<pre>LOCUS       DQ462205                8579 bp    DNA     linear   BCT 06-JUL-2010
DEFINITION  Escherichia coli O28ac O antigen gene cluster, complete sequence.
ACCESSION   DQ462205
VERSION     DQ462205.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8579)
  AUTHORS   Fratamico,P.M., Yan,X., Liu,Y., DebRoy,C., Byrne,B., Monaghan,A.,
            Fanning,S. and Bolton,D.
  TITLE     Escherichia coli serogroup O2 and O28ac O-antigen gene cluster
            sequences and detection of pathogenic E. coli O2 and O28ac by PCR
  JOURNAL   Can. J. Microbiol. 56 (4), 308-316 (2010)
   PUBMED   20453897
REFERENCE   2  (bases 1 to 8579)
  AUTHORS   Liu,Y., Debroy,C. and Fratamico,P.M.
  TITLE     Molecular characterization of the Escherichia coli serogroups
            O28ac, O117 and O126 O antigen gene clusters and identification of
            their specific genes
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8579)
  AUTHORS   Liu,Y., Debroy,C. and Fratamico,P.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2006) ERRC-USDA, 600 E. Mermaid Lane, Wyndmoor,
            PA 19038, USA
FEATURES             Location/Qualifiers
     source          1..8579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O28ac"
     misc_feature    615..8362
                     /note="O antigen gene cluster"
     gene            615..1685
                     /gene="wzx"
     CDS             615..1685
                     /gene="wzx"
                     /note="O antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ABF01008.1"
                     /translation="MSLILTDFGFNLSSTEYIARNCQSKDKINKHIGAVFQIKILLIF
                     LCVIFLYFLVNDQLQQFKIEFFFYSCIAIIGQTFQSLWFFQGVEKMKRITLFLTISKF
                     SYVILTFVIVKKPEQGRFVILAYALSQVIGALLSQYYMRKEGYKYTPANLSSMLGVLR
                     TSALFFWSRLAVSTYTTASTLVVGAAGHVQAGYYAASEQLYKAGQNITTPVSQALYPY
                     MTRAKNWALFFKMMSILTVVMITASLTAIWLSQWLIALIFGPEYIVSQNVFIVFMLAL
                     IVTFVSTNFGYPVFSALGKVKVANYTVIFGALTHVVILGVLFCLGEISAYNVVRAVLF
                     TEIVILILRMFFFVKLYRREVK"
     gene            1682..2860
                     /gene="wbeS"
     CDS             1682..2860
                     /gene="wbeS"
                     /note="glycerol phosphotransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbeS"
                     /protein_id="ABF01009.1"
                     /translation="MIIKKVISKLFGLFFCALDYVWKKQDINIFGAGRGSRYFDNSKT
                     LFEYYISQGDRAYWFYTGKKPDVVGISPDAFVQANSLYGMLIALRAKKAFACYGSLDF
                     WLYRFSRKTVSVQLWHGIPIKKVFFGEKNLTQRKKLQYLFEISAFQYFIVSSKLEQYI
                     ISAQTGMHLDRVKVLGYPRNDNLFKSANEVIIPEVVALNSNFKKVVLFAPTFRNNDYS
                     VFSSFNDDEWNVFFDFLKTENFLCIFRAHAVEFLQNNDDNAKRLSKYSSNVIFLGQDK
                     YQDAQDLMVYSDILISDYSGMIPDYLLLDKPIIRFIFDEQQYDNERGVNFLQKNLPIG
                     SAAYNMPTLIEALRQCVESTEESVAVNAFAKKMYHDNLCGKYSEKIYNYFNSLSQGKV
                     "
     gene            2857..4236
                     /gene="wzy"
     CDS             2857..4236
                     /gene="wzy"
                     /note="O antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ABF01010.1"
                     /translation="MKQARIKPFSYLSLSFFVLFLIFLNISAVSFDEYLTDLQYSYAY
                     VLFAISLVAVYYAQGESYTSLGFLFNTCLFLFIGGRFIATTFGIDSESVFTLDFMTTY
                     IPTSDEKLEIYTRVVSFQIFLNLGYLFLRKEDSLLNLNKNDNSLPGRLLFLIVIVLFL
                     LCLVEQYSMLSKAMSEGYLARYGDQAESYDAGAAFKYTLLFVFTGLLFATCKKKYIII
                     NFMIYMTLATIIILIGARGAFVSTILMGVWLYGLNKKISLKKLSIIFLSLAGILLIIM
                     NFSARGNYYSNELIDSLLRFLYDQGNTLAVVGYVRTITDYPVFAYISTFLPGSSFFAS
                     MFHGGSDLNSYMLSFPAYANYVANPNMYFAGYGLGWSIISSVEMFSADILFIFCIFSV
                     LIGMFLKFANEQAKRNSFWFGAVACLCAKILFTARADIKTIIPLFMYYAIMYLIISTA
                     MRILRQRYG"
     gene            4229..5239
                     /gene="wbeX"
     CDS             4229..5239
                     /gene="wbeX"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbeX"
                     /protein_id="ABF01011.1"
                     /translation="MDNRDVMFSVIVPCYNVVSHINETVASLNKAIDSACTTVEVIFV
                     NDGSTDKTLDVLNEICSNKPSYRVIDQENGGVSNARNTGIRESTGKYILFLDSDDTYK
                     NNIFSILSNISFSDDIVFFSYEKIDQSGNTRVYTIPNSYMAENSITVLKDLFSKKIYL
                     NICALAVNRDCIFQNNIFFDESIQHCEDLQFIIKMITYSKSFQFINEPLFTYNYTPGS
                     AVNSKVDEKHYSKFIAFENMRDVFIKNDKYKELIPAYDFYVATVYLLLLKTIVSNGVV
                     NNKQLSKFVEYSFILKRKMELPPNTMGAFVLIFKIINYIFPMKVKFLMMSFILRMNKG
                     LR"
     gene            5236..6153
                     /gene="wbeY"
     CDS             5236..6153
                     /gene="wbeY"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbeY"
                     /protein_id="ABF01012.1"
                     /translation="MSITNKTIALVIVTYNRCNLLMEMLSSIENMSVKPDIVYVIDNN
                     SSDNTSSVVTECDSRKNINIKYHNTGYNAGGAGGFYIGSKMAYEDGWDRIWLADDDIV
                     LDKECLSNAMEYDDGRTILQPMRYNMDGSCAEISAIQYDLSNPFYLRPKRKTVQNIFN
                     KNILSYDIQSIPFEGPIIPREVFNVIGFPDERFFIFNDDLDFAIRAQRAGFSIKCITN
                     AKIVRKIPFVQSVALKTWKGYFMFRNYFRVQKVYGLSPLIYLRILLVFCLALGHSLVR
                     MDINSIKMLCGALKDGLSQEFKLTEKYKP"
     gene            6150..7220
                     /gene="wbeZ"
     CDS             6150..7220
                     /gene="wbeZ"
                     /codon_start=1
                     /transl_table=11
                     /product="WbeZ"
                     /protein_id="ABF01013.1"
                     /translation="MSTTDSPNIKTSNNNFDIVRLILALVVVFSHIRDLNNINSRFFS
                     IFSGDFAVECFFVISGYLIIRSFKNNQNIRKYIFSRGMRILPMYYVSIVSFFVILFFI
                     SHLSVVEYLKEGGIRYLVFNGVFLNFIQPSLPGVFVDNPRISAVNGALWTIKVEILFY
                     IFVPFFVLFIKKINRHIIPIFIILILSSIVFKWIVTDYHQILHIPEQLVNQLPSVLSY
                     FLLGGLVNYLDLSKISKKTVFWFFIVSAFYLLLSSKTSLDLLVRPFVIAGFILPICLQ
                     RKFLISVPEKMGDLSYGIYVTHFPLVQLLIAVGLYNSFWLGFCLTFTSLFILSFISWH
                     LIEKPALRFAKSAGKNKVVRGV"
     gene            7217..8362
                     /gene="glf"
     CDS             7217..8362
                     /gene="glf"
                     /note="UDP-galactopyranose"
                     /codon_start=1
                     /transl_table=11
                     /product="Glf"
                     /protein_id="ABF01014.1"
                     /translation="MRIAIVGAGFSGAVIARELAEHGHSVDIFEKRDHVAGNCYSKRD
                     PDTNVMVHVYGPHIFHTDDKEVWEYINKFGMFEPYVNRVKAITKGAVYSLPINLHTIN
                     QFFHKTLNPAEAKDFINSLCELFEFEPITFEQQALSFIGRDLYEAFFRTYTIKQWGLE
                     PSELPASILKRLPVRFNYNDNYFSHRYQGMPRDGYTNIISAILDHASIAVKLNTDFKK
                     NVGNEYDHVFYSGTIDGYFDYSHGRLPYRTLDFVTERHKGDYQGTAVINYCDDSEKFT
                     RITEHKYFSPWEEHSNTIIYKEYSRTCTETDSPYYPIRQVQEKELLRKYVDLANQTNN
                     VSFIGRLGTYRYLDMDVTIREALNAVKLFMKIKKENEKVPAFFTEIL"
ORIGIN      
        1 attggtagct gtaagccaag ggcggtagcg tgaatattat gagagagcta tgaagaaaat
       61 tataaccttt gggacttttg acgttttcca tgttgggcat gtgaatattc ttgaaagggc
      121 tgccagtctt ggcgactatc taatagttgg cgtctcttct gatgagttga attattcaaa
      181 aaaacaacgc tatcctattt attctcaaga agatcgaacc aaaattattt catcactaaa
      241 gtttgtcaat gaagttttca tagaagaatc ccttgagaaa aagctcgagt acattaaaac
      301 atataacgcg gatattcttg ttatggggga tgattgggct gggagattcg attgggtgaa
      361 aagtgattgt gaagttattt atctgcctcg aacaccatct atatcgacta ctgaaattat
      421 agaagtcgtc aagtctatta ggtagggcag gtagattgaa acttaagcag aatgtattgt
      481 ttggtaatat tgcggcgctg gcgatattac aattagctac atatatcacg cctttgttaa
      541 ttgtccctta tcttactcga gtgttaggtc cgggaggcta tggcactgtt gcaatttgtt
      601 tgtcaataat tcagatgtca ctaatattga ctgactttgg ttttaatctt agttctacag
      661 aatatatagc aagaaattgt caatctaaag ataaaataaa taaacatatt ggtgcagttt
      721 ttcaaattaa aatattattg atttttctgt gtgtgatttt tctttatttt ttagtaaatg
      781 accaattgca acaatttaaa atagagtttt tcttttattc atgcattgct attattgggc
      841 aaacatttca atccctatgg tttttccaag gggtagaaaa gatgaagcgg attacacttt
      901 ttttaactat atcaaagttt tcatatgtta tcctgacttt tgtaattgta aaaaaaccag
      961 agcaaggacg atttgtcatt cttgcttatg cattatctca agtcattggc gcattattat
     1021 ctcaatatta catgcgtaaa gaaggttata aatatacccc tgcaaattta tccagcatgt
     1081 tgggggtttt acgtaccagt gcattattct tttggtcccg acttgctgtc tccacatata
     1141 caactgcgag cacattggtc gtaggagctg caggacatgt gcaagctggt tattatgctg
     1201 ccagcgaaca actgtataag gcaggtcaaa atataacgac cccagtcagc caggcattat
     1261 atccttatat gacacgggca aaaaattggg ccctgttttt taaaatgatg tctattctta
     1321 cggttgtgat gattaccgct agcctaactg ctatttggtt aagtcagtgg ctgattgctt
     1381 taatctttgg tccagaatat atagttagcc agaatgtatt catagttttc atgcttgccc
     1441 taatagtcac atttgtaagt acaaattttg ggtaccctgt attttccgcg cttgggaaag
     1501 ttaaagttgc aaattacact gttattttcg gtgctttaac gcatgtagtt attctgggtg
     1561 ttttgttttg tttgggtgaa ataagtgcct ataacgtagt tagggctgta ttatttacag
     1621 aaatagtcat attgatattg cgaatgttct tctttgtgaa gttatacagg cgagaagtaa
     1681 aatgataatt aaaaaagtta tatccaaatt atttggcttg tttttttgtg cgctggatta
     1741 cgtttggaaa aaacaagata taaatatatt tggagcaggt agaggctctc gttattttga
     1801 taactcgaaa acactctttg agtattatat atctcaaggt gatcgagcct actggttcta
     1861 cacaggaaag aagccagatg ttgttggaat ctctcctgat gcatttgtac aggctaacag
     1921 tttgtatgga atgttgattg cattacgtgc aaaaaaagca ttcgcatgtt acggttcttt
     1981 agatttttgg ctatataggt tttctcgtaa aacagtatcc gttcaattat ggcatggcat
     2041 acccatcaaa aaggttttct ttggtgagaa aaatttaacg cagcgtaaaa aacttcagta
     2101 tttatttgag atttcagctt ttcagtattt catagtatcc tcaaaacttg aacagtatat
     2161 tatttcagct caaaccggga tgcatctgga tcgagttaag gtcttaggtt atcccaggaa
     2221 tgataattta tttaaatcag caaatgaagt gattatccct gaagtagttg cattgaacag
     2281 taatttcaaa aaagttgttt tattcgcacc aacatttagg aataacgatt attctgtatt
     2341 tagttcattt aatgatgatg aatggaatgt ttttttcgat ttcttaaaaa cagagaattt
     2401 tctttgtatc tttcgagcgc atgctgttga gtttctccaa aataatgatg ataacgctaa
     2461 aagattgtct aaatactcaa gtaatgtcat ttttcttggg caagacaaat atcaggatgc
     2521 acaggattta atggtttaca gcgatattct gattagtgac tattccggta tgattccaga
     2581 ttacttgcta ctggataaac ctatcatcag atttattttt gatgagcagc agtatgataa
     2641 cgagcgtggc gttaactttt tgcaaaaaaa tctgcctata ggctcggccg cgtacaatat
     2701 gccgacatta atagaagcat tgagacaatg tgtagaaagc acagaagagt cggtggcagt
     2761 aaatgcgttt gcaaaaaaaa tgtatcacga taatttatgt gggaaatatt cagaaaaaat
     2821 atataactat tttaattctt tatcacaggg gaaggtatga aacaagcaag aataaagcct
     2881 ttctcatatt tatcattatc tttttttgtg ttgttcttaa tttttttaaa tatttcagca
     2941 gtttcattcg atgagtatct gacagacctt caatattcat acgcctatgt gctgtttgct
     3001 atatctcttg tggcggtcta ctacgctcaa ggtgagtctt atacgagtct tggtttcttg
     3061 tttaatacct gtttattttt gttcataggc ggtcgtttta ttgcgacaac gtttggaata
     3121 gacagtgagt cggtgtttac tttagatttc atgactacat atataccaac atcggatgag
     3181 aaattagaaa tatacacaag agtagtatca tttcaaatat ttttaaattt gggctatttg
     3241 tttttacgta aggaagatag tttattaaat ctgaataaaa atgacaattc acttccgggt
     3301 cgtcttttat ttttaattgt tatagtgctg tttttattat gccttgtgga acaatactca
     3361 atgctgtcga aagctatgtc tgaggggtac ctggccagat atggcgatca agccgaaagt
     3421 tatgatgcag gggctgcatt taaatatacg ctgctttttg tttttacagg cttattattt
     3481 gctacatgta aaaagaagta cattattata aatttcatga tttatatgac cctggctact
     3541 atcattatac taattggtgc acggggagca tttgtatcaa ccatactaat gggggtatgg
     3601 ttatacggtt taaataagaa aataagttta aagaaacttt ccattatttt cttgtcgttg
     3661 gccggaatac ttttaattat catgaatttt tctgcacgtg gcaattatta ttcaaatgaa
     3721 ctcatcgatt ctttattgcg tttcctctat gatcagggta atacacttgc tgtggtgggt
     3781 tatgtaagaa ccattaccga ttatccagtg ttcgcatata tttcaacatt tttgcctgga
     3841 agttcattct ttgcatcaat gtttcatggt ggaagtgatt taaatagcta catgctaagt
     3901 tttcccgctt atgcaaacta tgttgctaat ccaaatatgt attttgctgg atacggcctg
     3961 ggatggtcaa tcatcagttc tgtggaaatg ttctccgccg atatcttatt cattttttgc
     4021 attttttcgg tccttattgg tatgttttta aagtttgcta atgaacaggc aaaaaggaat
     4081 agtttctggt ttggggctgt tgcgtgtttg tgtgcgaaaa ttttatttac agctcgtgct
     4141 gatatcaaaa cgataattcc actatttatg tattatgcaa taatgtatct aattataagc
     4201 actgctatgc gaattttgag gcaaagatat ggataatagg gatgtaatgt tttcggttat
     4261 agttccatgc tataacgtgg tttcgcatat aaatgagact gtagcttcac ttaataaagc
     4321 tatcgattcg gcctgtacca cggtcgaagt catttttgtt aatgatggct ctacagataa
     4381 aacattggat gttttaaacg aaatctgcag taacaaacca tcatatcgtg ttattgatca
     4441 ggaaaatggt ggtgtctcaa acgcaaggaa tacaggaata agagagtcta cagggaaata
     4501 tattctcttt ctggattcag atgatacata caaaaataat atattttcga ttttatcgaa
     4561 catatcattt tcagatgata ttgttttctt tagctatgaa aagatagacc agagcggtaa
     4621 tacgagggtt tatactattc caaactctta tatggccgag aattcaatta ctgtattaaa
     4681 agatcttttt agtaaaaaaa tctatttaaa tatttgtgcg ctagctgtaa atagggattg
     4741 tatatttcag aataatattt ttttcgatga aagtattcaa cattgtgagg atcttcaatt
     4801 catcattaaa atgattactt attcaaaatc attccaattc ataaatgaac ctttatttac
     4861 ctataattac actcccggtt ctgctgttaa tagcaaggta gatgagaaac attattcaaa
     4921 atttattgcc tttgagaaca tgcgcgatgt ttttataaaa aatgataaat ataaagagtt
     4981 aatcccggcg tacgattttt acgttgcaac agtatactta ttactactta aaacgattgt
     5041 ctcaaatggt gtggttaaca acaaacaact aagtaagttc gttgaatatt cttttatcct
     5101 caagagaaaa atggaattac caccaaatac aatgggtgct tttgttttga tatttaaaat
     5161 aattaattat atttttccta tgaaagtaaa atttttaatg atgtcattta tcttacggat
     5221 gaataaaggt ttaagatgag tattacaaat aaaacaattg cgctggtcat tgtaacatat
     5281 aatagatgta accttttgat ggaaatgctt tcctctatcg aaaacatgag tgttaagcct
     5341 gatattgtct atgttataga caataacagt agtgataata cttcatcggt tgtcacagag
     5401 tgtgattcaa gaaaaaatat taatataaaa tatcacaaca ctggatataa cgctggaggt
     5461 gctggcggct tttatatcgg cagtaaaatg gcttatgaag atggttggga tagaatttgg
     5521 cttgcggatg atgacattgt tttggataag gagtgtctta gtaacgcaat ggagtatgat
     5581 gatggacgca ctatcttaca acctatgcgt tataatatgg acggatcctg tgccgaaatt
     5641 tcagctatcc agtacgattt aagtaatcct ttttatttac gtccaaaacg caagacggtt
     5701 cagaatattt tcaacaaaaa tattctgtca tatgatatcc aatccattcc gtttgaagga
     5761 ccgattattc ccagggaagt atttaatgtc ataggttttc cagatgaacg tttctttata
     5821 tttaatgacg atttagattt tgcgattaga gctcagagag ctggcttcag cataaaatgt
     5881 attactaacg caaaaatcgt acgtaaaatt ccctttgtac agtcggtagc attaaaaaca
     5941 tggaagggct atttcatgtt tagaaattat tttcgggttc agaaagttta tggtttaagc
     6001 ccacttattt atctgagaat tttgctcgtg ttctgccttg cactgggaca ttcactcgtg
     6061 cgtatggata tcaattcaat taagatgctg tgcggtgcgt tgaaagatgg tctctctcaa
     6121 gaatttaagc tgactgagaa atacaaacca tgagtactac cgatagccct aatataaaaa
     6181 catcaaataa caattttgat atagtaaggc tcattcttgc gttagttgtt gttttctcac
     6241 atataagaga tttaaataat attaattccc ggttcttctc cattttctca ggtgactttg
     6301 ccgtagaatg ctttttcgta attagcggtt atttgatcat tagaagcttt aaaaataatc
     6361 aaaatatcag aaaatatatt ttttcgaggg ggatgagaat tctccctatg tactacgttt
     6421 ctatagtatc ctttttcgtt atattatttt ttatctctca cctttccgtt gttgaatatt
     6481 taaaggaagg cggaattcga tatttagtat ttaatggagt attcctcaat ttcatccagc
     6541 cttcgctgcc gggagtgttc gttgataatc ctcgaatctc tgcggttaat ggggctcttt
     6601 ggactataaa agttgagatc ctattctata tatttgttcc attctttgtc ctgttcatta
     6661 aaaaaatcaa tcgacatatt attcctatat ttataattct tattttatcg tctattgtat
     6721 tcaagtggat tgttacagac tatcatcaaa ttttacatat tcctgagcaa ctggttaatc
     6781 agttaccgtc ggtgctaagt tattttctat taggcggctt ggttaattat ctggatctat
     6841 ctaagatatc aaaaaaaaca gtattttggt tttttatagt atcagctttt tatcttttac
     6901 taagtagtaa aacgtcattg gatttacttg ttcgcccatt tgttattgca gggttcatac
     6961 tgccgatatg tttgcagcgt aaatttttaa taagtgtgcc tgaaaaaatg ggtgaccttt
     7021 cgtatggtat atatgtaaca catttccctc ttgttcaatt actaattgca gtagggttgt
     7081 ataattcatt ttggttaggt ttttgtttaa catttactag tctttttatt ttgtctttta
     7141 ttagttggca tcttattgag aagccagctc ttcgttttgc taaatctgca ggaaaaaata
     7201 aagttgtgag gggtgtatga gaattgctat tgttggtgct ggatttagtg gtgcagttat
     7261 agccagggaa ttggctgaac atggtcactc agtagatatc tttgagaaaa gagaccatgt
     7321 tgctggtaat tgttatagta aaagagaccc agacacaaat gttatggttc atgtttatgg
     7381 cccgcatatt ttccatacgg atgataaaga agtatgggaa tatatcaata aattcggaat
     7441 gttcgagcca tatgttaata gagtcaaggc cataactaaa ggcgcggttt attccctgcc
     7501 aatcaatctc catacaatta accagttttt ccacaaaact ctgaaccctg ctgaagcgaa
     7561 agacttcata aattctttgt gtgaactatt cgaatttgag ccgattacat tcgagcagca
     7621 ggctttatca tttattggtc gagacttgta cgaggcattt tttagaacgt ataccattaa
     7681 acaatggggt cttgaacctt cagagcttcc tgccagtatt ttgaaacggc taccagttag
     7741 atttaattat aacgataact atttcagcca tcgttatcag ggtatgccac gtgatggata
     7801 tactaatata attagtgcca ttttggacca tgcaagtatc gctgtgaaat taaacactga
     7861 tttcaaaaaa aatgtcggca atgaatatga tcacgtgttt tatagcggca cgattgacgg
     7921 ttattttgac tactcccatg gccgccttcc ttacaggact ctagattttg tcacggaaag
     7981 acataaaggt gattatcaag gaaccgctgt aattaactat tgtgatgata gtgagaaatt
     8041 tactcgtatt actgaacaca aatatttttc cccttgggaa gagcacagca atacaataat
     8101 ctataaagaa tacagccgga catgtactga gactgattct ccctactatc caattcggca
     8161 ggttcaggaa aaagaattac taagaaagta tgttgattta gcaaatcaaa caaacaatgt
     8221 ttcatttatt gggcgtttag gtacgtacag atatcttgac atggatgtaa ctattcgtga
     8281 ggcactaaat gctgtaaaac tctttatgaa aataaaaaag gagaacgaaa aagttccagc
     8341 atttttcacg gaaatactgt gaagatataa tttctgctcc gagatacata atgtcgatag
     8401 tattagtaat ggtgagcatt gttatttatt gtcagtttga cagcactctt ggctatccag
     8461 atttgttgga tatgagttaa tatcacacca cattcaagcc gcgcatatct cgcggtgact
     8521 acacctgaca ggagtatgta atgtccaagc aacagatcgg cgtcgtcggt atggcagtg
//

</pre>