LOCUS       KJ739600               15029 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  UNVERIFIED: Escherichia coli strain SSI 81829 O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ739600
VERSION     KJ739600.2
KEYWORDS    UNVERIFIED.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15029)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15029)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Baranzoni,G.M.,
            Swimley,M., Burrell,A.M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
REFERENCE   3  (bases 1 to 15029)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Baranzoni,G.M.,
            Swimley,M., Burrell,A.M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
  REMARK    Sequence update by submitter
COMMENT     GenBank staff is unable to verify sequence and/or annotation
            provided by the submitter.
            On Dec 18, 2014 this sequence version replaced KJ739600.1.
            
            ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: 90X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SSI 81829"
                     /serotype="O187:K-:H52"
                     /db_xref="taxon:562"
     misc_feature    1..15029
                     /note="O-antigen gene cluster"
     gene            128..1204
                     /gene="rmlB"
     CDS             128..1204
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="AIG56956.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTA
                     YAPSSPYSASKASSDHLARAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTINWY
                     LNNNDWCRRVQDGSYQRERLGVIR"
     gene            1201..2064
                     /gene="rmlA"
     CDS             1201..2064
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="AIG56955.1"
                     /translation="MKGIILAGGSGTRLYPITMGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIQDILIITTPEDQQGFIRLLGNGSQFGINLNYATQRNPDGLAQAFIIGEQFIGEDSV
                     CLVLGDNIFFGQGFTPKLRCAAEREIGATIFGYQVMDPERFGVVEFDCNYKALSIVEK
                     PNKPKSNWAVTGLYFYDNNVIDIAKKIKPSHRGELEITSVNEVYLKNNELTVELLGRG
                     FAWLDTGTHDSLIEASNFVETVQRRQGMMVACPEEIAWRNGWLSNEDLYKLGTKIKKN
                     LYGKYLIDLVK"
     gene            2066..2470
                     /gene="fdtA"
     CDS             2066..2470
                     /gene="fdtA"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-3,4-keto-hexulose isomerase"
                     /protein_id="AIG56954.1"
                     /translation="MNVEFIPLQKHGDERGMLVALEQAKNIPFEIKRVYYMFGTQGNV
                     RRGYHAHKKIRQVAIPLNGSCRFHLDNGREKIDVVLDDPALGLLIEPGVWHEMYDYSK
                     NCILLVLASDIYDENDYIRNYEDFMRSVKNDS"
     gene            2460..2918
                     /gene="fdtC"
     CDS             2460..2918
                     /gene="fdtC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose
                     3-N-acetyltransferase"
                     /protein_id="AIG56953.1"
                     /translation="MIHKLSDVQTIHIGKKTRVWQYSVILPGAVIGDECNICAHTLIE
                     NDVVIGDRVTIKSGVYIWDGVFIEDDVFIGPCVTFTNDKIPRSKKYPEKFLKTIIKKG
                     ASIGANATILPGITIGENAMVGAGTVVTKDVAPYTVIAGNPGKVIRDIKE"
     gene            2921..4024
                     /gene="fdtB"
     CDS             2921..4024
                     /gene="fdtB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose
                     transaminase"
                     /protein_id="AIG56952.1"
                     /translation="MIDFLNLKRINEKYKAEMQDAFNRVVDSGWYIMGKELKSFESNF
                     AQYCGVKYAIGVANGLDALYLVLKAWKEQGKLRDGDEVIVQANTYIASILAITNNNLT
                     PILIEPDENSYNLNPELIRSKITAQTRAILVVHLYGQMSPMKAIMEIANEYNLLVLED
                     CAQAHGAQLDNIKAGNWGHAAGFSFYPGKNLGALGDAGAITTNDDELASVINAIRNYG
                     SHKKYHNVYQGVNSRLDELQAALLNVKLKYLDEDNSARQKIASRYISEIKNKHITLPV
                     SKNVEEHVWHLFVIKTEKREELVTYLNNNDIMTMIHYPIPPHKQVAYKEFNGLSFPLT
                     EQLHDKVLSLPIDPTMTDCDIEYVIKVVNGFVG"
     gene            4021..5286
                     /gene="wzx"
     CDS             4021..5286
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AIG56951.1"
                     /translation="MNKLLSVTFLTGLLTFARMVAGFIVAKIVAIYTGPTGIAMLGQL
                     QNVINGVSGLVNSPLNTSIVRFTAENYRTGYEECAIWWRVSIRWAIIIYAVIAPMMVV
                     LSKQLSSFLFESDLYYWVIIASALALPFTAVGTLINSIINGQQLYIQYVLLGMVSVII
                     SSMIMISLVVIKGVEGALIALALQNAIIGIAMFGYGLRRPWFKLQYFFGNISKEQSKI
                     ILGYTFMALVSALTMPVVLMLIRKFLVMEVGWEHTGYWQAVWKISETYLAVVTMSLSI
                     YYLPQLSSINDITSLRKEIYKTVKVVMPIVILMAFAIYMLRDIAISLLFTETFKGARD
                     LFLVQLCGDVVKILSWLFAYALISRGETKLFIASEAISAITFILLAFFCIKFYGVQGA
                     NIAYLANYVIYFILIYYCVFYRLKSHRCQ"
     gene            5296..6237
                     /gene="epsE"
     CDS             5296..6237
                     /gene="epsE"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase 2 family protein"
                     /protein_id="AIG56950.1"
                     /translation="MELLEKIITVCVVTYNSFTTVIKTLESIKKQTYDTNFIELIIAD
                     DCSSDDTVIIVKKWIEENKCIFNNVVLIEQKQNIGLTKNINSAWKLAKGSWVKSIAGD
                     DILLENCIANNITYVNKHNIQSVVFSLMRSFTEDNNKITFHEIYPNSYQQKVLKGERD
                     EQIKYLTHAGGFACAPSAFINRRLLEEVGFADERFFMLEDTPLWFRILKKGEKIYFMN
                     IFTVKYRIGNSISQSNKSLFNVRHLSQKMEVDFLLFKNEVGLIANIRKFIFYFICFFL
                     VFTTGANKNFISKTVFRLALLVKPFWLREKIKNAKII"
     gene            6221..7498
                     /gene="wzy"
     CDS             6221..7498
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AIG56949.1"
                     /translation="MLKLYKNKAQEYYLLICLFLCVFNFYVPSVGPALYISIAMVFVY
                     LVVNIKSLCKYNSVISRAYVSYIFIYGLPFLFLICFVGVRTILSGSGDTSFLFLLIKY
                     FVFGCVASLFAVAIFISSEITSLSDVQALFYRITLVQSLIIIAAVLSPSISEFVRLYQ
                     NNDANNSSLVLEGLRGVALSSMQFYSLACYFCIITILLADDFINNKIGYLKTSVFLLL
                     MAISSIFISRTAIIGVLLFFVYTLNPFIKYGKKRSVAMALMFLVLTLVFLLFSGLIFT
                     EQVSIIQQKVLPWAFELIYRYSETGSFSTASTDELKSMYFSLNEITMLIGDGKYTGDT
                     PGTYYMGTDAGYMRPTLFGGFFLILAMLVIWILWLKNTFYPKKGGVLFISLVILSLIL
                     QYKGEFIITNYCTLILLSTLLMVSQLLKQKFRG"
     gene            7502..8605
                     /gene="pglH"
     CDS             7502..8605
                     /gene="pglH"
                     /codon_start=1
                     /transl_table=11
                     /product="galNAc-alpha-(1->Feature
                     gb|4)-GalNAc-alpha-(1->Feature
                     gb|3)-diNAcBac-PP-undecaprenol
                     alpha-1,4-N-acetyl-D-galactosaminyltransferase"
                     /protein_id="AIG56948.1"
                     /translation="MAVKKKICFFAIDLYERGGLQRVTIDIANQLSNYCEVYILTFQN
                     EGFNSFAYRLDKRVQVTYLKRMCKTRTLKRKAQSILQMCHIRRYLKLHNFDSFISVGM
                     ASVVWTCIPQLMLRCRYICWDHTSFLRTDTWAVRGRRLSRLFADKIVVLTSKDAALWK
                     SSKVSVIYNPSPFDIRRADCEISRYRKKNQIVSLGRFVPVKGFDRLLDIWKIVNSKCS
                     DPFSLMLIGEGPLKEHLTERIVNENIPNISIEPFTENINIVYCESKIQLLTSTFEGLS
                     MVLIEGMTYCLPAISFDIPSGPGEIVDDGITGYLVEDNENEVFADKIILLMSNDSLLF
                     KLSDNCKKKRNIFDASEIIDHWLSLLNIKCGKA"
     gene            8602..9681
                     /gene="epsD"
     CDS             8602..9681
                     /gene="epsD"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="AIG56947.1"
                     /translation="MNILFLSNASGYGGSERTLEILAKHMAKKNKVTVFVQNEIHRDK
                     LNESGICVIEADKGWIVKKIIKDLYLIYDEIMRNDGVIANTNKAAMYLAILSFFLPKR
                     KIKKSIVFVRDFQWKYCHFIFWALSNSIICVASPAVKEYVNKYKVRPLVIPNPIEFKY
                     KALSLDVGKEDMSTPIILCPAMISRWKGLEYLIRSLALIETDCQLYIIGKIVDEEYYS
                     FIKDEVVLLKLSERVRFVDFTSDISNYYKMSTVIVNTSISDYGGPETFGRTIIEGWSF
                     KKPVVSFDCGGPKYLIQNNVDGVLVKEKSVFDLACALQKILHDRNYSNMLGMNGYNKA
                     LQKYNVDIITKTILKELYETAVRNK"
     gene            9678..10829
                     /gene="mshA"
     CDS             9678..10829
                     /gene="mshA"
                     /codon_start=1
                     /transl_table=11
                     /product="D-inositol 3-phosphate glycosyltransferase"
                     /protein_id="AIG56946.1"
                     /translation="MIFACRQISFLTIIQYGERMYIIIDARMINASGIGTYIKNLLRE
                     INKYDSIRLQCIINHEHIEEVKSIGINDYIIFKAGFFSPFEQLEYIVKLPSADIFWTP
                     HFNTSIFSLKDKFKKRVVTIHDMFPFAYRDSYKKSHFFYIKFLMKKAAKISDLILTVS
                     NFSKVQIIDYLDVSEEKVLVVPLGVDPDFLNTSNSSSVKKKYILCVGNVKPHKNIKRA
                     IKAFIAVADEIPSYELVIVGKSEGFYSPEDNLKELIQGNKRVHFTGFVSIEELKKYYS
                     EASMLLFPSLYEGFGLPLLEAMSFGLPIAASARASLPEVGKNAIIYFDPESVDDIGNA
                     IVGIINGDVKCNTLAYQSILNEYTWEKCTSETLNSFRKLVLENESSSNR"
     gene            10807..11922
                     /gene="kanF"
     CDS             10807..11922
                     /gene="kanF"
                     /codon_start=1
                     /transl_table=11
                     /product="2-deoxystreptamine glucosyltransferase"
                     /protein_id="AIG56945.1"
                     /translation="MKVLQIGKFFYPVRGGVETVAYDIAELLNSKKIKCDVLCTNKNQ
                     TTTIELHNGYKVYRSGIIGSVFSTQISLQYIKTLKNIIDDYDILHVHLPNPLANIAII
                     LANTKDKKIVLHWHSDIIKQKYLLKLYRPFQNWLLKKADAIIGTSQNYIDNSSDLLPF
                     RYKCVPIPIGIDPHRLLAEHHKVKNIKEKFSGKKIILSLGRHVYYKGFNYLIEAAQYL
                     SDDYVILIGGDGPLTSVMHDRIKTLNLTNRVFLIGSIPGNDLGSYFEACDVFCLPSIM
                     KSEAFGIVQIEAMSFGKPIVATNIQGSGTSWVNENKVSGLNVDIKNPEQIASAIYNIT
                     CESVRYREYCNSSFERFNQFFHRNVMIDHIIKLYQTL"
     gene            11944..13341
                     /gene="manC"
     CDS             11944..13341
                     /gene="manC"
                     /EC_number="2.7.7.13"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="AIG56944.1"
                     /translation="MIVPIIIAGGTGSRLWPLSRSLYPKQFLPLVNESTMIQDTIDRL
                     KYIEHLDPIIICNEEHRFIVAEQLREKEYKHNGIILEPVGKNTAPAVALGAHRALEIT
                     NDPILLVLAADHSILNIHNFSEAVNLANEAAISGKLVTFGTVPSAPETGYGYIKKGGL
                     ISNGIYQVEKFVEKPDRITAQKYIESHNYLWNSGMFMFHAKSFLSELCKFNSKMNSAC
                     KQTYTNAKKDLEFIRLPHDDFIKCPEDSVDYAVMEHTSNAVVIPLDAEWNDVGSWSSL
                     WDISAKDIQGNGTKGDVITINTQNSYIHASDRLVTSVGLNDVIIVETKDAILVANKNN
                     VQDVKELVNRLKSLNRKEYIEHSQCYRPWGQHESIAHGPRFHVKHVIVKPGEKITEQV
                     HFHRTEHWIVVSGTAKVTKNEVSFIMKENESTFIPAGTVHTLENPGKIQLEVIEIQSG
                     NYLDDDDILRFDVKK"
     gene            13355..14791
                     /gene="manB"
     CDS             13355..14791
                     /gene="manB"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="AIG56943.1"
                     /translation="MNNYLLKSKALIVNCNVVFGTSGARGLVIDFSHDVCAAFTHAFL
                     SVIDDKYNFNKVALAIDNRPSSYEIAQACAYAIKQHGFEVEYHGVIPTPALAHYSMQK
                     NIPCIMVTGSHIPFDRNGLKFYRPDGEITKEDELAIINSEYTFSPVGVLPHLETSTQG
                     ADYYLERYVSLFNPEILKGKRIGVYEHSSAGRDLYAPLFNQLGAEVISLGRSDEFVPI
                     DTEAVSDEDRILAREWSKKYNLDAIFSTDGDGDRPLVADENGEWLRGDILGLLTAIEL
                     NIKALAIPVSCNTAIEESNKFASIQRTKIGSPYVIAAFADLAKQFDSVAGFEANGGFL
                     LASDLQINGKELKSLPTRDAVLPALMLLIASRNSTISQLINNLPQRFTWSDRVKNFPS
                     DSSQQIIKNAISSPNNFFNNLGYESLSCSAIDETDGARFTLNNGDIIHLRPSGNAPEL
                     RCYAEASDENQAKQYVANVLGNITSLIS"
ORIGIN      
        1 gtaggcatgc atgcagtgct ctggtagctg taaagccagg ggcggtagcg tgcattaata
       61 cctctattaa taaaactgag agccgcttat ttcacagcat gctctgaagt aatatggaat
      121 aaattaagtg aaaatacttg ttactggtgg cgcaggattt attggttctg ctgtagttcg
      181 tcacattata aataatactc aggatagtgt tgttaatgtc gataaattaa cgtacgccgg
      241 aaacctggaa tcacttgctg atgtttctga ctctgaacgc tatgtttttg aacatgcgga
      301 tatttgcgat gctgctgcaa tggcgcggat ttttgctcag catcagccgg atgcagtgat
      361 gcacttggct gctgaaagcc atgtggatcg ttcaattaca ggccctgcgg catttattga
      421 aaccaatatt gttggtactt atgtcctttt ggaagcggct cgcaattact ggtctgctct
      481 tgatggcgac aagaaaaata gcttccgttt tcatcatatt tctactgacg aagtctatgg
      541 tgatttgcct catcctgacg aagtaaataa taaagaacaa ttacccctct ttactgagac
      601 gacagcttac gcgcctagta gtccttattc cgcatcaaaa gcatccagcg atcatttagc
      661 ccgtgcgtgg aaacgtacct atggtttacc gaccattgtg actaactgtt cgaataacta
      721 tggtccttat cactttccgg aaaaattgat tccactagta attcttaatg ctctggaagg
      781 taaggcatta cctatttatg gcaaagggga tcaaattcgt gactggctgt atgttgaaga
      841 tcatgcgcgt gcgttatata ccgtcgtaac cgaaggtaaa gcgggtgaaa cttataacat
      901 tggtggacac aacgaaaaga aaaacatcga tgtagtgctc actatttgtg atttgttgga
      961 tgagattgta ccgaaagaga aatcttaccg cgagcaaatt acttatgttg ccgatcgccc
     1021 gggacacgat cgccgttatg cgattgatgc agagaagatt agccgcgaat tgggctggaa
     1081 accgcaggaa acgtttgaga gcgggattcg taaaacaatt aactggtatt tgaataataa
     1141 tgattggtgc cggcgagttc aagatggctc ttatcaacgt gagcgtttgg gggtaattag
     1201 atgaaaggaa ttattttagc tggtggttct ggaacaaggc tttatccaat tacaatggga
     1261 gtttcaaagc agctattacc catttatgat aaaccgatga tttattatcc tttgtcagtg
     1321 ctgatgttgg caggtataca agatattctt attattacaa cgccagaaga tcagcagggg
     1381 ttcataagat tacttgggaa cggaagccaa tttggtatta atcttaatta cgccactcaa
     1441 cggaatcctg atggtttagc tcaggccttc atcatcgggg aacaatttat tggtgaggat
     1501 tctgtctgct tagttttggg agataatatt ttttttggtc aaggatttac tccgaaactt
     1561 agatgcgccg ctgagaggga aataggagcc acgatatttg gttatcaggt aatggatcct
     1621 gaacgattcg gtgtggtcga atttgattgc aattataaag cattaagtat tgttgaaaaa
     1681 ccaaataaac caaaatcaaa ctgggctgta actggattgt atttttacga taacaatgtt
     1741 attgatattg caaaaaaaat aaaaccgtcg catcgtggtg aattagaaat cacatctgtt
     1801 aatgaggttt acttaaaaaa taatgagtta actgttgagt tattaggtcg agggtttgcg
     1861 tggctggata ccggcacaca tgatagtctc attgaagcat ctaattttgt cgaaaccgtt
     1921 caacgtagac aaggaatgat ggttgcatgc cccgaagaaa tagcttggcg gaatggttgg
     1981 ttatctaatg aagatttata taaattaggc acgaaaatta aaaagaatct atacggaaaa
     2041 tatcttatcg atttggtgaa ataatatgaa tgtcgagttt attccattac aaaaacatgg
     2101 tgatgaaaga gggatgttag tagccctaga gcaagccaaa aacattccat ttgaaattaa
     2161 acgtgtttat tatatgtttg gaactcaagg aaatgttcgg cgcgggtatc atgcccataa
     2221 aaaaattagg caagtagcga taccgctaaa tggttcatgc cgttttcatt tagataatgg
     2281 tcgtgagaaa atagatgttg ttttagatga tcctgcatta gggttgctta tagagcctgg
     2341 agtctggcat gaaatgtacg attactcaaa gaactgtatt cttctagtat tggcgagcga
     2401 tatttatgat gaaaatgact atattagaaa ttatgaggat tttatgaggt ctgttaaaaa
     2461 tgattcataa attaagcgat gttcaaacta tccatatagg taaaaaaaca agggtctggc
     2521 aatattcagt tatattaccg ggtgctgtaa ttggtgatga gtgtaacata tgtgcacata
     2581 cacttattga aaatgatgtt gtaattgggg atagagttac tattaaatcg ggcgtatata
     2641 tttgggatgg ggtttttatt gaagatgatg tatttatagg gccctgtgta acatttacca
     2701 atgataaaat ccccaggtca aagaaatacc cagaaaaatt tcttaagact attattaaaa
     2761 aaggtgcttc cattggagcc aatgcaacta tattacctgg aataacaatt ggtgaaaatg
     2821 ctatggttgg ggctggtacc gtagtcacta aagatgttgc accttataca gtcattgcag
     2881 gtaatccagg taaagttatt agggatatta aggaataaaa atgatcgact ttctcaactt
     2941 aaaaagaatc aatgaaaaat ataaagcgga aatgcaagat gcctttaatc gagttgtcga
     3001 ttctggctgg tatattatgg ggaaagaatt aaagtctttt gagagtaatt ttgcgcagta
     3061 ctgcggggtg aaatatgcaa taggtgtagc taatggatta gatgctctct atctcgtatt
     3121 aaaagcgtgg aaagaacagg ggaaattgag agatggtgat gaagtgatag ttcaagcaaa
     3181 tacgtacatt gcttccattc tcgctataac caacaataat cttacaccta tattaataga
     3241 acctgatgaa aatagttata atttaaaccc tgaattaatt cgctctaaaa taacggcgca
     3301 aacaagagcg atattggtag ttcatctata tggccaaatg tcaccgatga aagcgataat
     3361 ggaaatcgct aatgaatata atttgttagt attagaggat tgcgcacaag cccatggtgc
     3421 tcaattagat aatattaaag ctggtaactg ggggcatgct gcaggtttca gtttttatcc
     3481 cgggaaaaat ttaggcgcct taggggatgc gggagcaatt acaacaaatg atgacgaatt
     3541 agcgtcagta ataaatgcaa ttagaaatta tggttctcat aaaaaatacc ataatgttta
     3601 tcaaggtgtt aatagtcgtc tcgatgaact gcaggcagca ttattaaatg tgaaactaaa
     3661 atatcttgat gaagacaatt ccgctcgaca gaaaatagct tctagatata tttcagaaat
     3721 aaaaaataaa catatcacgt tgcctgtttc taagaatgtt gaagaacatg tctggcatct
     3781 ttttgttata aaaaccgaaa aaagagaaga gttggttaca tatttgaata ataatgatat
     3841 catgactatg attcactatc ctattccacc gcataaacaa gttgcatata aagagttcaa
     3901 tggtttgagc tttccattaa cagagcagtt acatgataag gttttatctc tcccaataga
     3961 tccaacaatg accgattgtg atattgaata tgtaataaaa gttgtaaatg gctttgtcgg
     4021 atgaataagt tgctctcggt aacattcctt acaggcctac taactttcgc aaggatggtt
     4081 gctgggttta tcgttgcgaa aattgtagct atttatactg gcccgacagg aatcgcaatg
     4141 ttaggtcaat tgcaaaatgt aattaatggt gttagtggat tggttaattc accgcttaat
     4201 acatctattg tcagatttac tgcggaaaat tatagaacag gatatgaaga atgtgcaata
     4261 tggtggcgag taagtattag atgggctatt attatctatg cggtaattgc gccaatgatg
     4321 gttgtattat cgaagcagct ttcatctttt ctatttgaat ccgatttata ttattgggtt
     4381 ataatcgctt ctgctttagc attaccattc acagcggtag gaaccctaat taattctata
     4441 ataaatggtc agcaattata cattcaatat gttttgttag ggatggtgtc tgtcataatt
     4501 tcgtcgatga ttatgatcag tcttgttgta attaaaggag tagaaggcgc attaattgca
     4561 ttagccctcc agaatgcaat aattggcatt gctatgtttg gatatggttt acgaagacca
     4621 tggttcaaat tacaatattt tttcggaaat atatcaaaag agcaatcaaa aatcattctc
     4681 ggttatacat ttatggcatt ggtatcggca ttaacaatgc ctgtagtatt aatgttgata
     4741 cgtaaattct tagtcatgga agttggatgg gagcatacag ggtactggca agctgtctgg
     4801 aaaatatctg aaacatattt ggctgtggtt accatgtcat tatctatata ttatttacct
     4861 caactttcca gtattaacga tatcacaagc ttaagaaaag aaatttataa gacggttaaa
     4921 gttgttatgc ccatagtcat attaatggca tttgctatat atatgcttcg tgatattgca
     4981 atttctttat tgtttacgga aacctttaag ggggctagag atttattttt agttcagtta
     5041 tgcggtgatg tagtgaaaat tctttcatgg ctttttgctt atgcacttat ttctcgaggt
     5101 gaaactaaac tatttattgc ttctgaagct atatcagcaa taactttcat cttattggct
     5161 tttttttgta taaagttcta tggtgttcag ggcgcaaata ttgcttattt agctaattat
     5221 gtaatttatt ttatattgat ttattattgt gtgttttatc ggttaaaaag tcataggtgt
     5281 caataggtaa ataaaatgga attattggaa aaaataataa cagtctgtgt agtgacatat
     5341 aattcattta caacagtaat taaaacttta gaaagtataa aaaaacaaac ttatgataca
     5401 aattttattg aattaatcat tgctgatgat tgttcaagtg atgataccgt aattattgta
     5461 aaaaaatgga ttgaagagaa taagtgtata tttaataatg tggtgcttat tgaacaaaaa
     5521 caaaacattg gcttaactaa aaatatcaat tcagcatgga aattggcaaa aggcagttgg
     5581 gtgaaatcca ttgcaggaga tgatatctta ctcgaaaatt gcatagcaaa taatatcact
     5641 tatgtaaaca agcacaatat acaaagtgta gtcttttctt taatgcgctc ttttacagaa
     5701 gataataata aaataacttt tcatgaaata taccccaact cataccagca aaaagtgttg
     5761 aaaggagaac gagacgaaca aattaagtat cttactcatg ccggaggttt tgcctgtgct
     5821 ccctctgctt ttattaacag gaggttactt gaagaggttg gttttgcaga tgagcggttt
     5881 tttatgttag aagatacacc actttggttt cgcatactaa aaaaaggaga aaaaatatat
     5941 tttatgaaca tttttactgt aaaatacagg attggaaatt cgatatcgca gtcaaataag
     6001 tctttattta atgttcgtca tttgagtcaa aaaatggaag tcgattttct gttatttaaa
     6061 aatgaagtag gtctaatagc caatataaga aaattcatat tttattttat ttgttttttt
     6121 ttggttttta cgactggtgc aaataagaac tttataagca aaacagtttt tagattagct
     6181 ttgttagtta aacctttctg gttaagagaa aaaataaaaa atgctaaaat tatataaaaa
     6241 taaagcccaa gagtattatt tacttatttg cttgtttttg tgtgttttta acttttatgt
     6301 gccttctgtt ggtcctgctt tgtatatatc aattgcaatg gtttttgttt atcttgtcgt
     6361 caatattaag tcgttatgta aatataatag tgtaatatct agggcttatg tttcatatat
     6421 atttatatat ggattgcctt ttttattctt aatatgtttt gtcggagtta gaacaatatt
     6481 gtcagggagt ggtgatacat catttttgtt tttacttatt aagtattttg tttttggttg
     6541 tgtcgcatct ctatttgctg tggcaatttt tataagcagc gagatcacat cattaagcga
     6601 tgtgcaggct cttttttata gaattacgct ggttcaatcg ctaataatta tagctgcagt
     6661 gctgtctcct tctatttctg aatttgttcg attatatcag aataatgatg ctaacaactc
     6721 atcacttgta cttgaaggac ttagaggagt tgccttatca agtatgcaat tttattcact
     6781 tgcatgctat ttttgcataa taactatttt actggctgat gattttataa acaataaaat
     6841 aggatattta aagacgagtg tgtttttgtt gttaatggct atttcatcca tttttataag
     6901 ccgtaccgca attataggag ttttactttt ttttgtatat acattgaatc cattcataaa
     6961 atatggtaag aagcgtagcg tagctatggc tctaatgttt ttggtgttga cattagtatt
     7021 tttattattt tctggtttga tatttacaga gcaagtttct attattcaac aaaaagtttt
     7081 gccttgggcc tttgaattaa tttatagata tagcgagacc ggttcatttt ctaccgcatc
     7141 aactgatgaa ttgaagagta tgtatttctc attaaatgaa ataaccatgc ttattggaga
     7201 tggaaaatat acaggtgata cgcctggtac atactatatg ggaactgatg ctggctatat
     7261 gagaccaaca ctttttggtg gttttttttt gatattagcg atgttggtaa tatggatctt
     7321 gtggttgaaa aatacatttt accctaaaaa aggaggagta ttatttatct ccttggttat
     7381 attgtctctt atattacagt ataaaggaga gttcataatt acaaattact gcacattaat
     7441 tttattgtct accttactta tggtttctca gctgttgaag caaaaattta gggggtgagg
     7501 aatggcagta aagaaaaaaa tttgcttctt tgcaatagat ctttatgagc gaggtggtct
     7561 tcagcgagtc accatcgata tcgcaaatca gctttcaaac tattgcgaag tatatatttt
     7621 gacttttcag aatgagggct tcaatagttt cgcatataga ttagataaaa gagttcaagt
     7681 cacttattta aaacgaatgt gtaaaacgag aactttaaaa agaaaagctc aatctatact
     7741 tcagatgtgt catattagac gatatcttaa gttacataac tttgattcgt ttataagtgt
     7801 tgggatggct tctgtcgtat ggacttgcat cccccaatta atgcttcgtt gtcgatatat
     7861 atgttgggat catactagtt ttttacgcac tgatacatgg gccgtaagag gacgtagact
     7921 atcaagatta tttgcggata aaatcgtcgt tttaacttct aaagatgccg ctctttggaa
     7981 aagttctaaa gttagtgtta tatataatcc atctccattt gatattagga gggcagactg
     8041 tgaaatatcg aggtatagaa agaaaaatca aatagtatcc ttagggcgat ttgttccagt
     8101 taaaggtttt gacaggcttc ttgatatctg gaaaatcgta aattcaaaat gttcagaccc
     8161 attctccctt atgttaattg gtgaagggcc tctaaaggaa catttaactg aaagaattgt
     8221 taatgaaaat attcctaata tatcaattga accatttacc gaaaatatta atatagtgta
     8281 ttgtgagtca aagatacaat tattaacttc aacttttgaa ggactttcaa tggtcttaat
     8341 agaagggatg acttattgct tgcctgcaat atcttttgac atcccttcag ggcctggtga
     8401 aatagtagat gatggaatta caggttatct tgttgaagat aatgaaaatg aagtatttgc
     8461 agacaagatt atattactta tgtctaatga ttctttgctg tttaaattgt cagataactg
     8521 taagaaaaaa agaaatatat ttgacgccag tgagataatt gatcactggc ttagtttgct
     8581 aaatataaag tgtggaaaag catgaatata ttatttttat caaatgcctc aggatatgga
     8641 gggagtgaga gaactttaga gattttagct aaacatatgg ctaaaaaaaa taaagttact
     8701 gtatttgttc agaatgaaat acaccgtgat aaattgaatg aatctggcat ttgcgtaatt
     8761 gaggcagata aagggtggat tgtaaaaaaa atcataaagg atttgtattt gatttacgat
     8821 gaaattatga gaaatgacgg tgtgattgca aatacaaaca aagctgcgat gtatttagca
     8881 atactttctt ttttcttacc taaaagaaag ataaagaaaa gtattgtgtt cgtaagagat
     8941 tttcaatgga agtattgtca ttttatattt tgggctttga gtaattctat aatatgtgtt
     9001 gccagtcctg cggtaaaaga atacgtaaat aaatataaag ttaggccttt agtaattcca
     9061 aatcctattg aatttaaata taaagcacta tccttagatg ttggaaaaga agatatgtca
     9121 acacctatca ttttgtgccc tgctatgatt tcaagatgga aagggttgga gtatttaatc
     9181 aggtcattgg cattaattga aacagattgc cagctatata ttataggtaa gattgttgat
     9241 gaggaatatt atagtttcat taaggatgaa gttgttttat taaaactatc tgagagagtt
     9301 agatttgttg attttacatc tgatatatca aattattata aaatgtctac agtgatcgta
     9361 aatacatcta tatcagatta tggtggtcct gaaacttttg gacgaacaat tatcgaagga
     9421 tggtcattta aaaaaccagt agtttccttt gactgtggtg gtccaaaata tctcatacaa
     9481 aataatgttg atggagtttt ggttaaggag aaaagtgtat tcgatcttgc atgtgcatta
     9541 cagaaaatat tacatgatag aaattattcc aacatgttag gaatgaatgg ctacaacaaa
     9601 gctctgcaga aatataatgt ggatattata acgaaaacga tattaaaaga attatatgaa
     9661 acagccgttc gcaataaatg atttttgctt gcaggcaaat aagtttctta accatcattc
     9721 aatatggaga acgaatgtat ataattattg atgcaagaat gataaacgct tctggaatag
     9781 gcacatacat taagaactta ttacgtgaaa ttaataaata cgattcgatt agattacaat
     9841 gcatcataaa tcatgaacat atagaagaag tcaaaagtat aggtatcaat gattatataa
     9901 tttttaaggc tggatttttt tctccatttg aacagcttga atatatagtt aaattgcctt
     9961 ctgcagatat tttttggaca ccacatttta acacgtcaat tttctcttta aaggataaat
    10021 ttaaaaaacg tgttgtgaca attcatgata tgtttccttt tgcatatcga gatagttata
    10081 aaaaatccca ttttttctat attaaatttc ttatgaaaaa agctgctaag ataagtgatt
    10141 taattttaac agtatcaaat ttttctaaag ttcaaataat tgactatctt gatgtgagtg
    10201 aagagaaagt tttagttgta ccattgggcg ttgaccctga ttttttaaat acttctaatt
    10261 ctagcagtgt taaaaaaaaa tatattttat gtgttggtaa tgttaagcca cataaaaata
    10321 ttaagcgggc cattaaagca tttattgcag ttgccgatga aattccttca tatgagttag
    10381 tgatcgtcgg aaagtccgag gggttttact caccagaaga taatttaaag gaattaatac
    10441 agggtaataa aagagttcat tttacaggct ttgtgagtat tgaagaacta aaaaaatatt
    10501 attcagaagc atctatgctt ttatttcctt cactttatga agggttcggc ctgcctttac
    10561 ttgaagctat gtcattcgga ttgccaattg cagcctctgc aagagcttca ctaccggaag
    10621 ttggtaagaa tgctattata tactttgatc ctgaaagtgt tgatgatatt ggcaatgcta
    10681 tagtcggaat aataaatggt gatgttaagt gcaatacctt agcttatcaa tcaatactaa
    10741 acgaatacac atgggaaaaa tgtacgtcag aaaccttaaa tagcttcaga aaattggtac
    10801 tagaaaatga aagttcttca aataggtaag tttttttatc cggtgagggg tggggttgaa
    10861 acggtagctt atgatattgc agagctatta aattccaaaa aaataaaatg cgatgtattg
    10921 tgtacaaata aaaaccagac aacaaccatt gagttgcata atggttataa ggtatatcgt
    10981 tctgggataa ttggaagtgt gttttcaact caaatttctc tgcagtatat taaaacacta
    11041 aaaaacatca tcgatgatta tgatatttta catgtgcatt tacctaatcc gttagccaat
    11101 atcgcaatca ttttagctaa tactaaagat aagaaaatcg tgttacactg gcatagtgat
    11161 ataattaaac aaaaatattt gttaaaatta tatagacctt tccaaaactg gttgttaaaa
    11221 aaagctgatg ccataattgg tacatctcaa aattatattg ataattccag tgatttgttg
    11281 ccgtttagat ataaatgtgt cccaattcct attggaattg atcctcaccg tttattggca
    11341 gaacatcata aagtcaaaaa tataaaagaa aagttttcgg gaaagaaaat catattatca
    11401 cttggcagac acgtctatta taaagggttc aattatctta ttgaggcagc acaatatctt
    11461 agcgatgact atgttatttt aattggaggt gatggccctt taacttcggt aatgcatgat
    11521 agaataaaaa cactaaattt aaccaacaga gtatttctta ttggaagcat tccaggtaat
    11581 gatttaggtt cttattttga agcatgtgat gtattttgct taccatctat aatgaagtct
    11641 gaggcatttg gaatagtgca aatcgaagct atgagttttg gtaaacctat agttgccacc
    11701 aatattcaag gctctggcac cagttgggtt aatgagaata aagttagtgg tttaaatgtt
    11761 gatattaaga atccagagca aatcgccagt gctatatata atattacttg tgaatcagta
    11821 cgttatcggg agtattgcaa ttcatcattc gagcgattca atcaattttt tcatcgaaat
    11881 gttatgattg atcatattat taagttatac cagacactat aaccataaat tagcgagagt
    11941 gttatgattg tacctatcat tatagcgggt ggaacaggca gtagactttg gccattatca
    12001 cgtagccttt accccaagca atttttgccc ttggtaaatg aatcaacaat gatccaagat
    12061 actatcgaca gattaaagta tattgaacat ttagatccca ttattatatg taatgaagag
    12121 catcgtttca ttgttgcaga gcaactgagg gaaaaggaat ataagcataa tggcattata
    12181 ctcgagcctg taggaaaaaa tacagctcca gctgttgctc ttggtgccca tcgtgcgtta
    12241 gaaattacta atgatccaat attattagtt ttggctgctg atcactctat tttaaatatc
    12301 cataatttta gcgaagcggt taatttagct aacgaagcag ctatttctgg taaactagtt
    12361 acatttggaa ctgtaccttc tgctcctgaa actggctatg gatacattaa aaaaggtggt
    12421 ttaatatcaa atggtatata tcaagttgaa aaatttgttg agaaaccaga taggataact
    12481 gcacaaaaat atatcgaaag tcataattat ttatggaata gcggcatgtt tatgtttcat
    12541 gctaaatctt ttttaagtga actatgtaag ttcaattcta aaatgaacag tgcatgtaag
    12601 cagacatata ctaatgctaa aaaagatttg gagtttatca gattacctca tgatgacttt
    12661 attaagtgtc cagaggattc tgtagactat gctgtaatgg agcacactag caatgcagta
    12721 gttataccgt tagatgcaga atggaatgac gttggatctt ggtcatctct ttgggatata
    12781 agtgctaaag acatccaagg aaatggtaca aaaggtgatg tgattacaat taatacccaa
    12841 aatagttata tacatgccag cgatcgacta gttacatccg ttggattaaa tgatgttata
    12901 attgttgaaa caaaagatgc aatactggta gcaaataaga ataacgttca agatgtaaaa
    12961 gaacttgtta atagacttaa atctctaaat cgtaaggaat atattgaaca ttcacaatgc
    13021 tatagacctt gggggcagca tgaaagtata gctcatgggc caaggtttca tgtgaagcac
    13081 gttatcgtaa agcctggcga aaaaattaca gagcaagttc attttcatag aactgagcat
    13141 tggattgttg tttcaggtac agcaaaagtt acgaaaaatg aagtatcttt tattatgaaa
    13201 gaaaacgaat caacatttat tcctgcgggt acagtgcata ctttagaaaa tccaggtaaa
    13261 attcaactcg aggtaataga aatccaatca ggaaattatt tagatgatga tgatattctt
    13321 cgttttgacg taaaaaaata agaagggaaa tcatatgaat aattaccttt taaaatctaa
    13381 agcgttaatc gtgaattgca atgttgtatt tggcacaagt ggtgctcgcg gtttagtgat
    13441 cgatttttcc catgatgttt gtgctgcgtt cactcatgcg tttctttctg ttattgatga
    13501 caaatacaat tttaataaag ttgccttagc cattgataac cgcccaagta gttacgaaat
    13561 tgctcaggca tgcgcgtatg ctataaaaca acatggattc gaggttgaat atcatggtgt
    13621 aattccgact cctgcattag ctcattattc gatgcagaaa aacattccct gcataatggt
    13681 cacagggagt catatccctt ttgatcgtaa tggcttaaaa ttctacagac ctgatggtga
    13741 aatcactaaa gaggatgagc tcgcaattat aaatagtgag tatacatttt cgcctgtagg
    13801 tgtattacct catcttgaaa caagcactca aggtgcagac tactacctcg agcgttatgt
    13861 ttctcttttt aatcccgaga ttttaaaggg gaaaagaata ggggtatatg aacattctag
    13921 tgcaggacgc gatttatatg ctcctctttt taatcaattg ggtgcagagg tcatttccct
    13981 cggtagaagt gatgagttcg ttccgattga tacggaagca gtaagcgatg aagatcgtat
    14041 acttgcaaga gagtggtcta aaaaatataa ccttgatgct attttctcaa cagatggcga
    14101 tggtgatcgt cctttagttg ccgatgaaaa tggtgaatgg ttaagaggcg atattctggg
    14161 attgcttact gctattgaac ttaatatcaa ggcgttggct attccagtga gttgtaatac
    14221 agcaattgaa gagtctaata aatttgcaag tatacaacga acgaaaatag gttctcctta
    14281 tgtaattgca gcgtttgcag atcttgctaa gcaatttgat tcagtcgctg gttttgaagc
    14341 taatggtggt tttctccttg cctccgattt acaaattaat ggaaaggaat taaaatcgtt
    14401 acctacacgg gatgctgtgt taccagcatt aatgctctta atagcctctc gcaatagtac
    14461 tatatctcaa ttgattaata atcttcctca gcgattcact tggtcagata gagttaaaaa
    14521 cttcccttca gattcaagtc aacaaattat aaaaaatgcc atatcatcac ccaataattt
    14581 ctttaataat ttaggttacg aatcattatc ctgttccgct attgatgaaa cggatggtgc
    14641 aagatttact ttaaataatg gtgatattat acatctccgt ccttccggta atgctccaga
    14701 actccgttgt tatgctgagg ccagtgatga aaatcaggct aagcaatatg ttgcgaatgt
    14761 gctgggaaat attacctctt tgatttcttg atgttataga tttatctacg cttatatgtg
    14821 tgcgtaggtt tgattacacg tagatgccgg tatacagaat tgaagaacgg tatttgttgc
    14881 attaatgaaa ttcagcacta cacacattcg tgcaacttga gataacatct caatcatatt
    14941 caagtcgcgc atacatcgcg gtgaacaccc cctgacagga gtaaacaatg tcaaagcaac
    15001 agatcggcgt cgtcggtatg gcagtgatg
//