<pre>LOCUS       KP710588                9954 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain U 1-41 serotype O5:K4:H4 O antigen gene
            cluster, complete sequence.
ACCESSION   KP710588
VERSION     KP710588.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9954)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9954)
  AUTHORS   Baranzoni,G.M., Yan,X., Fratamico,P.M., Debroy,C., Tebbs,R.S.,
            Liu,Y., O'Connell,C.D., Swimley,M. and Matheny,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2015) Molecular Characterization of Foodborne
            Pathogens, United States Department of Agriculture, 600 E Mermaid
            Lane, Wyndmoor, PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="U 1-41"
                     /serotype="O5:K4:H4"
                     /db_xref="taxon:562"
     misc_feature    108..9736
                     /note="O antigen gene cluster"
     gene            108..1193
                     /gene="rmlB"
     CDS             108..1193
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="AJR19350.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDNVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPYPDEVNNKEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYIVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LAKMQWVENVKTGNYQSWIEQNYMERN"
     gene            1193..2056
                     /gene="rmlA"
     CDS             1193..2056
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase 2"
                     /protein_id="AJR19351.1"
                     /translation="MKGIILAGGSGTRLYPITMGVSKQLLPIYDKPMVYYPLSVLMLA
                     GIKDILIITTPEDQAGFIRLLGDGSQFGIEIKYATQSSPDGLAQAFIIGESFIGNDSV
                     CLVLGDNIFFGQGFTPKLKLACERTQGATVFGYQVMDPERFGVVEFDSQFKALSIEEK
                     PIKPKSNWAVTGLYFYDNDVIDIAKYIEPSSRGELEITTVNEVYLKNDSLNVELLGRG
                     FAWLDTGTHDSLIEAGSFVETVQKRQGMMVACLEEIAWRNGWLSKENLYERGQLLSKN
                     HYGQYLLKLVG"
     gene            2060..2455
                     /gene="fdtA"
     CDS             2060..2455
                     /gene="fdtA"
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-4-oxo-6-deoxy-alpha-D-glucose-3,
                     4-oxoisomerase'"
                     /protein_id="AJR19352.1"
                     /translation="MNIQLIPLQIHGDDRGALVSLERDKNIDFEIRRVYYIFDTKKGV
                     VRGYHAHKNLKQLVIAVKGSCRFTLDDGSERVSVFLDNPAQGLLVNSLIWREMSDFSE
                     DCVLMVLASNEYDESDYIRDYDAFKLAAK"
     gene            2452..2919
                     /gene="fdtC"
     CDS             2452..2919
                     /gene="fdtC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose
                     3-N-acetyltransferase"
                     /protein_id="AJR19353.1"
                     /translation="MMFIHKLSDVQSTSIGEGTNIWQFVVVLRKAIIGKDCNICANCF
                     IENDVVIGNSVTIKSGVFIWDGVRVHDNVFIGPCVAFTNDKYPRSKNYDAQFYETIIG
                     ENSSIGANSTILPGVKIGRNCMIGAGSVVTKDVPDNALVLGNPARIVKFLEAE"
     gene            2916..4019
                     /gene="fdtB"
     CDS             2916..4019
                     /gene="fdtB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose
                     transaminase"
                     /protein_id="AJR19354.1"
                     /translation="MIDFLNLQKSNARYNNELIEAAVRVIESGWYISGSELNAFENNF
                     AKYCKVKHAIGVANGLDALTLTLKAWQELGKLKRNDEVLVPANTYIASILAISEAGLK
                     PVLVEPNEFSYNLSVDNIRAKITANTRVILPVHLYGLISPMDEIMNFARENNLLVLED
                     SAQSHGASINGKKAGSWGDAAGFSFYPGKNLGALGDAGAVTTNDTDLADTVRYLRNYG
                     SKIKYKHEFKGVNSRLDEIQAAFLNVKLNYLDDDIRIRRNIANRYLNEIKNELIVLPT
                     ISSEENHVWHLFVIRIKDRDSLQHYLTKHNIQTVIHYPTPPHRQLAYKEFKDLCFPLS
                     EELHREVISIPMDPTLTEEQVSYVINVLNKFNC"
     gene            4022..5278
                     /gene="wzx"
     CDS             4022..5278
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AJR19355.1"
                     /translation="MNLIKVSFYNGISVIIKMLTMLGLNKVLAIYVGPSGYAIIGQYQ
                     SFVQTLATFSAGSISNGITKYTAQNVNVEKELIGVWKTAGTVSIVSSLFVSIMILIFF
                     KDINSVFFSNKIDNDLSYWLAASFVFMMLNSYLMAILNGMKQIKAYVLLNISSSILVL
                     VMTGLFAVTMGLKGALIALTINQSANFIITFSYFIKQKWFKIRFLAGNLNKTILKGLS
                     RFAFMALFSSLAVPLTQMFLRNFIGTNISWEISGYWEAINRISAINIMLATTTLTLYY
                     LPKLSETHEKKKYKHELINISKLLVPVTAFGCICVFYLKFYIINILFTPSFVNMSPLF
                     GWQMVGDFFKVFSWIFSFALLTKEKWKIFIACEILSAIVLAASTIIIVCFMSWHYLSA
                     AYAATYFIYLTFTSTFFYQSIYKKAS"
     gene            5279..6595
                     /gene="wzy"
     CDS             5279..6595
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AJR19356.1"
                     /translation="MEFTLNFIIIILSFIPGYYLGVFRFKRLNYYCVLYWSMLLFCVI
                     GSFIINSGVMDDSFFVGPIANNAEVKNLGLFITTISFFIFFLSAFIVEVFCSLYSGQS
                     SVMIHWNNYYSRPIIMSSYKVVLTLCILSCALVFYYIVSIYPAPLSMALQGASADEIA
                     VRRLEVTKNYSGIGYFKTLAGIIPIILSYYCFISYLKFKKHFLLFIFITCISTLTLAI
                     NGEKAPLVFYFLGLLVSYSSVREITKKIYVFSAFLIFSLILIMYVVLFQFDNSDYLLY
                     ILVERLFIAQEAAVFYAADYFSTHNLLGLSSMDTIFNKILSITPTARASEIFMYQYLP
                     GMAENGGWNVNGFFAHETFSNFGYIGIVLGSMYGGIVNGLLCIYFRAREKTVLTMSFY
                     SFYVVSVTTVLSSFNSMLFNTQLILVFIIFLILSVLDRRRKHAVPE"
     gene            6579..7457
                     /gene="wfgD"
     CDS             6579..7457
                     /gene="wfgD"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-Glc:alpha-D-GlcNAc-diphosphoundecaprenol
                     beta-1,3-glucosyltransferase"
                     /protein_id="AJR19357.1"
                     /translation="MQYQSDRVSIIIISYNHEQFIEKAIESVLFQTYKDIELIIVDNN
                     SSDNTREILNQYKDVYNIKVIYLENNTGITGGINEGLKYVSGEFVSFFASDDIMVSNR
                     IELQVNALRKEPEAIACFGNMLRINSDGSINKRGLLPMVGFKWWALNDILTNRICLYS
                     PTQLYRADVFTSDSINFPTEVRVEDIWLYFKLLNTGYKFITIPYLLTMYRVHDNNTHT
                     RYKMMMQEKIKILNDYKNEAFYKKALNFIYLEHFSNFGSTSKKDALRILPKVILNIKS
                     KYLYIGLIRLLFDWRK"
     CDS             7457..8125
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="AJR19358.1"
                     /translation="MFKHVYVDLCDTLIKGNTTFMFLDSFFSHHQNNRYWLYRKLSTS
                     FLMRVIFKLFFSAKIDLNRRIAIHFLNGYSIDFLREHVKWMLVNGIFIENKELVEVIQ
                     CAKSEHIPVTIVSASLDFIVEVVASHYSINYYSSKLAYKDGYCQGRIILDLLFNKNKI
                     FDDVEQNKSAYCFISDNIQDIQILKSCSYGFGVPNLKNEKAFIVNNIKLINYDRLINL
                     IKSE"
     gene            8128..8916
                     /gene="wbuO"
     CDS             8128..8916
                     /gene="wbuO"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuO"
                     /protein_id="AJR19359.1"
                     /translation="MKDYSIFYLPFWYSKQTRFRTIPRFFSWIIIYVIPVSLSFLFVS
                     PEFNYFNLFKSLLAILLVYNLYEIGYIYNDTETIKKEISPTLRLESHQLQFYENNKNN
                     IYSFRFLVALLITFALSFYDRVFIFLLASWAIIPAYALYNSVRSRLNIPLHFVLVTLR
                     YCSPVLLFTGGGGAYICFFMILLFPLINTLERCTESRFKFDYFKDLFLTNRKNGRYVY
                     YLILLIAGLTCCYIFGEYTFYVFSCYAFYYMAFRLLSAQVNLNE"
     gene            8909..9736
                     /gene="amseE"
     CDS             8909..9736
                     /gene="amseE"
                     /codon_start=1
                     /transl_table=11
                     /product="amylovoran biosynthesis protein"
                     /protein_id="AJR19360.1"
                     /translation="MNDSSRSFSVLMSLYYKEDPVYLDSCFASLANQLLLPTEIVCVF
                     DGPLNAALESVVFKWSEKLNITIVRLPVNVGLGNALNNGLAHCSFDIVARMDSDDICL
                     PERFQKQIPLFFSDKDLILLGSNIDEFEVCENTIKSVRKVPLSEAQIRECCKIKNPFN
                     HMTVVFRKNEILSLGGYQHHFLMEDYNLWLRVIAKGYKVCNIDSSLVKARVGNGMVGR
                     RKGIEYIKSEWKLFMLKKKLGFQSVFSGICIFLMRSVPRLLPKTVLSMLYSISRTKN"
ORIGIN      
        1 ctggtagctg taaagccagg ggcggtagcg tgcattaata cctctattaa tcaaactgag
       61 agccgcttat ttcacagcat gctctgaagt aatatggaat aaattaagtg aaaatacttg
      121 ttactggtgg cgcaggattt attggttctg ctgtagttcg tcacattata aataatacgc
      181 aggataatgt tgttaatgtc gataaattaa cgtacgccgg aaacctggaa tcacttgctg
      241 atgtttctga ttctgaacgc tatatttttg aacatgcgga tatttgcgat gctgctgcaa
      301 tggcacggat ttttgctcag catcagccgg atgcagtgat gcacctggct gctgaaagcc
      361 atgttgaccg ttcaattaca ggtcctgcgg catttattga aaccaatatt gttggtactt
      421 atgtcctttt agaagccgct cgcaattact ggtctgctct tgatagcgac aagaaaaata
      481 gtttccgttt tcatcatatt tctactgacg aagtctatgg tgatttacct tatcctgacg
      541 aagtaaataa taaagaagaa ttacccttat ttactgagac aacagcttac gcaccaagca
      601 gcccttattc cgcatcaaaa gcatccagcg atcatttagt ccgcgcgtgg aaacgtacct
      661 atggtttacc gaccattgtg actaactgct ctaacaatta tggtccttat catttcccgg
      721 aaaaattgat tccattggtt attctgaatg ctctggaagg taaagcatta cctatttatg
      781 gtaaagggga tcaaattcgc gattggttgt atgtcgagga tcatgctcga gcgttatata
      841 tagtcgttac tgaaggtaaa gcaggcgaaa cctataatat tggcggacac aacgaaaaga
      901 aaaacatcga tgtagtgctt actatttgtg atttgctgga tgagattgta ccgaaagaga
      961 aatcttatcg tgagcaaatt acttatgttg ccgaccgccc gggacatgat cgccgttatg
     1021 cgattgatgc tgagaagatt agccgcgaat tgggttggaa accacaggaa acgtttgaga
     1081 gcgggattcg gaagacagtg gaatggtatc tggcaaaaat gcagtgggtg gaaaacgtga
     1141 aaaccggtaa ttatcagtct tggattgaac agaactacat ggagcgtaac taatgaaggg
     1201 tataatttta gctggtggct caggtactag gttatatcca attacaatgg gtgtttcaaa
     1261 gcagttactc ccgatttatg ataagccgat ggtttattat ccactttcag tactgatgct
     1321 ggcaggtatc aaagatattc ttataattac cacaccagaa gatcaggctg gctttatccg
     1381 tttgctcggg gatggcagcc aatttggtat agaaatcaaa tatgcaactc aatcgagtcc
     1441 ggatggatta gcgcaggcat ttatcattgg cgaaagtttt attggcaatg attctgtttg
     1501 tcttgtatta ggtgataata ttttctttgg tcaaggattc actcccaaac ttaaacttgc
     1561 ctgtgaacgc acccaaggag cgactgtatt tggatatcag gtaatggatc ctgaaagatt
     1621 tggtgttgtc gagtttgata gccagtttaa agcattaagc atcgaagaaa aaccaataaa
     1681 accaaagtca aattgggctg ttactggttt atatttttat gacaacgatg taatagatat
     1741 tgctaaatat attgagcctt cttcacgagg tgaacttgaa attactactg tgaatgaagt
     1801 ttatttgaaa aatgattcgt taaatgtgga gttacttgga cgaggttttg cgtggcttga
     1861 cacagggaca cacgatagtt taattgaagc aggcagtttc gttgagacag ttcaaaagag
     1921 acaaggaatg atggttgctt gtctggaaga aatcgcctgg agaaatggtt ggctctcaaa
     1981 agaaaatctt tacgaaagag gccaactact gagtaaaaat cattacgggc agtatttatt
     2041 aaaattagta gggtgatata tgaatatcca acttatccca ttacagattc atggtgatga
     2101 tagaggggcc cttgtttctt tagaaagaga taagaatata gacttcgaaa ttagacgtgt
     2161 atattatata tttgatacaa aaaaaggggt cgtcagaggc tatcatgcgc acaaaaattt
     2221 gaaacagcta gttatcgctg ttaaaggatc gtgtcgtttt actttagatg atggaagcga
     2281 aagagtaagt gtgtttttag ataatcctgc tcaaggattg cttgttaatt ctttaatttg
     2341 gcgagagatg tcagatttct ctgaagattg cgtcttaatg gttttagcta gcaatgaata
     2401 tgacgaatct gattacatca gggactatga tgcgtttaaa ttggcggcaa aatgatgttt
     2461 atacacaaac taagtgacgt tcagtcaaca tctatcggag aaggtacaaa tatttggcaa
     2521 tttgtagtcg tccttagaaa agcaataatt ggtaaggatt gtaatatttg tgctaattgt
     2581 tttattgaaa atgatgttgt cattggaaat tctgtaacta taaaatcagg ggtttttatc
     2641 tgggatggag ttagagttca tgataatgtg tttattggtc cttgcgttgc atttactaat
     2701 gataaatatc cgcgttctaa aaattatgat gcacaatttt atgaaactat aattggtgaa
     2761 aattcatcaa taggggcaaa ttcgaccatt ctgcctggag taaagattgg gcgcaattgt
     2821 atgattgggg caggctctgt tgtaacgaaa gatgtgcctg ataatgcttt ggttttgggt
     2881 aatccagcaa ggatcgtgaa atttttggag gcagaatgat tgattttctt aacttacaga
     2941 aatccaatgc gcgttataat aatgaattaa ttgaagctgc agtaagagtt attgaaagtg
     3001 gatggtatat ctcaggctca gaactaaatg catttgagaa taattttgcg aagtactgta
     3061 aagttaagca tgccataggt gttgctaatg gacttgatgc tctgacgttg actcttaaag
     3121 catggcaaga gctaggtaaa ttaaagcgga atgatgaagt gcttgtgcct gcaaatactt
     3181 acatagcttc aattctagcc atcagtgagg caggtttaaa gcctgtttta gttgaaccaa
     3241 atgagttcag ctataatttg tcagttgata atatcagagc taaaatcaca gccaatacgc
     3301 gagttatact ccctgttcat ctttatggct tgatttctcc tatggacgaa attatgaatt
     3361 ttgccagaga aaacaatcta ttggtattgg aagatagcgc tcaatcgcat ggagcttcaa
     3421 tcaatggtaa aaaagcagga agttgggggg atgcagccgg gtttagcttt tatcctggca
     3481 agaatctagg tgcactaggt gatgcgggag ctgtaacgac taatgataca gacttagcag
     3541 atacggtacg ttatttgagg aattatggct caaaaataaa atacaaacat gagtttaaag
     3601 gtgtaaatag tcgattggat gaaattcagg cagctttcct aaatgtaaaa cttaattatt
     3661 tagatgatga tattcgtatt agacgtaata ttgcaaaccg atatttaaat gaaataaaaa
     3721 atgagttaat cgtacttcct actatttcca gtgaagagaa tcatgtatgg catctttttg
     3781 tcatcaggat aaaggatagg gattcgctgc agcattatct tacaaaacat aatattcaaa
     3841 cagtcattca ttatcctaca ccacctcatc gacaactggc atataaagaa ttcaaagact
     3901 tgtgtttccc tcttagcgaa gagttgcacc gtgaagtaat ttcaattcca atggatccca
     3961 cacttacaga agagcaagtt agctatgtca ttaatgtact gaataaattt aactgctaat
     4021 catgaattta attaaagtca gtttttataa tggtatttct gtaataatta aaatgctgac
     4081 tatgctagga cttaataaag tcctagctat ttatgtcggt cctagtggtt atgccataat
     4141 aggtcagtat cagagttttg ttcaaacact cgctacattt tcagcaggct caatatcaaa
     4201 tggtattaca aagtatacag ctcaaaatgt aaatgtggaa aaagaattaa taggtgtttg
     4261 gaaaactgct gggacagtca gtattgtttc cagtttgttt gtttcaatca tgattttgat
     4321 ctttttcaaa gatatcaatt ctgttttttt tagtaacaaa attgataatg atctttcata
     4381 ctggctcgct gcctcatttg tttttatgat gttgaatagt tatttaatgg ctattctaaa
     4441 tggaatgaag caaataaaag catatgtatt gcttaatata tccagtagca tattagtgtt
     4501 agtgatgact ggcctttttg cggttaccat gggtctgaaa ggtgctctca tagcattaac
     4561 aatcaatcag tcagcaaatt ttataataac cttctcatat tttattaagc aaaaatggtt
     4621 caagattcgt tttttagcgg ggaatttaaa taaaactatt ctcaaagggc ttagtcgatt
     4681 tgcttttatg gctttgttct catctttagc tgttccttta actcagatgt ttcttaggaa
     4741 tttcataggt acaaatattt cttgggagat atcaggttat tgggaagcca ttaatcggat
     4801 aagcgcaata aatattatgt tagcaaccac tacactaacc ctatattatt tgcccaagct
     4861 atctgaaaca catgagaaaa agaaatataa gcacgaatta atcaatatat ccaaattgtt
     4921 agtgccagtc acagcttttg gttgtatttg tgttttttat ttaaagtttt atatcataaa
     4981 tatacttttt actccttcat ttgttaacat gtcgccgctg tttggctggc aaatggttgg
     5041 agattttttc aaggtattta gctggatttt ttcttttgca ctcttgacga aagaaaaatg
     5101 gaagatattt atagcatgtg aaattttatc agcgattgtc cttgcagcat caacaatcat
     5161 aattgtttgt tttatgtcat ggcattatct gtctgcagca tatgcggcaa cgtactttat
     5221 atatctgaca tttacatcaa ctttcttcta tcagtccata tataaaaagg catcttagat
     5281 ggagtttact ttaaatttta ttataataat attgagtttt attcctgggt attacttggg
     5341 tgtgtttcgt tttaaaaggc tcaattatta ttgcgttctt tactggtcca tgctgctatt
     5401 ttgtgttatt ggatcattta taataaattc aggtgtcatg gatgattcgt tttttgtagg
     5461 accaattgca aataatgctg aggtaaaaaa ccttggttta tttattacta ctatttcttt
     5521 ctttatattc tttctgtctg cttttattgt tgaagttttt tgctcattat actcagggca
     5581 atcttccgta atgatacact ggaataatta ttactcgcgt cctataatta tgagtagtta
     5641 caaggtagta ttaacactct gtatattatc atgcgcattg gttttttatt acatagtatc
     5701 aatttatcct gcgccattgt ctatggcttt acagggagct tctgcggatg aaatagctgt
     5761 ccgacgatta gaagtgacaa agaattattc aggaattggg tatttcaaaa cattagcagg
     5821 tattatacct ataattcttt catattattg ttttatatca tatttgaaat ttaaaaaaca
     5881 ttttctactt tttattttta ttacttgtat ttcaacttta acattggcta taaacgggga
     5941 aaaagcacct ttggtttttt attttttagg gcttttagtt tcatattcat ctgtgaggga
     6001 aattactaaa aagatttatg tgttttccgc gtttttaata ttctccttga tactgataat
     6061 gtatgttgtt ctattccagt ttgataattc ggactatctt ctttatattc ttgtcgaaag
     6121 gttgtttata gcccaagagg ccgcagtatt ttatgcggca gattactttt ctacacataa
     6181 cttgctgggt ttatctagca tggatactat ttttaacaaa atacttagta ttactccaac
     6241 agcaagggct tcagagatat tcatgtacca gtatttgcca ggtatggcgg agaatggtgg
     6301 ttggaatgta aatggttttt ttgcacatga gactttctct aatttcggtt acattgggat
     6361 tgtgcttggc agtatgtacg gtggaatagt aaatggtttg ttatgcatct actttagagc
     6421 aagagaaaaa actgtgttaa caatgagttt ttatagtttt tacgttgtgt ccgtaacaac
     6481 tgtactaagt agttttaatt caatgctgtt taatacacaa ctgattttag tttttattat
     6541 ttttttgata ttatcagttc ttgacaggag aagaaaacat gcagtaccag agtgataggg
     6601 tctctatcat tataatatct tataaccatg aacaatttat agaaaaagca atcgaaagtg
     6661 tattatttca gacttataag gatatagagc ttattatcgt cgataataac tcttcggata
     6721 atactcgcga gatattaaac caatataaag atgtctataa cataaaagta atttatcttg
     6781 aaaacaatac gggtattaca ggtgggatta atgaagggtt aaaatatgtt agtggggagt
     6841 ttgtaagttt ctttgctagt gatgacatta tggtatcgaa tagaattgaa ctacaagtta
     6901 atgctttaag aaaagaacca gaggccattg cttgctttgg taatatgcta agaatcaaca
     6961 gtgatggctc aattaataaa cgaggtttgc taccaatggt cggttttaag tggtgggcac
     7021 tcaacgatat attgacaaac aggatatgct tatactctcc aacgcaattg tatagagctg
     7081 atgtttttac aagtgattca ataaacttcc caacagaagt cagggttgaa gatatctggc
     7141 tatattttaa acttctcaac acaggttata agtttattac tattccttat cttctgacta
     7201 tgtatcgagt tcatgataat aacactcata cgcgttataa aatgatgatg caagagaaaa
     7261 taaaaatctt aaatgattat aaaaatgaag ccttttataa aaaagcactc aattttatct
     7321 atcttgaaca tttctctaac tttggttcga cttccaaaaa agatgcgtta agaattctac
     7381 ctaaagttat attaaatatt aagtctaaat atttatacat cggtctcatc aggctacttt
     7441 ttgattggag aaagtaatgt ttaagcatgt atacgttgat ttgtgtgata ctttaataaa
     7501 aggcaacaca acgttcatgt tcttggattc cttttttagc catcaccaaa ataatcgcta
     7561 ttggctttac agaaaactca gcacttcatt tttaatgagg gtcatattta agttgttctt
     7621 ttcagctaaa atagatctaa atcgaagaat tgcgattcac tttttaaatg ggtacagcat
     7681 agattttctt agagaacatg taaaatggat gctagtcaat ggtattttca tagagaataa
     7741 ggaacttgtt gaagttatac aatgtgctaa aagtgaacat attccggtaa caattgtttc
     7801 ggcttcctta gatttcatag ttgaggttgt agcatcccat tattctatta attattacag
     7861 tagcaaactt gcatataaag atggatactg tcaggggcgt atcatccttg atcttctttt
     7921 taataagaat aaaatttttg atgatgtaga gcaaaacaaa agtgcttact gtttcatcag
     7981 tgataatatc caagatattc aaatattaaa atcttgtagc tatggttttg gtgttccaaa
     8041 cctgaagaat gaaaaagctt ttattgtaaa taatataaaa cttatcaact atgatagact
     8101 tattaacctg attaagagtg agtgattgtg aaagactata gtatctttta tcttccattc
     8161 tggtattcta agcaaactag gtttcgaaca attccaagat ttttttcatg gattataatt
     8221 tatgtgattc ctgtctcttt gtctttttta tttgtgtctc cagaatttaa ttattttaat
     8281 ttatttaaat cacttctggc tatcttatta gtctataatc tatatgagat tggatatata
     8341 tataatgata ccgaaacaat aaaaaaagaa atttcaccaa cactaaggct tgaatcccat
     8401 caattgcaat tttatgaaaa taataagaat aatatttatt cttttcgttt tttagttgca
     8461 ttattaataa cctttgcgtt atccttttat gatagggtat tcatcttttt gctggctagt
     8521 tgggctataa taccagcata tgcattatat aactcagtca gaagtagatt aaatatacca
     8581 ttgcattttg tattggtcac tttaagatac tgttcgccag tattattgtt tacgggggga
     8641 ggaggggcct atatttgttt ttttatgatt ttattattcc cccttataaa tacacttgaa
     8701 agatgtaccg agagtagatt taaatttgat tattttaaag acttgtttct taccaataga
     8761 aaaaatgggc gatacgtcta ttatcttatt ttgctcattg cgggacttac ttgttgttat
     8821 atttttggtg aatatacatt ctatgtgttt tcgtgttatg cattttacta tatggccttt
     8881 aggttattat ctgcgcaggt gaatcttaat gaatgatagt tcaagatcat ttagtgtatt
     8941 aatgtcttta tactacaaag aagatccagt ctaccttgac tcatgttttg caagtcttgc
     9001 caatcaatta ttacttccga cagaaattgt ttgtgttttt gatggtccac ttaatgctgc
     9061 attagaaagt gttgtattta aatggtctga aaaattgaat atcactattg tcaggctacc
     9121 ggtaaatgtt gggttaggta atgctttaaa taatggtctt gctcattgct catttgatat
     9181 cgttgcgaga atggattcag atgacatttg cctccctgaa cgttttcaaa aacaaatacc
     9241 tctttttttt agtgataaag accttatatt gctaggttct aacattgatg agtttgaagt
     9301 atgtgaaaat actattaaat cagtcaggaa agttccatta tccgaggctc aaattcgtga
     9361 gtgttgtaaa ataaagaacc cattcaatca tatgactgtg gtttttagaa aaaatgaaat
     9421 tttatcgctt ggtggttatc aacatcattt ccttatggag gattataacc tttggcttag
     9481 agttattgct aaaggatata aagtatgtaa tattgatagc tcattagtta aagctcgagt
     9541 gggcaatggg atggtgggga gaagaaaggg aattgaatat ataaaaagcg aatggaagct
     9601 ttttatgctc aagaaaaaac ttggatttca gtctgtcttt tccggaatat gcattttctt
     9661 gatgcgttct gttcctcgct tactccctaa aacagtgctc tcaatgcttt atagtatttc
     9721 aaggactaag aattaaacat ttcggttatt atccgtaaac agtatctctg agtaaatgaa
     9781 cgaaactgat gtttagccgt atttgttcgt tatcttaaat gacgacgcta ccaagctaaa
     9841 cactattgag ttaatatcaa tagcacagta aagccgctta ttagccgcgg taacccctga
     9901 caggagtaaa caatgtcaaa gcaacagatc ggcgtcgtcg gtatggcagt gatg
//

</pre>