<pre>LOCUS       KP710592               13569 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain K 11a serotype O65:K-:H- O antigen gene
            cluster, complete sequence.
ACCESSION   KP710592
VERSION     KP710592.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13569)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13569)
  AUTHORS   Baranzoni,G.M., Yan,X., Fratamico,P.M., Debroy,C., Tebbs,R.S.,
            Liu,Y., O'Connell,C.D., Swimley,M. and Matheny,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2015) Molecular Characterization of Foodborne
            Pathogens, United States Department of Agriculture, 600 E Mermaid
            LANE, WYNDMOOR, PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="K 11a"
                     /serotype="O65:K-:H-"
                     /db_xref="taxon:562"
     misc_feature    141..13462
                     /note="O antigen gene cluster"
     gene            complement(141..1157)
                     /gene="galE"
     CDS             complement(141..1157)
                     /gene="galE"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="AJR19389.1"
                     /translation="MSILITGGAGYIGSHTVLQLLRNEYDVVVIDNLINSNLESLKRV
                     EGLVGKKITTYIGDVTDSTFLKTIFDNHSVTDVIHFAGLKSVSESINDPLLYYKNNVQ
                     GSLCLITEMAKAGVYNLIFSSSATVYGSPQSVPLTEMSPLGLTTNPYGTSKLMIEQIL
                     RDFTNSNPNFKVVCLRYFNPVGADPSGCIGESPIGTPNNIFPYLTQVALGKFPYLSIF
                     GDDYPTIDGTGVRDYIHVVDLALGHLAALKFKQNETSFAVFNLGTGTGYSVLELVNTF
                     EEVTGIKIPCRIVGRRPGDVAECWSDPSLAKKVLFWEAKKTLVDMVRDAWNWQQKNPS
                     GYSS"
     gene            complement(1188..1997)
                     /gene="wbbD"
     CDS             complement(1188..1997)
                     /gene="wbbD"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-Gal:alpha-D-GlcNAc-diphosphoundecaprenol
                     beta-1,3-galactosyltransferase"
                     /protein_id="AJR19390.1"
                     /translation="MKFSVLMSVYKDDNPLYLNQSIESIWIHQTLKPTQIVLVQDGVL
                     TEALYGVVNKWKQKVGDSFTIVSLDRNMGLGAALNRGLLYCKYELVARMDSDDVALPY
                     RFQIQVEYMIKNCEIIASSGYVEEWDEEMGTLLGIRYVPLGTDSIIKMARFRSPLSHP
                     ASIFRRDIVLSLGGYPELRKAQDFALWSLLLKNKYKLSNLGTVLVKMRAGNSMIYRRG
                     FSHLRYEMELLKYQRMIGFLPFGLFCVNFVLKAGLRLSPGFVKKIAYKFLR"
     gene            complement(1994..3118)
                     /gene="pglJ_1"
     CDS             complement(1994..3118)
                     /gene="pglJ_1"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylgalactosamine-N,
                     N'-diacetylbacillosaminyl-diphospho-undecaprenol
                     4-alpha-N-acetylgalactosaminyltransferase"
                     /protein_id="AJR19391.1"
                     /translation="MKKKVLYVVSTLKRSGPINQLYNLINNFSKYEFDLTIVTLSPEP
                     QDSKLDDFINLGINVITLGLSRLKGVFFAKHKLKKIIDKINPDIVHSQGVRADYLNYL
                     IVGGHMKISTIHNYPQKDFIKRYGPIVGTVLVKIQINAIKNFDIVIGVSKAVAKNLIS
                     TYKIKNAYAVINGIDTDIYKPKSSTDKSKLKEKLGLAKYDCIWISTGHLSVLKQPEIL
                     IKSFLKNKNDARFINSVFIFLGDGELSESLIMKYKHEKKIIFAGRVHNVVEYLQASDY
                     FASASSTEGLPMAVIEALSCGLPVLLSRIGPHEEIIGLDESIGVLFDSDDASESFMTM
                     VTKDKTALSHSCRSLALNFFSAEAMGANYKDIYNKSIKTK"
     gene            complement(3120..4391)
                     /gene="wzy"
     CDS             complement(3120..4391)
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AJR19392.1"
                     /translation="MIYFTVLVMLFILGVLSLIKKSNLFSPSILFPLVIVTTLLPSLL
                     LPSVFYTPSVYSVLYIFVCTTSFSFFSFLFEQINIKSKLSCNVLSFNMSGYYFIAFVT
                     FILCFLMLDEFLNKIAEFANFGAYAEYYRAASTSGEDDLKDSVNYLVRNADMLALPLS
                     VIGLYIWSTRRVSSIFKITVVIFILFSLTLPIISVARSGAIRACIVLLGVFVIFFRFR
                     LKFAILVLSFVVALYVGAGFMFGYGSGGYSSFSESIGYFLVSFIRYLSGGYYTFDSYL
                     LGKVDIFFRFAFVDSILGKIDTTLTLAGMGGKCIVCYPENHAEFVQVGPAENFVSNVY
                     TVFGVVFSNLGWLGVFYFSLCGAIMGSLYKAMKSGRVFFAIVYSFLLPGVVLASFSEY
                     FFAQIPLLIRFILLYIILFKLSAHKNKLEGF"
     gene            complement(4388..5515)
                     /gene="pglJ_2"
     CDS             complement(4388..5515)
                     /gene="pglJ_2"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylgalactosamine-N,
                     N'-diacetylbacillosaminyl-diphospho-undecaprenol
                     4-alpha-N-acetylgalactosaminyltransferase"
                     /protein_id="AJR19393.1"
                     /translation="MDVKNKKITIFISSLRPGGAEGVCVTVANGLVKLGWDVTIVVLN
                     MIRATHQQRLDTSINIVNLNCRHAREAIFPIWKFLSSSASSNLLVFNHQLAIILVLIR
                     IFTRKNYIIISRNINTLSVDLKNAKNFWHKHIVSFLIKRFYSKVEHIIAQSHGMKDDL
                     INFLNINASKISVIHNPVNPYFSDFAKAIKWNDYDKDGFILCVGRLERQKAFHFAVDI
                     FSKVAEKRQDLNLFIIGEGSLRQELEDYVSRLKVTRKVKFLGLRNDLEKYYSKASVVL
                     LTSLYEGFPNVLVESLTLGTPVVAFDCPNGPNEIINSNNGLLVNYKDVNDGVEKLNKA
                     LSQEWDYVEIAKAASIYSEEYIVNIYSDVLKQTFYSTELLR"
     gene            complement(5506..7395)
                     /gene="asnB"
     CDS             complement(5506..7395)
                     /gene="asnB"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase [glutamine-hydrolyzing] 1"
                     /protein_id="AJR19394.1"
                     /translation="MCGIIGYIDFDKNSNADVLKKMTDSLHHRGPDDSGILDEEFNNC
                     KVGFGHRRLSILDLSSHGHQPMTFMNLSIVYNGEVYNFKEIREQLEKEHYVFNSDSDT
                     EVLLKGWHCWGEDLIQKLNGMFALAVLDRTNQTVTLLRDRAGVKPLYWYRKNNLILFS
                     SELKGLHRHNAFSKEISKEGVNLYFQYGYIPQPYTIFENVYKLESGHCLKIDLVNQAI
                     QKNCYWNVFDYYRKEKIKLTESEAIVKCESLLSSACEYRMIADVPVGVFLSGGYDSSI
                     VAALLQRNNSKKLKTFTIGFKEKSYNEAGYAKDISNYLGTEHTEYYCTSDDALNILPL
                     LPDICDEPFADPSIIPTILVSRLAKKDVTVSLSADGGDELFAGYDKYTGIKKKIDIFN
                     IIPNMAYPGIRKILETKLAHKAFEYAGYTDVNNRLFRLAQMIGQDEVRILKSASIIFS
                     DLELNHLLGGADEIYTGFDDRLNSNWLDNLLAIDYKTYQVDDILFKVDRATMSCSLEG
                     REPLLDHRLIEFVARLEPELKIKNGDRKYILKKIAHKYIPQSLLDRPKQGFGIPLSEW
                     LGGQLKNYVSYYLNPKRIAESGILNPVFVDSLCRDYYSGNINKVNFKKIWVLLMFEMW
                     REKWM"
     gene            complement(7398..8300)
                     /gene="epsE"
     CDS             complement(7398..8300)
                     /gene="epsE"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="AJR19395.1"
                     /translation="MTNNSYPLVSVAIITYNQKEYLRECIESILLQNYPNLQIVVADD
                     CSSDGTQDLLLRYSQQYPNVFTLKLSEKNLGITANSNLAHFACRGKYIFWMGGDDLML
                     PGKIAKQVEYMENNPDCTLCYHNLDVFDSATKKNLYYFNHLKKQKINGTIREIIKFRT
                     FNGACSTVVRKDKTPKEGFNELIPIASDWLYWVDTLANGGNIKYIDEVLGKYRRHDNN
                     ITKKTKCLSQADIDHLNTCNIIIGKYPQYLSEALYAYAEILFHLRKKLDYKKIVCFSL
                     GANFRIRKAIALGVFLLSFGIIKV"
     gene            complement(8314..9564)
                     /gene="wzx"
     CDS             complement(8314..9564)
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AJR19396.1"
                     /translation="MNLIRTSLLSVISTFFRLLSALVINKAIATFIGPTGLALIGQFQ
                     NFTQIALVLAQGGINNGVVKYNAELISENKDITILNSTAIRISLLSSLFIGFLMAIFS
                     NYLSDYIFNSPNYSYVFIIFGITIFFFVINQLLLSILNGLKEIRLFIGINISQSIYSL
                     IFTSLAILTYGLVGALIAMVTNQSIIFITVVWRLRKHPIISLRLFNANYDKKIANKLF
                     KYSVMALTSILTVPLTNFILRNYIGNVIGWSQAGYWQAIWYISSMYLMVITTALNIYY
                     LPRLSEIKDKKEIKKELLNGYVLIIPFIVLLTLGIFFLKEYIVLILFNKDFYPMLELF
                     KWQLVGDVIKISAWLLSYIMLAKAMTKEFIITEILFSMLFVIVSLIYINVFGLIGVTY
                     GYATNYIIYFIVMFLIFRKKYLLQ"
     gene            complement(9561..10670)
                     /gene="fdtB"
     CDS             complement(9561..10670)
                     /gene="fdtB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose
                     transaminase"
                     /protein_id="AJR19397.1"
                     /translation="MIEFLNLKKINNQYKDELKEACAKVIDSGWYVLGEHLEEFEKDY
                     SKWCGVKHTIGVANGLDALSLVLRAWKEMGMLKDGDEIIVPANTYIASILAITANRLV
                     PVLTEPDLITYNLSPKNIEKNITKKTRAILPVHLYGNISPMDDIMALASKYKLLVLED
                     SAQAHGASINGRKAGAWGNASGFSFYPGKNLGALGDAGAVTTNDDELAYVVKALRNYG
                     SHTKYKNLYEGVNSRLDEIQAAMLSVKLKYIDKEVEKRRIIARSYLKNIKNILIKLPT
                     VLDDERHAWHLFVVRTCQRERFQAYLSTNGIQTLIHYPTPPHLQDAYKNKRVTSGFSL
                     PITEMIHNEVVSLPMDPTMSDKDIEHVISTVNRFK"
     gene            complement(10675..11127)
                     /gene="fdtC"
     CDS             complement(10675..11127)
                     /gene="fdtC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose
                     3-N-acetyltransferase"
                     /protein_id="AJR19398.1"
                     /translation="MFIHELSDVQTEHIGENTRVWQYSVILPEAIIGEDCNICAHTLI
                     ENKVTIGNRVTIKSGVYIWDGVTIEDDVFIGPGVVFTNDKYPRSKRYPEQYPTIIIKK
                     GASIGANATILPGVTVGEGAMVGAGSVVTKNVEPNTIVIGNPAKELKR"
     gene            complement(11128..11523)
                     /gene="fdtA"
     CDS             complement(11128..11523)
                     /gene="fdtA"
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-4-oxo-6-deoxy-alpha-D-glucose-3,
                     4-oxoisomerase"
                     /protein_id="AJR19399.1"
                     /translation="MKIELLPLQIHGDSRGSLISLEYAINIPFDIKRVYYIFNTKEGV
                     TRGYHAHKQLKQIAIAITGSCDFTMDNGKERITLTLNNPAQGLLIDSCIWREMSNFTP
                     DCVLMILASNEYDESDYIRDYEEFKLMVK"
     gene            complement(11526..12389)
                     /gene="rmlA"
     CDS             complement(11526..12389)
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase 2"
                     /protein_id="AJR19400.1"
                     /translation="MKGIILAGGSGTRLYPITMGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIQDILIITTPEDQQGFIRLLGDGSQFGVNLTYAIQPSPDGLAQAFIIGENFIGNDTV
                     CLVLGDNIFFGQGFTPKLRDAAERTSGATVFGYQVMDPERFGVVEFDRNFKALSIEEK
                     PANPKSNWAVTGLYFYDNNVIDIAKGIKPSKRGELEITAVNEVYLRNNNLTVELLGRG
                     FAWLDTGTHDSLIEAGSFVETVQKRQGMMVACLEEIAWRNGWLSKIKLYELGHKLSKN
                     QYGQYLIKLAS"
     gene            complement(12386..13462)
                     /gene="rmlB"
     CDS             complement(12386..13462)
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="AJR19401.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADVCDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTINWY
                     LNNNDWCRRVQDGSYQRERLGVIR"
ORIGIN      
        1 catcactgcc ataccgacga cgccgatctg ttgctttgac attgtttact cctgtcacga
       61 agactgctca gaacataaat aaaaatttat ttgaagttac tctacgaaat ataaataata
      121 atccagtttg catttatgag ttaagatgag tatccacttg ggttcttttg ttgccaattc
      181 caagcatcac gaaccatgtc gaccaaagtt ttcttcgcct cccaaaaaag tacttttttg
      241 gcaagagaag gatctgacca acattccgca acatctccag gccgtctccc aacgatccga
      301 catgggatct taattcctgt cacttcttca aaagtattaa ctaactcaag aacagaatag
      361 cctgtcccag ttcctaagtt aaaaactgca aacgatgttt cattttgttt aaactttaat
      421 gctgcaagat gccccaatgc taaatctacc acatggatat agtcacgaac gcctgttccg
      481 tctatagttg ggtaatcatc tccaaaaatt gataaatagg gaaattttcc caacgcaacc
      541 tgagtcaaat aagggaaaat attatttgga gttcctattg gggattcacc tatacaacca
      601 gaaggatcag caccaacagg attaaaatat cttaggcata cgactttaaa attaggattg
      661 gaattagtga aatctcttaa aatctgctcg atcattaatt tagaagtacc atatggattt
      721 gttgtcagtc ctaatggact catttcagtc aatggtactg actgagggga accatacaca
      781 gttgctgagg aactaaatat taaattataa acccccgcct ttgccatttc agtaataaga
      841 caaagagaac cttgtacatt atttttataa tacagcaagg gatcattaat agattctgaa
      901 actgacttta acccagcgaa atgtataaca tcggtgacag agtgattatc aaaaatcgtt
      961 tttagaaacg tactatcagt tacatcgcct atataagtcg taattttttt tccaactaag
     1021 ccctcaactc gctttaaaga ctccagatta gaattaatca aattatcaat aacaacaaca
     1081 tcatactcat tccttagtaa ctgtaatact gtgtgagaac caatataacc ggctccacct
     1141 gtgattagga tggacataca ttcaccttga gttaaacatg cgctctatta gcgtaaaaac
     1201 ttatatgcaa tcttctttac aaatccggga gataaacgaa gcccagcctt taaaacaaaa
     1261 ttaacacaaa acaaaccaaa aggtaaaaac cctatcattc gttgatattt aagaagttcc
     1321 atttcatatc tcaaatggct gaatccacgt ctataaatca tactatttcc tgctcgcatc
     1381 tttactaaaa cagtccctaa atttgataac ttgtatttat tctttaataa taaagaccaa
     1441 agggcaaaat cctgtgcctt acgtaattca ggataccccc ccaaagataa aacaatatca
     1501 cgtctaaata ttgatgcagg atgactaaga ggggagcgaa aacgggccat ttttataatc
     1561 gaatcagttc ccaatggaac atatcgtata cccaataatg tacccatttc ttcatcccac
     1621 tcctcaacat aaccactaga agcaataatc tcgcaatttt ttatcatata ttcaacttga
     1681 atttgaaatc tatatggtaa tgcgacatca tcactatcca tcctggcaac caactcatac
     1741 ttgcaatata ataaccctct atttaaagct gcccccagcc ccatatttct atctagcgaa
     1801 acaatggtaa atgaatcacc taccttttgc ttccatttat ttacaacacc atagagagcc
     1861 tctgttaata caccatcttg gaccagaact atctgggttg gttttaatgt ttgatgtatc
     1921 cagatgcttt ctattgattg attcaaatat aaagggttat catctttata aacagacatt
     1981 aatactgaaa atttcattta gtttttatac tcttattata tatatctttg tagttagctc
     2041 ccattgcttc tgcactaaag aaattaagcg ccaaacttcg acaagagtga gaaagcgccg
     2101 ttttatcctt tgtaaccatt gtcatgaatg attcagatgc gtcatcacta tcgaataaca
     2161 ctccaatgct ttcatcaagc ccaattattt cttcatgagg cccaattctt gataataaga
     2221 caggtaaccc acaagataat gcttcaatta ctgccatagg taaaccttct gtactagatg
     2281 ctgatgcgaa ataatcggaa gcttgcaaat actcaactac attatgaaca cgacctgcaa
     2341 atattatttt tttttcatgt ttgtatttca taataagaga ctcactgagc tctccatccc
     2401 ctaaaaaaat aaaaacgcta ttgataaaac gagcatcatt cttatttttt aaaaacgatt
     2461 taattagaat ctccggttgt tttaatacac taagatgccc agtagatatc catatgcaat
     2521 catatttagc caaacccagt ttttctttta gtttactttt atcggtgctg gatttaggtt
     2581 tataaatatc agtatctata ccattaataa ctgcatatgc atttttaatc ttgtaagtac
     2641 taattagatt tttagcaaca gctttagaaa cacctataac aatatcgaaa ttctttatgg
     2701 catttatttg aatcttaacc agaacggtac caacaatagg gccatatctt ttaataaaat
     2761 ctttttgcgg ataattatga attgtgctaa ttttcatatg gccgccaaca attaaataat
     2821 ttaaataatc agctctaacc ccctgagaat ggacaatgtc aggattaatt ttatcaatta
     2881 tcttttttag tttatgtttg gcaaaaaata cccccttgag tctactaagc ccaagagtaa
     2941 taacattaat acccaagtta atgaaatcat ctaattttga gtcttgtggt tcgggcgata
     3001 aagttacaat cgtcaaatca aattcatatt tcgagaaatt attgatcaaa ttgtataatt
     3061 gattaattgg ccctgacctt tttagtgttg acacaacgta aagaactttt tttttcaatt
     3121 taaaatccct ccagtttatt tttatgagca gacaatttaa ataaaattat atataacaaa
     3181 atgaacctta ttagtagcgg gatttgagca aaaaaatatt cactaaatga cgcaaggact
     3241 actccaggta aaaggaaact atatacaatt gcaaaaaaga ctctaccgct tttcatagct
     3301 ttgtaaagtg agcccataat tgcaccgcat aagctaaagt aaaatactcc tagccaacct
     3361 aagttgctaa atacaacacc gaatacagta tagacgtttg aaacgaagtt ttcagctgga
     3421 ccaacctgga caaactcagc atgattttct gggtagcaga caatacactt accccccatc
     3481 ccagctaaag tcaacgttgt atcgatttta ccaagaatgc tatctacgaa tgcaaatcta
     3541 aagaaaatat caaccttgcc aagcaaatat gaatcaaatg tataataacc accagataaa
     3601 taccggataa aggataccaa gaaataccct atagattctg aaaaagatga atacccacca
     3661 gaaccatagc caaacataaa accagcgcca acatataacg caacaacaaa actcaataca
     3721 agaatggcaa atttcaacct aaatcgaaaa aatataacaa acacgccaag tagaacaatg
     3781 caagctctaa tagcacctga acgagcgacg ctaataatag gaagggttaa agagaacaat
     3841 ataaagatca caacggttat tttaaatatg cttgatactc tacgcgttga ccaaatatac
     3901 aatccaatta cagataatgg taatgctaac atatcagcat ttcttactaa ataatttaca
     3961 ctatccttta gatcatcttc accagaagtt gaggcagctc tataatattc ggcatatgct
     4021 ccaaagtttg caaactctgc aattttgttt aaaaattcat caagcattaa aaaacacaaa
     4081 ataaatgtaa caaaagcaat aaaataatac ccagacatat tgaaagataa tacattgcaa
     4141 gacaactttg atttaatatt tatttgttca aatagaaatg agaaaaaaga aaaagaagtt
     4201 gtgcaaacaa atatatacaa aacagaatat actgacggtg tataaaaaac acttggcaga
     4261 agtaagctgg gaagtaaggt tgtaacaata actaaaggaa ataatattga aggtgaaaac
     4321 agattgcttt ttttaattaa actgagtaca cctaatatga acaacatcac taaaactgta
     4381 aagtatatca tcttaaaagt tctgttgagt aaaatgtctg ttttaaaaca tcggaataaa
     4441 tgttaacaat atattcctca ctatatatac ttgcagcttt tgcaatttcc acataatccc
     4501 attcttgact caatgcttta tttaattttt cgactccgtc attgacatct ttataattaa
     4561 caagcaaacc gttattagaa tttataattt cattcggtcc attagggcaa tcaaatgcca
     4621 caactggtgt tcccaatgtt aatgattcca ctaatacatt tggaaatcct tcatataagg
     4681 aagtaagtaa caccacagag gctttactat aatatttctc aagatcattt cttagtccaa
     4741 gaaacttgac ttttctagtg actttaagcc tgctaacata atcttctagc tcctgtctca
     4801 aactcccctc acctattata aacagattca aatcttgtct tttctcagca actttcgaga
     4861 aaatatcaac cgcaaaatga aaagcttttt gtctttccag tctgcccaca cataatataa
     4921 atccatcctt atcataatca ttccatttta ttgctttagc aaaatcagaa aaataaggat
     4981 taacaggatt atggattaca gatatcttac ttgcattaat attcaagaaa ttaataagat
     5041 catctttcat tccatgtgat tgtgctatta tatgttcaac ctttgaataa aatcttttta
     5101 ttaagaaact aacgatatgt ttgtgccaaa aatttttcgc attttttaaa tcaacactta
     5161 atgtgttgat attacgagag ataattatat agtttttcct agtaaaaatt ctaatcaata
     5221 ccagaataat tgctagttga tggttaaaca ctaataaatt ggaagaggct gatgaactaa
     5281 gaaatttcca aataggaaat attgcttcgc gtgcgtgcct gcaattcaga ttaacgatat
     5341 taattgatgt atcgagcctc tgttgatgag tcgctcttat catattaagt acaacaattg
     5401 taacatccca accaagtttg acgagtccat tagcaactgt aacacaaact ccctcggccc
     5461 cccccggacg tagcgaactg ataaaaatag ttattttttt gttctttaca tccatttttc
     5521 tctccacatt tcaaacatta ataaaaccca tattttttta aaattaactt tatttatatt
     5581 tccagaataa taatctcgac ataatgaatc aacaaaaaca ggatttaata ttccagactc
     5641 agctattctt ttgggattaa gataataaga aacataattt tttaattgcc cacctagcca
     5701 ctcagaaagt gggatgccaa atccttgttt tggacgatct aacaatgatt gaggtatata
     5761 tttatgggct attttcttca aaatatattt tctgtcccca ttttttattt ttaactcggg
     5821 ctctaacctt gcaacaaatt ctattagtct atgatcaaga agcggttctc ttccttcaag
     5881 agagcaagac atagttgctc gatcaacttt aaataaaata tcatcaacct gataggtttt
     5941 gtaatcaatt gccagcaaat tatcgagcca gttgctattc aaacgatcat cgaatccagt
     6001 atagatttca tctgccccgc ctaaaaggtg attaagctct aaatcagaaa aaatgataga
     6061 tgctgatttt agtattctaa cttcatcttg ccctatcatt tgtgctaatc gaaataggcg
     6121 attattgaca tcagtataac cggcatattc aaacgcctta tgtgctaatt tagtctcaag
     6181 aatttttcta attcctggat aagccatatt aggtattata ttaaagatat caattttttt
     6241 cttaatacca gtatatttat catacccagc aaagagctca tccccaccat ccgcacttaa
     6301 tgaaaccgta acatcttttt tcgctaacct actaacaagt attgtaggga taattgatgg
     6361 atccgcaaat ggttcatcac aaatatcagg aagaagaggg agtatattta aagcatcatc
     6421 actcgtacaa taatattcag tatgttcggt acctagataa tttgaaatat cttttgcata
     6481 accagcttca ttatatgact tttctttaaa gcctatagta aatgtcttta attttttaga
     6541 attattgcgc tgtagcaatg cagcaactat agaggaatca tacccaccac ttaagaaaac
     6601 accgacagga acatcagcga tcatgcgata ttcgcaggca gagcttaata aactttcgca
     6661 ttttacgata gcttcactct ctgtaagttt gatcttttcc tttctataat aatcaaaaac
     6721 attccaataa caatttttct gtattgcttg atttacaaga tcaattttca aacaatgtcc
     6781 agactctaac ttataaacat tttcgaatat tgtatatggc tggggtatat atccatactg
     6841 aaaatataga ttaactccct cttttgatat ttctttagaa aacgcattgt gtctgtgtaa
     6901 tccttttaat tcactagaaa ataatatcaa attattttta cgataccaat agagaggctt
     6961 gactcctgct ctatcacgca ataatgtaac cgtttgatta gtgcgatcaa gtacagcaag
     7021 agcaaacatt ccattaagtt tctgtattaa atcctccccc cagcagtgcc aacctttaag
     7081 caacacttct gtatccgaat ctgaattgaa tacataatgt tctttttcta attgttcccg
     7141 tatctctttg aaattataaa cctcaccatt ataaacaata gaaagattca taaatgtcat
     7201 gggctgatgt ccatgtgatg ataaatcaag tatggataat cttctgtggc cgaaccccac
     7261 cttacagtta ttaaattctt cgtctaaaat tccactatca tctggtcctc tatggtgcaa
     7321 ggagtctgtc atttttttta gtacatcagc attcgaattt ttgtcgaaat ctatataacc
     7381 aataattcca cacatactta aaccttaata attccgaagc ttaataaaaa gacaccaagg
     7441 gctattgctt ttctaattct gaaatttgca cctaaactaa aacaaacaat ttttttataa
     7501 tccaacttct tccttaagtg aaataatatc tcagcgtaag catataatgc ttcacttaga
     7561 tattgaggat acttaccaat gataatgtta caagtattca aatgatcaat atctgcttgg
     7621 gataaacatt ttgttttctt tgttatgtta ttatcatgcc gacggtattt ccctaacact
     7681 tcatctatat attttatatt tcctccattt gctaatgtat caacccaata taaccaatca
     7741 gaagcaatag ggataagttc attaaaccct tcctttggtg ttttgtcttt tcttacaaca
     7801 gtagaacacg ctccattaaa agtcctaaac ttgataattt ctctaattgt cccattgatt
     7861 ttttgttttt ttaaatgatt gaaatagtat aaattttttt tagttgcaga gtcaaaaaca
     7921 tctagattat gatagcataa tgtacagtct ggattatttt ccatatactc tacttgtttt
     7981 gctatttttc cgggcagcat caaatcatct ccacccatcc aaaaaatata cttacctctg
     8041 cacgcaaaat gcgctaaatt agaatttgct gttattccta aatttttctc agataatttt
     8101 aatgtgaata cattagggta ttgttgactg tatctcaata ataaatcttg agtcccatct
     8161 gatgaacaat catctgctac tactatctgc aaatttggat agttctgaag taatatagac
     8221 tcaatacatt ctctaagata ttctttttgg ttatatgtga taattgcaac actcaccaaa
     8281 ggatagctat tatttgtcaa gcgcacctca ttattattgt aaaagatatt tttttctaaa
     8341 gattaaaaac atcacaataa aatatattat ataatttgtt gcatatccat aagttacacc
     8401 aattaaccca aatacattta tatatatcaa tgaaacaatc acaaataaca ttgagaacaa
     8461 tatttcggta atgataaatt ctttagtcat tgctttagct agcattatat aactcaaaag
     8521 ccaagcgctt atttttatca catcccccac tagttgccac ttaaacaact caagcattgg
     8581 gtaaaaatct ttattaaaaa gtataagaac aatgtattct tttaaaaaaa agatacccaa
     8641 cgttaataat actataaaag gtataatcag gacataacca tttaacaact ccttttttat
     8701 ttctttctta tctttaattt cagataatcg aggtaaatag tatatattga gagcagttgt
     8761 aataaccatc aaatacatac tggaaatgta ccatatcgct tgccaataac cagcttgtga
     8821 ccatccgatt acatttccaa tataattgcg aagaataaaa tttgttaatg gcactgttaa
     8881 tatacttgtc aatgccatta cagagtattt aaacaattta ttcgctattt tcttatcata
     8941 attagcatta aataatctca ggctaataat tggatgtttt cttaatcgcc acaccacagt
     9001 tataaatata attgactgat tagttaccat ggcaattaaa gcaccaacta atccatatgt
     9061 tagtattgct aaagatgtaa atattaaact ataaatactt tgcgatatat taatacctat
     9121 gaataacctt atttctttta aaccatttaa tatagacaac aaaagctgat taataacaaa
     9181 aaaaaatatc gttatgccaa aaataatgaa tacataactg taatttgggc tattaaatat
     9241 ataatctgat aaataatttg aaaagatggc cattaagaat cctatgaaca acgatgataa
     9301 caagctgatt cgaatggccg tactatttaa aatagttata tctttatttt ctgaaattaa
     9361 ttcagcatta tatttaacaa ccccgttatt tattccccct tgagctaaca caagcgcaat
     9421 ctgagtaaag ttttgaaatt gtccaatcag agctaaaccc gttgggccaa taaaagtggc
     9481 aatagcttta tttattacta aagcagatag taatctaaaa aatgtcgaga ttacgcttaa
     9541 taaagaagtc cttattaaat tcatttaaat ctattaaccg tgctaattac atgctctata
     9601 tctttatcag acatagtcgg atccataggc aaagaaacta cttcattatg tatcatttct
     9661 gtgattggta aagaaaaacc acttgttact cttttgtttt tgtaagcgtc ttgtaggtgt
     9721 ggtggggttg ggtaatgaat taatgtctgt atgccatttg tgcttaaata agcctgaaaa
     9781 cgttcacgtt gacaagttcg aacaacaaaa agatgccacg catgacgctc gtcatctaat
     9841 actgttggca gtttgattaa tatatttttt atatttttta gataactacg agctattatt
     9901 ctgcgttttt ctacttcttt gtcgatatat ttaagtttaa ctgacagcat tgctgcctga
     9961 atctcatcta gtctactatt tactccctca tataagtttt tatattttgt atgtgaacca
    10021 taatttctca aagcttttac aacatacgct aattcatcat cattagtagt aacggccccg
    10081 gcatcaccca atgcgccaag gtttttacct ggatagaagc tgaaacctga agcgtttccc
    10141 caagcaccag ctttgcgacc atttattgaa gctccatgtg cctgtgcact atcttcaaga
    10201 accagtaatt tatattttga tgccaaagcc ataatatcat ccattggaga aatattacca
    10261 tatagatgca ctggcaatat tgctctggtt ttctttgtga tatttttttc gatattttta
    10321 gggcttaggt tatatgtaat tagatctggt tcagttagaa ctggaactaa tctattagca
    10381 gttatggcca gaatagatgc tatataggta ttagctggta cgattatttc atctccatct
    10441 ttcagcatcc ccatttcttt ccaagctcgt aaaacgagag acaatgcatc caatccgtta
    10501 gccactccga tagtatgttt tacaccacac cactttgaat aatctttttc aaactcttcc
    10561 agatgttctc ctaaaacata ccaaccagaa tcaataactt ttgcacaagc ttcctttaat
    10621 tcatctttat attgattgtt tatttttttt aaatttaaaa attcaatcat cacattatct
    10681 ctttaattct ttagctggat ttccgattac tattgtatta ggctcgacat ttttggtaac
    10741 tactgaacca gcacctacca ttgcaccttc gcctacagta acaccgggta aaatagttgc
    10801 atttgcccct attgaagctc ccttttttat aattatagtt ggatattgtt cagggtatct
    10861 ctttgatcga ggatatttgt catttgtaaa aaccacacca ggaccaataa aaacatcatc
    10921 ctctatagtt acaccatccc aaatatatac gccagacttt attgtaactc tattaccaat
    10981 tgttacttta ttttctatta aagtgtgtgc gcatatatta caatcttctc caattattgc
    11041 ttcgggtaaa attactgaat actgccatac tctggtgttc tcaccaatat gttctgtttg
    11101 aacatcactt agctcatgta taaacattta ttttaccatt aatttaaatt cttcatagtc
    11161 acgaatataa tcagactcat catattcatt actcgccaaa atcattaaca cacaatctgg
    11221 agtaaagtta ctcatttctc gccaaataca tgaatcaatc aataatcctt gcgctggatt
    11281 gtttaatgtt aatgtaattc tctcctttcc attatccatc gtgaaatcac atgaaccggt
    11341 tatggctatt gcaatttgct ttaactgttt atgagcatga taacctctag taacaccttc
    11401 ttttgtatta aaaatatagt aaactctttt tatatcaaaa gggatattaa tggcatactc
    11461 taaagatatc aatgatccac gactatcacc gtggatttgt aagggtagta attcaatttt
    11521 cattgtcaac tcgcaagttt aatgagatat tgaccatact gatttttact caatttgtga
    11581 cctaactcat acaattttat cttacttaac caaccatttc gccatgctat ctcctccaga
    11641 caggcaacca tcatcccttg tcttttttgg actgtttcta caaaactccc agcttcaatc
    11701 aagctgtcat gggttcctgt atccagccat gcaaaaccac gccctagtaa ttcaacagtt
    11761 aaattattat ttctgagata aacttcatta actgccgtaa tttctaattc accacgttta
    11821 gaaggtttaa taccctttgc aatatcgata acgttattgt cataaaaata taatcctgtg
    11881 acagcccaat ttgattttgg attggcaggt ttttcttcaa tacttaaagc tttaaaattt
    11941 ctatcaaact ccactactcc aaatctctca ggatccatta cttgatatcc aaagactgtg
    12001 gcacctgatg ttctttctgc tgcatcccgg agtttgggtg taaaaccttg tccaaaaaaa
    12061 atgttatctc caagcacaag gcatactgtg tcatttccaa taaagttttc accaataata
    12121 aatgcttggg ctaaaccatc tggactaggt tgaatcgcat atgtcagatt aacaccaaat
    12181 tggcttccat ccccaagtaa tcttatgaac ccctgctgat cttctggcgt tgtaataata
    12241 agaatatctt gtatacctgc caacatcagc actgacaaag gataataaat catcggttta
    12301 tcataaatgg gtaatagctg ctttgaaact cccattgtaa ttggataaag ccttgttcca
    12361 gaaccaccag ctaaaataat tcctttcatc taattacccc caaacgctca cgttgataag
    12421 agccatcttg aactcgccgg caccaatcat tattattcaa ataccagtta attgttttac
    12481 gaatcccgct ctcaaacgtt tcctgtggtt tccatcccaa ttcgcgacca atcttctcag
    12541 catcaatggc ataacggcga tcgtgtcccg gacgatcggc aacataagta atttgctcgc
    12601 ggtaagattt ctctttcggt acaatctcat ccaacaaatc acaaatagtg agcactacat
    12661 cgatgttttt cttttcgttg tgtccaccaa tgttataagt ttcacccgct ttaccttcgg
    12721 ttacgacggt atataacgca cgtgcatgat cttcaacata cagccagtcg cgaatttgat
    12781 cccctttacc ataaataggt aatgccttac cttccagagc attaagaatt actagtggaa
    12841 tcaatttttc cggaaagtga taaggaccgt agttattcga acagttagtc acaatggtcg
    12901 gtaaaccata ggtacgtttc cacgcacgga ctaaatgatc gctagatgct tttgatgcgg
    12961 aataagggct gcttggcgcg taagctgttg tctcagtaaa taagggtaat tcttctgtat
    13021 tatttacctc gtcaggatga ggcaaatcac catatacttc gtcagtagaa atatgatgaa
    13081 aacggaagct atttttcttg tcgctatcaa gagcagacca gtaattgcga gcggcttcca
    13141 aaaggacata agtaccaaca atattggttt caataaatgc cgcagggcct gtaattgaac
    13201 gatccacatg gctttcagca gccaggtgca tcactgcatc cggctgatgc tgagcaaaaa
    13261 tccgtgccat tgcagctgca tcgcaaacat ccgcatgttc aaaaacatag cgttcagaat
    13321 cagaaacatc agcaagtgat tccaggtttc cggcgtacgt taatttatcg acattaacaa
    13381 cactatcctg cgtattattt ataatgtgac gaactacagc agaaccaata aatcctgcgc
    13441 caccagtaac aagtattttc acttaattta ttccatatta cttcagagca tgctgtgaaa
    13501 taagcggctc tttgtttgat taatagaggt attaatgcac gctaccgccc ctggctttac
    13561 agctaccag
//

</pre>