<pre>LOCUS       KJ778786               12461 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain E 3b serotype O75:K95:H5 O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ778786
VERSION     KJ778786.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12461)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12461)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Swimley,M.,
            Baranzoni,G.M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E 3b"
                     /serotype="O75:K95:H5"
                     /db_xref="taxon:562"
     misc_feature    1..12461
                     /note="O-antigen gene cluster"
     gene            107..1192
                     /gene="rmlB"
     CDS             107..1192
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="AIG62602.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNNEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIEVVLTICDL
                     LDEIVPKEKFYCEQINYVADRPGHYCRFAIDAEKVSRELGWKPPETFESGICKTVEWY
                     LSNTKWVDNVKSGAYPSWIEQKYEGRP"
     gene            1192..2091
                     /gene="rmlD"
     CDS             1192..2091
                     /gene="rmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="AIG62603.1"
                     /translation="MNILLFGKTGQVGWEIKRALAPLGNLIALDVHSPDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQEADATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRMALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            2149..3027
                     /gene="rmlA"
     CDS             2149..3027
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase 1"
                     /protein_id="AIG62604.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLSDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDSGQVRVLAEPL
                     KKNAYGQYLLKMVKGY"
     gene            3032..3583
                     /gene="rmlC"
     CDS             3032..3583
                     /gene="rmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="AIG62605.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSCKGVLRGLHFQLPPFEQAKLVRCIVGEVFDVAVDIRPNSETFGSWVGVT
                     LSSENKRQLWIPEGFAHGFLTLSEIAEFVYKTNNYYSSNHERGVIWNDEEINIAWPSQ
                     SEKILSQKDINLPSFRFVQMFSK"
     CDS             3717..4820
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="AIG62606.1"
                     /translation="MTKIVVVSTAPIFPTNNGYKSSVLGRIDELLNEGNEVVLIEINL
                     EKVTEKKDELIPTRFNNVQRYEVKKISRSFIAELQILFDIRTRYEQLFSSADIRDNIK
                     KIIDLEKPSIIIAESIWALQALPIEISARIHCVIHDVAIDFFKEMFVSHNEVVRKILF
                     FNDYLKLKITEENIIKRLRVEQFIFLTEEDKCWYKTRYNIDECCCSLASNHLYVEKIK
                     RTINFQTPFLLIPGSIEFSQNFYGLNWFIKNIYPRLNREIRIVVTGKASDKKIKMLNC
                     GEEITFTGELDFYTYNQLSSTCLGVIAPITTGTGIKIKILEAVQKGIPVLTTKFASKG
                     ICSDLCFYCEEDTDTNFVNLINSFLETTLRVQE"
     gene            4817..5890
                     /gene="wzy"
     CDS             4817..5890
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AIG62607.1"
                     /translation="MNLLLFSVLAFGLILALAHNNKSGDINAYLMFFLVVLMVLISGL
                     RMNDSDYIEYKKMYNEVPILCDFSLASIRDIHGEVGYLFLSSIFKTLCLPFQVFLFFI
                     AFLSLLLTYFSFRKISLIPILSLVFYLSHAFIVRDLIQIRAGLAVSISLYSIIKFKGN
                     KSIITGVLFASLIHSGALIIALCYPFFKKKYITLKMMLFLFLVSVIFSYLNGLNLSIQ
                     LLSQYSLLPTAISNYVGWEEYDYRVSIFTNPVFIKGVFLIVLMHKYVLSDIKNEKIIV
                     LYNLYVLGVLAMVALSGMAILSGRLSSFLTLGESILIVYALFYKRNTSLAFLIFSFLT
                     IVQLGYDLFISNVHPELTLIIFG"
     gene            5897..6985
                     /gene="epsD"
     CDS             5897..6985
                     /gene="epsD"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="AIG62608.1"
                     /translation="MKNNKIGILISKIQNLGPVNVVRGLIKENKKYAFTVFCLTNSVD
                     KNIYDELCCLGAKIVLIPDGTWFSKILFVRNFLKEHPHNILHSHGITADMFSYFLNGV
                     KISTIHNRLDEDYIPLFGAVKGNAIYYLHRFILRRFNHIVACSAAVQSKLKQSKVKTK
                     ITTIQNGIDITRFKTLDSDKKKLLREKHGFDSEKRIFIYCGSLSLRKNIAYLLEHLAI
                     EKNDIFLILGDGELFRYCKDKYSKDLRYIFMGKVECPLEYYQLSDIFVSASLSEGLPL
                     ALLEAASTGCYLYVSDIEPHREIASLLGEENISMFKIKDGSYNYLQPKIKKADYNTLS
                     DDKLYNISDKKMSNLYDKLFVSLLEQRH"
     gene            6990..7763
                     /gene="wbbL"
     CDS             6990..7763
                     /gene="wbbL"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="AIG62609.1"
                     /translation="MIYVSVVSHGHFKILKELGAVTKLNNHSRIKVIIKDNLGESELL
                     DFCQENKITYLRSKEKKGFGENNNEVFSSISSLITKEDFFVVMNPDIYIECSDLLDVV
                     DECGSANVNLATINLYRDFDKKTYDNSVRKFPSAINFFMSFLFKKNDCVVNKNKITKP
                     TYVDWAAGSFLIFNAFFYSKLNGFNEKYFMYCEDIDICWRAKKHFNTSVLYYPCYAAI
                     HLAQFNNRRIFSRHFIWHIKSIILFLLYKNGMLRSSKLL"
     gene            7790..9214
                     /gene="manC"
     CDS             7790..9214
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase 1"
                     /protein_id="AIG62610.1"
                     /translation="MIPVILAGGSGSRLWPLSREKFPKQFLKLTGSLTMLQSTLSRLN
                     NLNADDSIVICNEEHRFIVAEQLRELGKLSNNIILEPKGRNTAPAITLAALAAKRKFA
                     DEDPLILILAADHNIQDEHVFCEAINKASSLASYGKLVTFGIVPFKPETGYGYIRRGD
                     EVPADEQHAVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELKKYRP
                     DILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAGWSDV
                     GSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTKDAVL
                     IADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITVKPGE
                     GLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLSENESIYIPLGAMHCLENPGKIDLEL
                     IEVRSGAYLEEDDVIRCYDRYGRK"
     gene            9297..10667
                     /gene="manB"
     CDS             9297..10667
                     /gene="manB"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="AIG62611.1"
                     /translation="MSSLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKVLGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAHPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVQVMVIHTQEI
                     LSILTS"
     gene            10788..12320
                     /gene="wzx"
     CDS             10788..12320
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AIG62612.1"
                     /translation="MRTKKALHNFKVDLLITFLLVLLGFYIRTVFVSKMGSDVTGVML
                     LFTQLTAYLNLAELGIGIAAASVLYKPLSENEYNKITYIISLLSVIYKYIFVFVLILG
                     VVIGICIYYFIDSVKVVNGVFLYWALFVFNTSLTYSYAKYSTLLTANQRYSAVRKIQG
                     GGKVIIIVFQILILCFTQSFILYLLVETLGIFSQYLIFKKIIGNGNQYLSNEVLLIES
                     DKNLIKKELKIRIKNMFFHKIGAVLVLNTDYLLVSKFLTLSYVTIFGSYMMVFQIVTV
                     LMSSFVNAITAGMGNYLINKSNLEIKEITRQFYVIFIAFATFISLNMFFLVNDFIANW
                     IGVNYTLSNTLVALMIVNVFISVVRVPSDILKNASGHFGDIYYPLLEGVLNITISIIL
                     AIIIGLPGIIIGTIVSNLIVIMLAKPLYLYSKLFNLKNPMRVYFEFISRPMLYSLCVF
                     GVSYLLRDEIYSFKVNTWLDFINKLLLISTPSMLVICAIFSTDSDFRLFFRKIIYVVM
                     KK"
ORIGIN      
        1 ctggtagctg taaagccagg ggcggtagcg tgcattaata cctctattaa tcaaactgag
       61 agccgcttat ttaacagcat gctctgaagt aatatggaat aaaagagtga agatacttgt
      121 tactggtggc gcaggattta ttggttctgc tgtagttcgt cacattataa ataatacgca
      181 ggatagtgtt gttaatgtcg ataaattaac gtacgccgga aacctggaat cacttgctga
      241 tgtttctgat tctgaacgct atatttttga acatgcggat atttgcgatg ctgctgcaat
      301 ggcacggatt tttgctcagc atcagccgga tgcagtgatg cacctggcag ctgaaagcca
      361 tgttgaccgt tcaattacag gtcctgcggc atttattgaa accaatattg ttggtactta
      421 tgtccttttg gaagccgctc gcaattactg gtctgctctt gatagcgaca agaaaaatag
      481 cttccgtttt catcatattt ctactgacga agtctatggt gatttgcctc atccagatga
      541 agtaaataat aacgaagaat tacccttatt tactgagacg acagcttacg caccaagcag
      601 cccttattcc gcatcaaaag catccagcga tcatttagtc cgcgcgtgga aacgtaccta
      661 tggtttaccg accattgtga ctaactgttc gaataactac ggtccttatc actttccgga
      721 aaaattgatt ccactagtaa ttcttaatgc tctggaaggt aaggcattac ctatttatgg
      781 caaaggggat caaattcgtg actggctgta tgttgaagat catgcgcgtg cgttatatac
      841 cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacaca acgaaaagaa
      901 aaacatcgaa gtagtgctca ctatttgtga tttgttggat gagattgtac cgaaagagaa
      961 attttattgt gagcaaatta attatgttgc cgaccgcccg ggacattatt gccgttttgc
     1021 gattgatgct gagaaggtta gccgcgaatt gggttggaaa ccaccggaaa cgtttgagag
     1081 cgggatttgt aaaacagtgg aatggtacct gtccaataca aaatgggttg ataatgtgaa
     1141 aagtggtgcc tatccatcgt ggattgaaca gaaatatgag ggccgcccgt aatgaatatc
     1201 ctccttttcg gcaaaacagg gcaggtaggt tgggaaataa agcgtgcttt ggcacctctg
     1261 ggtaatttga ttgctcttga tgttcactcc cctgattact gtggtgattt tagtaatcct
     1321 gaaggtgtag ctgaaaccgt aagaagcatt cggcctgata ttattgtcaa cgcagcagct
     1381 cacaccgcag tagacaaagc agaatcagaa ccggagtttg cacaattact taacgcgacg
     1441 agtgtcgaag cgatcgcgaa agcagccaat gaagtcggcg catgggttat tcactactct
     1501 actgactacg tatttccggg gaccggtgaa ataccatggc aggaggcgga tgcaaccgca
     1561 ccgctaaatg tttacggtga aaccaagtta gctggagaaa aagcattaca ggaacattgc
     1621 gcgaagcatc ttattttccg taccagctgg gtatacgcag gtaaaggaaa taacttcgcc
     1681 aaaacgatgt tgcgtctggc aaaagagcgc gaagaactgg ctgtgattaa tgatcaattt
     1741 ggtgcgccaa caggtgctga gctgctggca gattgtacgg cacatgcaat tcgtatggca
     1801 ctgaataaac cagaagtcgc aggcttgtac catctggtag ccagtggtac cacaacctgg
     1861 cacgattatg ctgcgctggt ttttgaagag gcacgcaaag caggtattcc ccttgcactc
     1921 aacaagctca acgcagtacc aacaacagct tatcctacac cagctcgtcg tccacataac
     1981 tctcgcctta atacagaaaa atttcagcag aactttgcgc ttgtcttgcc tgactggcag
     2041 gttggtgtga aacgaatgct caacgaatta tttacgacta cagcaattta atagtttttg
     2101 catcttgttc gtgatggtgg agcaagatga attaaaagga atgatgaaat gaaaacgcgt
     2161 aaaggtatta ttttagcggg tggttctggt acacgtcttt atcctgtgac tatggctgtc
     2221 agtaaacagc tattacctat ttatgataag ccgatgatct attacccgct ctctacactg
     2281 atgttggcgg gtattcgcga tattctgatt attagtacgc cacaggatac tcctcgtttt
     2341 caacaactgc tgagtgacgg tagccagtgg gggctaaatc ttcagtacaa agtgcaaccg
     2401 actccagatg ggcttgcgca ggcgtttatt atcggtgaag agtttatcgg tggtgatgat
     2461 tgtgctttgg tacttggtga taatatcttc tacggtcacg acctgcctaa gttaatggat
     2521 gccgctgtta acaaagaaag tggtgcaacg gtatttgcct atcacgttaa tgatcctgaa
     2581 cgctacggtg tcgttgagtt tgataaaaac ggtacggcga tcagcctgga agaaaaaccg
     2641 ctacaaccaa aaagtaatta tgcggtaacc gggctttatt tctatgataa cgacgttgtc
     2701 gaaatggcga aaaatcttaa gccttctgcc cgcggtgaac tggaaattac cgatattaac
     2761 cgtatttata tggaacaggg gcgtttatcc gttgccatga tgggacgtgg ttatgcatgg
     2821 ctggacacgg ggacacatca aagtcttatt gaagcaagca acttcattgc aacaattgaa
     2881 gagcgtcagg ggctgaaagt ttcctgcccg gaagaaattg cttaccgtaa agggtttatt
     2941 gattctggac aggttagggt attagccgag ccacttaaga aaaatgctta tggtcaatat
     3001 ctgctgaaaa tggttaaagg ttattaataa aatgaatgta attaaaactg aaattccgga
     3061 tgtattaata tttgaaccaa aagtatttgg ggatgagcgt ggtttcttta tggaaagctt
     3121 taatcagaaa gttttcgaag aggctgtagg gcggaaagtt gaatttgttc aggataatca
     3181 ttctaaatcg tgtaaaggtg tacttagagg tttacacttt cagcttcctc cctttgagca
     3241 ggcaaaatta gtaaggtgta tagttggcga ggtatttgat gttgcagtag acattagacc
     3301 taattctgaa acatttggtt catgggttgg agtaactctt tcgtcagaaa ataaaaggca
     3361 gctatggatt ccagaaggat tcgcccatgg ttttttaact ttaagtgaaa ttgcagagtt
     3421 tgtttataaa actaacaact attattcttc aaatcatgaa aggggagtca tttggaacga
     3481 tgaggaaatt aacattgcct ggccctctca atcagagaag attctgtcac agaaagatat
     3541 taatttaccc tcatttagat ttgttcaaat gtttagcaag tagtgttatc tttacactgc
     3601 acatagtcat cattttttat gctttaagta aattatattg cacatctata acacaaagcg
     3661 caataatatt tcaacctgat gaaggtttgt gttatttatc tttctaggcg ttttttatga
     3721 ctaaaatagt tgtggtttct acagctccaa tattcccgac aaataatggg tacaaaagtt
     3781 ctgtattagg aagaattgat gagttattaa atgagggtaa tgaggtcgtt ttgattgaaa
     3841 taaaccttga aaaggttaca gaaaagaaag atgaattaat accaacaaga tttaataatg
     3901 ttcaaagata tgaagtaaaa aaaatatcta gatcatttat tgccgagtta caaatattat
     3961 ttgatatcag aactcggtat gaacaattat tttcttctgc tgacattaga gataacataa
     4021 aaaaaataat tgatttagaa aaaccttcta ttattattgc tgagtctata tgggcgttgc
     4081 aagccttgcc tattgaaatt agtgcgagaa tacactgtgt cattcatgat gtggcaattg
     4141 atttctttaa agaaatgttt gtatctcata atgaggttgt acgaaaaatt ttgtttttta
     4201 atgattacct caagttgaaa attactgaag aaaatattat caaacgtttg agagttgagc
     4261 aatttatctt tctaactgaa gaagataaat gttggtataa aacaagatac aatattgatg
     4321 agtgttgttg ttccttagcg agcaatcatc tttatgtaga aaagattaag agaactatca
     4381 atttccaaac acctttcctg cttattcccg gtagcattga attttcacaa aatttttatg
     4441 gcttaaattg gtttataaaa aatatatatc ctagattaaa tagggaaata agaatagttg
     4501 taacaggaaa ggcatcagat aaaaaaataa agatgttaaa ctgtggagag gaaattacct
     4561 ttacgggaga gcttgatttt tacacatata atcaacttag ctcaacatgc ttgggtgtca
     4621 ttgcaccgat tacaacgggc actggaatta aaataaaaat attagaagct gtgcaaaaag
     4681 gtattcctgt acttacaaca aaatttgctt caaaaggaat atgttccgat ttatgttttt
     4741 attgcgaaga ggatactgac acaaactttg tcaatttaat taacagtttt cttgaaacta
     4801 cattaagagt ccaagaatga atttactgct tttttcagtc cttgcatttg gtttaatatt
     4861 ggctttggcc cataataata aaagtggaga tattaacgca tacttaatgt tttttctcgt
     4921 ggtcctaatg gtattaatct cagggctgcg tatgaatgat agcgattata tcgaatacaa
     4981 gaaaatgtat aatgaagtac ctattttatg tgactttagt ctcgcatcta taagagatat
     5041 acatggggag gtaggctatc tattcttatc atcaatcttt aaaactttat gcttgccatt
     5101 tcaagtattt ctttttttta ttgctttttt atcactcctg cttacatatt tctcattcag
     5161 aaagataagt ttaataccga tactatcgtt agttttttat ttaagccatg cttttatagt
     5221 tagagatttg attcaaatta gggcaggatt agctgttagc atatcattat attcaataat
     5281 taaatttaaa ggaaataaaa gtataattac aggagtttta tttgcttctt tgattcattc
     5341 tggggcgctt attattgctc tttgctatcc ttttttcaaa aaaaaataca taacattaaa
     5401 aatgatgttg tttttatttt tagtgtcagt tattttttct tatttgaatg ggcttaactt
     5461 atcgatacaa ctcttatctc agtatagttt gcttccaact gcaatttcga attacgttgg
     5521 ttgggaagaa tatgattatc gggtgagtat atttactaat ccggttttta tcaaaggtgt
     5581 ttttttaatt gtcttaatgc acaaatatgt actttcagat attaaaaatg agaaaattat
     5641 agtgctttat aacttatatg ttttaggtgt attagctatg gttgcattga gtgggatggc
     5701 tattctttca ggccgtcttt catcctttct gacactaggt gaaagcattt taattgtata
     5761 tgctctgttc tacaaaagaa atacatcttt ggcgtttcta attttttctt ttttaacaat
     5821 tgtgcaatta ggatatgatc tctttatttc taatgtgcat cctgagctta ctctgattat
     5881 atttgggtga atctaagtga aaaataataa aataggcata cttatctcta aaatacaaaa
     5941 tcttggacct gtgaacgtag tacgaggatt gataaaagaa aataaaaaat atgcttttac
     6001 tgttttttgc ttaacaaata gcgtagataa aaatatatat gatgagttat gctgtttagg
     6061 agccaagatt gtattaatac cagatggtac ttggttcagc aaaattttat ttgtgagaaa
     6121 ttttttaaag gaacatccac ataatatctt acattcacat gggatcacgg ccgatatgtt
     6181 ttcctacttt ctgaatggcg tgaaaatatc tactattcac aatagactag atgaggatta
     6241 tatcccatta tttggcgcgg ttaaagggaa tgctatatat tatcttcatc gttttatatt
     6301 acgaagattt aatcatatcg ttgcttgctc agcagcggtc caatcaaaac tgaaacaatc
     6361 gaaagtaaaa actaaaataa ccaccatcca gaatgggatt gatataacta ggtttaagac
     6421 acttgactct gataaaaaaa aattattgag ggaaaaacac ggatttgata gtgaaaaaag
     6481 aatatttata tattgcggct cgttatcatt aaggaaaaat atcgcttatc tcttggaaca
     6541 cttagccatc gaaaaaaatg atatattttt aattctaggt gatggtgaac tttttagata
     6601 ttgtaaggat aaatattcta aagatttacg gtatatattt atggggaaag ttgaatgccc
     6661 tcttgaatat tatcaactat cagatatttt tgtttccgct tctttgtcgg agggactccc
     6721 cttggcacta ttagaagctg cctctactgg gtgctattta tatgttagcg atatagagcc
     6781 ccatagagaa atagcatctc tattaggaga ggaaaatatt tctatgttta aaattaagga
     6841 tggctcatat aattacttgc aacctaaaat aaaaaaagct gactataaca ctctttctga
     6901 cgataaactt tacaatatat ccgataaaaa aatgtcaaat ctttatgaca aactttttgt
     6961 ttctttatta gagcagaggc actaatataa tgatttatgt ttcggtagtt tctcatggtc
     7021 atttcaaaat ccttaaggag ttaggagcag taacaaaatt aaataatcac agcagaatta
     7081 aagttatcat caaagataat ttaggagaga gcgagctttt ggatttttgt caggaaaaca
     7141 aaataactta tttaaggtct aaagagaaaa aaggatttgg agagaataat aatgaagttt
     7201 tttcctctat atcttcctta attactaagg aagatttttt tgtggttatg aatcctgata
     7261 tatatattga gtgctctgat ctattagatg tcgtcgatga gtgtggttca gcgaatgtta
     7321 atctagcaac gataaattta tacagggatt ttgataaaaa aacatatgat aactcagtaa
     7381 ggaaatttcc ctcggcaatt aattttttta tgtcattttt atttaagaaa aatgactgtg
     7441 tagtaaataa aaacaaaata acgaaaccaa catatgttga ttgggctgca ggttcttttc
     7501 taatatttaa tgccttcttt tattcaaaac tcaacggatt caacgaaaag tactttatgt
     7561 actgcgaaga tattgatata tgttggcgag ctaaaaaaca cttcaatact tcagttttat
     7621 actatccatg ctatgcagca attcatttgg cacaatttaa caatcgtagg atttttagta
     7681 gacatttcat ttggcatata aaaagtatta tccttttttt attatataaa aatggtatgc
     7741 tgcgttctag taagttgctt taatgctaat attcttttaa gaggtgagaa tgatacctgt
     7801 tattttggct ggtggttcgg gaagtcgctt gtggccactt tcacgagaaa agttccccaa
     7861 gcagttttta aagttgactg gcagtttgac aatgttgcag tcaacattgt cacgccttaa
     7921 taatttaaat gctgatgatt caatagttat atgcaacgaa gagcatagat ttattgttgc
     7981 agaacaatta agagagttag gcaaactttc aaataacatt attcttgaac ccaaaggtcg
     8041 taatacagcc cctgctataa cactcgcagc attagcagca aaaagaaaat tcgctgatga
     8101 agatccattg attcttattt tagctgcaga tcacaacatc caagacgaac atgttttctg
     8161 tgaggcaatt aataaggcgt catctttagc tagttatgga aaactagtga cttttggtat
     8221 cgttccattc aaacctgaaa ctgggtatgg ctatattcgt cgcggtgatg aagtgcctgc
     8281 agatgagcag catgcggtgg cctttgaagt ggcgcagttt gtcgaaaaac caaatctgga
     8341 aaccgcgcag gcctatgtgg caagtggcga atattactgg aacagcggca tgttcctgtt
     8401 ccgtgccgga cgctatctcg aagaattgaa aaaatatcgc ccggatatcc tcgatgcctg
     8461 cgaaaaagcg atgagcgccg tcgatccgga tctcgatttt attcgtgtgg atgaagaagc
     8521 gtttctggcc tgcccggaag agtcggtgga ttacgcggtc atggaacgca cggcagatgc
     8581 cgttgtggtg ccgatggatg cgggctggag cgatgtcggt tcctggtctt cattatggga
     8641 gatcagcgcc cacactgccg agggcaacgt ttgccatggc gatgtgatta atcacaaaac
     8701 tgaaaacagc tatgtgtacg ccgaatctgg cctggtcacc accgtcgggg tgaaagattt
     8761 ggtggtagtg cagaccaaag atgcggtgct gattgccgac cgtaacgcgg tgcaggatgt
     8821 gaaaaaagtg gtcgagcaga tcaaagccga tggtcgccat gagcatcggg tacatcgcga
     8881 agtgtatcgt ccgtggggca aatatgactc tatcgacgcg ggcgaccgct accaggtgaa
     8941 acgcatcacc gtgaaacccg gcgagggctt gtcggtacag atgcaccatc accgggcgga
     9001 acactgggtg gtggtcgcgg gaacggcaaa agtcaccatt gatggtgata tcaaactgct
     9061 tagtgaaaac gagtcgattt atattccgct gggggcgatg cactgcctgg aaaacccggg
     9121 gaaaatagat ttagaattaa ttgaagttcg gtctggtgca tatcttgaag aagatgatgt
     9181 tattagatgt tatgatcgct atggacgaaa gtaatatata ataattattt cagaattaga
     9241 aatgattatt ataagttttc atctggataa acaatagata gtatgagttg gaaaatatga
     9301 gttctttaac ttgttttaag gcttacgaca ttcgcgggaa attaggcgaa gaactgaatg
     9361 aagatatcgc ctggcgtatt ggtcgcgcct atggcgaatt tctcaaaccg aaaaccattg
     9421 tgttaggcgg tgatgtccgc ctcaccagcg aaaccttaaa actggcgctg gcgaaaggtt
     9481 tacaggatgc gggcgtcgat gtgctggata ttggcatgtc cggcaccgaa gagatctatt
     9541 tcgccacgtt ccatctcggc gtggatggcg gcatcgaagt taccgccagc cataacccga
     9601 tggattacaa cggcatgaaa ctggtgcgcg aaggggctcg cccgatcagc ggcgataccg
     9661 gcctacgcga tgtccagcgc ctggcagaag ccaacgactt ccctcccgtc gatgaaacca
     9721 aacgcggtcg ctatcagcaa atcaatctgc gtgacgctta cgttgatcac ctgttcggtt
     9781 atatcaacgt caaaaacctc acgccgctca agctggtgat taactccggg aatggcgcag
     9841 cgggtccggt ggtggacgcc atcgaagccc gctttaaagt cctcggcgca cctgtggaat
     9901 taatcaaagt acacaacaca ccggacggca atttccccaa cggtattcct aacccgctgc
     9961 tgcctgaatg ccgcgacgac acccgcaatg cggtcatcaa acacggcgcg gatatgggca
    10021 ttgcctttga tggcgatttt gaccgctgtt ttctgtttga cgaaaaaggg cagtttatcg
    10081 agggctacta cattgtcggc ctgctggcag aagcgttcct cgaaaaaaat cccggcgcga
    10141 agatcatcca cgatccccgt ctctcctgga acaccattga tgtggtgacg gccgcaggcg
    10201 gcacgccggt gatgtcgaaa accggacacg cctttattaa agaacgtatg cgcaaggaag
    10261 acgccatcta cggtggcgaa atgagcgccc accattactt ccgtgatttc gcttactgcg
    10321 acagcggcat gatcccgtgg ctgctggtag ccgaactggt gtgtctgaaa ggaaaaacgc
    10381 tgggcgaact ggtgcgcgac cggatggcgg cgtttccggc aagcggtgag atcaacagca
    10441 aactggcgca ccccgttgag gcgattaacc gcgtggaaca gcattttagc cgtgaggcgc
    10501 tggcggtgga tcgcaccgat ggcatcagca tgacctttgc cgactggcgc tttaatctgc
    10561 gctcctccaa caccgaaccg gtggtgcggt tgaatgtgga atctcgcggt gatgttcagg
    10621 tcatggtaat ccatactcaa gaaatattat caattttgac gtcataaaga ataagccctg
    10681 acaagttagg gcttaattaa tatatatttt tttgaattgg gaatttgtgg aaagattttt
    10741 aatatgttat ttaatgttgt tgaattaatg ttgactggaa aataataatg agaacgaaaa
    10801 aagcattaca caactttaaa gttgatttat taattacttt tttattggtt ttgctagggt
    10861 tttatattcg aactgttttt gtttcaaaaa tgggaagtga tgttactgga gtgatgttac
    10921 tattcacaca gttgacagcg tatctcaatt tggcagaatt aggtattggc attgcagctg
    10981 ccagcgtatt atataaaccg ctcagcgaga atgaatacaa taaaataact tacataatat
    11041 ctttgctctc agttatatac aaatatatat ttgtgtttgt tttgattctt ggcgttgtta
    11101 taggtatctg tatttattac tttattgatt ctgtaaaggt tgtaaacggc gtttttttat
    11161 attgggcttt gttcgttttt aatacatcgt tgacatatag ttatgctaaa tactccacat
    11221 tattaactgc taatcagcgg tactcagcag taagaaaaat tcaaggtggc ggaaaagtta
    11281 taataattgt atttcagata ttaattttgt gctttacgca aagtttcata ctttatttgt
    11341 tagttgagac tttaggtatt ttttctcaat atttaatttt taaaaaaata attgggaacg
    11401 gaaatcaata tctcagtaat gaggttttac ttattgaaag cgataaaaat ttgataaaaa
    11461 aagaattaaa aataagaatc aaaaatatgt tcttccataa aataggcgct gtgctggtcc
    11521 ttaatacaga ctacctgctt gtatcaaagt ttctgacatt aagttatgtg acaatttttg
    11581 gcagctatat gatggtattt cagatagtaa ctgttttgat gtcaagtttt gttaatgcta
    11641 ttactgcagg aatgggtaat tacttaatta acaaaagtaa tttagaaatt aaggaaatta
    11701 cacgtcaatt ttatgtgata tttatcgcct ttgcaacatt catatcacta aatatgtttt
    11761 ttcttgttaa tgattttatc gcaaattgga taggtgttaa ttatacatta agtaacaccc
    11821 tagttgcatt aatgattgtt aacgtattca ttagtgttgt cagggtacct tctgatatat
    11881 taaaaaacgc aagtggacat tttggcgata tttattatcc attattagaa ggtgtgttga
    11941 atattacgat atctatcatt ttggctatca ttattggatt gcctggcatt attataggga
    12001 caattgtatc taacttaata gtaataatgc tcgcgaaacc tttatatctt tactctaagt
    12061 tatttaatct taaaaatccg atgagggttt attttgaatt tatttctcgg cctatgttat
    12121 attcattatg tgtgtttggg gtaagctatt tattgcgcga tgaaatatat tcatttaaag
    12181 taaatacatg gttggatttt attaacaaac tactcttaat ctctactcct agtatgttgg
    12241 taatatgtgc tattttctct acggatagtg actttagatt atttttcaga aaaattatat
    12301 atgtggttat gaagaaataa aaatttcgaa aatgtattaa tcgaaattat gcaacaagct
    12361 ttatttttat aaatgatatg tgatcttttc gcgaatagga gtaaacaatg tcaaagcaac
    12421 agatcggcgt cgtcggtatg gcagtgatgg ggcgcaaccc t
//

</pre>