<pre>LOCUS       KJ710508               11080 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain U 8-41 serotype O48:K-:H- O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ710508
VERSION     KJ710508.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11080)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11080)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Baranzoni,G.M.,
            Swimley,M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: 50.1
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="U 8-41"
                     /serotype="O48:K-:H-"
                     /db_xref="taxon:562"
     misc_feature    1..11080
                     /note="O-antigen gene cluster"
     gene            108..1193
                     /gene="rmlB"
     CDS             108..1193
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="AIG62375.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LANAKWVENVKSGAYQSWIEQNYEGRQ"
     gene            1193..2092
                     /gene="rmlD"
     CDS             1193..2092
                     /gene="rmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="AIG62376.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGVTKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKLEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTSAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            2150..3019
                     /gene="rmlA"
     CDS             2150..3019
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase 1"
                     /protein_id="AIG62377.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTSQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKALAEPL
                     KKKCLWSVSAEND"
     gene            3034..3594
                     /gene="rmlC"
     CDS             3034..3594
                     /gene="rmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="AIG62378.1"
                     /translation="MNVIKTDIPDVLIFEPKVFSDERGFFFESFNQKVFEEAVGRKIE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGIN
                     LSAENKRQLWIPEGFAHGFITLSDNAEFLYKTTNFYSPKHEVCIRFDDEKIGIEWQRA
                     EYMRLSEKDSKGVSLDEYVHMVVDRL"
     gene            3591..4505
                     /gene="epsE"
     CDS             3591..4505
                     /gene="epsE"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="AIG62379.1"
                     /translation="MNKVSILMPVYNVEKYIRAAITSILNQTYQNIELVIVDDASTDG
                     TSLIIDEIAKRNNNIIIVRNNVNSGITVALNKGLKYCSGDYIARADGDDIQDPLRIQK
                     QIDFLKENQEYGLVGCWIKNIDESGRQIASCKYPVSDLEVRQCVSYTSPVLHIWLAKK
                     DVYDKLNGYRNTNPAEDYDFILRCLDYGIRIGNVPYYGSMIRLRSGNTLTESSLKQRV
                     ISNYLCKKFKKGIINTIDIESELPKINEFNIITKIHSWSCFFLKKAIENKRTLKGAAY
                     LCLSLISPYTVQNIIKRQVFKRKYNKGY"
     gene            4604..5551
                     /gene="pglJ"
     CDS             4604..5551
                     /gene="pglJ"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylgalactosamine-N,
                     N'-diacetylbacillosaminyl-diphospho-undecaprenol
                     4-alpha-N-acetylgalactosaminyltransferase"
                     /protein_id="AIG62380.1"
                     /translation="MYLKVFYIKKELGLNFTCDTEVLTLGNVRKLYTFDVLHSHMLRP
                     DILVGLLPFYKGLKISTIHNIVTEDIQYTHGSVISKIVSELWFRIWNRFDKIVVLSKV
                     AKKYYTEHELKPKKLEVIYNGIDLCIGSNISSEDKEIIKKFKGNSTLLGTLCLANHRK
                     GIEQILYALPFLPNCKFIIIGDGPAREYLENLAISLNVRKRFFILGYRENAKDLLKYI
                     DIYLMPSRSEGFSLALLEAVAAKKTVVCSNIPIFKETFTERQVSFFDLDNTNSLIQAI
                     KNSINNDINDAYIRLKQNYSSDSMAQSYHELYMTCKEVK"
     gene            5548..6330
                     /gene="wbbL"
     CDS             5548..6330
                     /gene="wbbL"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="AIG62381.1"
                     /translation="MRVFISVVSHGHGELIESLRCLEKLSEKFSVVLKDNKNESNLKE
                     YCKHNNIIYLSSDIPKGFGENNNIIFNYCISELKMQSDDIFIIMNPDVIVEENELIKL
                     KNKMICENIEFATINLYKDRDYKIYDPSIRNYPGILDFVSGYLGFSNKTIINKSSINS
                     NTTVAWAAGSFLALKSYIYKEVGGFDIRYFMYCEDIDICMRINSYGYKLVYIPTIKAI
                     HIARHNNRRFLSKHFYWHLRSALIYLSEKRKYVSKKNKYHHK"
     gene            6296..7519
                     /gene="wzx"
     CDS             6296..7519
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AIG62382.1"
                     /translation="MSLKKISTTISKVIGGNALAQIIVILGTPLLTRIYQPSDFGIYS
                     TIMAVVLILGVIACGRYDQIMYNFDDKYSWYICFCNGLVIAICISLLCLITTGALFYF
                     FKFDISYLVIAPSIFTFAILQLYTSYFSLYSYYRTIIILNIIRTSSLVVLQLILFKFS
                     KNGLIVSFLLSQIICILFCLKLGRKLNRKFKFKVSFLKIREAGLSSVQSLASSFSSQL
                     PVLFISGQYGFYIVSLYGLAVRLTQVPIIFFTNAVRPYILGELNRNKRNYRLLSNIIW
                     RSSILLLSLSMIGIILINLFATDFFKIYAGEEWEGAGEIASALAWWLLVSFANVTSTS
                     YLTVIGRFRSLFIYDMFLLVFRVMAVCYSFYMKLSFIDFIYIYSIIGMLFNLGIIGYA
                     IKCSIRDAKNSDCYI"
     CDS             7729..8787
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferases family 1"
                     /protein_id="AIG62383.1"
                     /translation="MKVIHIVHNYYGFSGASLQAKNLAKALKKHAPNIEQIFVTRCQN
                     RISKLSEDVQFSVMEISSSYKRFWQFFLLCKSIKPDVVHFHGADFGLLGICAIFKIPV
                     YWKSTLLDCDDFHTLINRGSLRSLKHWLLRFISINNTLSKQIYEINSKYIPITRLVTI
                     PNGVSAPDCMYGNEQIKEKIVLIISAIIPRKKIIEGIKFYKDNFENQGYKLLIIGPDN
                     ESLDGFEAGYVLNCKQFSSNNIHFLGKIEHQKINEIMCRSQFLIHLSEREGMPNVVLE
                     ALANGVYPIIGEMGGLAYEMINNGSCGFICNNKDKFDINKYMGVNITGIDMVNEKYSF
                     DVISEKTFNIYMKLIGEK"
     gene            8784..9905
                     /gene="wzy"
     CDS             8784..9905
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AIG62384.1"
                     /translation="MKLENNNADKKISFIGLLWLISCGLYITQITAISPVYFIFILSM
                     ILVFLTNVSCRKWDVSFFTLISLIISLLSTFLFINSHFSLVINLCMVLLSPMLVDFYY
                     KGRSITKRNLLNVFLFYSFVFILDGFWRIQHPYLANVDKLEELGIGFQIYKVNSLMYA
                     DSNFVGLEAVFILSCFLYFFRDIKIVGLLRVRYFLVFCALFVGVVLTFSRAAIIAMIL
                     LFVLSVLMKNKRIKAFSYMLMPVLLIVLSWFVFKKFTDDISFESKFDLIRYSLEYIKN
                     NNVEVILLGVGLGNAVDVIGMGAHNLFLTFLIETGMIGLFLFLVLLCIMCIYLKEDAI
                     VIVLPFLIASMSLGTTAIPYFFTFTWLCILFKRKLFNVI"
     gene            9924..10934
                     /gene="galE"
     CDS             9924..10934
                     /gene="galE"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="AIG62385.1"
                     /translation="MNILVTGGLGYIGSHTLIRLIELKYNVIVVDNFSNSKASVIDRI
                     KALSGGEFVFYDYDICNAESLQEIFSKHDINKVIHFAGLKSVSESSRAPLTYYHNNVV
                     GTLNLLQLMCINGIKDIIFSSSATVYGLPDNMPLTEKCVTGGTTNPYGTSKHMVEQIL
                     MDLIQSDGDWNITILRYFNPIGAHPSGEIGEDPLGVPNNLVPYVTKVAIGDLPYLTIY
                     GDDYSTPDGTGIRDFIHVCDLAEGHIAALNNKKTNGLHIYNLGTGVGSSVLEIVNTFE
                     RVNNVSVPYRISKRRKGDVPECWADPSLIRAELSWSAKYNLEDMLKHAWLWQLKNPKG
                     YD"
ORIGIN      
        1 ctggtagctg taaagccagg ggcggtagcg tgcattaata cctctattaa tcaaactgag
       61 agccgcttat ttcacagcat gctctgaagt aatatggaat aaattaagtg aaaatacttg
      121 ttactggtgg cgcaggattt attggttctg ctgtagttcg tcacattata aataatactc
      181 aggatagtgt tgttaatgtc gataaattaa cgtacgccgg aaacctggaa tcacttgctg
      241 atgtttctga ctctgaacgc tatgtttttg aacatgcgga tatttgcgat gctgctgcaa
      301 tggcgcggat ttttgctcag catcagccgg atgcagtgat gcacttggct gctgaaagcc
      361 atgtggatcg ttcaattaca ggccctgcgg catttattga aaccaatatt gttggtactt
      421 atgtcctttt ggaagcggct cgcaattact ggtctgctct tgatggcgac aagaaaaata
      481 gcttccgttt tcatcatatt tctactgacg aagtttatgg cgatctgcct catcctgacg
      541 aagtaaataa taaagaagaa ttacccttat ttactgagac aacagcttac gcaccaagta
      601 gcccatattc tgcttcgaaa gcatcaagcg atcatttagt ccgcgcgtgg aaacgaacct
      661 atggtttacc gaccattgtg actaactgct ctaacaatta tggtccttat catttcccgg
      721 aaaaattgat tccattggtt attctgaatg ctctggaagg taaagcatta cctatttatg
      781 gtaaagggga tcaaattcgc gattggttgt atgtcgagga tcatgctcga gcgttatata
      841 cagtcgttac tgaaggtaaa gcgggtgaaa cttataacat tggtggacac aacgaaaaga
      901 aaaacatcga tgtagtgctc actatttgtg atttgttgga tgagattgta ccgaaagaga
      961 aatcttaccg cgagcaaatt acttatgttg ccgatcgccc gggacacgat cgccgttatg
     1021 cgattgatgc agagaagatt agccgcgaat tgggctggaa accgcaggaa acgtttgaga
     1081 gtgggattcg taaaacggtg gaatggtacc tggctaatgc aaaatgggtc gagaatgtga
     1141 aaagtggtgc ctatcaatcg tggattgaac agaactatga gggccgccag taatgaatat
     1201 tctccttttc ggcaaaacag ggcaggtagg ttgggaacta cagcgtgctc tggcaccttt
     1261 gggtaatttg attgctcttg atgttcactc cactgattat tgtggtgatt ttagtaatcc
     1321 tgaaggtgtg gctgaaaccg tcaaaaaaat tcgccctgat gttattgtta atgctgcggc
     1381 tcacaccgca gtagataagg ctgagtcaga acccgaattt gcacaattac tcaatgcgac
     1441 tagcgttgaa tcaattgcaa aagcggctaa tgaagttggg gcttgggtaa ttcattactc
     1501 aactgactac gtattccctg gaaatggcga cacgccatgg ctggagacgg atgcaaccgc
     1561 accgctaaat gtttacggtg taaccaagtt agctggagaa aaagcattac aagagcattg
     1621 tgcgaagcac cttattttcc ggaccagctg ggtctatgca ggtaaaggaa ataacttcgc
     1681 caaaacgatg ttacgtctgg caaaagagcg tgaagaatta gcggttatta acgatcagtt
     1741 tggtgcacca acaggtgctg agctgctggc tgattgtacg gcgcatgcca ttcgtgtggc
     1801 attgaataag ctagaagtcg caggtttgta ccatttggta gccagtggta ccacaacctg
     1861 gcacgattat gctgcgcttg tttttgaaga ggcgcgcaaa gcaggcattc cccttgcgct
     1921 caacaagctc aacgcagtac caacatcagc ctatcctaca ccagctcgtc gtccacataa
     1981 ctctcgcctt aatacagaaa aatttcagca gaactttgcg cttgtcttgc ctgactggca
     2041 ggttggcgtg aaacgaatgc tcaacgaatt gtttacgact acagcaattt aatagttttt
     2101 gcatcttgtt cgtgatgatg gagcaagatg aattaaaagg aatgatgaaa tgaaaacgcg
     2161 taaaggtatt attttagcgg gtggttctgg tacacgtctt tatcctgtga ctatggctgt
     2221 cagtaaacag ctattaccta tttatgataa gccgatgatc tattacccgc tctctacact
     2281 gatgttggcg ggtattcgcg atattctgat tattagtacg tcacaggata ctcctcgttt
     2341 tcaacaactg ctgggtgacg gtagccagtg ggggctaaat cttcagtaca aagtgcaacc
     2401 gactccagat gggcttgcgc aggcgtttat tatcggtgaa gagtttatcg gtggtgatga
     2461 ttgtgctttg gttcttggtg ataatatctt ctacggccac gatctgccga agttaatgga
     2521 tgtcgctgtt aacaaagaaa gtggtgcaac agtatttgct tatcacgtaa atgatcctga
     2581 acgctacggt gtcgttgagt ttgataaaaa cggtacggca attagtctgg aagaaaaacc
     2641 gttacaacca aaaagtaatt atgcggtaac tgggctttat ttttatgata acgacgttgt
     2701 cgaaatggcg aaaaatctta agccttctgc ccgtggtgaa ctggaaatta ccgatattaa
     2761 ccgtatttat atggaacagg ggcgtttatc cgttgccatg atggggcgtg gttatgcatg
     2821 gcttgacacg gggacgcatc aaagtcttat tgaagcaagc aacttcattg ccaccattga
     2881 agagcgccag ggactaaagg tttcctgccc ggaagaaatt gcttaccgta aagggtttat
     2941 tgatgcagag caggtgaaag cattagctga gccgctgaaa aaaaaatgct tatggtcagt
     3001 atctgctgaa aatgattaaa ggttagtaat aaaatgaacg taattaaaac tgacattcca
     3061 gatgtgctaa tttttgaacc gaaagttttt agcgatgagc gcggtttctt ttttgagagt
     3121 tttaaccaga aggtttttga ggaagctgtc gggcggaaga tcgagtttgt tcaggacaac
     3181 cattcgaagt caagcaaagg tgttttacgc gggctacatt atcagctaga accttacgca
     3241 caaggaaaat tggtgcgttg tgttgttggt gaagtttttg atgtggcggt tgatatccgt
     3301 aaatcttcac ccacttttgg taaatgggta gggattaatt tatctgctga aaataaacgg
     3361 caattgtgga tcccggaagg gtttgcacat ggattcataa cgttgtcaga taatgcagaa
     3421 tttctttata aaacaactaa tttttattct ccaaaacatg aagtttgtat tcgatttgat
     3481 gatgaaaaaa tagggattga gtggcagcgt gcagagtata tgagactctc agaaaaagat
     3541 tcaaaagggg tatctctcga tgaatacgtg catatggtgg ttgatagatt atgaataaag
     3601 ttagcatatt aatgcctgtt tataatgtgg aaaaatatat tagggctgcg attactagta
     3661 ttttaaacca aacatatcag aatatagaat tagtgattgt agacgatgcg tctactgatg
     3721 gtacctcgct cattatagat gaaattgcga aaagaaataa taatataatt attgttcgta
     3781 ataatgtaaa ctcagggata actgtagcac taaacaaggg gcttaaatat tgtagcggtg
     3841 attatatagc tagagctgat ggtgatgata tacaagatcc attacgaatt caaaagcaaa
     3901 tagattttct aaaggagaat caagaatatg gattggtcgg atgttggata aaaaacatag
     3961 atgagtcagg cagacaaata gcttcatgta aatatccggt ttcagactta gaagtgagac
     4021 aatgtgttag ctatacatct cctgtacttc atatatggtt agcaaagaaa gatgtttacg
     4081 ataaacttaa tggatacagg aatacaaatc cagctgaaga ttatgatttt attttaagat
     4141 gtttggatta tggtataaga atagggaatg ttccgtatta tggatcaatg atacgtttgc
     4201 gttcggggaa tacactaact gaatcttctc taaaacaaag agttatatca aactatctgt
     4261 gcaaaaaatt taaaaaaggc atcattaata caattgatat tgagtctgag ttacctaaaa
     4321 taaatgaatt taacatcatc actaaaatac attcttggtc atgtttcttt ttaaaaaaag
     4381 caatagagaa taaaagaaca ttaaaaggtg cagcttattt atgtttgtcg cttataagtc
     4441 cttatacagt tcaaaatata ataaagcggc aggtttttaa aagaaagtat aacaagggtt
     4501 actaagttga taaaaatagc aataatatta ccatcattga aaaaacgttg gaccgaatag
     4561 agttgcacat actcttatta atagattaag cgataaagaa ggtgtgtacc ttaaggtttt
     4621 ctatataaaa aaagaattag gattaaattt tacttgtgat acagaagttt tgactttagg
     4681 taatgtgcga aaattataca cctttgatgt tttgcattca catatgctta ggccagatat
     4741 cttggttggg ttacttccat tttataaagg acttaaaata agtacaatcc ataatatagt
     4801 aacagaagat atacaatata cacatgggag cgtgatatct aaaattgtct ctgaattatg
     4861 gtttaggatt tggaatagat ttgataagat agtcgtgtta tcaaaagtag caaaaaaata
     4921 ttatacggaa catgaactaa aacctaagaa attagaggtg atatataatg gaattgatct
     4981 gtgtatagga agtaatatta gttctgaaga taaggaaata ataaagaagt ttaaaggaaa
     5041 cagtactctt ctgggaacac tttgcttagc aaatcacagg aaaggtatag aacagattct
     5101 gtatgccttg ccattcttac caaattgtaa atttataatt attggtgatg ggcctgcaag
     5161 agaatattta gagaatttag ctataagtct gaatgtgaga aagcgatttt ttattctggg
     5221 ctatagagaa aacgctaaag atttactaaa gtatatcgat atttatttaa tgccaagtag
     5281 atcggaagga tttagtctcg cattgcttga ggctgttgct gcaaaaaaaa cagtagtttg
     5341 ctcaaatatt ccaatattta aagaaacctt tactgaacga caagtgtctt tttttgacct
     5401 ggataataca aactcactta ttcaagcgat aaagaattca ataaataatg atataaatga
     5461 cgcgtatata aggctaaagc aaaattatag ttcagattct atggctcaat catatcatga
     5521 attatatatg acttgtaagg aagtaaaatg agggttttta tatctgttgt atcacatgga
     5581 cacggggaat taattgagtc attaaggtgt ttagaaaaac tttcggaaaa gtttagtgtt
     5641 gtgcttaaag ataataaaaa tgaatccaat cttaaagagt attgcaagca taataatata
     5701 atctatttaa gtagcgatat tcctaaaggc tttggtgaga ataataatat tatatttaat
     5761 tattgcatat ctgaattgaa aatgcagtca gatgatatat ttataataat gaatccagat
     5821 gtaattgttg aagaaaatga attgataaaa cttaaaaata agatgatatg tgaaaatata
     5881 gagtttgcta ctataaatct ttataaagat agagactata aaatatatga tccatcaatc
     5941 aggaattatc cgggcatcct tgactttgta agtggttatc ttggtttctc aaataaaaca
     6001 ataataaaca aatcgtcaat taattcaaat actaccgtag cttgggccgc agggtcgttc
     6061 ttggcattaa agtcatatat ctataaagaa gtaggtggtt ttgatatccg atatttcatg
     6121 tattgtgaag atatagatat atgcatgagg ataaattctt atggctataa gttagtttac
     6181 ataccaacta taaaagccat acatattgcg aggcacaata atcggcgttt tttatcaaaa
     6241 catttttatt ggcatttgcg tagtgcactt atatatttat cggaaaagag aaagtatgtc
     6301 tctaaaaaaa ataagtacca ccataagtaa agtaatcggt ggtaatgcat tagcacaaat
     6361 tattgttata ttaggcaccc ccttacttac aagaatttat caaccaagtg attttggtat
     6421 atattctact atcatggcag tggttttaat attgggagtt attgcgtgcg gtagatatga
     6481 tcagataatg tataatttcg atgacaaata ttcgtggtac atatgttttt gtaatggact
     6541 tgttatcgca atatgcatat ccttattgtg tttaattacg actggagcat tattttattt
     6601 ttttaaattt gatataagtt accttgttat tgctccatca atatttacct ttgcgatatt
     6661 gcagttatat acgtcttact ttagtctgta ttcttattac agaaccataa taatattaaa
     6721 tatcattagg acttcaagct tagttgtatt acaattaatt ttgtttaagt tttctaaaaa
     6781 tgggcttatt gtgagctttc tattatctca aataatatgt atcctatttt gcttaaaact
     6841 aggaagaaaa cttaatagaa aatttaagtt taaagtgagt tttttgaaga tcagagaagc
     6901 cggtttgagc tctgttcagt ctcttgctag ttctttttct tcacaattgc cagttctttt
     6961 tatttctgga caatatggtt tttatattgt aagtctgtat ggcttggctg taagattaac
     7021 tcaggtacca attatttttt tcacaaatgc cgttagacca tatattctgg gagagttaaa
     7081 taggaataaa agaaattatc gtcttttatc aaatataata tggagaagct cgatcttatt
     7141 actatctcta agtatgatcg gaataattct cattaattta tttgcaactg atttttttaa
     7201 aatttatgca ggtgaagaat gggaaggcgc tggtgaaata gcttcagctt tagcatggtg
     7261 gctcttggta tcttttgcta atgttacttc aacttcatat ttaactgtta ttggacgatt
     7321 cagatcttta tttatctatg atatgttcct attggtattt agagtaatgg ctgtatgcta
     7381 tagcttttat atgaagttat catttataga tttcatatac atttattcta taattggtat
     7441 gcttttcaac ttgggtatta tcggttatgc gataaaatgt agcattcgag atgctaaaaa
     7501 tagcgattgt tatatttgag tacgcttgct ttgtgtaagg tgaattcact tgaggtaaag
     7561 gtgtattgaa gaccaaaaaa ctgacggtaa taaaatatta ttaacgttat ttcaatggtt
     7621 ttaccttttt tgtgtgttgt gtgatagcaa aaattttatt atagacagca caatagtaaa
     7681 taaatttaaa atgttttcta ttaggttttc aaagagaaaa atatataaat gaaagtgatc
     7741 catatagtac ataattatta tggctttagc ggcgcatctc ttcaagcaaa aaatttagca
     7801 aaggctttaa aaaaacatgc ccccaatatc gaacaaattt ttgtgactag atgccagaat
     7861 cgtatttcta aattaagtga ggatgttcaa ttcagtgtaa tggaaatatc ttcatcatat
     7921 aaaagatttt ggcagttctt tcttttgtgt aaatctatta aaccggatgt tgttcatttc
     7981 catggggctg attttggtct tcttggtatt tgtgctattt tcaaaatacc tgtctattgg
     8041 aaatcaactt tattggattg tgatgatttc catacattga taaatcgtgg ttctttgagg
     8101 tctcttaagc actggttgtt gaggtttatt tcgataaaca atacgttatc gaaacaaata
     8161 tatgaaatta atagtaaata cattcccatc actaggttgg ttactattcc aaatggcgtc
     8221 agtgcccctg attgtatgta cgggaatgaa cagattaagg aaaaaatagt tttaataata
     8281 tctgcaatta taccaagaaa aaaaataata gaaggaataa aattttataa agataatttt
     8341 gaaaaccaag gatataaact tttgataatt ggccccgata atgaaagtct tgatggtttt
     8401 gaagcaggtt atgtgctgaa ctgtaagcag tttagtagta ataatatcca ttttttaggt
     8461 aaaattgaac atcaaaaaat taatgaaata atgtgtcgtt ctcaattcct aatacattta
     8521 tcggagcgag aaggtatgcc aaatgttgtg ttggaggcac ttgctaatgg tgtctatccg
     8581 ataattgggg aaatgggagg gcttgcttat gaaatgataa ataacggttc ttgtggattt
     8641 atttgcaata ataaagataa gtttgatata aataaatata tgggtgttaa tataactgga
     8701 atagatatgg tcaacgaaaa atattcgttt gatgtgattt cagaaaagac atttaatata
     8761 tatatgaaac ttattggtga aaaatgaaac ttgaaaataa taatgctgat aaaaaaatct
     8821 catttatcgg ccttttatgg ttgatatctt gtggtttata tataacccaa ataactgcga
     8881 ttagtcctgt ttattttatt tttattcttt caatgatttt ggtttttctt acaaatgtta
     8941 gttgcaggaa atgggatgtt tcttttttca cactcataag tcttattata tcactattgt
     9001 caactttcct ttttataaac tcgcattttt cacttgtaat taatctgtgt atggtgctgc
     9061 tttctccaat gttggtggat ttttattata agggacgaag cattacaaaa agaaatttat
     9121 tgaatgtttt cttgttttat tcattcgttt tcattcttga tggtttttgg agaattcaac
     9181 atccgtatct tgcaaatgtt gataagttag aagaactggg tatcggcttt caaatatata
     9241 aagtaaactc acttatgtat gccgattcca actttgttgg tctagaagct gtttttatat
     9301 tatcctgttt tctttatttc ttcagagata ttaaaattgt tggtttattg cgcgtaagat
     9361 actttcttgt tttttgtgct ttatttgttg gggttgtttt aactttctct agggctgcta
     9421 tcatagcaat gatattgctt tttgtgcttt ctgttctaat gaaaaataag cgcatcaaag
     9481 ctttctcata tatgcttatg ccagtattac ttattgttct ttcatggttt gtatttaaaa
     9541 aattcacgga tgatataagc tttgaaagta aatttgattt aattaggtat tcactcgaat
     9601 acattaagaa taataatgtt gaggtgatat tgctgggggt tggtcttgga aatgcagtcg
     9661 atgttattgg tatgggggca cataatctgt ttctgacttt cttaattgaa actggcatga
     9721 ttggattatt tttattttta gtattattat gcattatgtg catttacctc aaagaggatg
     9781 ctattgtcat cgtgcttcca tttttgatag catcaatgtc actcggaaca actgcaattc
     9841 cttacttttt tacctttact tggttatgta ttctttttaa aagaaaatta tttaatgtca
     9901 tttgacattg taaaggaaac aacatgaaca tattagttac tggtggctta ggatatatag
     9961 gttctcatac tttaattcga ttgattgagt taaaatataa cgtcatagtt gttgataatt
    10021 tttcaaactc taaagcaagt gtgattgaca gaataaaagc gctgagcggt ggagaatttg
    10081 tattttatga ttatgatatt tgtaatgctg aatctctgca agaaattttt tctaaacatg
    10141 atattaataa ggttattcat tttgctggat tgaaatcagt ttcagaatct agtagggcac
    10201 ctttaacata ttatcacaac aatgtagttg gaacgttaaa cttattgcag ttaatgtgta
    10261 taaatgggat aaaggatatt atatttagtt cttctgcgac tgtatatgga ctgccagata
    10321 atatgccatt aactgaaaaa tgtgtaactg gaggaactac aaatccttac gggaccagta
    10381 agcatatggt cgaacaaatt ttgatggatt taattcaatc tgatggcgat tggaatataa
    10441 ctattcttag atattttaac cctattggtg cccacccttc aggagagatt ggagaggatc
    10501 ctctaggagt tcctaataat ctagtacctt acgtgactaa agtcgcaatt ggggatttgc
    10561 cgtatttgac catttatggc gatgattata gtacgcctga tggaactgga atcagagatt
    10621 ttattcatgt ttgcgattta gctgaaggac atatagctgc attgaataat aaaaaaacaa
    10681 atggtctaca tatttataat ttaggtacgg gtgttggttc atctgtatta gagatagtaa
    10741 atacatttga gcgagttaat aatgtctctg ttccttatcg tatctccaaa cgacgaaagg
    10801 gtgatgtgcc tgaatgctgg gccgatccat cattgattag agctgaatta agttggagcg
    10861 caaaatataa tcttgaggac atgttgaagc atgcttggct ttggcaactc aaaaatccaa
    10921 agggttatga ttaatcgagg caacactttt attggttcat tatttatgat aataaaggtg
    10981 tatgtttaca tcattttaaa taccaccatc aaagcggcaa tcctgacagg agtaaacaat
    11041 gtcaaagcaa cagatcggcg tcgtcggtat ggcagtgatg
//

</pre>