LOCUS AB811624 16181 bp DNA linear BCT 13-MAY-2015
DEFINITION Escherichia coli DNA, O-antigen biosynthetic locus, strain: U18-41.
ACCESSION AB811624
VERSION AB811624.1
KEYWORDS .
SOURCE Escherichia coli
ORGANISM Escherichia coli
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
Enterobacteriaceae; Escherichia.
REFERENCE 1
AUTHORS Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
Hayashi,T. and Thomson,N.R.
TITLE A complete view of the genetic diversity of the Escherichia coli
O-antigen biosynthesis gene cluster
JOURNAL DNA Res. 22 (1), 101-107 (2015)
PUBMED 25428893
REMARK DOI:10.1093/dnares/dsu043
REFERENCE 2 (bases 1 to 16181)
AUTHORS Iguchi,A., Iyoda,S. and Ohnishi,M.
TITLE Direct Submission
JOURNAL Submitted (29-MAR-2013) Contact:Atsushi Iguchi University of
Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
Miyazaki, Miyazaki 889-2192, Japan
FEATURES Location/Qualifiers
source 1..16181
/organism="Escherichia coli"
/mol_type="genomic DNA"
/strain="U18-41"
/db_xref="taxon:562"
/note="serogroup:O50"
gene <1..793
/gene="galF"
CDS <1..793
/gene="galF"
/note="O050_01"
/codon_start=2
/transl_table=11
/protein_id="BAQ01002.1"
/translation="PMIQYIVDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLE
LRVKRQLLAEVQSICPPGVTIMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVID
DASADPLRYNLAAMIARFNETGRSQVLAKRMPGDLSEYSVIQTKDPLDREGKVSRIVE
FIEKPDQPQTLDSDIMAVGRYVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQAVD
AMLMTGDSYDCGKKMGYMQAFVKYGLRNLKEGAKFRKGIEKLLSE"
gene 1166..2251
/gene="rmlB"
CDS 1166..2251
/gene="rmlB"
/note="O050_02"
/codon_start=1
/transl_table=11
/product="dTDP-glucose 4,6 dehydratase"
/protein_id="BAQ01003.1"
/translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
EVSDSERYSFEHADICDAEAMARIFSQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
GTYVLLEAARNYWSGLDDEKKKNFRFHHISTDEVYGDLPHPDEVNNNEALPLFTETTA
YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
LANTNWVENVKSGAYQSWIEQNYEGRQ"
gene 2251..3150
/gene="rmlD"
CDS 2251..3150
/gene="rmlD"
/note="O050_03"
/codon_start=1
/transl_table=11
/product="dTDP-4-dehydrorhamnose reductase"
/protein_id="BAQ01004.1"
/translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTNYCGDFSNPE
GVAETVKKIRPDVIVNAAAHTAVDKAESEPDFAQLLNATSVEAIAKAANEVGAWVIHY
STDYVFPGSGDAPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALKKPEVAGLYHLVAS
GTTTWHDYAALVFEEARKAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
LVLPDWQVGVKRMLNELFTTTAI"
gene 3209..4084
/gene="rmlA"
CDS 3209..4084
/gene="rmlA"
/note="O050_04"
/codon_start=1
/transl_table=11
/product="glucose-1-phosphate thymidylyltransferase"
/protein_id="BAQ01005.1"
/translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEDFIGG
DDCALVLGDNIFYGHDLPKLMEAAVNKESGATVFAYHVNDPERYGVVEFDNNGTAISL
EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
IKNQYGQYLLKMISE"
CDS 4099..4512
/note="O050_05"
/codon_start=1
/transl_table=11
/product="putative dTDP-6-deoxy-3,4-keto-hexulose
isomerase"
/protein_id="BAQ01006.1"
/translation="MMDIKLISLQKHGDERGALIALEEQRNIPFEVKRIYYILETLNG
VRRGFHAHKVTRQLAIVVKGACKFHLDNGKETKQVELNDPTIALLIEPYIWHEMYDFS
DDCVLLVIADDFYKESDYIRNYDDFIRRVNSIENS"
CDS 4499..4966
/note="O050_06"
/codon_start=1
/transl_table=11
/product="putative acetyltransferase"
/protein_id="BAQ01007.1"
/translation="MRIHKLSDVQTTSIGDGTTIWQFVVILKGAVIGNNCNICANTLI
ENNVVIGNNVTVKSGVYIWDGVKIEDNVFIGPCVAFTNDKYPRSKVYPDEFLQTIIRK
GASIGANATILPGIEIGEKAIVGAGSVVTKNVPPCAIVVGNPARFIKWVEDNE"
CDS 4959..6065
/note="O050_07"
/codon_start=1
/transl_table=11
/product="putative aminotransferase"
/protein_id="BAQ01008.1"
/translation="MNKIDFLDLFAINQRQHKELVSAFSRVLDSGWYIMGEELEQFEK
EFAEYCGVKYCIGVANGLDALILVLRAWKELGYLEDGDEVLVPANTYIASILAITENK
LVPVLVEPDIETYNINPALIENYITEKTKAILPVRLYGLLCNMPEISAIARKYNLLIL
EDCAQAHGAIRDGRKAGAWGDAAGFSFYPGKNLGALGDAGAVTTNNAELSSTIKALRN
YGSHKKYENIYQGLNSRLDELQAALLRVKIHTLPEDTAIRQRIAEKYIREIKNPAITL
PVYEGQGAHVWHLFVVRIANREKFQSYLLEKGIKTLIHYPLPPHKQQAYQNMSSLSLP
ITEQIHDEVISLPISPVMSEDDVNYVIKMVNDYK"
gene 6065..7327
/gene="wzx"
CDS 6065..7327
/gene="wzx"
/note="O050_08"
/codon_start=1
/transl_table=11
/product="O-antigen flippase"
/protein_id="BAQ01009.1"
/translation="MKKFLQVTILSAIYTFIKMIAGFIIGKVVAIYTGPSGVAMLGQV
QSLITIVAGTTSAPVSTGLVRYTAENWQEGQEACAPWWRACLRVTLFLFLLIIPVVII
LSKNISELLFSDGQYTWLIIFACCILPFSIINTLIASVLNGQQFYKQYILVGMFSVFI
STMFMILLIVAYNLKGALIATAINSAIAGLVLVLFCLNKSWFRFKYWWGKTDKDKIIK
IIHYTLMALVSVISMPTALMCIRKILIAKTGWEDAGQWQAVWKISEVYLGVVTIALST
YFLPRLTIIKTSFLIKKEVNSTILYIISITSFMALSIYLFRDLVITVLFTEQFRSARE
LFLLQLIGDVIKIAGFLYAYPLQSQGHTKLFISSEVIFSMLFIITTYIFVVNYGVHGA
NISYVITYSLYFVFAFVFTNFINVRRNN"
CDS 7344..8393
/note="O050_09"
/codon_start=1
/transl_table=11
/product="putative glycosyltransferase"
/protein_id="BAQ01010.1"
/translation="MKIIIPVLGFGRAGGERVLSKLATELMNYGHDVSFVVPDNRTNP
YYATTAKIVTSKSSQNRVKILRIIKNYYNLWRKCIELNPDAVVASFHLTAYLVALLPI
TRRKKYYYIQAYEVNFFDNIIWKLIAGLTYYLPLKKILNSPNLLPHKHDDFIGVVPAG
VDLNVFYPKPSNRLLNGHTSIGIIGRKEKHKGTSEIISVLCSLENKAGIIINIAIYLE
EVDKQRLIAAGFQVNFFPITSDLELASFYRSNDIMIAVGLIEDGAFHYPCAESMACGC
LVISNYAPLTETNSVLKLVKFDACKLGEAINLCLNLDLEEKSKEIQSNISVLNKYDWK
IVGETFNSLLLDANK"
gene 8414..8971
/gene="rmlC"
CDS 8414..8971
/gene="rmlC"
/note="O050_10"
/codon_start=1
/transl_table=11
/product="dTDP-4-dehydrorhamnose 3,5-epimerase"
/protein_id="BAQ01011.1"
/translation="MNIVKTDIPDLIVLEPKVFSDERGFFMESYNQIEFEKAIGRHVN
FVQDNHSKSSKGVLRGLHYQLAPYAQAKLVRCVVGQVFDVAVDLRKNSPTFKKWFGIT
LSAENKRQLWIPEGFAHGFLVTSDEAEFIYKTTNYYAPGHQQAIIYNDPILNIDWPFC
SSALSLSQKDQEAKLFSELLDSELF"
gene 9040..10002
/gene="wzy"
CDS 9040..10002
/gene="wzy"
/note="O050_11"
/codon_start=1
/transl_table=11
/product="O-antigen polymerase"
/protein_id="BAQ01012.1"
/translation="MTENSPKYKHDKSLISFIYLFFIFTLIVGFIIAITQFLGRSRDY
DNYIQIFSGKEGEGVLELFYRGLMLITTSYETIIFIILTCSFFIKARFLANYSRNFSG
LTLFFIYYASVALWVLDYTQFRNGLCISILMFSVYYLFINKPTYFYFSVLCAIATHWS
ALPFLLLYPFVYSTKIRRLGYFCFSILVLIAISGEGKEIISFIRNFGVGQKIGNEAGV
NLINSLSLTAISWFIISYISSIGNERRNLRLFFCYGVMQYVTFSLFSLPVMAFRILEM
YFFLMLTIGVFIKQKKNYYFVFCKVLILLYLTYYYHMVFGVINV"
CDS 9995..10882
/note="O050_12"
/codon_start=1
/transl_table=11
/product="putative glycosyltransferase"
/protein_id="BAQ01013.1"
/translation="MCKAKVLAIIVTYNPEIIRLTECINSLAPQVERIILVDNGSNNS
DLIKNISINNLEIILLSENKGIAFAQNHGVKKGLEAKEFDYLFFSDQDTCFPSDVIEK
LKSTFTKNNKKGKNVACASPFFKDHRSNYMHPSVSLNIFTSTKVICSEVDDDLYPSHV
IASGMLMSREAWRVVGPFCEKLFIDWVDTEWCWRALANNMIIVQTPSVIISHELGYGQ
KIFAGRSVTIHNSFRNFYKIRNAIYLMLHSNYSFKYRYHAFFHATKNVVFEILYSKEK
LNSLKVCFKAVRDGMFNNF"
CDS 10913..11989
/note="O050_13"
/codon_start=1
/transl_table=11
/product="putative glycosyltransferase"
/protein_id="BAQ01014.1"
/translation="MEENNMKTVAVVGTVGVPACYGGFESLVQNLIDYQSDGIQYQIF
CSSKKYDKKFKNYKNAELIYLPINANGVSSIIYDIMCLIICLFKRPDVVLILGVSGCL
FLPIYKLFSKSKIIVNIDGLEWRRNKWGTFAKKFLKISEAISIRIADIIISDNQAIAD
YVENKYKKKSVVIAYGGDHATNLSTPIDNDQKKEGYYLGLCRIEPENNIEMILNAFIN
TDKKIKFMGNWDNSEYGRQLKKYYSNYPNITLLEPNYNIEELYKLRKNCLAYIHGHSA
GGTNPSLVEAMHFNIPIFAFDCDFNRYTTNNLAHYFNDSEQLSLLAESLSFGNLKCRV
LDLKNYAEDMYNWRHIAAMYESIY"
CDS 12168..12722
/note="O050_14;
transposase of ISEc1"
/pseudogene="unknown"
/codon_start=1
/transl_table=11
CDS 13002..13304
/note="O050_15;
transposase of ISEc1"
/pseudogene="unknown"
/codon_start=1
/transl_table=11
CDS 13565..14059
/note="O050_16;
transposase of ISAs1 family"
/pseudogene="unknown"
/codon_start=1
/transl_table=11
CDS 14246..14599
/note="O050_17;
transposase of ISAs1 family"
/pseudogene="unknown"
/codon_start=1
/transl_table=11
ORIGIN
1 gccaatgatt cagtacattg ttgacgagat tgtggctgca gggatcaaag aaatcctcct
61 ggtaactcat gcgtccaaga acgcagtcga aaatcacttc gatacatctt atgaattaga
121 atctctcctt gaactgcgcg tgaagcgcca gcttctggcg gaagtacaat ctatctgccc
181 gccaggtgtg accattatga acgtgcgtca gggggaacct ttaggtctgg gccactcgat
241 tttgtgtgcc agaccagcca ttggcgataa cccgtttgtc gtggtgctgc cggatgttgt
301 tatcgacgac gccagtgccg atccgctgcg ttacaatctg gccgcaatga ttgcgcgctt
361 caatgaaact ggccgcagcc aggttctggc aaaacgcatg ccgggcgatc tctctgaata
421 ctccgtcatc cagaccaaag atccgcttga tcgcgaaggc aaagtcagcc gcattgttga
481 atttatcgaa aaaccggatc agccgcagac cctggactca gacatcatgg ctgtagggcg
541 ttatgtgctt tctgccgata tttggcctga actggagcgt actcaacctg gagcatgggg
601 acgtattcag ttgactgatg ccattgctga gttggcaaaa aaacaagcag ttgacgcaat
661 gctgatgact ggggacagtt acgactgtgg taagaaaatg ggttatatgc aagcgtttgt
721 gaagtatgga ctacgcaacc tgaaagaagg cgctaagttc cgcaaaggca ttgagaagtt
781 gttaagcgag taacaaattc ctgacaggaa gtgtcagtca gtaagtaatt aaaaacggca
841 gtgaagattc gtagcataag ttttgctcat tgaatctcac tgccgttgtt gtttttataa
901 atgcaatgaa aacaggatgt tagcttgtaa tcatagttca aatttttcca ggattttcct
961 tgtttctaca gctgtttggt aagacaatta gcgtttgaat ttttcggatt tggcacgagc
1021 gggatacgct cgtcacatcg tagaaatgta cgcagtgctc tggtagctgt taagccaggg
1081 gcggtagcgt gcattaatac ttctattaat caaatctcga gcagtctatt tcacagtatg
1141 ctctctggct atatggaata aaaaagtgaa gatacttgtt actggtggcg caggctttat
1201 tggttctgct gttgttcgtc acataataaa taatacgcaa gatagtgttg ttaatgtcga
1261 taaattaaca tacgccggaa acctggaatc gctcgctgaa gtttctgatt ctgagcgtta
1321 ttcatttgag catgcagata tctgcgatgc cgaagcgatg gcgcgtattt tttcacaaca
1381 ccagccagat gcggtgatgc accttgcggc tgagagccac gttgaccgct caataactgg
1441 cccggcggca ttcattgaaa ctaatattgt aggtacttac gtacttttag aagctgcgcg
1501 caattattgg tctggtctgg atgatgaaaa gaaaaaaaac ttccgctttc atcatatttc
1561 tactgatgaa gtatatggtg acttacccca tccggatgaa gtaaataata acgaagcgtt
1621 accgctattt acggaaacga cagcctacgc gccaagtagc ccgtattctg cttctaaagc
1681 ttccagcgat catttggttc gcgcatggaa acgtacgtat ggtttaccga ccattgtgac
1741 taattgctcg aacaactatg gtccgtatca cttcccggaa aagcttattc cattggttat
1801 tcttaatgca ctggaaggta aggcattacc tatttatggc aaaggggatc aaattcgcga
1861 ctggttgtat gtagaggatc atgctcgtgc gttatatacc gtcgtaaccg aaggtaaagc
1921 gggtgaaact tataacattg gcggacacaa cgaaaagaaa aacatcgatg ttgtgctgac
1981 tatttgtgat ttgttggatg agattgtacc gaaagagaaa tcttatcgtg agcaaattac
2041 ttatgttgct gatcgcccag ggcatgatcg ccgttatgca attgatgccg ataaaattag
2101 ccgcgaattg ggctggaaac cacaggaaac gtttgagagc gggattcgca aaacggtgga
2161 atggtatctg gctaatacaa attgggttga gaatgtgaaa agcggtgctt atcagtcatg
2221 gatcgaacaa aactatgagg gccgtcagta atgaatatcc tgcttttcgg caaaacaggg
2281 caggtgggtt gggaactgca gcgtgctctg gcgccgctgg gtaatctgat cgctcttgat
2341 gttcactcca ctaattattg tggagatttc agcaaccccg aaggtgtggc agaaaccgtc
2401 aaaaaaattc gtcctgacgt tattgttaat gctgctgctc acactgcagt agataaagca
2461 gaatcagaac cggatttcgc acaattactt aacgcgacaa gcgtcgaagc gattgcaaaa
2521 gctgctaatg aagtcggggc ctgggttata cactactcta ctgattatgt tttcccaggc
2581 agtggtgacg cgccatggct ggaaacggat gcaacagcac cgctaaatgt ttacggtgaa
2641 acaaaattag ctggggaaaa ggcattacaa gaacattgcg caaagcatct tattttccgt
2701 accagctggg tatacgctgg taaaggaaat aactttgcta aaacgatgtt gcgtttggca
2761 aaagaacgcg aagaactggc tgtgataaac gatcagtttg gcgcaccaac aggtgctgaa
2821 ttgctggctg attgcaccgc tcatgccatt cgcgtggcat taaaaaaacc agaagtcgct
2881 ggcttgtacc atctggtagc aagtggcaca acaacctggc acgattatgc tgcgctggtt
2941 tttgaagagg cgcgcaaagc agggattaat cttgcactta acaaacttaa cgccgtgcca
3001 acaacggcct atcccacacc agcccgtcga ccccataact ctcgcctcaa tacagaaaag
3061 tttcagcaga actttgcgct tgtcttgcct gactggcagg tgggcgtgaa acgtatgctc
3121 aacgaattat ttacgactac ggcaatttaa caaatttttg catctcgctc atgatgccag
3181 agcgggatga attaaaagga atggtgaaat gaaaacgcgt aaaggtatta ttctggctgg
3241 tggttccggc actcgtcttt atcctgtgac gatggcagtg agtaaacaat tgctgccgat
3301 ttatgataag ccgatgattt attatccgct ttcaacgctt atgttagcgg gtattcgcga
3361 tattcttatt attagtacgc cacaggatac accgcgtttc caacaattat tgggggacgg
3421 gagccagtgg ggtcttaatc tacagtataa agtacaaccg agtccggatg gcctggcgca
3481 agcgtttatt attggcgaag actttattgg tggtgatgat tgtgcactcg tacttggcga
3541 taatatcttc tatggacacg acttgccgaa attgatggaa gctgctgtta acaaagaaag
3601 cggtgcaacg gtatttgctt atcacgttaa tgatcctgaa cgctatggtg tcgtggagtt
3661 tgataataac ggtacggcaa ttagcctgga agaaaaaccg ctggagccaa aaagcaacta
3721 tgcggttact gggctttatt tctatgacaa tgacgttgtg gaaatggcta aaaaccttaa
3781 gccttctgcc cgtggcgaac tggaaattac cgatattaac cgtatttata tggaacaagg
3841 acgtttgtct gtagccatga tggggcgtgg ctatgcatgg ttggatacag ggacgcatca
3901 aagccttatt gaagcaagta acttcattgc aacaattgaa gagcgtcagg gattaaaggt
3961 atcttgcccg gaagagattg cttaccgtaa agggtttatt gatgccgagc aggtgaaagt
4021 attagccgaa ccgcttatca agaatcaata tggtcaatat ttgctgaaaa tgatcagcga
4081 atagtatatg ggaactcaat gatggatatt aaattaatct ctttgcaaaa acatggggat
4141 gagcgcggtg cattaattgc tcttgaagag caacgaaata tacctttcga agtcaaaaga
4201 atatattaca tacttgagac tcttaatgga gtaagacgcg gatttcatgc gcacaaggtt
4261 actcgtcagt tagctattgt agtcaaggga gcttgtaaat ttcatctgga taatggtaaa
4321 gaaacaaagc aggtggaact taatgatcca acaattgcgt tgctgataga accctatata
4381 tggcatgaaa tgtatgattt tagtgatgat tgtgtgctgc ttgtaattgc ggatgatttc
4441 tataaagagt ctgattatat ccgcaattat gatgatttta ttagaagagt aaattcaatt
4501 gagaattcat aagctaagtg acgtccagac aacatcaatt ggtgatggaa caactatctg
4561 gcagtttgtt gtgatactaa aaggtgctgt aattggtaat aattgcaaca tctgtgcaaa
4621 taccttaatt gaaaataacg ttgtaattgg taacaatgtc acagtcaaaa gcggtgtgta
4681 tatttgggat ggcgttaaaa tagaggataa tgtttttatt ggtccttgtg tagcatttac
4741 aaatgataag tatcctcgct ctaaagtcta tcctgatgaa tttttgcaaa caataatacg
4801 caaaggagca tcaataggtg ctaacgcaac catcctgcca ggaattgaaa ttggtgaaaa
4861 agcaatcgtt ggtgcgggga gtgttgtaac caaaaatgta ccgccatgcg caatagtagt
4921 aggtaatcca gctcgattta ttaaatgggt agaggataat gaataaaatt gattttttag
4981 atctttttgc aattaaccag cgacagcaca aagaattagt ctctgcgttt agtagggtgc
5041 tagattctgg ttggtatatc atgggcgaag aacttgagca gttcgagaaa gagttcgcag
5101 aatactgtgg agttaagtat tgcattggtg tagcaaatgg ccttgatgcg ttgatactag
5161 tattgagggc atggaaagaa cttggctatc ttgaagacgg tgacgaggta ttagtaccgg
5221 caaatacata tattgcttct attcttgcta taacagagaa caaacttgtt cctgttcttg
5281 ttgaaccaga tatagaaact tataatatta atcctgcttt aattgaaaat tacattacgg
5341 aaaaaactaa agcaatatta ccggttcgct tatatggtct attgtgcaat atgccagaaa
5401 ttagtgcaat cgccagaaaa tataatctgt tgattcttga agattgtgca caagcacatg
5461 gtgcaatacg tgatggtcgc aaagctggag cttgggggga tgctgcagga tttagttttt
5521 atccaggaaa aaaccttgga gctttggggg atgcgggagc tgttactaca aataatgcag
5581 aattatcctc aactataaaa gctttgcgaa attatgggtc acataagaaa tatgaaaata
5641 tttatcaggg attgaatagt cgattggatg aactgcaagc agccttattg cgtgtaaaaa
5701 tccatacatt accggaagat actgcgattc ggcaaaggat tgctgaaaaa tatattcgtg
5761 aaataaaaaa ccctgcgatt acgttaccag tgtacgaagg ccaaggtgcg cacgtttggc
5821 atttatttgt agtaagaatc gctaatcgtg aaaaattcca gtcatactta ttagagaagg
5881 gtatcaaaac cttaattcac tatccattac caccccataa gcagcaagca tatcaaaata
5941 tgtctagcct tagccttcca attactgagc aaattcatga tgaagtcatt tctttaccta
6001 taagtccggt aatgagtgaa gatgatgtca attatgtaat caaaatggtc aatgattaca
6061 agtaatgaaa aaatttcttc aggtaactat attatccgct atctatacat tcattaaaat
6121 gattgcgggt tttatcatcg gtaaggtagt agcaatttat acagggccat caggggtagc
6181 aatgcttggc caagtgcaaa gtttaatcac aatagttgca ggtactacct ctgcacctgt
6241 aagcactggc cttgttcgat atactgcgga aaattggcaa gaaggacaag aagcatgcgc
6301 gccatggtgg cgcgcatgct taagggttac tctgttttta ttcttgctta ttattcccgt
6361 tgttattata ttgtcgaaaa atattagtga gttacttttt agcgatggac aatacacatg
6421 gttaatcatt ttcgcatgtt gtatattgcc attctccatt ataaatacat tgatcgcttc
6481 agttttaaat ggtcaacaat tttataagca atatatattg gttgggatgt tttctgtatt
6541 catttctact atgtttatga ttttgttgat tgtagcttat aatcttaaag gtgcattgat
6601 tgccacagct ataaatagtg ctattgctgg tcttgtattg gttttatttt gtctcaataa
6661 atcttggttt agatttaaat attggtgggg taaaacggat aaagacaaaa ttataaaaat
6721 tattcattat actctgatgg ctctggtttc tgttatctcc atgcctacag cattgatgtg
6781 tattagaaaa atattgattg ctaaaactgg ttgggaggat gcagggcaat ggcaggccgt
6841 atggaagata tctgaggttt atcttggtgt tgtgacaatt gctttgtcaa catatttctt
6901 accaagattg acaattataa aaacaagttt ccttataaaa aaagaagtaa atagtactat
6961 attatacata atatctatta cttcattcat ggcgttgagt atctatttat tccgcgattt
7021 ggtaataaca gttttattta ctgaacagtt tcgctcagct cgtgaattat ttttattaca
7081 acttataggg gatgtaataa aaattgctgg gtttctttat gcataccctc ttcaaagtca
7141 ggggcatact aaactattca tcagttcaga agtgattttt tctatgctct ttatcattac
7201 cacctatatt tttgttgtaa attatggagt acatggtgct aacataagtt atgtcattac
7261 atatagttta tattttgtgt ttgcatttgt gtttactaat tttattaatg ttagaagaaa
7321 taattaaaaa cagaggttga attttgaaaa taattatacc tgtcttagga tttggcaggg
7381 ctggtggtga aagagttctt tctaagctgg caactgaatt gatgaattat ggacatgatg
7441 taagttttgt tgttccagat aatagaacta atccatatta tgctaccaca gcaaaaattg
7501 tcacgagtaa atctagtcaa aaccgtgtaa aaatattgag aatcattaaa aattactata
7561 atctgtggcg taaatgcata gagttaaatc ctgatgctgt agttgctagt tttcatttga
7621 ctgcctatct tgtcgcatta ttaccaatca cccgtcgtaa gaaatattat tatattcagg
7681 cgtatgaagt taattttttt gataatataa tatggaaatt aatagcgggt ttaacatatt
7741 atttaccgct taaaaaaata ctaaatagtc ctaatttgct tcctcataaa catgatgatt
7801 ttataggagt agttcctgca ggagtagatt taaacgtttt ctatccgaaa ccatcaaata
7861 ggttattaaa tggtcacaca tcaataggga ttattggtag aaaagagaag cacaaaggaa
7921 ctagcgaaat tatttcagta ttgtgttcac tggaaaataa agctggaatt ataatcaata
7981 ttgcgatcta tcttgaagaa gttgataagc agcgtttaat cgctgccggg tttcaggtta
8041 atttttttcc gattacttct gatttagaat tggcatcctt ttatcgaagc aatgacatca
8101 tgattgctgt tgggttaatt gaagatggcg ctttccatta tccttgtgct gaatcaatgg
8161 cttgtggttg tcttgttatt tcaaattatg cgccacttac tgaaactaac agtgtactta
8221 aattagtcaa gtttgatgct tgcaaacttg gtgaagcaat taatctttgt ctcaatcttg
8281 acctagaaga aaaaagcaaa gaaatccaat ctaatatttc tgtgttgaat aaatatgact
8341 ggaaaattgt tggtgaaact ttcaatagtt tattgttaga tgcaaataaa tagtatacgt
8401 tgatggggaa aatatgaata ttgttaaaac tgatattcca gatctgatcg ttcttgaacc
8461 aaaagtgttt agtgatgaac gcggcttttt tatggagagt tataatcaga ttgaatttga
8521 gaaggcaata ggaaggcacg taaattttgt tcaggataat cattcaaaat ctagtaaagg
8581 cgtactacgt gggttgcatt atcaattagc accgtatgca caggctaaat tagttcgatg
8641 tgttgtaggt caggtatttg atgttgctgt tgatcttaga aaaaattcac caacgttcaa
8701 aaaatggttt ggaataaccc tttccgcaga aaataaacga caattatgga tacccgaagg
8761 atttgctcat ggtttcttgg tgaccagtga tgaagctgag ttcatttata agacaactaa
8821 ctactatgct cctggtcatc agcaagcaat tatttacaat gatcctattt taaacatcga
8881 ttggcctttc tgcagtagtg ctctgtcatt atcacaaaaa gatcaagaag caaaattatt
8941 ttcagaatta ttggacagtg aactgttcta ataaagtgtg ccaccttatc cgtctgaagg
9001 ataggtggtt gcttatattt ttttgagtat gtttgtataa tgacagaaaa tagtccgaaa
9061 tataaacacg ataaaagctt aataagtttt atctacttat tttttatatt tacacttatt
9121 gtaggcttta ttatcgcaat tacccagttt ttggggcgaa gtagagacta tgataattat
9181 atacagatct tttctggtaa agaaggggag ggggttcttg aattatttta tcgcggattg
9241 atgttaataa cgaccagcta tgaaactatc atttttataa ttttaacatg ttcttttttt
9301 ataaaggcaa ggtttctcgc taactattcg cgtaattttt caggcttgac cttattcttt
9361 atttattatg caagcgttgc actttgggtt ttagattata ctcaattcag aaatggtcta
9421 tgtatttcca ttttaatgtt ttccgtatac tatttattta taaataaacc gacttatttt
9481 tatttctcgg tattatgtgc aattgcaact cattggtctg ctttgccttt tttgctttta
9541 tatccttttg tctattcaac aaaaataaga cgccttggtt atttttgttt cagtattctt
9601 gttttgattg cgatctcagg agaaggaaaa gagatcatat cttttataag aaattttgga
9661 gtgggacaaa aaataggaaa tgaagctggt gtaaatttaa taaattcatt atcccttacc
9721 gctatttcct ggtttattat tagttacata tcaagcattg gaaatgaaag gagaaattta
9781 aggcttttct tttgttatgg tgtcatgcaa tacgtgactt ttagcctttt ctctctacct
9841 gttatggctt tccgtatttt ggaaatgtat tttttcctta tgctaaccat tggggtgttt
9901 attaagcaaa aaaagaatta ttattttgtt ttttgcaaag tgttaatttt attgtatcta
9961 acatactatt atcatatggt ctttggagtg attaatgtgt aaggctaagg tgttggctat
10021 aattgttact tacaacccgg aaattattcg attgacggaa tgtattaact ctttagcccc
10081 acaagttgag agaataattc ttgtagataa tggctcaaat aatagtgatt tgataaaaaa
10141 tatcagtatt aataaccttg aaattatttt actttcggaa aacaaaggca ttgcatttgc
10201 tcagaaccat ggtgttaaga agggcctgga agcaaaagag tttgactatt tatttttctc
10261 agatcaggat acttgctttc ctagcgatgt tattgaaaaa cttaagagta catttacgaa
10321 aaataataaa aaaggtaaaa atgttgcttg tgcttctcct ttttttaaag accatcgttc
10381 aaattatatg catccgtcag tcagcctaaa tatttttacg agtacaaaag ttatatgtag
10441 tgaagtagac gatgatcttt atccctcgca tgttattgct tctgggatgt taatgtctcg
10501 tgaagcatgg cgcgtcgtcg gaccattttg tgaaaaactc tttatagact gggttgatac
10561 agaatggtgt tggcgtgcat tagctaataa tatgattatt gttcagacac catcagtcat
10621 catttctcat gaacttgggt atgggcagaa aatttttgct ggtcgatctg ttacaataca
10681 taattctttc agaaattttt ataaaatacg caatgcaata tacttaatgc tgcattcaaa
10741 ttatagcttc aagtatcgtt atcatgcttt ttttcatgcg acaaagaatg ttgtatttga
10801 aattttatat tcgaaagaaa aattaaattc actgaaggtt tgttttaaag ctgtacgtga
10861 tggtatgttc aataattttt aatacgaaaa tagttaggct caaggtgttt aaatggaaga
10921 aaataatatg aagacggtcg ctgtagttgg cacagtgggt gttcctgctt gttatggtgg
10981 gttcgaatca cttgttcaga atctaattga ttatcaatct gatggtatac aatatcagat
11041 attttgctct tcaaaaaaat atgataaaaa atttaaaaat tataaaaatg cagaattaat
11101 ctatttgccg ataaatgcca atggcgtctc tagcataatt tatgatatta tgtgtttaat
11161 tatttgttta ttcaaaaggc cagatgttgt tttaatattg ggggtgtctg gttgtttatt
11221 tctaccaatt tataaactat tttcaaaatc aaagattatt gtcaatattg atgggcttga
11281 atggcgtaga aataaatggg gaacgtttgc taagaaattt cttaaaatat ctgaggcgat
11341 atctattaga atagctgata ttatcatttc agataatcaa gcaatagctg attatgtgga
11401 aaataagtac aagaaaaaaa gtgtagttat agcttatggc ggagatcatg ccactaatct
11461 tagtacaccg atagacaatg atcaaaaaaa agaaggttat tatttggggc tttgtaggat
11521 agagcctgag aataatatag aaatgattct gaatgccttc attaatacag ataaaaaaat
11581 taaatttatg ggtaattggg ataacagcga gtatggacgc cagctaaaaa aatattattc
11641 aaactatcca aatatcaccc tactagaacc taactataat attgaagagc tttataaact
11701 aagaaaaaat tgtcttgcat acattcatgg acactcggct ggtggaacaa acccttcttt
11761 agttgaagcg atgcatttta atattcctat ttttgctttc gattgtgact ttaatcgtta
11821 cacaactaac aatttagctc attactttaa tgattctgaa caacttagct tattagcaga
11881 aagtttgtct tttggaaatc ttaaatgtcg agtattagat ttaaaaaatt atgctgaaga
11941 tatgtataac tggaggcata tagctgctat gtatgaatct atttattaaa cgcattaaca
12001 ataatataat tgaccttata tagcagggaa agatcacgta acgctgcttt tttgtactaa
12061 ataattcgca ttttatgttt aaaaattgag atatccctta ttacctaaag ctgtttttta
12121 ttgcttatac atgatcaaat actccttaca taattaagga gaacaaaatg gaacttaaaa
12181 aattgatgga acatatttct attatccccg attacagaca agcctggaaa gtggaacata
12241 aattatcgga tattctactg ttgactattt gtgccgttat ttcgggtgca gaaggctggg
12301 aagatataga ggattttggg gaaacacatc tcgatttttt gaagcaatat ggtgattttg
12361 ataatggtat tcctgttcac gatactattg ccagagttgt atcctgtatc agtcctgcaa
12421 agtttcacga gtgctttatt aactggatgc gtgactgcca ttcttcagat gataaagacg
12481 tcatcgcaat tgatggaaaa acactccggc attcttatga caagagtcgc cgcaagggag
12541 cgattcatgt cattagtgcg ttctcaacaa tgcacagtct ggtcatcgga cagatcagga
12601 cggatgagaa atccaatgaa attacagcta tccctgaact tcttaacatg ctggatatta
12661 aaggaaaaat catcacaact gatgcgatgg ggtgccagaa agatattgca gagaagatat
12721 aaaaacaggg cggtgattat ttattcgctg taaaaggaaa tcaggggcgg cttaataaag
12781 cctttgagga aaaatttcca ctgaaagaat taaataatcc agaacatgac agttacgcca
12841 ttagtgaaaa gagtcacggc agagaagaaa tccgtcttca tattgtttgc gatgtccctg
12901 atgaacttat tgatttcaca tttgaatgga aaggactgaa gaaattatac gttgcagtct
12961 cctttcggtc cataatagca gaacaaaaga aagagccaga aatgacggtc agatattata
13021 tcagttctgc tgatttaacc gcagagaagt tcgccacagc aatccgaaac cactggcacg
13081 tggagaataa gctgcactgg cgtctggacg tggtaatgaa tgaagacgac tgcaaaataa
13141 gaagaggaaa cgccgcagaa ttattttcag ggatacggca catcgctatt aatattttaa
13201 cgaatgataa ggtattcaag gcagggttaa gacgtaagat gcgaaaagca gccatggaca
13261 gaaactatct cgcgtcagtc cttgcgggga gcgggctttc gtaatcttgc cctgccttat
13321 atagctatca gagtaaaaaa tgtaacgctt attattttgt taaataatac gcattatata
13381 ttcaataatt aaaatgctct taattaaata aaactattta attaataata taaaatttca
13441 ttatgttact taagagatga aaattataac ttaaaaaagt gatggaacat atttctatta
13501 tccctgatta cagacaagcc tggaaagtgg aacataaatt gtcggatatt ctactgttga
13561 ctatttgtgc cgttattttt ggtgcagaag gttgggaaat atagaggatt ttggggaaac
13621 acatctcgtt tttttgaagc aatatggtga ttttgaacat ggtattcctg ctcacgatac
13681 cattgccaga gttgtatcct gtatcagtcc tgcaaaattt cacgagtgct ttattaactg
13741 gatgcgtgat tgttattctt catatgataa agacgtcatc gcaattgatg gaaaaacact
13801 ccggcattct tatgacaaga gtcgccgcaa gggagcgatt catgtcatta gtgcgttctc
13861 aacaatgcac agtctggtca tcggacagat caagacggat gagaaattca atgaggtcac
13921 agctatccca gaacttctta acatatggat attaaaggaa aaattatcac aactgatgcg
13981 atgggttgcc agaaagatat tgcagagaag atacaaaaac gggggaggtg attatttatt
14041 cgctgtaaaa gtaaattagg ggcggcttaa taaagccatt gagggataat ccccgctgaa
14101 agaattaaat aatccaaagc atggcagtta cgaaataagt gaacagagtc acggcagaga
14161 agacatccgt cttcatattg tttgcgatgt tcctgataaa cttattgatt tcacgtttta
14221 atggaaagga ctgataaaat tatgcgtggc agtctccttt cggtcaataa tagcagaaca
14281 aaagaaggag cctgaaatga cggtcagata ttatatcagt tctgcgggtt taacagcaga
14341 gaagtttgcg acagtgattc gaaatcactg gcacgtgaaa aataagctgc actggcgtca
14401 gggcgtggta atgaatgaag accactgcaa aataagaaga ggaattgcag cagaattatt
14461 ttcagggatc aggtatatcg gcattaatat tttgacgaaa cataaagtat tcaaggctgg
14521 gttaagatgt aagatgagaa gagcagatat ggacagagac tacctcgcat cagtttttgc
14581 ggggagctgg ctttcgtaat cttgccctgg tgatgcaatg ctgaattaat agcattaaag
14641 attttaaata aaatggttta actcaacatg aactttataa ttattattag aatgattttg
14701 cctttctgta tttttttgag tattttactt tactctggaa gggtggatag tgttttttta
14761 ttttctattg aatacacatt tataagtgtg gcaatctttc tgataaagag aaagtttgta
14821 agtaatattt tatgggtaat ttatctttcc attttaggtg tgcaggcagc gtcgctattt
14881 agttcaggac aatatttgat gcctcttgcc ttgtcgaatg ctgcagaaat tgaatccttg
14941 ggtattggtg aggtaattaa agtttttttt gtttttattt tgtttattat aatttcattc
15001 attaccttgc cttcaggggc tctaaccgag cgtattaaat attataaatt caaccttctc
15061 tgtatagtat tattgctcgt gtgttctttc ataagtgcgg gaccagtata tgctttttat
15121 gaaacagcaa aattgttcta tttacagcaa acctttaagc ctagtaagaa gatcggagat
15181 gcagggaaat tctttttaaa aacaagtgtt tttattaacc ctatagaaaa caactacagt
15241 ttcaaaggcc gtaatgttat tgtaatattt gctgagggat tttcatctga aattatgggt
15301 aatgccaaac caagagcatt ttcagttacg cctaatttag ataaattatc tactgaagca
15361 ctaagtttca aaaattatta taaccataca gctgcaacat tccgaggatt aagaggtcag
15421 ttaacttctg gttatcaatt tcgagatggt ttaactgacg ctggcacagg tatcgcacaa
15481 ttatctaaag cagaaattaa taatatttac cttcaccgtc aaacatcttt acctgatata
15541 ttaaaagagt atggatataa aagttatttt atagcttcca ccgaaaaaaa tagcccactc
15601 aatacaatgt tgaagacact taattttgat aaggttcttg gtatgggtga ttttattggt
15661 tatcaacgag acagaatgac ggataagcaa acatttaatg cactgcaata ttttttacgt
15721 tctcaggaaa ataaaaaagg acccttcttt attggtgtct atccatctgg tactcatcat
15781 ggtcaagata gcccaaatga aaaatatttt gatggtaaca accctctcta caataagttc
15841 tataactatg attttcagtt agggaaattt attgacttct ttcggcgcag ttcattttat
15901 aataatacat tattaataat aacgtcagat cattcaacat ttccttcgac agaatataaa
15961 aaagcattta atagcgattc tcgttacttt gtaggcgaaa taccatttct ggttattgga
16021 ggagatatta ctcctgaaat tttggatgca gacggtaaaa attcattaag ctttgcgcca
16081 acaatattgc atatgttagg gattcagtat tcaatgaact attttttggg atgttctctt
16141 attgataagg aatgcacatc gccaganctg caggcatgca a
//