPPFLFFSL
                     ITVTLIIKSAYKYLGNFVYIFLLIYLYYGYFHNFSIVRQGVAAALFAYSIRYLIAKSY
                     KYYILIFVAALFHMSAIMLLIVPFLYKIANRIPFSALLLFSLLMVYTSVSELIGMKSL
                     LSGINALNIYINNSSLSYKVGFSFKYLELLIILVLFYNKRFSELIASKFSKKQYYVFR
                     CLIVIEVMIYSLFNDFSIIYERLTVYFEFSHAICIAMIISAFKYKRVQLFLLLILLSL
                     IFVRYYQLFNSPVRVDGEMTHYERFENYCSVFNTKDCQR"
     gene            8415..8792
                     /gene="wejR"
     CDS             8415..8792
                     /gene="wejR"
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="AIG62620.1"
                     /translation="MKRIIIDLDNTITIHNDEPYGKKPVNQDVVNKMKMYKELGYTIT
                     IFTARNMNTFSGNIGKINVHTMPVIIDWLNRNNIPYDEIIVGKPWCGTNGFYVDDKAI
                     RPSEFISLSETEIAELLDKEVKK"
     gene            8789..10411
                     /gene="wejS"
     CDS             8789..10411
                     /gene="wejS"
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="AIG62621.1"
                     /translation="MILINSAAYVNDDLRAEFGLLPPCFLPLGNRRLYHHQIESLRKA
                     FPEEKIYLSLPEEYHIGRYDQQYFNENDITILKTSYTLSQGHSLYRLLNDFNLIECDK
                     LIINYGDTFYHTFEATSDDYFYVSNNIGYYNRASVVINGDKLIINKDNPAVDNGELVI
                     SGFFCFSNIGRLCECLKKMNYDFLSSSELYYSSVATKVLKNDHWYDFGHLNSFFTSRT
                     SITTERESNSLQINNFCVIKKSKKKAKMLGEANWFLSLPPALSIFTPRVFDVREDNDY
                     AEYTIEYLYNLPLSDMAVFCELPKSCWVNVFNSCRDFLNRSKEYSNSKKTLDMSELAS
                     YDKLYYDKTLKRLHDFELTTNIQLTKDVIFNGKRFPSIKKIAEISSRFINPTKSDDVI
                     ITHGDFCFSNILYDFRSQRIKLIDPRGINYNNELSIWGDIRYDLAKLCHSVIGCYDLI
                     IAQKFLLEYDYVDNRIEFELYDDNYSDIVNSFLGVFNSFEEFGYTIEEIQCITIHLFL
                     SMLPLHSDSKLRQEAFIANAYRLFSLLNLEYL"
     gene            10408..11142
                     /gene="wejT"
     CDS             10408..11142
                     /gene="wejT"
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="AIG62622.1"
                     /translation="MTTIVIPMAGQSSRFYKAGYDVPKYKLKINNKSVFFHSLRSFLK
                     FKDHFFLIIGIKGILDEKFVQYEMAELGINDFEIVLLEQQTNGQAETVVKGVKNCKRD
                     ISDILIFNIDTFRREINLPDNFSLKDCSGYLETFLGSGENWSNIMPESDVPNQVKMTA
                     EKQNISEYCCTGLYYFGDYKLLNIAYNNWLENEDTTKEVYIAPLYNHLITCGHKIFYT
                     VINRCDVVFCGVPNEYEKIKNGFDWK"
     gene            11139..12554
                     /gene="wzx"
     CDS             11139..12554
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AIG62623.1"
                     /translation="MRQHRKLVSYAVIITFLFIPIQVILSLIFNYYLAKNVALDVLNA
                     WYMFFALISLFSLLEFSYPILAINFFNSYQGNCKGALSFFIKKSLGVVIPLQIIFVVI
                     YSLFVNPLFIYFGMGLVVRSISNIINACVYAKQNIIIDKVYRFLYAIIMPTAFLFLFY
                     HSDKNIDLYDLTLVWFLSSLFCLIYSLSFLLLKHKKSVNRSEKSFHLPFKYNLKLFFT
                     VFPAIFIYTLSIYYLKIFGSESSSSSTIIYGFFLQIFNVYNLIIILVASYLMPTLSKR
                     FHDGVDVIPLTLKLLDISVLISVISTSFVALLGIPVAEYILKGKIDVISYYYIVAIII
                     IFYIETCQVILTSIGTAIGIYDFHRQSIISAVMVLILSCFLIPHYNAEGLMYSIIISQ
                     FFTCFIYNPKRVINKIGLDKVNYIKRLMVHFLFMLTLIFMGLYFQNDDILPRVVILSF
                     ITLIGVCFLYPNVKKIIVDFL"
ORIGIN      
        1 attggtagct gtaagccaag ggcggtagcg tggattttta ttgcgtaaaa acgtcagtaa
       61 acatagaggt ttaatgaaaa aaatactatt aatatccatg agtttgggaa agggcgatta
      121 tggtggtggt atagtatcaa atacaaactt ttttgcttta aaggaattag agcattatga
      181 gttattttca gtaggtattg taaaaaacac caacgatgca ccaaacttta ttaacatggt
      241 tttgcctggc aacgcgagta aattctcaac tgcaattaat aatatattag ggtttgccgg
      301 gcagttaaat aataaaacta cgaaaaacat aaaatacata attgacgagt ttgagcctga
      361 tattgtatac cttgattcaa gccttctggg gtgtatagct tcttattgca agaaaaaaca
      421 taaaactatt caaataataa cgttctttca taatattgag tttgattttg aaattgcacg
      481 tattatgtca gggttattgc atttttttcc ctcgttaata tccacaactc tcgcagaata
      541 tgcggctgta aggtatagtg acaagattat cgcattacat aaaaaagatt cttttagact
      601 agaggaaaaa tatggaagga aggctgatta tatcgtaccc gtttgcatta aagatacaca
      661 aagagaaaaa agttttaaac tcgtcaacga aaagaaaaaa gaaggcaaaa agttaaaagt
      721 tggctttata ggtacagctt tttttgcaaa tgtagagtct gctaagatta tatctcaata
      781 cattgcgcca aaggtggagg ggattgctaa tttttatata tgtggcaatg gatttgaaaa
      841 atataaagca ttgaattcaa ctaatgtaaa tgtttcaggc tatattgatt ctttggatga
      901 tttctataat gaaatggatg tgatgatatt tcctattttt agcggcgcag gaatgaaggt
      961 gaaaatagca gagtcattaa tgtataataa accaattcta gcttctgcat ttgcattagt
     1021 tggttacgaa aaaataatag atggaactaa tgtgattagc tgtgagtctc acgaatcttt
     1081 tgtttatcat ataagacaat ttagaaggga taataacaca ttatataata ggaagtctta
     1141 ttataaatat ttctctgata aagcatgtct gcattatttt agaaatatat tacgagaaat
     1201 tgttaataac gaatagatag atggactaat aacgtcatga atataataca attatcaaaa
     1261 ttctaccctc cgatatttgg tggaatagag caagttgcac aggacataac cgaagggatg
     1321 caaaatttgt gcaatgttga tgttttaagc gtaaataaca gttctaaaac aatatattgt
     1381 aaaaatataa ttcgagcttc attactattt acactattat ctaccccagt atcgattagc
     1441 tatatactaa tctggagtag aatcagaaat aattatgata taattcatgt tcatttgcca
     1501 aatcctcttg ctattattgc actgttgctg ttcccgccca aagctcccgt cgtagtgcat
     1561 tggcatagcg atatagtaaa acagaaaata gcgttaaagt tttttcagcc tttacagaat
     1621 ttatttttga ataaagtaga aaaaattatt gtgacatctg aaatttatgg gagttcttct
     1681 cctcaattac aaagatttca agataaaata atttgtatac cgatcggtat aaaatcagag
     1741 cgattaccta agaatgaaac gctattaaag catctaaaag aaaaatataa aaataaaaag
     1801 atagttttct ctcttgggag gctggtttat tacaaagggt ttgaaaatct agttaatgca
     1861 gctaactttc ttcctgaaga tacaataatc cttattggtg ggggcggtga gttatatgac
     1921 gagttggctg atagcatttt atccaataaa ctagaaggga aagtggtttt gctgggggag
     1981 ataaaatatg aacagctttc tgcttattat caggtttgtg acgtattttg tcttccttcc
     2041 attcatgaat cagaagcttt tggcgtggtt caaattgagg caatgagcta tggaaaacct
     2101 gttgtatcca caaatattaa aaatagtggg gtcccatggg taaatgaaaa tggtatttca
     2161 ggtgttgtag ttgaacctaa tgagcctcat gaactagcca aagctatctt gacaatttta
     2221 aataatccag ctggtttttc cttaggggca ttagagcgat atcgcaaatt attcactaga
     2281 gataaaatga tctcaagttt gattggactt tatcagaata ttaaaatagg aaatgaaaaa
     2341 gaatgatctt acctgttatt ctggccggtg gtgcaggtag tcgcctttgg ccactttccc
     2401 gcttacttta ccccaaacag tttttatgtc tgaaaggcga gctttctatg ctgcaaacaa
     2461 ctatctgccg cctgaatggt ttggggtgcg aaaatccggt ggtgatttgt aatgagcagc
     2521 accgctttat tgttgcggaa cagttgtatc aactgaacaa actcaccgag aacattattc
     2581 ttgaaccggc aggacgtaat actgcacctg ctattgcgct ggcggcgcta gcaacacaat
     2641 ggcataaacc agatagtgat ccgttactgt tggtgctggc agcggatcat gtgattgctg
     2701 atgaagacgc attccgcgcc gctgtgagta atgctatgcc gtatgccgaa gcgggcaagt
     2761 tggtgacttt tggcattgtg ccaaggcatc cagaaacagg ttatgggtat attcgtcgtg
     2821 gtgcagtgtc tgcgggcgag caggagggag tagcctttga agtggcacag tttgtcgaaa
     2881 aaccgaatct ggaaaccgcg caggcctatg tggcaagcgg cgaatactac tggaacagcg
     2941 gtatgttcct gttccgcgcc ggacgctatc tcgaagaact gaaaaagtat cgtccggata
     3001 ttctcgacgc ctgtaaaaaa gcgatgagcg ccgttgagcc agatcttaat tttattcgtg
     3061 tagatgaaga agcgtttctc gcctgtccgg aagagtcggt tgattacgcg gtaatggaac
     3121 gaacagcgga tgctgttgta gtaccgatgg atgcgggctg gagtgatgtg ggctcttggg
     3181 cttcgttatg ggagagctgc actcacacag ctgaaggcaa cgtttgccgc ggtgacgtta
     3241 ttaattataa atccgaaaac agttatgtgt atgccgaatc gggcctggtc accaccgtcg
     3301 gggtgaaaga tttggtggta gtgcagacca aagatgcggt gttaatagcc gatcgcagct
     3361 cggttcaaga tgtgaaacat gtggttgagc agctcaaagc cgacggtcga cacgagtatc
     3421 ggatgcatcg cgaagtatac cgtccgtggg ggcaatatga ctctatcgat gcaggcgctc
     3481 gctaccaggt gaaacgcata actgtgaaac cgggcgaggg cttgtcggta cagatgcatc
     3541 atcaccgcgc agaacactgg gtggtggttg cgggaacggc aaaagtcact attaatggtg
     3601 acatcaaact gcttggtgaa aatgagtcca tctatattcc actgggggcg acacattgtt
     3661 tggaaaatcc aggaaaaata cctttagaaa taattgaggt tcgctcaggt tcttatcttg
     3721 aagaagatga tatagttaag ttttatgatc gttatgggcg agattaattt cattactgaa
     3781 gttgctaatt gtttaacaaa atacttctgt gtattttttc atataagttt gtaaatatga
     3841 ctagattaag ctgctttaaa gcctatgata ttcgcgggaa attaggcgaa gaactgaatg
     3901 aagatatcgc ctggcgcatt ggtcgcgcct atggcgaatt tcttaaaccg aaaaccattg
     3961 ttttaggcgg tgatgtccgc ctcaccagcg aaaccttaaa acgggcgctg gcgaaaggtt
     4021 tacaggatgc gggcgtcgac gtgctggata tcggtatgtc cggcacagaa gagatctatt
     4081 tcgccacgtt ccatctcggc gtggatggcg gcattgaagt taccgccagc cataacccga
     4141 tggattataa cggcatgaag ctggtgcgtg agggggcccg cccgatcagc ggcgataccg
     4201 gactgcgcga tgtccagcgt ctggcagaag ccaacgactt tccccccgtc gatgaaacca
     4261 aacgcggtcg ctatcagcaa atcaacctgc gtgatgcata cgttgatcac ctgttcggtt
     4321 atatcaatgt caaaaacctt actccgctga aacttgtgat caactccgga aacggcgcag
     4381 caggtccggt ggtggacgcc atcgaagccc gctttaaagc cctcggcgca ccggtggaat
     4441 taatcaaagt gcacaacacg ccggacggca atttccccaa cggtattcct aacccgctgc
     4501 tgccggaatg ccgcgacgac acccgcaatg cggtcatcaa acacggcgcg gatatgggca
     4561 ttgcctttga tggcgatttt gaccgctgtt ttctgtttga cgaaaaaggg cagtttatcg
     4621 agggctacta cattgtcggc ctgctggcag aagcgttcct cgaaaaaaat cccggcgcga
     4681 agatcatcca cgatccacgt ctctcctgga acaccgttga tgtggtgact gccgcaggcg
     4741 gcaccccggt aatgtcgaaa accggacacg cctttattaa agaacgtatg cgcaaggaag
     4801 acgccatcta cggtggcgaa atgagcgccc accattactt ccgtgatttc gcttactgcg
     4861 acagcggcat gatcccgtgg ctgctggtcg ccgaactggt gtgcctgaaa ggaaaaacgc
     4921 tgggcgaact ggtgcgcgac cggatggcgg tgttcccggc aagcggtgag atcaacagca
     4981 aactggcgca acccgttgag gcgattaacc gtgtggaaca gcattttagc cgtgaggcgc
     5041 tggcggtgga tcgcaccgat ggcatcagca tgacctttgc caactggcgc tttaacctgc
     5101 gctcctccaa caccgaaccg gtggtgcggt tgaatgtaga atctcgtggt gatttaggac
     5161 ttgttgaaaa gcagacaaca tatatcatta acttattaac aacccccccc aatgatgaaa
     5221 agtgaaaata ttacattcgt agtgcaaggt ccagtcagaa atgaaacaaa agatacttta
     5281 gaaagtatta ggctaaattt taaagaagct aaaataattt tgtccacatg ggaggggagt
     5341 aacgttaaag gtctttcatt tgacgatatt gtttttagta acgatcctgg ccctttaaat
     5401 ataaaaagag gtactaaaat agttgctcag gaaaatacaa atcgtcaaat aatttcaaca
     5461 taccaaggtt taattaaggt tcgaacacca tatgcggtta agttgagaac agatacgcca
     5521 ttgcgaaatg ataatattat aaaaaactat ataaaagctc aagcatatgg gagagattat
     5581 aatttttcat acttagagga aaggattctt gtatcatcta taaatactat agatccaaaa
     5641 agctatattc aattcccata ccatatttct gattggatct actttggtaa aactaacgat
     5701 ttattaaaaa tttgggatgg ggaattaata gatgataatg attattttac tgagcaggat
     5761 ataaaagaag agattagttg caaaagagga tttgagtttg gaagatacac agctgaacag
     5821 cttgttctat actcattttt aaaaaaaaat aaaattagcg aatataaaca ttattgtgat
     5881 acaaataatg acagggttga aaaggtatta aaagtaatcc tttctaattt ctattgcgta
     5941 agtcctaaac aaatgggact ctgttttgat aaatactcag acttaataac ccctagaatg
     6001 aattacagga gtctccgtag tttcttaggg ttttattttg ttacaattaa tgagagagtt
     6061 tggcgaaatt attacagcca attctataaa cttgacacta caatatcaga aaaaatagaa
     6121 atatgtgcac gcaggatatt tataaagatc atgaagaaga ggaaaaaatg agtaaaagca
     6181 tgttatatat ctacacacat aatggttaca atgatgcatt tttaaaaaaa gatgtaggga
     6241 tggtaccatc tgttatgtta aatggtggga aagttgattc gctaatttat tgtaaagaaa
     6301 agaaaggaaa ggctgttttt aataatttag aaatatattt tactggatgt ggatggttta
     6361 ttagtaactt caaggcgata ctgtttcttt gtcgaaatgc aatatttaat agacatttag
     6421 atttattgat atttcatctc agccttaaaa atatcgtgcc tgtgttaaca tataaacttt
     6481 tatttaaagg ggaattaata tgtaagatgg atttaaatac tgaaagtgcc atcgaatatt
     6541 ctgataaaag ccataaaata aatgggatca aaaaaaaact aataagaatg attatttccc
     6601 gcacagatca attttatgtt gagactataa gaaattataa cataataaat aaaggcattt
     6661 ttgatgtgcc gatttcagga aagttaaaat taatgcccaa cggagttgat gctaaattag
     6721 ttagggaggt tattaaaact gaaggtcctg atgtatttaa agaaaataca ataacaataa
     6781 tctctcgcca tgcttgtgaa gcaaaggctc caaacagaat attcgatgta attgacgtaa
     6841 tgtctgaatt gaaacttaat aactggacat tgcaaatatt gggctatttc ccaacaatat
     6901 ttacggatgc tctaagaata agagctcttg aatgtggagt gaatatcata tgtgacggtt
     6961 ataatttaga gctctatgat gtgtataaaa gattatttaa aagtaagatg tttatatgtc
     7021 tttcgctgca agagtcatat tgtatatcac tagttgaagc tgctgtattt aataatcata
     7081 taatatctac aaatgttgga gtagcagaag atctttcatt aaaatacggt aatattaaaa
     7141 ttatcgaaaa ttattcgaaa aatttactat ccaaaactat tgaaaagaca attaaaaatg
     7201 atattaaaat aaaaaaagca agcatttctg atagaatgtt agactcatat tcttgggata
     7261 atatcataac aggggtcatg agcgaataat atgggagtat atttcatttt aataacagtg
     7321 gcactattga tctctattat cgtattattc cacaatgatg ttaaatttac gaatgtatat
     7381 atatatactt atattttact gctctcatta cttgctggtt taagatttca taccggctgg
     7441 gattgggaag cttatgacta ttttttttat gagctgacaa attctaattt atttgaaata
     7501 agcaaagcaa atatttttaa ttatgaacca gggttcgttt tactatctta tatatctgtt
     7561 ttattgcaca taccaccatt tctctttttc tctttaataa ccgttacatt aattataaag
     7621 tcggcttata agtatttagg gaactttgtt tacatttttt tattaatata tttatattac
     7681 ggttattttc ataatttttc tattgtacga caaggtgttg ctgctgcgtt attcgcttat
     7741 tccattcgtt atttgatagc gaaaagctat aaatattata tattaatatt tgtcgctgca
     7801 ttattccata tgtctgctat tatgttatta attgtcccat ttctttataa aatcgctaat
     7861 cgtataccat tttccgccct attgctattt tcattgttaa tggtttatac ctctgtaagt
     7921 gagctcatag gtatgaaatc attattgtca ggaataaatg cactaaatat atatatcaat
     7981 aatagttctt taagctataa agttggtttt tcgtttaaat atttggagtt attaatcatt
     8041 ctagttctat tttataataa gagattttca gaactaattg ctagcaaatt tagtaaaaag
     8101 caatattatg tatttagatg tttgattgta attgaagtga tgatatattc gcttttcaat
     8161 gactttagca ttatttatga aaggctaact gtttacttcg aattttctca tgctatatgt
     8221 atagcaatga ttatatcagc ctttaagtat aaaagagttc agttgttttt actgttaatt
     8281 ttattgtctc ttatttttgt aagatattat caattattca attctcctgt tcgtgtcgat
     8341 ggagaaatga cacattacga acgatttgaa aactattgtt ctgtttttaa cacaaaagat
     8401 tgtcaaaggt aacaatgaag cgaattatta tagacttgga taatactatc acaatccata
     8461 acgatgaacc atacggtaaa aaaccagtaa atcaagatgt tgtaaataaa atgaagatgt
     8521 ataaagagct gggttataca atcacaatat ttaccgcccg taatatgaat actttttcag
     8581 gtaacattgg gaagataaat gttcatacaa tgcctgttat aattgactgg ttaaatcgta
     8641 ataatattcc atatgatgaa attattgtgg gtaaaccatg gtgtgggaca aatggatttt
     8701 atgttgatga taaggcgatt aggccttcag agtttataag cttgtcagaa acggagattg
     8761 ctgaactact ggataaggag gtaaaaaaat gattctaata aactctgctg cttacgttaa
     8821 tgacgaccta agagctgaat tcggtttgct accaccgtgc tttttacccc ttggtaatag
     8881 aagactatat catcatcaga ttgagtcgtt aaggaaagct tttcctgaag aaaaaatata
     8941 tttatctctg ccggaagagt atcacattgg taggtatgat caacaatact ttaatgaaaa
     9001 tgatattaca atactaaaaa cttcttatac tttatcacaa ggacatagtc tgtatcggtt
     9061 gttaaatgat tttaacttaa tcgaatgtga taaactaatt attaattatg gtgatacttt
     9121 ttatcatacc tttgaagcta cgagtgatga ttatttttat gtatctaata acatcggcta
     9181 ctataaccga gcatctgttg taattaatgg tgataagctt attataaata aagataatcc
     9241 agctgttgat aatggtgagt tggttatttc tggtttcttt tgtttcagta atattgggag
     9301 gttatgcgaa tgcttgaaaa agatgaacta tgattttctg tcgtcttctg aattgtatta
     9361 ctcgtcggtt gcaacaaaag ttttaaaaaa tgatcattgg tatgattttg gtcatttaaa
     9421 cagctttttt acatcaagaa cgagtatcac tactgagcgt gagtctaata gtcttcaaat
     9481 aaataatttt tgtgtgataa aaaaaagtaa gaagaaagca aaaatgctag gcgaggcgaa
     9541 ttggtttttg agtttgccgc ctgcattgag tatcttcact ccgagggttt ttgatgttag
     9601 agaagataat gattatgcag agtatacaat agaatatctt tataatcttc ctttgtctga
     9661 tatggcagtg ttttgtgagt tacctaagtc atgttgggtg aatgtgttta actcgtgtcg
     9721 agatttccta aatagatcaa aagaatactc taatagtaaa aaaacgctag atatgtctga
     9781 actggcatca tatgataaat tgtactatga taagacccta aaaaggttac atgattttga
     9841 attgacaaca aacatacaat tgactaaaga tgttatattt aatggtaaaa gattcccatc
     9901 gataaaaaaa atagcagaaa tatcatcacg ctttattaac ccaactaaga gtgatgatgt
     9961 tataattaca catggcgatt tttgctttag taatatatta tacgatttta gaagtcagag
    10021 aattaaatta atcgatccca gaggtattaa ctataacaat gagttaagca tatggggaga
    10081 tataaggtat gacttggcta agttatgtca ctcggtaatt ggatgttatg atcttattat
    10141 cgctcagaaa tttttgctgg aatacgatta tgtagataac agaattgaat ttgaactata
    10201 tgatgataac tacagcgaca tagtgaattc atttttaggc gtgtttaatt cttttgaaga
    10261 atttgggtat actattgaag agatacaatg tataaccata catttatttt tatcaatgtt
    10321 gccgcttcat tctgattcta aacttagaca agaagcattt atagcaaatg cttatagact
    10381 gttcagtttg ttaaatttgg agtatttatg acaacgattg taattcctat ggcaggacaa
    10441 tcatcacgct tttataaagc aggttatgat gttcctaagt ataagttaaa aattaataac
    10501 aaaagcgtat tttttcattc actacgaagt tttctaaaat ttaaggacca tttttttcta
    10561 attataggta ttaaaggcat actagatgaa aagtttgttc aatatgaaat ggccgaatta
    10621 ggtataaatg attttgaaat tgttttgctc gaacagcaaa caaatggtca agcagagacg
    10681 gttgtgaaag gagtaaaaaa ttgcaagaga gatattagtg acattttaat ttttaatata
    10741 gatacttttc gcagagagat taatttgcct gataatttta gtttgaaaga ttgctctggg
    10801 tatttggaaa catttttagg gtcaggtgaa aattggagta atattatgcc tgaatcagat
    10861 gtccctaatc aagtaaaaat gactgcggaa aaacaaaata tatctgaata ttgttgcact
    10921 ggtttatatt attttggtga ttataagttg ttgaatatag cttataataa ctggctagaa
    10981 aatgaagata ctactaaaga ggtgtatatc gctcctctat ataatcattt gattacatgt
    11041 gggcacaaaa ttttttatac cgttataaac agatgtgatg tggtgttctg tggtgtgcct
    11101 aatgaatatg aaaaaataaa gaatgggttt gactggaaat gagacagcac agaaaattag
    11161 tcagttatgc tgtgattatt acttttttgt ttatacctat acaagttatt ctgtcgttaa
    11221 tttttaacta ttatttagct aaaaacgttg ctctggatgt tttaaatgct tggtatatgt
    11281 tttttgcact catttccctt ttttctttgt tggaattttc ctatcccata ttagcaatta
    11341 atttttttaa cagctatcag ggaaattgta aaggagctct gtctttcttc ataaagaaat
    11401 cattaggggt agtaataccc cttcagatca tatttgttgt aatttatagt ttatttgtta
    11461 acccgctttt tatttatttt ggtatgggtt tggtggtacg tagtatatcg aatattatta
    11521 atgcatgtgt atatgcaaaa caaaatatta ttatcgataa agtttatcga tttctgtacg
    11581 ccataattat gccaactgct ttccttttct tgttttatca ttctgataaa aacattgatt
    11641 tgtatgattt aactcttgta tggtttttga gctcactatt ctgcttaata tattctttgt
    11701 catttttact ccttaagcat aagaagtctg tcaacaggag cgaaaaatca ttccatctac
    11761 cattcaagta taaccttaag ctttttttta ctgtttttcc tgcaattttc atctatacat
    11821 tgtctatata ttatttgaaa atatttggtt cagaaagcag ttctagttct actattatat
    11881 atggtttctt tttgcaaata tttaatgttt ataatttgat aattattttg gttgcaagtt
    11941 atttgatgcc tacgctctca aagcgtttcc atgatggtgt tgatgttatc cctctcacac
    12001 ttaaactatt agatatcagt gtgcttatat ctgttatttc tacttctttt gtggcattgt
    12061 taggtattcc tgttgcggag tatattctta aggggaaaat agatgtaata tcctactatt
    12121 atatagtagc aataattatt atattctata ttgaaacatg tcaggttatt ctaacctcaa
    12181 ttggcactgc cattggtatt tacgattttc ataggcaaag tattatatca gcagttatgg
    12241 ttttaattct gtcatgtttt ttaatacctc attacaatgc tgagggtttg atgtatagta
    12301 tcattatatc tcaattcttt acatgcttta tttataaccc taaacgtgtt ataaataaaa
    12361 ttggattaga taaagttaat tatattaaaa gattaatggt gcactttttg tttatgttga
    12421 ctcttatttt tatggggtta tattttcaaa atgatgatat attgccgaga gttgtgattc
    12481 tttcttttat cacattgatt ggtgtttgtt ttctctaccc aaatgtgaaa aaaataattg
    12541 ttgatttctt gtagtttttt aattttattt gcattttaaa tggaaaatct tgttctatct
    12601 tagaaaggag ttaacaatgc tgaagcaaca gatcggcgtc gtcggtatgg cagtg
//

</pre>