<pre>LOCUS       FJ940774               14060 bp    DNA     linear   BCT 01-MAY-2010
DEFINITION  Escherichia coli serogroup O35 O-antigen gene cluster, complete
            sequence.
ACCESSION   FJ940774
VERSION     FJ940774.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14060)
  AUTHORS   Liu,B., Wu,F., Li,D., Beutin,L., Chen,M., Cao,B. and Wang,L.
  TITLE     Development of a serogroup-specific DNA microarray for
            identification of Escherichia coli strains associated with bovine
            septicemia and diarrhea
  JOURNAL   Vet. Microbiol. 142 (3-4), 373-378 (2010)
   PUBMED   19932572
REFERENCE   2  (bases 1 to 14060)
  AUTHORS   Liu,B., Wu,F., Li,D., Beutin,L., Chen,M., Cao,B.Y. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-2009) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..14060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O35"
     misc_feature    1..14060
                     /note="O-antigen gene cluster"
     gene            745..1830
                     /gene="rmlB"
     CDS             745..1830
                     /gene="rmlB"
                     /note="dTDP-D-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ACV67279.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDVAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAVRNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSSSKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KGLPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            1830..2729
                     /gene="rmlD"
     CDS             1830..2729
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ACV67280.1"
                     /translation="MNILLFGKIGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPKFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQEEDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GIHNLHDYAALVFEEARKAGIPLALNKLSAVPTTAYPTPARRPHNSRLNTEKYQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            2787..3665
                     /gene="rmlA"
     CDS             2787..3665
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ACV67281.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFING
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKKGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCLEEIAYRKGFIDAEQVNVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            3668..4213
                     /gene="rmlC"
     CDS             3668..4213
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-glucose-3,5-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ACV67282.1"
                     /translation="MNVIKTEIPDVLILEPKVFGDERGFFMESFNQKVFDEAVGRKVE
                     FVQDNHSKSIKGVLRGLHYQQEPYAQGKLVRCVVGEVFDVAVDIRRDSETFGKWVGVN
                     LSAENKKQLWIPEGFAHGFYVLSDTAEFVYKATNYYNFLSDRGIIWNDKNININWPIV
                     GDILLSEKDMNHRTFTETFNV"
     gene            4244..5518
                     /gene="gna"
     CDS             4244..5518
                     /gene="gna"
                     /note="UDP-glucose-6-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gna"
                     /protein_id="ACV67283.1"
                     /translation="MKLENLNIGIVGLGYVGLPLAVEFGKKFVTVGFDIKRARVEELK
                     NNIDSTYECSSNELQLANLLKFTNNIDDIRKCNVYIVTVPTPIDKFKRPDLSPLINAS
                     KLIGSVLNKGDVVIYESTVYPGATEEECVPVLEEQSGMIFNKDFFVGYSPERINPGDK
                     EHRVTSIKKVTSGSTIEIANFVDSLYATIINAGTYKASSIKVAEAAKVIENTQRDLNI
                     ALINELAIIFNKLNIDTEEVLKAAGTKWNFLSFKPGLVGGHCIGVDPYYLTHKAQSIG
                     YNPEVILSGRRINDAMGEYVASQLVKKMIKKKIKIDCADVLIMGLAFKENCPDLRNTK
                     VIDIIKSLRDYNINAEVYDPWVSPDEAAQEYGVNINNKVPPKKYDAILFAVAHNEFKD
                     MTKEEILSLTKNNYVIYDLKYIIASDLVIDRL"
     gene            5543..6565
                     /gene="gne"
     CDS             5543..6565
                     /gene="gne"
                     /note="UDP-GlcNAc-4-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gne"
                     /protein_id="ACV67284.1"
                     /translation="MNYEELQDYLLNNQRTWLITGVAGFIGSNLLEKLLNLNQCVIGV
                     DNFSTGFQSNLNEVKDNVPESSWRRFKFIEGDICNLDVCKKSISGVDYVLHQAALGSV
                     PRSIENPILTNASNISGFLNVLDCARRENVKSFTYAASSSTYGDHTGLPKVENIIGNP
                     LSPYAVTKYVNELYAGVYALNYNFKSIGLRYFNVFGRRQNPEGAYAAVIPKWILSILN
                     GDDLYINGDGNTSRDFCYIDNVVQANLLAALANDNVKNNIFNIAYGQQTSLNKLFKYI
                     TVALSNEGVEYKKQPIFKEFRLGDVRHSLANIDKAKIMLGYEPCYNTNQGIKLAIKWY
                     INNLTK"
     gene            6678..7883
                     /gene="wzx"
     CDS             6678..7883
                     /gene="wzx"
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ACV67285.1"
                     /translation="MYLKILIDNISLVIQYFFGGIAVFYVTPLIVKSVGIHTYGNLAI
                     MFAIVTYISVVIQYSFNLIGPKLIAEGNCKKTFNTIISAKIVLFLLSLIFFLLYFYFF
                     QNKHDDVFLLFLVFPLSWVFNSAWYLQSKGYFVISSVCSIIGSLITFVIAYFFINTTT
                     GLFVPILCLVLSSFINGLLTFFFAVKNNGHIEIVNPLPMLKEGMPLFVSQIISSLYTM
                     SGVFIITYFYGVASAGTYAIVERFMNLLISLGVLTHVAAYPKLARLFNKDRLEYRKTL
                     LFVIALYTLFSCCVAIVVFNFHQNIVTYMFGSESVNAKELIYSACIFIFVSIYGPVVT
                     GYFTLKVKGRMIITINIIIALLSLLLGVGMLKIVGSSGWLIGLSLAQLLYIAIFFKIF
                     IWGRKECAV"
     gene            7871..9715
                     /gene="wbpS"
     CDS             7871..9715
                     /gene="wbpS"
                     /note="aminotransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbpS"
                     /protein_id="ACV67286.1"
                     /translation="MCGLVGFFSKANHDTNIIRNMLHKIRHRGPDSFGIWADLECNIH
                     FGHVRLSIVELSSAGHQPMSTSCGRFTIIFNGEIYNHLDIRRELGNNIKWSGTSDTET
                     LLKSISTWGISSTLKKMVGMFSFALWDSVERSLYLARDRMGEKPLYYGWCNGSFIFGS
                     ELKALKSHPDFDAEIDWQAINGYLHNNYISSPLTIYSKLKQLRPGHYIKMSYDDLLSG
                     NIPVLYKYWALSFPVSDNNCRYVDSVSELETLLTESVSLQSIADVKVGAFLSGGIDST
                     TIVAMLKKSGRDVSTFSIGMPNKQFDESHHAEQIAKYIGTQHYTHMITPQEALEVIDN
                     IPAIWDEPFADSSQIPTYLVSKFAKEYVTVALSGDGGDELFMGYNQYPLLKRIWDTRF
                     LSNLHLEFIANIMTKMGFKNSNVIVKRALNLSQGWRCKTPFLLNDFWMDKYRNAEFPL
                     LKPIRCERDLDLNYSDGISAITQHDLNYYLCDDILTKVDRASMAVSLETRAPFLDHRV
                     VEFAFALPTSFKLDKYNQKKILKSVLYKHVDSKLLERPKQGFSLPMKYWLKAELKGWA
                     RDRLDSLPDDVFDKVVVDNIWKDHINDIKDNSERIWGLSNLANFLELQ"
     gene            9712..10461
                     /gene="wcnX"
     CDS             9712..10461
                     /gene="wcnX"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcnX"
                     /protein_id="ACV67287.1"
                     /translation="MKKIAIYTCVTGGYDVVKAPLKINHNIDYICFSDQKISAPYPWK
                     VRNIAELKISKSFDKKTINRAIKICPQDFGLLEEYELTIYIDGSIEIMDDLSLLIDFV
                     TKQDYDIFMYEHFLRNCLYDEAEECLLIGYDWYWNIQKQVKRYKQRGFPVSYGLFECG
                     IIIRKKSRDLNVILQKWFEEYVKGVKRDQLSLTYILWENGYHLYSLGESDARYKNRHF
                     KLHRHSIKSNEYLRKFRSKLNKLLLIFWGGI"
     gene            10463..11329
                     /gene="wcnY"
     CDS             10463..11329
                     /gene="wcnY"
                     /note="rhamnosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcnY"
                     /protein_id="ACV67288.1"
                     /translation="MYDKVCGIVIVFYHPNDENINSAKKLSESYKVIIVDNSEKDITY
                     SIPKAHIIKLKRNVGIAAALNIGIHFFIKNNYKYAVLLDQDSEPDKSLLSSLINYSEN
                     CTDNVCLVAPSYYDRAINKNADFILCTEKGIIRQPAIGKNAIEASYVITSGSLLRLSS
                     ISNIGFMDEDLFIDFVDIEWCLRANSLGYKILGLPWLKMSHEIGDKPIKILNKKYVNH
                     SPIRHYYYFRNIFLLMRMSHIHPQWKKWELIKLLPRFFVYAFFTKNNMKHIVSMLTGV
                     YDGILGRVGKKK"
     gene            11326..12348
                     /gene="wzy"
     CDS             11326..12348
                     /gene="wzy"
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ACV67289.1"
                     /translation="MNGVAKPIIISWFVLCTLVVFFLCSFEQFPDYYSYLNWYELSVS
                     ATLNNNWVFFKDPGFYLLSVISNNFDFGIIGAIFFLFIISLSCKIFFCIKLLDWEVLF
                     WVLLLYLSRLFIIHDLVQYRAGASIGLSALFVYFYLEKKRIKSFFFLSLALSIHLSSL
                     LMVSVIPIAWFLNRNVGGNIIRQIAILLLILSLSLFFDPYVNLLRLVSQFPLMHERIA
                     PYLDGSYLVSNTSMFNSFVIIKIISYVIFFVWICKNKNVGLTNENYLIYLCFFISIVG
                     LFLFWCFRSNDSLSIRFSDFFALYDIVFFALLLNVFDVFGKFIYRYCLLVVVIVFFIS
                     SMKLIN"
     gene            12370..13155
                     /gene="wcnZ"
     CDS             12370..13155
                     /gene="wcnZ"
                     /note="rhamnosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcnZ"
                     /protein_id="ACV67290.1"
                     /translation="MNVYISVVSHNHAKMIMEFDCLRKLAKRYKVVIKNNSERESVIL
                     NDYCRENGIYIIDYAYNTGFGKNNNIVFQFCVKKLGMTSDDYFILINPDVIIDSINID
                     KLISIIEKDMVDISGISLYKDDSLSIRDYSIRNYPSLYTFFISFFRFHESYRVLPPND
                     DCSSVDMDWVAGSFMAFKTNAYSSLLGFDENYFMYCEDLDICFRAKSKGLNLKYIPSV
                     TGIHKAQHNNRRLFSKHFYWHIKSALRFLVRKKIKLQVKSILK"
ORIGIN      
        1 tctgtcatcc ggaccaaaga gccgctggac cgcgaaggta aagtcagccg cattgttgaa
       61 tttatcgaaa aaccggatca gccgcagacg ctggactccg acatcatggc cgttggtcgc
      121 tatgtgcttt ctgtcgatat ttggccggaa cttgaacgca cacagcctgg tgcatgggga
      181 cgtattcagc tgactgatgc cattgctgaa ctggcgaaaa aacagtccgt tgatgccatg
      241 ctgatgactg gtgacagcta cgactgcggt aaaaaaatgg gttatatgca ggcgtttgtg
      301 aagtatggac tacgcaacct caaagaaggg gcgaagttcc gcaaaggtat tgagaagctg
      361 ttaagcgaat aatgaaaatc tgactggatg taacggttga taagaaaatt ataacggcag
      421 tgaagattcg tggcgaaagt aatttgttgc gaattttcct gccgttgttt tatataaaca
      481 atcagaataa caacgagtta gcaataggat tttcgtcaaa gttttccagg attttccttg
      541 tttccagagc ggattggtaa gacaattagc gtttgagttt ttcgggttta gcgcgagtgg
      601 gtaacgctcg tcacatcgta gacatgcatg cagtgctctg gtagctgtaa agccaggggc
      661 ggtagcgtgc attaatacct ctattaatca aactgagagc cgcttatttc acagcatgct
      721 ctgaagtaat atggaataaa ttaagtgaaa atacttgtta ctggtggcgc aggatttatt
      781 ggttctgctg tagttcgtca cattataaat aatacgcagg atagtgttgt taatgttgat
      841 aaattaacgt acgccggaaa cctggaatca cttgcagatg tttctgattc cgaacgctat
      901 gtttttgaac atgcggatat ttgtgatgta gctgcaatgg cacggatttt tgctcagcat
      961 cagccggatg cagtgatgca cctggcagct gaaagccatg ttgaccgttc aattacaggc
     1021 cctgcggcat ttattgaaac caatattgtt ggtacttatg tccttttaga agcggttcgg
     1081 aattactggt ctgctcttga tggcgacaag aaaaatagct tccgttttca tcatatttct
     1141 actgacgaag tctatggtga tttgcctcat ccagatgaag taaataatac agaagaatta
     1201 cccttattta ctgagacaac agcttacgca ccaagcagcc cttattcttc atcaaaagcg
     1261 tccagcgatc atttagtccg tgcgtggaaa cgtacctatg gtttaccgac cattgtgact
     1321 aattgctcta acaattatgg tccttatcat ttcccggaaa aattgattcc attggttatt
     1381 ctcaatgctc tggaaggtaa aggattacct atttatggta aaggggatca aattcgcgac
     1441 tggctgtatg ttgaagatca tgcgcgtgcg ttatataccg tcgtaaccga aggtaaagcg
     1501 ggtgaaactt ataacattgg tgggcacaac gaaaagaaaa acatcgatgt agtgctcact
     1561 atttgtgatt tgttggatga gattgtcccg aaagagaaat cttaccgcga gcaaattact
     1621 tatgttgccg atcgtccggg acacgatcgc cgttatgcga ttgatgctga gaagattggt
     1681 cgcgaattgg gatggaaacc acaggaaacg tttgagagcg ggattcgtaa aacggtggaa
     1741 tggtacctgt ccaatacaaa atgggttgat aatgtgaaaa gtggtgccta tcaatcgtgg
     1801 attgaacaga actatgaggg ccgccagtaa tgaatatcct cctttttggc aaaatagggc
     1861 aggtaggttg ggaactacag cgtgctctgg cacctctggg taatttgatt gctcttgatg
     1921 ttcactccac tgactactgt ggtgatttta gtaatcctga aggtgtagct gaaaccgtaa
     1981 gaagcattcg gcctgatatt attgtcaacg cagccgctca taccgcagta gacaaagcag
     2041 aatcagaacc gaagtttgca caattactga acgcgacgag tgtcgaagcg atcgcgaaag
     2101 cagccaatga agtcggcgcc tgggttattc actactctac tgactacgta tttccgggaa
     2161 ccggtgaaat accatggcag gaggaagatg caaccgcacc gctaaatgtt tacggtgaaa
     2221 ccaagttagc aggagaaaaa gcattacaag agcattgtgc gaagcacctt attttccgga
     2281 ccagctgggt ctatgcaggt aaaggaaata acttcgccaa aacaatgttg cgtctggcaa
     2341 aagagcgtga agaattagcc gttattaatg atcagtttgg tgcgccaact ggcgcagagt
     2401 tgctggctga ttgtacggca catgccattc gtgtggcact gaataaaccg gaagttgcag
     2461 gcttgtacca tctggttgct agtggtatcc acaacctgca cgattatgct gcgctggtat
     2521 ttgaagaggc gcgcaaagca ggcattcccc ttgcactcaa caagctcagc gcagtaccaa
     2581 caacagccta tcctacacca gctcgtcgtc cacataactc tcgccttaat acagaaaaat
     2641 atcagcagaa ctttgcgctt gtcttgcctg actggcaggt tggcgtgaaa cgaatgctta
     2701 acgaattatt tacgactaca gcaatttaat agtttttgca tcttgttcgt gatggtggag
     2761 caagatgaat taaaaggaat gatcaaatga aaacgcgtaa aggtattatt ttagcgggtg
     2821 gttctggtac tcgtctttat cctgtgacta tggccgtcag taaacagctg ttaccgattt
     2881 atgataaacc gatgatctat tacccgcttt ctacactgat gttagcgggt attcgcgata
     2941 ttctaattat aagtacgcca caggatactc ctcgttttca acaactgctg ggtgacggga
     3001 gccagtgggg gctaaatctt cagtacaaag tgcaaccgag tccagatggt cttgcgcagg
     3061 catttatcat cggtgaagag tttatcaatg gtgatgattg tgctttggtt ctaggtgata
     3121 atatctttta cggtcacgat ctgccgaagt taatggatgt cgctgttaac aaagaaagtg
     3181 gtgcaacggt atttgcctat cacgttaatg atcctgaacg ctacggtgtc gttgagtttg
     3241 ataaaaaagg tacggcaatt agcttggaag aaaaaccgtt acaaccaaaa agtaattatg
     3301 cggtaaccgg gctttatttc tatgataacg acgttgtcga aatggcgaaa aaccttaagc
     3361 cttctgcccg tggtgaactg gaaattaccg atattaaccg catttatatg gaacaggggc
     3421 gtttatccgt tgccatgatg ggacgtggtt atgcatggct ggacacgggg acacatcaga
     3481 gcctgattga ggcaagcaac tttattgcaa caattgaaga gcgccaaggg ttaaaggtat
     3541 cttgcctgga agagattgct tatcgtaaag gctttattga cgcagagcag gttaatgtat
     3601 tagccgaacc actaaagaaa aatgcttatg gtcagtatct gctaaaaatg attaaaggtt
     3661 actaaaaatg aatgtaatta aaactgaaat tcctgatgta ttaattttgg agccgaaagt
     3721 ttttggtgat gagcgcggtt tttttatgga aagctttaat cagaaagttt tcgacgaggc
     3781 tgtagggcgt aaggttgaat ttgttcagga taaccattcc aaatcaatta agggggtgtt
     3841 acgcggactg cactatcagc aggaacctta tgctcaaggt aaattagttc gttgtgtggt
     3901 tggagaggtc tttgatgttg cggtggacat ccgtagagac tctgaaacat ttggtaaatg
     3961 ggttggtgta aatctttcgg ctgaaaataa aaaacaatta tggatacctg aaggttttgc
     4021 tcatgggttt tatgtattga gtgatactgc tgaatttgtc tataaagcga ctaattatta
     4081 taattttcta tcagatcggg ggatcatttg gaatgataaa aatataaata tcaactggcc
     4141 aattgtcgga gatatacttc tttctgaaaa agatatgaat cataggactt ttactgaaac
     4201 atttaatgtt tgatattgaa acttacattt agagagataa ttaatgaaac tagaaaattt
     4261 aaatattggc atagtcggtt taggttacgt cggtttaccg cttgcggtcg agtttggtaa
     4321 aaagtttgtg acagttggtt ttgatataaa aagagcgaga gttgaagaac taaaaaataa
     4381 tattgattca acttatgaat gctcaagcaa tgaactacag ttggctaatt tattaaaatt
     4441 cacaaataac attgatgata ttaggaaatg taatgtatat attgtaactg taccaactcc
     4501 aatagataag tttaaacggc ctgatttatc accattaatt aatgcatcaa aattaatagg
     4561 ttcagtattg aataaaggtg atgttgttat atatgagtca acagtatatc cgggggcaac
     4621 tgaagaagaa tgtgttcctg tactagaaga acaatcaggt atgattttta ataaggattt
     4681 ttttgtagga tatagtcctg agagaattaa tcctggtgat aaagaacatc gtgttacttc
     4741 aataaagaaa gttacatctg ggtcgaccat tgaaattgcc aattttgtag attcattata
     4801 tgcaaccata attaatgctg ggacttataa agcaagttca ataaaagtag cagaagcggc
     4861 gaaagtaatt gagaatactc aacgtgattt aaatattgca ctgattaatg aattggctat
     4921 tatatttaat aagttaaata ttgatacaga agaggtacta aaagctgcag ggactaaatg
     4981 gaactttttg tcatttaaac caggacttgt tggtgggcat tgtattggag ttgatcccta
     5041 ttacttgaca cataaagctc aatccatcgg atataatccg gaagttatat tatcaggaag
     5101 aagaattaac gatgctatgg gggaatatgt ggcgtcacag ttagtaaaaa aaatgataaa
     5161 aaagaaaatt aaaatcgatt gtgcagatgt cttaattatg gggttagcat tcaaagaaaa
     5221 ctgtcctgat ctaagaaata ctaaagtaat agatattata aagtctttaa gagattataa
     5281 tatcaatgca gaggtttatg atccttgggt ttccccagat gaagctgccc aagaatatgg
     5341 tgtcaatatt aataataaag tcccgcccaa aaaatatgat gctattttgt ttgccgttgc
     5401 tcataatgaa tttaaagata tgacgaaaga ggaaattctc tcattaacaa aaaataatta
     5461 tgttatatac gatctaaagt acattatagc gtctgacttg gttattgatc gcttgtaata
     5521 tataggcttt gaggacgatt acatgaatta tgaagagtta caagactatc tgttaaataa
     5581 tcaaagaact tggttaatta ctggtgtggc tgggtttatt ggctccaact tacttgaaaa
     5641 acttttaaat ctaaatcaat gcgttattgg cgtggataat ttttcaactg gttttcaatc
     5701 aaatttaaat gaagttaaag ataatgtacc tgaatcttct tggagaagat ttaaatttat
     5761 tgaaggagat atctgtaatt tagatgtctg caaaaaaagt atcagtggtg tcgattatgt
     5821 tctgcaccaa gcagcacttg gttcagtacc gagatcaatt gagaatccca ttctgaccaa
     5881 tgcttcaaat attagtggtt ttttaaatgt tttagattgt gcaagaagag aaaatgtaaa
     5941 aagttttact tacgctgcta gtagttcgac atatggtgat catacaggtt tacctaaagt
     6001 tgaaaatatt ataggtaatc cactttctcc ttatgctgtg acaaaatatg taaatgagtt
     6061 atatgcaggt gtttatgcac taaattataa tttcaaaagt ataggtctta gatattttaa
     6121 tgtatttggg agaaggcaaa atcctgaagg ggcatatgcg gctgtgattc ctaaatggat
     6181 cctttcgata ttgaatggtg atgatctata tataaatggt gatggaaata cgagtagaga
     6241 tttttgttat atagataacg tagttcaagc taatctatta gcagcacttg caaatgacaa
     6301 cgtaaagaat aacatattta atattgctta tggacaacaa acaagtctta ataaactttt
     6361 taaatatatt acagttgctt taagtaatga aggagtcgaa tacaaaaaac aacctatatt
     6421 taaggaattt cgattaggag atgtaagaca ttcgctcgct aatattgata aagctaaaat
     6481 tatgttggga tatgaaccat gctataacac taaccaggga ataaagttag ctattaagtg
     6541 gtatataaat aatctgacca aatgaaacat agtggctgta ggctcgagat taaagtgagt
     6601 ctgcagttac tataatgcat caggattgaa atatatccat gccatttatt ggaatttaat
     6661 atgcacaaag aattcacatg tatttaaaaa tattaataga caatatttca cttgtaatcc
     6721 aatatttttt tggtggcata gcggtgttct atgttacacc gttaattgtg aaatctgttg
     6781 gtattcatac ctatggtaat ttagcaatta tgtttgctat tgttacttac atatcagtcg
     6841 taatacagta ttcatttaat ttgattgggc caaaattaat tgctgaagga aattgcaaaa
     6901 aaacatttaa tacaataata tccgctaaaa tagtactttt tttattgtca cttatttttt
     6961 ttctattata tttttatttc tttcaaaaca aacatgatga tgtattttta ttatttcttg
     7021 tttttccact ttcttgggtt tttaatagcg cttggtatct tcaaagtaaa ggttattttg
     7081 ttataagtag tgtgtgctca ataattggat cactaatcac atttgttatc gcgtattttt
     7141 ttataaatac aacaactggc ttatttgttc caatattatg tttggtttta tcttcgttta
     7201 taaatggcct tcttactttt ttctttgcag taaaaaataa tggacatatt gagatagtta
     7261 atccactacc tatgttgaaa gaaggaatgc ctctttttgt ttcccagata atctcctcgt
     7321 tatatacaat gtctggtgtt tttataatta cctattttta tggagttgca tctgcaggta
     7381 catatgccat tgtagaacgt tttatgaact tattaatttc tttaggcgtt cttacacatg
     7441 tggcagccta tccaaaattg gcgaggttat tcaataaaga tagattagaa tatagaaaga
     7501 cattattatt tgttattgca ttatatactt tgttttcttg ttgtgtagcc atagttgttt
     7561 ttaactttca tcaaaacatt gtaacatata tgtttgggag cgaaagtgta aatgctaaag
     7621 aactaattta ctcagcgtgt atatttatat ttgtttccat atatggaccg gtagtgacag
     7681 ggtattttac tttaaaagta aaagggagaa tgataataac aattaatata ataatagctt
     7741 tgctatcttt actgctagga gtagggatgt taaaaatagt gggtagtagt ggatggttaa
     7801 taggactgag tctcgctcag ttactttata ttgctatctt ttttaagatt tttatttggg
     7861 gaagaaaaga atgtgcggtc tagttggttt ttttagtaaa gctaatcatg atacgaatat
     7921 aattcggaat atgttgcata aaattagaca tcgtggaccc gattcattcg ggatttgggc
     7981 cgatttagaa tgcaatatcc attttggcca cgttagatta tcgattgttg aattgagtag
     8041 cgcaggacat caaccgatgt caacttcatg tggacgattt actattatat ttaatggtga
     8101 gatatataac cacctcgata tacgtcggga acttggcaat aatataaagt ggtcaggaac
     8161 atctgatact gaaaccttac ttaaaagtat ttcgacttgg gggatttcta gcacattgaa
     8221 aaaaatggtt gggatgtttt cctttgcatt gtgggatagt gtagaacgaa gtttatatct
     8281 tgcgagagat agaatgggtg aaaaacctct ctattacggt tggtgtaatg gttcttttat
     8341 atttggctcg gaactgaaag cattaaaatc acatcctgat tttgatgctg aaattgactg
     8401 gcaggcaatt aatggatatt tgcataataa ttatatttct tcacccttaa ctatttatag
     8461 caagttaaaa caattgcgac ctggtcatta cattaaaatg agctatgatg atcttctctc
     8521 aggtaatata ccggtgctgt ataagtattg ggcattatct tttcctgtta gtgataataa
     8581 ctgcaggtat gttgattctg tgtcagaatt agagacttta ctaacagaat ctgtttctct
     8641 tcagtcgatt gctgatgtta aggttggagc ctttctatca ggtggaatag attctactac
     8701 catcgtagca atgttgaaaa aaagtggacg tgatgtatct acattttcta tcggaatgcc
     8761 taataaacaa tttgatgaat cacaccacgc cgaacagatc gcaaaatata ttggtactca
     8821 gcattataca catatgataa cacctcaaga agctcttgag gtaattgata atattcctgc
     8881 aatttgggat gagcctttcg cagatagttc tcagatacca acatacttgg ttagtaagtt
     8941 tgctaaagag tatgtaacag tagctttgtc tggtgatggt ggagacgagc tgttcatggg
     9001 atataaccaa taccctttgt taaagagaat ttgggacact agatttttat ctaatctgca
     9061 tttagaattt atagctaata taatgacaaa aatgggattt aaaaattcca atgtaattgt
     9121 aaaaagggct ttgaatttaa gtcaaggttg gcgttgcaaa actccttttt tattaaatga
     9181 tttttggatg gataagtatc gcaatgcaga atttccttta ttaaaaccta tacgttgtga
     9241 acgtgattta gatttaaatt actcagatgg aatttcagca ataactcagc acgatttaaa
     9301 ttattatctt tgtgatgata tattaactaa agtagacaga gcctcaatgg cggttagttt
     9361 agaaacgcga gctccatttt tagatcatcg cgttgttgaa tttgcgtttg ctttgccgac
     9421 ctcattcaaa ctggataagt ataatcagaa aaaaatactc aagtcagtat tatataaaca
     9481 tgttgatagt aaattattag aacgccctaa gcaagggttt tcattaccta tgaagtattg
     9541 gttaaaagct gagctaaaag gatgggcacg agatcgtctt gattctttac cagatgatgt
     9601 tttcgataaa gttgttgttg ataatatttg gaaagatcat attaatgata tcaaggataa
     9661 tagcgagcga atttggggtt tgagtaattt agctaatttt ttggagttac aatgaaaaag
     9721 attgctatat atacttgtgt taccggcggt tatgatgtag ttaaagcccc acttaaaatt
     9781 aatcataata tagattatat atgtttcagc gatcaaaaaa tttcagctcc ttatccttgg
     9841 aaagttagaa atatagcaga gcttaaaata tcgaagtcat ttgacaagaa aacaattaat
     9901 cgcgctatca aaatatgtcc tcaagatttt ggtctattag aagaatatga actaactatt
     9961 tatatagatg gctcgataga aattatggac gatctgtctt tactaattga ttttgtaaca
    10021 aaacaagatt acgatatttt tatgtatgaa cattttttaa gaaattgcct gtatgatgaa
    10081 gctgaagagt gtcttctaat tggatatgat tggtattgga atattcaaaa gcaagttaaa
    10141 agatataagc agcgagggtt tccagtttca tatggtctct ttgagtgtgg gattattata
    10201 agaaaaaaat ctcgagactt aaatgtaata ttacaaaaat ggtttgaaga atatgtaaaa
    10261 ggagttaagc gtgaccagct ttcgctcacg tatatattat gggaaaatgg ttaccatttg
    10321 tattccttgg gggagagtga tgctagatat aaaaatagac attttaagtt acataggcat
    10381 tcaataaaaa gtaatgaata tctcaggaag tttaggtcta aacttaataa gttgttgtta
    10441 attttttggg gagggatata aagtgtacga taaagtttgt ggtattgtaa ttgtatttta
    10501 tcatcctaat gatgaaaata ttaatagtgc aaaaaagtta agtgagtctt ataaagtaat
    10561 aatagttgat aatagtgaaa aggatataac ttattccata cctaaagcgc atattataaa
    10621 attgaaaaga aacgtaggta tcgctgcagc actaaatatt ggaatacatt tttttattaa
    10681 aaataattat aaatatgctg ttttattaga tcaagatagt gaacctgata aatcactttt
    10741 gagttcactt atcaattatt ctgaaaattg cacggataat gtatgtttag tagcaccgtc
    10801 ctattatgat agagctatta ataagaatgc tgattttatt ctatgcactg aaaaaggtat
    10861 tattagacag cctgcaattg gaaaaaatgc gattgaagcg tcctatgtta taacttctgg
    10921 ttctctctta agactgtcat ctatttctaa tattggattc atggatgaag atttatttat
    10981 agattttgtt gatattgagt ggtgtttaag agcaaattct ttgggatata aaatattagg
    11041 tttgccatgg ttaaagatgt cacatgagat cggtgataaa cctattaaaa tattaaataa
    11101 aaagtatgtt aatcattccc caattagaca ctattactat tttagaaata tatttctgtt
    11161 gatgcgtatg agtcatatcc atccacaatg gaaaaaatgg gagttgataa agttattacc
    11221 tagatttttt gtttatgcat tttttacaaa gaataatatg aaacatattg tctctatgtt
    11281 aactggtgta tatgatggta ttcttgggcg tgtggggaaa aagaaatgaa tggagtagcc
    11341 aaacccatta taatttcttg gtttgttctt tgtactcttg tggttttctt tttatgtagt
    11401 ttcgaacaat tcccggatta ttattcatat ctcaactggt atgagttatc agtatctgca
    11461 acactgaata ataattgggt cttttttaaa gatcccggat tttatttgtt atcagttata
    11521 tcaaataatt ttgattttgg tatcattggg gcaatttttt tcctttttat tatatcactt
    11581 tcatgtaaga ttttcttttg tattaagtta cttgattggg aagtattatt ctgggtttta
    11641 cttttatatc tttccagact ttttattatt catgatcttg ttcaatatcg tgcaggtgct
    11701 tcaattggtt tatcggcatt atttgtatac ttttatttag aaaaaaaaag gattaagtct
    11761 ttttttttct taagtctcgc actttctatc cacctttcaa gtttgttaat ggtgtcagtc
    11821 atccctattg catggtttct taataggaat gtaggtggaa atataattcg acagatagca
    11881 atattacttt taatattgtc gttgagctta ttttttgatc cttacgttaa cttgctcaga
    11941 ttggtatcac agtttccctt aatgcatgaa agaattgctc cgtatttgga tggttcttat
    12001 cttgttagta acacatcaat gtttaatagt tttgtaataa ttaaaattat ttcatatgtg
    12061 attttttttg tttggatatg caaaaacaaa aatgtcgggt tgactaatga aaattactta
    12121 atttatcttt gtttcttcat atctattgta ggattgtttc ttttttggtg ttttagaagc
    12181 aatgactcac tttccatacg tttttctgat tttttcgctt tgtatgatat cgtttttttt
    12241 gctctattgt taaatgtttt tgatgtgttt ggaaaattta tctatagata ttgtctttta
    12301 gtcgtggtga ttgttttttt tatatcttca atgaagttaa taaattaatt tcttatatgt
    12361 gtgctaacta tgaatgttta tatatctgtt gtctctcata atcatgctaa aatgattatg
    12421 gaatttgatt gtttacgcaa acttgctaag aggtataaag tagttattaa aaataattct
    12481 gaacgcgaaa gcgttatttt gaatgattac tgtagggaaa atggaatata tataattgat
    12541 tatgcctata atactgggtt tggcaaaaat aataatattg tatttcaatt ttgtgtaaaa
    12601 aaattaggga tgactagtga tgattatttt atccttataa atcccgatgt tataattgat
    12661 tctattaaca ttgataaact tatttccata attgaaaagg atatggtaga tatttctgga
    12721 atatctctgt ataaagatga ttctttgtcg ataagagatt attcaatacg taattatcct
    12781 tcgctttaca ctttttttat atcgttcttc cgattccatg aaagttatcg tgtattgcca
    12841 cctaatgacg attgctcaag tgtagatatg gactgggtgg ctggttcttt tatggctttt
    12901 aaaacaaatg catattccag tttattaggt ttcgatgaaa attacttcat gtattgcgag
    12961 gatttagata tttgttttag agccaagagt aagggcttaa atttaaagta catacccagt
    13021 gttactggaa ttcataaggc tcagcataat aatagaagat tatttagcaa gcatttttat
    13081 tggcatataa aaagcgcact aagattttta gttagaaaaa aaataaaatt acaagtgaaa
    13141 tctatattga aatgatatct taatatcagt ggagttttaa agatgtcata atatatatcg
    13201 tttgctatct atttatatta gttttaggtt agaatctatt actagataac cgcgcatatt
    13261 ttccgcggtg accacaccag acaggagtaa acaatgtcaa agcaacagat cggcgtcgtc
    13321 ggtatggcag tgatggggcg caaccttgcg ctcaacatcg aaagccgtgg ttataccgtc
    13381 tctattttca accgttcccg tgagaagacg gaagaagtga ttgccgaaaa tccaggcaag
    13441 aaattggttc cttactttac ggtgaaagag tttgttgaat ctctggaaac gcctcgtcgc
    13501 atcctgttaa tggtgaaagc aggtgcaggc acggatgctg ctattgattc tctcaagccg
    13561 tacctcgata aaggtgacat catcattgat ggtggtaaca ccttcttcct ggacaccatt
    13621 cgtcgtaacc gtgagctttc ttcagaaggc tttaacttca tcggtaccgg tgtctccggt
    13681 ggtgaagaag gtgcgctgaa aggtccttcc attatgcctg gtgggcagaa agaagcctat
    13741 gaactggttg cgccgatcct gaccaatatc gccgccgttg ctgaagatgg cgaaccgtgt
    13801 gttacctata ttggtgccga tggtgcgggt cactatgtga aaatggttca caacggtatt
    13861 gaatacggtg atatgcaact gattgctgaa gcctattctc tcctgaaagg cggcctgaat
    13921 ctctctaacg aagaactggc acagaccttt accgagtgga ataacggtga actgagcagc
    13981 tacctgatcg acatcaccaa ggatatcttc accaaaaaag atgaagacgg taactatctg
    14041 gttgatgtga tcctggatga
//

</pre>