<pre>LOCUS       KJ778807                9415 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain E 3a serotype O74:K-:H39 O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ778807
VERSION     KJ778807.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9415)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9415)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Swimley,M.,
            Baranzoni,G.M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E 3a"
                     /serotype="O74:K-:H39"
                     /db_xref="taxon:562"
     misc_feature    1..9415
                     /note="O-antigen gene cluster"
     gene            106..1182
                     /gene="rmlB"
     CDS             106..1182
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="AIG62826.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNNEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTINWY
                     LNNNDWCRRVQDGSYQRERLGVIR"
     gene            1179..2042
                     /gene="rmlA"
     CDS             1179..2042
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase 2"
                     /protein_id="AIG62827.1"
                     /translation="MKGIILAGGSGTRLYPITMGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIQDILIITTPEDQQGFIRLLGNGSQFGINLNYATQRNPDGLAQAFIIGEQFIGEDSV
                     CLVLGDNIFFGQGFTPKLRCAAEREIGATIFGYQVMDPERFGVVEFDCNYKALSIVEK
                     PNKPKSNWAVTGLYFYDNNVIDIAKKIKPSHRGELEITSVNEVYLKNNELTVELLGRG
                     FAWLDTGTHDSLIEASNFVETVQRRQGMMVACPEEIAWRNGWLSNEDLYKLGTKIKKN
                     LYGKYLIDLVK"
     gene            2044..2448
                     /gene="fdtA"
     CDS             2044..2448
                     /gene="fdtA"
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-4-oxo-6-deoxy-alpha-D-glucose-3,
                     4-oxoisomerase"
                     /protein_id="AIG62828.1"
                     /translation="MNVEFIPLQKHGDERGMLVALEQAKNIPFEIKRVYYMFGTQGNV
                     RRGYHAHKKIRQVAIPLNGSCRFHLDNGREKIDVVLDDPALGLLIEPGVWHEMYDYSK
                     NCILLVLASDIYDENDYIRNYEDFMRSVKNDS"
     gene            2438..2896
                     /gene="fdtC"
     CDS             2438..2896
                     /gene="fdtC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose
                     3-N-acetyltransferase"
                     /protein_id="AIG62829.1"
                     /translation="MIHKLSDVQTIHIGKKTRVWQYSVILPGAVIGDECNICAHTLIE
                     NDVVIGDRVTIKSGVYIWDGVVIEDDVFIGPCVTFTNDKIPRSKKYPEKFLKTIIKKG
                     ASIGANATILPGITIGENAMVGAGTVVTKDVAPYTVIAGNPGKVIRDIKE"
     gene            2899..4002
                     /gene="fdtB"
     CDS             2899..4002
                     /gene="fdtB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose
                     transaminase"
                     /protein_id="AIG62830.1"
                     /translation="MIDFLNLKRINEKYKAEMQDAFNRVVDSGWYIMGQELKYFESEF
                     AQYCGAKYAIGVANGLDALYLVLKAWKEQGKLSDGDEVLVQANTYIASILAITNNNLV
                     PVFIEPDETTFNLNPAFIRSKITTRTKVILPVHLYGKISPMAEIMDIAEEYKLLVLED
                     CAQAHGAELNKVKAGNWGNAAGFSFYPGKNLGAIGDAGAITTSDDELASILLALRNYG
                     SHVKYENIYQGINSRLDEFQAAVLRVKLPKLDKENERRRQIARKYTAEISNKYMSLPS
                     YTDDLSHVWHLFVVRTQFRKELQLHFSKNEIQTMIHYPIPPHKQLAYTAMSNIHLPVT
                     ERIHNTVMSLPMDPSMTDEHINKVIKVANEFKK"
     gene            3999..5258
                     /gene="wzx"
     CDS             3999..5258
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AIG62831.1"
                     /translation="MRRLLSVTIFTGLLTFMKMCAGFIVSKVVAIYIGPSGLAMLGQI
                     QNIVALFNGLVNSPAGAGVVRFTSQNYDNGDISKCVPWWRASMRWCIIVSSLVIISVI
                     PFNKELSLWLFNDNSYSPVIIITVLILPLTALGTMINSVINGQQKYRVFVSIGMISVI
                     ISTAVMVILIMKFKVFGGLLAVSIQYGVIGFTSLLLCLRFAWVRPNYWFGKTSPDNMK
                     HIFHYMLMAITSAFCLPLSLILIRKILIFYVGWDLTGQWQAVWKISEAYLSVVTLALS
                     TYFLPKLSKLTTYGEIKREITSTVLVIMPLIVIMGIFIYIMRDKIIFLLFTNDFIASI
                     SLFKYQLCGDFIKILAWIFAYPLISQGKVKWYITLEILSAFLFVTLSFIFIQKLGLSG
                     VFFAYIVNYLFYFLFILMNFKRIFKNI"
     gene            5269..6210
                     /gene="pglI"
     CDS             5269..6210
                     /gene="pglI"
                     /codon_start=1
                     /transl_table=11
                     /product="galNAc(5)-diNAcBac-PP-undecaprenol
                     beta-1,3-glucosyltransferase"
                     /protein_id="AIG62832.1"
                     /translation="MHEADIISVIVLAYNSADTITSTLESIAKQEYGAKNIELIISDD
                     ASQDLTISIIENWLVDNYLKFYFVNKIYHHKNLGIVANLDNAYKTASGKWIKVIAGDD
                     ILTSNCLSIYSEFTKKSQGRVFLSYIQSFSQNSIGIVKKDILPPRSQVVLLKHGTLQQ
                     QKKHLMNFSFSATPSLFIEKKLLDDIGYLDKKYRLMEDYPLWHKITDYGERIYFVPEI
                     TVLYRVQESVSRSKEKIVNLDFLTDILRFEKILIKRLSPVSITYHRRKLWCYLYPLLI
                     RLVNNKKNIFSRGCIFLLNVILKPLYLSTIVRRYFEK"
     gene            6226..7047
                     /gene="wbbD"
     CDS             6226..7047
                     /gene="wbbD"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-Gal:alpha-D-GlcNAc-diphosphoundecaprenol
                     beta-1,3-galactosyltransferase"
                     /protein_id="AIG62833.1"
                     /translation="MDNNKHTVAVIMSVYDGDEPYYFEKAIQSIQKQTYQSIDIYLYI
                     DAVQRKDLLNRIRHYKKCSRFHVVTGDKKRGLAYGLNRLLDKIRGKGYGYIARMDSDD
                     ISTTDRIESQVRFLEKNKNIDVIGTNCIEIDGDDNEIFYKKMPETHHELIKSIVKRSP
                     FIHPSVMFRGSVVEHIRYNDSLMNTQDYYLWIDLLSKKYKFHNIQQPLLFFRVNDSFY
                     ARRGFGKLINEVKSRLYAMKKLNKKNVKNYLYILALICLRLAPGNIKKYCYMRFR"
     CDS             7047..7595
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AIG62834.1"
                     /translation="MNCIMFFYGVFFALPLSILNVLPYGMKIDDVILFIALIVFPILS
                     FKKGYHVTIPLSCLLFIIITVLFASLSFLKGINSTNVFLGDSAGTVIGRIIQSLLILI
                     LITFLKNTPHYLLSFFKGYVIAATLSLIIFFGYYVTHINISSFTMRGVYFAKDIFQYN
                     DDLPFTVHVNTLGSFFLIAFFY"
     gene            7673..8221
                     /gene="wzy"
     CDS             7673..8221
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AIG62835.1"
                     /translation="MLAILTYFAYVFYKRNNLNAFLSLIGLLILVLLMPYLYNVYLSL
                     SDYRVYASGRDELYGASLSSIIDNPFGYGLGMQNNILYSITGIDFPAHNILLSIGIEL
                     GLLYLFLLVAYIIGWLLLGKVDYKIIFISFLVIGMFGNAMYFYKFHSLAIALCFLSVW
                     HINFNHKTKQVDLINNENTLHS"
     CDS             8199..9281
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-D-galactose:(glucosyl)lipopolysaccharide-1,
                     6-D-galactosyltransferase"
                     /protein_id="AIG62836.1"
                     /translation="MKILYILNKSVLTGPNIVALTNIEHIHDQGYDVSICFLNNGDDL
                     NNIFPFLKEIEVFYLNLKSLGFISGLKNLNYYINSIKPTIIHSHCFLPDIYNIFNKFF
                     CGPIKNKRVTTIHNIPKEDYRLRYGRIKGSLLLYFHKALFPFFDNCICISKTVQKSIG
                     IKKTSVIYNPVRDVFFNSTKDDVECLTIVYCGHFSQLKNPITLIQLLADSKVNFQFLG
                     IGDGPLLYQCRQLVKKDPRFTFVGRVNHVHKFYEKANCLIHISQTEGFCLSVAEALAS
                     NMYVITNELPVFLELKQALSSEGFYMLNDINNYNLEMVLNSINRKIEAGERCNSGASN
                     RVKALLSPTFIAQQHIVLYQKLTVEK"
ORIGIN      
        1 ctggtagctg taaagccaag ggcggtagcg tgcattaata cctctattaa tcaaactgag
       61 agccgcttat ttcacagcat gctctgaaga atatggaata ataaagtgaa gatacttgtt
      121 actggtggcg caggatttat tggttctgct gtagttcgtc acattataaa taatacgcag
      181 gatagtgttg ttaatgtcga taaattaacg tacgccggaa acctggaatc gcttgctgat
      241 gtttctgatt ctgaacgcta tgtttttgaa catgcggata tttgcgatgc agctgcaatg
      301 gcacggattt ttgctcagca tcagccggat gcagtgatgc acttggctgc tgaaagccat
      361 gttgaccgtt caattacagg tcctgcggca tttattgaaa ccaatattgt tggtacttat
      421 gtccttttgg aagccgctcg caattactgg tctgctcttg atagcgacaa gaaaaatagc
      481 ttccgttttc atcatatttc tactgacgaa gtctatggtg atttgcctca tccagatgaa
      541 gtaaataata acgaagaatt acccttattt actgagacga cagcttacgc accaagcagc
      601 ccttattccg catcaaaagc atccagcgat catttagtcc gggcgtggaa acgcacctat
      661 ggtttaccga ccattgtgac taactgttcg aataactacg gtccttatca ctttccggaa
      721 aaattgattc cactagtaat tcttaatgct ctggaaggta aggcattacc tatttatggc
      781 aaaggggatc aaattcgtga ctggctgtat gttgaagatc atgcgcgtgc gttatatacc
      841 gtcgtaaccg aaggtaaagc gggtgaaact tataacattg gtggacacaa cgaaaagaaa
      901 aacatcgatg tagtgctcac tatttgtgat ttgttggatg agattgtacc gaaagagaaa
      961 tcttaccgcg agcaaattac ttatgttgcc gatcgcccgg gacacgatcg ccgttatgcg
     1021 attgatgcag agaagattag ccgcgaattg ggctggaaac cgcaggaaac gtttgagagc
     1081 gggattcgta aaacaattaa ctggtatttg aataataatg attggtgccg gcgagttcaa
     1141 gatggctctt atcaacgtga gcgtttgggg gtaattagat gaaaggaatt attttagctg
     1201 gtggttctgg aacaaggctt tatccaatta caatgggagt ttcaaagcag ctattaccca
     1261 tttatgataa accgatgatt tattatcctt tgtcagtgct gatgttggca ggtatacaag
     1321 atattcttat tattacaacg ccagaagatc agcaggggtt cataagatta cttgggaacg
     1381 gaagccaatt tggtattaat cttaattacg ccactcaacg gaatcctgat ggtttagctc
     1441 aggccttcat catcggggaa caatttattg gtgaggattc tgtctgctta gttttgggag
     1501 ataatatttt ttttggtcaa ggatttactc cgaaacttag atgtgccgct gagagggaaa
     1561 taggagccac gatatttggt tatcaggtaa tggatcctga acgattcggt gtggtcgaat
     1621 ttgattgcaa ttataaagca ttaagtattg ttgaaaaacc aaataaacca aaatcaaact
     1681 gggctgtaac tggattgtat ttttacgata acaatgttat tgatattgca aaaaaaataa
     1741 aaccgtcgca tcgtggtgaa ttagaaatca catctgttaa tgaggtttac ttaaaaaata
     1801 atgagttaac tgttgaatta ttaggtcgag ggtttgcgtg gctggatacc ggcacacatg
     1861 atagtctcat tgaagcatct aattttgtcg aaaccgttca acgtagacaa ggaatgatgg
     1921 ttgcatgccc cgaagaaata gcttggcgga atggttggtt atctaatgaa gatttatata
     1981 aattaggcac gaaaattaaa aagaatctat acggaaaata tcttatcgat ttggtgaaat
     2041 aatatgaatg tcgagtttat tccattacaa aaacatggtg atgaaagagg gatgttagta
     2101 gccctagagc aagccaaaaa cattccattt gaaattaaac gtgtttatta tatgtttgga
     2161 actcaaggaa atgttcggcg cgggtatcat gcccataaaa aaattaggca agtagcgata
     2221 ccgctaaatg gttcatgccg ttttcattta gataatggtc gtgagaaaat agatgttgtt
     2281 ttagatgatc ctgcattagg gttgcttata gagcctggag tctggcatga aatgtacgat
     2341 tactcaaaga actgtattct tctagtattg gcgagcgata tttatgatga aaatgactat
     2401 attagaaatt atgaggattt tatgaggtct gttaaaaatg attcataaat taagcgatgt
     2461 tcaaactatc catataggta aaaaaacaag ggtctggcaa tattcagtta tattaccggg
     2521 tgctgtaatt ggtgatgagt gtaacatatg tgcacataca cttattgaaa atgatgttgt
     2581 aattggggat agagttacta ttaaatcggg cgtatatatc tgggatgggg ttgttattga
     2641 agatgatgta tttatagggc cctgtgtaac atttaccaat gataaaatcc ccaggtcaaa
     2701 gaaataccca gaaaaatttc ttaagactat tattaaaaaa ggtgcttcca ttggagccaa
     2761 tgcaactata ttacctggaa taacaattgg tgaaaatgct atggttgggg ctggtaccgt
     2821 agtcactaaa gatgttgcac cttatacagt cattgcaggt aatccaggta aagttattag
     2881 ggatattaag gaataaaaat gatcgacttt ctcaacttga aaagaatcaa tgaaaaatat
     2941 aaagcggaaa tgcaagatgc ctttaatcga gttgtcgatt ctggctggta tattatgggg
     3001 caagaattaa aatattttga aagtgaattt gcacagtatt gcggggcaaa atatgctata
     3061 ggtgtagcta atggtttaga tgccctttat ctcgttttaa aagcgtggaa agaacaaggt
     3121 aaattgagtg atggcgatga agtgctggtg caggcaaaca catatattgc ctcaattctt
     3181 gctataacaa ataataatct cgttccggtg ttcattgaac cagatgaaac gacttttaat
     3241 ttaaatccgg catttattcg ttcaaaaata acaactcgga caaaagtgat attgcccgtg
     3301 catctttatg gaaaaatatc accaatggct gaaataatgg acatcgctga agagtacaag
     3361 ctattggtcc tagaagattg tgctcaagct catggtgctg agttgaataa agtcaaagca
     3421 ggcaattggg gaaatgctgc cggatttagt ttttatccgg gtaaaaatct tggggccata
     3481 ggtgatgcgg gggctattac aacatcagat gatgagcttg ccagtatttt gttagcatta
     3541 agaaattatg gttcccatgt taaatatgaa aatatttacc aaggtataaa cagtcgcttg
     3601 gatgaatttc aggctgcagt acttcgagtt aaattaccta aattagataa agaaaatgag
     3661 agaaggcgtc aaatagctag aaagtatact gcagaaatta gcaataaata catgtcatta
     3721 ccttcataca ctgacgattt atcacatgta tggcatttgt ttgttgttag aactcaattt
     3781 agaaaagaat tgcagcttca tttttcaaaa aatgaaatac aaactatgat acattatccg
     3841 atacctccgc ataaacaatt agcatatact gctatgagta atatacattt acccgtaacc
     3901 gaaagaatac ataacactgt tatgtcatta cctatggatc catcaatgac tgatgagcac
     3961 ataaataaag ttattaaagt tgctaatgaa tttaaaaaat gaggagacta ttatcagtaa
     4021 caatatttac aggcctgctg acttttatga aaatgtgtgc aggctttatc gttagcaagg
     4081 ttgttgcaat atatataggc cctagtgggc tggcgatgct tggacagata cagaatattg
     4141 ttgctctttt caatgggctg gttaactccc cggcaggagc tggggttgta cgtttcactt
     4201 ctcaaaatta tgacaatggt gatatttcta aatgtgtgcc atggtggcgc gcaagcatgc
     4261 gctggtgcat aatagtttct tctttagtaa taatttcagt tatcccattt aataaagaat
     4321 tatcattatg gttatttaat gataattctt attcaccagt tatcattata actgttttaa
     4381 tactcccttt aactgcgtta ggaacgatga taaattccgt tattaacggc caacaaaaat
     4441 atagagtttt tgtttctatt ggtatgatat ccgttatcat atcaactgca gtaatggtca
     4501 tacttataat gaagttcaag gtatttggag ggttattagc agtttcaatt caatatggag
     4561 taattggttt tacttcttta ctattatgct taaggtttgc atgggttaga cctaattatt
     4621 ggtttggtaa aacgtcgcca gacaatatga agcatatttt tcattatatg ttgatggcaa
     4681 ttacaagcgc attttgcctt ccactatctc ttattctgat aaggaagata cttatatttt
     4741 atgttggttg ggatttgact ggtcaatggc aagctgtatg gaaaatatct gaggcttacc
     4801 taagcgtggt aactcttgca ttatcaacat attttttacc aaaattatct aaacttacaa
     4861 catatggtga gattaaaaga gaaataacat caacggtttt agtaataatg ccattgatag
     4921 taattatggg aatatttata tatatcatgc gtgataaaat tatctttctt ttattcacga
     4981 atgattttat agcatcaata agcttattca agtatcaatt atgtggtgat tttattaaaa
     5041 tattggcttg gatttttgca tatcctttga tttcacaggg aaaagtaaag tggtatatta
     5101 cattagagat cttatcagcc ttcctttttg ttaccttgag ctttatattt atccaaaagc
     5161 ttggattgtc tggagtattt tttgcataca tagttaatta tttattttat tttctattta
     5221 tactgatgaa ctttaaaaga atatttaaga atatatgagg atattatttt gcatgaggct
     5281 gatataattt ctgttattgt cttggcatac aattctgctg atactataac atctacatta
     5341 gaaagtatag ctaaacaaga gtatggtgca aaaaatattg agttaataat aagtgatgat
     5401 gcttctcagg atttaacaat atctatcata gaaaactggt tagttgataa ctatcttaag
     5461 ttttattttg ttaataagat atatcatcat aaaaatctag ggatagtagc taatttagat
     5521 aatgcatata aaaccgcaag cggtaaatgg ataaaggtaa ttgcaggtga tgacatacta
     5581 acctcgaact gtttatcaat atattctgaa tttacaaaaa aatctcaagg acgagttttt
     5641 ttatcgtata tacagtcatt ttctcaaaac agcattggaa tagttaagaa ggatattctc
     5701 ccaccgagat ctcaggtggt tctgcttaaa catggtactc tgcagcaaca aaaaaaacat
     5761 ttgatgaatt ttagtttttc tgccactccg tcattgttta tcgagaaaaa attattggat
     5821 gatatagggt atctcgataa aaaatatcgg ctaatggaag attatcctct ctggcataaa
     5881 ataactgatt atggtgaaag aatttatttt gttccagaga tcactgtgtt gtatagagtg
     5941 caggaatcag ttagtaggtc aaaggagaag attgtaaatc tagatttttt aacagatatt
     6001 ctaagatttg aaaagatatt aatcaaaagg ttatctccag ttagtattac ttatcataga
     6061 agaaaactat ggtgctattt gtacccatta ttaattagac ttgttaataa taaaaaaaat
     6121 atattttcta gaggctgtat ttttctacta aatgtaattc ttaaaccatt atatttgtca
     6181 acaatagtta gacgatattt tgaaaaataa aaaggtttca tagtgatgga taataataaa
     6241 catacggttg ctgtcattat gtcagtttat gatggagatg agccgtacta ttttgaaaaa
     6301 gctattcaga gtatccaaaa acaaacctac caaagtatcg atatatattt atatatcgat
     6361 gcagtgcaaa ggaaagacct tctgaacagg attcgacact ataaaaaatg ctctaggttt
     6421 catgttgtaa caggtgataa aaaaagaggt ttagcatatg ggttaaatcg tcttttagac
     6481 aaaatccgag gaaaaggata tggatatatt gccagaatgg attctgatga tatttctacg
     6541 actgatcgca ttgaatctca ggtgagattt ctagaaaaaa ataaaaacat agatgttata
     6601 ggtacaaatt gtatagaaat agatggggat gataatgaga ttttttataa aaaaatgcca
     6661 gaaactcatc atgaactaat aaaatccata gttaagcgct ctccatttat tcatccttca
     6721 gttatgttta gaggaagtgt tgttgagcac attagatata atgatagtct tatgaatacg
     6781 caagattatt atttgtggat tgatttatta tctaaaaaat ataaatttca taatatccaa
     6841 caaccattat tattttttcg tgttaatgat tcattttacg ctagacgtgg gtttggaaaa
     6901 ttaatcaatg aagtgaaaag tagattgtac gcgatgaaga agttaaataa aaaaaatgtt
     6961 aaaaattatt tatacattct agcgttaata tgtttacgcc tcgcaccggg taatataaag
     7021 aaatattgtt acatgaggtt ccgataatga attgtatcat gtttttttat ggtgtttttt
     7081 tcgcattgcc gttatctatt ctcaacgtgt taccttatgg tatgaaaata gatgatgtaa
     7141 ttctttttat tgcattaatt gtatttccaa ttttaagttt taaaaaaggt tatcatgtga
     7201 caattccatt gtcatgttta ctatttatta tcataactgt gctgtttgcc tcactgtctt
     7261 ttttgaaggg aataaatagc acgaatgtat ttttagggga ttctgcggga actgttattg
     7321 gtcgtataat tcaatctcta cttattctca tattgattac ttttttaaaa aacaccccgc
     7381 attatttatt aagttttttt aaaggttatg ttatagctgc aaccttatcc ctcattattt
     7441 tttttggtta ttatgttacg catataaata tatcttcatt tactatgaga ggggtatatt
     7501 tcgctaagga tatttttcaa tacaatgatg acttaccctt tactgttcat gtgaacacat
     7561 taggttcttt ttttctaata gcgttttttt attaaagtat aacttcccta aataccaatt
     7621 attatcttat ttgtttttat tgccttcact actattaatc tccaaaggtg atatgttggc
     7681 aatattaact tattttgctt atgtgtttta taagagaaat aatttaaatg ctttcttatc
     7741 tctcattggt ctattgatcc ttgtattatt gatgccatat ttatacaatg tctacttaag
     7801 cttatctgat tatagagtat atgcatcagg tagagatgag ctatatggtg cgtcactatc
     7861 tagtataata gataatccat ttgggtatgg tctgggaatg caaaataata tactatattc
     7921 gataacaggc attgactttc ctgcgcataa tattctttta agtataggga ttgaacttgg
     7981 ccttctatat ttatttttgt tggttgcata tataatcggc tggttattat taggcaaagt
     8041 agattataaa atcatattta tttctttttt agtaatcgga atgtttggta atgcaatgta
     8101 tttttataag tttcattcac ttgctatagc gctttgtttt ctgagtgtat ggcatatcaa
     8161 ttttaatcac aaaactaaac aagttgattt aattaataat gaaaatactt tacattctta
     8221 ataaatctgt gctgacagga ccaaatatcg ttgcattaac aaacattgaa catattcatg
     8281 atcagggata tgatgtttct atttgttttt taaataacgg tgacgatcta aataatatat
     8341 ttcctttttt aaaagaaatt gaagttttct atcttaacct aaaatccctt ggttttattt
     8401 ctgggctaaa aaatttaaat tattatatta attcaattaa accaacaata attcacagcc
     8461 attgctttct acctgacatt tataacatat ttaataagtt tttttgcggg ccaataaaaa
     8521 ataagcgtgt tactacaatt cacaacatac ctaaagaaga ttacagatta cgatatggtc
     8581 gtattaaagg aagtcttttg ctttattttc ataaggcact tttccctttt tttgataatt
     8641 gcatatgtat ctcaaaaact gtacaaaaga gtattgggat taaaaaaaca agtgtgattt
     8701 ataatcctgt tcgtgatgtt ttttttaact caacgaaaga tgatgtagaa tgtttaacaa
     8761 ttgtatactg tggtcatttc tctcaattaa aaaaccctat tacattaatt cagttgttag
     8821 ccgattcaaa agtgaatttt cagtttttag gaatcggtga tggtcctctt ttatatcaat
     8881 gtagacaact ggttaaaaaa gatccgcgtt ttacttttgt cggtagagta aatcatgtcc
     8941 ataaatttta tgagaaagca aattgtttaa tacacatttc acaaactgaa ggtttttgtc
     9001 tttcagttgc tgaggctctg gcttctaata tgtatgtcat tacaaatgaa ttacctgttt
     9061 ttttagaact aaagcaagct ttgagttcag agggctttta catgcttaat gatataaata
     9121 attacaattt agaaatggta ttaaattcta ttaatagaaa aattgaagca ggtgaaagat
     9181 gtaattctgg tgcgtcgaat agagttaaag ctttactttc tcctactttt atagcacagc
     9241 aacatattgt gctttatcaa aaattgacag tggaaaagtg agatttcata atttttgtac
     9301 tcgtatctat attattggat gttgtcaact tgtagcgtag tttagattca tgcccccctg
     9361 acaggagtaa acaatgtcaa agcaacaaat cggcgtcgtc ggtatggcag tgatg
//

</pre>