LOCUS       GU014555                9510 bp    DNA     linear   BCT 08-JUN-2010
DEFINITION  Escherichia coli strain F9884-41 O-antigen gene cluster, complete
            sequence.
ACCESSION   GU014555
VERSION     GU014555.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9510)
  AUTHORS   Wang,Q., Wang,S., Beutin,L., Cao,B., Feng,L. and Wang,L.
  TITLE     Development of a DNA microarray for detection and serotyping of
            enterotoxigenic Escherichia coli
  JOURNAL   J. Clin. Microbiol. 48 (6), 2066-2074 (2010)
   PUBMED   20351209
REFERENCE   2  (bases 1 to 9510)
  AUTHORS   Wang,Q., Wang,S., Beutin,L., Cao,B., Feng,L. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2009) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P. R. China
FEATURES             Location/Qualifiers
     source          1..9510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="F9884-41"
                     /db_xref="taxon:562"
                     /note="serogroup: O27"
     misc_feature    1..9510
                     /note="O-antigen gene cluster"
     gene            899..1639
                     /gene="wejU"
     CDS             899..1639
                     /gene="wejU"
                     /note="glycosyl transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WejU"
                     /protein_id="ADI43270.1"
                     /translation="MSFNETFSIIMPAHNAEATIEYSINSVLNQTDQDFQLIIVNDAS
                     TDNTQKIIDRYNDERIIKLVNKVNLGVAETRNRALNIATGRYIAFLDSDDVWRRNKLE
                     RQRREFNNGHLLVCSAYCIMDNDGTVNDKKIFFKENIEYKDMLKSNFIANLTGCYDSQ
                     MLGKFYQKKIGHEDYVMWLDILSVAKVAYCIPECLAVYRIANHSVSSNKIRAMHWQFN
                     IYRQYLKMNIAKAVYYFIHYAINALKKR"
     gene            1672..2904
                     /gene="wzy"
     CDS             1672..2904
                     /gene="wzy"
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ADI43271.1"
                     /translation="MKINKNSVFISCIFILNPLASVLCALRSINKKNLGFVIVLISVL
                     TFFITLYTPPYQDLYRRYISTYYIYNSQTTLWEALENKVDFLFYLCSWLFFKLDFPFY
                     LIPALFSSISCYCILSAANDFWRYDKKNVSRYILLIAFLCIFSIIDVIMIASTLRFGF
                     AVALFIKGISTYYVVGKKKRAYAFFLLATSCHVSMLLPVCVIFVNKFIKISWLNCFIL
                     SILMYISSIYLVPFILNIVNLGHLNEYVTTGYINTEYANLSNNTNTLLVQAYKWLIFF
                     VFLIFFIKSRTLFPEFDNYVRLLIVFSAMTALSITIFNRYFIGLISPLVFIGGVSCLS
                     RLSFKPLFQMIIIITLLFNILVINIFLERRQIIMAKMWRGLYLPPAFSLLYSMRDFDN
                     YLKEIDNDGNWVKNRLAE"
     gene            2952..3767
                     /gene="wejV"
     CDS             2952..3767
                     /gene="wejV"
                     /note="glycosyl transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WejV"
                     /protein_id="ADI43272.1"
                     /translation="MVVKNEVDIIEYTLEQALKWADYILVLDNNSTDGTTDVIKKMSE
                     LEQKIIFWGGYGGPFRDSLRAVIYNDYYYLCNDGDWWCRLDADEVYIDNPREFLSSMD
                     ESIDHVYSASFQYYLTKRMLENEEQNTRKFDISNYKYFKCNWSEIRFIKIKKSTIWPL
                     HFANPLFLLKPSSRRIRLKHFQYRNSKQMEKRFRDRSPEEGKNLFSHELKQNKVWDET
                     DLTYDQGLYEYDDEILPKIKKISTFRKVIFSCIVYGLKKFSLERILLPTKEKV"
     gene            3772..4665
                     /gene="wejW"
     CDS             3772..4665
                     /gene="wejW"
                     /note="glycosyl transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WejW"
                     /protein_id="ADI43273.1"
                     /translation="MEKIKILPLVSVYITTKNRPVFLERALKSVVNQTYKKIEILICN
                     DGSDDCYDKDYNAVINKYTTLFQNINIEYRKNIESLGACESRNLLIENARGVFITGLD
                     DDDYFFPDRIKLFVENYDDKYAFLCANGSLNNDCLNKKTIDGGKVITFEEMKNYNLIG
                     NQIFIRRERLIDIGGFDKNMPAWQDYDTWFRLLKKYYCCYKLNARTMYIDTDLSRHRI
                     STTSKAYLGYQAFIRKHSSDLNAYNLLSLKYNDLINRKEKFPILKFELLRKPFLFIRL
                     AKERSVYYYPHIYIIYRKIFK"
     gene            4680..5966
                     /gene="wzx"
     CDS             4680..5966
                     /gene="wzx"
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ADI43274.1"
                     /translation="MKNYLKITINNKVFSNSIWMILEKIISVFGLLFVTSYVAKYIGP
                     SNFGILTYVASIFIIAQALSTFGSEHVLFKRIAKNRKSGINLMMSTSYLRHLVFISLS
                     IGILCYLWVSSDQLTFTFGVATAVAYYFSAIDVFSVYNNAVLNSKLNTYCNITGLLIS
                     LIVRYIIAHYELDIRYLCIPIILVTCIPFAMKVYLYHSKKFNFQPRIDKKRYGKYLIK
                     TGTPLAFSTVSAIIYTRVTLFFIMAYCGPSELGIFSVASQLATSWMFITSAFITSYFT
                     KIYTEKDESIALIQAAKLNGSVLFCAIIVILILVLIGKKLILTLYGVAYLQAYDVMLI
                     LCINTLFSCMGPVASKFIIFKSGFSFLSLKMMLTMLLSIPSAYFLTKEYGIYGAAIST
                     IITEFLSLTLMNYLFRNGIIFKLHYKMFFIKTYARN"
     gene            6002..7039
                     /gene="wejX"
     CDS             6002..7039
                     /gene="wejX"
                     /note="glycosyl transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WejX"
                     /protein_id="ADI43275.1"
                     /translation="MKEKNIAIVIYDAYGYAGTENVANFMSECFSTKHKVTVFSLEGS
                     GKTFYPFHNVINIISLSGFRFKLFRLMSLMKKEKYDAVFVISMGKLSVLYSLYSFLTG
                     LTSRSIACEHIALSSFPKPIKFLKFIFLRRFERVVVLTKTDEIKYINHKIRAVHIPNP
                     LNYNHFMRNSRSNIYLAVGRLEQQKGFDRLLKIWCTFYKNNESAKLFIAGEGSQKDKL
                     HCLCKELQISQSVIFLGKVDNIDRYYKKADVLLMTSYYEGMPLVLLEAKSWSLPVIAY
                     DCPTGPREIIANNIDGFLIPDNDHELFISKMIELTSNDTLYYEFCKNTAITSRSFNAN
                     AACQKWNNLIE"
     gene            7045..8067
                     /gene="wejY"
     CDS             7045..8067
                     /gene="wejY"
                     /note="O-actetyl transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WejY"
                     /protein_id="ADI43276.1"
                     /translation="MERSFRLDLMRLLGLLLIIFAHIGPPNALFQIRTFDVPMMIFVS
                     GVAYYISSPSFFSWKIYYIKRVSRLLFPVWCFFALFYGVVYLFDIKVFSEYLKWNMIF
                     STFLLNGFGYLWIIRIFIIIAILAPFIVMFFRTKTFYFDVSVIILSIFATTIADYYFI
                     HMNNKYARYIVVDILLPALTYSSIFILGYKWHAYTRFDKNTVGFVSLIFLIVAQVYML
                     FEQQGIIQPQNFKYPPTIIYISFSIVMIVILDFILKSCFDNTASSKYKCIIFLSSNTI
                     WVYLWHIPIVEYFKLTDDKLEWYVKYAIVVCISFLITFLQVYIIKWIVRQNPCKLKFL
                     KVIFTG"
ORIGIN      
        1 attgtggctg cagggatcaa agaaatcccc ctggtaactc atgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacttc ttatgaatta gaatcactcc ttgaacagcg tgtgaagcgc
      121 cagctgctgg cggaagtaca gtccatctgc ccgccgggcg tgacaattat gaacgtgcgt
      181 cagggtgaac ctttgggatt aggtcactcc attttgtgtg cacgacccgc cattggtgac
      241 aatccgtttg tcgtggtgct gccagacgtt gtgatcgacg acgccagcgc tgatccgctg
      301 cgctacaacc ttgctgccat gattgcgcgc ttcaatgaaa cgggccgtag ccaggtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactctgtca tccagaccaa agagccgctg
      421 gaccgcgaag gtaaagtaag ccgcattgtc gaattcatcg aaaaaccgga tcagccgcag
      481 acgctggact cagacatcat ggccgttggt cgctatgtgc tttctgccga tatttggccg
      541 gaactagaac gtactcagcc tggtgcatgg ggacgtattc agctgactga tgccattgcc
      601 gagctggcga aaaaacagtc cgttgatgca atgctgatga ccggcgacag ctacgactgc
      661 ggtaaaaaaa tgggctatat gcaggcgttt gtgaagtatg ggctgcgcaa cctgaaagaa
      721 ggtattaagt ttagagaaaa aattttaaag ttactttaat tttcatctta tctacggggg
      781 tattgatatc ctcgtacttt tttataaacg tttatagata gcgctttggt tgctgtgagc
      841 caagggcggt agcgttctat ttttaatgaa atattacaga ggttaataga gtagagaaat
      901 gagctttaat gaaacattct caattataat gcctgcacac aatgcagaag cgactataga
      961 atatagtata aattcagttc tcaaccaaac tgatcaagat tttcaattaa ttatagttaa
     1021 tgatgcatcg accgacaaca ctcaaaaaat aattgatagg tataatgatg agagaattat
     1081 aaaattagtc aataaggtga atttgggagt ggcagaaaca cggaatcgtg cactaaatat
     1141 tgctaccgga cggtatattg catttttgga tagtgatgat gtctggagaa gaaataagtt
     1201 agaacgtcag agaagagaat ttaataacgg tcacttatta gtttgctctg cttattgcat
     1261 tatggataat gatggaactg taaatgacaa aaaaatattt tttaaagaaa atatagaata
     1321 taaggatatg ttgaaatcaa atttcattgc aaatttgact ggatgttatg acagtcagat
     1381 gctaggtaag ttttatcaaa aaaaaatagg tcatgaagat tatgttatgt ggttggatat
     1441 cctcagtgtg gctaaagtag catattgcat accagaatgt ttggctgtat accgtatagc
     1501 gaatcattcg gtttcatcaa ataaaattcg agcaatgcat tggcaattta atatttatag
     1561 acagtattta aaaatgaata ttgcgaaagc agtttattac tttattcatt atgcaattaa
     1621 tgcgttgaaa aaaaggtagt tatttatgta tgggcggatg ttaatcgaat tatgaagata
     1681 aacaaaaata gcgtgttcat tagttgtata ttcatattaa atccattggc ttctgtttta
     1741 tgtgcattaa gatcaataaa caaaaaaaat ttaggctttg ttattgtatt aatttcagtt
     1801 ttgactttct ttataacttt gtatacacca ccttatcagg atttgtatag gcgatatata
     1861 tccacatatt acatatataa ttcacaaaca accctatggg aagctctgga gaataaagtt
     1921 gattttttat tctatttatg ttcatggttg ttttttaagc ttgatttccc tttttacctg
     1981 attccggcat tatttagttc aatcagctgc tattgtatcc tttctgcagc gaatgatttt
     2041 tggcggtatg ataaaaaaaa tgttagtcgt tacatcctgt taattgcctt tctatgcatt
     2101 ttttcaatta tagatgttat tatgatagca tctacattga gatttggttt tgctgttgct
     2161 ctgttcataa aaggcattag cacttattat gtcgtcggta aaaagaaaag agcatatgct
     2221 ttttttctct tagcaacttc ttgtcatgta tcgatgttgt tgcctgtgtg tgtaatattt
     2281 gtcaataaat ttataaagat atcgtggtta aattgtttta ttttaagtat tctaatgtac
     2341 ataagtagca tttatttagt tccatttata ttgaatattg taaatttggg acatttaaat
     2401 gagtatgtaa ccactggcta tataaatact gaatatgcaa atttatcaaa taatactaat
     2461 acacttttag ttcaggccta taagtggttg atattttttg tgtttttgat tttttttatt
     2521 aaaagtagga cgctatttcc tgaatttgat aattatgtca gattgcttat tgtctttagc
     2581 gcaatgacgg cgttatcaat aacaatattt aatcgatatt ttattggcct tatatcacca
     2641 ctggtattta ttgggggggt gagttgtctt agccgtttaa gcttcaaacc tttatttcag
     2701 atgataatta ttataacatt actttttaat attcttgtca ttaacatctt tttggaaaga
     2761 cgacaaatta taatggcaaa aatgtggcgt ggtttatacc tgcctccagc attttcttta
     2821 ttatatagca tgagagattt cgataattat cttaaagaaa tagacaacga tggtaattgg
     2881 gtgaaaaata gactcgctga atgaaatgta tttaaaagga gctcttcttg aataataaaa
     2941 taattgcctg catggtagtt aaaaatgaag ttgatataat tgagtataca cttgagcagg
     3001 cattaaaatg ggctgattat attcttgttt tagataataa tagtactgat ggtactactg
     3061 atgtgataaa aaaaatgagt gaacttgagc aaaaaattat tttttggggg ggctatggag
     3121 gaccatttag ggattcgctc agagcagtta tctataatga ctattattat ttatgtaatg
     3181 atggtgattg gtggtgtaga ttagatgctg atgaggtcta tattgacaat cctagggaat
     3241 ttctatcatc tatggatgaa agcatagacc atgtttattc agcaagcttt cagtattatc
     3301 ttacgaaaag aatgttagaa aatgaagaac agaatactag aaaatttgat atatctaatt
     3361 ataaatattt taaatgtaat tggtctgaaa taaggtttat caaaataaaa aaatcaacaa
     3421 tatggccgct acacttcgca aatccacttt ttctgttaaa gcctagttcg cgtagaatta
     3481 gacttaaaca tttccagtat aggaattcaa agcaaatgga aaaaagattt agagatcgtt
     3541 cgccggaaga agggaaaaat ttattttcgc atgaattaaa acaaaataaa gtttgggatg
     3601 aaacagattt aacatatgat cagggattat atgaatatga tgatgaaata ttgcccaaga
     3661 taaaaaaaat atctactttt agaaaagtta ttttttcttg tattgtttat ggtttgaaaa
     3721 aattttcttt agaaagaatt ttactgccca caaaagaaaa agtgtaataa aatggaaaag
     3781 attaagatat tacctttggt ttctgtatat attactacta aaaacagacc tgtctttcta
     3841 gaaagagcat taaaatctgt agttaatcaa acatacaaaa agatcgagat tcttatttgt
     3901 aacgatggtt ctgatgattg ttatgataaa gattacaatg ctgtaattaa taaatataca
     3961 actctttttc aaaatattaa tattgaatat cgtaaaaata tagaatcact cggagcatgt
     4021 gagtcacgta atctattaat tgaaaatgca agaggtgttt ttattacagg attagatgat
     4081 gatgattatt tttttcctga cagaataaaa ttatttgttg aaaactatga tgataagtac
     4141 gcatttttat gtgcgaacgg aagtttaaat aatgattgct taaacaaaaa aacaatagat
     4201 ggaggaaaag taataacttt tgaggaaatg aaaaattata atttgatagg aaatcagatt
     4261 tttattcggc gggagcgatt aattgatatt ggcggcttcg acaaaaacat gccagcctgg
     4321 caagattatg atacatggtt taggttatta aaaaaatatt attgttgcta taaactcaat
     4381 gcgcgtacaa tgtatattga tacagattta tccagacata gaatcagtac aacgagcaag
     4441 gcataccttg gatatcaagc gtttattaga aaacattcat ctgatttaaa tgcatataat
     4501 ttattgagtt taaaatacaa tgatttaata aataggaaag aaaaatttcc aattcttaaa
     4561 tttgaattat tgagaaaacc ttttcttttt attaggttgg ctaaagaaag gtctgtctat
     4621 tattacccac atatttatat tatttaccga aaaatattta aataaagaga aagggagata
     4681 tgaaaaatta tttaaaaatt acgattaata ataaagtgtt ttcaaattca atatggatga
     4741 tattagaaaa aataatatct gtatttggct tgctctttgt tacatcttat gtggcaaaat
     4801 atataggccc ttcaaatttt ggtatattaa catatgtagc ttcgattttt attatagccc
     4861 aagcgttatc tacttttgga agcgaacatg ttttattcaa aaggattgca aaaaatagga
     4921 agtccgggat taatttaatg atgtcaacaa gctatctaag acatctggtt tttattagtt
     4981 tatctatcgg gatcctgtgc tatttatggg ttagttctga tcaattaacc tttacatttg
     5041 gcgtcgcaac agcagttgca tattatttct cagcaattga tgttttttct gtatataata
     5101 atgccgtatt aaattcaaaa ctaaatacgt actgtaatat cacaggctta cttataagtc
     5161 ttattgttag atatataata gcgcattatg agttggatat tagatatttg tgcataccga
     5221 ttatacttgt aacatgtatt ccgtttgcaa tgaaagtata tttatatcat agcaaaaaat
     5281 ttaattttca acctagaatt gataaaaaaa gatatggtaa atatctaatt aagacaggta
     5341 cacctttagc gttttcaaca gtttcagcta ttatttatac aagagttacc ttgtttttta
     5401 taatggcata ttgtggacct agtgagctcg gcatattctc tgtagcaagc cagttagcaa
     5461 catcgtggat gtttataaca tctgctttta ttacttcata tttcaccaaa atttatactg
     5521 agaaagatga gtctattgca ttaatacaag cagcaaagct aaacggaagt gtactatttt
     5581 gtgcaataat tgtgatttta atattagttt taattggtaa gaaattaatc ttaacattat
     5641 atggagtcgc ctatctgcag gcatatgatg taatgcttat tttatgtatc aatacattat
     5701 tttcttgtat gggacctgta gctagtaaat ttataatatt taaatctggg ttttcttttt
     5761 tatcactgaa aatgatgcta acaatgcttt tatcaatacc ttctgcgtat tttctaacaa
     5821 aagaatatgg tatttatggt gcggctatta gtacaataat cacggaattt ttatccttaa
     5881 cattaatgaa ttatctattt agaaatggca ttatatttaa attgcattat aaaatgtttt
     5941 ttattaaaac atatgcgaga aattaatctt atcttaatta ctaaatataa atggtataaa
     6001 agtgaaagaa aaaaatatag ctatcgttat ctatgatgct tatggttacg ctggaacaga
     6061 aaatgttgca aactttatgt ctgagtgttt ttccacaaaa cataaagtta ctgttttctc
     6121 actagaaggc agtggaaaga cattttaccc atttcacaat gttataaata taataagcct
     6181 ttcaggtttt cgatttaagt tgtttcggct aatgagttta atgaagaaag aaaaatatga
     6241 tgcagttttt gttattagca tggggaaatt gagtgttttg tattcactat attcattttt
     6301 gacagggttg acttccagat caattgcatg cgaacacatt gcattgagct catttcctaa
     6361 gccaattaaa tttttgaagt ttatcttttt acgtaggttt gaaagagttg ttgtattaac
     6421 aaaaactgat gaaattaaat atattaacca taagattaga gctgttcata ttcctaatcc
     6481 attaaattac aatcacttca tgcgaaattc cagaagtaac atttacttgg cggtcggtag
     6541 attagagcaa caaaagggat ttgacaggtt attaaaaata tggtgtacct tttataagaa
     6601 taatgaaagt gctaaacttt ttattgcagg tgaaggtagt caaaaggata aattacattg
     6661 tttgtgcaaa gaattacaga tatcacaatc tgtaattttt cttggtaaag ttgataatat
     6721 tgatagatac tacaagaaag ctgatgtgtt attaatgact tcatattatg aaggaatgcc
     6781 tttggtttta ttagaggcca aatcttggtc attacctgtt attgcttatg attgtcctac
     6841 tgggccacga gaaataattg cgaataatat tgatggtttc cttataccag ataatgatca
     6901 cgagttattt atttcaaaaa tgattgagtt aacctctaat gatacccttt attatgagtt
     6961 ttgtaaaaac acagctataa catctagaag ttttaatgca aatgctgctt gtcaaaaatg
     7021 gaacaattta atagagtgaa aataatggaa agaagttttc gtctggattt aatgagatta
     7081 cttgggctgt tgttaataat ttttgctcat atagggccac ctaatgcctt atttcagata
     7141 agaacatttg atgtaccaat gatgattttt gtttctggag tagcttatta tatatcgagt
     7201 ccctcttttt tttcttggaa gatctattat atcaagagag tttcaagatt attatttcct
     7261 gtttggtgtt tttttgctct tttttatgga gtggtgtatc tttttgatat taaagtattt
     7321 tcagagtatt taaaatggaa tatgatattc tcaacttttt tactaaatgg atttggctat
     7381 ttatggataa tacgaatttt tataattatt gcaatattag cacctttcat agtgatgttt
     7441 tttcggacaa aaacatttta ttttgacgtg tctgttataa tattatctat atttgcaacg
     7501 acaatagcag attattattt tattcacatg aataataaat atgcaagata cattgttgtt
     7561 gatatattac ttccagcatt aacttattca tctatattta ttcttggata taaatggcat
     7621 gcctatacta gattcgataa aaacacagta ggctttgtgt ctttaatttt tcttatagtc
     7681 gcccaagttt atatgttatt tgaacaacag ggtattattc agccacagaa ttttaaatac
     7741 ccgcctacaa taatatatat atcgttctct attgtgatga ttgttatttt ggattttata
     7801 ttgaaatctt gttttgataa tactgcgtca tcaaaatata aatgtattat ttttttatcc
     7861 agtaatacaa tatgggtata cttgtggcat attccaatag ttgagtattt caaattgact
     7921 gacgataaac ttgaatggta tgtaaaatat gcaatcgtag tttgtatttc ttttttaatt
     7981 acttttttac aagtttatat tattaaatgg attgttaggc aaaatccatg caaacttaaa
     8041 ttcttaaaag taatttttac tggatgatat ttcatttttg ggtgttttat tcgttattgt
     8101 acattatagt taatttcttt aatatctagc gatatatcaa ttaatgacta acccctgaca
     8161 ggagtaaaca atgtcaaagc aacagattgg cgtcgtcggt atggcagtga tggggcgcaa
     8221 ccttgcgctc aatatcgaaa gccgtggtta taccgtctct attttcaacc gttcccgtga
     8281 aaagacggaa gaagtgattg ccgaaaatcc aggcaagaaa ctggttcctt actatacggt
     8341 gaaagagttt gttgaatctc tggaaacgcc tcgtcgcatc ctgttaatgg tgaaagcagg
     8401 tgcaggcacg gatgctgcta ttgattctct caagccatac ctcgataaag gcgacatcat
     8461 cattgatggt ggtaacacct tcttccagga caccatccgt cgtaaccgtg agctttctgc
     8521 agaaggcttt aacttcatcg gtaccggtgt ttccggaggc gaagaaggtg cgctgaaagg
     8581 tccttccatt atgcctggcg ggcagaaaga agcctatgaa ctggttgctc cgatcctgac
     8641 caaaatcgcc gccgttgctg aagatggcca accgtgtgtt acctatattg gtgccgatgg
     8701 cgcgggtcac tatgtgaaga tggttcacaa cggtattgaa tacggtgata tgcaactgat
     8761 tgctgaagcc tattctctcc tgaaaggcgg cctgaatctc tctaacgaag aactggcaca
     8821 gacctttacc gagtggaata acggtgaact gagtagctac ctgatcgaca tcaccaaaga
     8881 catcttcacc aaaaaagatg aagacggtaa ctacctggtt gatgtgattc tggatgaagc
     8941 agcaaacaaa ggtacgggca aatggaccag ccagagtgcg ctggatctcg gtgaaccact
     9001 gtcgcttatt accgagtctg tgtttgcacg ttatatctcg tctctgaaag atcagcgcgt
     9061 tgccgcatct aaagttctct ctggcccgca agcgcagcca gctggcgaca aggctgagtt
     9121 catcgaaaaa gttcgtcgtg ctctgtatct gggcaaaatc gtttcttacg ctcagggctt
     9181 ctcgcagctg cgtgctgcgt ctgaagagta caactgggat ctgaactacg gcgaaatcgc
     9241 gaagattttc cgtgctggtt gcatcatccg tgcgcagttc ctgcagaaaa tcaccgatgc
     9301 atatgccgaa aatccgcaga tcgctaacct gctgctggct ccgtacttca aacaaattgc
     9361 cgatgactac cagcaggcgc tgcgtgatgt cgttgcttat gcagtacaga acggtatccc
     9421 ggttccgacc ttcgccgctg cggttgccta ttacgatagc taccgtgccg ctgttcttcc
     9481 tgcgaacata atccaggctc agcgcgacta
//