LOCUS       AB811606               16589 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli DNA, O-antigen biosynthetic locus, strain: E14a.
ACCESSION   AB811606
VERSION     AB811606.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 16589)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..16589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E14a"
                     /db_xref="taxon:562"
                     /note="serogroup:O22"
     gene            <1..896
                     /gene="wcaM"
     CDS             <1..896
                     /gene="wcaM"
                     /codon_start=3
                     /transl_table=11
                     /protein_id="BAQ00730.1"
                     /translation="LRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHL
                     IERINCTNGKINWGIGIGLAGSTYDNNYPENQAVKNFVVANITGSDCRQLIHVENGKH
                     FVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKY
                     LSIPQNFRVNDIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHL
                     FMRNINVMQESSVGPALSMNFDMRKDVRGVFMAKRKTLLSLTNIHAVNEKGQSSVDID
                     RINHHIVNVEKINFRLPERRE"
     gene            1054..2049
                     /gene="gne"
     CDS             1054..2049
                     /gene="gne"
                     /note="O022_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4-epimerase"
                     /protein_id="BAQ00731.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALVGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            2292..3185
                     /gene="galF"
     CDS             2292..3185
                     /gene="galF"
                     /note="O022_02"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ00732.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     CDS             3504..4595
                     /note="O022_03"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ00733.1"
                     /translation="MKVLFLITRGDELGGAQTHVKDVILGLINKYNIECYLACGTKGI
                     FTDIMEENNINVIHIDSMKREICFGDIIALKKLNDIIKDINPDIISCHSSKAGVLGRL
                     ASLGTRTKKVFTAHGWAFTEGISPKKAAIYKKIELLLSYITDATINVSYYDKKLALKA
                     GLKSQHYVIHNCIPDVHYEKNNGIANKTVLEFIMVARFCAQKDHETLLKAFSNIDKEK
                     WRLTLIGGGDSKSIKELAKKLNIDNNINFVGQTKNVVDFLNHSDVFLLISNWEGFPIS
                     ILEAMRSSLPIIATDVGGVSEAVKHGYNGFLINKGNVAELEETIIKIIESYSSLKILS
                     QNSRKTYCERFKVDKMIDLYASIFDEVLK"
     CDS             4592..5380
                     /note="O022_04"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ00734.1"
                     /translation="MKKTIILIPVYNDKGDLLKTLNSINEDDDNFEILIIDDGSVIPL
                     TLPREKYKFKINIFRLSENMGITKALNTGLDIISNRKDIDYIARLDAGDIQQPNRITI
                     QKKTFESDSELVMLGTNVKFYDTNHNYLFDTKLPLTDIQIKKEKYIRSCFIHPSVMFT
                     KRIIDQGMRYSTKFPAAEDYEFFLRIILNYKVANLDKSYVYCFDRATGISIRKRNIQI
                     NSVLKCLFKHRDYTSTYWYFGVFKVLMQKIFPRKVFNLIKSKQK"
     CDS             5377..6246
                     /note="O022_05"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ00735.1"
                     /translation="MIKTTVIIPYKNNPDALKRALVSVSSQTVPVSIIVIDDNSEKWC
                     SAEKIVQEQNKFSDITIITHKNNQGGGAARNSGIIKAKTQYIAFLDCDDYWDEDKIEK
                     QEVLFENIKNEKAIIFSSIKVVNENLDVIKLYDNDNIIVNNFASYVFKDSGLIQTSSI
                     FLDTNFAKKNLFDASLKRHQDYDFCLKLQKDGGIFYGCGGTFSYWVVPQDPLIALKKG
                     YDTNLSIDFYQKYKTLMGEKTSCYFLVKTPMWFSLKQRKLFSFLLNLSRKCGVKTTLR
                     VLMTAFELAFKRV"
     gene            6247..7482
                     /gene="wzy"
     CDS             6247..7482
                     /gene="wzy"
                     /note="O022_06"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="BAQ00736.1"
                     /translation="MNNNIIDTAGRLKLIAFNFMIFVFLVRVPGFPFIILSIISITIL
                     SLFLIKVKVELNNKLIPLYCFVIYMILRCVIGFVFNGGYEERYFMVLLLMLFVLIVFT
                     VELKDVRLEQCYNGISAAVIVTLLIVLYEIISGNHMSTSRYSDINNEAFLWSVSKPTA
                     FYYNENDMLSFVVCFLPFTFVLKNTMLRVAIFLLAAASAIYIGSKAAIITILIYGLFT
                     KIISKKAILLLVVTGVVLVNLFATSLIENTSSFISGSSERLLGFWDTILSTRGGDEST
                     SERLSIYKANLDWLGNHPLSATFGNGRFGDYESELMKVYGLRMGEFHNLHLEMITIFG
                     LLFYLLFFSYYFYLWICLFLKKNKNTACYPLLISSAMYPLIISFGPSSSLKYPFLFIL
                     CLLMILGFRNDEKHAKKNI"
     CDS             7451..8035
                     /note="O022_07"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acetyltransferase"
                     /protein_id="BAQ00737.1"
                     /translation="MMKSTLKKIYRTLLLIQMSPFLVWALLKNKLPYSNYKNITIAMS
                     KFTGEKGVILRQYFYKLTLDSCGHNLRVHYGAFFVYPNVKVGNNCTVEEYSIVSLCDL
                     GDDVIIAARVSIMSGGNHHEIDKLDIKFVDSLLPLKKVKIGSNVWIGTHAIVMNDIPD
                     GMVVGAGSVVTKVFQPDSIIAGIPAKVMRKRGKS"
     CDS             8032..9192
                     /note="O022_08"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ00738.1"
                     /translation="MKITVIPSWYPNPKMRTQGLFFRDQARALSKEHEVDVLYCHRGK
                     RFYSNLYTDDGIKTFYWGYRLRIGSILGMISRIFIYCMMFILYEKKRKVDVIHCHSVA
                     FNQDGSAGIAGVILGKLFSIPVVITEHATVFSNKNYGRFEKRLMRWALSNADMIICVS
                     EGLRDILEEMTLRQDILIIPNTIDFKTFVNNDIVVHKIKKENDKIKICSVGYLMEKKG
                     FDRLINVINKLIKDFGYDIELSIVGAGPQYDSLSILIRQYDLQDSIFLLGELDKKQIS
                     ELMLSSDLFILLSRVETFGVVIIEGLATGLYCVATKCGGPEYIIKDKKLGHLLKNTND
                     VEYLSSEIDNLLQNKTYDKDQIYRMNYARQEYGFERFLELFNDLVLKKYENK"
     gene            9179..10396
                     /gene="wzx"
     CDS             9179..10396
                     /gene="wzx"
                     /note="O022_09"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="BAQ00739.1"
                     /translation="MKINDLLKKYTLFSLSPLLSAFIQLASIFLLGHSLSGPELGIAG
                     IYNTIVFILVFISDGGSSSYFIYRKFLPKYDVNLINIFNVAISAFAIIIIGYYVPEKS
                     DLLGLIVVSLTVTLPLYNYARLLVEKKYNIIAIVELKSKLLFFITLYIAINEFDYQGS
                     AVVMSWAVSYFLRYCLFWWFSKKIELYKNDNLPQNKPAAILKSWWMYIHNQILSQVLN
                     YFTITFDIFIISHFGGLEKVGVYSLCKDATLKISAVLSPVISKLLISHVVNIEENKIS
                     PMYKKVYKATIIISISVFGIWALCGSSIIHFIRPQLDQGILTFIIGWSICGILRMMIN
                     PIVSIFQATGKTRNELYVNITSAVSFIFFMGMLSIYFDNIADAVILALICMYSISFVY
                     ATVLLRKYFSKIY"
     CDS             10407..11423
                     /note="O022_10"
                     /codon_start=1
                     /transl_table=11
                     /product="putative UDP-glucose 4-epimerase"
                     /protein_id="BAQ00740.1"
                     /translation="MKILITGGTGYIGSHTILMLLNEGYDIIVIDNFSNSSPKVIERI
                     EFLSQKKVCFHEGDIRDERFLKSVFKEHVITTVIHFASLKSVSESISMPIEYYDNNIS
                     GTLTLLKVMNYFGVNNFIFSSSATVYGAPSRIPLDESCDTGDTTNPYGTSKYFLEQIL
                     IDTQRANPALNIMLLRYFNPVGAHSSGFIGEDPNGIPNNLFPYITQVAIGRLPRLSIF
                     GDDYPTEDGTGVRDYIHVMDLASGHVAALNRQHVLTGLRCYNLGTGKGYSVKEIVETF
                     QKITGKKIPYIVSARRPGDIAECWSSPELAQKELHWEAKRSLEDMVIDAWNWQNKNPN
                     GYLE"
     gene            11542..12948
                     /gene="gnd"
     CDS             11542..12948
                     /gene="gnd"
                     /note="O022_11"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ00741.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKGEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            13197..14363
                     /gene="ugd"
     CDS             13197..14363
                     /gene="ugd"
                     /note="O022_12"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ00742.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            14453..15487
                     /gene="wzz"
     CDS             14453..15487
                     /gene="wzz"
                     /note="O022_13"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ00743.1"
                     /translation="MAIIAKLWRLKIILSVRVMMRVENNNVSGQNHDPEQIDLIDLLV
                     QLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGS
                     AAPKASEIQSMLIGRFSTTFSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETP
                     EGAQKQLAKYIQQVDDQVNEELEQDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQ
                     IQEALQYANQAQVTKPQIQQTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQT
                     RQNLLDIDNLDVDKLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLG
                     RNALRNYNAK"
     gene            complement(15673..16284)
                     /gene="hisI"
     CDS             complement(15673..16284)
                     /gene="hisI"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ00744.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLHQLEQLLAERKSAAPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
ORIGIN      
        1 tcttacgcca gggatttcat aaccagataa tcggtgccaa catcaccaac tgtaagttca
       61 gcgacttaca gggcgacgct attgaatgga acgtggcgat taacgacagt gatattttga
      121 tatccgacca tctcattgag cgcattaact gtaccaacgg caaaatcaac tggggcatcg
      181 gcataggcct tgcgggaagt acctacgata ataattatcc ggaaaaccag gcagtgaaaa
      241 actttgtcgt ggcgaatatc acgggatcgg attgtcggca actgatccat gttgaaaatg
      301 gtaaacattt tgttatccgt aatatcaaag ctcgtaatat cacgccggat ttcagtaaga
      361 aagcaggtat tgataacgcc acagttgcta tttatggttg tgacaacttt gtgattgata
      421 atattgaaat gattaatagt gccgggatgt taatcggcta tggggtaatt aaaggcaaat
      481 atctctcgat accgcagaat ttccgagtga atgatattca actggataat actcatcttg
      541 cttataaatt gcgcggcatt caaatctccg ccgggaatgc tgtctccttt gtggcgctga
      601 ctaacattga gatgaagcgt gcgtcgctgg agttacacaa caaaccgcaa catcttttta
      661 tgcgcaatat caatgtgatg caggaatcct cagttggacc cgcattgagc atgaactttg
      721 acatgcgcaa agacgttcga ggcgttttta tggcaaagcg taaaacgctg ctttcactca
      781 cgaatattca tgcagtgaat gaaaaagggc aaagctccgt cgatatcgac aggattaatc
      841 accatattgt taatgtggag aagattaact ttagattgcc ggaacggagg gagtagattt
      901 gcgaccattc ataaacttac tggatataat gttctaaaag attataatcc tcacactaac
      961 tgttaaagct gggactgggc agcattgact actcagggaa ggtactcatc tttatcttgt
     1021 gtttcttacg taacaaccag actaaagtga tttatgaacg ataacgtttt gctcatagga
     1081 gcttccggat tcgtaggaac ccgactactt gaaacggcaa ttgctgactt taatatcaag
     1141 aacctggaca aacagcaaag ccacttttat ccagaaatca cacagattgg cgatgttcgc
     1201 gatcaacagg cactcgacca ggcgttagtc ggttttgaca ctgttgtgct gctggcagcg
     1261 gaacaccgcg atgacgtcag ccctacttct ctctattatg atgtcaacgt tcagggcact
     1321 cgcaatgtgc tggcggccat ggaaaaaaat ggcgttaaaa atattatctt taccagttca
     1381 gttgctgttt atggtttgaa caaacacaac cctgacgaaa accatccaca cgatcctttc
     1441 aaccactacg gcaaaagtaa gtggcaggca gaggaagtac tgcgtgaatg gtataacaaa
     1501 gcaccaacgg aacgttcatt aaccatcatc cgtcctaccg ttatcttcgg tgaacgcaac
     1561 cgcggtaacg tctataactt gctgaaacag atcgctggcg gcaagtttat gatggtgggc
     1621 gcagggacta actataagtc tatggcttat gttggaaaca ttgttgagtt tatcaagtac
     1681 aaactgaaga atgttgctgc aggttatgag gtttataact acgttgataa gccagacctg
     1741 aacatgaacc agttggttgc tgaagttgaa caaagcctga acaaaaagat cccttctatg
     1801 cacttgcctt acccactagg aatgctgggt ggatattgct ttgatatcct gagcaaaatt
     1861 acgggcaaaa aatacgctgt cagctctgtg cgcgtgaaaa aattctgcgc aacaacacag
     1921 tttgacgcaa cgaaagtgca ttcttcaggt tttgtggcac cgtatacgct gtcgcaaggt
     1981 ctggatcgaa ctctgcagta tgaattcgtc catgccaaaa aagacgacat aacgtttgtt
     2041 tctgagtaat gcagacgagt cagagaaaaa agaaccaggt tggactacga ttattcggaa
     2101 agaggtactg attaaattat cagtatgtgg atttccgagt aatccgagta acccagatgc
     2161 tgatggtgtg tagtgttatc gtaaggttta atcaaagtta tgcggagcta agctttggta
     2221 tatagccata acgtttggct aacctatggc aataatttac gcactatcag ccacttagag
     2281 gtaggaaaag aatgacgaat ttaaaagcag ttataccggt agcaggtctt gggatgcata
     2341 tgttgcctgc cactaaggcg atacccaaag agatgctacc gatcgtcgac aagccaatga
     2401 ttcagtacat tgttgacgag attgtggctg cagggatcaa agaaatcctc ctggtgactc
     2461 atgcgtccaa gaacgcggtc gaaaaccact tcgacacctc ttatgaatta gaatctctcc
     2521 ttgaactgcg cgtgaagcgt caactgctgg cggaagtaca gtccatctgt ccgccgggcg
     2581 tgaccattat gaacgtgcgt cagggcgaac ctttaggttt aggccactct attttgtgtg
     2641 cacgacccgc cattggtgac aatccatttg tcgtggtact gccggacgtt gttatcgatg
     2701 atgccagcgc cgacccgctg cgttacaacc ttgccgctat gattgcgcgc ttcaacgaaa
     2761 cgggacgcag ccaggtgctg gcaaaacgta tgccgggtga cctctccgaa tactccgtca
     2821 tccagaccaa agagccgctg gaccgtgagg gtaaagtcag ccgcattgtt gaatttatcg
     2881 aaaaaccgga tcagccgcag acgctggact cagacatcat ggccgtaggt cgctatgtgc
     2941 tttctgccga tatttggccg gaactggaac gtactcagcc tggtgcatgg ggacgtattc
     3001 agctgactga tgctattgcc gagctggcga aaaaacaatc cgttgatgca atgctgatga
     3061 ccggcgacag ttacgactgc ggcaaaaaaa tgggctatat gcaggcgttt gtgaagtatg
     3121 gcctacgcaa cctgaaagaa ggggcgaagt tccgtaaagg tattgagaag ctgttaagcg
     3181 aataatgaaa atctgaccgg atgtaacggt tgataagaaa attataacgg cagtgaagat
     3241 tcgcagcaaa agtaatttgt tgcgaatatt cctgccgttg ttttatataa accatcagaa
     3301 taacaacgag ttagcagtag ggttttagtc aaagttttcc aggattttcc ttgtttccag
     3361 agcggattgg taagacaatt agtaccttat tatttatata agaaaatgaa tgggtaacac
     3421 caattgagtc atagacatgt ctgcagtacg ctggtagctg ttaagccagg ggcggtagca
     3481 ttcttatcta tatggaataa aagatgaaag tattattttt aattacaaga ggagatgagt
     3541 taggtggcgc acagactcat gtcaaagatg ttatattagg attaataaat aagtacaaca
     3601 tagaatgtta tctcgcctgt ggtacaaaag gcatattcac agacattatg gaagaaaata
     3661 atattaatgt aatacatatt gattcgatga aacgggaaat atgctttggt gatatcattg
     3721 cactaaagaa attaaatgat attattaaag atattaatcc tgatattatt agttgtcatt
     3781 catcgaaggc aggtgtatta ggtcgtttgg catctttggg aacccgaacc aaaaaagttt
     3841 tcactgcaca tggttgggct tttacggaag gaatatctcc taagaaagct gcaatatata
     3901 aaaaaattga gttactttta tcttacataa cagatgcaac aattaacgtt tcatattatg
     3961 acaagaaatt agctcttaag gcagggttga aatcgcagca ctatgtgata cataattgta
     4021 ttcctgatgt ccattatgaa aaaaacaatg gtattgccaa taaaacagtt cttgaattta
     4081 ttatggttgc acgtttttgt gcgcaaaaag atcatgaaac tttacttaaa gccttttcaa
     4141 atatagataa agaaaagtgg cgattgactt tgattggtgg tggagatagt aagtcaatta
     4201 aggagttagc gaaaaaactt aatatagata acaatattaa ttttgtaggt caaacaaaaa
     4261 atgttgtcga ctttttgaac cattctgatg tttttcttct tatatctaac tgggaaggct
     4321 tccctatttc tatactagaa gcaatgagaa gtagtttacc catcatagcc actgacgtgg
     4381 gaggagttag cgaagctgtc aagcatggtt acaatggctt tttgattaat aaaggaaatg
     4441 tagctgaact cgaagaaaca ataattaaaa taattgaatc ttattcatca ttaaaaatac
     4501 ttagtcaaaa ttcaagaaaa acatattgtg aaagattcaa agttgacaag atgatagatt
     4561 tatatgcatc aatattcgat gaggtattaa aatgaaaaaa acgattatct taatacctgt
     4621 ctataatgat aaaggtgatt tactgaaaac attaaatagc atcaatgaag atgatgataa
     4681 tttcgaaata ttaattatcg atgacggaag tgtcatacca ttaacattac ctcgcgaaaa
     4741 atataaattt aaaattaata ttttcaggct tagtgagaat atggggataa caaaagcgct
     4801 aaatacaggg ttagacataa taagcaatag aaaagatata gattatatag cgcggttaga
     4861 tgcgggagat atacagcaac caaatcgaat aacaattcag aagaaaacat ttgaatccga
     4921 tagtgaatta gtgatgcttg ggacgaatgt taaattttac gacacaaatc acaattattt
     4981 atttgatact aaattgcctt tgacagatat acagataaaa aaagaaaaat atataagatc
     5041 ttgttttata catccttcgg taatgtttac taaaaggatt attgatcaag gaatgcgata
     5101 ttcgacaaaa tttcctgcag cagaagatta tgaatttttt cttagaatta tattaaatta
     5161 taaagtggct aatttagata agtcatatgt gtattgcttt gatcgggcta ccgggatcag
     5221 tatcagaaag cgaaatatcc aaatcaatag tgtcttaaaa tgcttgttta agcatcgaga
     5281 ttatacatca acatattggt atttcggtgt tttcaaagtg ttaatgcaaa aaattttccc
     5341 gagaaaagtg tttaatttaa ttaagtcgaa acaaaaatga ttaagacaac agtaataatt
     5401 ccctataaaa ataatccaga tgctctcaaa cgagcactgg ttagcgtgag tagccaaact
     5461 gtacctgtta gtattattgt catcgatgat aattcagaaa aatggtgttc ggcggagaaa
     5521 atagttcaag aacaaaataa attctccgat attacgataa tcacgcataa aaacaatcaa
     5581 ggggggggcg ctgctaggaa tagtggcatt atcaaagcaa aaacacaata tatcgctttt
     5641 ttggattgtg atgattactg ggatgaagat aaaattgaaa agcaagaagt actattcgaa
     5701 aacataaaaa atgaaaaagc aataattttt tcatcaataa aagtagttaa tgaaaattta
     5761 gatgtgatta aattgtatga taatgacaat attattgtaa ataattttgc cagctatgta
     5821 tttaaagata gtgggttaat acagacatca tcaatttttt tggatacaaa ttttgctaaa
     5881 aaaaacttat ttgatgcaag tttaaaaaga catcaggact atgatttttg tttaaagcta
     5941 caaaaagatg gtgggatttt ttatgggtgt gggggaactt ttagttattg ggtcgtgcct
     6001 caagatccat tgattgcact aaaaaaagga tacgatacaa atttaagtat tgatttttat
     6061 caaaaatata aaacgttaat gggggaaaaa actagttgct attttctagt aaaaacgcct
     6121 atgtggttca gtttgaaaca acgtaagctg ttttctttct tactaaactt gtctagaaag
     6181 tgcggagtaa aaacaactct ccgagtttta atgactgcat ttgagctggc tttcaaaagg
     6241 gtctaaatga ataacaacat tatagatact gcaggtagat taaaattgat tgctttcaat
     6301 tttatgattt ttgttttttt ggttagagtt cctggatttc cctttataat tttatctatt
     6361 atcagtataa cgatactatc cttattttta ataaaagtta aggttgaatt aaataacaag
     6421 ctaattcctc tttattgttt tgtgatatat atgatccttc gttgcgttat aggttttgta
     6481 tttaatgggg gatatgaaga acgatatttt atggttttat tgttaatgct atttgttcta
     6541 attgtattta ctgtagaatt gaaagatgta agattagaac agtgttataa tgggatatca
     6601 gctgcagtaa ttgttacctt gctaattgtg ttatacgaaa taattagtgg taaccatatg
     6661 agtacatcac gttattcaga tataaacaat gaggcatttt tatggagtgt ttcaaaacca
     6721 acagccttct attataatga aaatgatatg ttgagctttg ttgtttgctt tttaccgttt
     6781 acttttgtat taaagaacac tatgttgcgt gtcgcaatat ttttactggc tgctgctagt
     6841 gctatatata taggttcgaa agctgcaata ataacgattc taatttatgg gttatttaca
     6901 aaaataatct ctaaaaaggc gatattactt ttagtggtaa ctggtgttgt tcttgtcaat
     6961 ttatttgcaa catcactaat tgaaaatacc agttccttta tatccggctc ttcagaaaga
     7021 ttacttggtt tttgggatac gatactttcg actcgtggtg gagatgaaag tacctcagaa
     7081 cgtctttcca tttacaaagc taatctggat tggcttggta accatccttt gtcagctaca
     7141 tttgggaacg gaaggtttgg tgattatgaa agcgaattga tgaaagttta tggtttaaga
     7201 atgggggaat ttcataattt acatcttgaa atgataacta tattcggttt gttattttat
     7261 ttgttatttt ttagctacta cttttattta tggatttgtt tatttcttaa aaagaacaaa
     7321 aatacagcct gttatccttt gttaatatca tcagcaatgt atccactaat aatctctttt
     7381 gggccaagta gttctttgaa gtatccattt ctttttattt tatgtttact aatgatatta
     7441 ggatttcgaa atgatgaaaa gcacgctaaa aaaaatatat agaacattgt tattaattca
     7501 aatgtctccg tttttagtgt gggcgttatt aaaaaataaa ttgccttact caaactataa
     7561 aaatataact attgctatgt ctaagttcac gggagaaaaa ggagttatac taaggcagta
     7621 tttttataaa ctcaccttag attcctgtgg gcataattta agagtgcatt atggggcttt
     7681 ttttgtttat ccaaacgtta aggttggtaa taattgcact gtagaggaat actctattgt
     7741 cagcttatgt gatctaggtg atgatgttat tatcgcagca agagtatcga ttatgagcgg
     7801 tgggaatcat cacgaaatag ataagttgga tataaagttt gttgactcac ttcttccttt
     7861 aaaaaaagtt aaaattggtt ccaatgtttg gattggaacc catgctattg tcatgaatga
     7921 tattccagat ggtatggtcg ttggtgctgg gagtgttgtt acaaaagtat ttcaacctga
     7981 ttcaattatt gcgggtattc ctgcaaaggt tatgagaaaa agagggaaat cgtgaaaatt
     8041 acagttattc catcttggta tccgaatcct aaaatgagaa cacaaggact tttttttaga
     8101 gaccaagcta gagcactttc aaaagagcat gaagttgatg ttttatattg ccatagagga
     8161 aaaagatttt attcaaattt atataccgat gatggcatta aaacttttta ctggggatat
     8221 agactgcgca taggaagcat tttaggaatg atatctagaa tcttcatata ttgcatgatg
     8281 tttatcctct atgaaaagaa aagaaaagta gatgttatac attgtcatag tgttgctttc
     8341 aatcaagatg gcagtgccgg tattgctggt gtgattttag gtaaattatt ttctattcct
     8401 gttgttataa cagaacatgc aacggttttt tcaaataaaa attatggtag gtttgagaaa
     8461 agattaatgc gatgggcttt atctaatgct gatatgataa tttgtgttag tgaaggcttg
     8521 cgtgacattt tagaagaaat gaccctaaga caggatatac taataatacc aaacacaatt
     8581 gattttaaaa cttttgtgaa taatgatatt gtcgtacata aaattaaaaa agaaaacgac
     8641 aaaatcaaaa tatgttcagt aggttatcta atggagaaaa agggctttga tcgacttatc
     8701 aatgtgataa ataaattgat taaagatttt ggttatgata tagagttgag tattgttggt
     8761 gcgggtccac aatatgattc gctatcaatt ctcattcgac aatatgactt acaagattca
     8821 atatttctac tgggggaact cgataaaaag caaatatctg aattgatgct ctcaagcgat
     8881 ttgtttattt tgctaagtcg agtagaaact tttggggttg ttattattga ggggttagca
     8941 accggattat attgcgttgc aactaaatgt ggcgggccag aatatataat taaagataaa
     9001 aaactaggtc atttactcaa aaatactaat gatgttgaat atctaagttc ggaaatagat
     9061 aatcttttac aaaataaaac ttatgacaag gatcagattt atagaatgaa ttatgctcgt
     9121 caagaatatg gattcgaacg tttcttggaa ttatttaatg atctggtttt gaaaaaatat
     9181 gaaaataaat gatttattga aaaaatacac acttttttca ttgtcgccac tactttccgc
     9241 gtttattcag ctagcttcta tttttctttt aggtcattca ttatcaggtc cggaactagg
     9301 tatagcagga atttataaca caatagtttt tattttagtt tttatttcgg atggcggtag
     9361 ttcaagttat tttatttatc gtaagttttt gccaaaatat gatgttaact tgatcaatat
     9421 ttttaatgtg gccatatctg cattcgctat aattatcata ggttattacg ttccagaaaa
     9481 atccgattta ttgggattaa ttgtagtatc actaacagtt acgcttccat tatataatta
     9541 tgctcgtttg ttagtagaaa agaaatacaa tattatcgct atagttgaac taaagtcaaa
     9601 attgttattt tttattacat tatacatagc aattaacgag tttgactatc aaggcagtgc
     9661 cgtagtaatg tcatgggctg tttcttattt tttaagatat tgtttatttt ggtggttttc
     9721 taaaaaaata gaattatata aaaatgataa tttaccccaa aataaaccag cggcgatttt
     9781 aaaaagttgg tggatgtaca ttcataatca aattttgtca caagtactaa attattttac
     9841 aattacattt gatattttta ttatatcgca tttcggggga cttgaaaaag taggtgtgta
     9901 cagtttgtgc aaagatgcca ctttaaaaat aagtgctgtt ttaagcccag taataagcaa
     9961 gttgttaatc tctcacgttg ttaatattga agagaataaa atatcgccta tgtataaaaa
    10021 agtatacaag gcaactatta taatctctat aagtgtattt ggcatttggg ctttatgcgg
    10081 aagtagtatt attcacttta ttagacccca actggatcaa ggtattttga cctttataat
    10141 cggatggtct atatgtggca ttcttagaat gatgattaat ccaattgttt ctatttttca
    10201 ggcgacagga aaaacaagaa atgaattata tgttaatatt acttcggcgg tctcctttat
    10261 tttctttatg ggaatgctat caatatattt tgataatata gccgatgctg ttattcttgc
    10321 tttaatatgt atgtattcta tttcgttcgt ttatgctact gttttattga gaaaatattt
    10381 ttctaaaatt tattaagggt gggtttatga aaatattaat aactggtggg actggttata
    10441 taggttccca tacaatttta atgttgctaa atgaaggtta tgatataatt gttatagata
    10501 atttttctaa ttcatcgcct aaagttatag aaagaattga gtttctttct caaaaaaaag
    10561 tatgctttca tgaaggggat ataagagatg aaagattttt gaaatctgtt tttaaggaac
    10621 atgtcattac gacggtaatt cattttgctt cacttaaatc tgtttctgaa tctatttcaa
    10681 tgcccattga atattatgat aataatattt ctggaactct gaccttgttg aaggtaatga
    10741 attattttgg tgttaataat tttatcttta gttcatctgc aacagtttat ggtgctcctt
    10801 ctagaattcc gcttgatgag tcgtgtgata ctggagatac tacaaatcca tatggaactt
    10861 caaaatattt tttggagcag atattgattg atacacaacg tgctaatcct gcattaaata
    10921 ttatgctgct gaggtatttt aatcctgtcg gagcccattc atcaggtttc atcggcgagg
    10981 acccgaatgg aattccaaat aatctatttc catatattac ccaagtggcc attggaagat
    11041 taccgaggtt gtcaatattt ggagatgatt atccaaccga ggatggtact ggagtaagag
    11101 attatataca tgttatggat ttagcatctg gtcatgttgc tgctctcaat agacagcatg
    11161 ttttaacggg attgcggtgt tacaatcttg gcacagggaa aggttattca gtcaaagaga
    11221 ttgttgaaac ttttcaaaag ataactggaa aaaaaatacc atatattgtt tctgcacgtc
    11281 gtcccggtga tatagcagaa tgttggtcct caccagagtt agctcaaaaa gaacttcatt
    11341 gggaagctaa gcgtagtctt gaggatatgg ttattgatgc ttggaactgg caaaataaga
    11401 atccaaatgg atatttagaa tgatgttata ttatttcttt taaaggcagt ttgctactat
    11461 caatattttg tttgaaatag tctaatatat gattccaaaa ccgcatacat cgcggtgacc
    11521 accccctgac aggagtaaac aatgtcaaag caacagatcg gcgtcgtcgg tatggcagtg
    11581 atggggcgca accttgcgct caacatcgaa agccgtggtt ataccgtctc tattttcaac
    11641 cgttcccgtg aaaagaccga agaagtgatt gccgaaaatc caggcaaaaa actggttcct
    11701 tactatacgg tgaaagagtt tgttgaatct ctggaaacgc ctcgtcgcat cctgttaatg
    11761 gtgaaagcag gtgcaggcac ggatgctgct attgattccc tcaagccata cctcgataaa
    11821 ggtgacatca tcattgatgg tggtaatacc ttcttccagg acaccattcg tcgtaaccgt
    11881 gagctttctg ccgaaggctt taacttcatt ggtaccggtg tctccggtgg tgaagaaggc
    11941 gcgctgaaag gtccttccat tatgcctggt gggcagaaag aagcctatga actggttgcg
    12001 ccgatcctga ccaaaatcgc cgcagtggct gaagacggtg agccatgtgt tacctatatc
    12061 ggtgccgatg gtgcaggcca ttatgtgaag atggttcaca acggtattga atacggagat
    12121 atgcaactga ttgctgaagc ctattctctg cttaaaggtg gcttgaacct cactaacgaa
    12181 gaactggcac agacctttac cgagtggaat aacggtgaac taagcagcta cctgatcgac
    12241 atcaccaaag atatcttcac caaaaaagat gaagacggta actacctggt tgatgtgatc
    12301 ctggatgaag cggctaacaa aggtaccggt aaatggacca gccagagcgc gctggatctc
    12361 ggagaaccgc tgtcgctgat taccgagtct gtgtttgcac gttatatctc ttctctgaaa
    12421 gagcagcgtg ttgccgcatc taaagttctc tctggcccgc aagcgcagcc agctggcgac
    12481 aaaggtgagt tcatcgaaaa agttcgtcgt gcgctgtatc tgggcaaaat cgtttcttac
    12541 gctcagggct tctctcagct acgtgcggcg tctgaagagt acaactggga tctgaattac
    12601 ggcgaaatcg cgaagatttt ccgtgctggc tgcatcatcc gtgcgcagtt cctgcagaaa
    12661 atcaccgatg catatgccga aaatccgcag atcgctaacc tgctgctggc tccgtacttc
    12721 aagcaaattg ccgatgacta tcagcaggcg ctgcgcgatg tcgtcgctta tgcagtacag
    12781 aacggtatcc cggttccgac cttcgccgct gcggttgcct attatgacag ctaccgtgcc
    12841 gctgttctgc ctgcgaacct gatccaggca cagcgtgact atttcggcgc gcatacttat
    12901 aagcgcattg ataaagaagg tgtgttccat accgaatggc tggattaatc tgatttaaat
    12961 caattaatca atgcaaggcc cggagaaacc ctccgggctt ttttattata caaagcggca
    13021 ggttatggcc tttttttata atttatggtt aaaaaagcga tataatacag cgccgcacag
    13081 caggatcgct gccttgacag ttcatctaca tcagcgttaa aaatcccgca gtagatgaag
    13141 ttgtggtgat ggattaatga ccactctaaa tgtttaaccg gaagaagtca gagctaatga
    13201 aaataacaat ttcaggaaca ggttatgttg gtctttcaaa tggtattctg attgcgcaaa
    13261 accacgaagt ggttgcactg gatatcgttc aggccaaagt ggacatgctt aacaagaagc
    13321 agtcaccgat tgttgataag gagattgaag agtatctggc gactaaagat ctcaatttcc
    13381 gcgctacgac agataagtat gacgcgtata aaaatgccga ttacgttatt attgccactc
    13441 ctaccgatta tgacccgaaa acaaattatt ttaatacctc aagtgtcgaa gcggtcatcc
    13501 gtgatgtgac agaaattaat cccaacgcgg taatggtgat taaatctaca attccggtag
    13561 gtttcaccaa atcaattaaa gaacgtctgg gtatcgataa ccttattttc tctccggaat
    13621 ttctccgtga aggtaaagct ctttacgata atctccatcc ttcacgtatt gtcattggcg
    13681 agcgttcaga acgcgcagaa cgttttgctg cgttgttaca ggaaggcgcg attaagcaaa
    13741 atatcccgac cctgtttacc gactccactg aagcagaagc gattaaactg tttgctaata
    13801 cctatctggc gatgcgcgta gcatacttta atgaactgga tagctatgca gaaagtttag
    13861 gtctgaatac tcgtcagatt atcgaaggtg tttgtctcga tccgcgtatt ggcaaccatt
    13921 acaacaatcc gtcgtttggc tatggtggtt attgtctacc gaaagatacc aagcagttac
    13981 tggcgaacta ccagtctgtg ccgaataacc tgatctcggc aattgtcgat gcaaaccgca
    14041 cgcgtaaaga ctttattgcc gatgccattt tgtcacgcaa gccgcaagtg gtgggtattt
    14101 atcgtctgat tatgaagagc ggttcagata acttccgcgc gtcttccatt caggggatta
    14161 tgaaacgtat taaggcgaaa ggcgttgaag tgatcatcta cgagccggtg atgaaagaag
    14221 actcattctt caactctcgt ctggaacgtg atctcgccac cttcaaacaa caagccgacg
    14281 tcattatttc caaccgtatg gcagaagagc ttaaggatgt ggcagataaa gtctacaccc
    14341 gcgatctctt tggcagcgac taacatcctg ttatcagggc gattttcgcc ctgattgtct
    14401 tatgttccct ttgtaataat tcattatttt tatcatttat cctatagcat tcatggcgat
    14461 tatcgctaaa ctatggcgac ttaaaattat tttgtccgtt agggtaatga tgagagtaga
    14521 aaataataat gtttctgggc aaaaccatga cccggaacag attgatttga ttgatttact
    14581 ggtccaacta tggcgtggca agatgactat catcatttct gtcattgtgg ctattgccct
    14641 ggctataggg tatctggccg ttgccaaaga aaagtggact tcaacagcta tcgttactca
    14701 acctgatgtg gggcaaatcg ctggttataa caatgccata aatgtgattt atggttcggc
    14761 agcgccgaaa gcgtctgaga tccagtcgat gttaattggt cgtttcagca cgacattttc
    14821 tgcattagca gaaacgctgg ataatcagga agaacctgaa aaactgacca ttgagcctac
    14881 cgttaaaaat cagtcattac ctttagcggt ttcttatgtc ggtgaaacac ctgaaggtgc
    14941 gcagaaacag ctggcgaagt atatccagca agttgacgac caggtaaatg aagaactgga
    15001 acaagacctg aaagacaaca tcgcgctgcg aatgaaaaac ttgcaggatt ctctgaagac
    15061 gcaggaagtg gtcgctcaag aacagaaaga tctgcgtatc cgtcagattc aggaagcgtt
    15121 gcagtatgcg aatcaggcgc aggtgacaaa gccgcagatt cagcagacac aggatgtaac
    15181 ccaggatact atgttccttt tggggagcga agcgctggag tcgatgatta agcacgaagc
    15241 gacccgtccg ttggtgttct catcaaacta ctatcagact cgtcaaaacc tgctggatat
    15301 cgacaatctt gacgttgata aacttgatat tcatgcttac cgctatgtaa tgaaaccgac
    15361 gttacctatt cgtcgcgata gcccgaaaaa ggcaattacc ttgattctgg cagtgctgct
    15421 gggcggcatg gttggcgcgg ggattgtgct tgggcgtaac gcgctgcgta attacaacgc
    15481 gaaataatat tattgtgcat ttaagagaaa cgggcagggt ggtgacacca tgcccgtttt
    15541 ttttgccgga tgcgatgctg gcgcatctta tccggcctac gtgtgttgag ataatgtgta
    15601 ggcacgataa gcttgcgcat cgggcaatgg ctccggatgc gacaacaaca tcgcatccgc
    15661 tgacctcgca actcactgat gccgtttacg caggttctca atcaccgttg ttaaatccag
    15721 atcctgatcc tgcaacaaca ccagcaggtg atacatcaaa tcagacgcct cgttggtcag
    15781 ttcaaagcgg tcatgtaccg ttgctgccag tgcggtttcc acgccttctt cgcccacttt
    15841 ctgcgcaatg cgtttagtgc cgctggcata cagtttggcg gtgtaggagg tttccggggc
    15901 ggcagattta cgttcggcga gcagttgttc cagttgatgc aggaacagcc actggtgagc
    15961 ggtatcgccg aagcagctgc tggtgccttt gtggcaggtt ggcccgatgg gattcgccag
    16021 caccagtaac gtgtcgttgt cgcagtccgg cgcaatattt actacgttga ggaaattacc
    16081 cgacgtttcg cctttggtcc acagtcgctg tttagtgcgc gagaagaagg tgactttgcc
    16141 gctttcgatg gttttgtcta aggcttccgg gttcatatag cccagcatta gcacttcgcc
    16201 agataccgcg tgttgcacaa tcaccggcat cagaccgtcg gttttttccc agtccagttc
    16261 gcgacgttgt tgttctgtta acatatcctg atctccacgc cctgtgttgc caggtacgct
    16321 tttaattcac caatattgat tatttgtttg tggaatacgg aagctgccag cgcaccgtca
    16381 acgtcggcat cgcggaaggc ttcgagaaag tgttccatgg tgcctgcgcc accggaggca
    16441 atcagcggga cgtggcaaac ttcacgcact tttttcagct gttcgaggtc gtagccgtta
    16501 cgcacgccgt cctgattcat catgttgagg acgatttctc cggcaccgcg tttttgcact
    16561 tcctgcaccc aatcgagtgt ttcccactg
//