<pre>LOCUS       EU549862               13492 bp    DNA     linear   BCT 10-DEC-2008
DEFINITION  Escherichia coli strain F10598-41 (O63:K-:H-) O-antigen gene
            cluster, partial sequence.
ACCESSION   EU549862
VERSION     EU549862.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13492)
  AUTHORS   Fratamico,P. and Yan,X.
  TITLE     Escherichia coli serogroup O63 O-antigen gene cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13492)
  AUTHORS   Yan,X. and Fratamico,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2008) Microbial Food Safety Research Unit, ERRC,
            USDA, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA
FEATURES             Location/Qualifiers
     source          1..13492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="F10598-41 (O63:K-:H-)"
                     /db_xref="taxon:562"
     misc_feature    <1..>13492
                     /note="O-antigen gene cluster"
     CDS             392..733
                     /codon_start=1
                     /transl_table=11
                     /product="H repeat-associated protein"
                     /protein_id="ACD75787.1"
                     /translation="MVILKMVFLFMKLLPELYPVSVLQNFTRALLTGCVIAILQMIKT
                     SSQLMEKRSGILMTRVAVGERFMSLVRSQQCTVWSSDRSRRIRNPMRSQLSLNFLTYW
                     ILKGKLSQLMR"
     CDS             1046..1360
                     /codon_start=1
                     /transl_table=11
                     /product="truncated H repeat-associated protein"
                     /protein_id="ACD75788.1"
                     /translation="MTVRYYISSADSTAEKFVTAIRNHWHMENNLYWRLDVVMNEDDY
                     KIRRGNAAESFSGVRHIAINILTNDQVFKARSRRKMRKATMDKNYLASVLAGAGFHNL
                     AL"
     CDS             2254..3207
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase-like protein"
                     /protein_id="ACD75789.1"
                     /translation="MQMNKWNITAISDIEPDVYLRPSFRISPFRSEDLVINLKKNDEE
                     LFYWDRFFDFERIGYYSNVMASGRECIDAVLSMLNLSREDKIYIATSSGNSYISGCVS
                     KTIEKYCSLTRVREKNIKAALVIHEFGKFCDNDEIEALKNDGVKIIEDYAHAFPVFFQ
                     NDTRAKGDYVVFSFPKFFQIHFGGLLLSKNEVNVQTKLKKESVNFIKVCINSFLPDLE
                     QIINSRKARYDYYSKELSRLRCKPYFHYDKCECPSVYLFYIDLNEYLLEKLKSFLQIR
                     GVECSVFYGEKAFFIPCNQRLCFSEIDMIIQLIESYIKGVK"
     CDS             3366..3686
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="ACD75790.1"
                     /translation="MNCCLNGYDSELIIGNNVSIASNVTIITGSGPNASLEMQKIYPI
                     VKESVEIGAHSWIGDNVTILPGVKLGAFCVVAANSVVTKSFDEYSVIGGTPAKLIKMI
                     QHKA"
     gene            3696..4586
                     /gene="wbuL"
     CDS             3696..4586
                     /gene="wbuL"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuL-like protein"
                     /protein_id="ACD75791.1"
                     /translation="MLDSISDYCYPECLFTSPKFYQLNADKCDKIHIIKTQDLAIVIG
                     LVDNKAYAPFSAPFTFLTSRKVNLKYSSYEAFFNQVKKDKELYGYTSLKITLPPDFYF
                     SDMHAKIRCCLAASGFNPLYRDINSHFNLSDFEIESLSNTAKKTIRASGKHNHEIFCA
                     KNDDEKKLVYDIIRRNRLSKGYPLRMTWEQVKKTVDNVARANFFYVKTNEKYSASAII
                     FDITDDISQVVYWGAVDEGETCKVMYFLPFELVKIYKSRGKKILDIGPCSEGGIVSQG
                     LNDFKQMIGCINSVKETWEI"
     gene            4574..5686
                     /gene="fdtB"
     CDS             4574..5686
                     /gene="fdtB"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminotransferase"
                     /protein_id="ACD75792.1"
                     /translation="MGNIMINFLNLKKINEQYKEELHNACLKVIDSGSYILGQYVNKF
                     EKEFAQYCGVKYCIGVANGLEALTLVLRAWKYMGLLEDKDEVLVPANTFIASILAITE
                     NNLVPVLIEPDDVTYNIDINKIIEKITSRTRVILPVHLYGQISDMRRISDIARKYNLL
                     VLEDCAQAHGASMGGQKSGSWGDAAGFSFYPGKNLGALGDAGAITTNSDSLYNVLLAL
                     RNYGSHRKYHHIYTGVNSRLDEIQAAMLCVKLKYLDSEIERRRSIAQFYCSHINNEKI
                     KLPVVDSKENAVWHLYVILTNERDALKQYLEQKGIQTLIHYPVPPHKQECYQDLFAEQ
                     VFPVSEKLHNSILSLPMDPTMTEGELHYIVDVINSF"
     gene            5742..7004
                     /gene="wzxB"
     CDS             5742..7004
                     /gene="wzxB"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ACD75793.1"
                     /translation="MQRFFKVISYTAFLTFIKMLCGIFISKAIAIYAGPSGMALFGQM
                     QGFTTMLTNLGNSPNNSGVIKYTAEFEHKGIDVYSQWWKAAIYWSFFVYITLFTLVVF
                     FSEEISIFLTGKNDYRWLVISAGMLIPLSALGVLCSSIYNSFRDYKNFFLSGMISTLI
                     TSVVIIYIILSYGLIGAMVAAVIQPALIGGGVFIFSLKEKWCRLKYFIGPITKGAKND
                     ILKYVAMISVSAIVGPLSIIILRTFLIEQVGWEIAGEWQSVWKISEVYLGVITLTLST
                     YYLPVLAKTRDYQQLVLELRRTVKVIFPLIVLMAIFIYILRDYIIYILFSKDFYNARE
                     LFLIQLLGDVIKIVSYIYAYPMLAKGKIKFFIFTEILFCITLITLSFFLINIFGVHGA
                     NIAYASNYLLYLIVVIMSFKRLYAQSCN"
     gene            6985..7635
                     /gene="wbuN"
     CDS             6985..7635
                     /gene="wbuN"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="ACD75794.1"
                     /translation="MRNPVIRKILFIDLCDTLYPENTTIGFIDNFYEEGAKLSRSRIV
                     KIISKLIFYLTKYDLLRYFYIRKLYGVPRVRLLKDAQDYIDKLIPNEKILKLIGEYKK
                     EGAELYLISASLDPIVEVVAKKFEFTSYFSSSLLYENNGLCLGKLHSDILGEKHTIIS
                     KFRKENHNIAFITDNYSDSVCIDLCDDFYAVIPKNKKTKFWENKNVKLIVLSDHEQ"
     CDS             7625..8500
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="ACD75795.1"
                     /translation="MNNKTLTIVIPSYNHKNYIRTSILNAMQLIDIADIVVIDDASDD
                     GTLDILTKESYPGVNIIIKKNNKGLLHSHRLWQQFVQTEYVYLIASDDEIIVDEFRKA
                     YNKLLALKDIDVAIFGGVNFLKEYEWPIYGKKHERFFMLSEKEKKIEIFTNHPSPLLA
                     QSTIFRTSVLSEIDAFDGKVKFDDYPIYIKLILNNSKYKLLFDNDVSIVRYRHHFDNT
                     YKKYNKMFDMFEEVYLYYCQDIKVQTESIALIWWLYIFRSLRDKNWNAFFNILSKGKL
                     SYMKYIYTFFIRRLP"
     gene            8500..9810
                     /gene="wzy"
     CDS             8500..9810
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="ACD75796.1"
                     /translation="MNYEDFFALAYNNIVEFFVISIFFLFFFVFTLRRFGAAGIIDPF
                     HFIFSFVFSTSYAVIIILYINNEIELFYLVLLTLYSISFLFPIYFFRNRRKDLVSKYI
                     YGFFSSINTSHYVTRIFILLYFIILIPLIYVKGFSLFTSTNRFEDNSGIGGLARFYDI
                     IWLFVAGSFILYYKRILLYGGTIRKTILFFPFLAFFILNMLVIGSKSELVQYVLFYYL
                     IISAYGYKFKLSIAKLTILGGLSLCFALIVLFFNFKMEGDGDFSNIINESFLRLSDRI
                     MSNGDMYYLGLPHNVIEKIKTNNVLIDFFVPVLSSKLISYLAGYDVYTYDIGKQILLF
                     HYPFYDIAGGPVEHFDLFGYKHFGIIGGIMFSAFLGMGVVILRNLVFLSRGNVFMTIV
                     TCSIYFKMLAVILKPSILFAFMFDFLSVYISVGVISILLVGKRS"
     gene            9812..10597
                     /gene="wbuO"
     CDS             9812..10597
                     /gene="wbuO"
                     /codon_start=1
                     /transl_table=11
                     /product="putative O-antigen acyltransferase"
                     /protein_id="ACD75797.1"
                     /translation="MLFFFVPFLYFYKTRLRSIIKLISWGVIYLVPIFLSTFSFANID
                     YYFISYFVVLTLTYNLYEIGYIQNDTETIKKEHNPTLRLSRDELFFYNNHRVYIYFVR
                     LFISFVLTIALAFFVYDKINAILVWSILLIFFFYNHVRSRLNLAIHFLLVVFRFVFPV
                     YVITSNVMLLFIMIFIFPVPNLIERTSEEKFGFLKLNKTIRPNISFFRVVYYFFLSLF
                     MGIICIIYNNHNELKTGFYISIFFFIYRLVILFSKFLAREKNA"
     gene            10618..11406
                     /gene="wbuP"
     CDS             10618..11406
                     /gene="wbuP"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ACD75798.1"
                     /translation="MCVYKNDKEQDIKLAIESIINQSIKNHLYIYIDGVVNNKVKDLL
                     GGYSSNRNIFIFESEINHGLAYGLNFLIDKILPCKYDYISRMDSDDISLPDRLFLQEK
                     FLDMNKSIDVIGGYCTEFGSEFALKVKKVPLEHNDIVNYSALRCPFIHPTVMFRSRVF
                     DSNIRYPVNTYYSEDLALWYILLANGYKFANIPEILLKYRITEDTLFRRKGFFKAKCE
                     AVLRWDYMKKMKIISPMKCTMLIIKMISQILPIFILKVLYKKYR"
     gene            11426..12502
                     /gene="rmlB"
     CDS             11426..12502
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6 dehydratase"
                     /protein_id="ACD75799.1"
                     /translation="MKIIVTGGAGFIGSALVKFIIDNTADHVLVLDKLTYAGNLLNLE
                     SISDNNRYQFINCDICDVVAVEKIFLDYKPDAIMHLAAESHVDRSISSSAVFIDTNII
                     GTYNLLNVSKEYWMQLSNEKKTQFRFHHISTDEVYGDLPHPDEFESISKLPLFTEDCA
                     YKPSSPYSASKASSDHLVNAWHRTYSLPTVITNCSNNYGPFQYPEKLIPMSIINALQG
                     EDIFIYGKGDQIRDWLYVYDHVEALYLVLTNGRVGQTYNIGGNNEKRNIEVVETICKI
                     LDEIKPKNGTYQDQIKFVNDRPGHDRRYAIDASKIKKELNWIPKESFYTGIRKTIKWY
                     LNNLDWVEKTRNGMLNNGNVRSPK"
     gene            12502..13380
                     /gene="rmlA"
     CDS             12502..13380
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="ACD75800.1"
                     /translation="MSMRKGIILAGGSGTRLYPVTMAVSKQLLPVFDKPMIYYPLSTL
                     MLAGIRDILIISTPHDLPRFINLLGDGSQWGISLDYKVQENPDGLAQAFIIAEDFLDG
                     NNCTLILGDNIFYGNDLQRQMEKADNKKNGATIFAYHVKDPERYGVVEFDSEWNAISL
                     EEKPKHPKSNYAVTGLYFYDNRVVDIAKSIKPSWRGELEITDINKEYLLSSELSVATM
                     GRGYAWLDTGTHESLIEASNFIQTIEERQGLKVACPEEIAFRKGFITAEKVLELAYPL
                     SKNSYGEYLINMVKEK"
ORIGIN      
        1 tgtgtgcggt agcacaaaaa tgcaaaaatg aacgtttgca tgtaataatc ataagtttat
       61 gaatagatca gggaaagaac aagtgacgtt gcttttttta ctatataatt cgcactgtat
      121 gttcaataat tgaaatattt cattttacat aaagctgttt ttcattgctt atacatgagt
      181 aaatactcct tacataatta aggaggacaa aatggaactt aaaaaattga tggagcatat
      241 ttctattatt cccgattaca gataagcttg gaaagtagag cataaattat cggatattct
      301 actgttggct atttttgcta ttatttctga tgcaaaggtt gggaaggtat agaggatttt
      361 tgggaaacac atcacgattt tttgaagcaa tatggtgatt ttgaaaatgg tattcctgtt
      421 catgaaacta ttgccagagt tgtatcctgt atcagtcctg caaaatttca cgagagcttt
      481 attaactgga tgcgtgattg ccattcttca gatgataaag acgtcatcgc aattgatgga
      541 aaaacgctcc ggcattctta tgacaagagt cgccgtaggg gagcgattca tgtcattagt
      601 gcgttctcaa caatgcacag tctggtcatc ggacagatca agacggataa gaaatccaat
      661 gagatcacag ctatccctga atttcttaac atactggata ttaaagggaa aattatcaca
      721 actgatgcga tgagttgcca gaaagatatt gcagagaaga tacaaaaaca gggaggtgat
      781 catttattcg ctgtaaaagg aaatcaggtg gcggcttaat aaagcctttg aggaaaaatt
      841 tccgctgaaa gaattaaata atccagagta tgacagttac gcaattagtg aaaagagtca
      901 ctgcagagaa gaagtccgtc ttaatattgt ttgcgatgtc cctgatggac ttattgactt
      961 cacgtttgaa tggaaaggac tgaaaaaatt gtgcgtggta gtctcctttc ggtcaataat
     1021 agcagaacaa aagaaaaagc cagaaatgac ggtcagatat tatatcagtt ctgctgattc
     1081 aaccgcagag aagttcgtca cagcgatccg aaatcactgg cacatggaga ataacctgta
     1141 ctggcgacta gacgtggtaa tgaatgaaga cgactacaaa atcagaagag gaaatgcagc
     1201 agaatcattt tcaggagtac gacacatcgc tattaatatt ttgacgaatg atcaggtatt
     1261 caaggcaagg tcaagacgta agatgagaaa agcaaccatg gataaaaact atctggcgtc
     1321 agtccttgcg ggagcgggct ttcacaatct tgccctgtaa catggcgcca ttacttattg
     1381 ttaacaaaga gaaatcagga agggttgaaa aacttttttg taaaatttac tgctgaaagt
     1441 attagataat ccttctcatg gcagttacgc aaaacgaaaa gaattacggt aaacatgaag
     1501 gcgtttattg tttacaatat ctctgataaa tttattgttt tcacatttta actgaaaggg
     1561 caggagaaaa tatatgtggt cttattctga acagggtaag aaatagaatt actgctactt
     1621 ggatgcagta ttgaatgaac atgatggtaa aattaaaagg ggaatgcaat ttttggagca
     1681 tattgctatt aatgttttga cgatagataa gatattcaag atgagttaag gcgtaagata
     1741 agaaaagtca ttatgtagag atgctaccta gtatcagtcc ttgaggcgga gagagagttt
     1801 cgttattttg acttgataat atccagagat ttttaatgag gtgtctgtga aaactgataa
     1861 ctataagtgt gtagaattca atgttataga gaatgactca gggagcttaa tatcaataga
     1921 aagagaatat ttgccattta tagttaaacg agcttattat atttataatg taaaagaaaa
     1981 taaagcaaga ggttttcatg cacataaaaa tttagaacag ttattaattt gtgtcaatgg
     2041 ttcatgtgaa atatctgtat gcgatggtaa agaaaaaaaa ctaattaagt tagacaaccc
     2101 atatgttggc ctgtatttga aagggctcgt gtggagggag atgtataact tcacaagaga
     2161 cgctgtatta ttagtgttgg caagtgatgt ttatgacgag cttgattata tcagagatta
     2221 tagtgagttt atgacaattg taaataaaga gacatgcaga tgaataagtg gaatataact
     2281 gccatttctg atattgaacc agatgtatat ttaaggccat cattccgaat aagtcctttc
     2341 agaagtgagg atttggtaat aaacctaaaa aaaaatgatg aggaattatt ttattgggat
     2401 cgtttttttg acttcgagag aattggatat tattcaaatg ttatggcctc agggcgagag
     2461 tgcattgatg ctgtattaag tatgttaaat ttatcaagag aagataaaat atatattgca
     2521 acatctagtg gaaattcata tataagtggc tgtgtatcaa aaacgataga aaagtattgc
     2581 tcgttgacca gagttagaga aaaaaatatt aaagctgcac ttgttataca tgaatttggt
     2641 aagttttgtg ataatgacga aatagaagct ttaaaaaatg atggtgtgaa aataattgaa
     2701 gactatgctc atgcttttcc tgtttttttt caaaatgata caagagctaa aggggattat
     2761 gttgttttta gttttccaaa attctttcag attcattttg gtgggttgtt gttatcaaaa
     2821 aatgaggtaa atgtacagac caagttaaaa aaagaaagtg ttaactttat aaaagtatgt
     2881 attaattcat ttttacctga tcttgaacaa attattaata gcagaaaggc ccggtatgat
     2941 tattatagta aagaattatc gagattgagg tgtaaaccat attttcatta tgataaatgc
     3001 gaatgtccaa gcgtgtattt gttttacatt gatttaaatg agtatctgtt agaaaagtta
     3061 aagtcttttc tacaaatcag aggggttgag tgcagtgttt tttatggtga aaaagcattt
     3121 tttattccat gtaatcaacg cttatgcttt agtgaaattg atatgatcat tcagttgatt
     3181 gaaagctata taaagggtgt gaagtgaaag ttcaaaatat atcaattaaa ggcagtctgg
     3241 atattgatga ctcttcatct gttaataatt gtgatctagg agatgggata aagatttcaa
     3301 aaagaagtgt tgtgtatggt tctaaagagc acccagtaag aatcggaaat ggttgttata
     3361 ttggcatgaa ttgttgctta aatggatatg attcagaatt aataatcgga aataatgtca
     3421 gtatagcatc taatgttacg attataacag gatcaggccc aaatgctagt ttagaaatgc
     3481 aaaagatata ccccattgtc aaagagagtg tggaaatagg tgcccattct tggattggtg
     3541 ataatgttac aattttgcct ggtgttaagt taggcgcctt ttgcgttgtt gctgcaaaca
     3601 gcgttgtaac aaaaagtttt gatgagtata gcgtcatagg tgggactccc gcaaaattaa
     3661 taaagatgat tcaacacaag gcgtaggaga tataaatgtt agatagtatt tctgattatt
     3721 gttatcctga gtgtttattt accagtccta aattctacca attaaatgct gataaatgtg
     3781 ataaaataca tataataaaa acacaagatt tagctatcgt aatcggttta gtagataata
     3841 aagcttatgc accttttagt gcgccattta cctttttgac atcgaggaaa gtgaatttaa
     3901 agtattcatc atatgaggct ttttttaatc aagtaaagaa agataaagaa ctatatgggt
     3961 atacatcttt aaaaattaca cttccaccgg atttttattt ttctgatatg catgccaaaa
     4021 tcaggtgctg tttggctgcg tctggattta atcctttata tagagatata aactctcatt
     4081 tcaatctgag tgattttgag attgaatctc ttagcaatac agccaaaaag acaataagag
     4141 catcaggaaa acataatcat gaaatatttt gtgcgaaaaa tgatgatgag aaaaaattgg
     4201 tttatgacat tatccgtaga aatagattaa gtaaaggata tccacttcgg atgacttggg
     4261 aacaagttaa gaaaaccgta gacaatgtgg ctagagctaa ttttttctat gttaaaacta
     4321 atgagaagta tagtgcttca gcaataatat ttgacattac agatgatatt tcccaagttg
     4381 tttattgggg ggctgtagat gaaggggaaa cttgcaaagt aatgtatttt ctaccatttg
     4441 agttagttaa aatatataag agtcgtggaa agaaaatttt agatataggt ccttgtagtg
     4501 agggtggtat agttagccaa gggcttaatg actttaaaca aatgatcggc tgtataaact
     4561 cagtaaaaga aacatgggaa atataatgat taacttctta aatctaaaaa aaataaatga
     4621 gcaatataaa gaagagctcc ataacgcctg tttaaaagtt attgactccg ggagttatat
     4681 tcttgggcaa tatgtaaata aatttgaaaa ggagtttgca caatattgtg gggtcaagta
     4741 ttgtataggt gtagcaaatg gattagaagc tttaacgtta gtactccgcg catggaagta
     4801 tatggggctt ttagaagata aggatgaagt tcttgttcct gcaaatactt ttattgcttc
     4861 aatattagca attaccgaga ataatttggt tccagtatta attgagcctg atgatgtaac
     4921 ttataatatt gacataaata aaattatcga aaaaataaca tcacgtacaa gagtcatttt
     4981 acctgtccat ttatatggac agattagtga tatgcggagg atatctgata tagcaaggaa
     5041 atataatctt ttggtccttg aagattgtgc tcaagcccat ggtgcaagta tgggggggca
     5101 aaagagtggc tcatggggag atgctgctgg ctttagcttc tatccaggga aaaatttagg
     5161 tgctttaggc gatgctggcg ctataacgac aaatagtgat tcattgtata atgtgttatt
     5221 ggcattaaga aattatggtt ctcatagaaa ataccatcat atttatactg gtgttaatag
     5281 tcgactcgat gaaattcagg cggcaatgct ttgtgttaag ttgaaatatt tagattctga
     5341 aattgaacgt agaagaagta tcgcacaatt ttattgttct catattaata atgaaaaaat
     5401 aaaattacct gttgtcgatt ctaaagaaaa tgctgtatgg catttatatg ttattttgac
     5461 gaatgaaagg gatgctttaa aacaatattt agaacaaaaa ggtattcaaa cgttaattca
     5521 ttatccggta ccaccacata agcaagaatg ttatcaggat ttatttgctg aacaagtatt
     5581 tcctgtgtca gaaaagttgc ataatagtat actttcattg cctatggatc caacaatgac
     5641 tgagggtgag ttacattata ttgtggatgt gataaattct ttttaatata ttaataatag
     5701 tgccagagtt tttctggcct actaactatg attaatggaa gatgcaaaga tttttcaaag
     5761 ttatatcata tacagctttt ttaacattta taaaaatgtt atgcgggata tttatatcta
     5821 aagccattgc aatatatgcg gggccaagtg gaatggctct ttttgggcag atgcaaggat
     5881 ttactaccat gttaactaat cttgggaact cgccaaacaa tagtggggtt ataaagtata
     5941 ccgcagagtt tgaacataaa ggtatagatg tatatagcca atggtggaaa gcggctatat
     6001 actggtcttt ttttgtttat attactcttt ttacgttggt agtgtttttc tctgaagaaa
     6061 tatccatctt tttaactggt aaaaatgatt atcgatggct tgttatttct gcaggcatgt
     6121 tgattccatt atctgcgtta ggtgttttat gttcatccat ttataattca tttagagatt
     6181 ataagaattt tttcctctcc ggaatgatat caaccttaat tacaagtgtc gtaataattt
     6241 atattatatt gagttatgga ttgattggcg caatggtggc tgctgtaata cagccagccc
     6301 tgattggggg aggagtcttc attttttcat taaaagaaaa atggtgtcga ttaaaatatt
     6361 ttatcggccc aattaccaag ggcgctaaaa acgatatttt aaagtatgta gcaatgatat
     6421 ctgtttcagc aatcgttgga cctttatcaa ttattatatt aagaactttt ttaattgaac
     6481 aagtaggatg ggaaatagca ggtgaatggc aatcagtatg gaagatatca gaggtatatt
     6541 taggcgtaat aactttaaca ttatcgactt attatctgcc tgtcttagcg aaaactcgag
     6601 attatcagca attagttctt gaattaaggc ggacagttaa agttatcttt cctctaatag
     6661 tattgatggc aatttttata tatattcttc gtgactatat aatatatata ttgttttcaa
     6721 aggatttcta taatgcacgt gaactatttc ttattcaatt gcttggtgat gttattaaaa
     6781 tagtcagtta tatatatgct tatcctatgt tagcgaaagg taaaattaaa tttttcatat
     6841 ttactgaaat tctcttttgc atcactttga ttactttatc attttttctt ataaatattt
     6901 ttggagtgca cggtgctaat attgcttatg catcaaacta tttattatat ttaattgttg
     6961 tgattatgag ctttaagagg ttatatgcgc aatcctgtaa ttagaaaaat cctgttcatc
     7021 gacctttgtg atacattgta tcccgaaaac actactatcg gttttataga taatttttat
     7081 gaggaggggg caaaactctc cagaagtcgt atcgtaaaga taatatctaa attgatcttt
     7141 tatctaacta aatatgattt gttgcgatat ttctatatta gaaagcttta tggagttccc
     7201 agagtaagat tattaaaaga tgctcaagat tatattgaca aattaattcc gaatgaaaaa
     7261 atattaaaat taattgggga gtataaaaaa gaaggagctg aattgtattt gatttcagcg
     7321 tcattagatc caatagtaga agttgttgct aagaagtttg agtttacatc ttatttttca
     7381 tctagtttgt tatatgaaaa taatggactg tgtttgggga aattacattc cgatatctta
     7441 ggtgaaaaac acacaataat tagtaagttc aggaaagaaa atcataatat tgcttttatt
     7501 acagataatt atagtgattc tgtatgtata gatttatgcg atgattttta tgcagttata
     7561 cccaaaaata aaaaaacaaa attttgggaa aataaaaacg ttaaattgat agtgctaagt
     7621 gatcatgaac aataaaacat taacaattgt gattccatct tataatcata agaattacat
     7681 tagaacctcg atattaaatg cgatgcaatt aatagatata gctgatattg ttgtaatcga
     7741 tgatgcatca gatgatggaa cactcgatat actaactaag gaaagttatc ctggtgtaaa
     7801 cataattata aaaaagaata ataaaggatt actccattcc catcggttat ggcagcagtt
     7861 tgtgcagact gaatatgttt accttatcgc gtctgatgat gaaattattg ttgacgagtt
     7921 ccggaaagct tataataaat tattagcatt gaaagatatc gatgttgcta tatttggtgg
     7981 tgttaatttt ttaaaggagt atgagtggcc aatatacgga aaaaaacatg aaagattttt
     8041 tatgttaagt gagaaagaaa aaaagattga aattttcaca aaccatccat ctcctttatt
     8101 ggctcaatcc actatttttc gaacaagtgt gctttctgaa attgatgcat ttgacgggaa
     8161 ggtaaaattt gatgattacc ctatttatat aaaactcatt cttaataatt caaagtataa
     8221 attgttattt gataatgatg tatcaatagt aagatataga catcattttg acaatacata
     8281 taaaaaatac aataaaatgt tcgatatgtt cgaagaggta tatttgtatt attgtcaaga
     8341 catcaaagtt caaactgaat ctattgcgtt aatttggtgg ttatatattt tccgttctct
     8401 gagagataaa aactggaatg cattttttaa tatattgtca aaaggcaaac tttcttatat
     8461 gaaatatatt tatacatttt ttattcggcg tttaccataa tgaattatga agattttttt
     8521 gctttggctt ataataatat agttgagttt tttgtcatat ctatattttt tttgtttttt
     8581 tttgttttta ccttgaggag attcggtgct gctggaatta tagatccttt ccattttatt
     8641 tttagttttg tattttcaac atcttatgct gtaattatta tattatatat aaataatgag
     8701 atagaattat tttatttagt tttgttgacg ctgtatagta tctcttttct ttttcccatt
     8761 tattttttta gaaacagaag aaaggatctc gtctctaaat atatatatgg ttttttttct
     8821 tctataaata ctagtcatta tgtgactagg atatttattc ttctgtactt tataattcta
     8881 atacctttaa tatatgtgaa aggtttttct ttattcacat cgacaaatcg gtttgaagac
     8941 aattcaggta taggtggatt agctcgtttt tatgatataa tatggttatt tgttgctgga
     9001 agttttattc tttactataa gcgtattcta ttatatggag gtacgataag gaaaacgatt
     9061 ttattttttc cattcttagc attttttatt ttaaatatgc tggtgattgg ctctaaatca
     9121 gagttggtac aatatgtgct tttttattac ttaataatat ccgcttatgg ttataaattt
     9181 aaattatcaa ttgctaaatt aacgatatta ggtggtttat cattatgttt tgcgctgatt
     9241 gttttgtttt ttaattttaa aatggaaggt gatggggatt tttcaaatat aattaatgag
     9301 tcttttttaa gactatcaga tcgcattatg tctaatggag atatgtatta tcttggtctg
     9361 cctcataatg tgattgaaaa aataaaaact aataatgtgt tgattgattt ttttgtacca
     9421 gtcttatctt cgaaactaat aagttatttg gcgggatatg atgtctatac ttatgatata
     9481 gggaagcaga tactattgtt tcattatcca ttttatgata ttgccggagg tcctgttgaa
     9541 cattttgacc tctttggata taagcatttc ggcatcatcg gtggcataat gttcagtgct
     9601 tttttaggta tgggagtagt catattaaga aatttagttt ttttatcaag aggcaatgtc
     9661 tttatgacaa tagttacgtg ctcaatttat tttaaaatgt tggctgtaat tctcaaacct
     9721 agtattttat ttgcatttat gtttgatttt ttaagtgtat acatttcagt tggtgtaata
     9781 tcaattcttt tggtaggtaa aaggagttag gatgctgttc ttttttgtac cattccttta
     9841 tttttacaaa actagattgc gtagcataat taaattaata tcttggggag tgatttattt
     9901 agttcctatt tttttgtcaa catttagttt tgcaaacatt gattattatt ttattagtta
     9961 ttttgttgtg ttgacgttaa catataatct atatgagatc ggatatatac aaaatgatac
    10021 ggaaacaata aagaaagagc ataatcccac cttgcgattg agtcgtgacg aattattttt
    10081 ttataataat catagggttt atatttattt tgttcgatta ttcatatctt ttgttttaac
    10141 aattgccctt gctttttttg tttatgataa aattaatgct atattagtat ggtcaatatt
    10201 attaattttc tttttttata atcacgtcag aagcagatta aaccttgcta ttcactttct
    10261 tttagttgta tttagatttg tgtttcctgt atatgtaatt acatctaatg taatgttgtt
    10321 gtttattatg atctttatat ttcctgtgcc taatttgatt gagagaactt ctgaggaaaa
    10381 atttggattt ttaaaattga ataaaactat aagacctaat atatcatttt ttcgagtggt
    10441 ttattatttt tttctttctc tctttatggg gataatatgc attatttaca ataatcacaa
    10501 tgaattgaaa actggttttt atatcagtat tttctttttt atttatcgac tggttatttt
    10561 attttcgaaa tttttagcac gggagaaaaa tgcgtgagta taagacatgc agtgattatg
    10621 tgtgtttata aaaatgataa agaacaagat ataaagttag caatagagag tatcataaat
    10681 caaagtataa aaaaccattt gtatatatat atagatggtg tagtaaataa taaagtgaaa
    10741 gatttattag gggggtattc ctctaataga aatattttta tttttgaatc tgaaattaat
    10801 catggattgg cttatggctt aaacttttta atagataaaa tacttccatg taaatatgat
    10861 tatatatcaa gaatggatag cgatgatatc tctttaccag acagattatt cttacaggaa
    10921 aaatttttag atatgaataa atctattgat gtaattgggg ggtattgtac ggagtttggc
    10981 tctgaattcg cgttgaaagt gaaaaaggta ccacttgaac ataatgatat agtaaactac
    11041 agcgcattga gatgtccatt tattcacccc acagttatgt ttcgctctcg tgtatttgat
    11101 tcaaatatca ggtatcctgt aaatacatat tattctgaag atttggcatt gtggtatata
    11161 ttgctggcaa atggatataa atttgcaaat atccccgaga ttttgttaaa atatagaata
    11221 acagaggata ctctttttag acgaaaaggt ttttttaaag caaaatgtga agctgtttta
    11281 aggtgggatt atatgaaaaa aatgaaaatc atttcaccaa tgaagtgtac tatgttaatt
    11341 attaaaatga tatcacaaat attgccaata tttatattga aagttttgta caaaaaatat
    11401 cggtgacttt tttaaagagg tgaacatgaa gatcattgtt actggtggtg ctggatttat
    11461 tgggtctgcc ttagttaaat ttattatcga taatacggct gatcatgttc ttgtattaga
    11521 taaattgact tatgcaggaa atttacttaa ccttgaatct atctctgata ataatcgata
    11581 tcaatttata aattgtgata tttgtgatgt tgttgcagta gagaaaatat ttttagatta
    11641 caaacccgat gcaataatgc atttggctgc ggagagtcac gttgatcgat caatatcttc
    11701 ttcagctgtg tttatcgata ccaatattat tggaacatat aacttactaa atgtttcgaa
    11761 agaatattgg atgcaacttt cgaatgaaaa aaaaactcag tttaggtttc atcatatatc
    11821 tactgatgag gtttatgggg atttacctca tcccgacgaa tttgaatcca tatctaagct
    11881 gcctctcttt actgaagatt gtgcatacaa gccaagtagt ccttattctg cctcaaaggc
    11941 atctagcgat catttagtta atgcctggca tcgaacgtat tcgctaccaa cggttataac
    12001 caattgttct aataattatg gcccctttca atacccggag aaactaattc cgatgtcaat
    12061 aataaatgct cttcagggag aagatatttt tatttatgga aagggagatc aaatacgaga
    12121 ctggttgtat gtatatgatc atgtcgaagc tttatattta gtattaacaa atggacgagt
    12181 tggccaaaca tataacattg gtggtaataa cgaaaaaaga aatattgagg ttgttgagac
    12241 aatttgcaaa attttagatg aaatcaaacc taaaaacgga acttatcagg atcaaattaa
    12301 atttgttaat gatcgtcctg gacatgacag aagatatgcg attgatgctt ctaaaataaa
    12361 aaaagagtta aattggatac ctaaggaaag tttttataca ggaataagaa aaacaataaa
    12421 atggtattta aataatctgg actgggtaga gaaaactcgt aatggcatgt taaataacgg
    12481 aaatgtaagg agccctaaat aatgtcaatg agaaaaggaa ttattttggc aggtggctca
    12541 ggaactagat tgtatcctgt aacaatggct gtaagtaaac agttgcttcc tgtatttgat
    12601 aagccaatga tttattatcc tctaagtaca ttgatgttag ctggtattag agatatattg
    12661 attataagta ctcctcatga tctccctaga tttattaacc ttcttggaga tggatcacaa
    12721 tggggaataa gtttagatta taaagttcaa gagaatccag atggacttgc tcaagcattt
    12781 attatcgctg aagattttct tgatggaaat aattgtactt tgattttagg cgacaatatt
    12841 ttttatggta acgacttaca gcggcaaatg gaaaaagctg ataataaaaa aaatggagct
    12901 acaatatttg cttatcacgt aaaagatccc gaacgttatg gtgttgttga gtttgatagt
    12961 gaatggaatg ctatttcatt agaggaaaaa cctaagcatc ctaaaagtaa ttatgctgtt
    13021 acagggctat atttttatga taatagagtt gttgatattg caaaatctat taaaccatcc
    13081 tggagaggtg agcttgaaat aacagacata aataaggagt atttattatc ctcagagctc
    13141 tctgtggcta cgatggggcg tggttatgct tggttagaca ccggtactca tgaaagcctt
    13201 attgaggcta gtaactttat tcaaacgatt gaggaacgac agggattaaa agttgcatgt
    13261 ccggaggaaa ttgctttcag gaaaggcttt attaccgcag aaaaggtatt ggaacttgcg
    13321 tatccgttgt ctaaaaattc atacggagaa tatcttatta atatggtgaa agagaaatga
    13381 attttgtaat atggttttat tttgacaaat aactgatttc ttttacgggt aataatataa
    13441 atccagtcgg agtaaacaat gtcaaagcaa cagatcggcg tcgtcggtat gg
//

</pre>