<pre>LOCUS       GU299793               12075 bp    DNA     linear   BCT 08-JUN-2010
DEFINITION  Escherichia coli strain G1630 O antigen gene cluster, complete
            sequence.
ACCESSION   GU299793
VERSION     GU299793.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12075)
  AUTHORS   Li,D., Liu,B., Chen,M., Guo,D., Guo,X., Liu,F., Feng,L. and Wang,L.
  TITLE     A multiplex PCR method to detect 14 Escherichia coli serogroups
            associated with urinary tract infections
  JOURNAL   J. Microbiol. Methods 82 (1), 71-77 (2010)
   PUBMED   20434495
REFERENCE   2  (bases 1 to 12075)
  AUTHORS   Li,D., Liu,B., Chen,M., Guo,D., Guo,X., Liu,F., Feng,L. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2009) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P.R. China
FEATURES             Location/Qualifiers
     source          1..12075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="G1630"
                     /db_xref="taxon:562"
                     /note="serogroup: O18"
     misc_feature    1..12075
                     /note="O antigen gene cluster"
     gene            1012..2097
                     /gene="rmlB"
     CDS             1012..2097
                     /gene="rmlB"
                     /note="dTDP-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ADC54945.1"
                     /translation="MKILVTGGAGFIGSADVRHIINNTQDSVVNVDKLTYAGNLESLA
                     EISDSERYSFEHADICDAEAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSGLDDEKKKNFRFHHISTDEVYGDLPHPDEVNSNETLPLFTEMTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVFTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRH"
     gene            2097..2996
                     /gene="rmlD"
     CDS             2097..2996
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-D-glucose-3,5-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ADC54946.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQEEDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTVYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            3054..3929
                     /gene="rmlA"
     CDS             3054..3929
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ADC54947.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLHYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNYVVEMAKNLKPSARGELEITDINRIYMEQGHLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKG"
     gene            3937..4494
                     /gene="rmlC"
     CDS             3937..4494
                     /gene="rmlC"
                     /note="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ADC54948.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESYNQRVFEEAVGRKVE
                     FVQDNHSKSRKGVLRGLHYQLEPYAQAKLVRCIEGEVFDIAVDIRKSSPFFGKWVGVT
                     LSAENKRQLWIPEGFAHGFVVISDTAEFVYKTNNYYSQQAERSIIFDDKDLGIAWPLN
                     THYILSEKDLNAPTFKKISSNEYFK"
     gene            4491..5738
                     /gene="wzx"
     CDS             4491..5738
                     /gene="wzx"
                     /note="putative O antigen transporter"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ADC54949.1"
                     /translation="MSLIKNSFWNLCGYVLPAIVTLPALGIMGRKLGPELFGVFTLAL
                     AVVGYASIFDAGLTRAVIREVAIEKDNEENKLKIISSATVVIIYLSLAASLLLFFFSG
                     HIALLLNISETFFHNVSVSLKILAASIPLFLITQIWLSILEGEERFGLLNIYKSITGV
                     ILAISPALFILIKPSLMYAIIGLVLARFLCFILAFIICHDKVLKAKLTIDIPTIKRLF
                     MFGGWITVSNIISPVLSYFDRFIVSNQLGAANVAFYTAPSEIISRLSIIPGAFSRALF
                     PRLANANNSAERYKTKRLITISLLIIITPIFCIGVLFSEKIMVLWMGASFFGEPGLVL
                     SILLIGFIFNGLAQVPFASIQSRGHAKITAFVHLLELFPYLLLLFYLIKAHGVVGAGI
                     AWSVRMIVDYIALSLLDGKYINK"
     gene            5795..6985
                     /gene="wzy"
     CDS             5795..6985
                     /gene="wzy"
                     /note="O antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ADC54950.1"
                     /translation="MIYILTLTLLLVIAIMFSLLGTKSRITSPLPLHFLPWLLTLIVG
                     ISNYDQFYEFNERSFYSLLIWFTVIFIFYFIGELVNYKRENINVYYGLSHIKYECKKY
                     WIIVIPISLYTIFEIYMVGMGGADGFFLNLRLANTLEGYTGKKFILMPAVYPLMMAMF
                     AIVCLTKTSKLNKYSIYFWMFLYCIGTMGKFSILTPILTYLIIYDFKHRLKVKKTIKF
                     TLLIIILALTLHFTRMAENDHSTFLSILGLYIYSPIIALGQLNEVNSSHFGEYTFRFI
                     YAITNKIGLIKELPVNTILDYSYVPVPTNVYTALQPFYQDFGYTGIIFGAVLYGLIYV
                     SLYTAGVRGNNTQALLIYALFSVSSATAFFAETLVTNLAGNVKLVLCTILLWRFTVIC
                     KPAQ"
     gene            6967..7902
                     /gene="wekT"
     CDS             6967..7902
                     /gene="wekT"
                     /note="rhamnosyl transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WekT"
                     /protein_id="ADC54951.1"
                     /translation="MQTSTVTILMATYNGEAFIKNQILSLQQQTFSNWRLFIQDDGST
                     DNTISIIKNFQKSDSRIRLVDDNLKGQGAGKNFLSLIKYSETDYTIYCDQDDIWLENK
                     IFELVKYANEIKLNVSDAPSLVYADGYAYMDGEGTIDFSGISNNHADQLKDFLFFNGG
                     YQGCSIMFNRAMTKFLLNYRGFVYLHDDITTLAAYALGKVYFLPKYLMLYRQHTNAVT
                     GIKTFRNGLTSKFKSPVNYLLSRKHYQVKKSFFECNSSILSETNKKVFLDFISFCESN
                     NKFTDFFKLWRGGFRLNNSRTKLSLKFLIRRKFSE"
     gene            7899..8687
                     /gene="wekU"
     CDS             7899..8687
                     /gene="wekU"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WekU"
                     /protein_id="ADC54952.1"
                     /translation="MISILTPTFNRQHTLSRLFNSLILQTDKDFEWIIIDDGSIDATA
                     VLVEDFRKKCDFDLIYCYQENNGKPMALNAGVKACRGDYIFIVDSDDALTPDAIKLIK
                     ESIHDCLSEKESFSGVGFRKAYIKGGIIGNDLNNSSEHIYYLNATEISNLINGDVAYC
                     FKKESLVKNPFPRIEDEKFVPELYIWNKITDKAKIRFNISKVIYLCEYLDDGLSKNFH
                     NQLKKYPKGFKIYYKDQRKREKTYIKKTKMLIRYLQCCYYEKIK"
     gene            8684..9790
                     /gene="wekV"
     CDS             8684..9790
                     /gene="wekV"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WekV"
                     /protein_id="ADC54953.1"
                     /translation="MKILFVITGLGLGGAEKQVCLLADKLSLSGHHVKIISLGHMSNN
                     KVFPSENNVNVINVNMSKNISGVIKGCVRIRDVIANFKPDIVHSHMFHANIITRLSVI
                     GIKNRPGIISTAHNKNEGGYFRMLTYRITDCLSDCCTNVSKEAVDEFLRIKAFNPAKA
                     ITMYNGIDTNKFKFDLLARREIRDGINIKNDDILLLAAGRLTLAKDYPNLLNAMTLLP
                     EHFKLIIIGDGELRDEINMLIKKLQLSNRVSLLGVKKNIAPYFSACDIFVLSSRWEGF
                     GLVVAEAMSCERIVVGTDSGGVREVIGDDDFLVPISDSTQLASKIEKLSLSQIRDHIG
                     FRNRERILKNFSIDTIIMQWQELYGTIICSKHER"
     gene            10132..11235
                     /gene="wekW"
     CDS             10132..11235
                     /gene="wekW"
                     /note="galactosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WekW"
                     /protein_id="ADC54954.1"
                     /translation="MTARTTKVLHLQLLPLLSGVQRVTLNEISALYTDYDYTLVCSKK
                     GPLTKALLEYDVDCHCIPELTREITVKNDFKALFKLYKFIKKEKFDIVHTHSSKTGIL
                     GRVAAKLARVGKVIHTVHGFSFPAASSKKSYYLYFFMEWIAKFFTDKLIVLNVDDEYI
                     AINKLKFKRDKVFLIPNGVDTDKFSPLENKIYSSTLNLVMVGRLSKQKDPETLLLAVE
                     KLLNENVNVKLTLVGDGELKEQLESRFKRQDGRIIFHGWSDNIVNILKVNDLFILPSL
                     WEGMPLAILEALSCGLPCIVTNIPGNNSLIEDGYNGCLFEIRDCQLLSQKIMSYVGKP
                     ELIAQQSTNARSFILKNYGLVKRNNKVRQLYDN"
ORIGIN      
        1 gcggaagtgc agttcatctg tccaccgggc gtgaccatta tgaacgtgcg tcagggcgaa
       61 cctttaggtt tggggccact ccattttatg tgcacgacct gctattggtg acaaccccat
      121 ttgtcgtggt actgccagac gttgttatcg atgacgccag cgccgacccg ctgcgttaca
      181 accttgctgc catgattgct cgtttcaacg aaacgggccg cagccaggtg ctggcaaaac
      241 gtatgccggg tgacctctct gaatactccg ttattcagac caaagaacca ctggatcgtg
      301 aaggtaaagt cagccgcatt gttgaattta tcgaaaaacc ggatcagccg cagacgctgg
      361 actcagacat catggccgtg ggccgttatg tgctttctgc cgatatttgg ccggaactgg
      421 aacgtactca gcctggtgca tggggacgta ttcagctgac tgatgccatt gctgagttgg
      481 caaaaaaaca agcagttgac gcaatgctga tgactgggga cagttacgac tgcggaaaga
      541 aaatgggtta tatgcaagcg tttgtgaagt atgggctgcg taacctgaag gagggcgcta
      601 agttccgcaa aggcgttgag aagttgttaa gcgagtaaca aattcctgac tggaagtgtc
      661 ggtcagtaag taattaaaaa cggcagtgaa gattcatagc ataagttttg ctcattgaat
      721 ctcactgccg ttgtcttttt tataagtaca ataaaaacag catgttagct tgcaatcata
      781 gttcaaattt ttccaggatt ttccttgttt ctacagctgt ttggtaagac aattagcgtt
      841 ttgaattttt cggatttggc acgagtgggt tacgctcgtc acatcgtgga aatgtaagca
      901 gtgctctggt agctgctaag ccaggggcgg tagcgtgcat taatgcatct attaatcaaa
      961 tctggagcag tctatttcac agcatgctct ctggctatat ggaataaaaa agtgaagata
     1021 cttgttactg gtggcgcagg atttattggt tctgctgatg ttcgtcacat aataaataat
     1081 acgcaagata gtgttgttaa tgtcgataaa ttaacatacg ccggaaacct ggaatcgctc
     1141 gctgaaattt ctgattctga acgttattca tttgagcatg cagatatctg cgatgccgaa
     1201 gcgatggctc gtattttcgc acagcaccag ccagacgcgg tgatgcacct ggcagcagag
     1261 agccacgttg accgctcaat aactggccct gcggcattta ttgaaaccaa tattgtgggt
     1321 acttatgttc ttttagaagc ggcgcgcaat tattggtctg gtctggatga tgaaaagaaa
     1381 aaaaacttcc gctttcatca tatttctact gatgaggtgt atggtgactt accccatccg
     1441 gatgaagtaa atagcaatga aacgttgccg ctatttacgg aaatgacagc atacgcgcca
     1501 agtagtccat attctgcttc taaagcttcc agcgatcatt tggttcgcgc atggaaacgt
     1561 acttatggtt taccgaccat tgtgactaat tgctcgaaca actatggtcc ttatcatttc
     1621 ccggaaaagc ttattccact ggttattctt aatgcactgg aaggtaaggc attacctatt
     1681 tatggcaaag gagatcagat ccgcgactgg ttgtatgtag aggatcatgc tcgagcgtta
     1741 tataccgtcg taaccgaagg taaagcgggc gaaacttata acattggtgg acacaacgaa
     1801 aagaaaaaca tcgacgtagt gttcactatt tgtgatttgt tggatgagat agtcccgaaa
     1861 gagaaatctt atcgtgagca aattacctat gttgctgatc gcccagggca tgatcgccgt
     1921 tatgcaattg atgccgataa aattagccgc gaattgggct ggaaaccaca ggaaacgttt
     1981 gagagcggga ttcgtaaaac tgtggaatgg tatctgtcca atacaaaatg ggttgataat
     2041 gtgaaaagtg gtgcctatca atcgtggatt gaacagaact atgagggccg ccactaatga
     2101 atatcctcct ttttggcaaa acagggcagg ttggttggga actacagcgt gctctggcac
     2161 ctctgggtaa tttgattgct cttgatgttc actccactga ttactgtggt gattttagta
     2221 accctgaagg tgtggctgaa accgttagaa gcattcggcc tgatattatt gtcaacgcag
     2281 ccgctcacac cgcagtagac aaagcagaat cagaaccgga gtttgcacaa ttactgaacg
     2341 cgacgagtgt cgaagcgatc gcgaaagcag ccaatgaagt cggcgcttgg gttattcact
     2401 actctactga ctacgtattt ccggggaccg gtgaaatacc atggcaggag gaggatgcaa
     2461 ccgcaccgct aaatgtttac ggtgaaacca agttagcagg agaaaaagca ttacaagagc
     2521 attgtgcgaa gcaccttatt ttccggacca gctgggtcta tgcaggtaaa ggaaataact
     2581 tcgccaaaac gatgttgcgt ctggcaaaag agcgtgaaga attagccgtt attaatgatc
     2641 agtttggtgc gccaactggc gcagagttgc tggctgattg tacggcacat gccattcgtg
     2701 tggcactgaa taaaccggaa gtcgcaggtt tgtaccatct ggtagccagt ggtaccacaa
     2761 cctggcacga ttatgctgcg ctggtttttg aagaggcgcg caaagcaggc attccccttg
     2821 cactcaacaa gctcaacgca gtaccaacaa cagtctatcc tacaccagct cgtcgtccac
     2881 ataactctcg ccttaataca gaaaaatttc agcagaactt tgcgcttgtc ttgcctgact
     2941 ggcaggttgg tgtgaaacgc atgctcaacg aattatttac gactacagca atttaatagt
     3001 ttttgcatct tgttcgtgat ggtggaacaa gatgaattaa aaggaatgat ggaatgaaaa
     3061 cgcgtaaagg tattatttta gcgggtggtt ctggtacacg tctttatcct gtgactatgg
     3121 ctgtcagtaa acagctgtta ccgatttatg ataaaccgat gatctattac ccgctctcta
     3181 cactgatgtt ggcgggtatt cgcgatattt tgattatcag cacgccacag gatactcctc
     3241 gttttcaaca actgctgggt gatgggagcc agtgggggct aaatcttcac tacaaagtgc
     3301 aaccgagtcc ggatggtctt gcgcaggcat ttatcatcgg tgaagagttt atcggtggtg
     3361 atgattgtgc tttggtactt ggtgataata tcttctacgg tcacgacctg cctaagttaa
     3421 tggatgccgc tgttaacaaa gaaagtggtg caacggtatt tgcctatcac gttaatgatc
     3481 ctgaacgcta tggtgtcgtt gagtttgata aaaacggtac ggcaattagc ctcgaagaaa
     3541 aaccgctaca accaaaaagt aattatgcag taaccgggct ttatttctat gataactacg
     3601 ttgtggaaat ggcgaaaaat cttaagcctt ctgcccgcgg tgaactggaa attaccgata
     3661 ttaaccgtat ttatatggaa caggggcatt tatctgttgc catgatggga cgtggttatg
     3721 catggctgga cacggggaca catcagagtc ttattgaagc aagcaacttc attgccacca
     3781 ttgaagagcg ccagggacta aaggtttcct gcccagaaga aattgcttat cgtaaaggat
     3841 ttattgacgc agagcaggtt aaggtattag ccgagccgct gaagaaaaac gcttatggtc
     3901 agtatttgct gaaaatgatt aaaggttagt aataaaatga atgttattaa aacagaaatt
     3961 ccagacgtac tgatttttga accgaaagtt tttggtgacg agcgcggttt ctttttcgaa
     4021 agctataacc agagggtttt tgaggaagct gtaggtcgca aagttgagtt tgttcaggat
     4081 aaccattcta aatcgagaaa aggagtattg cggggattgc attatcaatt agagccgtat
     4141 gcgcaagcaa aacttgtgcg ttgcattgag ggtgaagtat ttgatattgc tgtagatata
     4201 cggaagtcat ctccattttt tggtaaatgg gttggtgtaa cattatccgc tgaaaataaa
     4261 cgtcaattat ggatccctga agggtttgct catggttttg tggtgattag tgatactgcg
     4321 gaatttgtct ataaaacgaa caattattac agtcaacaag cagagcgaag cataattttt
     4381 gatgataaag acttagggat tgcttggcca ttgaatactc attatattct ttcagaaaaa
     4441 gatttaaatg cgccaacatt taagaaaata tcgagtaatg agtattttaa atgagtttaa
     4501 tcaaaaacag tttttggaac ctttgcgggt atgtacttcc agctattgtg acactaccag
     4561 ctttgggtat tatggggcga aaattaggcc cagaattatt tggtgtattc actttggcat
     4621 tagctgttgt gggttatgca agcatttttg atgcaggcct tactcgcgca gtgatacgag
     4681 aagtcgcaat tgaaaaagat aatgaagaaa ataagttgaa aattatttct tcagcgacag
     4741 ttgtaattat ttatttgagt ttggccgcct cactcttatt attttttttt agtggtcata
     4801 tcgcattgct actgaacatt agtgagactt tttttcataa tgtaagtgtc tcgcttaaaa
     4861 ttctcgcagc atccatacca ttatttttga ttactcaaat atggttgtca attttagaag
     4921 gtgaagaaag atttggttta cttaatatct acaaatcaat tacgggagtg atattagcaa
     4981 tctcaccggc attatttata cttattaaac cctctttgat gtatgcgata ataggcttag
     5041 ttctagcaag gtttttatgt tttattttgg cttttataat ttgtcacgat aaagtgctta
     5101 aagctaaact aacaatcgat ataccaacaa ttaaaagatt gtttatgttc ggtggttgga
     5161 ttacagttag taatatcatc agccctgtgc tatcatattt tgataggttt attgtttcaa
     5221 atcaacttgg ggctgctaat gttgcttttt atactgcacc atcagaaatt atttctcggc
     5281 ttagtataat tccaggtgcg ttttcaagag ccttattccc aagattagct aatgcaaata
     5341 attccgctga aagatataaa acgaaaagat taattacaat ttcactttta ataatcatca
     5401 cccctatttt ttgtattggc gtgttatttt cagagaagat aatggtttta tggatggggg
     5461 catcattttt tggtgagcct ggtttggtat tatcaatatt actgattggc tttattttta
     5521 atggattggc acaagtacca tttgccagta ttcaatcccg aggtcatgct aagataactg
     5581 catttgttca tctcttagag ttgtttcctt atttattact tttattttac ctcataaaag
     5641 cacatggggt tgttggcgcg ggtattgcgt ggtcagtgag gatgatagta gattatatag
     5701 cattaagtct tttggacggt aagtatatta ataaataaaa ttcaaaatgc aagttaataa
     5761 ctcatggctt tatttgggta ggtgacaatt tataatgata tatatattaa ctttaactct
     5821 tcttctagtt atagccataa tgttttctct tctcggcaca aaaagtagga tcacatctcc
     5881 attacctttg cattttttac catggttact aactttaatt gtcgggataa gtaattacga
     5941 tcaattttac gagtttaatg aaagaagctt ttactctttg ttgatttggt ttacagttat
     6001 ttttatattt tatttcatag gggaactggt taattataaa cgtgaaaata taaatgttta
     6061 ttatggtctt tcacatatta aatatgaatg taaaaaatat tggatcattg tcatcccaat
     6121 ttcattatat accattttcg aaatatatat ggttggtatg gggggagcag atggattctt
     6181 tctcaattta cgtcttgcaa atacattgga gggctatacg ggtaaaaaat ttatcttaat
     6241 gcctgctgta tatcctctaa tgatggctat gttcgcaatt gtttgtctaa caaaaacttc
     6301 caaattaaat aaatactcca tttatttctg gatgtttttg tattgtattg gcacaatggg
     6361 aaaattttca atattaacgc caatattgac atatttaatt atttatgact tcaaacatag
     6421 attaaaagta aaaaaaacaa taaagtttac attgttgata attatattag ctttaacttt
     6481 gcattttaca cgtatggctg agaatgacca ctcaacattt ttatctattt tagggctcta
     6541 tatttattca ccaataattg ctttaggcca gttgaatgaa gtaaatagta gtcattttgg
     6601 tgagtatacg tttagattca tatatgctat aactaataaa attggcctta ttaaagaatt
     6661 gccagtaaat actattcttg actattcata cgttcctgta ccaacaaatg tatatactgc
     6721 acttcaacca ttttaccagg attttggtta tactggcatc atatttggag cagtattata
     6781 cggactaata tatgtgagtt tatacacggc cggtgttcgt ggaaataata cacaggcatt
     6841 actgatttac gcattgtttt cagttagcag tgcaacggct ttcttcgctg aaacgctagt
     6901 aacgaattta gctggaaatg tgaagttagt attatgtacc atcttactat ggcgatttac
     6961 agtaatatgc aaaccagcac agtaaccatt ctaatggcca cctacaatgg cgaggccttc
     7021 atcaaaaatc agattttgtc actacaacaa caaacatttt ctaactggcg gttatttatt
     7081 caggatgatg ggtctacaga caatactata tctataataa aaaacttcca aaaatctgac
     7141 tccagaattc ggctagttga tgataatttg aaaggtcaag gtgcaggaaa aaatttttta
     7201 tcgctgataa agtacagcga gacagattat acaatttatt gtgaccaaga tgatatttgg
     7261 ttagaaaaca aaatatttga attagtaaag tatgcaaatg aaattaaatt gaatgtatca
     7321 gatgcgcctt cgctagttta tgctgatggc tatgcttata tggatggtga gggtacaatc
     7381 gatttttctg ggatatctaa caatcatgct gatcaattaa aggattttct tttttttaat
     7441 ggtggatacc aaggatgttc tattatgttc aatcgtgcaa tgaccaaatt tcttctgaat
     7501 tatcgaggat ttgtatatct acatgacgat atcacaacat tagctgcata cgctcttggt
     7561 aaagtttatt ttctcccgaa ataccttatg ttatatagac agcacacgaa tgcggtaact
     7621 ggtatcaaaa cattccgcaa tggattgact tctaaattta aatcaccagt aaactatctt
     7681 ttatcacgaa aacattatca ggtaaaaaaa tctttttttg aatgtaacag ctctatctta
     7741 tcagagacga ataaaaaagt ttttttggat tttatttcat tttgtgaatc aaataataaa
     7801 tttacagatt tttttaagtt atggcgaggt gggtttagat taaataacag tagaactaaa
     7861 ttatcattaa aattcttaat acggagaaaa tttagcgaat gatttcaata cttacaccta
     7921 cttttaatcg gcaacatact ttatcaaggc tattcaattc tcttatatta caaactgata
     7981 aagattttga gtggataata attgatgatg gtagtataga tgcaacagcg gtacttgtag
     8041 aagattttag aaaaaaatgt gattttgact tgatttattg ctatcaggaa aataatggta
     8101 agcccatggc tttaaacgct ggtgttaaag cttgtagagg cgattatatc tttattgttg
     8161 acagtgatga tgcactaact cccgatgcca taaaattaat taaagaatca atacatgatt
     8221 gcttatctga gaaggaaagt ttcagcggag tcggttttag aaaagcatat ataaaagggg
     8281 ggattattgg taatgattta aataattctt cagaacatat atactattta aatgcgactg
     8341 agattagcaa tttaataaat ggtgatgttg catattgttt taaaaaagaa agtttggtaa
     8401 aaaatccatt cccccgtata gaagatgaaa aatttgttcc agaattatat atttggaata
     8461 aaataactga caaggcgaag attcgattta acataagcaa agttatatat ctttgtgagt
     8521 atcttgatga tggtctttct aaaaatttcc ataaccagct taaaaaatac ccaaaggggt
     8581 ttaagattta ttacaaagat caaagaaaac gagagaaaac ttatataaaa aaaacaaaga
     8641 tgctaattag atatttgcaa tgttgttatt atgagaaaat aaaatgaaaa tactatttgt
     8701 cattacaggt ttaggccttg gaggtgctga gaagcaggtt tgtcttttag ctgataaatt
     8761 aagtttaagc gggcaccatg taaagattat ttcacttgga catatgtcta ataataaagt
     8821 ctttcctagc gaaaataatg ttaatgtcat taatgtaaat atgtcaaaaa acatttctgg
     8881 agttataaaa ggttgtgtca gaattagaga tgttatagct aatttcaaac cagacattgt
     8941 acacagtcat atgtttcatg caaacattat cactagattg tctgtaattg gaatcaaaaa
     9001 cagacctggt attatatcaa ctgcacataa taaaaatgaa ggtgggtatt tcagaatgct
     9061 cacatataga ataaccgatt gtttaagtga ttgttgtaca aatgttagca aagaagcagt
     9121 ggatgagttt ttacggataa aagcctttaa tcccgctaaa gcaattacta tgtataatgg
     9181 gatagatacc aataaattta aatttgattt attggcaagg agggaaattc gagacggtat
     9241 taatataaaa aatgatgata tattattact tgctgcaggt cgtttaacgt tagctaaaga
     9301 ttatcctaat ttattgaatg caatgactct gcttcctgaa cactttaaac ttattattat
     9361 tggtgatggt gaattgcgtg acgaaattaa tatgcttata aaaaaattgc aattatctaa
     9421 tagggtgtcc ttgttgggag ttaaaaaaaa tattgctccc tatttttctg catgtgatat
     9481 ttttgttctc tcttctcgtt gggaaggatt tggattagtc gtggcagaag ctatgtcatg
     9541 tgagcgaatt gttgttggca cggattcagg gggagtaaga gaagttattg gtgacgatga
     9601 ttttcttgta cccatatctg attcaacaca acttgcaagc aaaattgaaa aattgtcttt
     9661 gagccagata cgtgatcaca ttggttttcg gaatcgtgag cgtattttaa aaaatttctc
     9721 aatagatact attattatgc agtggcaaga actctatgga actataattt gctcaaaaca
     9781 tgaaaggtag atttatattt ggaacgtgtc ttttgtttga atttaattca atctcaattg
     9841 agatttttgt atttcaaaaa taccatcata gctaacgatg attggtattt attttaagat
     9901 gctttctata aatatattga cgtttttaat gcgccgaaac gattgggctg ggaacagaga
     9961 agtaaaactg ttttgagaat gaagagtttt tgagatgttt atggatatta aaaattgatc
    10021 cagtgaatta attatttata ataaatcaag atttaatgtt aataaatgat aatcttttct
    10081 gacactcata ttaattatga gtggtacgtt tggtaaacgg taaactatta tatgacagct
    10141 agaacaacta aagttttgca cttacaatta ctcccactct taagtggcgt tcaaagggta
    10201 acattaaacg aaattagtgc gttatatact gattatgatt atacactagt ttgctcaaaa
    10261 aaaggtccac taacaaaagc attgctggaa tatgatgtcg attgtcattg tatccccgaa
    10321 cttacgagag aaattaccgt aaagaatgat tttaaagcat tgttcaagct ttataagttc
    10381 ataaaaaaag aaaaatttga cattgtgcat acacattctt caaaaacagg tattttgggg
    10441 cgagttgctg ccaaattagc acgtgttgga aaggtgatcc acactgtaca tggtttttct
    10501 tttccagccg catctagtaa aaaaagttat tacctttatt ttttcatgga atggatagca
    10561 aagttcttta cggataagtt aatcgtcttg aatgtagatg atgaatatat agcaataaac
    10621 aaattaaaat tcaagcggga taaagttttt ttaattccta atggagtaga cactgataag
    10681 ttttctcctt tagaaaataa aatttatagt agcaccttga atctagtaat ggttggtaga
    10741 ttatccaagc aaaaagatcc tgagacatta ttgcttgctg ttgaaaaact gctgaatgaa
    10801 aatgttaatg ttaagctgac acttgtagga gatggtgaac taaaagaaca gttagaaagc
    10861 aggttcaaac ggcaagatgg acgtataatt tttcatggat ggtcagataa cattgttaat
    10921 attttaaaag ttaatgatct ttttatatta ccttctcttt gggagggtat gccattagca
    10981 attttagaag cattgagctg tggacttcca tgtatagtca ctaatattcc aggtaataat
    11041 agcttaatag aagatggcta taatggttgt ttgtttgaaa ttagagattg tcagttatta
    11101 tctcaaaaaa tcatgtcata tgttggtaag ccagaactga ttgcacagca atctaccaat
    11161 gcacgatcat ttattctgaa aaattatgga ttagttaaaa gaaataataa ggtcagacag
    11221 ctatatgata attaaatgaa accgaaaagt taaaaaagaa caggtttttc aaagtgaaaa
    11281 taaaattaca gtttttttat tgcaatgatt aacgtaacat ctgcattaca ttcaagccgc
    11341 acaaccccgc ggtgaccacc cctgacagga gtaaacaatg tcaaagcaac agatcggcgt
    11401 cgtcggtatg gcagtgatgg gacgcaacct cgcgctcaac atcgaaagcc gtggttatac
    11461 cgtctctatt ttcaaccgtt cccgtgaaaa gacggaagaa gttattgccg aaaatccagg
    11521 caagaaactg gttccttact atacggtgaa agagttcgtt gaatctcttg aaacgcctcg
    11581 tcgcatcctg ttaatggtta aagcaggtgc aggcacggat gctgctattg attccctgaa
    11641 accatatctc gataaaggcg atatcatcat tgatggtggt aataccttct tccaggacac
    11701 cattcgtcgt aaccgcgagc tttctgcaca aggctttaac ttcatcggta cgggtgtttc
    11761 cggtggtgaa gagggcgcgc tgaaaggacc ttctatcatg cctggtgggc agaaagaggc
    11821 ctatgaactg gttgctccta tcctgaccaa aatcgccgcc gtggctgaag atggtgaacc
    11881 atgcgttacc tatattggtg ccgatggcgc aggtcactat gtgaagatgg ttcacaacgg
    11941 tattgaatac ggtgatatgc aactgattgc tgaagcctat tctctgctga aaggtggtct
    12001 gaatctctct aacgaagaac tggcacaaac ctttaccgag tggaataacg gtgaactgag
    12061 cagttacctg atcga
//

</pre>