LOCUS       KJ755562               16452 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain E 41a serotype O24:H- O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ755562
VERSION     KJ755562.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16452)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16452)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Baranzoni,G.M.,
            Liu,Y. and Debroy,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E 41a"
                     /serotype="O24:H-"
                     /db_xref="taxon:562"
     misc_feature    1..16452
                     /note="O-antigen gene cluster"
     gene            74..853
                     /gene="wfgD"
     CDS             74..853
                     /gene="wfgD"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-Glc:alpha-D-GlcNAc-diphosphoundecaprenol
                     beta-1,3-glucosyltransferase"
                     /protein_id="AIG62578.1"
                     /translation="MNKETVSVIMPVYNGARTIISSVESIINQSYQDFVLYIIDDCST
                     DDTFSLIFNRYKNNQKIRMLRNDSNLGVAESRNVGIKAATGKYISFCDADDLWHQNKL
                     ERQIELFNNECVDVVCSNYYVVNINGDVIGEVKAPHIITYRKMLMKNHIGNLTGIYNA
                     NKLGKFYQKNIGHEDYLMWMEIINKTNVAICIQDNLAYYMRTSNSLSANKVKTAKWMW
                     NIYRQCLHLSFTKSLYCFFMYAFNGVIKEIMHLPLKALRKK"
     gene            2562..3182
                     /gene="pglD"
     CDS             2562..3182
                     /gene="pglD"
                     /EC_number="2.3.1.203"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylbacillosamine N-acetyltransferase"
                     /protein_id="AIG62579.1"
                     /translation="MKKKLIIIGAGGFAKAVIDSLDHACYEIHGFIDSFKCGEHQGYK
                     IISRTIDSLDEPHKYCYFIAIGEPYYRAIWLETINAMKLETINVIDRTAIISKGAILG
                     SCIYVGKMAIVNCDSHLEDGVVINTRALVEHGNYISYCSNISTNVVLNGDVFVGRKCF
                     IGSCTVVNGQLKIGDSSIVGSGSVVIRNVDENVVVAGSPTRLIRKR"
     gene            3187..4224
                     /gene="nnaB"
     CDS             3187..4224
                     /gene="nnaB"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl neuramic acid synthetase"
                     /protein_id="AIG62580.1"
                     /translation="MSSVYIVAEIGCNHNGDYNLAKLMIDEAYSAGVDAVKFQTFKAD
                     SLISKYAPKAEYQIKTTGNSESQLEMTRKLELSCEEYIKLEEYSKSLGLDVFSTPFDL
                     ESIEFLYTRKQTTWKIPSGELLNLPYLEKIALLPGDKKIILSTGMATLAEIKQALKIF
                     TDNGVKAEDITILHCNTEYPTPYEDVNLNVLAGLKKIFQEYNIGFSDHSPGYFAGIAA
                     VPYGITFIEKHFTLNKDFEGPDHKASITPDELRLLCNGIRAIERALGSFDKRVTNSER
                     KNKLVARKSIVAKRDIKKGEIFTTDNITTKRPGNGISPMYWYEVLGKKAEQDFLEDQL
                     IKVSGFEEQEI"
     gene            4227..5486
                     /gene="nnaC"
     CDS             4227..5486
                     /gene="nnaC"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="AIG62581.1"
                     /translation="MRKKIAIIPARSGSKGLPNKNILMLFDKPLIAYTIEAAIKSELF
                     EKVIVSTDSCEYKDIALRHGAEVIMRDKSLASDTATSYMVVKDVLEKNISCDYFVLLQ
                     PTSPFRNYKHIREAVEKFENTDDIDFLVSMVKTNKSADLINQLAPNDNLKYFNADFAN
                     YRRQNQENYCPNGAIFIGNVDAYLRQKHFFGERSIAYVMDRIDSLDIDDKLDFEFAIS
                     LMLKKNRMKDLQKEILKRIKEKEFFFDKIKPVTLVGHSIIDYWNVTHLNGREVNNLGI
                     AGINSKEYYDLIIKNNIIKFGDTVILMLGTNDIVVDDWSVDFTILWIEKIVKHIKKIN
                     HNASIYILSIPPVLGRIDRNNETIRKLNSELSKFFENYQNVIFITLSKVFYDQYGNLK
                     ENFTYDGLHFSQLAYNQLTKEIEEKIK"
     gene            5483..6670
                     /gene="phaR"
     CDS             5483..6670
                     /gene="phaR"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor"
                     /protein_id="AIG62582.1"
                     /translation="MKKILYITGSRAEYGIMKRLLRKLKDNEHIELSIIATGMHCDAK
                     YGYTYQNIIDDGFEVKELFDINIESSTNSGIISTMSRAQNLFGAYFEEYKYDAIIILG
                     DRYEMLSVAIAASIHGIPIIHLHGGEQTLGNYDEFIRHCITKMSHLHLVATEKYRERV
                     IQLGELPSWVINIGAMGAENTVSLSIPEHKELQSKYENSLSNYFMILFHPETLTKNKS
                     VHDQIKELIKALDIAHQKYHCNFIFIGTNADTNSDIITKEILTFCESYNFPYLLSLPT
                     QDYLGLCKYSLGLIGNSSSGLIEIPSLGVPTINIGSRQAGRERGKSVIDVECCYTDIL
                     EKIDDVISNRIDRTYYNPYYKENSAEIAMSSIISFLDWISDNKNRIKKFYDIPKVTTL
                     INS"
     gene            8358..9365
                     /gene="wfaR"
     CDS             8358..9365
                     /gene="wfaR"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="AIG62583.1"
                     /translation="MKSNVYICKTTWEIFVSILLASDIYYDKKIKSVFVIEVAEENIN
                     YLSKLSDFFFVSKVIPVRFSSNKYVKALCFHYRVRYYLPKMLGEYLKSDVRVNLFIDQ
                     GVFGQYFIKNNKDVYLYEHGNGNYCVGPYPNYKMIKKFLGITEGYGRHSRVKRIYLQH
                     PEKAPKDIIHKVTGFSIKNLYNKLFLKEQQNLLSFFGIHKINKTSDVIILTQPISEDG
                     FYSEREKIEIYREIIDSRFPSDKSRVVIKPHPRDKTNYGKYIPDVMLLPRSFPMEILN
                     FTNIRFIKAITICSSAVYNFEYDISVDYIGTLRYPKLIKALGRLPAQYVHKDNYKNIQ
                     Y"
     gene            9389..10441
                     /gene="wzy"
     CDS             9389..10441
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AIG62584.1"
                     /translation="MIPYVMFYCSSLVLVVLSLVLKKYDWFWLLFLLFFSAVFVGLRV
                     DVGADYTEYAQIYNQSGNITNFELGFDIIFNYGKRLGYDYVFVSLFFFLLTTLFFIYS
                     IKELNYKTLIYFCFLLFMFVPLTSTIRQGLAIPFFVMCILNSDRPKVYFTSIALGCLF
                     HYSILFMFFFFWVRHIKQSYCRAFLIVLLFSLLSIFNIVEYIIMLLNYMPGLGAISTK
                     LLMYTQRYQVEFSMNSFVYKLAGLLLITMFMPFIKVNEKLRCSYNLYFITVCIFILLK
                     DNAVLTNRLTYATNISLVTFFSLLLSMIRGFYKKAIVSILFVFYFSFSYFKFTATDLR
                     HSVESAYMPYKSLLIN"
     gene            10457..11650
                     /gene="wzx"
     CDS             10457..11650
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AIG62585.1"
                     /translation="MKLLKSRFSYFATELINKCVPFFLLPYVSSAVGVEVYGKIELFT
                     TNYIILSFLFALGVEGWMNAHYFKMAESDFITVLSSFYLLSFLIFVICMAISLPYSYY
                     CSALLFVGYVQSLLNITTLHLRLKGKYLNAGFLLLLSSIVNAVMVYASFDWFGKTYES
                     RINSFLFSSIIILVFIVFLSIRDKNIKIKIDFSFLNRDILLFCLPLCLTMIINWVKGN
                     IDKYFIANLLDLKSLGIYAVAYQLASVINVVGIILNRTLQADLLKSMADGFYQTKKII
                     FTFLFILCVFFFLYIVAVYCGFSYVFSIDYSSSRNIALLLMIGFLLLSFSSFLINFLL
                     FYRRPRLILFQTFLITMIHVMLSFILIKKYALLGVLLTQFVTSLLTFVSTLLVCHILT
                     KSSQK"
     CDS             12108..12527
                     /codon_start=1
                     /transl_table=11
                     /product="transportase"
                     /protein_id="AIG62586.1"
                     /translation="MVTWVELSFEKKSAVLDQQGVAQKNPFVIDTVTQMCKNRLPLNI
                     ILTTTAVWHLPAEFWVIPVPMCSLAGLMNVTPTDDVYLPVRPVRIRSLNQKSNVKLSR
                     HSVHDEYQTVKLPDVLVPAVLYCINGKMKLSVTVFTK"
     gene            13455..14198
                     /gene="ybfD"
     CDS             13455..14198
                     /gene="ybfD"
                     /codon_start=1
                     /transl_table=11
                     /product="H repeat-associated protein"
                     /protein_id="AIG62587.1"
                     /translation="MDTYIRWYNERRIKLLLVQSAQKCTVNNVAGIIKQFRKSSSSKK
                     LIEHISINTSAGKVEHKLSKILLLTIFAVILGAEGWEDIEYFGETPLDFLKQYGDFEN
                     GITVHDTIARVYPVSVLQNFTSALLTGCVTTILQMIKTSSQLMEKHSGILMTRVAARE
                     RFMLLVCSQQCTVWSSDRLKRIRNPMRLLLSLLFLICWLLKEKLSHQIRWVARKILQR
                     RYKNREMIIYSLQTEIRVGLIKILRGNFH"
     gene            14510..14764
                     /gene="yhhl"
     CDS             14510..14764
                     /gene="yhhl"
                     /codon_start=1
                     /transl_table=11
                     /product="H repeat-associated protein"
                     /protein_id="AIG62588.1"
                     /translation="MNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRK
                     ATMDRNYLASVLAGKGLSLSCPDYIPSLKDPRVVTIQSEG"
     gene            15233..16303
                     /gene="pglH"
     CDS             15233..16303
                     /gene="pglH"
                     /EC_number="2.4.1.292"
                     /codon_start=1
                     /transl_table=11
                     /product="galNAc-alpha-(1->Feature
                     gb|4)-GalNAc-alpha-(1->Feature
                     gb|3)-diNAcBac-PP-undecaprenol
                     alpha-1,4-N-acetyl-D-galactosaminyltransferase"
                     /protein_id="AIG62589.1"
                     /translation="MKKITFVIADITFVGGIERVNTLLANKFFSEGYNVEIISLYNTN
                     KKVNYELNNCIKVNFVKDNSYSGAPGSFGRLKQHISSQFALFKAIKNSNADFYIVNSF
                     PMAFLSFFNVFSNKKYVVIEHVHYDYYGVAIKFFRNLLYKFYYKVVCVNESDLSKFSK
                     HLNNAVKINNPLSFSSSEITCLSEKKIIAVGRLEYQKGFDILIDIFARVNKEHPGWEL
                     HIYGVGTCEEFLRDKINQYKLNNIKLMGCVDNIQLYYPKYSVFVFSSRFEGFGMVLLE
                     AMECGLPCISFDCPTGPSEILGNGQYGILVENGNVTKFSAELADLMSDKEKKIKLSNI
                     SKTRAKEFHIDKIFSEWRKLIN"
ORIGIN      
        1 cactggtagc tgttaagcca ggggcggtag cgtgcttaag taatgttaat gaaagcatat
       61 aagaaatttt caaatgaata aagaaactgt ttcagttatt atgcctgtct ataatggggc
      121 tcgaactata atttcatcag tagaatcgat tataaatcaa tcatatcaag actttgtttt
      181 atatatcatt gacgattgta gcaccgatga tacattttca ttaattttta atcgatataa
      241 aaacaatcaa aaaataagga tgttgcggaa tgactctaat ttaggtgtgg cggaaagtag
      301 aaatgttgga ataaaagcag caacaggaaa atatatttct ttttgtgatg cggatgattt
      361 atggcaccag aataaattag aacgacaaat cgaattattc aacaatgaat gtgttgatgt
      421 ggtatgttct aattattatg tggtgaatat taatggggat gttattggtg aggttaaagc
      481 acctcatatc ataacttaca gaaaaatgct tatgaaaaat catataggga atttaactgg
      541 tatttataat gctaacaaat tgggtaaatt ttatcaaaaa aatattggcc acgaggatta
      601 tttaatgtgg atggagataa taaacaaaac aaacgttgcc atttgtattc aagacaatct
      661 cgcttattac atgcggacaa gtaattcact ctctgctaat aaagtgaaga ctgcaaaatg
      721 gatgtggaat atatatagac aatgcttaca tttatctttt actaaatctt tatattgttt
      781 ttttatgtat gcttttaatg gggttattaa ggagataatg catttaccat tgaaagcctt
      841 aagaaaaaag taataaaaag gtgaggttga tgttttgatt ttgttttaca ctttaccgcc
      901 acctgcatat atttatataa aatcaataac tagctctttg attgtatctc tgatgttttt
      961 aacttactac tttatctgcc attattaata aaaaagaagt ctttatcgga aacctagaac
     1021 gaaaaattag atgttttttt tgttacgata aagaactact gtaggtgatt ttatttcttt
     1081 aggaatgggt ttttatattc tgattatgct acaaaaagtg tttctatcag aggaaaataa
     1141 ttaaaagtag gttcttatgc taagcgaggt acattatata ttcaagggtg aatatgccct
     1201 gattgtttga aactgttttt cgcactttat atataaataa aaccttctta tataaataag
     1261 gagtgcgaaa tgggatttga aaaattgtag gtggagtttc cagagtggca taggatatat
     1321 ggtagtaact cttccctaaa taactgttca gaatagtaga gcactcaggg gggaactcag
     1381 tccggtttat gcgatcctat caatcgctga atatcccaaa tcatcaactg gattgagcaa
     1441 tgccggttat agcattaatt cccagaacta aacgacgcct gatttagtaa acgctccata
     1501 aaacaaggat aaaaaccacg cccggcggct cacagccatg ctaatgctgc attgtggtga
     1561 cagcgtcagc catgttgcca gcacagcttt gctgtgtccg ctcatcgatt ggccgctgga
     1621 ttaactggtt cacgctgtct ggcactgaag gcctgaagtc attgccgtca ggacgtggga
     1681 gacgttgtcc gttcgcacat gcttgcgcat tattgcttga gcttgttgag cattctcccg
     1741 gcgatttcgg ttatcagcgt tccctctgga gttgcgaact gctggcgata aaaatccgtg
     1801 aggtcatcgg ttgtccgcta catgctcaac catccggcga tggttacctg ctgccggact
     1861 ggtatggcgc agattagtgc caacacttcg tatccgggac acgaataagg aagaaaaaat
     1921 ggcagcgatt aatgtggcgt tagtcagttg tagcacagaa ccctcggcgt tttactaaga
     1981 tgaagaggat atccacctca atcccaaaat tggcgctgac tggcagttac gtgggcagaa
     2041 atagcaaagg ttcatcatta tttatcagta tgttaaaaca tctgaaggcg acatacctgc
     2101 gggcaaaaac gataacactc attgtcgata actacatcat ccacataagc cgtgaaacgt
     2161 tgcgctggct gaaagcaaac ccaaagttca agggcattta tcagccagtt tactagccgt
     2221 gggtcaatca tgttgctgac tttagtcagt ttcgggcgca tggcggctga aacagataac
     2281 cacctgctat gagggggggg ctgattttga acggttgtgg caagcgtttc atgaaataat
     2341 aactcaaaat cacctgtgca gttcaatgtg gcagctgttg aacaaggttc gtcacattat
     2401 ggatgccgtc agctcatttc ccagtggaaa gcataggctg gctaaagtgt agcggtatta
     2461 ggagcagtta tttagcagaa gatataatga aaagctacca catcaggagt tgacgttcac
     2521 tacttttgat ctttggataa taaacatcga atggcaatca tatgaagaaa aaattgataa
     2581 ttatcggagc tggcgggttt gcaaaagctg taattgatag tttagatcat gcatgttatg
     2641 aaattcatgg tttcatcgat agttttaaat gcggagaaca tcaaggctat aaaataattt
     2701 cgagaacaat agatagttta gatgaacctc ataaatattg ctactttatt gcgattggcg
     2761 aaccctatta tagggctatc tggttggaaa ccattaatgc catgaaactt gaaactataa
     2821 atgttattga cagaacggca ataatttcta aaggcgcaat tcttgggtca tgcatctatg
     2881 ttggtaagat ggcaatagta aattgtgatt cgcatcttga ggatggtgtt gtcattaata
     2941 caagggctct cgttgaacat ggtaattata tatcatattg ttccaatata tcgactaacg
     3001 tcgtcttgaa tggtgatgtt tttgttggta gaaaatgttt tataggaagt tgtacagtcg
     3061 ttaatggaca actgaaaata ggcgattcgt ccatagtcgg ctctggttca gttgttattc
     3121 gaaatgttga tgaaaacgtt gtagtcgctg gttctccaac tagacttatt aggaagaggt
     3181 aaaagaatgt catctgtata tatagttgcg gaaattgggt gtaatcataa tggagattat
     3241 aatttagcta aattgatgat cgatgaggct tatagtgctg gtgtagatgc tgttaaattt
     3301 cagacattta aagctgatag cttaatatca aaatatgcac ctaaagcaga gtatcaaatt
     3361 aaaacgacag gaaatagtga gtcacagttg gaaatgactc ggaagttaga gctttcctgt
     3421 gaggaatata tcaagttaga agagtattct aaaagtttag gtttggatgt tttttcaact
     3481 ccttttgatt tggaatctat agaattttta tacacaagaa aacaaactac atggaaaata
     3541 ccatctggtg agttgcttaa tcttccatat ttagaaaaaa ttgcgttatt gccaggtgat
     3601 aaaaaaatta ttctatcgac aggtatggca actctcgccg aaataaagca agcattgaag
     3661 atatttactg ataatggagt taaagccgag gatattacta tcttacattg taatacagag
     3721 tatcctacac cttatgaaga tgttaatctt aatgttcttg ctggattaaa aaaaatcttc
     3781 caagaatata acattggttt ttctgatcat tctcctggat actttgccgg tatagcggct
     3841 gtaccttatg gaataacgtt tattgaaaaa cattttactt taaataaaga ttttgaaggt
     3901 cctgatcata aagcatcgat tactcctgat gagctgaggt tattgtgtaa tggcataaga
     3961 gcgattgaaa gagcattagg tagttttgat aaaagagtta ctaactctga gaggaaaaat
     4021 aaactagtgg caagaaaatc tattgttgct aagcgtgata taaaaaaagg ggaaattttt
     4081 actaccgata acattactac aaaacgaccc ggaaatggaa ttagtcctat gtattggtat
     4141 gaggttttag gtaaaaaagc ggagcaggat tttttagagg atcaattaat aaaagtatct
     4201 ggttttgaag aacaagagat ttaaatatga ggaaaaagat tgcaattatc ccagctcgtt
     4261 ctgggtcaaa aggattaccc aataaaaata ttttaatgct ttttgataag ccgttaattg
     4321 catatacaat tgaagctgct atcaaaagtg aattatttga gaaagttatt gtttcgacag
     4381 actcttgtga atataaggat attgctttac gccatggtgc tgaagttatt atgagggaca
     4441 aaagtctagc ttctgataca gcaacatcat acatggttgt aaaagatgtg ttagagaaaa
     4501 atattagctg tgattatttt gttttattac agccaacttc gccatttagg aattataaac
     4561 atataaggga agctgttgaa aagtttgaaa atacagatga cattgatttt ttggtttcaa
     4621 tggttaaaac aaataaaagt gcagatttaa ttaatcagtt ggctcccaat gataatttaa
     4681 aatatttcaa tgcggacttt gctaattata gaaggcaaaa ccaagagaac tattgtccta
     4741 atggtgccat atttataggt aatgtcgatg catatcttag acagaaacat ttttttggtg
     4801 aaagaagtat tgcttatgtg atggatagaa ttgattcatt agatattgat gacaaacttg
     4861 attttgagtt tgcgatatct ctaatgctta aaaaaaatag gatgaaagac cttcaaaagg
     4921 aaatcttgaa aagaataaag gaaaaggaat ttttttttga taaaataaag ccggtgacat
     4981 tagttggtca ttcaattatc gattattgga atgttactca tttaaatgga cgtgaagtga
     5041 ataatttagg tattgctggc atcaatagta aggagtatta tgatcttatc ataaaaaata
     5101 acattattaa atttggtgat acagtcatac ttatgcttgg tacaaatgat attgttgttg
     5161 atgactggag tgttgacttt acaattcttt ggattgaaaa aatagttaaa catataaaaa
     5221 aaataaatca caatgctagt atatatattt tgtcgatccc gccagtgttg ggacgtattg
     5281 atagaaacaa tgaaacaata agaaaattaa actccgaatt gtcaaagttt tttgaaaatt
     5341 atcaaaatgt tatttttata actctttcaa aagtttttta tgatcaatat ggaaatctaa
     5401 aggaaaactt tacatatgat ggactgcact ttagtcagtt agcttataat caattgacga
     5461 aagagataga ggaaaaaata aaatgaaaaa aatattatat ataacaggct caagagcaga
     5521 gtatgggata atgaagaggc tgttaagaaa acttaaggat aacgaacata ttgaactttc
     5581 aattattgca accggaatgc attgtgatgc gaagtatggc tatacatatc agaatattat
     5641 tgacgatggt ttcgaagtta aagagttatt tgatataaat atagagtcat caacaaacag
     5701 tggtatcatt tctacaatga gtcgagctca aaatttgttt ggtgcttatt ttgaggaata
     5761 taaatatgat gcaataataa tacttggcga tagatatgaa atgctttctg tagctatagc
     5821 ggcatctata catggtattc caataatcca tttacatgga ggagaacaga cactaggcaa
     5881 ttatgatgaa ttcattcgcc attgtataac aaaaatgagt cacttgcatt tagttgcaac
     5941 agagaaatat agggaaagag taatacaact aggcgagttg ccgtcttggg tcataaatat
     6001 aggtgctatg ggagccgaga atacagtatc tttgtcaata ccagaacata aagagctaca
     6061 atcaaaatat gaaaattcat taagcaatta tttcatgatt ctttttcacc ccgaaacctt
     6121 aactaaaaat aaatctgttc atgatcaaat aaaagaatta attaaagcat tggatattgc
     6181 acatcaaaaa tatcattgta attttatatt tatcggtact aacgcggata caaattcaga
     6241 tattattact aaggaaattc tcacgttttg tgagtcatat aactttcctt atttactatc
     6301 attaccaact caagattact taggattatg taaatattcg ttaggactta ttgggaattc
     6361 atcttctgga ctaatagaga tcccatctct tggagtgcct actattaata ttggttctag
     6421 acaggctgga cgagaacgag gaaagtccgt gattgatgtg gaatgttgct atacagatat
     6481 tcttgagaag atagatgacg ttatttcaaa tcgaatagat agaacttatt ataatcctta
     6541 ttataaagaa aatagtgccg agatagctat gtctagtata attagtttct tagattggat
     6601 tagtgataac aaaaatcgca ttaaaaagtt ttatgatatt cctaaagtca ctactctaat
     6661 taattcataa taaccaggca gtcttagtct atgtaacact agttcacatt actaaaagta
     6721 acagttgtaa aagtagatat actacctatt tactctctaa tcttcactat ctctaacata
     6781 tcataaatat taagataatt atttgtggtg attaatatac acgattattg attctgatat
     6841 aaccgaaaat ctttgaaatt acagtccgag aacgaggtag ttaagatgac ataaacatat
     6901 aagtttctct tgaaccccat ctgcgggctg ttcgtatata tttggggagt cagagcgaat
     6961 atgactcact gtggataact atttgttaaa ggctggctgt tcatcatatg tatctggata
     7021 cgccagcatt agcgggatat taggcgatga tggggactta ctggtgctga atattagttt
     7081 cggaaaaact tgaatgtcaa aggcggaaac tgtgctacag ataatgatat cttttgccag
     7141 gcttcttcct attttacgca cgcggagttt attgcttcct gaaaaaatat gggactactg
     7201 aatacgttgc gtgagcatta ctgaggcgga cctgtatgga gtgagctgta tattgcccca
     7261 ggaacaatta ctgatgctgc cttttggtgc taattaatac ttactatgtg ttcaataatt
     7321 gagttattcc tttatctaaa aatttttatc gtcgcttata tctgatcaaa taccggttat
     7381 ataaataagg aggacgaaat gaaatttaaa taatcagtgg aacatatccg acagttaaat
     7441 atttgtgccg tcacatttct ggtgcagaag ttggcagata tagaagattt tggttataca
     7501 catccaagca atatggtagt ttgaaaacgg tattcctacg cgctgtacca ttgccagagt
     7561 tctatcctat atctctgaga aaaatgtatg aacgatgacc agtcggaaag atactgctgc
     7621 agatcgctga gttattcctc agttcgtggc agaatgtttg gagcaactat gtgtggctga
     7681 ttttacttat ggtacgccct gagtttatat gtgcacgaaa gcccaaatga actaagagtc
     7741 tcttggaaca ttggacacaa tcatttttcg cgcggataac gactcggaag ttatctctct
     7801 ctcactgact ggcgagtaga aaaacatgga gtaaaactgg agtttatcct acagggtaag
     7861 ccaatgcata acacttgctt tgagcgcttt aaccggacat atcgtacgga aatactcgat
     7921 ttttatctgt tcagcacgct gaatgaagtg caggaaatca cggaaaaaag gttgtcagaa
     7981 tctaactcct catgaataac tgcacaatgt gactacggag aaatattgat aacacatgat
     8041 ttggccggaa gttcaaaaca gcatagatct aaagcaggta tctttacaga acagccccgc
     8101 gaatgtaggg atgttggtat aataatttaa gctgactgga aggtctgggc gagatgacac
     8161 tagtagacga taaaaagttt attgttattc atttaaaata ggagataaag atttgacggc
     8221 tttagtttag agataacacc tctctaggaa aattgaagaa gttctatttt gctgtaatgc
     8281 tttcttatgg atgtagtaat gaatgaagac agcagcaaat ttatatgtaa gtggaaaagt
     8341 tattcaaaag ggtatgtatg aaaagtaatg tttatatatg taaaacaaca tgggaaattt
     8401 tcgtttctat tttgttggct agcgatatat actacgacaa gaaaataaaa tctgtttttg
     8461 ttattgaagt ggctgaagag aacattaatt atctttctaa gttatctgat tttttctttg
     8521 tatcaaaggt tattcctgtt aggttttctt ctaataaata tgttaaggct ttatgttttc
     8581 attatagagt tagatattat ttgccaaaaa tgctcggcga gtatcttaaa agtgatgtaa
     8641 gggttaatct atttatagat cagggagtat ttggacaata ttttattaag aataataaag
     8701 atgtatatct atatgagcat gggaatggga attattgtgt aggcccatat cctaattata
     8761 aaatgataaa aaaatttttg ggtataactg aaggttatgg taggcacagt agagtaaaga
     8821 ggatatatct acagcatcca gaaaaagctc ctaaggatat tattcataag gtgacgggtt
     8881 tttctataaa gaatttatat aataaattgt tcttgaaaga acagcaaaac ttattgtctt
     8941 tttttggtat tcataaaatt aataaaacga gtgatgttat cattttaaca caaccaatca
     9001 gtgaggatgg tttttatagt gaaagagaaa agatcgaaat ctatagggag attattgata
     9061 gccgctttcc ttctgataaa tctcgcgtag ttataaaacc acatcctcgt gataaaacaa
     9121 attatggtaa gtacattcct gatgtaatgc ttttgccaag aagttttcct atggaaatat
     9181 taaacttcac gaatattagg tttattaaag caataacaat ctgttcaagc gctgtttata
     9241 attttgagta tgatatttct gtggattata taggaacatt aagatacccc aaattaatta
     9301 aagcattggg aagattgcct gcacagtatg tgcataagga taactataaa aacattcaat
     9361 attaacaggg tgttaatagg gagttaatat gataccttat gttatgtttt attgttcctc
     9421 tttagtttta gttgttctat ctttggtact gaaaaagtat gattggtttt ggttgttgtt
     9481 tcttctattt ttttcagctg tgtttgttgg tcttagggta gatgtcggtg ccgattatac
     9541 tgaatatgcc caaatttata atcaatctgg taatattaca aattttgaat tgggttttga
     9601 tataatattt aactatggga aaaggcttgg atatgattat gtatttgtgt ctttattttt
     9661 ctttttgcta actacattat tttttatcta ctcaatcaaa gagttgaatt ataagacact
     9721 tatttatttt tgttttttgc ttttcatgtt tgtcccttta acatcaacaa tccgacaagg
     9781 cttagctatt cctttttttg taatgtgcat tttgaactct gataggccca aagtctactt
     9841 tacaagtata gccttagggt gtctttttca ttactcaatt ttatttatgt tttttttctt
     9901 ttgggtcaga catattaagc agtcgtattg tagggctttt ttaattgttt tgcttttttc
     9961 attgttgagt atttttaata tcgttgagta tataattatg ttattaaatt atatgcctgg
    10021 tttaggggct ataagcacta aacttcttat gtatactcag cgatatcaag ttgagttttc
    10081 aatgaattct tttgtttata aattggctgg tttactattg attacaatgt ttatgccatt
    10141 tataaaagta aacgaaaaat tacgttgtag ttacaacttg tattttatta cagtatgtat
    10201 atttattttg ttgaaagaca atgctgtttt aacaaaccgt ttgacgtatg ctaccaatat
    10261 atcattggtg actttttttt cattgttatt atcgatgata cgtgggttct ataaaaaagc
    10321 tattgtttct attttgtttg tgttttattt ttcttttagt tattttaagt tcacagcgac
    10381 agatttaaga cactcagttg aatctgctta tatgccttat aaatccttgc ttataaactg
    10441 aataagacat taagttatga aattattgaa aagcagattt tcttattttg ccactgaatt
    10501 aataaataag tgcgtcccat tctttttatt accatatgtg tcttctgcgg tgggcgtaga
    10561 agtttatggt aaaatcgagt tatttaccac taattatata atattatctt tcctttttgc
    10621 cttgggggtt gaggggtgga tgaatgccca ttactttaaa atggctgaaa gtgattttat
    10681 tactgtactg tcatcatttt atctattaag ttttctgatt tttgttattt gtatggcaat
    10741 atctctacca tattcatatt attgttctgc gcttcttttt gttggttacg ttcaatcgct
    10801 tcttaatatt acaactttac atttgagact aaagggtaaa tatttaaatg ctggattttt
    10861 attattgtta tcatcaattg ttaatgctgt gatggtatat gcgagttttg attggtttgg
    10921 taagacttat gagtcgcgta ttaactcttt tttgttttct tccattataa tacttgtgtt
    10981 tattgtgttt ttaagtatac gtgataagaa tattaaaata aaaattgatt ttagcttttt
    11041 aaatagagac attttattgt tttgcctgcc tctgtgtttg actatgatta ttaattgggt
    11101 taaaggtaat attgataaat attttattgc taatctactt gatttgaagt ctcttgggat
    11161 ttatgcggtt gcttatcagt tggcttcagt tataaatgtt gtcgggatta ttttgaacag
    11221 gaccttacag gctgacctgc ttaaaagcat ggctgatggt ttttatcaaa caaaaaaaat
    11281 aatattcact tttttattta tattgtgtgt gttttttttt ctgtatatag tagctgtgta
    11341 ttgtggcttt agttatgttt tttctatcga ctactcctct tctcgcaata tagctttact
    11401 gttgatgatt ggttttttgt tattatcttt ttcctcattt ctaattaatt ttttattatt
    11461 ctacaggagg ccaaggttga tattgtttca aaccttttta attacgatga tacatgtgat
    11521 gctatctttt attttgatta aaaaatacgc gctgcttgga gttttactta cacagttcgt
    11581 tacttcgtta ttaacctttg tatccacttt attagtatgc cacatattaa cgaaatcatc
    11641 tcaaaaatga caggttctta aatcgagaga agaaggcatt taacataatc taaaattaaa
    11701 gttgcatatt atctctaata aacaatgtta aaataactga acatcatagc cggtatggca
    11761 aaaataggca tggttttgct ctcaataacg gaaattatgg gtatttcgtc tctattttcg
    11821 actgtttacg tgaaaaggtg gaagcagtta ttgctgacaa tccagcgcag aaatttatac
    11881 tgtgaaagat aatcgtttaa ttattgaaac tcctcgtcgc aacctgttaa tggtgaaagt
    11941 aggtgttggc acgatactgc attgattcac agaaaccata tctagataat gtcagcgcca
    12001 gtgatataag acgttaatag ggatgtggac aatttcctgg actctcagga gatgaaccat
    12061 gtttacttaa gacgaaaaaa ttcgcaccat tgagctttat ttcaattatg gtaacgtggg
    12121 ttgagctatc tttcgaaaag aaatctgcag ttttggatca gcagggggtg gcgcaaaaga
    12181 atccattcgt aatagacacc gttactcaga tgtgcaaaaa caggttgccg ttgaacatta
    12241 tcttaaccac gactgctgtc tggcatttac cggcagaatt ctgggttatc cctgtaccga
    12301 tgtgctcgct cgctgggtta atgaacgtta ccccgacaga cgacgtatat ttaccagtaa
    12361 gaccagtccg gatacgttct ttgaaccaga agtcaaacgt caagctgtca cggcactctg
    12421 tacatgacga gtatcagaca gtgaagttgc cagacgtgtt ggtgccagcc gtactgtatt
    12481 gtataaatgg taagatgaaa ttatcggtaa cagtgtttac caaatagcat aaacataacg
    12541 aaccttctct ggaggcagaa cgcgatacgt tgcgggagga tgtcgccgac tgaaacaaga
    12601 aatacgtcgc agaagataga gctgcatatt ctgaaaaagg cggaggaaat cataaaaaaa
    12661 gacccgggca tcagtatcag tcacctgaac aacagggaaa aaacaaagat cgctgatgcc
    12721 ctgagacaaa catatcccct gacagaatta ctgcatgttc tgggccttgc ccgtagcagt
    12781 tatttttatc accgggctgc actgaaagct ggtgataaat acgccacgat acgtacgatg
    12841 ctgacagata tctttaacag taattaccag tgttatagct atcgtcgcct gcatgcgatg
    12901 ctcaggcatg aggcggttta gctatcagaa aaggttgtac gcagacttat ggtggaggaa
    12961 cagcttgtcg tcagccgtaa ccgtcgtcgc tacagcacat attgcagaga aatcggaccg
    13021 gctgctgata attttatcgc cagagatttt aaggcggagc aagctaaaca taaatggctg
    13081 acagatatca cggagttcca gttcctcaca gggaaaatca ggctatcact ggtggtggac
    13141 ttcttcgatg gaaaagttgt gagcagttct ctcagtgcac gtcccgaggc tgaactggtc
    13201 aacaccatag tggataaagc tgtcgaaacg taaaatgcag gtgaacgacc ggtgatatac
    13261 agtgacaaag gaggcattat cgctggccag gctagctgga aagagtgaat gcagcaggtg
    13321 ttattccctc aatgtcccat aaagggtgtc cacacgacaa tgctgcgtgc gaaggctttt
    13381 tcggcagatt gaaaacgaaa atctattatg ggcgtaaata atcgggcatc aagcctgata
    13441 aattcaggca tcatgtggat acttacatca gatggtataa cgagcggcgt ataaaattat
    13501 tgctggtgca gtcagctcag aaatgtaccg tcaacaatgt ggcgggcata ataaagcagt
    13561 tcaggaaatc gtcctcatct aaaaaattga ttgaacatat ttctattaac acatcagccg
    13621 ggaaagtgga acataagttg tctaaaattc tactgttgac aattttcgcc gtcattttgg
    13681 gtgcagaagg ctgggaagat atagagtatt ttggggaaac acctcttgat tttttgaagc
    13741 aatatggtga ttttgaaaat ggtattactg ttcacgatac cattgccaga gtgtatcctg
    13801 tatcagtcct gcaaaatttc acgagtgctt tattaactgg atgcgtgact accattcttc
    13861 agatgataaa gacgtcatcg caattgatgg aaaaacactc cggtattctt atgacaagag
    13921 tcgccgcaag ggagcgattc atgttattag tgtgttctca acaatgcaca gtctggtcat
    13981 cggacaggtt aaaacggata agaaatccaa tgagattact gctatccctg ctcttcttaa
    14041 tatgctggtt attaaaggaa aaattatcac atcagatacg atgggttgcc agaaagatat
    14101 tgcagagaag atacaagaat agggagatga ttatttattc tttgcaaaca gaaatcaggg
    14161 ttggcctaat aaaaattttg aggggaaatt ttcactgaaa gaattaaatc atccagatca
    14221 tgacagttac gcaattagtg aaaagagtca cggtagagaa gaaatcaatc ttcagattgt
    14281 ttgcgaagtc cgtgatgaac ttattgattt tacgtttgaa tggaaaggac tgaaaaaatt
    14341 atgtgtagca gttttctttc ggtcaataag tgcaaaacaa aataaagagc cagaaatgac
    14401 ggtcagatat tatattagtt ctgctgattt aaccgcagag aagttcgcca tagcgctccg
    14461 aaatcactag ctcgtggaga ataagttgca ctggtgtctg gacgtggtaa tgaatgaaga
    14521 cgattgcaaa ataagaagag gaaacgcagc agaattattt tcagggatac ggcacatcgc
    14581 tattaatatt ttgacgaatg ataaggtatt caaggcaggg ttaagacgta agatgcgaaa
    14641 agcaaccatg gacagaaact acctagcgtc agtccttgcg gggaaagggc tttcgttatc
    14701 ttgccctgat tatatcccct ctctgaaaga tccacgtgtc gtgaccatcc aaagtgaagg
    14761 ttagtctgca agtaaaacta gggtatgata aagctaggat caatgagtaa gtttgtcgtg
    14821 cgctgtcgta tctgatttta aatatttttt acacctaaat tttttctcag ttacgtgctg
    14881 gtttttagga gtgcaactgg atcctgaacc acggtgaaat cgtaaagaaa taattttgat
    14941 ttttttatag agactattac ttagttcagt tttatttgat aaaattaatt aattgaaaag
    15001 tagtgagatg tttttgtgag cggggctatt ttttagatat ttgtttactg tgacaatgaa
    15061 ctgatttgac acagtgcatg ttgctattag ccattgcctg ctcctcacta gcccatttag
    15121 tatcgtgaat acaaatgaag tagtggggaa tccattaaca catatatgcc atttatcaag
    15181 ttgagttgct atatacataa aaaaatcact tttactataa gagcgctata aaatgaaaaa
    15241 aatcactttt gttattgctg atattacttt tgttggaggt attgaaagag taaatacatt
    15301 acttgcgaat aaatttttta gcgaaggata taatgtagaa atcatatccc tctacaatac
    15361 caataaaaag gttaattatg agcttaataa ttgcattaaa gttaattttg tgaaagataa
    15421 ttcctactca ggtgcaccag gaagttttgg gcggctaaag caacacatca gttcacagtt
    15481 tgcgttattt aaggccatta aaaattcaaa tgctgatttt tatatcgtta attcatttcc
    15541 aatggctttt ttgtcatttt tcaatgtctt ttcaaataaa aaatatgttg ttattgaaca
    15601 tgtgcactat gactattacg gggtagccat caagtttttt agaaacttat tatataagtt
    15661 ttattacaaa gtggtttgtg ttaatgaaag tgacttgagt aaattttcca aacatttaaa
    15721 caatgctgtt aaaatcaata acccgctgag tttttcatct tcagaaataa cctgtttgag
    15781 cgaaaagaaa attatagcgg taggccggct tgagtatcaa aagggatttg atatattaat
    15841 agatattttt gcgagagtta ataaggaaca tcctggctgg gaacttcata tatatggcgt
    15901 cggaacttgt gaagagtttc ttagagataa aataaatcag tataaactaa ataatataaa
    15961 gttaatgggc tgtgttgata acatacagtt atattatcct aaatattctg ttttcgtatt
    16021 ttcatcaagg ttcgaaggct tcggaatggt tttattagaa gctatggagt gtggtttgcc
    16081 atgtatttca tttgattgtc ctactggtcc atctgaaata ctagggaatg gacagtatgg
    16141 catcttggtt gagaatggga atgttactaa atttagtgcg gaattagctg atctaatgtc
    16201 cgataaagaa aagaaaatta aactttcaaa tatatcaaaa actcgtgcaa aagaatttca
    16261 tatagacaag atttttagtg aatggagaaa attaattaat tagtacgcat agtatgtaac
    16321 tgaaaatatc gtacaaatga tttttatcag aagattacat gctcaattaa tataattaag
    16381 taagttacaa atctctgaca ggagtaaaca atgtcaaagc aacagatcgg cgtagttggt
    16441 atggcagtga tg
//