<pre>LOCUS       KJ534585               12203 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain O68:K-:H4 O-antigen gene cluster, partial
            sequence.
ACCESSION   KJ534585
VERSION     KJ534585.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12203)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12203)
  AUTHORS   Liu,Y., Yan,X., Fratamico,P., DebRoy,C. and Needleman,D.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2014) ERRC-ARS-USDA, Eastern Regional Research
            Center, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA
FEATURES             Location/Qualifiers
     source          1..12203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="O68:K-:H4"
                     /db_xref="taxon:562"
     misc_feature    <1..>12203
                     /note="O-antigen gene cluster"
     gene            106..1191
                     /gene="rmlB"
     CDS             106..1191
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="AIT92157.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPHAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNNEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            1191..2090
                     /gene="rmlD"
     CDS             1191..2090
                     /gene="rmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="AIT92158.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQEADATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIALALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            2148..3026
                     /gene="rmlA"
     CDS             2148..3026
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="AIT92159.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSSRGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            3031..3573
                     /gene="rmlC"
     CDS             3031..3573
                     /gene="rmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="AIT92160.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHFQRPPFEQAKLVRCIVGEVFDVAVDIRVGSDSYGHWVGII
                     LSSENKRQLWIPEGFAHGFLVLSDEAEFVYKTTNYYCPEFEAGISWCDGFLNINWPQL
                     DEIKLSLKDKQWGGINAFKS"
     gene            3588..4799
                     /gene="wzx"
     CDS             3588..4799
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O antigen flippase"
                     /protein_id="AIT92161.1"
                     /translation="MLKNIFFLFLIQISNYIFPLLTLPYLVRVLGASQFGSLMLAQAL
                     IQYFITVTEYGFNLSATKKIALSKSQDETNKIYTNTLYARIILALICFIIAILCTQFI
                     EMFEREFNVIIILFLGVIGNCFFPIYLFQGLELMKKIAWISIVSKTFMTLSVFVLVKD
                     AKDINNAALALAIPLLLPGIISFIYIYNRQLARVVSFSFRGIVKELKDGGALFISQIA
                     ISFYTTFNTLILGYIFNPTIVGVFSAADKLRIAAQSCYVPIQQVVFPRINKEAGELST
                     KLLKYGGFFVLISFLGSSLVFFFGEQLALLYLGNEFIISARLFKWMSLLLFIISIAIV
                     FGQWGLITLGKEKLLTKIYVIGALCHVGYSIPLVMGYNVYGMLVSVLITEFIITLMMI
                     FSFYKVTHSAI"
     gene            4807..5757
                     /gene="wekA"
     CDS             4807..5757
                     /gene="wekA"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="AIT92162.1"
                     /translation="MVYIIVLNWNNSEDTISCISTLLKQNYIYFRIVLVDNDSTDDSL
                     IKILAFLKKSCQNYLHLSESESYLDEEIHTNKITVISATSNKGYAGGNNIGIKYALQS
                     NDCKYIWVLNNDTEVESNSLSLLVSEMERDKEIGILGSKLVYYHNKNVIQGLGGKYLP
                     WRFLTQHIASNHLITSKYDNDTISKQIDYIIGAALFVKAEVFKTVGLLDEQYFLYFEE
                     LDICLRARKHNYKLKTITDSIVYHKEGATISKEKNAFSEYHYLRSNKLFIRKFYPQTL
                     FLYNMMLIAKIANRVRRGQFDLAKANLKVLLGIKYVNSKT"
     gene            5738..6826
                     /gene="wbcC"
     CDS             5738..6826
                     /gene="wbcC"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="AIT92163.1"
                     /translation="MLTQKHEMKIDNMGNNIKKVLFFNPLKKIMIRGISVYSGEYEQL
                     LRDKNVEVIVYELPLWFGKMPKAMQVFGFMFAQQVLLPIRALKVKPDLIFDAYNTYSI
                     LAALKFNYIFVIHDFIPFNNKLWFLKPGALYQKILHNLSKNFSKLHLCYINSRIINDK
                     NAIIKKKYFILPNIVRSLGYHKVSNVEIENFIKAGRELDAICIVTISGSGKNKDFGGL
                     VERLNNLNVPVYLVAFGFANNFCEEFNNVNVFSPGVVDSNLIGSVITNSDLFVFHSTS
                     EGFGRPIIEALELKAKVICSDVSPSLDYINEEYLPNLYIYSLRDMNEFKYQFDKALRT
                     PKRLIQSDSVLTYNDEDLFNEISRIIEA"
     gene            6816..7934
                     /gene="wzy"
     CDS             6816..7934
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O antigen polymerase"
                     /protein_id="AIT92164.1"
                     /translation="MRPEMLYLTNLIFLYQIILTVLLFLPSSIFSGLIIEYTIPLLFI
                     FVMSATIYFITKRGNSFKYCQISICLPSKLILQCFFFFFIALNFSEFIFVATHGGHQA
                     LYMADRPLFPFLIFKLLAFPAFYFATIIILTKNYVVSRLLVWLALFSPLLTGSRGMTI
                     FLLLAYLFVTFGPFFFIRFKFAILGFLMIISFIGIGYYREPINMSIADYIILVVSSLN
                     EFAFASMNISNCQIPFSNVFTQFGDIFLGAKSDFRVATLLTECVSPGASEKGYGISSS
                     IVGESKILGDNLWFLYFIAILSINSIMISILLRSGSRLSHMVGISLLPFILYSVRTEI
                     VYPYVFLIKILIYILIAMIINQILVKSSAVNYKQTWGN"
     gene            7936..9117
                     /gene="wfaV"
     CDS             7936..9117
                     /gene="wfaV"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase group 1 family"
                     /protein_id="AIT92165.1"
                     /translation="MKKILVLTPRFPYPVIGGDRLRIYKICEELSKRYSLTLLSLCEN
                     NDEMSYHPDDGVFTDIIRIKQSKILSYLNVLKALISGKPLQIAYYKNRHYKNKLKHII
                     DDYDATLSHLIRVGDYVKDMSGIHILEMTDAISMNYERVRQTAPSVSLKSILYSIEYK
                     RLFNYEKEITKKFALTSIISEVDKNYLSPSFNENVIVCGNGVDFDKFRFLNRRIERED
                     KINLVFIGNMYSLQNLDGVIWFIDNIFQELNINQEYRFYVLGKIKDQDKKRLEKYMDV
                     IVSGTVEDIINEASVGHVGICPIRLGAGIQNKVLEYMALGMPVITSRVGYEGIGANIN
                     EEILIADNIDEYKSALEIIKDDIEYKNIAKKARLFVENKFRWSQQLSTLTDNIEELLK
                     K"
     gene            9114..10538
                     /gene="cpsB"
     CDS             9114..10538
                     /gene="cpsB"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="AIT92166.1"
                     /translation="MIIPIIMAGGNGSRLWPLSRTLYPKQFLSFDNRHSMLQETLLRL
                     DGLQCSPPLIICNEEHRFIVAEQLRGLNLLSNNIILEPVGRNTAPAIALAAFSIHKNK
                     PDEDPCMLVLAADHSIKNDMLFRKVVEDATKYALLGRLVTFGIVPTTAETGYGYIHRG
                     QSMIDCNNDFAFKVDKFVEKPTLEVAQRYIESGEYYWNSGMFLFRVSRYLEELKKYRP
                     DIYEACDKATSCIDYDLDFIRVNESEFRSCPDESIDYAVMEHTEDAVVIPMDVGWNDV
                     GSWSSLWSISDKNADGNSCYGDVISHSTKNSYIYSESCLVTTLGVEDLIIVQTKDALL
                     VANKNSVQDVKKIVEKIKNEGRQEHFVHREVYRPWGKYDSIDEGSRYQVKRITINPGE
                     GISLQMHYHRSEHWIIVAGTAKVTLDGEVRLLTENESIYIPSGSPHCLQNPGKIPLNL
                     IEVRSGSYLEEDDIIRIRDENGTV"
     gene            10628..11998
                     /gene="manB"
     CDS             10628..11998
                     /gene="manB"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="AIT92167.1"
                     /translation="MNDLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
ORIGIN      
        1 tggtagctgt aaagccaggg gcggtagcgt gcattaatac ctctattaat caaactaaga
       61 gccgcttatt tcacagcatg ctctgaagta atatggaata aaagagtgaa gatacttgtt
      121 actggtggcg caggatttat tggttctgct gtagttcgtc acattataaa taatacgcag
      181 gatagtgttg ttaatgtcga taaattaacg tacgccggaa acctggaatc acttgctgat
      241 gtttctgatt ctgaacgcta tatttttgaa catgcggata tttgcgatgc tgctgcaatg
      301 gcacggattt ttgctcagca tcagccgcat gcagtgatgc acctggcagc tgaaagccat
      361 gttgaccgtt caattacagg tcctgcggca tttattgaaa ccaatattgt tggtacttat
      421 gtccttttgg aagccgctcg caattactgg tctgctcttg atagcgacaa gaaaaatagc
      481 ttccgttttc atcatatttc tactgacgaa gtctatggtg atttgcctca tccagatgaa
      541 gtaaataata acgaagaatt acccttattt actgagacga cagcttacgc accaagcagc
      601 ccttattccg catcaaaagc atccagcgat catttagtcc gcgcgtggaa acgtacctat
      661 ggtttaccga ccattgtgac taactgttcg aataactacg gaccttatca ctttccggaa
      721 aaattgattc cactagtaat tcttaatgct ctggaaggta aggcattacc tatttatggc
      781 aaaggggatc aaattcgtga ctggctgtat gttgaagatc atgcgcgtgc gttatatacc
      841 gtcgtaaccg aaggtaaagc gggtgaaact tataacattg gtggacacaa cgaaaagaaa
      901 aacatcgatg tagtgctcac tatttgtgat ttgttggatg agattgtacc gaaagagaaa
      961 tcttatcgtg agcaaattac ttatgttgcc gaccgcccgg gacatgatcg ccgttatgcg
     1021 attgatgctg agaagattag ccgcgaattg ggttggaaac cacaggaaac gtttgagagc
     1081 gggattcgta aaacagtgga atggtacctg tccaatacaa aatgggttga taatgtgaaa
     1141 agtggtgcct atcaatcgtg gattgaacag aactatgagg gccgccagta atgaatatcc
     1201 tccttttcgg caaaacaggg caggtaggtt gggaactaca gcgtgctctg gcacctctgg
     1261 gtaatttgat tgctcttgat gttcactcca ctgattactg tggtgatttt agtaatcctg
     1321 aaggtgtagc tgaaaccgta agaagcattc ggcctgatat tattgtcaac gcagcagctc
     1381 acaccgcagt agacaaagca gaatcagaac cggagtttgc acaattactt aacgcgacga
     1441 gtgtcgaagc gatcgcgaaa gcagccaatg aagtcggcgc atgggttatt cactactcta
     1501 ctgactacgt atttccgggg accggtgaaa taccatggca ggaggcggat gcaaccgcac
     1561 cgctaaatgt ttacggtgaa accaagttag ctggagaaaa agcattacag gaacattgcg
     1621 cgaagcatct tattttccgt accagctggg tatacgcagg taaaggaaat aatttcgcca
     1681 aaacaatgtt gcgtctggca aaagagcgtg aagaattagc cgttattaat gatcagtttg
     1741 gtgcgccaac tggcgcagag ttgctggctg attgtacggc acatgccatt cgtgtagcac
     1801 tgaataaacc ggaagtcgca ggcttgtacc atttggtagc cagtggtacc acaacctggc
     1861 acgattatgc tgcgctggtt tttgaagagg cgcgcaaagc aggcattgcc cttgcactca
     1921 acaagctcaa cgcagtacca acaactgcct atcctacacc agctcgtcgt ccacataact
     1981 ctcgccttaa tacagaaaaa tttcagcaga actttgcgct tgtcttgcct gactggcagg
     2041 ttggcgtgaa acgaatgctt aacgaattat ttacgactac agcaatttaa tagtttttgc
     2101 atcttgttcg tgatggtgga gcaagatgaa ttaaaaggaa tgatgaaatg aaaacgcgta
     2161 aaggtattat tttagcgggt ggttctggta cacgtcttta tcctgtgact atggctgtca
     2221 gtaaacagct attacctatt tatgataaac cgatgatcta ttacccgctc tctacactga
     2281 tgttagcggg tattcgtgat attctgatta ttagtacgcc acaggatact cctcgttttc
     2341 aacaactgtt gggtgatggt agccagtggg ggctaaatct tcagtacaaa gtgcaaccga
     2401 gtccagatgg tcttgcgcag gcatttatca tcggtgaaga gtttattggt ggtgatgatt
     2461 gtgctttggt tctaggtgat aacatctttt acggtcacga tctgccgaag ttaatggacg
     2521 cagctgttaa caaagaaagt ggtgcaacgg tatttgccta tcacgttaat gatcctgaac
     2581 gctacggtgt cgttgagttt gataaaaacg gtacggcaat aagcctggaa gaaaaaccgt
     2641 tacaaccaaa aagtaattat gcggtaaccg ggctttattt ctatgacaac gacgttgtcg
     2701 aaatggcgaa aaaccttaag ccttcttccc gtggtgaact agaaattacc gatattaacc
     2761 gtatttatat ggaacagggg cgtttatctg ttgccatgat gggccgtggt tatgcatggc
     2821 tggacacggg gacacatcaa agcctgattg aggcaagcaa cttcattgct acaattgaag
     2881 agcgtcaggg gttaaaagtt tcctgtccgg aagaaattgc ttaccgtaaa gggtttattg
     2941 atgctgagca ggtgaaagta ttagctgaac cgttgaagaa aaatgcttat ggtcagtacc
     3001 tgctaaaaat gattaaaggt tattaataaa atgaacgtaa ttaaaacaga gattccagac
     3061 gtattaattt tcgaaccgaa agtttttggt gatgagcgtg gtttcttttt tgagagcttt
     3121 aaccagaagg tttttgagga agctgtaggc cgcaaagttg aatttgttca ggataaccat
     3181 tcgaagtcta gtaaaggtgt actccgaggg cttcactttc aacgtccgcc ttttgagcaa
     3241 gcaaagttgg taagatgtat cgttggtgaa gtttttgatg tcgcagtgga catcagagtt
     3301 ggttctgatt catatggtca ttgggttggt ataatccttt cctcggaaaa taaacggcaa
     3361 ttgtggatac cagaaggatt tgcgcatggt tttctggttc taagtgacga agctgagttt
     3421 gtatataaaa ctactaatta ttattgtcct gaatttgaag cgggaataag ttggtgtgat
     3481 ggttttttga acataaattg gcctcagtta gatgaaatta aactttcatt gaaagataag
     3541 cagtggggtg ggataaatgc atttaaaagc taacgcgagt ctgattaatg cttaaaaata
     3601 tattctttct ttttttgatt cagatttcta attatatttt tcctttgctt acactgccat
     3661 atttagtaag ggtcttagga gcttctcaat tcggttcttt aatgttagca caagcattaa
     3721 tacagtactt tattactgta acagagtatg gttttaatct tagtgccaca aaaaaaatag
     3781 ccttgtctaa atctcaggat gagactaata aaatatatac aaatacatta tatgctagaa
     3841 taatattggc gttgatatgt tttattatag ctattttatg tacgcaattt attgaaatgt
     3901 ttgagcgtga atttaatgtt ataataatac tattccttgg tgtaattggt aattgttttt
     3961 tccctattta tcttttccaa ggactggagt taatgaaaaa aatagcttgg ataagtattg
     4021 tttcaaaaac atttatgacc ttaagtgtat ttgtgttagt caaagatgca aaagatataa
     4081 ataatgctgc attagcgtta gcaatacctt tattactccc cggaattatt tcttttattt
     4141 atatttacaa tagacagtta gcaagagtag tttcgtttag ctttaggggt attgtaaaag
     4201 agttgaagga tggaggggcc ctgtttattt cacaaatagc tattagtttt tatacgacat
     4261 ttaatacttt aattctgggg tatattttta acccaactat cgtaggcgtt tttagcgcag
     4321 ctgataaact aagaatagct gcgcaatcat gttacgtgcc aattcaacag gtagttttcc
     4381 caagaataaa taaggaagca ggagaacttt caacaaagtt gttaaagtat gggggatttt
     4441 ttgtattaat atcattcttg ggatcttcat tggttttctt ttttggagaa caattagcac
     4501 tattatatct aggtaatgaa tttattatat cggcaagact ctttaaatgg atgtcacttt
     4561 tgttgtttat tattagtatt gcaatagtat ttggtcaatg gggattaata acattaggga
     4621 aagaaaaatt acttacaaaa atttatgtta taggtgcact atgtcatgtt ggctactcta
     4681 ttcctttagt tatgggctac aatgtttatg gcatgttagt ttcagttctt attactgaat
     4741 ttatcatcac actaatgatg atatttagtt tttataaggt tactcattcg gcgatatgag
     4801 atagaaatgg tatatataat tgtattaaat tggaataatt ctgaggacac aatttcttgt
     4861 atttcgacac tgctaaaaca aaactatata tattttagaa ttgttttagt ggataatgat
     4921 tctactgatg attcattaat aaaaatattg gcattcctaa agaaatcgtg ccaaaactat
     4981 ttgcacttaa gtgaatctga atcatattta gatgaggaaa ttcatacaaa taaaataacg
     5041 gtcatttctg caactagtaa taaaggttat gccggcggaa ataatattgg cattaagtat
     5101 gcgcttcagt caaatgattg taaatatata tgggtattaa ataatgacac agaagttgaa
     5161 tcgaattcat tatcactgtt agtgagtgaa atggaacgag ataaagaaat tggaatttta
     5221 gggagcaagt tagtttatta tcataataaa aatgtcattc aaggcttagg ggggaaatat
     5281 cttccatggc gctttttaac acagcatatt gcctcgaatc atttaataac ttctaagtat
     5341 gataacgata ctatctctaa gcaaatagat tatattatag gtgctgcttt atttgttaag
     5401 gctgaggtat ttaagactgt agggctctta gatgaacaat atttccttta tttcgaagag
     5461 ctagatattt gtttgcgagc aagaaaacat aactataaac taaaaacaat tactgacagc
     5521 atagtatatc ataaagaagg cgcaacaata tctaaagaaa aaaatgcctt ctcagaatat
     5581 cactatcttc gtagtaacaa gttgtttata cgcaaatttt atccacaaac attgttttta
     5641 tataatatga tgttaatagc taaaattgca aatagagttc gtagaggtca gtttgatctc
     5701 gcaaaagcta acttaaaagt ccttcttgga ataaaatatg ttaactcaaa aacatgagat
     5761 gaagattgat aacatgggaa ataatattaa gaaagttctt tttttcaatc cgttaaagaa
     5821 aattatgatc cgtgggattt cagtatactc tggtgaatat gagcaactat taagagataa
     5881 aaacgttgaa gttatagtgt atgaattacc attatggttt ggtaaaatgc caaaggctat
     5941 gcaagtattt ggttttatgt ttgcccaaca agtgctattg cctattcggg ctttaaaggt
     6001 taagccagac cttatttttg atgcttataa tacttattct atattagcag cgttaaaatt
     6061 taattatatt ttcgtaattc acgactttat accttttaat aataaacttt ggtttttaaa
     6121 accaggtgct ttatatcaaa aaatattaca caatttgtct aaaaactttt ccaagttgca
     6181 cctgtgttat ataaacagta gaattataaa tgataaaaat gcaattatca aaaaaaaata
     6241 ttttatatta ccaaatatcg ttagatcatt gggatatcat aaggtttcta atgtagagat
     6301 tgaaaacttc atcaaggcag ggagagagct tgatgcgata tgtatcgtaa ctatctcggg
     6361 ttccggtaaa aataaagact ttggtggttt agttgaaaga ttaaataacc ttaacgttcc
     6421 tgtatattta gttgcttttg gttttgccaa taatttttgt gaagaattta ataatgttaa
     6481 tgttttttca ccaggagttg tggattcgaa tttaattggt tcggtaataa caaatagcga
     6541 tttattcgtg tttcactcaa ctagtgaagg ttttgggaga ccaattattg aagcacttga
     6601 attaaaggct aaagtaatat gttcagatgt ttctccttcc cttgattata ttaatgaaga
     6661 gtatctgcct aatctctata tctatagttt aagagacatg aatgaattca agtatcaatt
     6721 tgataaagca ctacggacgc caaaaaggtt aattcaaagt gactcagttt tgacttataa
     6781 tgacgaggat ttattcaatg aaatatctcg aataattgag gcctgaaatg ttgtatttaa
     6841 ctaatctgat atttctttat caaataatat tgacagtatt actttttctc cctagttcta
     6901 ttttttcagg attaattata gagtacacaa tacccttatt attcattttt gttatgtcag
     6961 cgacaatata cttcatcaca aagagaggga actcatttaa atattgtcaa atttctatat
     7021 gcttaccttc aaagcttata cttcaatgtt tctttttttt ctttattgca ttaaactttt
     7081 ctgaatttat ctttgtagct actcatggtg gtcatcaagc tttgtatatg gctgatcgac
     7141 ctttgtttcc ttttttgatc tttaagctat tagcattccc tgctttttat ttcgcgacaa
     7201 ttattattct tactaaaaat tatgtggttt caagattgtt agtttggctt gctctattca
     7261 gtcctctgtt gacaggtagt agaggaatga ccattttttt attattggcc tatctatttg
     7321 ttacttttgg gccatttttc tttatcagat tcaaatttgc cattcttggt tttttaatga
     7381 ttattagctt tattggtata ggttattatc gtgaaccgat taatatgagt atagcggatt
     7441 atataatatt ggttgtatca tctttaaatg agttcgcatt tgcgtctatg aatatttcta
     7501 attgccaaat ccctttttca aatgtgttta ctcaattcgg tgatatattc ttaggagcaa
     7561 aaagtgactt tcgagttgct actcttctga ccgaatgtgt ttcccctggc gctagtgaga
     7621 aaggttatgg catttcatcc agcattgtag gcgagtctaa gatacttggt gacaatttat
     7681 ggtttcttta ctttattgct atcttgtcta taaattcaat tatgatatcg attttgttgc
     7741 gatctggctc acgtctttcg catatggttg gcatttcatt attaccattt atcctctatt
     7801 cagtacgcac agagattgtt tatccttatg tttttttaat aaaaatcctt atttatatct
     7861 taatagctat gataattaat cagattttag taaaaagcag tgctgtaaat tacaagcaga
     7921 cgtggggaaa ttagaatgaa gaagattctg gttcttaccc cccgctttcc ttatcctgta
     7981 attggtggag atcgtcttag aatctataaa atttgcgaag aattatcaaa gcgatattca
     8041 ttaacattat tatcgttatg tgaaaataat gatgaaatgt cttatcatcc ggacgatggt
     8101 gtttttactg atattattag aattaaacag tcaaaaatat tatcatattt gaacgtatta
     8161 aaagctttga tatcaggtaa gcctttgcaa atagcatatt ataagaatag acattataaa
     8221 aataaattaa agcatataat agatgattat gatgcaacgt tgtcacatct tattagagtt
     8281 ggtgattatg taaaggatat gagtggtatt catattctcg aaatgactga tgcgatctca
     8341 atgaactatg aaagagtcag acaaactgct ccttccgttt ctttaaaaag tattctttat
     8401 tctattgaat ataaacgttt gtttaattat gaaaaggaaa tcaccaaaaa gttcgcgctt
     8461 acttctatta tatctgaagt tgataaaaac tatctatcac catcatttaa tgaaaacgta
     8521 attgtgtgtg ggaatggagt tgattttgat aaatttcgtt ttttaaatcg tagaatcgaa
     8581 agagaagata aaatcaatct cgtttttata ggtaatatgt attccttgca gaatcttgat
     8641 ggggtgatat ggtttattga taatatattt caagagttaa acataaatca agaatataga
     8701 ttttacgtat tagggaaaat caaggatcaa gataagaaac gtcttgagaa atatatggat
     8761 gttattgtca gcggtactgt agaggatatt ataaatgaag ccagtgttgg tcatgtagga
     8821 atatgcccta taagactcgg agctggtatc caaaataaag tattagagta tatggcgctc
     8881 ggtatgccgg tgataacatc tagggtgggt tatgaaggta ttggtgctaa tattaatgaa
     8941 gaaatattaa ttgctgataa tattgatgaa tataagtctg ctctggaaat aattaaagat
     9001 gatatagaat ataaaaatat agcaaaaaaa gctcgtcttt ttgttgagaa taaatttagg
     9061 tggtctcaac aattatcaac gcttaccgat aatatcgagg agttattaaa aaaatgatta
     9121 tacccataat tatggctggt ggtaatggta gtcgtctatg gccgctttca agaacattat
     9181 atccaaaaca gtttctcagt ttcgataata gacattcgat gttgcaagaa accctgctta
     9241 gattagatgg cttacaatgt agtcctcctc tgattatttg taatgaagag catcgtttca
     9301 ttgttgcaga acaactccgt ggtttaaatc tgttatcaaa taatattatt ttagaacctg
     9361 tcggtaggaa cacagcacct gctatagcct tagcagcttt ttctatccat aaaaataaac
     9421 cagatgaaga tccttgcatg cttgttcttg ctgctgatca ttctatcaaa aatgatatgt
     9481 tattccgtaa ggtggttgaa gatgctacta aatatgcact tttaggtcgc ttggtaactt
     9541 ttggcatagt acctacaacc gcagaaacag ggtatgggta tatccatcgt ggtcagagta
     9601 tgattgactg taataatgat tttgctttta aggttgataa atttgttgaa aagccaactt
     9661 tagaagtagc tcaaagatat attgaatctg gtgagtatta ttggaatagt gggatgtttc
     9721 ttttccgagt tagtcgttat ctggaagagt taaaaaaata tcgtcctgat atctatgaag
     9781 catgtgataa agcgactagt tgtattgatt acgacttgga ttttattaga gtcaatgaat
     9841 cggaatttcg atcctgtccc gatgaatcta ttgactatgc tgtgatggag catactgagg
     9901 atgctgtggt cattcctatg gatgtgggat ggaatgatgt tggttcatgg tcttctctat
     9961 ggagtataag tgataaaaat gctgatggaa actcatgtta tggagatgtc ataagtcatt
    10021 caacaaagaa ttcttatatt tattctgagt cttgtttagt aacaactttg ggagtagagg
    10081 atcttattat cgtacaaact aaagatgcct tattggttgc caataaaaac tcagttcaag
    10141 atgtcaaaaa aattgtagaa aaaatcaaaa atgaaggacg tcaagagcat tttgttcacc
    10201 gcgaagttta tcggccttgg gggaaatatg attctatcga tgaaggatct cgttatcagg
    10261 taaagagaat tacaattaat ccaggtgaag gtatctcgtt gcaaatgcat tatcaccgtt
    10321 cagaacactg gatcattgtt gctggtacag caaaagtcac gctcgatggt gaagttcgtt
    10381 tgcttactga gaatgagtct atttatattc cttcaggatc gcctcactgt ttacaaaatc
    10441 ctgggaaaat ccccctcaat cttattgaag tacggtcggg ttcatatctt gaagaggatg
    10501 atataattcg tattagagat gaaaacggga cagtttaatt atcatcaata cgatgaacga
    10561 aatttggtaa ttataattca ataatattta tgattaaagt atcaatatta tattgagagg
    10621 gtgaaacgtg aatgatttaa cctgctttaa agcctatgat attcgcggga aattaggcga
    10681 agaactgaat gaagatattg cctggcgcat tggtcgcgcc tatggcgaat ttctcaaacc
    10741 gaaaaccatt gtgttaggcg gtgatgtccg cctcaccagc gaaaccttaa aactggcgct
    10801 ggcgaaaggt ttacaggatg cgggcgtcga tgtgctggat attggtatgt ccggcaccga
    10861 agagatctat ttcgccacgt tccatctcgg cgtggatggc ggcatcgaag ttaccgccag
    10921 ccataacccg atggattaca acggcatgaa gctggtgcgc gaaggggctc gcccgatcag
    10981 cggtgatacc ggactgcgcg acgtccagcg tctggctgaa gccaacgact ttcctcccgt
    11041 cgatgaaacc aaacgcggtc gctatcagca aatcaacctg cgtgacgctt acgttgatca
    11101 cctgttcggt tatatcaatg tcaaaaatct cacgccgctc aagctggtga tcaactccgg
    11161 gaacggcgca gcgggtccgg tagttgacgc catcgaagcc cgctttaaag ccctcggcgc
    11221 acccgtggaa ttgatcaaag tgcacaacac gccggacggc aatttcccca acggtattcc
    11281 taacccgcta ctgccggaat gccgcgacga cacccgcaat gcggtcatca aacacggcgc
    11341 ggatatgggc attgcctttg atggcgattt tgaccgctgt ttcctgtttg acgaaaaagg
    11401 gcagtttatc gagggctact acattgttgg cctgctggca gaagcattcc tcgaaaaaaa
    11461 tcccggcgcg aagatcatcc acgatccacg tctctcctgg aacaccgttg atgtggtgac
    11521 tgccgcaggc ggcactccgg tgatgtcgaa aaccggacac gcctttatta aagaacgtat
    11581 gcgcaaggaa gacgccatct acggtggcga aatgagcgcc caccattact tccgtgattt
    11641 cgcttactgc gacagcggca tgatcccgtg gctgctggtg gccgaactgg tgtgtctgaa
    11701 aggaaaaacg ctgggcgaac tggtgcgcga ccggatggca gcgtttccgg caagcggtga
    11761 gatcaacagc aaactggcgc aacccgttga ggcgattaac cgcgtcgaac agcattttag
    11821 ccgtgaggcg ctggcggtgg atcgcaccga tggcatcagc atgacctttg ccgactggcg
    11881 ctttaacctg cgctcctcca acaccgaacc ggtggtgcgg ttgaatgtgg aatctcgcgg
    11941 tgatgtgccg ctgatggaag aaaagacaaa acttattctt gagttactga acaagtaatt
    12001 cagtaatttc atataaataa gttttaaaag acggaaaagg tgagatattc agtgtggtat
    12061 agcaaaggta atgctattca ccatctctat gagtgagtta acatctatac cacatttaag
    12121 ccgcacactc ggcggtgacc accccctgac aggagtaaac aatgtcaaag caacagatcg
    12181 gcgtcgtcgg tatggcagtg atg
//

</pre>