<pre>LOCUS       KJ778802                9657 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain SSI 81930 serotype O182:K48:H25 O-antigen
            gene cluster, complete sequence.
ACCESSION   KJ778802
VERSION     KJ778802.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9657)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9657)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Swimley,M.,
            Baranzoni,G.M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SSI 81930"
                     /serotype="O182:K48:H25"
                     /db_xref="taxon:562"
     misc_feature    1..9657
                     /note="O-antigen gene cluster"
     CDS             86..895
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase (GNAT) family protein"
                     /protein_id="AIG62781.1"
                     /translation="MININMRKNNASREYLDICWDTFFKSKNRGLDLVAHFPWLTSGS
                     SSFISYEVEKNNQIVGGLIVLERDIRNLKIGLVGLVCVKKECRGQGIFKALMRKMLIS
                     CKESHFSALYLWSSKHSIYEKFDFELHDKNILVKIKVINDGDFGKPILNNYGDVNIIH
                     RTDLAVPPFASGIYELKTECGSIVYCESNGNKYIINHENTVENVILEFGKFNKGEFFL
                     NTIIDRKNNILSMKDYFDVEIIRQNIQMVNLIDKASIDKVYKINFLVNERI"
     gene            898..1995
                     /gene="arnB"
     CDS             898..1995
                     /gene="arnB"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate
                     aminotransferase"
                     /protein_id="AIG62780.1"
                     /translation="MIPIVKVFMPPKDVLMPALEQVLYSGMVGEGKHVYTFEDKFKKL
                     FGINNAIAVSSGTAALHMALILAGIKKGDEVITTSMTAEPTNTSILQAGGVPVFADVD
                     KNTGCLDPDSIIEMISDKTKAILLVHYAGYLVDVPELKEKLRRKINRKIKIIEDCAHA
                     LGATIDGRMVGTFGDYGIFSFQAIKHMTTIDGGFLVIKDTSQTKLAKKMRWFGMEKGV
                     ERTSLDLDVLGFKYNMNNVTGVIGEIQLDYVKDNISRHQKNAQFYNMMLNDITGIEVV
                     ESRQSHSPSYWLYTLLCDDSDDIINKLNEINVAASKLHRPNHYHSLLSQCRRASMENL
                     DSFYKRLVHLPCGWWIDEITRSKIIEALRRG"
     gene            1997..3148
                     /gene="wbpE"
     CDS             1997..3148
                     /gene="wbpE"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2-acetamido-2-deoxy-3-oxo-D-glucuronate
                     aminotransferase"
                     /protein_id="AIG62779.1"
                     /translation="MINLFNVSFDHDIQKVVEDTLVSKAIASGAHVCQLESRLSDLFS
                     EKYALTVNDMTNGITLLLDVFGLKKGDEVLVSPFSCLATTSPMALLGIKPVWVDIEPS
                     TLAMNIKSIESNITKKTKAILVYHVAGYPSDIVEISKIAKKNNLLLIEDCNSAFGSKY
                     RNKSLGSWGDATVLSFYPNRQVGSIDGGCVLFKDKNHYELAKLRRRYGVDFNDFRKVN
                     GEINEAKDVETYGYSATINNVSAGIIMAKLPKYANEMLLTQKNVSKLNNVVMSLQNKY
                     KGSIKLVTPLKDSCVNNWVYFVRVRNKESFMNEMKKKGVHVSSLHLRNDLYSCFHAER
                     KHLTGVDIISDTLVAIPCGYWISEEDIDIIGKYIEQTLIFLVKNETSYN"
     gene            3129..4499
                     /gene="wzx"
     CDS             3129..4499
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AIG62778.1"
                     /translation="MKHRIIDLGMLYFGKFGALAVGFFLMPAYSYYLGPASFSTVAFI
                     LSLVNAAVTLDFGMSTIIGRDSSDSTISNDKKYKQFLASQFLVIFFYLSVFSIVAIYS
                     FLFQKGDLGVKISFLSVILIMSSLIQNISVSYLNGIKEFKISGSLIFLSVTLRGVISL
                     LVIKYIDNNIISFMSAQSIIGLLFSLIFMLLPSRLDKEIKINNRWGGFFEPSIYIKFL
                     LPLVKKGFPLLFMGVSATLVMQFDKIILSYYASDATLSAYYLAFTFSTIPILAIAGPI
                     KQYFQPHIVSYLTQNNVLYRKNSIVFFWCLIVFVAVPSALGYSSLYQILSIWLGNNEL
                     IDKVDFFSHILLPAFVLGAISYFPSVLLVVAEDYRFQSVFAIITSIIFVFLLFILAIS
                     SNVRFIPWLFIGYFCVVILFCSIRCFSLTKVNLCIFDMVKNSPFAILIIALSYWVGNI
                     VSAMIF"
     gene            4553..5563
                     /gene="capD"
     CDS             4553..5563
                     /gene="capD"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="AIG62777.1"
                     /translation="MFKDKTLMITGGTGSFGNTVLKRFLNTDVKRIIVFSRDEKKQED
                     MRIALANPKLKFIIGDTRDYESVYRAMNGVDYVFHAAALKQVPSCEFYPMEAVKTNVN
                     GTENVLNAAIDCGVKRVVLLSTDKAVYPINAMGISKAMAEKVLIAKARRQDNDKTIMC
                     ATRYGNVMASRGSVIPLFFEKIKLNKPLTVTDPNMTRFLMSLEDSVDLVLHAFKNGRQ
                     GDIFVQKAPACTIGDLTEAIKQLCDSNIETKIIGTRHGEKLYESLVSREEMVKAEDMG
                     RYYRIPADNRDLNYDKYIIKGQIDINKLDDYTSHNTERLNVEQIKALLLTLKYVQKEL
                     GI"
     gene            5624..6778
                     /gene="pglI"
     CDS             5624..6778
                     /gene="pglI"
                     /codon_start=1
                     /transl_table=11
                     /product="galNAc(5)-diNAcBac-PP-undecaprenol
                     beta-1,3-glucosyltransferase"
                     /protein_id="AIG62776.1"
                     /translation="MIKLSILIPTKNRSEYLDDVLSSLLTWKRDDFEVIIQDNSDDLK
                     THNVVEKYRYDHRVKYFYVKEHLSVIDNFEYAVKNSSGKIITAIGDDDGILPIVMDVA
                     DWMLKNSVEAVLTNKAEYVWPDLISKYNSNYNNSKLKFNKNYSYDYKKINCIDEFLQV
                     CRHGGTSMGRLPRIYYGLVCRESLDHVFNLTGQYFPGPSPDMANAASLSLVIKSFYYL
                     NFPVFIAGSSSKSTAGLGLKRKHLGAISEIKHLPMNTMAEWNKNIPDFWSGPTIWAQS
                     VSSVIKKMEPTYDLNYEYLYARCFVFFPQKSKCIYLALRQYGISGKTVIKIIYWYVYN
                     WKLRFVHLLPKLLAKIGKKDHNIITIDEQLKISQAVDSLLSFTQKSDLSK"
     gene            6775..8001
                     /gene="wzy"
     CDS             6775..8001
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AIG62775.1"
                     /translation="MSENILSRLTLNSAQKCLLTGLIIILLGPYGGALGFALVVCGAG
                     LEKGRIERYILCVFSFFIFTLCSVQGVFHSSNFNTISLFTALCTVMVLFLVDNNSLIL
                     ILDKLIFVISVTVPIALIIAISQTLGVYPFEFRSNEVLGVMRFTFLFAEPSHYSILLS
                     LCSLISLVKKTNLFTRFILLFGLLMTWSLSGFCIFTFGYIAYKFYTRGIIKYFIISLL
                     LGSIITFLWFFKLQYSDFWLVAKIDSIIQLFSGNTVGASSALLRLNSIIIGPYFINEK
                     INSDNFLLSFFGLGFGNLSDFVSVYYQSNFGLPDVYDANNIISNVIISSGIVGLVFYI
                     LSFSYFTGLNLVNILFAAIILLCLSLFSGYAFGPFAILTLLLSRAFILLVINHDKARI
                     NRCTYFNTWFENKTGS"
     CDS             8081..8719
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="AIG62774.1"
                     /translation="MLNRTDVIYIRSTSNGVTKSRNILLDKADADLIYFCDDDVVLSE
                     NFDEIIINSHKKYSDDVILFNIRDENGELRKKYPNEVVKKTRFNILSVGTIEISLKNS
                     PLLPKFPEDMGAGTSLPVGDEAVFLSTFIKKGKSIRYIPNTIAIHPRESTGLEVTNGS
                     IYSRGVALNRIYGIYAFLVAIFFLLRRRSLLKTDRGVLQGGILFFKGVLLGK"
     gene            8732..9481
                     /gene="tuaG"
     CDS             8732..9481
                     /gene="tuaG"
                     /codon_start=1
                     /transl_table=11
                     /product="teichuronic acid biosynthesis
                     glycosyltransferase"
                     /protein_id="AIG62773.1"
                     /translation="MKISIVTATYNSWPYIKETYDSIANQSYKNWEWHVTDDCSSDYT
                     YQYLKQLADRDCRIVIHRNEINSGAAVARNKSLSQCSGDYIAFIDSDDLWNPEKLESQ
                     LKFMVDNQLDFSFTGYEVIDESGKSIKKYIDINQTQAISYEDMLKKKATLGCSTVMLR
                     RLAFSELSMPDLRTGQDYALWLKLLKTGIRAVPYNKSLMKYRIVSSSISRNKYRKAKR
                     QWQIYRELEKLSLVKSIYCFLFYAWRAIFRK"
ORIGIN      
        1 tagacatgca tgcagtgctc tggtagctgt aaagccaggg gcggtagcgt gttttattta
       61 ctgcatgtat ttgagaaaag tagacatgat aaatattaat atgcgtaaaa ataacgcaag
      121 ccgagagtat ttagatattt gttgggatac tttcttcaaa agtaaaaata gaggcctcga
      181 tcttgtcgct catttcccgt ggctaacttc agggtcgtct agttttattt cctatgaagt
      241 cgaaaaaaat aatcaaatag ttggtggtct cattgttcta gaaagggata taaggaactt
      301 aaaaattgga cttgtgggtt tagtttgcgt caaaaaagaa tgccggggac aaggtatttt
      361 taaagccctt atgcgtaaaa tgctgatatc gtgcaaagag tctcattttt cggcattata
      421 tctttggtcg tcaaagcata gtatttacga aaagtttgat tttgaacttc atgataaaaa
      481 tatcttagtc aaaatcaagg tgataaatga tggtgatttt ggaaaaccca tattaaataa
      541 ttatggagat gtaaatatca tacaccgcac agatttggct gttccacctt ttgcatctgg
      601 tatttatgaa ttaaaaactg aatgtggctc tattgtatat tgtgaaagca atggtaataa
      661 atatataatt aaccacgaaa atacagtcga gaatgttata cttgaatttg gaaaattcaa
      721 taaaggtgag ttttttttaa ataccataat cgatcgaaaa aataatattt tatctatgaa
      781 agattatttc gatgtggaaa taataagaca gaatatacag atggttaatt taattgataa
      841 agccagtatt gacaaagtgt ataaaattaa ttttttagta aatgaaagga tttagtaatg
      901 attccaattg ttaaagtttt tatgccacct aaagacgttt tgatgcctgc tttagaacag
      961 gttctttaca gtggaatggt cggtgaaggt aagcatgttt atacctttga agataaattt
     1021 aaaaagctct ttgggattaa taatgctatt gctgttagta gcggtactgc ggcattacac
     1081 atggcattaa tcttggctgg tataaaaaaa ggcgatgaag ttattaccac cagcatgaca
     1141 gcagaaccaa caaatacatc aattttgcaa gcaggagggg ttcctgtttt tgctgatgta
     1201 gataaaaaca caggatgtct tgatcccgat tctattattg agatgataag cgataaaact
     1261 aaagctatat tacttgtcca ttatgcagga tatctggttg atgttcctga actaaaggaa
     1321 aaattaagaa gaaaaataaa tagaaaaatt aagattattg aagactgtgc acacgcttta
     1381 ggcgcgacaa ttgatgggag aatggtagga accttcggtg actatggcat attctcgttt
     1441 caggctatca aacatatgac aacaatcgat ggtgggtttc ttgtaattaa ggatacctct
     1501 cagacgaaat tggctaaaaa aatgcgatgg tttggcatgg aaaagggagt ggaaagaaca
     1561 tctcttgacc tagatgttct gggatttaaa tataatatga ataatgttac tggtgttata
     1621 ggcgaaatac aacttgatta cgtaaaagat aatatatctc gccatcaaaa aaatgcccaa
     1681 ttttataata tgatgctgaa tgatattacg ggcatagagg tagtagaatc taggcaaagc
     1741 cactcaccga gttactggct ctatacttta ttatgtgatg attctgatga tatcattaat
     1801 aaattgaatg aaataaatgt ggctgcttcg aaactacata ggccaaacca ttatcattca
     1861 cttctatcgc aatgcagaag ggctagcatg gaaaatcttg attcattcta taaaagatta
     1921 gttcatttac cttgtggttg gtggatcgac gaaataacac gtagcaaaat tattgaagca
     1981 ttgagacggg ggtaatatga ttaatttatt caatgtctca tttgaccatg atattcagaa
     2041 agtggtagaa gacactctgg tttctaaggc tattgcatca ggtgcacacg tatgtcagct
     2101 agaaagtagg ttatctgatt tattttcaga aaaatatgct ctgacagtaa atgacatgac
     2161 taatggtata actttattat tagatgtctt tggacttaag aaaggagatg aagttttagt
     2221 atcacctttt agctgtttgg caacaacttc accaatggcc ttgttaggta ttaaacctgt
     2281 atgggttgat attgaaccat caactctggc tatgaatatt aaatcaatag aaagtaacat
     2341 aacaaaaaaa actaaagcaa tattggtata tcacgtggca ggttatccca gtgatattgt
     2401 tgagatttct aaaattgcga aaaaaaataa cctattgtta attgaagatt gtaattctgc
     2461 atttggaagc aagtatagaa ataaatcgct tggttcttgg ggggatgcta cagttttatc
     2521 attttatcca aatagacaag ttggttctat tgatggagga tgtgtacttt ttaaggataa
     2581 aaatcactat gaactggcga aattaaggag acgctatgga gttgatttta atgatttccg
     2641 taaagtaaat ggagaaatta atgaagctaa agatgtagag acttatggct attcagcaac
     2701 aattaataac gttagtgctg gaattattat ggccaaacta cctaagtatg ctaatgaaat
     2761 gttgctcacc caaaaaaatg tatcgaaatt gaataatgtt gttatgtctc tacaaaataa
     2821 atacaaagga agtattaagc tggtgacacc tttaaaggac tcatgcgtta ataattgggt
     2881 ttattttgtt cgtgtaagga ataaggaatc tttcatgaat gaaatgaaaa agaaaggagt
     2941 gcatgtatcg tctttgcatt taaggaatga tctgtattca tgttttcatg cagaaagaaa
     3001 acatttaaca ggggttgata ttatatcaga tacactagtt gcaatacctt gtgggtattg
     3061 gatatcagaa gaagacatcg atattattgg aaaatatata gaacaaacat taatattttt
     3121 ggttaaaaat gaaacatcgt ataattgatt tggggatgtt atattttggc aagtttggcg
     3181 cgttggcagt tggttttttt cttatgcctg cgtatagtta ttaccttggc cctgcttcct
     3241 tttcaactgt cgcctttatc ctttcgttag ttaatgcagc cgtcacatta gattttggca
     3301 tgagtactat aattggaaga gattcttctg atagtacaat tagtaatgac aagaagtata
     3361 aacaattttt agcatcacaa tttcttgtga tattttttta tttatctgta ttttccattg
     3421 ttgctatata ctcttttctt ttccaaaaag gagatttggg ggtaaagata agttttctgt
     3481 cagttattct tatcatgtca tctctaattc agaatatttc cgtatcttat ctgaatggaa
     3541 ttaaagaatt taaaattagt ggttctctta tttttttgtc agttacatta cgaggggtaa
     3601 taagtctttt agtaataaaa tatattgata ataatattat atcatttatg tctgctcagt
     3661 ctataatagg gcttttattt tctttgattt ttatgctttt accttctaga ctggataaag
     3721 aaataaaaat aaataataga tggggtggtt tttttgaacc ttcaatctat ataaaatttt
     3781 tgctaccttt ggtaaagaaa ggtttccctt tgctttttat gggagtatcg gctacattgg
     3841 ttatgcagtt tgataaaatt attctttcat attacgctag cgatgcaacg ctttcagcct
     3901 attacttagc atttactttc agcacaatac ctatacttgc gatagctgga cctattaaac
     3961 aatattttca acctcatata gttagttatt taactcaaaa taatgtgctg tacaggaaaa
     4021 atagcattgt ctttttttgg tgcctgattg tttttgttgc agtaccttca gcattaggat
     4081 attcatcgtt ataccaaata ttatctattt ggcttggaaa taatgagttg attgataaag
     4141 tagacttttt ttcacatatc ctgttgcctg cattcgtttt gggcgctata agttattttc
     4201 cgtctgtatt attagtggtt gcagaggatt atagatttca gtctgtattt gcaattatca
     4261 cgtctattat ctttgttttt ttattattta tacttgctat aagtagtaat gttcgtttca
     4321 taccatggtt gtttataggt tatttttgtg tggttatttt attttgcagt ataagatgtt
     4381 ttagtttaac taaagtcaat ttatgtatat ttgatatggt aaagaacagt ccttttgcaa
     4441 ttctaataat tgcgctgtcc tattgggttg gtaatattgt atcagcaatg attttttaat
     4501 gattgcttat attatctata tatatcaatg ttaattaaag ctgggattaa taatgtttaa
     4561 agataagact ctaatgatta ctggtggtac tggatcattt gggaatactg tgctgaagcg
     4621 ctttcttaat acagatgtaa aacgaattat tgtttttagt cgagatgaga aaaaacaaga
     4681 agatatgcgg attgctctcg caaacccaaa actgaaattt attattggtg atactcgtga
     4741 ttatgaatca gtataccgcg caatgaatgg ggttgattat gtattccatg ctgcagcatt
     4801 aaaacaagtt ccatcttgtg aattttaccc gatggaagct gttaaaacta acgttaatgg
     4861 gactgaaaat gtattaaatg cagctattga ttgtggcgta aaacgtgttg ttttacttag
     4921 tacagataaa gccgtatatc ctataaatgc gatgggaata tcaaaagcga tggcggaaaa
     4981 ggtgctaatt gcaaaagcta gacggcagga taacgacaaa acaatcatgt gtgcaacccg
     5041 ttatgggaat gttatggcat cccgtggctc agtaatacca ttgttttttg aaaaaataaa
     5101 actcaataaa ccattgactg taactgatcc taatatgaca aggttcctca tgtcccttga
     5161 agattctgtt gatcttgttc ttcatgcatt taaaaatgga agacaaggcg atatttttgt
     5221 tcaaaaggca cctgcttgta ccattgggga cttaacagag gccataaaac agttatgtga
     5281 cagtaacata gaaactaaaa taatagggac acgtcacggt gaaaaacttt atgagtcgct
     5341 ggtttctcga gaagaaatgg taaaagctga agatatggga cgatattatc gcattccagc
     5401 ggataatcga gacttaaatt atgataaata tattattaaa ggtcagatag atatcaataa
     5461 acttgatgac tatacttcac ataacactga acggttgaat gttgaacaaa ttaaggcgtt
     5521 gcttttgaca ttgaaatatg ttcaaaagga acttggcatt taaataaatt gttatgttgc
     5581 aaatccaata ataatattgg atttgcttat ctgagtggtt tatatgatca aacttagtat
     5641 cttaatacct acgaaaaatc gtagtgagta cttggatgat gttttatcga gtttattgac
     5701 ttggaaacga gatgattttg aagttattat tcaagataat agcgatgatt tgaaaacaca
     5761 taatgttgta gaaaaataca gatatgatca tcgcgttaag tatttttatg ttaaagaaca
     5821 tctatctgtg attgataatt ttgagtatgc cgtaaaaaat agctcaggaa agatcattac
     5881 agcaattggt gatgatgatg gaatattacc tattgtaatg gatgttgcgg attggatgct
     5941 gaaaaattca gtagaagcgg ttttaactaa taaagcggag tacgtgtggc ctgatcttat
     6001 aagcaagtac aactcgaatt ataataatag taaactcaaa tttaataaaa attattctta
     6061 tgattataaa aaaattaatt gtatcgatga gttcttgcag gtttgtcgcc acggaggtac
     6121 cagtatggga aggttaccac gcatatatta tggccttgtc tgtagggaat cccttgacca
     6181 tgtatttaat ctaacaggac aatatttccc cggaccaagc cctgatatgg ctaatgcagc
     6241 aagtttgagt ttagttataa agagtttcta ttatttaaat tttccagttt ttattgctgg
     6301 ttcatcctca aaaagtacag ccggtttggg gttaaaacgt aagcatcttg gtgctatatc
     6361 cgaaattaaa cacctaccta tgaatacaat ggctgagtgg aataaaaata tacccgattt
     6421 ttggtctggt cctactatat gggcacaaag tgtttcaagt gtaataaaaa aaatggagcc
     6481 aacttatgat ttaaattatg aatatctata tgcccgatgt tttgtatttt ttcctcagaa
     6541 aagcaaatgt atttatttag cattaagaca atatggtata tcaggaaaaa ctgttatcaa
     6601 aataatatat tggtatgtat ataattggaa attaagattt gttcatttgt tacctaagct
     6661 tttagctaag atagggaaaa aagatcataa cattatcact attgatgaac aattaaaaat
     6721 atcacaggca gttgactcgt tactttcttt tactcaaaaa agtgatttaa gcaagtgagt
     6781 gaaaatatat tatccagatt gacattaaat agtgcccaga aatgtctact gacaggatta
     6841 attattatac ttttggggcc gtatggtggg gcgttgggat ttgctctggt tgtctgtggg
     6901 gcaggattag aaaaaggccg aattgaaaga tatattttat gtgttttttc tttcttcata
     6961 tttacattat gctcagtaca gggcgtattt cactcttcta attttaatac tatatcttta
     7021 ttcaccgcat tatgtacggt gatggttcta ttcttggtgg ataacaattc attaatattg
     7081 attctggata agttaatatt cgtaatatct gtaactgttc caatagctct gattattgcc
     7141 atttcacaga ctttaggtgt atatcctttt gaatttagga gtaatgaagt tcttggagta
     7201 atgcgattta catttttatt cgccgaacca tctcattatt ctatactttt gtctttatgc
     7261 tctttaattt ctttggtgaa aaagacaaat ttatttacaa ggtttattct tttatttgga
     7321 cttttgatga catggtcact atctggtttc tgtattttta cttttggtta tattgcttat
     7381 aagttttata caagaggcat aataaaatat tttatcattt cactgcttct cggatctata
     7441 attacttttt tatggttttt taaattacaa tatagtgatt tctggttggt tgctaaaatt
     7501 gattcaataa ttcaattgtt ctcaggtaac actgttggtg caagcagtgc acttcttaga
     7561 cttaactcga ttattattgg cccatatttc attaatgaga aaattaactc agataatttt
     7621 cttttatcct tttttggatt aggttttgga aacttatctg actttgtctc ggtatattat
     7681 caatctaatt ttggattgcc agacgtctat gatgctaata atattatttc aaatgttata
     7741 atatcaagtg gtatagttgg attggttttc tatatccttt cctttagcta cttcactgga
     7801 ttaaacttag ttaatatatt gttcgcagct ataatattac tatgtttgtc tttgtttagt
     7861 ggctatgcat tcggaccatt tgcgattctt actttacttt tatcaagagc ttttatttta
     7921 ttggttataa atcatgacaa agcacgcatc aatcgatgta cttatttcaa cacatggttc
     7981 gagaataaaa caggttccta atatattaca tgattttaaa agaaatgttc gctatataat
     8041 ctgtcaccag aattttgaac tatatgaacc aaacgagatt ttgttaaata gaactgatgt
     8101 aatatatatt aggtcaacaa gtaatggggt gactaaaagt agaaatattc ttttagataa
     8161 agcagatgca gatttaattt atttttgtga tgatgatgtt gtattaagtg aaaattttga
     8221 tgagattatt ataaatagtc ataaaaaata ttctgatgat gtaattcttt ttaacattcg
     8281 ggatgaaaat ggtgaacttc gtaaaaaata tccaaatgaa gtagtaaaaa aaacgcgatt
     8341 taatatttta tctgttggta caattgaaat atcgctaaag aacagcccgt tattacctaa
     8401 atttccggag gatatggggg cagggacatc attacctgtg ggggatgagg ccgttttttt
     8461 gtctaccttt atcaaaaaag ggaagagtat acgttatata cctaatacaa tagctattca
     8521 tccaagagaa tcaaccggtc tagaggtaac taatggttct atctactcta gaggtgttgc
     8581 tcttaatcgt atttatggta tctatgcatt tcttgtggct attttctttc tgttgagaag
     8641 aaggagcctt ttaaaaactg atagaggagt tttgcagggg ggcattttat tttttaaagg
     8701 cgtcttactc ggtaagtaaa aaggaatagt tgtgaaaata tctattgtaa cagcaacata
     8761 taattcatgg ccatatatta aggaaactta tgattcaatc gcaaatcaat cttataaaaa
     8821 ttgggaatgg catgtaacag acgattgttc gtctgattat acctatcaat atctaaaaca
     8881 attagctgat agagattgta gaattgtaat tcatcgaaat gagattaata gtggagcggc
     8941 agttgcaaga aataaatcgt tatcgcaatg ctctggagat tatatcgctt ttatagatag
     9001 cgatgacctt tggaacccag agaaactcga aagccaattg aaatttatgg ttgataacca
     9061 attagatttt tcatttacgg gatatgaggt tattgatgaa tctggcaagt caataaaaaa
     9121 atatattgac attaatcaaa cacaagctat tagctacgaa gatatgctaa agaaaaaagc
     9181 aacgttgggt tgctcaactg ttatgcttcg taggctggct ttctccgagc tttccatgcc
     9241 tgatcttcgc actggtcagg attatgcatt atggttaaaa ttattaaaaa ctggaattag
     9301 agcagttcct tataataaat ctttgatgaa atatcgtata gtttcctcct ctatttcacg
     9361 aaataaatat cgtaaagcaa aacgacaatg gcaaatatac cgcgagctag aaaaactttc
     9421 tttggttaag tctatctatt gttttttatt ttatgcttgg agagctattt ttaggaaata
     9481 agtcaatttt atttgccatt tttctaaaag gaattaaaat attgagaagt gtaaataata
     9541 acgagtagtc atcaataaat aatagagtta ttaacttacc gcatatcatg tggtaccccc
     9601 tgacaggagt aaacaatgtc aaagcaacag atcggcgtcg tcggtatggc agtgatg
//

</pre>