LOCUS       EU294175               17770 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Escherichia coli serogroup O58 O antigen gene cluster, complete
            sequence.
ACCESSION   EU294175
VERSION     EU294175.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17770)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 17770)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..17770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O58"
     misc_feature    1138..16268
                     /note="O antigen gene cluster"
     gene            1138..2223
                     /gene="rmlB"
     CDS             1138..2223
                     /gene="rmlB"
                     /note="dTDP-D-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ACA24873.1"
                     /translation="MKILVTGGAGFIGSTVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGSAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGDYQSWIEQNYEGRQ"
     gene            2223..3122
                     /gene="rmlD"
     CDS             2223..3122
                     /gene="rmlD"
                     /note="dTDP-4-dehydrorhamnose reductase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ACA24874.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDMPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            3180..4055
                     /gene="rmlA"
     CDS             3180..4055
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ACA24875.1"
                     /translation="MKTRKGTILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDRALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVAEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEKVKVLAEPL
                     KKNAYGQYLLKMIKG"
     gene            4063..4605
                     /gene="rmlC"
     CDS             4063..4605
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ACA24876.1"
                     /translation="MNVIKTEIPDVLIFEPKIFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSCKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFITLSNIAEFLYKTTDFYSPKHEVCIRYDDESIGIQWPKA
                     TDKMLISSKDKKGLDFTSLM"
     gene            4729..5916
                     /gene="wzx"
     CDS             4729..5916
                     /gene="wzx"
                     /note="O antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ACA24877.1"
                     /translation="MKINACLSFGTTLCKMITGLLFLAILSHSINNAEYSIIVTSIFF
                     AQLLSVFVDGGINNEVLSLTNKSLDNDIDGYQKLSMNGAVRLVVHLFFSFVLSTYFMF
                     ADGWSAGGVFFLGYISGVLTSILETYSIKLKTEYKYIEDFILNIFCSLFVILSSFFIY
                     IFPQCLMLYLVFFRLIPLAIYRKPLQVFKMIREIDYKLIRDNYIARKHYSIDSIATNI
                     NLQLDSFFLLLLFGRDMYAYYQPLNRLYNSCIGLSAAVVSFAIPYAHLLTSRIKKIYF
                     LVFLFSSSAIIISLSYYFFSRDVVMVFFGEKFSMERQYIFLFSLLIFMRYLCASFGSY
                     LMINGEQKKRALINLTITILCIPFILISETYSGILMVIIGGQILISYFYVLMIKRGVG
                     HDK"
     gene            5906..6514
                     /gene="wfeZ"
     CDS             5906..6514
                     /gene="wfeZ"
                     /note="acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeZ"
                     /protein_id="ACA24878.1"
                     /translation="MINKTHIIFLKFICRVLIKKNQFESCILRRLYIKLYDIDIGMYS
                     YGCFDPKRVPPKTKIGRYCSFAPTAYIFGRNHGVEFVSLHPYLYNSELGLVKQDTIAI
                     TSRVIEDDVWFGHNSVISPAVKFIGRGAVIATGAVVTSDVPRYAIVAGVPAKIIKYRF
                     EPRVINIIEKSKWWLRTKSELQKMIKLDSGFIFTPGYFDETN"
     gene            6501..7709
                     /gene="wffA"
     CDS             6501..7709
                     /gene="wffA"
                     /note="pyruvyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WffA"
                     /protein_id="ACA24879.1"
                     /translation="MKPIKICLAWHNINSTNYGVSALAVAQVALLVESASRSKVAIEL
                     ETFGTPFVNELSIRKEVEQRFAVKLTHRDFSLKKFIVDFAKLDFSGLRLFDKYDIVMD
                     IGEGDSFTDIYGIKRFITFSITKYLALRVKKKLILSPQTIGPFNSRISYIIARYLISK
                     CDSVFSRDYKSTKFIHDMGLNCEEVSDVAFTLPYDTLPKIDNSIGINISGLLWNGGYT
                     GDNQFGLTVDYKLFVKEIIELFRVRGKEVHLIGHVIADHLPVEDDYRVCNQIKEMFSL
                     DNGVIVAPKFTSPIQAKTYMSQLTFFTGSRMHATIGALSAGVVTVPIAYSRKFSGVFG
                     SLDYRFTLDAYTLDTKSLVTKLFEYYDNNFEEMQSAMINARIKANQRNEKYVGYLQEL
                     LSNEKNSTSH"
     gene            7684..8865
                     /gene="wffB"
     CDS             7684..8865
                     /gene="wffB"
                     /note="hydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="WffB"
                     /protein_id="ACA24880.1"
                     /translation="MKKIAQVIESGMCVGCGFCTENPNGMDINKEGYYRPISFIDDSL
                     SQLVCPGKSISHNNSMAPYNLLWGPVVSCESGNAVDPDIRHKGSSGGVLTAIAVYLVD
                     SGLVDAIIQVGVSVDNPIRNVTYIMKSQEDILKCAGSRYSPSSPLSVIRSLLGNGTRY
                     AVIGKPCDIAAMRTLVNSRQEFQEQFPYLLSFMCAGVPSEEGTRNILERWHIKHEHLI
                     SFRYRGDGWPGLTKAITDDGEEFTMTYNESWGGVLNRYLQPRCKLCADGIGEAADIVC
                     ADAWYSTTNGYPSFIEKEGRSLTIARTLKGRQLLDLALNKNVISLTPFNISDLEKIQP
                     YQANRKQTANVRRWAVMLLGGSVPNFKGYSLNKLMFRAPIKITLKAFFGTLIRKMKGR
                     I"
     gene            8978..9799
                     /gene="wffC"
     CDS             8978..9799
                     /gene="wffC"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WffC"
                     /protein_id="ACA24881.1"
                     /translation="MIKVNVIVALYYPNYYQRVREEIISFLQGFDYFIVFVDNNRTVK
                     PNIENNKNVTWIIGSNIGGEFSAWDEGYDLLVNMKNPSDDEIIVFINDTFCHHHFFTC
                     FDRYLYRKAISTCEDNNIYGDVNSIGEFFSVYDRHFSSWVSSYFFLGRKKNIDKLLPL
                     NKQSGMDAEYMRYLSNALVSKRVDVPIFSDKLNQHLTNWLFPVNSKGWYGARNVSQQL
                     ILFKLNAIINEKLLTYNIIEHDLLLTNIYHGKIRTLYNSMRNKLYFFCKNNKLIG"
     gene            9804..11135
                     /gene="wzy"
     CDS             9804..11135
                     /gene="wzy"
                     /note="O antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ACA24882.1"
                     /translation="MYANKAMPKRHTRERVFLPLLLSMIFICMIINDLPLQQYLGTLG
                     ASPMWGGALFIFMLIVIRSRFVLNLDSTSKYFLYFYLLTFTISLLQCFYYAISRGSVE
                     NEYGGLIFGKLVFASTYYIVYFFTIYTAIFLARRITSSALKFCIINASVLLLLLLILE
                     FFSPNSLSLFHKSMDGYGFGFRQRLLSPEPSMAAFTLNIFLLISIVLVNSKIAKLFMG
                     GALVVGNLFIGSKASLILILMSGVIVFYLNMNLTQKIKSLLILIPVSIAVFYVFLNTI
                     LPALIVDVDKFTSVSTRLITALAALLSLIYYPLGEGYGTYSSYLLGPLDLAVQLAEEL
                     LPFTLNVSEVNKMLMTGESLAAKSGILFSVIQSGVVSLIFFYVIYRNAFRKLCAARLS
                     NYNKIMMRLVLWYSLLSIMFAVNIEVVYAFLLPFIIIDHYAINYMRSYDYE"
     gene            11128..12252
                     /gene="wffD"
     CDS             11128..12252
                     /gene="wffD"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WffD"
                     /protein_id="ACA24883.1"
                     /translation="MNKILYISLSLDEKNYGGSIVSRNNLKALRALEDSEVKEVAIVK
                     KLKGIYEYELQTNVSKVKIAIDNLKGYAGRLNRDCLLKIKNIIKVFEPKFVYLDSSLL
                     GGIAELSKEISTDIKVITFFHNVEIDFELERLKSGGILYLPSLLPSYLAEKKAIKYSD
                     IIISLHKNDSARLKCIYGRSSDFCVPVCIEDDLSNEVILHKNNSTDSIFRVGFIGTAF
                     FANIKAAEFISKKLSPRFLNNKQIEFIIAGNGFDKYSKLLNRKNVKTLGYINSIEDFY
                     NEVDVIISPVLTGAGMKVKIAEAIKYNKKVIASSFSLIGYETMLNSQNIFSCITLDDY
                     AIAIKKLSTHPMTHSDTREYYLKFFSSQACINYFKYILNS"
     gene            12334..13443
                     /gene="wffE"
     CDS             12334..13443
                     /gene="wffE"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WffE"
                     /protein_id="ACA24884.1"
                     /translation="MKVLQVSKFYPPVHGGIEQVAFDISEGMFEEHIQAVDILCVDPL
                     GPRIDDRNYKYRVFRQKTFIVLFSTPISISFINKWRKIKDNYDIIHVHLPNPLAVLAI
                     YLFPPKGKIVLHWHSDIVKQKKLFRLFLPLQRWILDRCTYIIVTSPVYGQSSPSLQQY
                     QNKLICIPIGVDTSVMPVDLQEEKLIKSKYKDKKIIFSLGRLVYYKGMDILIDSAKYL
                     PDDYIILIGGGGALLKKLRRQIYDNNLSHKVVVLGSIDYKCLASYYKCCDVFCLPSIH
                     ESEAFGVVQLEAMSFSKPLVSTNIPRSGVPWVNENGTSGIVVEPRSAIALAEGIVKTI
                     ANSEKFSKGAKDRFDMLFTKRLMVNNVYKLYLSIK"
     gene            13452..14879
                     /gene="manC"
     CDS             13452..14879
                     /gene="manC"
                     /note="mannose-1-phosphate guanyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManC"
                     /protein_id="ACA24885.1"
                     /translation="MSLLPVIIAGGTGSRLWPLSRVKHPKQFLHLGDDGTMLQTTLNR
                     LQGLKCDNPIVICNEQHRFIVAEQLRQLNKLTQNIILEPVGRNTAPAVTLAALNAIRN
                     KSKQSKLILVLAADHIIKDEDAFCRSVLSAIPYANKGKLITFGIVPNSPETGYGYIKR
                     GHLCSGNNANLAFEVAEFVEKPNIDTAQEFLSSGNYYWNSGMFLFRADRYLDELKKYR
                     PDILEACKKSMIELNGDLDFIRINKDAFCACPDESIDYAVMEKTNDAVVIPMDAGWSD
                     VGSWSSLWEMSNKTIEENVIVGDVITYDSDSSYLRSDSGLLATVGVKDLVVVQTKDAV
                     LVANRNSVQNVKKIVERLKSENRSEVFTHLEVYRPWGKYESIDNGERYEVKRISVKPG
                     EGISLQMHHHRSEHWIIVSGTAKVTICDETRILSENESIYIPVGAKHCLENPGKIMLE
                     LIEVRSGSYLGEDDVIRFADRYGRT"
     gene            14880..16268
                     /gene="manB"
     CDS             14880..16268
                     /gene="manB"
                     /note="phosphomannomutase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManB"
                     /protein_id="ACA24886.1"
                     /translation="MHNKIILDKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKP
                     KTIVLGGDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVT
                     ASHNPMDYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDA
                     YVDHLFGYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGN
                     FPNGIPNPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLL
                     AEAFLEKNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGE
                     MSAHHYFRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAHP
                     VEAINRVEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLME
                     EKTKLILELLNK"
ORIGIN      
        1 cattgtggct gcagggatca aagaaatcct cctggtaact cacgcgtcca agaacgcggt
       61 cgaaaaccac ttcgacacct cttatgaatt agaatctctc cttgagcagc gcgtgaagcg
      121 tcaactgctt gcggaagtgc agtccatctg tccaccgggc gtgaccatta tgaacgtgcg
      181 tcagggcgaa cctttaggtt tgggccgctc cattttatgt gcacgacctg ccattggtga
      241 caacccattt gtcgtggtgc tgccagacgt tgtgatcgat gacgccagcg ccgacccgct
      301 gcgctacaac cttgctgcca tgattgcgcg cttcaacgaa acgggccgca gccaggtgct
      361 ggcaaaacgt atgccgggtg acctctctga atactctgtc atccagacca aagagccgct
      421 ggatcgtgag ggtaaagtca gccgcattgt tgaatttatt gaaaaaccag atcagccgca
      481 gacgctggac tcagacatca tggccgttgg tcgctatgtg ctttctgcag atatttggcc
      541 ggaacttgaa cgcactcagc ctggtgcatg ggggcgtatt cagctgactg atgctattgc
      601 cgaactggct aaaaaacagt ctgttgatgc aatgctgatg acaggcgaca gctacgactg
      661 cggtaaaaaa atgggctata tacaggcgtt tgtgaagtat ggactgcgta acctaaaaga
      721 aggggcgaag ttccggaaag ggattgagaa gctgttaagc gaataatgaa aatctgaccg
      781 gatgtaacgg ttgataagaa aattataacg gcagtgaaga ttcgtggcga aagtaatttg
      841 ttgcgaatct tcctgccgtt gttttatata aacaatcaga ataacaatga gttagcaata
      901 ggattttagt caaagttttc caggattttc cttgtttcca gagtggattg gtaagacaat
      961 tagcgtttga atttttcggg tttagcgcga gtaggtaatg ctcgtcacat cgtcggcatg
     1021 catgcagtgc tctggtagct gtaaagccag gggcggtagc gtgcattaat acctctatta
     1081 atcaaactga gagccgctta tttcacagca tgctctgaag taatatggaa taataaagtg
     1141 aagatacttg ttactggtgg cgcaggattt attggttcta ctgtagttcg tcacattata
     1201 aataatacgc aggatagtgt tgttaatgtc gataaattaa cgtacgccgg aaacctggaa
     1261 tcgcttgctg atgtttctga ttctgaacgc tatgtttttg aacatgcgga tatttgcgat
     1321 gcagctgcaa tggcacggat ttttgctcag catcagccgg atgcagtgat gcacctggct
     1381 gctgaaagcc atgtggatcg ttcaattaca ggctctgcgg catttattga aaccaatatt
     1441 gttggtactt atgtcctttt ggaagcggct cgcaattact ggtctgctct tgatggcgac
     1501 aagaaaaata gcttccgttt tcatcatatt tctactgacg aagtctatgg tgatttgcct
     1561 catcctgacg aagtaaataa taaagagcaa ttacccctct ttactgagac gacagcttac
     1621 gcgccaagta gcccatattc tgcttcgaaa gcatcaagcg atcatttagt ccgcgcttgg
     1681 aaacgtacct atggtttacc gaccattgtg actaattgtt cgaataacta cggtccttat
     1741 cactttccgg aaaaattgat tccactagta attcttaatg ctctggaagg taaggcatta
     1801 cctatttatg gcaaagggga tcaaattcgt gactggctgt atgttgaaga tcatgcgcgt
     1861 gcgttatata ccgtcgtaac cgaaggtaaa gcgggtgaaa cttataacat tggtggacac
     1921 aacgaaaaga aaaacatcga tgtagtgctc actatttgtg atttgttgga tgagattgta
     1981 ccgaaagaga aatcttaccg cgagcaaatt acttatgttg ccgatcgccc gggacacgat
     2041 cgccgttatg cgattgatgc agagaagatt agccgcgaat tgggctggaa accgcaggaa
     2101 acgtttgaga gcgggattcg taaaacggtg gaatggtacc tgtccaatac aaaatgggtt
     2161 gataatgtga aaagtggtga ctatcaatcg tggattgaac agaactatga gggccgccag
     2221 taatgaatat cctccttttt ggcaaaacag ggcaggtagg ttgggaacta cagcgtgctc
     2281 tggcacctct gggtaatttg attgctcttg atgttcactc cactgattac tgtggtgatt
     2341 ttagtaatcc tgaaggtgta gctgaaaccg taagaagcat tcggcctgat attattgtca
     2401 acgcagccgc tcacaccgca gtagacaaag cagaatcaga accggagttt gcacaattac
     2461 ttaacgcaac aagtgtcgaa gcgattgcga aagcagcaaa tgaagttgga gcttgggtta
     2521 tccattactc gactgattac gtcttccctg gaaatggcga catgccatgg ctggagacgg
     2581 atgcaaccgc accgctaaat gtttacggtg aaaccaagtt agctggagaa aaagcattac
     2641 aagagcattg tgcgaagcac ctaattttcc gtacaagctg ggtctatgca ggtaaaggaa
     2701 ataacttcgc caaaacgatg ttgcgtctgg caaaagagcg tgaagaatta gccgttatta
     2761 atgatcagtt tggtgcgcca acaggtgctg aactgctggc tgattgtacg gcacatgcaa
     2821 ttcgtgtggc actgaataaa ccagaagtcg caggcttgta tcatctggta gccagtggta
     2881 ccacaacctg gcacgattat gctgcgctgg tttttgaaga ggcgcgcaaa gcaggcattc
     2941 cccttgcact caacaagctc aatgcagtac caacaacagc ctatcctaca ccagcccgtc
     3001 gcccccataa ctctcgcctt aatacagaaa aatttcagca gaactttgcg cttgtcttgc
     3061 ctgactggca ggttggtgtg aaacgaatgc tcaacgaatt atttacgact acagcaattt
     3121 aatagttttt gcatcttgtt cgtgatggtg gagcaagatg aatcaaaagg aatgatgaaa
     3181 tgaaaacgcg taaaggtact attttagcgg gtggttctgg tactcgtctt tatcctgtga
     3241 ctatggctgt cagtaaacag ctattaccta tttatgataa gccgatgatc tattacccgc
     3301 tttctacact gatgttagcg ggtattcgcg atattctgat tatcagtacg ccgcaggata
     3361 ctcctcgttt tcaacaactg ctgggtgacg ggagccagtg ggggctaaat cttcagtata
     3421 aagtgcaacc gagtccagat ggtcttgcgc aggcatttat catcggtgaa gagtttatcg
     3481 gtggtgatga tcgtgctttg gttcttggtg ataatatctt ctacggtcat gatctgccga
     3541 agttaatgga tgtcgctgtt aacaaagaaa gtggtgcaac ggtatttgcc tatcacgtta
     3601 atgatcctga acgctacggt gtcgttgagt ttgataaaaa tggtacggcg atcagcctgg
     3661 aagaaaaacc gctacaacca aaaagtaatt atgcggtaac cgggctttat ttctatgata
     3721 acgacgttgc ggaaatggcg aaaaacctta agccttctgc ccgcggtgaa ctggaaatta
     3781 ccgatattaa ccgtatttat atggaacagg ggcgtttatc tgttgccatg atgggccgtg
     3841 gttatgcatg gctggacacg gggacacatc aaagcctgat tgaggcaagc aacttcattg
     3901 caacaattga agaacgtcag gggctgaaag tttcctgccc ggaagaaatt gcttaccgta
     3961 aagggtttat tgatgctgag aaggtgaaag tattagctga accgctgaaa aaaaatgctt
     4021 atggtcagta tctgctgaaa atgattaaag gttagtaata aaatgaacgt tattaaaaca
     4081 gaaattccag acgtattaat tttcgaaccg aaaatttttg gtgacgagcg cggtttcttt
     4141 ttcgagagct ttaaccagaa ggtttttgag gaagctgtag gccgcaaagt tgaatttgtt
     4201 caggataatc attcgaagtc ttgcaaaggt gttttacgcg gactgcatta tcagttagaa
     4261 ccttatgctc aaggaaaact ggtacgctgt gttgttggtg aagtttttga tgttgcggtt
     4321 gatattcgta aatcgtcacc tacctttggc aaatgggtag gggtgaattt atctgctgag
     4381 aataagcgcc agttatggat cccggagggg tttgcgcatg ggttcataac gttgtcaaat
     4441 atagccgaat ttctttataa aacaacagat ttttattctc caaaacatga ggtttgcatt
     4501 cggtatgatg atgagagcat tggtatacaa tggcctaaag caactgacaa gatgttgata
     4561 tcttcaaaag ataaaaaagg tttggatttt acttctttaa tgtgagttat tatatgctaa
     4621 ttttatatct attaatttct tcgcatgcat ctgaaaattt attatagctg gctcaatgga
     4681 cattacattg atttctatta atggatggtt ttactcaaag taaatataat gaaaataaat
     4741 gcttgtttgt catttgggac aacattatgt aagatgatca ctggtcttct ctttttggca
     4801 atcttatcgc attcaataaa taatgctgaa tatagcatta ttgtaacatc gatttttttt
     4861 gcccagttac tctccgtttt tgttgatggt gggatcaata atgaagttct ctcactaact
     4921 aataagtcat tagataacga tattgatgga tatcagaaac tatcaatgaa tggagcagtt
     4981 aggctagttg ttcatttgtt tttttctttt gtgttatcaa cttattttat gtttgctgat
     5041 ggatggagtg ctgggggggt gttttttttg ggatacattt ctggggtact aacctcaata
     5101 ttagaaacat actctataaa attaaaaaca gaatataaat atattgagga ttttatttta
     5161 aatatttttt gcagtctgtt tgttatttta tcatcttttt ttatttacat atttcctcaa
     5221 tgtcttatgt tatatcttgt tttttttaga ttaatccctt tggctatata tagaaaacca
     5281 ttgcaagtat ttaaaatgat aagagaaata gattataaat taataaggga taattatatc
     5341 gcacggaaac attactctat cgattcgatt gcgacaaaca ttaatttaca attagactcg
     5401 ttttttctat tgttattatt cggtagagat atgtatgcat attatcaacc attgaatcgg
     5461 ttatataatt catgtatagg gttgtcggca gcagttgtat catttgctat tccatatgca
     5521 catttactga caagtagaat aaagaagata tactttttag ttttcttgtt ttcatcatca
     5581 gcaattataa tttcattatc atattatttt ttttctagag atgtcgtgat ggtattcttt
     5641 ggtgaaaaat tttctatgga aagacaatat atatttctat ttagtttatt gatttttatg
     5701 cgatatttat gcgcctcatt tggttcatat ttaatgataa atggagagca aaaaaaaaga
     5761 gcactcataa atcttactat tactatttta tgcattccat ttatattaat ttctgaaacg
     5821 tattctggaa tattgatggt gataattgga gggcaaatat taatatcata cttttacgta
     5881 ctaatgatca aaagaggagt tggtcatgat aaataaaacc catatcattt ttctgaagtt
     5941 tatatgtcgt gttctcatta aaaaaaatca atttgaaagt tgtatattga gacgattata
     6001 tataaaacta tatgatattg atattggtat gtatagctat ggttgttttg accctaaaag
     6061 agtgccacct aaaacaaaaa taggaagata ttgttcgttt gcaccaacag catatatatt
     6121 tggacgaaat catggggttg aatttgtctc gttgcatcca tatttatata attcagagtt
     6181 gggtttagta aaacaagaca ccatagcaat aacttcacga gtgatagaag acgacgtttg
     6241 gtttgggcat aatagtgtaa tttcacctgc ggtaaaattt atagggagag gggctgttat
     6301 cgctactgga gcagttgtca ctagcgacgt tcctagatat gcgattgttg caggagttcc
     6361 ggcaaagatc atcaaatata gatttgaacc gagagtgata aatattattg aaaagagtaa
     6421 atggtggtta agaacgaaat ctgaattaca aaaaatgatt aaattagata gtgggtttat
     6481 atttacaccg gggtattttg atgaaaccaa ttaaaatttg cttagcatgg cacaatatta
     6541 attcgactaa ctatggcgtt agtgctcttg cagttgcaca agttgctctt ttagttgagt
     6601 ctgcgagtcg ttcaaaagtt gctattgaat tggagacttt tggtacaccg tttgttaatg
     6661 agttatcaat tagaaaggaa gtagagcaaa gatttgcagt taaattaaca catagagatt
     6721 tctcattaaa aaagtttatt gttgattttg caaaacttga tttttctgga cttagattat
     6781 ttgacaaata cgatattgta atggacattg gtgagggtga cagttttacg gatatatatg
     6841 gaattaagag atttattaca ttctcaatta caaagtattt agcgttaaga gttaaaaaga
     6901 aactcattct ttcacctcaa acaatcggac cttttaatag cagaatatca tatataatag
     6961 cgcgttattt gatttcgaaa tgtgattcag ttttttctcg tgactataaa tctacgaaat
     7021 tcatacatga catgggattg aattgtgaag aagtatcaga tgtagcattt actctaccat
     7081 atgatacttt gcccaagata gataacagta ttggtatcaa catttcaggt ttgttatgga
     7141 atggtggtta cacaggcgat aatcagtttg gattgacagt tgattataag ttattcgtca
     7201 aagagattat tgaattattt agagttagag ggaaggaagt tcatttgatt ggacatgtca
     7261 ttgcagacca tctacctgtg gaagatgact atcgtgtttg caatcaaatt aaagaaatgt
     7321 tttcattaga taatggagta atagtagcac ctaaatttac atcacctatc caagctaaaa
     7381 cttatatgag tcagttaaca ttttttactg gatcacgtat gcatgcaacc attggggcac
     7441 tatctgcagg agttgtaaca gtacctatag cgtatagtcg aaaattctct ggagtatttg
     7501 gtagtttgga ttatcgtttt actttggatg cttatacatt agataccaaa agtttagtga
     7561 caaaattatt tgagtattat gataataatt ttgaagaaat gcaatctgca atgattaatg
     7621 caagaattaa agcaaatcaa agaaacgaaa aatatgttgg ttatcttcag gaattattaa
     7681 gcaatgaaaa aaatagcaca agtcattgaa tctggaatgt gtgtgggttg tggattttgc
     7741 actgaaaacc ccaatggtat ggatattaat aaagaaggat attatagacc tatttctttc
     7801 attgatgatt cattatcaca gctagtatgc cctggtaaat ctatttccca taataacagt
     7861 atggctcctt ataacctttt atgggggccg gtagtgagtt gtgagagtgg aaatgcagtt
     7921 gacccagata tacgtcataa ggggtcttct ggtggggttt taaccgccat tgcagtttat
     7981 cttgttgatt ctggattggt tgatgcaata atacaggttg gtgtttcagt tgataatcca
     8041 atacgcaatg taacatatat aatgaagagt caggaagata tattaaaatg tgctggctct
     8101 cgctattcac cttcatcacc acttagtgtc attcgttcat tattaggtaa tggtacacgg
     8161 tacgctgtta ttggtaaacc ctgtgatatt gcggctatgc gcacattggt taatagtcgg
     8221 caagaatttc aagagcagtt tccataccta ctttcattta tgtgtgctgg agtgccaagt
     8281 gaagaaggta caagaaatat tcttgagcga tggcacataa aacatgaaca tttaatctcg
     8341 tttcgttatc gaggagacgg atggccagga ttgacaaaag ctattactga tgatggtgaa
     8401 gagtttacta tgacttataa tgaatcatgg ggcggtgtac ttaatcgcta cttacagcct
     8461 cgttgcaaac tatgtgctga cggtattggt gaagctgctg atattgtatg tgcagacgct
     8521 tggtattcta ctactaacgg ctatcctagt tttatagaga aggagggacg tagtttaaca
     8581 attgctcgta cacttaaagg ccgtcaattg ttggatcttg ctttaaataa aaatgtaata
     8641 tcacttacac cttttaatat ttctgatctt gagaaaattc aaccatacca agctaatcgt
     8701 aaacagacgg caaatgttcg tcgctgggct gttatgcttc tggggggaag tgtgccgaat
     8761 tttaaaggtt atagtcttaa taaattaatg tttagagccc caataaaaat tactttaaag
     8821 gcattttttg gtacattgat aagaaaaatg aagggacgta tttgaaaact aattatatat
     8881 tcttcggttt ttttgtgagc attgtacaat catcactgat tataaataac cgcaatagga
     8941 attattaaac gtgttgaata acaatatggt attttaaatg attaaagtaa atgttattgt
     9001 tgcactttat tatccaaatt actatcagag ggttagggaa gagattattt cttttctgca
     9061 gggatttgat tattttatag tgtttgtgga caataataga acggtcaaac caaatataga
     9121 gaataataaa aatgtaacgt ggataatagg gagtaatatt ggtggtgaat tttctgcttg
     9181 ggatgaaggt tatgacctcc tggttaatat gaaaaatccg tcagatgatg aaattatagt
     9241 ttttattaat gatacatttt gtcatcacca tttttttaca tgtttcgata ggtatttata
     9301 taggaaagca atatctacat gtgaagataa taacatttat ggtgatgtta acagtatagg
     9361 cgaatttttt agtgtttatg atcggcattt ttcatcttgg gtatcttctt atttttttct
     9421 aggtagaaaa aaaaacattg ataaactttt acctcttaat aaacaaagtg gtatggacgc
     9481 agaatatatg agatatctta gtaatgcatt ggtatctaag cgcgttgatg ttcctatctt
     9541 ttcggataaa ttgaatcagc atctcacgaa ttggttattt ccagttaata gcaagggatg
     9601 gtatggcgca agaaacgtat cacagcaact tattctgttt aaactgaatg caataattaa
     9661 tgaaaaattg ctcacttata atataattga acatgatctt ttattaacaa atatttatca
     9721 tggaaaaata agaacgcttt acaattctat gcgtaacaag ctatattttt tttgcaaaaa
     9781 taataagttg atagggtgat ataatgtatg caaataaagc aatgcccaaa agacatacga
     9841 gagaaagagt gtttttacca ctattacttt cgatgatttt catttgcatg ataataaatg
     9901 atttgccact acaacaatat cttggaactt taggtgcaag tcctatgtgg gggggggcat
     9961 tatttatttt tatgttgatt gttattcgta gccgctttgt tttaaatctt gattctacca
    10021 gtaaatattt tctctatttt tacctattaa catttactat atcgctttta caatgtttct
    10081 attatgccat ctctagaggt tctgtagaaa acgagtatgg tggattgatt tttggtaagc
    10141 ttgtatttgc ttcgacgtat tatatcgttt atttttttac tatatacact gccatttttt
    10201 tggctagaag aataacatcc tctgctctta aattttgtat cataaatgct tctgttttgt
    10261 tgcttcttct tcttattttg gagttttttt cgccaaactc attgagttta tttcataaaa
    10321 gtatggatgg ctatggcttt ggatttagac aaaggttatt gtcccctgaa ccatcaatgg
    10381 ctgcgtttac tttaaatatt ttcttgttga taagcatagt actggttaac tcaaagatcg
    10441 ctaaactatt tatgggaggt gcgcttgtag taggcaacct atttattggc tctaaagctt
    10501 cgctcatatt aattttgatg agtggtgtta ttgtgtttta tcttaatatg aacttaaccc
    10561 aaaaaataaa atcgttactt atattaattc ctgtatcaat tgcagttttt tatgtgtttt
    10621 taaatacgat tcttccagca ctgattgtag atgtagataa attcacaagt gttagcactc
    10681 gtttaataac agcccttgcg gctctgctga gtttaatata ctatccattg ggtgagggct
    10741 atggtaccta tagttcttat cttttgggac ctcttgattt agctgttcaa ttagcagaag
    10801 aactattgcc ttttacatta aatgtaagcg aggtcaataa aatgctaatg actggtgagt
    10861 cgttagcagc aaagtcaggt attctttttt ctgttatcca gagtggagtc gtttccctca
    10921 tattttttta cgtgatttat cgaaatgcgt ttagaaaact ctgtgctgcc aggctatcta
    10981 attataacaa gataatgatg cggttggttc tatggtattc attgttatca atcatgtttg
    11041 ctgttaatat tgaagttgtc tatgcttttt tattgccgtt tattataatt gatcattatg
    11101 ctataaatta tatgcgatct tatgattatg aataaaattt tatatatctc gctctctctt
    11161 gatgagaaaa actatggtgg cagcatcgtg tctagaaata atttaaaagc attgagggca
    11221 cttgaagata gtgaggtgaa ggaagttgca atagttaaga aattaaaagg gatatatgaa
    11281 tatgaactac aaactaatgt gtcaaaagta aaaatagcca tagataacct gaagggatat
    11341 gctggacggt taaatcgcga ttgtttattg aaaattaaaa atattataaa agtctttgag
    11401 cccaaattcg tttatcttga ctccagtttg cttgggggaa tagctgaatt atctaaagag
    11461 atatctacag acataaaagt tattacattt tttcataatg tcgagattga ttttgaatta
    11521 gaaagattga agtcaggggg aatattgtac ttgccttctc ttttgccatc ttatttagcg
    11581 gaaaaaaaag ctataaaata tagtgatata ataatttcac ttcataaaaa tgatagtgct
    11641 aggcttaagt gcatatatgg acgttcgtct gatttttgtg tcccagtttg tatagaagat
    11701 gacttgagta acgaggtaat attacataaa aataatagta cagatagtat atttagagtt
    11761 ggttttattg gaactgcatt tttcgcgaat attaaagctg cagagttcat ttcgaaaaaa
    11821 ttatcaccta ggtttttgaa taataaacaa atagaattta ttattgcggg taatggtttc
    11881 gataagtact caaaattact taatcgtaaa aatgtaaaaa ctctgggcta tattaattca
    11941 attgaagact tttataatga agttgatgtt ataatatctc ccgtcctgac gggagcaggc
    12001 atgaaagtga aaatagcgga agcgattaaa tataataaga aggtaattgc ctcgtcattt
    12061 tcattaattg gatatgagac tatgttaaat agtcaaaata ttttttcttg cattactcta
    12121 gatgattatg cgatagcaat taaaaagttg agcacacatc ctatgactca ctctgatact
    12181 cgtgaatatt atttgaaatt tttctcaagt caagcatgta ttaactattt taaatatatt
    12241 ttgaactcct aatagttaaa atgctacttc tatgtgtgtt tattgtgttg agttttttgt
    12301 tttactgctt ttatgtagta gaggatgatg ctgatgaagg tattgcaagt tagtaagttt
    12361 tacccacctg tacatggagg aattgagcaa gtcgcgtttg atatcagtga aggcatgttt
    12421 gaagaacata tacaggctgt tgatatttta tgtgtagatc cattgggacc tcgtattgat
    12481 gataggaatt ataaatatcg agtttttcga caaaagactt ttattgttct attttccact
    12541 ccgatttcaa tatcatttat aaataaatgg cgaaaaataa aagataatta tgatattatc
    12601 cacgtacatt tacctaaccc tttagctgtt ctcgctatat atctttttcc tccaaaaggt
    12661 aaaattgtat tacattggca tagtgatatt gttaagcaaa aaaaattatt cagattattt
    12721 ttacctttac aaagatggat tttggatagg tgcacttata taattgtgac tagtccagtg
    12781 tatggtcaat catctccatc gctgcaacaa tatcaaaata aattaatttg tattcctata
    12841 ggtgtagata ccagtgttat gcctgttgac ctacaagaag aaaaattaat taaaagtaag
    12901 tataaagata aaaaaataat attttcatta gggcgattgg tttattataa aggtatggat
    12961 attttaattg attcagcgaa atatttacct gatgattaca tcatattaat tggggggggg
    13021 ggcgcattat taaaaaaatt aaggaggcag atttatgata ataacttatc acacaaggtt
    13081 gttgttttgg gaagtataga ttataaatgc ttagcttcat attataaatg ctgtgatgta
    13141 ttttgtctcc catctataca tgagtctgaa gcgtttggtg tggtacaatt agaagcaatg
    13201 agtttttcaa agccattagt ttctacgaat atcccccgaa gtggtgttcc atgggttaac
    13261 gaaaatggaa catcaggaat tgttgttgag ccaaggagtg ctatagctct tgctgaaggg
    13321 attgttaaaa ctattgctaa tagtgaaaaa ttcagtaaag gagcaaaaga tagatttgat
    13381 atgctcttta ctaagagatt aatggttaat aatgtataca aattatattt aagtataaag
    13441 tgagtcaaaa tatgtcttta cttcctgtta tcattgctgg tggtaccggt agtcgtttgt
    13501 ggcctttatc acgagttaag catccaaagc aatttctcca tttaggggat gatggaacaa
    13561 tgttacagac gacattaaat cgtcttcaag ggttaaagtg tgataaccca attgtaattt
    13621 gcaatgagca gcatagattt atagttgctg agcaacttcg acaattaaat aaactcactc
    13681 agaatattat tcttgagccc gtcggccgga atactgcacc agctgtaact ctcgctgcac
    13741 tgaatgcaat acgtaataag tcaaaacaat caaaattaat tttggttctt gcagctgatc
    13801 atattataaa agacgaagat gcattttgta gaagtgtgct gagtgctatt ccatatgcaa
    13861 acaaaggaaa attaatcaca tttggtatag tgcctaatag tcccgaaaca ggctatggat
    13921 atataaaaag aggtcattta tgtagtggca ataatgctaa tttagctttt gaagtggctg
    13981 agttcgttga aaaacctaac atagatacag ctcaagagtt tctttcatct ggtaactatt
    14041 attggaatag tggtatgttt ttatttcgag ctgataggta tttagatgaa ttaaaaaaat
    14101 atagaccaga catacttgag gcctgtaaaa aatcaatgat tgaacttaat ggagatcttg
    14161 attttattcg tatcaataaa gatgcttttt gcgcttgccc agatgagtca attgattatg
    14221 ccgttatgga gaaaacgaat gatgcggttg ttatcccaat ggatgctggc tggagtgatg
    14281 tggggtcatg gtcatcttta tgggaaatga gtaataaaac cattgaagaa aatgtaatag
    14341 ttggtgatgt aataacttat gattctgaca gcagctacct ccgttcagat tcagggctgc
    14401 ttgctacagt tggggtaaaa gatcttgttg ttgttcaaac aaaagatgct gtgttagtag
    14461 ctaataggaa ttcagttcag aatgtaaaaa aaattgtcga aaggcttaaa tcagaaaatc
    14521 gtagtgaagt ttttacgcat cttgaagttt atcgtccttg gggtaaatat gagtctatcg
    14581 ataatggtga acgctatgaa gttaaacgaa tttccgtaaa acctggagag gggatttcat
    14641 tgcaaatgca tcaccatcgt tctgaacatt ggataatagt ttcgggtact gcaaaagtaa
    14701 caatttgtga tgaaacaaga attctcagtg aaaatgaatc tatttatata cctgtcgggg
    14761 cgaaacattg tttagagaat ccgggaaaaa ttatgttgga gcttatagaa gttcgctccg
    14821 gctcctatct aggagaagat gatgtcatcc gttttgccga cagatatgga agaacataaa
    14881 tgcacaataa gatcatccta gataaattaa cttgctttaa agcctacgat attcgcggaa
    14941 aactaggcga ggaactgaat gaagatatcg cttggcgcat tggtcgcgcc tatggcgaat
    15001 ttctcaaacc gaaaaccatt gtgttaggcg gcgatgtccg cctcaccagc gaaaccttaa
    15061 aactggcgct ggcgaaaggt ttacaggatg cgggcgtcga tgtgctggat atcggcatgt
    15121 ccggcaccga agagatttat ttcgccacgt tccatctcgg cgtggatggc ggcattgaag
    15181 ttaccgccag ccataatccg atggattata acggcatgaa gctggtgcgc gaaggggctc
    15241 gcccgatcag cggcgatacc ggactgcgcg acgtccagcg tctggcagaa gccaacgact
    15301 ttcctcccgt cgatgaaacc aaacgcggtc gctatcagca aatcaatctg cgtgacgctt
    15361 acgttgatca cctgttcggt tatatcaacg tcaaaaacct cacgccgctc aagctggtga
    15421 ttaactccgg gaacggcgcg gcgggtccgg tggtggacgc cattgaagcc cgctttaaag
    15481 ccctcggcgc acctgtggaa ttaatcaaag tacacaacac gccggacggc aatttcccca
    15541 acggtattcc taacccgctg ctgccggaat gccgcgacga cacccgcaat gcagtcatca
    15601 aacacggcgc ggatatgggc attgcctttg atggcgattt tgaccgctgt ttcctgtttg
    15661 atgaaaaagg gcagtttatc gagggctact acattgtcgg tcttctggca gaagcgttcc
    15721 tcgaaaaaaa tcccggcgcg aagatcatcc acgatccacg tctctcctgg aacaccgttg
    15781 atgtggtgac cgccgcgggc ggcactccgg tgatgtcgaa aaccggacac gcctttatta
    15841 aagaacgtat gcgcaaggaa gacgccatct acggtggcga aatgagcgcc caccactatt
    15901 tccgtgattt cgcttactgc gacagcggca tgatcccgtg gctgctggtc gccgaactgg
    15961 tgtgtctgaa aggaaaaacg ctgggcgaac tggtgcgcga ccggatggcg gcgtttccgg
    16021 caagcggtga gatcaacagc aaactggcgc accccgttga ggcgattaat cgcgtcgaac
    16081 agcattttag ccgtgaggcg ctggcggtgg atcgcaccga tggcatcagc atgacctttg
    16141 ccgactggcg ctttaacctg cgctcctcca acaccgaacc ggtggtgcgg ttgaatgtgg
    16201 aatcgcgcgg tgatgtaccg ctgatggaag aaaagacaaa acttatcctt gagttactga
    16261 acaagtaatt cagtaatttc atataaatgg gttttaaaaa acggaaaaga tgagatatcc
    16321 ggtgtggtat agccaaggta atgctattca gtatctctat gagtgagtta acatctatac
    16381 cacatttaag ccgcacactt ggcggtaacc acccctgaca ggagtaaaca atgtcaaagc
    16441 aacagatcgg cgtcgtcggt atggcagtga tgggacgcaa cctcgcgctc aacatcgaaa
    16501 gccgtggtta taccgtctct attttcaacc gttcccgtga taagacggaa gaaattattg
    16561 ccgaaaatcc aggcaagaaa ctggttcctt actatacggt gaaagaattc gttgaatctc
    16621 ttgaaacgcc tcgtcgcatc ctgttaatgg tgaaagcagg tgcaggcacg gatgctgcta
    16681 ttgattccct taagccatac ctcgataaag gtgacatcat cattgatggt ggtaatacct
    16741 tcttccagga caccattcgt cgtaaccgtg agctttctgc cgaaggtttt aacttcatcg
    16801 gtaccggtgt ttccggcggt gaagaggggg cgctgaaagg gccttccatc atgcctggtg
    16861 gccagaaaga agcctatgaa ctggttgcgc cgatcctgac caaaatcgcc gccgttgctg
    16921 aagatggcga accgtgcgtt acctatatcg gtgccgatgg cgcgggtcac tatgtgaaga
    16981 tggttcacaa cggtattgaa tacggtgata tgcaactgat tgctgaagcc tattctctcc
    17041 tgaaaggcgg cctgaatctc tctaacgaag aactggcaca gacctttacc gagtggaata
    17101 acggtgaact gagcagctac ctgatcgaca tcaccaaaga tatcttcacc aaaaaagatg
    17161 aagacggtaa ctatctggtt gatgtgatcc tggatgaagc ggctaacaaa ggtaccggta
    17221 aatggaccag ccagagcgcg ctggatctcg gcgaaccgct gtcgttgatt actgagtcag
    17281 tgtttgcacg ttatatctcg tctctgaaag atcagcgcgt ggccgcgtct aaagttctct
    17341 ctggtccgca agcacagcca gctggcgata aagctgagtt catcgagaaa gttcgtcgtg
    17401 cgctgtatct gggcaaaatc gtttcttacg ctcaggggtt ctctcaactg cgtgcggcgt
    17461 ctgaagagta caactgggat ctgaactacg gcgaaatcgc gaagattttc cgtgctggct
    17521 gcatcatccg tgcgcagttc ctgcagaaaa tcaccgatgc atatgccgaa aatccgcaga
    17581 tcgctaacct gctgctggct ccgtacttca agcaaattgc cgatgactac cagcaggcgc
    17641 tgcgtgatgt cgttgcttat gcagtacaga acggtatccc ggttccgacc ttcgctgctg
    17701 cggttgccta ttatgacagc taccgtgccg ctgttctgcc tgcgaacctg attcaggccc
    17761 agcgtgacta
//