LOCUS       AB812025               22017 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: K6b.
ACCESSION   AB812025
VERSION     AB812025.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 22017)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..22017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="K6b"
                     /db_xref="taxon:562"
                     /note="serogroup:O64"
     gene            <1..853
                     /gene="wcaM"
     CDS             <1..853
                     /gene="wcaM"
                     /codon_start=2
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ01106.1"
                     /translation="TNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCTNGKINWG
                     IGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIKARNITPD
                     FSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYFSIPQNFRVNNIQL
                     DNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKVMQESSVG
                     PALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHILNVEKINF
                     RLPERGE"
     gene            1016..1921
                     /gene="galF"
     CDS             1016..1921
                     /gene="galF"
                     /note="O064_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ01107.1"
                     /translation="MDEIMTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVT
                     IMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNE
                     TGRSQVLAKRMPGDLSEYSVIQTKEPLEREGKVSRIVEFIEKPDQPQTLDSDIMAVGR
                     YVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQA
                     FVKYGLRNLKEGAKFRKGIEKLLSE"
     CDS             2619..3122
                     /note="O064_02"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase of IS1"
                     /protein_id="BAQ01108.1"
                     /translation="MPGNRPHYGRWPQHDFTSLKKLRPQSVTSRIQPGSDVIVCAEMD
                     EQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDG
                     WPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHY
                     LNIKHYQ"
     CDS             complement(3230..4045)
                     /note="O064_03"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ01109.1"
                     /translation="MTDNNVAVIMSVYHSDDPQMFKDAINSILYQSISCTLLIYRDGI
                     LPEPLNVVLESYSENKNVYIIRNDVNQGLATGLNTLIDFAIKKGFKYIARMDSDDISR
                     PQRLKVQLNFLESNTDIDVLGTSCREFGASFALKEKHLPTSHDELKDFSITRCPFIHP
                     TVVFRRRVFDKGYRYPIHTTFTEDMALWFDLLNAGFKFSNINEVLLDYRLSENTVNRR
                     KGIKKALSEIKVRFHNMISLKQVSVWNVTMIFARIIFHLMPNFLVKLAYKNAR"
     CDS             complement(4094..4924)
                     /note="O064_04"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase of IS1"
                     /protein_id="BAQ01110.1"
                     /translation="MLMCDATGLSQRRACRLTGLSLSTCRYEAHRPAADAHLSGRITE
                     LALERRRFGYRRIWQLLRREGLHVNHKRVYRLYHLSGLGVKRRRRRKGLATERLPLLR
                     PAAPNLTWSMDFVMDALSTGRRIKCLTCVDDFTKECLTVTVAFGISGVQVTRILDSIA
                     LFRGYPATIRTDQGPEFTCRALDQWAFEHGVELRLIQPGKPTQNGFIESFNGRFRDEC
                     LNEHWFSDIVHARKIINDWRQDYNECRPHSTLNYQTPSEFAAGWRKGHSENEDSDVTN
                     "
     CDS             complement(4951..5214)
                     /note="O064_05"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase of IS1"
                     /protein_id="BAQ01111.1"
                     /translation="MKKRFSDEQIISILREAEAGVPAGELCRKHAISDATFYTWRKKY
                     GGMEVPEVKRLKSLEEENTRLKKLLAEAMLDKEALQVALGRKY"
     CDS             complement(5264..6142)
                     /note="O064_06"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ01112.1"
                     /translation="MSNVFLLQVHQDTDQLRTLISILSNDCDVFIHFDKKQCSKTLSD
                     WIDNNCFTYKVSLIKSRVKVYWAHESQIYATLNLIKEARSYSNYKFYTLLSGDCFPVK
                     PVSEYIQFLSDNPSKNFIKTIISDKYCYRILLYHFGLKSNYYRKYLIFRKMSLFLGYV
                     YSIFIKREQFVYGDIGKGSQWFTLNNDFISYLFDNNKIESYEEKIKNTNCIDEMFFQS
                     LLLNSPFKKSIVNNNLRYIKWDGRKSPGIINENDLELCSDVFFCRKVSFTLSPSLKKI
                     FLEKVFTRDTDLLPVD"
     CDS             complement(6152..7036)
                     /note="O064_07"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ01113.1"
                     /translation="MFSLVITTKDREFFLKRTLKSVLKSNKLPSDIIIVNDAGSSVDL
                     SDLDFGLISVTVINNKKSFGANYCRNLGIKTALNEVVFLLDDDDVVTPDSFLNRYRII
                     NKDPEIGLCFTGIKIVSSKSLDLVKRIVPPVQVENFYYSLLNDGNIIGSTSRVVLRKK
                     YFYKAGTFDESLSCLQDYDLWIRMAKVCKIANDKEASVIYTVHESGKQTSSKFKNYEN
                     TGLVVFDKYKEDLYEFRLDRVFKSKLYLRCALSASSSSLRYKLYYACKSFILKPSLKS
                     LALIFVPNFFLKKFFLFA"
     gene            complement(7043..8272)
                     /gene="wzy"
     CDS             complement(7043..8272)
                     /gene="wzy"
                     /note="O064_08"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="BAQ01114.1"
                     /translation="MDSGNRSSIKIIKSNFIISICFFYTLTIYIFDAFLADNMFKFMG
                     NTSLMLPIKLLGLVLSFICATLYTPKISRLELFILFILFIYVSSVFLGTSDAYEAFYF
                     WSNYIGYFAIYLVTKIYLEKYGYYKLTKSITFISFLVFLLSIYVDFGAYERAAFRSEV
                     GGTYICSQLSVRKCGISSNPNILATMLLPIIFEYCYMLYNRLHTKTTLFVFFLCLLLL
                     VLTGSKTVLFLSLLFLCVTFTKISLLRQKSTFVILVSFSVLFLLIATIFGDYFYEIIS
                     SRFLSGNALDSSGRYEIYLGIFDKLNQNIIFGTGLDLREDYSQYFPGWLRLSDSVYGN
                     ILVSSGMFGFLIFTLTMFCFLATVIKKDRVRFLFLLVLSIGFLFEDYLLRGFSFMFIM
                     SVTFYLISTNFKFGNTK"
     CDS             complement(8239..9369)
                     /note="O064_09"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ01115.1"
                     /translation="MNFVIVIDRLYELDGSNITIGGIQTYLVNLSEIIYEKYKVKPLI
                     YQSGDNDFHIICDKYEVFGIACKSMNIRKKIKKIYSHIKDKFSKHNTVVIWGSDQYSL
                     NAQGFKSINIQHGIGFDTEALDSGFKSKLKKYNLVPIYKLMQRINALNYARNGNKVVC
                     VDYNFKNWIKTFVYKNKERFVVIPNFADTPCLSEHYDSESINILIARRFVKRRGIDLA
                     VKVARQILNNKSNINFTFAGSGPEQYLVDELKKEYPNSVYQTSYKSEDSIHFHQQYDI
                     AIVPSIGSEGTSLSLLEAMSAKCLVIATDIGGMTNIILDGFNGRMIPPIEEEMIACLN
                     DVIDNFDEYQYMRDNGRNTVEKAFSKDIWKHKWIQVIDQVLR"
     gene            complement(9347..10630)
                     /gene="wzx"
     CDS             complement(9347..10630)
                     /gene="wzx"
                     /note="O064_10"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="BAQ01116.1"
                     /translation="MKSTYMKEIFLDSLFTFISNSISRLSFIILSIVTAKVLISEDFH
                     SFTIMMSTLNMIVSSIGFAVGITIVKSAVEYHNNKTKKKEREYFSFIKLFIIGSLVVF
                     IGVSFLYNLISELLFNGKVYFALAFLTLTILALYYTVSSYFLIGVKDFKSLSKYNLLI
                     GTVFILSLLMVMYTDLSYNSLYTITLLLASFYFTGILLCVRKYAQLIPGFNRIEFKLH
                     STDENYFKKITLPAFLSNLVYAFTVWLQIIIVGYLLNDRNMASEVATGLIWFNALAFI
                     PQTISTVILPRISSENKNKLKILKVGMLINVCSTCILISFLTLTKSQINELYSSQFEQ
                     LADLILIMSFASLPNACCKVTGQYFIASSKMNISLMFNSLWAIVYIFTNVILLKFGFG
                     VFSVGYALIISYSILFFSQYVFIRVSETNELCNSN"
     CDS             complement(10652..11776)
                     /note="O064_11"
                     /codon_start=1
                     /transl_table=11
                     /product="putative UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="BAQ01117.1"
                     /translation="MTKKKILTVFGTRPEAIKMAPLVHALAADDRFEAKCCVTAQHRE
                     MLDQVLELFEITPDYDLNLMKAGQTLNDVTARIVQEIKPVLQEFQPDVVLVHGDTATT
                     FAASLAAYYEQIEVGHVEAGLRTGNIYSPWPEEANRRLTGVLTKYHFSPTETSKENLL
                     RENVSPEDIFVTGNTVIDALLMVKHKIDTNQDLNATLVAQFPFLDESRKLILVTGHRR
                     ESFGGGFERICEALAKTAKMHPEAQILYPMHLNPNVREPVNRILAGIENVHLVEPQQY
                     LPFIYLMSRASIILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVEAGTVKLVGTDTNN
                     IVSQLKKLLTDTDAYNEMSRAHNPYGDGLSSERIIDILSK"
     CDS             11858..12346
                     /note="O064_12"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase of IS1"
                     /protein_id="BAQ01118.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEHILPWQNMTAVIEPF
                     YPKADNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTSENFILK
                     FD"
     CDS             12897..13400
                     /note="O064_13"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase of IS1"
                     /protein_id="BAQ01119.1"
                     /translation="MPGNRPHYGRWPQHDFTSLKKLRPQSVTSRIQPGSDVIVCAEMD
                     EQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDG
                     WPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHY
                     LNIKHYQ"
     CDS             13464..13967
                     /note="O064_14;
                     transposse of ISEc1"
                     /pseudogene="unitary"
                     /codon_start=1
                     /transl_table=11
     gene            14121..15527
                     /gene="gnd"
     CDS             14121..15527
                     /gene="gnd"
                     /note="O064_15"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ01120.1"
                     /translation="MSNQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            15776..16942
                     /gene="ugd"
     CDS             15776..16942
                     /gene="ugd"
                     /note="O064_16"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ01121.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAVELKDVADKVYTRDLFGSD"
     gene            17032..18066
                     /gene="wzz"
     CDS             17032..18066
                     /gene="wzz"
                     /note="O064_17"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ01122.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGS
                     AAPKVSEIQSMLIGRFSTTFSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGLSP
                     EAAQKQLAQYIQQVDDQVNEELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKELRIRQ
                     IQEALQYANQAQVTKPQIQQTQDVTQDTMFLLGSDALESMVKHEASRPLVFSSNYYQT
                     RQNLLDIDNLDVDELDIHAYRYVMKPTLPIRRDSPKRAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     CDS             complement(18259..18762)
                     /note="O064_18"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase of IS1"
                     /protein_id="BAQ01123.1"
                     /translation="MPGNCPHYGRWPQHDFTSLKKLRPQSVTSRIQPGSDVIVCAEMD
                     EQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDG
                     WPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHY
                     LNIKHYQ"
     CDS             19050..19922
                     /note="O064_19"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ01124.1"
                     /translation="MRDYKDYIYHGPNFYLPHKLERAVTTFHDISIFTCPEYHPKDRV
                     RYMEKSLHESLDSAKLILTVSDFSRSEIIRLFNYPAERIVTTKLACSSDYIPRSPVEC
                     LPVLQKYQLAWQAYALYIGTMEPRKNIRGLLHAYQLLPMEIRMRYPLILSGYRGWEDD
                     VLWQLVERGTREGWIRYLGYVPDEDLPYLYAAARVFVYPSFYEGFGLPILEAMSCGVP
                     VVCSNVTSLPEVVGDAGLVADPNDIDAISAQILQSLKDDSWREIATARGLAQAKQFSW
                     ENCATQTINAYKLL"
     CDS             20223..21047
                     /note="O064_20;
                     glycosyltransferase"
                     /pseudogene="unitary"
                     /codon_start=1
                     /transl_table=11
     gene            complement(21086..21694)
                     /gene="hisI"
     CDS             complement(21086..21694)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ01125.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNSEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNITPDCDNDTLLLLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQGLDLGEVIDNLKNRH"
ORIGIN      
        1 caccaactgt aaatttagtg atttacaggg ggacgctatt gaatggaacg tggcgattaa
       61 cgaccgtgat attttgatat ctgaccatgt catcgagcgc atcaactgta ccaacggcaa
      121 aatcaactgg ggcatcggca taggtcttgc gggaagcact tatgataata attacccgga
      181 agaccaggca gtgaaaaact ttgtcgtggc gaatatcacg ggatcggatt gtcggcagtt
      241 gatccatgtt gaaaatggta aacattttgt tattcgtaat atcaaagccc gcaatatcac
      301 gccggatttc agtaagaaag caggtattga taacgccacg gtcgctattt acggttgtga
      361 caatttcgtg attgataata ttgaaatgat taatagcgcc gggatgttaa tcggctatgg
      421 ggtaattaaa ggcaaatatt tctcgatacc gcaaaatttc cgagtgaata atattcaact
      481 ggataacact caccttgctt ataaattgcg cggcatccaa atctctgccg ggaatgctgt
      541 ctcctttgtg gcgctgacta acattgagat gaagcgtgcg tcgcttgagc tacacaataa
      601 accgcaacac ctttttatgc gtaatatcaa ggtgatgcag gaatcctctg ttggacccgc
      661 attgagcatg aacttcgaca tgcgcaaaga cgttcgcggc gtgtttatgg cgaaaaaaga
      721 aacactgctg tctcttgcaa atgttcatgc ggtgaatgaa agagggcaaa gctccgtcga
      781 tatcgacagg attaatcacc atattcttaa tgtggaaaag attaacttta gattgccgga
      841 acggggggag tagatttgcg accattcctg gaaaaatgga gccatactta ggaacaatgc
      901 tactgcaatc cacaacgaag cggcgtaaca tcacaagtaa ttcagtaatc aattcagggt
      961 aattgatgct ggcgaaaaaa atcgaacaag ctataattca gcaaccattt tacaggtgga
     1021 tgaaataatg acgaatttaa aagcagttat tcctgtagcg ggtcttggga tgcatatgtt
     1081 gcctgccact aaggcgattc ccaaagagat gctaccgatc gtcgacaagc caatgattca
     1141 gtacattgtt gacgagattg tggctgcagg gatcaaagaa atcctcctgg taactcacgc
     1201 gtccaagaac gcggtcgaaa accacttcga cacctcttat gaattagaat ctctccttga
     1261 acagcgcgtg aagcgtcagc tgctggcgga agtgcagtcc atctgtccgc cgggcgtgac
     1321 cattatgaac gtgcgtcagg gcgaaccttt aggtttgggc cactccattt tatgtgcacg
     1381 acccgccatt ggtgacaacc catttgtcgt ggtgctgcca gacgttgtga tcgacgacgc
     1441 cagcgccgac ccgctgcgct acaaccttgc tgccatgatt gcgcgcttca acgaaacggg
     1501 ccgcagccag gtgctggcaa aacgtatgcc gggtgacctc tctgaatact cagtcatcca
     1561 gaccaaagag ccgctggaac gcgaaggtaa agtcagccgc attgttgaat tcatcgaaaa
     1621 accggatcag ccacaaacgc tggattcaga catcatggcc gttggtcgct atgtgctttc
     1681 tgccgatatt tggccggaac ttgaacgcac tcagccaggt gcatgggggc gtattcagct
     1741 gactgatgcc atcgctgaac tggcgaaaaa acagtccgtt gatgccatgc tgatgacagg
     1801 tgacagctac gactgcggta aaaaaatggg ttatatgcag gcatttgtga agtatggact
     1861 acgcaacctg aaagaagggg cgaagttccg taaaggtatt gagaagctgt taagcgaata
     1921 atgaaaatct gaccgaatgt aacggttgat aagaaaatta taacggcagt gaagattcgt
     1981 ggtgaaagta atttgttgcg aattttcctg ccgttgtttt atataaacaa tcaggataac
     2041 aacgagttag caataggatt ttagtcaaag ttttccagga ttttccttgt ttccagggcg
     2101 gattggtaag acaattagcg tttgaatttt tcgggttaag cgcgagtggg taacgctcgc
     2161 tacatcgtag gcatgcatgc agtgctctgg tagctgtaaa gccaggggcg gtagcgtgca
     2221 ttaatacctc tattaatcaa actgagagcc gcttatttca cagcatgctc tgaagtaata
     2281 tggaataaat taagtgaaaa tacttgttac tggtggcgca ggatttattg gttctgctgt
     2341 agttcgtcac attataaata atactcaggg gtaatgctgc caacttactg atttagtgta
     2401 tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca
     2461 gctactgacg gggtggtgcg taacggcaaa agcactgccg gacatcagcg ctatctctgc
     2521 tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca acccggtacg
     2581 caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac cgcccgcatt
     2641 atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg cagtcggtaa
     2701 cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac gaacagtggg
     2761 gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac agtctccgga
     2821 agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg cgtcttatga
     2881 gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg ctgtatgaat
     2941 cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga attgagcggc
     3001 ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg tcgttctcaa
     3061 aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa cactatcaat
     3121 aagttggagt cattacccga atttcagtat ttgcttatta tctaaattcg cattaattca
     3181 aaagaacctg cacaaaataa agttaatagc aataaagtgg aaatataatt tatcgtgcat
     3241 tcttgtatgc caacttaact aaaaaatttg gcattaaatg aaatattata cgagcaaata
     3301 tcattgtaac attccaaaca ctaacttgtt tcaacgatat catgttatga aatctaactt
     3361 taatttcact taaagccttc ttgattcctt ttcgacgatt aactgtattt tcacttaaac
     3421 gatagtcaag caaaacctcg ttgatatttg aaaatttgaa acctgcgttt agcaggtcaa
     3481 accataatgc catatcttca gtaaacgttg tatgaattgg atacctgtaa cctttatcga
     3541 aaactctacg gcgaaaaact acggttggat gaataaaggg gcaacgagta atgctaaagt
     3601 cttttaattc atcatgacta gtaggcaagt gtttttcttt tagagcaaat gatgccccaa
     3661 actcgcggca agatgttcct aatacatcaa tgtctgtatt cgattctaaa aaatttaatt
     3721 gaactttcaa gcgttgaggc ctagatatgt catcactatc catacgagct atatatttga
     3781 atcctttttt aatagcaaaa tcaattaatg tatttagacc agtagcaaga ccttgattta
     3841 catcattacg gataatgtac acgtttttgt tctctgaata gctttcaaga acaacattta
     3901 aaggttcagg taatatccca tctctatata ttaacagagt acaggatata gactgataga
     3961 gtatactatt gatggcatct ttaaacattt gaggatcatc agaatgatat acactcatga
     4021 tgacggcaac attattatca gtcattaatc acctccgaag gtattgacct gcccccacga
     4081 ttagatacaa cactcagtta gtaacgtcgg aatcttcatt ctcagaatga ccctttctcc
     4141 agcccgctgc aaattcagac ggtgtctgat aattcagcgt ggagtgcggg cggcattcgt
     4201 tataatcctg ccgccagtca ttaataattt tcctggcatg aacgatatcg ctgaaccagt
     4261 gctcattcaa acattcatcg cgaaatcgtc cgttaaagct ctcaataaat ccgttctgcg
     4321 ttggcttgcc cggctggatt aagcgcaact caacaccatg ctcaaaggcc cattgatcca
     4381 gtgcacggca agtgaactcc ggcccctggt cagttcttat cgtcgccgga tagcctcgaa
     4441 acagtgcaat gctgtccaga atacgcgtga cctgaacgcc tgaaatccca aaggcaacag
     4501 tgaccgtcag gcattccttt gtgaaatcat cgacgcaggt aagacacttg atcctgcgac
     4561 cggtggaaag tgcgtccatg acgaaatcca tcgaccaggt cagattgggc gccgccggac
     4621 ggagcagcgg cagacgttct gttgccagcc ctttacgacg tcttctgcgt tttacgccca
     4681 gaccactgag gtgataaagc cggtacacgc gcttatgatt aacatgaagc ccttcacggc
     4741 gcagcaactg ccaaatacga cggtagccaa aacgcctgcg ctccagtgcc agctcagtga
     4801 tgcgccctga taaatgcgca tcagcagccg gacggtgagc ctcatagcgg caggtcgaca
     4861 gggataaacc tgtaagcctg caggcacgac gttgcgacag accggtcgca tcacacatca
     4921 acatcacggc ttcccgcttc tggtctgtcg tcagtacttt cgcccaagag ccacctgaag
     4981 cgcctcttta tccagcatgg cttcggcaag cagcttcttg agtctggtgt tctcttcctc
     5041 aagcgacttc aggcgcttaa cttcaggcac ctccataccg ccatacttct tacgccaggt
     5101 gtaaaacgtg gcatcggaaa tggcatgctt gcggcagagt tcaccggcgg gtaccccagc
     5161 ttcggcttcg cggagaatac tgatgatctg ttcgtcggaa aaacgcttct tcatggggat
     5221 gtcctcatgt ggcttatgaa gacattacta acatcggggt gtactaatca acggggagca
     5281 ggtcagtatc tcttgtgaag accttctcta aaaatatttt ctttagtgaa ggtgatagag
     5341 tgaaactaac ttttctgcag aaaaaaacat cactacataa ttcaagatcg ttctcattta
     5401 ttattccagg gcttttacgt ccgtcccatt taatgtatct aaggttgtta ttaacaatag
     5461 attttttaaa tggagagttt aacaacaaag attgaaaaaa catctcatca atacaatttg
     5521 tattttttat tttttcttca taggattcaa ttttgttgtt atcaaacagg tatgaaataa
     5581 aatcgttatt taaagtaaac cactgagatc cctttccaat atcaccatat acaaattgct
     5641 cacgttttat aaaaatcgaa tacacataac ctagaaataa actcattttt cggaatataa
     5701 ggtacttcct gtaataattt gactttaaac cgaaatgata taacaaaatc ctataacaat
     5761 atttatctga tataattgtt ttaataaaat ttttactagg attatcagaa agaaattgga
     5821 tatattcaga aacaggttta acagggaaac aatcaccgct gagtaacgta tagaatttat
     5881 agtttgaata acttctcgcc tcttttatta aattcagagt tgcatatatt tgtgactcat
     5941 gagcccaata aacttttact ctagatttaa tcaatgacac cttatatgtg aagcagttat
     6001 tatctatcca atctgataat gtcttactgc attgtttttt atcgaaatgt ataaaaacat
     6061 cgcagtcatt acttaaaatt gatattaaag ttcttaattg gtctgtatct tggtgaactt
     6121 gaagcaaaaa aacattggac ataaatactc cttatgcaaa taagaaaaat ttcttcaaaa
     6181 aaaagttcgg aacaaatatt aaagccaatg atttaagcga tggtttcagt ataaaagatt
     6241 tacaggcata atataattta tatcttagtg aactagatga agcagaaaga gcacatctta
     6301 aatatagctt agacttgaat accctatcta aacgaaattc atataaatct tctttatatt
     6361 tatcaaaaac aactaagccc gtattttcgt aatttttaaa ttttgaacta gtttgtttac
     6421 ctgattcgtg tactgtatat atgacgctag cttccttatc attagctatc ttacatacct
     6481 tagccattct tatccataaa tcataatctt gtaaacaact taatgactca tcaaaagtcc
     6541 cagctttata gaaatatttt tttctcaaga caactcgtga tgtagagcca ataatattgc
     6601 catcatttag taaactataa taaaaattct cgacttgtac tggtggtaca attcttttta
     6661 ctaaatcaag agattttgag ctcacaattt taatccctgt aaaacagagc cctatttctg
     6721 gatctttatt aattatacga tacctattca aaaatgagtc aggtgtaacg acatcatcat
     6781 catccaaaag aaaaacaact tcattaagag cagtttttat acctagattt ctacaatagt
     6841 ttgcaccaaa tgacttttta ttattaatta ctgttacaga tattaaccca aaatcaagat
     6901 ctgacaaatc tacagatgag cctgcatcat taacaataat aatgtcgcta ggtaatttat
     6961 tactttttaa tacacttttt aaagttcttt ttaaaaaaaa ctctctatcc tttgtcgtaa
     7021 taacaagtga aaacatataa ccctacttag tatttccaaa tttgaaattt gtcgatatca
     7081 gataaaatgt cactgacatt ataaacataa acgaaaagcc tctaagtaaa taatcttcaa
     7141 ataaaaaacc aatagacaat acaaggagga ataaaaacct aactctatct ttcttgataa
     7201 cagtagctaa gaaacaaaac atagttaaag taaatatcaa aaatccaaac atccctgaag
     7261 aaacaagaat atttccataa acagaatcac ttaatctaag ccaaccaggg aaatactgcg
     7321 aataatcttc acgcaaatcc aaacctgttc caaatataat attttgattt aacttatcaa
     7381 aaataccgag atatatttca tatcttccac tactatcaag ggcgttaccg gatagaaatc
     7441 ttgaacttat tatttcataa aaataatcac caaaaatagt ggctataagt aaaaataaaa
     7501 cagaaaatga aactaatatt acgaaagtac ttttttgcct taataaacta atttttgtaa
     7561 aagtaacaca taaaaacaat aaacttaaga atagtaccgt tttagagcct gtaagaacaa
     7621 gtaataataa gcacaaaaaa aatacaaaca aagttgtttt tgtatggaga cgattataga
     7681 gcatatagca atattcaaat ataattggta ataacattgt tgcaagtata tttggattac
     7741 ttgagatacc acactttctt acagataatt ggctacatat ataagttcct ccgacttcac
     7801 tacgaaatgc cgctcgttca taggctccaa agtctacata tatagagaga aggaaaacta
     7861 aaaatgaaat gaaggtgata gatttagtta acttataata cccatacttt tctaaataaa
     7921 ttttagttac taaatagatt gcaaaatagc caatatagtt actccaaaaa taaaaagctt
     7981 cataagcatc agaagttccc aaaaaaaccg atgatacata tataaacaga ataaaaagaa
     8041 taaataattc taaccttgaa attttaggag tgtacaaagt tgcacatata aaagataaaa
     8101 ccaatccgag taacttaata ggcagcatca gacttgtatt gcccataaac ttaaacatat
     8161 tatcagctaa gaaagcatcg aatatatata tagtcagcgt atagaaaaaa caaatagaaa
     8221 taatgaagtt agattttatt atcttaatac ttgatctatt acctgaatcc atttatgctt
     8281 ccatatatct ttactgaatg ctttttcaac agtatttctg ccattgtctc tcatgtattg
     8341 atattcgtca aaattatcta ttacgtcatt tagacaagct atcatttctt cctcaatagg
     8401 gggaatcatt cgtccattaa aaccatcaag aataatattg gtcataccac caatatcagt
     8461 tgcgattact aaacactttg ctgacatagc ttctaataat gatagtgaag taccctcgga
     8521 tccaattgaa gggactatcg ctatatcata ttgttggtga aaatggatac tatcttcaga
     8581 tttataactt gtctggtata cagagtttgg atattccttt tttagttcat caactaagta
     8641 ttgctctggg ccagaacctg caaaagtaaa attgatatta gacttattat ttaaaatttg
     8701 tctagcaacc ttaacagcta ggtctattcc tcgtcgttta acaaaacgtc gtgctattag
     8761 aatattaatg ctttcactat cataatgctc gctcaaacat ggagtatcag caaagttggg
     8821 aataacaaca aatctttctt tgtttttata aacaaaagtt tttatccagt ttttaaaatt
     8881 ataatcaacg catacaactt tattgccatt acgtgcataa tttaatgcat ttattctttg
     8941 cattaattta tatattggta ctaggttata tttttttagc ttacttttaa accctgaatc
     9001 taaagcttca gtatcaaaac caattccatg ttggatattt attgatttaa aaccttgagc
     9061 atttaagcta tattgatccg aaccccaaat aacaacagta ttgtgcttgc taaatttatc
     9121 ttttatatgt gagtatattt ttttaatttt tttcctaata ttcatactct tacatgcaat
     9181 accaaacact tcatatttgt cgcatattat gtgaaagtca ttatcgcctg attgatatat
     9241 tagaggctta actttgtatt tttcataaat aatctcagac aagttaacaa gataggtttg
     9301 aattccacca attgttatat tcgagccatc tagctcatat aaccgatcaa ttactattac
     9361 aaagttcatt tgtttctgaa acccttataa atacatactg gctaaaaaat aaaattgaat
     9421 aggatattat taaagcataa ccgacagaaa aaacaccaaa tccaaatttc aaaagaatta
     9481 cattagtaaa aatatatact attgcccata gtgaattaaa cattaatgaa atattcattt
     9541 ttgagcttgc aataaaatat tgcccggtta ctttacagca ggcatttggt aaagacgcaa
     9601 aactcataat taaaatcaaa tctgccaatt gttcaaactg gctagaatat agttcattta
     9661 tttgagattt agttaaagtt aagaatgaga ttaatataca agtagagcaa acattaatta
     9721 acatcccaac tttcaaaatc tttaatttat ttttattttc actagatatt ctaggtagga
     9781 ttacagttga aatagtttga ggaataaaag ccaatgcatt aaaccaaatc aaacctgtcg
     9841 caacttcact cgccatattc ctatcattta acaaataccc aacaattata atctgcaacc
     9901 aaacagtaaa tgcatatact aaattggata gaaatgcagg taaggttatt tttttaaagt
     9961 aattttcatc agtagagtgt aatttaaatt ctattctatt aaaaccagga attagttgtg
    10021 cgtattttct aacacacaat aaaattcctg tgaagtaaaa tgaagcaagt agtaatgtaa
    10081 tcgtgtataa tgaattatat gaaagatcgg tatacattac catcagcagc gagagaataa
    10141 aaacagttcc gataagtaaa ttatacttcg ataaactttt aaaatcttta acaccgatta
    10201 agaaatatga ggatactgta taataaagtg ctagtatagt aagagttaga aaagctaatg
    10261 caaaatatac ttttccatta aataatagtt cagatattag attatataaa aaacttactc
    10321 caataaaaac taccaaacta ccaatgataa atagttttat aaaactaaaa tattctctct
    10381 cttttttctt agtcttgtta ttatgatact caacagcaga tttgacaata gttataccaa
    10441 cagcaaatcc aattgatgat acgatcatat ttaatgtaga catcataatt gtgaaactat
    10501 gaaaatcttc tgaaattaga acttttgctg ttacaataga taatatgata aaagataatc
    10561 ttgagattga attacttata aaagtaaata aagaatctaa gaatatttct ttcatgtatg
    10621 ttgatttcaa atttactttc caatatctaa attatttact taaaatatca atgattctct
    10681 cagatgataa accatcaccg tatgggttgt gagcgcgact catttcatta taagcgtcag
    10741 tatcggttaa tagcttcttt aattgagaca caatgttgtt tgtatctgta ccaactaact
    10801 tcactgttcc agcttctact gcttccggtc tctctgttgt atctctcata actaacacag
    10861 gtttacctaa tgaaggcgct tcttcttgaa taccacctga atctgtcaaa ataatgcttg
    10921 cacggctcat tagatagata aatggtaagt actgttgtgg ttcaacaagg tgaacatttt
    10981 caatacctgc cagtattcgg ttcactggct cacgtacatt agggtttaag tgcattgggt
    11041 acaatatttg agcttctgga tgcatttttg ccgttttggc taacgcttca caaattcgct
    11101 caaatccacc accaaagctc tctctgcgat gcccagtaac caagattagt ttcctgcttt
    11161 catccaagaa cggaaattgt gctactaatg ttgcattcaa atcttgattg gtatcgatct
    11221 tatgtttcac cattaaaagt gcgtcgatga cggtattacc tgtcacaaaa atatcctctg
    11281 gtgaaacatt ttcgcgcaga aggttctctt ttgacgtttc cgtaggggag aaatgatact
    11341 tggtaagtac gccagttaaa cgtctattcg cttcttctgg ccatggggag tatatgttac
    11401 ccgtacgaag accagcttcc acatgaccta cctcgatttg ctcatagtac gcggctagac
    11461 tagcggcaaa agtcgtcgca gtgtctccat gtaccaaaac gacatctggc tgaaattctt
    11521 gaaggacagg ctttatctct tgtactattc gagctgtcac atcattaagc gtttgacccg
    11581 ctttcatcaa attcagatcg tagtccggcg tgatctcgaa tagctcgagt acctggtcta
    11641 gcatttctct gtgctgtgca gttacgcagc attttgcttc aaagcggtca tctgctgcta
    11701 atgcgtgaac aagaggggcc atttttatag cttctgggcg tgtgccaaaa accgtaagga
    11761 ttttcttttt agtcattaat atttcctagg gaaggtgcga acaagttcct gctatgagat
    11821 catcatattc atccggagcg catcccagag ggacatcatg agccatcaac tcaccttcgc
    11881 cgacagtgaa ttcagcacta agcgccgtca gacccgaaaa gagattttcc tctcccgcat
    11941 ggagcatatt ctgccatggc agaatatgac cgctgtcatc gagccgtttt atcccaaggc
    12001 ggacaatggc cgacggccct atccgctgga gaccatgctg cgtattcact gcatgcagca
    12061 ttggtacaac ctgagcgacg gtgccatgga agatgccctg tacgaaatcg cctccatgcg
    12121 cctgtttgcc cgattatccc tggatagcgc cctgccggat cggaccacca tcatgaattt
    12181 ccgccacctg ctcgagcagc atcaactggc ccgtcaattg ttcaagacca tcaatcgctg
    12241 gctggccgaa gcaggcgtca tgatgaccca aggcactttg gtggatgcca ccatcattga
    12301 ggcacccagc tctacctctg aaaattttat cctgaaattc gattaaaagg tgcgtcaata
    12361 acatgcgcgg accgtgatcc cttgaagaat ctgtttagcc aagctaccgc cctacgttgt
    12421 acacgcatga tcggagcgaa ttaatattct gtttttatgt ggcagagatt atgcacggat
    12481 ggtgtatgga tatcaaccac aaaagcggca tgaagtgctg aaattaatca acccatctat
    12541 caacaataat taacctgagc ttcggataag aatttagctc ccaaaaggaa ctaaatgttc
    12601 gttccctgat ccgatcattt gaccaagagt gattagaaaa aggaacgggt gatgctgcca
    12661 acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc tgtttctatc
    12721 agctgtccct cctgttcagc tactgacggg gtggtgcgta acggcaaaag caccgccgga
    12781 catcagcgct atctctgctc tcactgccgt aaaacatggc aactgcagtt cacttacacc
    12841 gcttctcaac ccggtacgca ccagaaaatc attgatatgg ccatgaatgg cgttggatgc
    12901 cgggcaaccg cccgcattat gggcgttggc ctcaacacga ttttacgtca cttaaaaaac
    12961 tcaggccgca gtcggtaacc tcgcgcatac agccgggcag tgacgtcatc gtctgcgcgg
    13021 aaatggacga acagtggggc tatgtcgggg ctaaatcgcg ccagcgctgg ctgttttacg
    13081 cgtatgacag tctccggaag acggttgttg cgcacgtatt cggtgaacgc actatggcga
    13141 cgctggggcg tcttatgagc ctgctgtcac cctttgacgt ggtgatatgg atgacggatg
    13201 gctggccgct gtatgaatcc cgcctgaagg gaaagctgca cgtaatcagc aagcgatata
    13261 cgcagcgaat tgagcggcat aacctgaatc tgaggcagca cctggcacgg ctgggacgga
    13321 agtcgctgtc gttctcaaaa tcggtggagc tgcatgacaa agtcatcggg cattatctga
    13381 acataaaaca ctatcaataa gttggagtca ttaccaaagg aaaccagggg cggctaaata
    13441 aagccttcga ggaaaaattt ccgttgaaag aattaagtaa tccagagcat gacagttacg
    13501 caattagtga aaagagtcac ggcagagaag aaatccgtct tcatattgtt tgcgatatcc
    13561 ctgatgaact tattgatttc acgtttgaat ggaaagggct gaagaaatta tgcatggcag
    13621 tctcctttcg gtcaataata gcagaacaaa agaaagagcc cgaaatgacg gtaagatatt
    13681 atatccgttc tgctcattta accgcagaga agttcgccac agcgatccga aatcactggc
    13741 acgtggagaa taagctgcac tggcgtctgg acgtggtaat gaatgaagac gactgcaaaa
    13801 taagaagagg aaatgcagca gaattatttt cagggatacg gcacatcgct attaatattt
    13861 taacgaatga taaggtattc aaggcagggt taagacgtaa gatgcgaaaa gcagcgatgg
    13921 acagaaacta cctggcgtca gtccttgcgg ggagcgggct ttcgtaatct tgccctgatg
    13981 atggggtata ggaagaagaa aagtattcgt tgcattaatg aaattcagca ctacacacat
    14041 tcgtgcaact tgagataaca tctcaatcat attcaagtcg cgcatacatc gcggtgaaca
    14101 ccccctgaca ggagtaaata atgtcaaatc aacagatcgg cgtcgtcggc atggcagtga
    14161 tggggcgcaa ccttgcgctc aacatcgaaa gccgtggtta taccgtctct attttcaacc
    14221 gttcccgtga gaaaacggaa gaagtgattg ccgaaaatcc aggcaagaaa ctggttcctt
    14281 actatacggt gaaagagttt gttgaatctc tggaaacgcc tcgtcgcatc ctgttaatgg
    14341 tgaaagcagg tgcaggcacg gatgctgcta ttgattccct caaaccatat ctcgataaag
    14401 gcgacatcat cattgatggt ggaaacacct tcttccagga caccattcgt cgtaatcgtg
    14461 agctttctgc cgaaggcttt aacttcattg gtaccggtgt ctccggtggt gaagaaggcg
    14521 cgctgaaagg tccttccatt atgcctggtg ggcagaaaga agcctatgaa ctggttgcgc
    14581 cgatcctgac caaaatcgcc gcagtggctg aagacggcga accgtgcgtt acctatattg
    14641 gtgccgatgg cgcaggtcac tatgtgaaga tggttcacaa cggtattgag tacggcgata
    14701 tgcagctgat tgctgaagcc tattctctgc ttaaaggtgg cctgaacctc accaacgaag
    14761 aactggcgca gacgtttacc gagtggaata acggtgaact gagcagttac ctgatcgaca
    14821 tcaccaaaga tatcttcacc aaaaaagatg aagacggtaa ctacctggtt gatgtgattc
    14881 tggatgaagc ggctaacaaa ggtaccggta aatggaccag ccagagcgcg ctggatctcg
    14941 gcgaaccgct gtcgctgatt accgagtctg tgtttgcacg ttatatctct tctctgaaag
    15001 atcagcgtgt tgccgcatct aaagttctct ctggcccgca agcacagcca gcaggcgaca
    15061 aggctgagtt catcgaaaaa gttcgccgtg cgctgtatct gggcaaaatc gtttcttacg
    15121 ctcagggctt ctctcagcta cgcgccgcgt cggaagagta caactgggat ctgaactatg
    15181 gcgaaatcgc gaagattttc cgtgctggct gcatcatccg tgcgcagttc ctgcagaaaa
    15241 tcaccgatgc ttatgccgaa aatccgcaga tcgctaacct gctgctggct ccgtacttca
    15301 agcaaattgc cgatgactac cagcaggcgc tgcgtgatgt cgttgcttat gcagtacaga
    15361 acggtatccc tgttcctacc ttcgctgctg cggttgccta ttacgacagc taccgcgccg
    15421 ctgttctgcc tgcgaacctg atccaggcac agcgtgacta tttcggtgcg catacttata
    15481 agcgcattga taaagaaggt gtgttccata ctgaatggct ggattaatct gatttaaatc
    15541 aattaatcaa tgcaaggccc ggagaaaccc tccgggcttt tttattatac aaagcggcag
    15601 gttatggcct ttttttataa tttatggtta aaaaagcgat ataatacaga gtcgcacagc
    15661 aggttcgctg tcttgacagt tcaaccgcat cagcgttaaa aatcccgcag tagatgaagt
    15721 tgtggtggtg gactaatgac cactctaaat gtttaaccgg aagaagtcag agctaatgaa
    15781 aataacaatt tcaggaacag gttatgttgg tctttcaaat ggtattctga ttgcgcaaaa
    15841 ccacgaagtg gttgcactgg atatcgttca ggccaaagtg gacatgctta acaagaagca
    15901 gtcaccgatt gttgataagg agattgaaga gtatctggcg actaaagatc tcaatttccg
    15961 cgctacgaca gataagtatg acgcgtataa aaatgccgat tacgttatta ttgccacgcc
    16021 taccgattat gatccgaaaa caaattattt taatacctca agcgtggaag cggtcattcg
    16081 cgatgtgaca gaaattaatc ctaatgctgt catggtgatt aaatctacaa ttccggtagg
    16141 tttcaccaaa tcaattaaag aacgtctggg tatcgataac cttattttct ctccggaatt
    16201 tctccgtgaa ggtaaagccc tttacgataa tctccatcct tcacgtattg tcattggtga
    16261 gcgttcagaa cgcgctgaac gtttcgctgc gttattacag gaaggcgcga ttaagcaaaa
    16321 tatcccaacc ctgtttaccg actccactga agcagaagcg attaaacttt tcgctaatac
    16381 ctatctagcg atgcgcgtag catactttaa tgaactggat agctatgcag aaagtttagg
    16441 tctgaatact cgccagatta tcgaaggcgt ttgtctcgat ccgcgtattg gcaaccatta
    16501 caacaatccg tcgtttggtt atggtggtta ttgtctgccg aaagatacca agcagttact
    16561 ggcgaactac cagtctgtgc cgaataacct gatttcggca attgtcgatg caaaccgcac
    16621 gcgtaaagat tttattgccg atgccatttt gtcacgcaaa ccgcaagtgg tgggtattta
    16681 tcgtctgatt atgaagagcg ggtcagataa cttccgtgcg tcttccattc aggggattat
    16741 gaaacgtatc aaggcgaaag gcgttgaagt gatcatctac gaaccggtga tgaaagaaga
    16801 ttcattcttc aactctcgcc tggaacgtga tctcgccacc ttcaaacaac aagccgacgt
    16861 cattatttcc aaccgtatgg cagtagagct taaggatgtg gctgacaaag tctacacccg
    16921 cgatcttttt ggcagcgact aacatcctgt tatcagggcg attttcgccc tgattctctt
    16981 atgttccctt tgtaataatt cattattttt atcatttatc ctatagcatt catggcgatt
    17041 atcgctaaac tatggcgact taaaattatt tcgtccgtta gggtaatgat gagagtagaa
    17101 aataataatg tttctgggca aaaccatgac ccggaacaga ttgatttgat tgatttactg
    17161 gtgcagttgt ggcgcggcaa gatgacaatt atcatctctg tcattgttgc tattgccctg
    17221 gctatagggt atctggccgt tgccaaagaa aagtggactt ccacagctat cgttactcaa
    17281 cctgatgtgg ggcaaatcgc tggttataac aacgccataa atgtgattta tggttcagct
    17341 gcgccgaaag tgtctgagat ccagtcgatg ttaattggtc gcttcagcac gacattttct
    17401 gcattagcag aaacgctgga taatcaggaa gaacctgaaa aactgaccat tgaacctact
    17461 gttaaaaatc agtcattacc tttagcggtg tcttatgttg ggctatcgcc tgaagctgcg
    17521 cagaaacagc tggctcaata tatccagcaa gttgacgacc aggtaaatga agaactggaa
    17581 aaagacctga aagacaacat cgcgctgcgt atgaaaaact tgcaagattc tctgaaaacc
    17641 caggaagtgg tcgcccagga gcagaaagag ctgcgtatcc gtcaaattca ggaagcgttg
    17701 cagtatgcga accaggcaca ggtgacaaag ccgcagattc agcagacaca ggacgtgacc
    17761 caggatacca tgttcctttt ggggagtgat gcactggagt cgatggttaa gcatgaagcg
    17821 agccgtccgt tggtgttctc gtcaaactac tatcagactc gtcaaaacct gctagatatc
    17881 gacaatcttg acgttgatga gcttgatatt cacgcttacc gctatgtgat gaaacctacg
    17941 ttacccatcc gccgcgatag tcctaaaagg gcaatcacct tgattctggc ggttctgctg
    18001 ggtggcatgg ttggggcggg tattgtactg gggcgtaatg cgctgcgtaa ttacaacgcg
    18061 aaatgatttt ttagcttaaa gaaagcgggc agggtggtcg cgtgcccgtt ttttttgttg
    18121 gatgcgatgc tggcgcacct tatccagctt acatgtgttg agaattttgt aagcgcaata
    18181 aacttgcgca ccgagcatta acggattagt tgttgccgga agtgactgta ataacacttc
    18241 cacggtaatg actccaactt attgatagtg ttttatgttc agataatgcc cgatgacttt
    18301 gtcatgcagc tccaccgatt ttgagaacga cagcgacttc cgtcccagcc gtgccaggtg
    18361 ctgcctcaga ttcaggttat gccgctcaat tcgctgcgta tatcgcttgc tgattacgtg
    18421 cagctttccc ttcaggcggg attcatacag cggccagcca tccgtcatcc atatcaccac
    18481 gtcaaagggt gacagcaggc tcataagacg ccccagcgtc gccatagtgc gttcaccgaa
    18541 tacgtgcgca acaaccgtct tccggagact gtcatacgcg taaaacagcc agcgctggcg
    18601 cgatttagcc ccgacatagc cccactgttc gtccatttcc gcgcagacga tgacgtcact
    18661 gcccggctgt atgcgcgagg ttaccgactg cggcctgagt tttttaagtg acgtaaaatc
    18721 gtgttgaggc caacgcccat aatgcgggca gttgcccggc atccaacgcc attcatggcc
    18781 atatcaatga ttttctggtg cgtaccgggt tgagaagcgg tgtaagtgaa ctgcagttgc
    18841 catgttttac ggcagtgaga gcagagatag cgctgatgtc cggcggtgct tttgccgtta
    18901 cgcaccaccc cgtcagtagc tgaacaggag ggacagctga tagaaacaga agccactgga
    18961 gcacctcaaa aacaccatca tacactaaat cagtaagttg gcagcatcac ccttatcgct
    19021 tgctgcatcc gcggcgccag gcgtgggcat tgcgcgacta taaagattat atctaccatg
    19081 gccccaattt ttatctgccg cataaactgg aacgcgccgt gaccacgttt catgacatat
    19141 ccatttttac ctgcccggaa tatcatccaa aagatcgggt tcgctatatg gagaagtccc
    19201 tacatgagag tctggattcg gcaaagctga tcctgactgt ttctgatttc tcgcgcagtg
    19261 aaattatccg cttgttcaac tatccggcgg agcggatcgt aaccaccaag ctagcctgca
    19321 gcagtgacta tatcccacgc agcccggtag agtgtctgcc ggtactgcag aaatatcagc
    19381 tggcgtggca ggcctatgcg ctatatatcg gcaccatgga gccacgtaaa aatatccgtg
    19441 gcctgctgca tgcctatcag ctgctaccga tggaaatccg catgcgctat ccgctaatcc
    19501 ttagcggcta tcgcggctgg gaagacgatg tgctgtggca gttagtcgag cgcggtactc
    19561 gggaaggctg gatccgttac ctcggatatg ttccggatga agacctgccg tatctatacg
    19621 cagcggccag agtctttgtt tatccctcct tctacgaggg attcggttta cctattcttg
    19681 aagcgatgtc ttgcggtgtg ccggtagtat gctccaatgt cacctctttg cctgaggttg
    19741 ttggcgatgc cggcctcgtt gccgatccta atgatataga cgcgattagc gcgcaaattt
    19801 tgcagagcct aaaggatgat agctggcggg aaatcgccac cgcgcgcggt cttgctcagg
    19861 cgaaacagtt ttcgtgggag aactgtgcga cccagaccat taacgcctat aaattactct
    19921 aagggtgtca gttgagagtt ctacacgtct ataagactta ctatcccgat acctacggcg
    19981 gtattgagca ggtaatttaa cagctaagtc agggctgcgc ccgccgggga atcgcagccg
    20041 atgttttcac ttttagcccg gacaaagata caggtcctgt cgcttacgaa gatcatcggg
    20101 tcatttataa taaacagctt tttgaaattg cctccacgcc gttttcgctg aaagcgttaa
    20161 atcgttttaa gctgattaaa gatgactacg atatcatcaa ctaccatttt ccgtttccct
    20221 ttatggatat gctgcatctt tcggcgcggc ctgacgccag gactgtggtg acctatcact
    20281 ctgatatagt gaaacaaaaa cggttaatga agctgtacca gccgctgcag gagcgatttc
    20341 tcagcggcgt agattgcatc gttgcctcgt cgcccaatta tgtggcttcc agccagaccc
    20401 tgaaaaaata tctggataaa acggtggtga tcccgtttgg tctggaacag caggacgtgc
    20461 agcacgatcc gcagagggtc gcgtactggc gggaaactgt cggcgataag ttctttctct
    20521 tcgtcggcac tttccgctac tacaaagggc tgcatattct gatggatgcc gctgagcgta
    20581 gccgactgcc agtggtggtt gtagggggcg ggccgctgga atcggaagtg cggcgtgaag
    20641 cgcagcagcg cgggctgagc aatgtgatgt ttaccggcat gctcaacgac gaagataagt
    20701 acattctctt ccagctctgc cggggcgtgg tattcccctc gcatctgcgc tctgaggcgt
    20761 ttggcattac gttattggaa ggcgcacgct tcgcaaggcc actgatctct tgtgagattg
    20821 gtacaggtac ctctttcatt aaccaggaca aagtgagtgg ttgcgtgatt ccgccgaatg
    20881 atagccaggc gctggtggag gcgatgaatg agctctggaa taacgaggaa acctccaacc
    20941 gctatggcga aaactcgcgt cgtcgttttg aagagatgtt tactgccgac catatgattg
    21001 acgcctatgt caatctctac actacattgc tggaaagcaa atcctgaaag attctacccc
    21061 cgccgttgct gcgggggcca acgcgttaat gccgattctt cagattatca atcacttctc
    21121 cgagatccag cccttgatcc tgcaacagca ccagcaggtg atacatcaaa tcagacgcct
    21181 cgttggtcag ctcaaagcgg tcatgtaccg ttgctgccag tgcggtttcc acgccttctt
    21241 cgcccacttt ttgcgcaatg cgtttggtgc cgctggcata cagtttggca gtgtaagagg
    21301 tttccgggtc ggcagatttg cgttcggcga gcaattgttc cagttgatac aggaacagcc
    21361 attggtgagc tgtgtcgccg aagcagctgc tggtgccttt gtggcaggtc gggccgatgg
    21421 gattcgccag caacagtaac gtgtcgttgt cgcagtccgg cgtaatattt actacgttga
    21481 ggaaattgcc cgacgtttcg cctttggtcc acagtcgctg tttagtgcgc gagaagaagg
    21541 tgactttgcc gctttcgatg gttttatcta aggcttccga gttcatatag cccagcatta
    21601 acacttcacc ggataccgcg tgttgcacaa tcaccggcat aagtccgtcg gttttttccc
    21661 agtccagttc gcgacgttgt tgttctgtta acatatcctg atctccacgc cctgtgttgc
    21721 caggtacgct tttaattcac caatattgat tatttgtttg tggaatacgg aagccgccag
    21781 cgcgccgtca acgtcggcat cgcggaaggc ttcgaggaag tgttccatgg tgcccgcgcc
    21841 accggaggca atcagcggga tgtggcaaac ctcacgcact tttttcagct gttcgaggtc
    21901 gtaaccgtta cgcacgccgt cctgattcat catattgagg acgatttctc cggcaccgcg
    21961 tttttgcact tcctgtaccc agtcgagcgt ttcccattgc gtgacgcggg tgcggct
//