LOCUS       AB811610               15355 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli DNA, O-antigen biosynthetic locus, strain: P2a.
ACCESSION   AB811610
VERSION     AB811610.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 15355)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..15355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="P2a"
                     /db_xref="taxon:562"
                     /note="serogroup:O30"
     gene            <1..902
                     /gene="wcaM"
     CDS             <1..902
                     /gene="wcaM"
                     /codon_start=3
                     /transl_table=11
                     /protein_id="BAQ00777.1"
                     /translation="AILRQGFHNQIIXANITNCKFSDLQGDAIEWNVAINDSDILISD
                     HVIERINCTNGKINWGIGIGLAGSSYDNNYPEDQAVKNFVVANITGSDCRQLIHVENG
                     KHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGIIKG
                     KYLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPK
                     YLFMRNINVMQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVD
                     IDRINHHIVNVEKINFRLPERRE"
     gene            1065..1976
                     /gene="galF"
     CDS             1065..1976
                     /gene="galF"
                     /note="O030_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ00778.1"
                     /translation="MDEIMTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYI
                     VDEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVT
                     IMNVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNE
                     TGRSQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGR
                     YVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQA
                     FVKYGLRNLKEGAKFRKGIEKLLMRSN"
     CDS             2259..3416
                     /note="O030_02"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ00779.1"
                     /translation="MKVLHIARNVPIKSLRGNPVILDLINRLSQAGIVNKLAFPAEYI
                     PSFSFLRGRPRIFSELKGEFNIGGLEVLFVNFIRIPGRWSFLFSRFYFNKKKLDKFSE
                     GVNCIHAHYIIPDGLVAYELAKNKNIPYIVTIRQGDLDRINKLKHGDCSYKQYEKVVS
                     NAAFLLAINPNIANEIKRIFQKNCITIPHGVDKSLIEYGIENRIHKKIIVAAQFIKRK
                     NIDWVIKVFNSMTDQGISNYTLTIIGEGELENDLKKLASGNDKIIFKPWLSKKELMGE
                     FLLSDIFIMPSDQETFGMVYIEAAAKNCLIIGKKDTGVDGYFENEKNALFISTVGELE
                     IVIKKILLGLIDIREMQNLGTILVEKNMTWDKVTSNYIKLYHKAIANVQNQ"
     gene            3400..4626
                     /gene="wzy"
     CDS             3400..4626
                     /gene="wzy"
                     /note="O030_03"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="BAQ00780.1"
                     /translation="MFRISDFFKVNLLFLILTFSYVNLISKQFSYMGFKDDFNIERFG
                     VSLIFCVLVAIYYASVQEFFNKFISTLFLIFVIMPNLVLFSGMGGDIRIIIWCVLSLP
                     ITLFLLRILPVIKLPVIKDKQVGALLWGLLILCIIPVLLAHGINFNLKVFLLDVYDIR
                     RESRLANTMASVYAYFWLAKVICPIALVYAIERKKIVMAMIFTLVLLYLFMTTGHKSV
                     FFTVILIMVMYKGGMDYQKKSNYIFNGALILFIFSILLTAIFSINILESLFIRRLLFI
                     PALLNTYYFDFFDNKFVYYSSSYLSSIIDYPYDRPIPEVIGLNYFNSDEMSANNGYIS
                     DGFANAGSIGIFINIVLASILLKIFKDYDVNPKYAGLIFVSFYAIQGSAMSTVLMTHG
                     GILLLILVPMVLGRRK"
     gene            4623..5828
                     /gene="wzx"
     CDS             4623..5828
                     /gene="wzx"
                     /note="O030_04"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="BAQ00781.1"
                     /translation="MIKKILKLLSGPALGHLITIALTPYFMYHYSPSIFGIYAFFTAI
                     LGVFVSISTLRFDAVLLICENQEVSNVIKTACICSLLMSALIASVCLLLGYEFWIYVF
                     VSLLGLSLQLIVTSIFLRNNKHLHSSLFNFIFIISIPIFQALLTHYNMVNGLLLGHSL
                     ASLLYLIIVMPILCLSVRCGSKNTNLRNILYSYNSYYTLNAGSVFINSVSNQLLPFAI
                     KFIYGVEILGLINILQRLIITPIGFILRIFIQVYNREFSFYLRGGEPQKARLVFIKTI
                     KLTFICSSITFLILLAVLFGKTLVSDIILLSGKFGTWVNVYRYVPIMLLLLAFQILVI
                     PVSQSLTFIKKHKLQFNLELIRLIGLITISIFSYLMGLTNYYFLFIYVLFQSIIYVCL
                     LHIIFKCTK"
     CDS             5857..7011
                     /note="O030_05"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ00782.1"
                     /translation="MKVFLRAPRRWVSLLASKLGCESIFENKQMFQKNRSMARNLIQK
                     IFNVWKGSSLPPLEWFIQYDRLYKVGRHIELIVEPGSKIINTKKDYLIYVENSLGLFG
                     YNTRKINSFNIFIFRKILSKSNFKGFVFYSETARSSTRELFKKINLTYLFENNDLGII
                     YPITPDVNVSEQANVKRDKFKLLFCSSSFNLKGGRELVEAINKSKFKKIIKLILITNL
                     DDSNRSFVNNNDFCEYIKFDLTEQEYIDLCLKADLIIHPTFFDTHALSLLEGIKCGKP
                     AIATNTFALKEYIEDSKTGRLIENPYEPYDKNGCANFYGVALKHALSISKLPINKEFS
                     NKLANEIDEIIENYDSYLYNCKNSESMFQRFSEREIIFQWNKLFDGLREK"
     CDS             7008..7763
                     /note="O030_06"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ00783.1"
                     /translation="MNELVSIITPTYNSQDYIIETYKSIRRQDYKLWEWIVTDDCSQD
                     ATFEILMSIVEKDSRVKLYRNSINSGAAISRNNSLAKAKGRFIAFIDSDDLWCEQKLS
                     KQIDYMISTNCAFCFTSFELIDQYGKSLEKIVDKKKIPPLNYEDMLKKNATLGCSTVI
                     LDRSKIGDFKMPLLRTGQDYALWLSILKKGFKAYHYPVVLTSYRILANSISRNKLKKA
                     CRQWQIYRTIEKIPFSSSAYYFAHYAIHAIFRK"
     gene            7912..9318
                     /gene="gnd"
     CDS             7912..9318
                     /gene="gnd"
                     /note="O030_07"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ00784.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLEMPRRILLMVKAGAGTDAAIDSLKPFLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQAAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     CDS             9433..9837
                     /note="O030_08;
                     transposase of IS3 family"
                     /pseudogene="unknown"
                     /codon_start=1
                     /transl_table=11
     CDS             9840..10052
                     /note="O030_09;
                     transposase of IS3 family"
                     /pseudogene="unknown"
                     /codon_start=1
                     /transl_table=11
     CDS             10160..11296
                     /note="O030_10"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase of ISEc1"
                     /protein_id="BAQ00785.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFDNGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAMSEKSHGREEIRLHIVCDIPDELIDFTFEWKGLKKLCVAASFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFS
                     GIRHIAINILTNDKVFKAGLRRKMRKAAMDRKYLAPVLAGSGLS"
     CDS             11274..11840
                     /note="O030_11;
                     integrase"
                     /pseudogene="unknown"
                     /codon_start=1
                     /transl_table=11
     gene            12032..13198
                     /gene="ugd"
     CDS             12032..13198
                     /gene="ugd"
                     /note="O030_12"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ00786.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            13288..14325
                     /gene="wzz"
     CDS             13288..14325
                     /gene="wzz"
                     /note="O030_13"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ00787.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLVVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGP
                     AVPKVSDIQTSLIGRYSTAFSALAETLDNQEEAEKLTIEPTVKNQPLPLAVSYVGETP
                     EGAQKQLAKYIQQVDDQVNEELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQIQQTGEDITQDTLFLLGSEALESMIKHEATRPLVFSSNYYQ
                     TRQNLLDIENLKVDDLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVL
                     GRNALRNYNAK"
     gene            complement(14422..15033)
                     /gene="hisI"
     CDS             complement(14422..15033)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ00788.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
ORIGIN      
        1 acgccatctt acgccaggga tttcataacc agattatcgr tgccaacatc accaattgta
       61 agttcagcga cttacagggg gacgctattg aatggaacgt ggcgattaac gacagtgata
      121 ttttgatctc cgaccatgtc atcgagcgca tcaactgtac caacggcaaa atcaactggg
      181 gcatcggtat aggccttgcg ggaagctctt acgataataa ttacccggaa gaccaggcag
      241 tgaaaaactt tgtcgtggcg aatatcacgg gatcggattg tcggcagttg atccatgttg
      301 aaaatggtaa acattttgtt attcgtaata tcaaagcccg caatatcacg ccggatttca
      361 gtaagaaagc aggtattgat aacgcgacag tcgctattta cggttgtgac aatttcgtga
      421 ttgataatat tgaaatgatt aatagcgccg ggatgttaat cggttatggg ataattaaag
      481 gcaaatatct ctcgataccg caaaatttcc gagtgaataa tattcagctg gataacaccc
      541 accttgctta caaattgcgc ggtatccaaa tctctgccgg gaatgctgtc tcctttgtgg
      601 cgctgactaa cattgagatg aagcgtgcgt cgcttgagct acacaataag ccgaaatacc
      661 tttttatgcg taatatcaat gtgatgcagg aatcctcagt tggaccagca ttgagtatga
      721 actttgacat gcgcaaagac gttcgcggcg tgtttatggc gaaaaaagaa acactactgt
      781 ctcttgcaaa tgttcatgcg gtgaatgaaa gagggcaaag ctccgtcgat atcgacagga
      841 taaatcacca tattgttaat gtggaaaaga ttaactttag attgccggaa cggagagagt
      901 agatttgcga ccattcctgg aaaaatggag ccatacttag gaacaatgct actgcaatcc
      961 acaacgaagc ggcgtaacat cacaagtaat tcagtaatca attcagggta attgatgctg
     1021 gcgaaaaaaa tcgaacaagc tataattcag caaccatttt acaggtggat gaaataatga
     1081 cgaatttaaa agcagttatt cctgtagcgg gtcttgggat gcatatgttg cctgccacta
     1141 aggcgattcc caaagagatg ctaccgatcg tcgacaagcc aatgattcag tacattgttg
     1201 acgagattgt ggctgcaggg atcaaagaaa tcctcctggt aactcacgcg tccaagaacg
     1261 cggtcgaaaa ccatttcgac acctcttatg aattagaatc tcttcttgag cagcgcgtga
     1321 agcgtcagct actggcggaa gtacagtcca tctgcccgcc gggtgtgacc attatgaacg
     1381 tgcgtcaggg cgaaccttta ggtctgggcc actccatttt gtgtgcgcga cctgccattg
     1441 gtgacaaccc atttgtcgtg gtactgccag acgttgtgat cgacgacgcc agcgccgacc
     1501 cgctacgtta caaccttgct gccatgattg cgcgcttcaa tgaaacggga cgcagccagg
     1561 tgctggcaaa acgtatgccg ggcgatctct ctgaatactc cgtcatccaa accaaagaac
     1621 cactggatcg tgaaggtaaa gtcagccgca tcgtcgaatt tatcgaaaaa ccggatcagc
     1681 cacagacgct ggactcagac atcatggccg taggtcgcta tgtgctttct gccgatattt
     1741 ggccggaact ggaacgcact cagccaggtg catggggacg tattcagctg actgatgcca
     1801 ttgctgaact ggcgaaaaaa cagtccgttg atgccatgct gatgacaggt gacagctacg
     1861 actgcggtaa aaaaatgggt tatatgcaag cgtttgtgaa gtatgggctg cgcaacctca
     1921 aagaaggggc gaagttccgt aaagggattg agaagctgct gatgcggagt aattgaattt
     1981 tgtaaattta acttgtgtag acactggcta cttttcatct atatcatgga acaaacaaag
     2041 gcgatttaga tggcccatca aatttgaata ataaaggaca gcatgatcgt ccaatttgat
     2101 ttataaaatt agaattgaga acatgactag ttatttttta tcaaaggatg aaagattaag
     2161 ttgatgtcga tgttaatctc tagttgtctg gtagctattg agccaggggc ggtagcgtgg
     2221 atcgagttaa ttttagccta gtgaagattt gatagtatat gaaagtatta cacattgcta
     2281 gaaatgttcc cataaaatcc ttgagaggaa atcctgtaat tttagattta attaatagat
     2341 taagtcaagc aggaatagtc aataaattag ctttcccagc tgaatatata ccatcattct
     2401 cctttttacg tggtagacca aggatattct cagagcttaa aggggaattt aatataggag
     2461 ggttagaggt tttatttgtg aacttcatta gaatccctgg gcgatggtct ttcttattta
     2521 gccgatttta ttttaacaaa aaaaagcttg ataagttttc agagggagta aattgtattc
     2581 atgctcatta tataatcccg gatgggttgg tagcttatga attagcgaaa aataaaaata
     2641 ttccttatat tgtaacaatt agacaaggag atttagatag aattaataaa ctgaaacatg
     2701 gagattgctc ttataagcaa tatgaaaaag ttgtatctaa tgctgcattt ttgttggcaa
     2761 taaatccaaa tatcgcaaat gaaataaaaa ggatttttca gaaaaattgc attacaattc
     2821 cacatggagt tgataaaagc ctcattgaat acgggattga gaataggata cataaaaaga
     2881 taatagtggc agcacaattc attaaaagaa aaaatattga ttgggttatt aaagttttta
     2941 actcaatgac ggatcaaggg atcagcaatt acactttaac tattattgga gagggggaat
     3001 tagaaaatga tttaaaaaaa ctagcgagtg gaaatgataa gataattttt aaaccatggc
     3061 tgtctaaaaa agaactgatg ggggaattcc tattatctga tatttttatc atgcctagtg
     3121 atcaagaaac ttttgggatg gtttatattg aagctgcagc taaaaattgc ttgataatag
     3181 gtaaaaaaga tacgggggtt gatggttatt ttgagaatga aaaaaatgca ttgtttattt
     3241 ctacggttgg agaactcgag atagttatta aaaaaattct attaggtttg attgatatta
     3301 gagaaatgca aaatctcggt acaatcttag tagaaaaaaa tatgacttgg gacaaggtta
     3361 caagtaatta tataaaactc taccataaag ccatagcaaa tgttcagaat cagtgatttt
     3421 ttcaaagtta atctattatt tctcatactt acattctctt atgtcaactt gatctctaaa
     3481 caattttctt acatgggttt taaagatgac tttaatatag agaggtttgg cgtaagtttg
     3541 attttctgtg tattagttgc aatttattac gctagtgtac aagagttctt caataaattt
     3601 ataagtacat tatttctgat ttttgtaatt atgcctaatc ttgtcctttt tagtggaatg
     3661 ggaggggata tcagaataat tatatggtgc gtactatcat taccgatcac tttgttttta
     3721 ttgcgtatct tgcctgtgat taagttacca gtaattaaag ataaacaggt cggagccctc
     3781 ctttgggggt tactaatttt atgtattata cctgtattat tagcacatgg aattaatttc
     3841 aatcttaagg tttttttgct agatgtgtat gatattagac gtgagtcacg tttggctaat
     3901 accatggctt cagtatacgc atatttctgg ttagctaaag taatatgtcc aatagcatta
     3961 gtgtatgcca ttgaaagaaa aaaaatagta atggcaatga ttttcacgct agtgctttta
     4021 tatcttttta tgacaactgg tcataaatca gtatttttta ctgttattct tatcatggtt
     4081 atgtacaaag gaggaatgga ttatcagaaa aaaagtaatt acatatttaa tggtgcgttg
     4141 atattattta ttttctctat tcttcttaca gccatctttt caataaatat actggagtca
     4201 ctttttatta gaaggttgct ttttattcct gctttattaa atacatacta ttttgatttt
     4261 tttgataaca aatttgtcta ctattctagt tcttatttat catcgataat cgattatcca
     4321 tatgatagac caataccaga agtaataggg ctcaattatt ttaatagcga tgaaatgagt
     4381 gcgaataatg gttatattag tgatggcttt gcgaacgctg gaagtattgg gatatttatt
     4441 aatatagtcc tggcttctat cttgttaaag atttttaagg attatgatgt taacccaaag
     4501 tacgcagggc tcatatttgt cagtttttat gctattcagg gtagcgcaat gtcgacagta
     4561 ttaatgactc atggtgggat tttattgtta atactggtgc caatggtttt gggtagacga
     4621 aaatgattaa aaaaatacta aaactattat ctggtcccgc tttgggccac ctaataacga
     4681 tagctttaac accctatttt atgtaccatt actcgccttc tatctttggt atctatgctt
     4741 tttttacagc aatacttgga gtatttgttt caataagcac tctacgtttc gatgcagttt
     4801 tacttatttg tgagaatcag gaagtgtcta atgttattaa aactgcgtgc atatgttctt
     4861 tattgatgag tgctttaatt gcttcagttt gtttattact tggctatgaa ttctggatat
     4921 acgtctttgt ttctttactt ggtctttcat tacaactaat tgttactagt attttcctaa
     4981 ggaataataa acatttacac tcttcattat ttaattttat atttattatt tcgattccta
     5041 tttttcaggc gcttttaacg cattataata tggtgaatgg tctgctttta ggacactcct
     5101 tggcttcatt actatattta attattgtga tgccaatatt atgcttaagt gttagatgtg
     5161 gttctaaaaa tactaatctg cgtaatattt tatattctta taatagttat tacacgttaa
     5221 atgccggctc tgtttttatt aattcggtat ctaaccagtt attacccttt gctataaaat
     5281 ttatttatgg agtcgaaatt ctcgggttga taaatatttt acaaagatta ataattactc
     5341 caattggatt tattttacgt atatttattc aagtttacaa tagagagttt tctttttatt
     5401 tgcgtggggg ggagcctcaa aaagcacgtt tggtatttat aaaaaccata aaacttactt
     5461 ttatatgttc atcgattact tttttaattc tattagcggt gttattcggt aaaacactcg
     5521 tgagtgatat tattttgctc tcgggaaaat ttggaacttg ggttaatgta tatagatatg
     5581 ttcctattat gttactttta ttagctttcc aaattttagt tattcctgta tcccaaagct
     5641 taaccttcat aaaaaaacat aagttacaat ttaatcttga gttgataaga ttaattggat
     5701 taataacgat atcaatattt tcctatttaa tgggtttgac caattactat tttttattta
     5761 tatatgttct tttccaatcg ataatatatg tttgtttact gcatatcatc tttaagtgta
     5821 caaaataaaa atgatcgtta aaactaaggg taggatatga aagtgttctt aagagcgcca
     5881 agacgatggg tttctttatt ggcatcaaaa ttgggctgtg agtcaatttt tgaaaacaaa
     5941 caaatgtttc aaaaaaatcg aagtatggcg agaaatctta tccagaaaat atttaatgtt
     6001 tggaagggaa gctctttgcc tccacttgaa tggtttatac aatatgatag attatataaa
     6061 gttgggcgtc atattgaatt aattgtagag ccaggttcta aaataattaa caccaaaaaa
     6121 gattatttaa tttacgttga gaactccttg gggttgtttg gatataatac aaggaaaata
     6181 aactctttta atatatttat ttttagaaaa atactttcaa aaagtaactt taaaggtttc
     6241 gttttttatt ctgaaacggc tcgttcttca actcgcgaac tttttaaaaa aataaatctc
     6301 acatacttat ttgaaaataa cgatttgggt attatctatc ctataactcc tgatgtcaat
     6361 gttagtgaac aagctaatgt taaacgagat aaatttaaat tgttattctg ttcaagtagt
     6421 ttcaatctaa aaggtgggag ggaattagtt gaagccatta ataaatctaa gtttaagaaa
     6481 ataataaaat taatcttaat taccaatcta gatgattcaa ataggagttt tgtgaataat
     6541 aatgattttt gtgagtatat aaaatttgat ttaactgagc aagaatatat tgatctttgc
     6601 cttaaagctg atttgattat acatcccact ttttttgaca cgcatgcttt atctctgtta
     6661 gaaggaataa aatgtggaaa gccagctatt gctacgaata catttgcttt aaaagaatat
     6721 atcgaagatt caaaaacagg tagactcatt gaaaatcctt atgagccata tgataaaaac
     6781 gggtgtgcca atttttatgg ggttgctttg aaacatgcac tctcaattag taaactccca
     6841 atcaacaaag aattctctaa taaattagca aatgaaatag atgaaataat agaaaactat
     6901 gactcttatt tatataattg taagaattca gagagcatgt ttcaacgatt ttctgaaaga
     6961 gaaattattt ttcagtggaa taagttgttt gatggcttga gggaaaaatg aacgaattag
     7021 tttctattat cactccaacc tataactcac aggattatat tattgaaaca tacaaatcta
     7081 tacgacgcca agattataaa ttatgggagt ggattgtaac cgatgattgt tcccaagatg
     7141 caacatttga aattcttatg agcatagttg aaaaagattc tcgagtaaaa ttatatagaa
     7201 atagcatcaa ttctggcgca gctatctcac ggaataattc acttgcaaaa gcaaaaggtc
     7261 ggtttattgc atttattgat agtgatgact tatggtgtga gcaaaaatta tccaagcaaa
     7321 tagattatat gattagcaca aattgtgctt tttgctttac ttcatttgaa cttatagatc
     7381 aatatggcaa atctttggaa aaaattgtgg ataaaaagaa aataccaccg ttgaattatg
     7441 aagatatgct aaagaagaat gctactctag ggtgctcgac agttatttta gatagaagta
     7501 agattgggga tttcaagatg cctttactta gaacagggca ggattatgca ttgtggcttt
     7561 caatattaaa aaaaggattt aaggcttatc attatccagt tgttttaact tcctatagaa
     7621 ttttagccaa ctcgatctcc agaaataaac ttaaaaaggc ttgtcggcaa tggcaaatct
     7681 ataggactat cgaaaaaatc ccattttctt cttcagctta ttattttgct cattatgcca
     7741 tacatgctat attcaggaaa tgaacccata atcttatgat ttaatttcat tttaatgctt
     7801 agcaaaattc actaatttat agccaataat tgatttcttg taaagaaaat attaagttta
     7861 acagtttata gttaaattca tttttaaggt tttagctgac aggagttaac aatgtcaaag
     7921 caacagatcg gcgtcgtcgg tatggccgtg atgggacgca accttgcgct caacatcgaa
     7981 agccgtggtt ataccgtctc tattttcaac cgttcccgtg aaaagacgga agaagtgatt
     8041 gccgaaaatc caggcaagaa gctggttcct tactatacgg tgaaagagtt tgttgaatct
     8101 ctggaaatgc ctcgtcgcat cctgttaatg gtgaaagcag gtgcaggaac ggatgctgct
     8161 attgattctc tcaagccatt cctcgataaa ggtgacatca tcattgatgg cggtaacacc
     8221 ttcttccagg acaccattcg tcgtaaccgt gagctttctg cagaaggctt taactttatc
     8281 ggtactggtg tttccggtgg tgaagagggc gcgctgaaag gtccttccat tatgcctggt
     8341 ggccagaaag aagcctatga actggttgcg ccgatcctga ccaaaatcgc cgcagtagct
     8401 gaagacggtg agccatgcgt tacctatatt ggtgccgatg gcgcaggtca ctatgtgaag
     8461 atggttcaca acggtattga atacggtgat atgcagctga ttgctgaagc ctattctctg
     8521 cttaaaggtg gcctgaacct caccaacgaa gaactggcgc agacctttac cgagtggaat
     8581 aacggtgaac tgagcagcta cctgatcgac atcaccaaag atatcttcac caaaaaagat
     8641 gaagacggta actacctggt tgatgtgatt ctggatgaag cagcaaacaa aggtaccggt
     8701 aaatggacca gccagagcgc gctggatctc ggcgaaccac tgtcgctgat taccgagtct
     8761 gtgtttgcac gttatatctc ttctttgaaa gatcagcgtg ttgccgcatc taaagttctc
     8821 tctggcccgc aagcacaggc agctggcgac aaggctgagt tcatcgaaaa agttcgtcgt
     8881 gcgctgtatc ttggcaaaat agtttcttac gctcagggct tctctcagtt acgcgccgcg
     8941 tctgaagagt acaactggga tctgaactac ggtgaaatcg cgaagatttt ccgtgctggc
     9001 tgcatcatcc gtgcgcagtt cctgcagaaa ataaccgatg cttatgccga aaatccgcag
     9061 atcgctaacc tgctgctggc tccgtacttt aagcaaatcg ccgatgacta tcagcaggcg
     9121 ctgcgtgatg tcgttgctta tgcagtacag aacggtatcc cggttccgac cttcgccgct
     9181 gcggttgcct attatgacag ctaccgcgcc gctgttttgc ctgcgaacct gatccaggca
     9241 cagcgtgact atttcggtgc acatacttat aagcgcattg ataaagaagg tgtgttccat
     9301 accgaatggc tggattaatc tgatttaaat caattaacca acacaaggcc cggagaaacc
     9361 ctccgggctt ttttattata catgacccgt cctgaatagc gttgacacgt tcctgactta
     9421 aatccggaga acgtgatgat gactgagttc aaacgcaccc aacgtgatta ccctctatcc
     9481 tttaaaatag ctgttgttga gcaggttgaa aaaggcgaga tgacctataa acaggcccag
     9541 cagcggtatg gcattcaggg gcgctccacc gttcttgtct ggttacgtaa atatggtcgg
     9601 cttgactgga gtcctggact tcctgacctg gtgaagagga aactgcctgt gactcagaca
     9661 actatcccgc tcacacccga gcaaagaatc agggaacttg aagcgcagct tgagctggcg
     9721 aaccagaaag ctgagttttt tgagtcagtt atcaatgtcc tgaaaaatga ttacggggtg
     9781 agtatcgtaa aaaagcggcc cggcaagtcc tcgcgcaaag tccggccccc aaaataactg
     9841 tgacgcgtgc atgtctgttc ctgggacaca gcagacaggc atggtatcag gacaacgcaa
     9901 ggcgcagaaa acggcaggaa catcatgctc aggttcttga ttttgttgcc cgtgtcagat
     9961 gtcgtcagcc ccgaatcggt gcgcgtaagc tgcactatct gctgaacact caggccaggg
    10021 aaagatcacg taacgctgct tttttgtact aaataattcg cattttatgt ttaaaaattg
    10081 agatattcct cattacctga agctgttttt tattgcttat acatgatcaa atactcctta
    10141 cataattaag gagaacaaaa tggaacttaa aaaattgatg gaacatattt ctattatccc
    10201 cgattacaga caagcctgga aagtggaaca taaattatcg gatattctac tgttgactat
    10261 ttgtgccgtt atttcgggtg cagaaggctg ggaagatata gaggattttg gggaaacaca
    10321 tctcgatttt ttgaagcaat atggtgattt tgataatggt attcctgttc acgataccat
    10381 tgccagagtt gtatcctgta tcagtcctgc aaaatttcac gagtgcttta ttaactggat
    10441 gcgtgactgc cattcttcag atgataaaga cgtcatcgca attgatggaa aaacactccg
    10501 gcattcttat gacaagagtc gccgcaaggg agcgattcat gtcattagtg cgttctcaac
    10561 aatgcacagt ctggtcatcg gacagatcaa gacggatgag aaatctaatg agattacagc
    10621 tatcccagaa cttcttaaca tgctggatat taaaggaaaa atcatcacaa ctgatgcgat
    10681 gggttgccag aaagatattg cagagaagat acaaaaacag ggaggtgatt atttattcgc
    10741 ggtaaaagga aaccaggggc ggctaaataa agcctttgag gaaaaatttc cgctgaaaga
    10801 attaaataat ccagagcatg acagttacgc aatgagtgaa aagagtcacg gcagagaaga
    10861 aatccgtctt catattgttt gcgatatccc tgatgaactt attgatttca cgtttgaatg
    10921 gaaagggctg aagaaattat gcgtggcagc ctcctttcgg tcaataatag cagaacaaaa
    10981 gaaagagcca gaaatgacgg tcagatatta tatcagttct gctgatttaa ccgcagagaa
    11041 gttcgccaca gcaatccgaa accactggca cgtggagaat aagctgcact ggcgtctgga
    11101 cgtggtaatg aatgaagacg actgcaaaat aagaagagga aatgcagcag aattattttc
    11161 agggatacgg cacatcgcaa ttaatatttt gacgaatgat aaggtattca aggcagggtt
    11221 aagacgtaag atgcgaaaag cagccatgga cagaaagtat ctggcgccag tccttgcggg
    11281 gagcgggctt tcgtaatctt tccctgggcc ctgaacaggt taccgctctt gaaccagaac
    11341 aggtctgggt cgccgatatt acttaccttc cactgcgcaa tggtacggcc tatctgagtc
    11401 tggtcaccga tgcctgctcc agaaaaatta tgggctacca tgttggggaa aacctgcaga
    11461 cagaaaatgt ggtaaaagcg ttcagacagg cgctgaggct gagaaaaacg acgagtcccc
    11521 tggtgcatca ttctgacagg gggctgcaat actgttcagc gctctatcag tcagttcatg
    11581 aaagaaatgg aataacctgt tcaatgactg atggttatga ctgctaccag aacgcactgg
    11641 ctgagcgaat aaacggtatc ctgaaaaatg agtttttact ctcgcgtcct gcagacctgg
    11701 cgcaggcccg ggaaatggtc aaagagtccg tggcaattta taactatgag cggccacacc
    11761 aggctctgaa atacaaaacg cccgatgacg ttcatcaggc gttttacaga cagaaaagtg
    11821 tcaacttata tcaggactag tcaacaaagc ggcaggttat ggcttttttt tataatttat
    11881 ggttaaaaaa gtgatataat acagcggctg catagcagga tcgctgcctt gacagttcat
    11941 ctgcatcagc gttaaaaatt ccgcagtaga tgaagttgtg gtggtgggtt aatgaccact
    12001 ctaaatgttt aaccggaaga agtcagagct aatgaaaata acaatttcag gaacaggtta
    12061 tgttggtctt tcaaatggta ttctgattgc gcaaaaccac gaagtggttg cactggatat
    12121 cgttcaggcc aaagtggaca tgcttaacaa gaagcagtca ccgattgttg ataaggagat
    12181 tgaagagtat ctggcgacta aagatctcaa tttccgcgct acgacagata agtatgacgc
    12241 gtataaaaat gccgattacg ttattattgc cacgcctaca gattatgatc cgaaaacgaa
    12301 ttattttaat acctcaagcg tggaagcggt catccgcgat gtgacagaaa ttaatcccaa
    12361 cgcggtaatg gtgattaaat ctacaattcc ggtaggtttc accaaatcaa ttaaagaacg
    12421 tctgggtatc gataacctta ttttctcccc cgaattcctc cgtgaaggta aagctcttta
    12481 cgataacctt catccgtcac gtattgtcat cggtgagcgt tcagaacgcg cagaacgttt
    12541 tgcggcgtta ttacaggaag gcgcgattaa gcaaaatatc ccgactctgt tcaccgattc
    12601 aaccgaagca gaagcgatta aactgtttgc taatacctat ctggcgatgc gcgtagcata
    12661 ctttaacgag ctggatagct atgcagaaag tttaggtctg aatactcgcc agattatcga
    12721 aggcgtttgt ctcgatccgc gtattggcaa ccattacaac aatccgtcgt ttggttatgg
    12781 tggctattgt ttgccgaaag ataccaagca gttactggcg aactatcagt ctgtgccgaa
    12841 taacctgatc tcggcaattg tcgatgcaaa ccgcacgcgt aaagatttta ttgccgatgc
    12901 cattttgtca cgcaaaccgc aagtggtggg tatttatcgt ctgattatga agagcgggtc
    12961 agataacttc cgtgcgtctt ccattcaggg gattatgaaa cgtatcaagg cgaaaggcgt
    13021 tgaagtgatc atctacgaac cggtgatgaa agaagattca ttcttcaact ctcgcctgga
    13081 acgtgatctc gccaccttca aacaacaagc cgacgtcatt atctccaacc gtatggcaga
    13141 agagcttaag gatgtggcag acaaagtcta cacacgcgat ctttttggca gcgactaaca
    13201 tcctgttatc agggcgattt tcgccctgat tctcttatgt tccctttgta ataattcatt
    13261 atttttatca tttatcctat agcattcatg gcgattatcg ctaaactatg gcgacttaaa
    13321 attatttcgt ccgttagggt aatgatgaga gtagaaaata ataatgtttc tgggcaaaac
    13381 catgacccgg aacagattga tttgattgat ttactggtgc agttgtggcg tggcaagatg
    13441 acaattatca tctctgtcat tgttgctatt gccctggcta tagggtatct ggtcgttgcc
    13501 aaagaaaagt ggacttcaac agctatcgtt actcaacctg atgtggggca aatcgctggt
    13561 tataccaacg cgatgaatgt gatttacggt ccggctgtac cgaaagtctc cgacattcag
    13621 acttcactta tcggacgtta cagcactgca ttctcagcat tagcggaaac gctggataac
    13681 caggaagaag cagagaagct gaccattgag cctaccgtta aaaaccagcc gttaccactg
    13741 gcggtgtctt atgttggtga aacacctgaa ggtgcacaga aacagctggc gaagtatatc
    13801 cagcaagttg acgaccaggt aaatgaagag ctggaaaaag acctgaaaga caacatcgcg
    13861 ctgcgtatga aaaacttgca ggattctttg aagactcagg aagtagttgc gcaggagcag
    13921 aaagatctgc gtatccgtca gattcaggaa gcgttgcagt atgcgaatca ggcgcaggtg
    13981 acaaaaccgc agattcaaca gactggcgaa gatatcacac aagatacgtt gttccttttg
    14041 gggagcgaag cgctggagtc gatgattaag catgaggcga cccgtccgtt ggtgttctca
    14101 tcaaactact atcagactcg tcaaaacctg ctggatatcg aaaacttaaa ggttgatgat
    14161 cttgatattc atgcttaccg ttatgtgatg aaaccgacgt tacctattcg tcgcgatagc
    14221 ccgaaaaagg caattacctt gattctggcg gtgctgctgg gcggcatggt tggcgcgggg
    14281 attgtgttgg ggcgtaatgc tctgcgtaat tacaacgcga agtaatcttt gcagctttaa
    14341 agaaaacggg cagggtggtg acaccttgcc cgttttcttt gccggatgcg acaacaacat
    14401 cgcatccgct taccccgcaa ctcactgatg ccgtttacgc aggttctcaa ttaccgtcgt
    14461 taaatccaga tcctgatcct gcaacagcac cagcaggtga tacatcaaat ccgatgcctc
    14521 gttggtcagc tcaaagcggt catgtaccgt tgctgccagt gcggtttcca cgccttcttc
    14581 gcccactttc tgcgcaatgc gtttggtgcc gctggcatac agtttggctg tgtaagaggt
    14641 ttccgggtcg gcagatttac gttcggcgag cagttgttcc agttgataca ggaacagcca
    14701 ctggtgagcg gtgttgccga agcagctgct ggtgcctttg tggcaggtcg ggccgatggg
    14761 attcgccagc actaataaag tgtcgttgtc gcagtccggc gcaatactca ctacgttgag
    14821 gaaattgccc gacgtttcgc ctttggtcca cagtcgctgt ttagtgcgcg agaagaaggt
    14881 gactttgccg ctttcgatgg ttttgtctaa ggcttccggg ttcatatagc ccagcattaa
    14941 cacttcgcca gataccgcgt gttgtacaat caccggcatc agtccgtcgg ttttttccca
    15001 gtccagttcg cgacgttgtt gttctgttaa catatcctga tctccacgcc ctgtgttgcc
    15061 aggtacgctt ttaattcacc aatattgatt atttgtttgt ggaatacgga agctgccagc
    15121 gcaccgtcaa cgtcggcatc gcggaaagct tcgaggaagt gttccatggt gcccgcgcca
    15181 ccggaggcaa ttagcgggac gtggcaaact tcacgtactt ttttcagttg ttcgaggtcg
    15241 taaccgttac gcacgccgtc ctgattcatc atgttgagga cgatttctcc ggcaccgcgt
    15301 ttttgcactt cctgtaccca gtcgagcgtt tcccactgag tgacgcgggt gcggc
//