LOCUS       EU549863               14063 bp    DNA     linear   BCT 11-JAN-2012
DEFINITION  Escherichia coli serogroup O2 O-antigen gene cluster, partial
            sequence.
ACCESSION   EU549863
VERSION     EU549863.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14063)
  AUTHORS   Fratamico,P.M., Yan,X., Liu,Y., DebRoy,C., Byrne,B., Monaghan,A.,
            Fanning,S. and Bolton,D.
  TITLE     Escherichia coli serogroup O2 and O28ac O-antigen gene cluster
            sequences and detection of pathogenic E. coli O2 and O28ac by PCR
  JOURNAL   Can. J. Microbiol. 56 (4), 308-316 (2010)
   PUBMED   20453897
REFERENCE   2  (bases 1 to 14063)
  AUTHORS   Fratamico,P. and Yan,X.
  TITLE     Escherichia coli serogroup O2 O-antigen gene cluster
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 14063)
  AUTHORS   Yan,X. and Fratamico,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2008) Microbial Food Safety Research Unit, ERRC,
            USDA, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA
REFERENCE   4  (bases 1 to 14063)
  AUTHORS   Yan,X. and Fratamico,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2012) Microbial Food Safety Research Unit, ERRC,
            USDA, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA
  REMARK    Amino acid sequence updated by submitter
FEATURES             Location/Qualifiers
     source          1..14063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O2"
     misc_feature    <1..>14063
                     /note="O-antigen gene cluster"
     gene            <1..1152
                     /gene="rmlB"
     CDS             <1..1152
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6 dehydratase"
                     /protein_id="ACD75801.1"
                     /translation="YFYYQSRAVYFTVCSLAIWNKKVKILVTGGAGFIGSAVVRHIIN
                     NTQDSVVNVDKLTYAGNLESLAEVSDSERYSFEHADICDAEAMARIFSQHQPDAVMHL
                     AAESHVDRSITGPAAFIETNIVGTYVLLEAARNYWSGLDDEKKKNFRFHHISTDEVYG
                     DLPHPDEVNNNEALPLFTETTAYAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSN
                     NYGPYHFPEKLIPLVILNALEGKALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGE
                     TYNIGGHNEKKNIDVVLTICDLLDEIVPKEKSYREQITYVADRPGHDRRYAIDADKIS
                     RELGWKPQETFESGIRKTVEWYLANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            1152..2051
                     /gene="rmlD"
     CDS             1152..2051
                     /gene="rmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /protein_id="ACD75802.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTNYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPDFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGSGDAPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALKKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            2110..2985
                     /gene="rmlA"
     CDS             2110..2985
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="ACD75803.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEDFIGG
                     DDCALVLGDNIFYGHDLPKLMEAAVNKESGATVFAYHVNDPERYGVVEFDNNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     IKNQYGQYLLKMISE"
     gene            3000..3413
                     /gene="ftdA"
     CDS             3000..3413
                     /gene="ftdA"
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-hexose isomerase"
                     /protein_id="ACD75804.1"
                     /translation="MMDIKLISLQKHGDERGALIALEEQRNIPFEVKRIYYILETLNG
                     VRRGFHAHKVTRQLAIVVKGACKFHLDNGKETKQVELNDPTIALLIEPYIWHEMYDFS
                     DDCVLLVIADDFYKESDYIRNYDDFIRRVNSIENS"
     gene            3400..3867
                     /gene="ftdC"
     CDS             3400..3867
                     /gene="ftdC"
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like protein"
                     /protein_id="AEX59993.1"
                     /translation="MRIHKLSDVQTTSIGDGTTIWQFVVILKGAVIGNNCNICANTLI
                     ENNVVIGNNVTVKSGVYIWDGVKIEDNVFIGPCVAFTNDKYPRSKVYPDEFLQTIIRK
                     GASIGANATILPGIEIGEKAIVGAGSVVTKNVPPCAIVVGNPARFIKWVEDNE"
     gene            3860..4966
                     /gene="ftdB"
     CDS             3860..4966
                     /gene="ftdB"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="ACD75805.1"
                     /translation="MNKIDFLDLFAINQRQHKELVSAFSRVLDSGWYIMGEELEQFEK
                     EFAEYCGVKYCIGVANGLDALILVLRAWKELGYLEDGDEVLVPANTYIASILAITENK
                     LVPVLVEPDIETYNINPALIENYITEKTKAILPVHLYGLLCNMPEISAIARKYNLLIL
                     EDCAQAHGAIRDGRKAGAWGDAAGFSFYPGKNLGALGDAGAVTTNNAELSSTIKALRN
                     YGSHKKYENIYQGLNSRLDELQAALLRVKIHTLPEDTAIRQRIAEKYIREIKNPAITL
                     PVYEGQGAHVWHLFVVRIANREKFQSYLLEKGIKTLIHYPLPPHKQQAYQNMSSLSLP
                     ITEQIHDEVISLPISPVMSEDDVNYVIKMVNDYK"
     gene            4966..6228
                     /gene="wzx"
     CDS             4966..6228
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="ACD75806.1"
                     /translation="MKKFLQVTILSAIYTFIKMIAGFIIGKVVAIYTGPSGVAMLGQV
                     QSLITIVAGTTSAPVSTGLVRYTAENWQEGQEACAPWWRACLRVTLFLFLLIIPVVII
                     LSKNISELLFSDGQYTWLIIFACCILPFSIINTLIASVLNGQQFYKQYILVGMFSVFI
                     STMFMILLIVAYNLKGALIATAINSAIAGLVLVLFCLNKSWFRFKYWWGKTDKDKIIK
                     IIHYTLMALVSVISMPTALMCIRKILIAKTGWEDAGQWQAVWKISEVYLGVVTIALST
                     YFLPRLTIIKTSFLIKKEVNSTILYIISITSFMALSIYLFRDLVITVLFTEQFRSARE
                     LFLLQLIGDVIKIAGFLYAYPLQSQGHTKLFISSEVIFSMLFIITTYIFVVNYGVHGA
                     NISYVITYSLYFVFAFVFTNFINVRRNN"
     gene            6323..7294
                     /gene="wegP"
     CDS             6323..7294
                     /gene="wegP"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="ACD75807.1"
                     /translation="MNYGHDVSFVVPDNRTNPYYATTAKIVTSKSSQNRVKILRIIKN
                     YYNLWRKCIELNPDAVVASFHLTAYLVALLPITRRKKYYYIQAYEVNFFDNIIWKLIA
                     GLTYYLPLKKILNSPNLLPHKHDDFIGVVPAGVDLNVFYPKPSNRLLNGHTSIGIIGR
                     KEKHKGTSEIISVLCSLENKAGIIINIAIYLEEVDKQRLIAAGFQVNFFPITSDLELA
                     SFYRSNDIMIAVGLIEDGAFHYPCAESMACGCLVISNYAPLTETNSVLKLVKFDACKL
                     GEAINLCLNLDLEEKSKEIQSNISVLNKYDWKIVGETFNSLLLDANK"
     gene            7315..7872
                     /gene="rmlC"
     CDS             7315..7872
                     /gene="rmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="ACD75808.1"
                     /translation="MNIVKTDIPDLIVLEPKVFSDERGFFMESYNQIEFEKAIGRHVN
                     FVQDNHSKSSKGVLRGLHYQLAPYAQAKLVRCVVGQVFDVAVDLRKNSPTFKKWFGIT
                     LSAENKRQLWIPEGFAHGFLVTSDEAEFIYKTTNYYAPGHQQAIIYNDPILNIDWPFC
                     SSALSLSQKDQEAKLFSELLDSELF"
     gene            7941..8903
                     /gene="wzy"
     CDS             7941..8903
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="ACD75809.1"
                     /translation="MTENSPKYKHDKSLISFIYLFFIFTLIVGFIIANTQFLGRSRDY
                     DNYIQIFSGKEGEGVLELFYRGLMLITTSYETIIFIILTCSFFIKARFLANYSRNFSG
                     LTLFFIYYASVALWVLDYTQFRNGLCISILMFSVYYLFINKPTYFYFSVLCAIATHWS
                     ALPFLLLYPFVYSTKIRRLGYFCFSILVLIAISGEGKEIISFIRNFGVGQKIGNEAGV
                     NLINSLSLTAISWFIISYISSIGNERRNLRLFFCYGVMQYVTFSLFSLPVMAFRILEM
                     YFFLMLTIGVFIKQKKNYYFVFCKVLILLYLTYYYHMVFGVINV"
     gene            8896..9783
                     /gene="wegQ"
     CDS             8896..9783
                     /gene="wegQ"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="ACD75810.1"
                     /translation="MCKAKVLAIIVTYNPEIIRLTECINSLAPQVERIILVDNGSNNS
                     DLIKNISINNLEIILLSENKGIAFAQNHGVKKGLEAKEFDYLFFSDQDTCFPSDVIEK
                     LKSTFTKNNKKGKNVACASPFFKDHRSNYMHPSVSLNIFTSTKVICSEVDDDLYPSHV
                     IASGMLMSREAWRVVGPFCEKLFIDWVDTEWCWRALANNMIIVQTPSVIISHELGYGQ
                     KIFAGRSVTIHNSFRNFYKIRNAIYLMLHSNYSFKYRYHAFFHATKNVVFEILYSKEK
                     LNSLKVCFKAVRDGMFNNF"
     gene            9814..10890
                     /gene="wegR"
     CDS             9814..10890
                     /gene="wegR"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosy ltransferase"
                     /protein_id="ACD75811.1"
                     /translation="MEENNMKTVAVVGTVGVPACYGGFESLVQNLIDYQSDGIQYQIF
                     CSSKKYDKKFKNYKNAELIYLPINANGVSSIIYDIMCLIICLFKRPDVVLILGVSGCL
                     FLPIYKLFSKSKIIVNIDGLEWRRNKWGTFAKKFLKISEAISIRIADIIISDNQAIAD
                     YVENKYKKKSVVIAYGGDHATNLSTPIDNDQKKEGYYLGLCRIEPENNIEMILNAFIN
                     TDKKIKFMGNWDNSEYGRQLKKYYSNYPNITLLEPNYNIEELYKLRKNCLAYIHGHSA
                     GGTNPSLVEAMHFNIPIFAFDCDFNRYTTNNLAHYFNDSEQLSLLAESLSFGNLKCRV
                     LDLKNYAEDMYNWRHIAAMYESIY"
     gene            11070..11666
                     /gene="yhhI"
     CDS             11070..11666
                     /gene="yhhI"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="ACD75812.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFDHGIPAHDTIARVVSCISPAKFHECFINWMRDCYSSYD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVIGQIKTDEKFNEVTAIPELLN
                     IWILKEKLSQLMRWVARKILQRRYKNGGGDYLFAVKVN"
     CDS             11251..11562
                     /gene="yhhI"
                     /note="ORF14"
                     /codon_start=1
                     /transl_table=11
                     /product="H repeat-associated protein"
                     /protein_id="AEX59994.1"
                     /translation="MVILIMVFLLTIPLPELYPVSVLQNFTSALLTGCVIVILHMIKT
                     SSQLMEKHSGILMTRVAARERFMSLVRSQQCTVWSSDRSRRMRNSMRSQLSQNFLTYG
                     Y"
     CDS             11853..12206
                     /note="ORF15"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="ACD75813.2"
                     /translation="MAVSFRSIIAEQKKEPEMTVRYYISSAGLTAEKFATVIRNHWHV
                     KNKLHWRQGVVMNEDHCKIRRGIAAELFSGIRYIGINILTKHKVFKAGLRCKMRRADM
                     DRDYLASVFAGSWLS"
     gene            12275..13903
                     /gene="wegW"
     CDS             12275..13903
                     /gene="wegW"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="ACD75814.1"
                     /translation="MNFIIIIRMILPFCIFLSILLYSGRVDSVFLFSIEYTFISVAIF
                     LIKRKFVSNILWVIYLSILGVQAASLFSSGQYLMPLALSNAAEIESLGIGEVIKVFFV
                     FILFIIISFITLPSGALTERIKYYKFNLLCIVLLLVCSFISAGPVYAFYETAKLFYLQ
                     QTFKPSKKIGDAGKFFLKTSVFINPIENNYSFKGRNVIVIFAEGFSSEIMGNAKPRAF
                     SVTPNLDKLSTEALSFKNYYNHTAATFRGLRGQLTSGYQFRDGLTDAGTGIAQLSKAE
                     INNIYLHRQTSLPDILKEYGYKSYFIASTEKNSPLNTMLKTLNFDKVLGMGDFIGYQR
                     DRMTDKQTFNALQYFLRSQENKKGPFFIGVYPSGTHHGQDSPNEKYFDGNNPLYNKFY
                     NYDFQLGKFIDFFRRSSFYNNTLLIITSDHSTFPSTEYKKAFNSDSRYFVGEIPFLVI
                     GGDITPEILDADGKNSLSFAPTILHMLGIQYSMNYFLGCSLIDKECTSPFSHLSAIGS
                     DYFVTSKENHVELIPNNNDSELIKLAESFYNISG"
ORIGIN      
        1 tacttctatt atcaatctcg agcagtctat ttcacagtat gctctctggc tatatggaat
       61 aaaaaagtga agatacttgt tactggtggc gcaggcttta ttggttctgc tgttgttcgt
      121 cacataataa ataatacgca agatagtgtt gttaatgtcg ataaattaac atacgccgga
      181 aacctggaat cgctcgctga agtttctgat tctgagcgtt attcatttga gcatgcagat
      241 atctgcgatg ccgaagcgat ggcgcgtatt ttttcacaac accagccaga tgcggtgatg
      301 caccttgcgg ctgagagcca cgttgaccgc tcaataactg gcccggcggc attcattgaa
      361 actaatattg taggtactta cgtactttta gaagctgcgc gcaattattg gtctggtctg
      421 gatgatgaaa agaaaaaaaa cttccgcttt catcatattt ctactgatga agtatatggt
      481 gacttacccc atccggatga agtaaataat aacgaagcgt taccgctatt tacggaaacg
      541 acagcctacg cgccaagtag cccgtattct gcttctaaag cttccagcga tcatttggtt
      601 cgcgcatgga aacgtacgta tggtttaccg accattgtga ctaattgctc gaacaactat
      661 ggtccgtatc acttcccgga aaagcttatt ccattggtta ttcttaatgc actggaaggt
      721 aaggcattac ctatttatgg caaaggggat caaattcgcg actggttgta tgtagaggat
      781 catgctcgtg cgttatatac cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt
      841 ggcggacaca acgaaaagaa aaacatcgat gttgtgctga ctatttgtga tttgttggat
      901 gagattgtac cgaaagagaa atcttatcgt gagcaaatta cttatgttgc tgatcgccca
      961 gggcatgatc gccgttatgc aattgatgcc gataaaatta gccgcgaatt gggctggaaa
     1021 ccacaggaaa cgtttgagag cgggattcgc aaaacggtgg aatggtatct ggctaataca
     1081 aattgggttg agaatgtgaa aagcggtgct tatcagtcat ggatcgaaca aaactatgag
     1141 ggccgtcagt aatgaatatc ctgcttttcg gcaaaacagg gcaggtgggt tgggaactgc
     1201 agcgtgctct ggcgccgctg ggtaatctga tcgctcttga tgttcactcc actaattatt
     1261 gtggagattt cagcaacccc gaaggtgtgg cagaaaccgt caaaaaaatt cgtcctgacg
     1321 ttattgttaa tgctgctgct cacactgcag tagataaagc agaatcagaa ccggatttcg
     1381 cacaattact taacgcgaca agcgtcgaag cgattgcaaa agctgctaat gaagtcgggg
     1441 cctgggttat acactactct actgattatg ttttcccagg cagtggtgac gcgccatggc
     1501 tggaaacgga tgcaacagca ccgctaaatg tttacggtga aacaaaatta gctggggaaa
     1561 aggcattaca agaacattgc gcaaagcatc ttattttccg taccagctgg gtatacgctg
     1621 gtaaaggaaa taactttgct aaaacgatgt tgcgtttggc aaaagaacgc gaagaactgg
     1681 ctgtgataaa cgatcagttt ggcgcaccaa caggtgctga attgctggct gattgcaccg
     1741 ctcatgccat tcgcgtggca ttaaaaaaac cagaagtcgc tggcttgtac catctggtag
     1801 caagtggcac aacaacctgg cacgattatg ctgcgctggt ttttgaagag gcgcgcaaag
     1861 cagggattaa tcttgcactt aacaaactta acgccgtgcc aacaacggcc tatcccacac
     1921 cagcccgtcg accccataac tctcgcctca atacagaaaa gtttcagcag aactttgcgc
     1981 ttgtcttgcc tgactggcag gtgggcgtga aacgtatgct caacgaatta tttacgacta
     2041 cggcaattta acaaattttt gcatctcgct catgatgcca gagcgggatg aattaaaagg
     2101 aatggtgaaa tgaaaacgcg taaaggtatt attctggctg gtggttccgg cactcgtctt
     2161 tatcctgtga cgatggcagt gagtaaacaa ttgctgccga tttatgataa gccgatgatt
     2221 tattatccgc tttcaacgct tatgttagcg ggtattcgcg atattcttat tattagtacg
     2281 ccacaggata caccgcgttt ccaacaatta ttgggggacg ggagccagtg gggtcttaat
     2341 ctacagtata aagtacaacc gagtccggat ggcctggcgc aagcgtttat tattggcgaa
     2401 gactttattg gtggtgatga ttgtgcactc gtacttggcg ataatatctt ctatggacac
     2461 gacttgccga aattgatgga agctgctgtt aacaaagaaa gcggtgcaac ggtatttgct
     2521 tatcacgtta atgatcctga acgctatggt gtcgtggagt ttgataataa cggtacggca
     2581 attagcctgg aagaaaaacc gctggagcca aaaagcaact atgcggttac tgggctttat
     2641 ttctatgaca atgacgttgt ggaaatggct aaaaacctta agccttctgc ccgtggcgaa
     2701 ctggaaatta ccgatattaa ccgtatttat atggaacaag gacgtttgtc tgtagccatg
     2761 atggggcgtg gctatgcatg gttggataca gggacgcatc aaagccttat tgaagcaagt
     2821 aacttcattg caacaattga agagcgtcag ggattaaagg tatcttgccc ggaagagatt
     2881 gcttaccgta aagggtttat tgatgccgag caggtgaaag tattagccga accgcttatc
     2941 aagaatcaat atggtcaata tttgctgaaa atgatcagcg aatagtatat gggaactcaa
     3001 tgatggatat taaattaatc tctttgcaaa aacatgggga tgagcgcggt gcattaattg
     3061 ctcttgaaga gcaacgaaat atacctttcg aagtcaaaag aatatattac atacttgaga
     3121 ctcttaatgg agtaagacgc ggatttcatg cgcacaaggt tactcgtcag ttagctattg
     3181 tagtcaaggg agcttgtaaa tttcatctgg ataatggtaa agaaacaaag caggtggaac
     3241 ttaatgatcc aacaattgcg ttgctgatag aaccctatat atggcatgaa atgtatgatt
     3301 ttagtgatga ttgtgtgctg cttgtaattg cggatgattt ctataaagag tctgattata
     3361 tccgcaatta tgatgatttt attagaagag taaattcaat tgagaattca taagctaagt
     3421 gacgtccaga caacatcaat tggtgatgga acaactatct ggcagtttgt tgtgatacta
     3481 aaaggtgctg taattggtaa taattgcaac atctgtgcaa ataccttaat tgaaaataac
     3541 gttgtaattg gtaacaatgt cacagtcaaa agcggtgtgt atatttggga tggcgttaaa
     3601 atagaggata atgtttttat tggtccttgt gtagcattta caaatgataa gtatcctcgc
     3661 tctaaagtct atcctgatga atttttgcaa acaataatac gcaaaggagc atcaataggt
     3721 gctaacgcaa ccatcctgcc aggaattgaa attggtgaaa aagcaatcgt tggtgcgggg
     3781 agtgttgtaa ccaaaaatgt accgccatgc gcaatagtag taggtaatcc agctcgattt
     3841 attaaatggg tagaggataa tgaataaaat tgatttttta gatctttttg caattaacca
     3901 gcgacagcac aaagaattag tctctgcgtt tagtagggtg ctagattctg gttggtatat
     3961 catgggcgaa gaacttgagc agttcgagaa agagttcgca gaatactgtg gagttaagta
     4021 ttgcattggt gtagcaaatg gccttgatgc gttgatacta gtattgaggg catggaaaga
     4081 acttggctat cttgaagacg gtgacgaggt attagtaccg gcaaatacat atattgcttc
     4141 tattcttgct ataacagaga acaaacttgt tcctgttctt gttgaaccag atatagaaac
     4201 ttataatatt aatcctgctt taattgaaaa ttacattacg gaaaaaacta aagcaatatt
     4261 accggttcac ttatatggtc tattgtgcaa tatgccagaa attagtgcaa tcgccagaaa
     4321 atataatctg ttgattcttg aagattgtgc acaagcacat ggtgcaatac gtgatggtcg
     4381 caaagctgga gcttgggggg atgctgcagg atttagtttt tatccaggaa aaaaccttgg
     4441 agctttgggg gatgcgggag ctgttactac aaataatgca gaattatcct caactataaa
     4501 agctttgcga aattatgggt cacataagaa atatgaaaat atttatcagg gattgaatag
     4561 tcgattggat gaactgcaag cagccttatt gcgtgtaaaa atccatacat taccggaaga
     4621 tactgcgatt cggcaaagga ttgctgaaaa atatattcgt gaaataaaaa accctgcgat
     4681 tacgttacca gtgtacgaag gccaaggtgc gcacgtttgg catttatttg tagtaagaat
     4741 cgctaatcgt gaaaaattcc agtcatactt attagagaag ggtatcaaaa ccttaattca
     4801 ctatccatta ccaccccata agcagcaagc atatcaaaat atgtctagcc ttagccttcc
     4861 aattactgag caaattcatg atgaagtcat ttctttacct ataagtccgg taatgagtga
     4921 agatgatgtc aattatgtaa tcaaaatggt caatgattac aagtaatgaa aaaatttctt
     4981 caggtaacta tattatccgc tatctataca ttcattaaaa tgattgcggg ttttatcatc
     5041 ggtaaggtag tagcaattta tacagggcca tcaggggtag caatgcttgg ccaagtgcaa
     5101 agtttaatca caatagttgc aggtactacc tctgcacctg taagcactgg ccttgttcga
     5161 tatactgcgg aaaattggca agaaggacaa gaagcatgcg cgccatggtg gcgcgcatgc
     5221 ttaagggtta ctctgttttt attcttgctt attattcccg ttgttattat attgtcgaaa
     5281 aatattagtg agttactttt tagcgatgga caatacacat ggttaatcat tttcgcatgt
     5341 tgtatattgc cattctccat tataaataca ttgatcgctt cagttttaaa tggtcaacaa
     5401 ttttataagc aatatatatt ggttgggatg ttttctgtat tcatttctac tatgtttatg
     5461 attttgttga ttgtagctta taatcttaaa ggtgcattga ttgccacagc tataaatagt
     5521 gctattgctg gtcttgtatt ggttttattt tgtctcaata aatcttggtt tagatttaaa
     5581 tattggtggg gtaaaacgga taaagacaaa attataaaaa ttattcatta tactctgatg
     5641 gctctggttt ctgttatctc catgcctaca gcattgatgt gtattagaaa aatattgatt
     5701 gctaaaactg gttgggagga tgcagggcaa tggcaggccg tatggaagat atctgaggtt
     5761 tatcttggtg ttgtgacaat tgctttgtca acatatttct taccaagatt gacaattata
     5821 aaaacaagtt tccttataaa aaaagaagta aatagtacta tattatacat aatatctatt
     5881 acttcattca tggcgttgag tatctattta ttccgcgatt tggtaataac agttttattt
     5941 actgaacagt ttcgctcagc tcgtgaatta tttttattac aacttatagg ggatgtaata
     6001 aaaattgctg ggtttcttta tgcataccct cttcaaagtc aggggcatac taaactattc
     6061 atcagttcag aagtgatttt ttctatgctc tttatcatta ccacctatat ttttgttgta
     6121 aattatggag tacatggtgc taacataagt tatgtcatta catatagttt atattttgtg
     6181 tttgcatttg tgtttactaa ttttattaat gttagaagaa ataattaaaa acagaggttg
     6241 aattttgaaa ataattatac ctgtcttagg atttggcagg gctggtggtg aaagagttct
     6301 ttctaagctg gcaactgaat tgatgaatta tggacatgat gtaagttttg ttgttccaga
     6361 taatagaact aatccatatt atgctaccac agcaaaaatt gtcacgagta aatctagtca
     6421 aaaccgtgta aaaatattga gaatcattaa aaattactat aatctgtggc gtaaatgcat
     6481 agagttaaat cctgatgctg tagttgctag ttttcatttg actgcctatc ttgtcgcatt
     6541 attaccaatc acccgtcgta agaaatatta ttatattcag gcgtatgaag ttaatttttt
     6601 tgataatata atatggaaat taatagcggg tttaacatat tatttaccgc ttaaaaaaat
     6661 actaaatagt cctaatttgc ttcctcataa acatgatgat tttataggag tagttcctgc
     6721 aggagtagat ttaaacgttt tctatccgaa accatcaaat aggttattaa atggtcacac
     6781 atcaataggg attattggta gaaaagagaa gcacaaagga actagcgaaa ttatttcagt
     6841 attgtgttca ctggaaaata aagctggaat tataatcaat attgcgatct atcttgaaga
     6901 agttgataag cagcgtttaa tcgctgccgg gtttcaggtt aatttttttc cgattacttc
     6961 tgatttagaa ttggcatcct tttatcgaag caatgacatc atgattgctg ttgggttaat
     7021 tgaagatggc gctttccatt atccttgtgc tgaatcaatg gcttgtggtt gtcttgttat
     7081 ttcaaattat gcgccactta ctgaaactaa cagtgtactt aaattagtca agtttgatgc
     7141 ttgcaaactt ggtgaagcaa ttaatctttg tctcaatctt gacctagaag aaaaaagcaa
     7201 agaaatccaa tctaatattt ctgtgttgaa taaatatgac tggaaaattg ttggtgaaac
     7261 tttcaatagt ttattgttag atgcaaataa atagtatacg ttgatgggga aaatatgaat
     7321 attgttaaaa ctgatattcc agatctgatc gttcttgaac caaaagtgtt tagtgatgaa
     7381 cgcggctttt ttatggagag ttataatcag attgaatttg agaaggcaat aggaaggcac
     7441 gtaaattttg ttcaggataa tcattcaaaa tctagtaaag gcgtactacg tgggttgcat
     7501 tatcaattag caccgtatgc acaggctaaa ttagttcgat gtgttgtagg tcaggtattt
     7561 gatgttgctg ttgatcttag aaaaaattca ccaacgttca aaaaatggtt tggaataacc
     7621 ctttccgcag aaaataaacg acaattatgg atacccgaag gatttgctca tggtttcttg
     7681 gtgaccagtg atgaagctga gttcatttat aagacaacta actactatgc tcctggtcat
     7741 cagcaagcaa ttatttacaa tgatcctatt ttaaacatcg attggccttt ctgcagtagt
     7801 gctctgtcat tatcacaaaa agatcaagaa gcaaaattat tttcagaatt attggacagt
     7861 gaactgttct aataaagtgt gccaccttat ccgtctgaag gataggtggt tgcttatatt
     7921 tttttgagta tgtttgtata atgacagaaa atagtccgaa atataaacac gataaaagct
     7981 taataagttt tatctactta ttttttatat ttacacttat tgtaggcttt attatcgcaa
     8041 atacccagtt tttggggcga agtagagact atgataatta tatacagatc ttttctggta
     8101 aagaagggga gggggttctt gaattatttt atcgcggatt gatgttaata acgaccagct
     8161 atgaaactat catttttata attttaacat gttctttttt tataaaggca aggtttctcg
     8221 ctaactattc gcgtaatttt tcaggcttga ccttattctt tatttattat gcaagcgttg
     8281 cactttgggt tttagattat actcaattca gaaatggtct atgtatttcc attttaatgt
     8341 tttccgtata ctatttattt ataaataaac cgacttattt ttatttctcg gtattatgtg
     8401 caattgcaac tcattggtct gctttgcctt ttttgctttt atatcctttt gtctattcaa
     8461 caaaaataag acgccttggt tatttttgtt tcagtattct tgttttgatt gcgatctcag
     8521 gagaaggaaa agagatcata tcttttataa gaaattttgg agtgggacaa aaaataggaa
     8581 atgaagctgg tgtaaattta ataaattcat tatcccttac cgctatttcc tggtttatta
     8641 ttagttacat atcaagcatt ggaaatgaaa ggagaaattt aaggcttttc ttttgttatg
     8701 gtgtcatgca atacgtgact tttagccttt tctctctacc tgttatggct ttccgtattt
     8761 tggaaatgta ttttttcctt atgctaacca ttggggtgtt tattaagcaa aaaaagaatt
     8821 attattttgt tttttgcaaa gtgttaattt tattgtatct aacatactat tatcatatgg
     8881 tctttggagt gattaatgtg taaggctaag gtgttggcta taattgttac ttacaacccg
     8941 gaaattattc gattgacgga atgtattaac tctttagccc cacaagttga gagaataatt
     9001 cttgtagata atggctcaaa taatagtgat ttgataaaaa atatcagtat taataacctt
     9061 gaaattattt tactttcgga aaacaaaggc attgcatttg ctcagaacca tggtgttaag
     9121 aagggcctgg aagcaaaaga gtttgactat ttatttttct cagatcagga tacttgcttt
     9181 cctagcgatg ttattgaaaa acttaagagt acatttacga aaaataataa aaaaggtaaa
     9241 aatgttgctt gtgcttctcc tttttttaaa gaccatcgtt caaattatat gcatccgtca
     9301 gtcagcctaa atatttttac gagtacaaaa gttatatgta gtgaagtaga cgatgatctt
     9361 tatccctcgc atgttattgc ttctgggatg ttaatgtctc gtgaagcatg gcgcgtcgtc
     9421 ggaccatttt gtgaaaaact ctttatagac tgggttgata cagaatggtg ttggcgtgca
     9481 ttagctaata atatgattat tgttcagaca ccatcagtca tcatttctca tgaacttggg
     9541 tatgggcaga aaatttttgc tggtcgatct gttacaatac ataattcttt cagaaatttt
     9601 tataaaatac gcaatgcaat atacttaatg ctgcattcaa attatagctt caagtatcgt
     9661 tatcatgctt tttttcatgc gacaaagaat gttgtatttg aaattttata ttcgaaagaa
     9721 aaattaaatt cactgaaggt ttgttttaaa gctgtacgtg atggtatgtt caataatttt
     9781 taatacgaaa atagttaggc tcaaggtgtt taaatggaag aaaataatat gaagacggtc
     9841 gctgtagttg gcacagtggg tgttcctgct tgttatggtg ggttcgaatc acttgttcag
     9901 aatctaattg attatcaatc tgatggtata caatatcaga tattttgctc ttcaaaaaaa
     9961 tatgataaaa aatttaaaaa ttataaaaat gcagaattaa tctatttgcc gataaatgcc
    10021 aatggcgtct ctagcataat ttatgatatt atgtgtttaa ttatttgttt attcaaaagg
    10081 ccagatgttg ttttaatatt gggggtgtct ggttgtttat ttctaccaat ttataaacta
    10141 ttttcaaaat caaagattat tgtcaatatt gatgggcttg aatggcgtag aaataaatgg
    10201 ggaacgtttg ctaagaaatt tcttaaaata tctgaggcga tatctattag aatagctgat
    10261 attatcattt cagataatca agcaatagct gattatgtgg aaaataagta caagaaaaaa
    10321 agtgtagtta tagcttatgg cggagatcat gccactaatc ttagtacacc gatagacaat
    10381 gatcaaaaaa aagaaggtta ttatttgggg ctttgtagga tagagcctga gaataatata
    10441 gaaatgattc tgaatgcctt cattaataca gataaaaaaa ttaaatttat gggtaattgg
    10501 gataacagcg agtatggacg ccagctaaaa aaatattatt caaactatcc aaatatcacc
    10561 ctactagaac ctaactataa tattgaagag ctttataaac taagaaaaaa ttgtcttgca
    10621 tacattcatg gacactcggc tggtggaaca aacccttctt tagttgaagc gatgcatttt
    10681 aatattccta tttttgcttt cgattgtgac tttaatcgtt acacaactaa caatttagct
    10741 cattacttta atgattctga acaacttagc ttattagcag aaagtttgtc ttttggaaat
    10801 cttaaatgtc gagtattaga tttaaaaaat tatgctgaag atatgtataa ctggaggcat
    10861 atagctgcta tgtatgaatc tatttattaa acgcattaac aataatataa ttgaccttat
    10921 atagcaggga aagatcacgt aacgctgctt ttttgtacta aataattcgc attttatgtt
    10981 taaaaattga gatatccctt attacctaaa gctgtttttt attgcttata ccatgatcaa
    11041 atactcctta cataattaag gagaacaaaa tggaacttaa aaaattgatg gaacatattt
    11101 ctattatccc cgattacaga caagcctgga aagtggaaca taaattatcg gatattctac
    11161 tgttgactat ttgtgccgtt atttcgggtg cagaaggctg ggaagatata gaggattttg
    11221 gggaaacaca tctcgatttt ttgaagcaat atggtgattt tgatcatggt attcctgctc
    11281 acgataccat tgccagagtt gtatcctgta tcagtcctgc aaaatttcac gagtgcttta
    11341 ttaactggat gcgtgattgt tattcttcat atgataaaga cgtcatcgca attgatggaa
    11401 aaacactccg gcattcttat gacaagagtc gccgcaaggg agcgattcat gtcattagtg
    11461 cgttctcaac aatgcacagt ctggtcatcg gacagatcaa gacggatgag aaattcaatg
    11521 aggtcacagc tatcccagaa cttcttaaca tatggatatt aaaggaaaaa ttatcacaac
    11581 tgatgcgatg ggttgccaga aagatattgc agagaagata caaaaacggg ggaggtgatt
    11641 atttattcgc tgtaaaagta aattaggggc ggcttaataa agccattgag ggataatccc
    11701 cgctgaaaga attaaataat ccaaagcatg gcagttacga aataagtgaa cagagtcacg
    11761 gcagagaaga catccgtctt catattgttt gcgatgttcc tgataaactt attgatttca
    11821 cgttttaatg gaaaggactg ataaaattat gcgtggcagt ctcctttcgg tcaataatag
    11881 cagaacaaaa gaaggagcct gaaatgacgg tcagatatta tatcagttct gcgggtttaa
    11941 cagcagagaa gtttgcgaca gtgattcgaa atcactggca cgtgaaaaat aagctgcact
    12001 ggcgtcaggg cgtggtaatg aatgaagacc actgcaaaat aagaagagga attgcagcag
    12061 aattattttc agggatcagg tatatcggca ttaatatttt gacgaaacat aaagtattca
    12121 aggctgggtt aagatgtaag atgagaagag cagatatgga cagagactac ctcgcatcag
    12181 tttttgcggg gagctggctt tcgtaatctt gccctggtga tgcaatgctg aattaatagc
    12241 attaaagatt ttaaataaaa tggtttaact caacatgaac tttataatta ttattagaat
    12301 gattttgcct ttctgtattt ttttgagtat tttactttac tctgggaggg tggatagtgt
    12361 ttttttattt tctattgaat acacatttat aagtgtggca atctttctga taaagagaaa
    12421 gtttgtaagt aatattttat gggtaattta tctttccatt ttaggtgtgc aggcagcgtc
    12481 gctatttagt tcaggacaat atttgatgcc tcttgccttg tcgaatgctg cagaaattga
    12541 atccttgggt attggtgagg taattaaagt tttttttgtt tttattttgt ttattataat
    12601 ttcattcatt accttgcctt caggggctct aaccgagcgt attaaatatt ataaattcaa
    12661 ccttctctgt atagtattat tgctcgtgtg ttctttcata agtgcgggac cagtatatgc
    12721 tttttatgaa acagcaaaat tgttctattt acagcaaacc tttaagccta gtaagaagat
    12781 cggagatgca gggaaattct ttttaaaaac aagtgttttt attaacccta tagaaaacaa
    12841 ctacagtttc aaaggccgta atgttattgt aatatttgct gagggatttt catctgaaat
    12901 tatgggtaat gccaaaccaa gagcattttc agttacgcct aatttagata aattatctac
    12961 tgaagcacta agtttcaaaa attattataa ccatacagct gcaacattcc gaggattaag
    13021 aggtcagtta acttctggtt atcaatttcg agatggttta actgacgctg gcacaggtat
    13081 cgcacaatta tctaaagcag aaattaataa tatttacctt caccgtcaaa catctttacc
    13141 tgatatatta aaagagtatg gatataaaag ttattttata gcttccaccg aaaaaaatag
    13201 cccactcaat acaatgttga agacacttaa ttttgataag gttcttggta tgggtgattt
    13261 tattggttat caacgagaca gaatgacgga taagcaaaca tttaatgcac tgcaatattt
    13321 tttacgttct caggaaaata aaaaaggacc cttctttatt ggtgtctatc catctggtac
    13381 tcatcatggt caagatagcc caaatgaaaa atattttgat ggtaacaacc ctctctacaa
    13441 taagttctat aactatgatt ttcagttagg gaaatttatt gacttctttc ggcgcagttc
    13501 attttataat aatacattat taataataac gtcagatcat tcaacatttc cttcgacaga
    13561 atataaaaaa gcatttaata gcgattctcg ttactttgta ggcgaaatac catttctggt
    13621 tattggagga gatattactc ctgaaatttt ggatgcagac ggtaaaaatt cattaagctt
    13681 tgcgccaaca atattgcata tgttagggat tcagtattca atgaactatt ttttgggatg
    13741 ttctcttatt gataaggaat gcacatcgcc attttcacat ctttctgcta ttggtagtga
    13801 ctatttcgtt acaagcaaag aaaatcatgt tgaactaata ccaaacaata atgatagtga
    13861 attaataaag cttgcagaaa gtttctataa tatcagtgga taagcagatg gttgggcaaa
    13921 aaataatcgc ctcatctaat tatgaaacat acgactgtaa aataagatat aaattaagct
    13981 attctttatc ttaaataagc cgcaaccccg cggtgaccac ccctgacagg agtaaacaat
    14041 gtcaaagcaa cagatcggcg tcg
//