<pre>LOCUS       KJ778801               11843 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain 92-1250 serotype O181:H49 O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ778801
VERSION     KJ778801.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11843)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11843)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Swimley,M.,
            Baranzoni,G.M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="92-1250"
                     /serotype="O181:H49"
                     /db_xref="taxon:562"
     misc_feature    1..11843
                     /note="O-antigen gene cluster"
     gene            134..1177
                     /gene="tagE"
     CDS             134..1177
                     /gene="tagE"
                     /codon_start=1
                     /transl_table=11
                     /product="putative poly(glycerol-phosphate)
                     alpha-glucosyltransferase"
                     /protein_id="AIG62762.1"
                     /translation="MKIAFVGYNPGKIGGIEAFSRNMKNDIFPESEFVYEYDEAGTFK
                     VDNCVPLLKRNLFNRVLNRLTNGNYTKSKIKKKLKEKKYDIIILNSPKYLDVSPDLCR
                     TILVQHTTLDNWWKSKYNFGSNRNLAKLVKKVGKVVALSEMEGQVIKNFFRVEANKIR
                     VINFSAGIPFLYGKKEPGKNLIMLARFQNEIKRIDLVIEAMDDLPDYTLNVYGDGKDR
                     DYLIELSKHKKNVKIHSSITDKTSVLDKNNIYVLSSEYEGYPVSVIEAISRKLPVVAR
                     NTFYSASELVKDNGILLDKTWSKDEFCNAVRSCYENYEQYSNNSESYFYKYDITTIKN
                     NWVSLVQELNYAQ"
     gene            1167..2171
                     /gene="cps12A"
     CDS             1167..2171
                     /gene="cps12A"
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide phosphotransferase"
                     /protein_id="AIG62763.1"
                     /translation="MRNDIDVVITWVDGNEPDWIKEYTYYKGCEPGRFRDIGVLKYIF
                     RSLEKNMPWVRKIHFVTNGQYPTWLNLEHPKIYFHAHKDFFIYKDALPVFNSNAIELN
                     FANIPDLSERFILFNDDMLVLKQVKEERFFDNGLPVDYCKLSYPRKGRLYKWLKPQNY
                     LACELINNNYLYLKNTRVNQINKKNLYNKDYNLSTNISNFIFSIFRKVKWFEVYHHPQ
                     PHLKSTWLDKTSELRNAVVRNTIYTKFRAKTDVSHYLYRYQNLLNNKFTPRQYNDHYS
                     IYVKNVNEFNKNIKKYFSNTFLCICEDENMGDTEFIKLRRILIKELEIMFPNKCSYEL
                     "
     gene            2225..3262
                     /gene="capD"
     CDS             2225..3262
                     /gene="capD"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="AIG62764.1"
                     /translation="MFKSKVLLITGGTGSFGNAVLKRFLETDIKEIRIFSRDEKKQDD
                     MRKFYRSEKLKFYIGDVRDYQSISNAMRGVDYVYHAAALKQVPSCEFYPLEAVKTNVL
                     GTENVLEAAINHSVKRVVCLSTDKAVYPINAMGISKAMMEKVIVAKGRNLSESNTVIC
                     CTRYGNVMASRGSVIPLFIRQAVNGQPITITDPNMTRFMMTLDDAVDLVLHAFKNGKS
                     GDTFVQKAPAATIDILVKSLLSFLNKEDHPITVIGSRHGEKQFEALCSREEMLVAEDQ
                     GRYYRIPADNRDLNYSKYFEVGEKNLHLIQDYNSNNTEQLDVMGMKNLLRKLDFMREI
                     EAGNIVIPEGV"
     gene            3265..4143
                     /gene="rmlD"
     CDS             3265..4143
                     /gene="rmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="AIG62765.1"
                     /translation="MKVLILGASGMLGYSLYANMSEYHEFEVFGTVRDIAGKEHYYNK
                     PKGYIIKNVNILDLDKVKQIVAELKPDVVINCIGLIKQLSISKKYTSAITINALLPHQ
                     LAEICSSYEAKLIHFSTDCVFDGKKGNYKEDDLPTATDLYGKTKHMGEVYYGGHITLR
                     TSIIGHELISSISLVEWFLNQEKEVKGFTKAIFSGLPTAYIAKLLAEKIIPNKSLSGL
                     YHLSTFPINKYDLLTKISKVYDKNINIYKSDEVIIDRSLNSEKLRSEIGFVPPDWDEL
                     IEYMNADFKKWYTLCR"
     gene            4122..5273
                     /gene="wbpI"
     CDS             4122..5273
                     /gene="wbpI"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacetamido-2,3-dideoxy-D-glucuronate
                     2-epimerase"
                     /protein_id="AIG62766.1"
                     /translation="MVHVMQMNKKMKVATIVGTRPEIIRLSRIISRLDECTEHTLIHT
                     GQNYDYELNQIFFNDLNIRAPNIFLECAGDTPAETIANVISKSDQLFEKLKPEALLIL
                     GDTNSALSAISAKRRKIPIFHMEAGNRCFDLRVPEEINRKIVDHIADINLPYSSIARE
                     HLLREGLNPDLVIKTGSPMDEVLNFYASKIDASLSLQKMNVEENKYFLVSVHREENVD
                     SDVNINNFVVLLNTLAKKYSLPIIVSTHPRTRKKIDSLDLKFSPLVQLMKPLGFSDYV
                     KLQQHAKVVLSDSGTITEESSILNFPAINIREAHERPEGFEEASVMFTGMNIDRILQV
                     IAILDQQPKGEKRILRKVYDYEAPNVSEKIVRIIHSYTDYVNRVVWKKY"
     gene            5273..6475
                     /gene="wbuB"
     CDS             5273..6475
                     /gene="wbuB"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="AIG62767.1"
                     /translation="MHLALIIDDYLPDSTRVASKMFHELACAFVNGGHRVTVITPYYG
                     RGNRLKKNHLDGVEVWYFKNPRIKDVGKIKRAFAETVLSLNAWIAIKHEPEIKNFDAV
                     IYYSPSIFFWSISQKNKEKSKCYAYQILRDMFPQWSVDAGLIRAGSLIEKYFRFFEKI
                     NYSQADYIGLMSQKNVELFNSKYNFCATGVLHNWARFECISYEPKYKKCLGLNDKVIF
                     LYGGNIGHAQDMGNLLRLVRNMLPYDKAHFLFVGQGDEVELVNSLVKQWKLENLTYLP
                     PLTQSEFKVLLSEVDVGLFSLAKNHTAHNFPGKILGYMVNSIPILGSVNDGNDLMPLI
                     NDANAGMVYVNGDDEHLFQAALTLLNDKSIRNNMGKCALALLRKEFDVEGVVIEIINK
                     INGFYEKN"
     gene            6453..6866
                     /gene="wbuC"
     CDS             6453..6866
                     /gene="wbuC"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AIG62768.1"
                     /translation="MGFMKKIDSEIIDKLFNQAKDSDRKRAHYLLHQSYEEKVQRLLI
                     AFLKNSYVEPHFHKNSNQWEMFIVIQGVFELTKYSEGGSIIERIIIEAGADILAVEIY
                     PCEIHSVRCLSEKGLLMEVKEGPFNPLEAKEYLVN"
     gene            6932..8182
                     /gene="wzy"
     CDS             6932..8182
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AIG62769.1"
                     /translation="MNILDKKKNSTSFNYYKITTGLYIILTMYSLLVSIYLSIPLLLY
                     GIITNKQGTPLFIALFFSILGYSFTPPFEFDLYRHYESYEYYLQYNEFLYPIKDFYLS
                     FLFLIGKNLSLKKEFLYFISILIYYTSILTVVLKLKKNVPLTGTYFFIIFVLFILNNT
                     VVEITGLRFTTALGFISLFIYYNYIESRKKTSYFFLFLSVLSHFSVIILPIVIILLRL
                     LYKLFNSRLNAIIMIFTSVIAGLYFVEPIVAFAVIGVEKLFNIYIGAETYTSGLWGAD
                     RVTLHGFSQTGIMFENIKLGISILIPVIISITFILNKDYGKNELTKLFVACSIIFFIF
                     IPFDTVYLRYQYLLIVTALIIFATKNYKFKYLDGQFIGISMIFLKISMQITVGLLSTY
                     VFYIRGLKFDFINTNLVAVLFNIM"
     gene            8201..9472
                     /gene="wzx"
     CDS             8201..9472
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AIG62770.1"
                     /translation="MNIFKTAILSLISTIIKLLSALIINKVISIFIGPSGIALIGQFQ
                     NLIQMTNTLSQGGILSGVTKYTAEYNKHSPENLENLWGTALRIITFLSIIIGLLLFFF
                     AGTLSSYLFSTQGYSVFIQLLGCVTIFTSVNNYALAILNGLKKIKLYVLVNIYQSIIS
                     LILSILFVILYSLKGAFLALILNQSVVFITLLFVFYKNSKLINIKKIIRHMSNEEVKK
                     LFSFSLMALTSAIVGPVSLMLIRNYIQGNISINASGYWQSMWYISSMYLLVVTTTLSI
                     YYLPRLSELVSNDDLKIELIKGIKIIVPLVVAASLTVYLLRDFIISLLFTSEFLSMRN
                     LFQWQVCGDVLKITAWLFSYVLIAKAQSKIFIILEVINFSLFTALSIFMIHIYGLIGG
                     SYGYFLSNLIYLILVSIAVKIRFFKQHNANI"
     CDS             9484..10611
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="AIG62771.1"
                     /translation="MAKYIFIARNTIPLANPAGLVIYRIAKALSNLKHNVEIFSLNKP
                     SGEQQIPKWLTESDDIHVNGVNLTKKNILFSYLKFLFSLSGTIKKELKKNETIYIVTH
                     TNPLYTHWIGFICKLIFNKRIKWISSFTDPYVRSPFSGYAKFTLGTCVRILEQRLSFF
                     FSDKIVFVTDTMRDFICNGNKKALNKSIVVPFFYLSNWKEKILNYDKIKEKRNEQRLL
                     IMHAGSIYGNRDASKFIDALSDHESTIIFKNFGVVNQKIQYSSANIVITSPLPYDDMM
                     VNLKKADYILIIDSFFKDIKNPYMPSKVVDAMYLHKPIIGITESNSELDFFLRKTGNI
                     SIPNDRKIISSVLSNLKQNSKCDFSMYADLNLSDKFRELEC"
     gene            10684..11682
                     /gene="galE"
     CDS             10684..11682
                     /gene="galE"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="AIG62772.1"
                     /translation="MILVTGGLGYIGSHTVVELLSRVYEVIILDNFSNSHPGVGSKIQ
                     TISGVKPLIFEGDIRDVRLLNKIFSDNKISGVIHFAGLKSVSESIRNPLEYYSNNVAG
                     TLNLIEYVLRYRINNFIFSSSATVYGSPEKIPLDESCDIGRTTNPYGTSKYLVERILQ
                     DISKAHKELNVTILRYFNPVGAHPSGLIGESPVGVPNNLMPFISLVANGKFEYVSVYG
                     NDYDTPDGTGVRDFIHVMDLATGHVAALENQNTENNLHIYNLGAGIGYSVLDIINKFE
                     EVNGVKVNYKFCDRREGDIGECWSDPSLAFEELKWKATKTLDDMVMDAWKWQKNNS"
ORIGIN      
        1 ctggtagctg taaagccagg ggcggtagcg tgctcatttg ttataacgac ttacaacgta
       61 ttcacagtat agtttcggct gtgtaaacat ggtgtgttga tacacaaaag ttataagaat
      121 aaaggcgtca ggaatgaaaa tagcatttgt tggatataat cccgggaaaa ttggtggtat
      181 agaggctttt tcaaggaata tgaaaaacga cattttccct gagtctgaat ttgtgtatga
      241 atatgatgaa gctggtacat ttaaagttga taactgcgtg cccttattaa aacgtaatct
      301 ttttaataga gtacttaaca gattaacaaa cggaaattac acaaaaagta aaatcaaaaa
      361 aaaattaaaa gaaaaaaaat atgatattat catcttgaac tcaccaaaat acttagatgt
      421 gtcaccagat ttgtgtagaa caattttagt tcagcatacg acactagata attggtggaa
      481 atcaaaatat aatttcggga gtaatcgaaa tctggctaaa cttgttaaaa aagttggtaa
      541 ggtagtagcc ttgtctgaaa tggaaggcca ggtgataaaa aattttttta gagttgaagc
      601 taacaaaatt agggtgataa atttctcggc tggtatccct tttttatatg gcaagaaaga
      661 gcctggtaaa aaccttatta tgctagcaag atttcaaaat gaaattaaaa gaattgatct
      721 tgtgattgaa gctatggatg acttacccga ctatacgtta aatgtttatg gagatggaaa
      781 agatcgtgat tatcttatag aattatcgaa acataaaaaa aatgtaaaaa tacactctag
      841 tataacagat aaaacatcag tcctggataa aaataatata tatgttttga gtagtgaata
      901 tgaggggtat ccagtaagtg ttatcgaagc tatttcaagg aaactaccag tagtagcaag
      961 aaatacattt tactcggcgt ctgaacttgt gaaggataat ggtatcttat tggataaaac
     1021 ttggagtaag gacgagtttt gtaatgcagt ccgttcttgt tatgaaaatt atgagcaata
     1081 ttcaaataat tcagagagct atttttataa atatgatatt accacaataa aaaataattg
     1141 ggttagtttg gttcaggaac ttaactatgc gcaatgatat tgatgtggtc atcacatggg
     1201 ttgatggaaa tgaacctgat tggattaaag aatatactta ttataagggc tgtgagccgg
     1261 gtcgtttccg agatataggg gtattgaaat atatatttag aagtcttgag aaaaatatgc
     1321 catgggtgcg taaaatacat tttgttacta atggtcaata tccgacttgg ttgaacttag
     1381 aacatccaaa gatttatttt catgctcaca aagatttttt tatttacaaa gatgctcttc
     1441 cagtgtttaa ttcaaatgca attgaattaa attttgcaaa tattccagat ttatctgagc
     1501 gatttatttt atttaatgat gatatgcttg ttttaaaaca agttaaggaa gagcgttttt
     1561 ttgataatgg actgccagtc gattattgca aattatcata ccctcgcaaa ggtaggttat
     1621 ataaatggct aaaaccgcaa aattacctag catgtgaatt gattaataat aattatttat
     1681 atttaaaaaa cacaagagtg aatcaaataa ataagaaaaa cctttataat aaagattata
     1741 acctaagtac aaatatatcc aattttatat tttcaatatt tagaaaagtt aaatggtttg
     1801 aagtgtatca ccatccgcaa ccacatttaa aatcaacttg gctggataaa acatccgaat
     1861 tgcgtaatgc tgtagtcaga aatacaatat atacgaaatt tcgggcaaaa actgatgtca
     1921 gtcattattt gtatcgttat caaaatctgt tgaataataa atttacacca aggcaatata
     1981 acgatcatta ttcaatatac gtaaaaaatg ttaatgaatt taataagaat ataaaaaaat
     2041 acttttcaaa tacttttctt tgtatttgtg aggatgagaa tatgggagat acagaattta
     2101 ttaaattaag aagaatatta ataaaggaat tagagattat gttcccaaac aaatgttcat
     2161 atgaactata atacacttac gtttgaataa agataaattt gttattatcg agagtagggt
     2221 taatatgttt aaatcaaagg tgttgttaat aactggtggg actggttctt ttggtaatgc
     2281 agttttgaag cgttttcttg aaacggatat aaaagaaatt cgtatatttt ctagggacga
     2341 aaaaaaacaa gatgatatgc gcaagtttta tcgttcggaa aaactaaaat tttatattgg
     2401 tgatgtgaga gattaccaaa gcatctcaaa tgcaatgcgt ggagttgatt acgtttatca
     2461 tgccgctgcg ttgaaacaag ttccctcatg tgagttttat ccgcttgaag cagttaaaac
     2521 taatgtgtta ggtactgaaa atgttttaga agccgcaatt aatcattctg tgaaacgtgt
     2581 tgtatgttta agtacagata aagcagttta tcccataaat gcaatgggga tatccaaagc
     2641 aatgatggaa aaagtgattg tagctaaagg acgtaatctt agtgaaagta acactgttat
     2701 ttgttgcacg cgttatggca atgtaatggc gtcgcgggga tctgtaattc cattattcat
     2761 caggcaagca gttaatggtc aaccaataac cataactgat ccaaatatga cgcgttttat
     2821 gatgacgctt gatgatgcag ttgatctagt tctacatgct ttcaagaatg gtaagagtgg
     2881 ggatactttt gtgcaaaaag ctcctgctgc tacgattgat attttggtta aatcgctact
     2941 ttctttttta aataaagaag atcatcctat tactgtaatc ggttctcgcc atggcgagaa
     3001 acaatttgaa gcactatgca gtagagagga aatgttagta gcggaggatc aaggtcgcta
     3061 ttatcgcatc cctgctgata atcgtgattt aaattactca aaatattttg aagttggtga
     3121 gaaaaatctt cacttgattc aagattataa ttcaaataac acagaacaac ttgatgttat
     3181 gggaatgaaa aatcttttaa gaaagctaga ctttatgcgt gaaatagagg ctggaaacat
     3241 tgtaattcct gaaggagtat agatatgaaa gtgcttattt taggggctag cggcatgtta
     3301 gggtatagcc tctacgcaaa catgagtgaa tatcatgaat ttgaagtatt tggaacagta
     3361 cgagacatcg cagggaagga acattactac aataaaccaa aaggatacat aataaaaaat
     3421 gttaatatct tagatttaga taaagtgaaa cagatagttg ctgagttaaa acctgatgtt
     3481 gttattaatt gcattggtct aattaaacaa ctttctatat caaaaaaata tacgtcagca
     3541 ataacgatta atgcattatt gcctcatcag ttggctgaaa tatgttcctc ttatgaagca
     3601 aaactcatcc atttttcaac ggattgcgta ttcgatggca aaaaaggtaa ctataaggag
     3661 gatgatttac ctacagccac agatctatat ggtaaaacaa aacatatggg tgaggtttat
     3721 tatggagggc acattacact aagaacatct ataattgggc atgaattgat atcttctata
     3781 agtttagttg agtggttcct gaatcaagaa aaagaagtta agggtttcac taaagctata
     3841 ttttcaggac tacctacagc gtatattgca aagttgctag ctgaaaaaat catccctaat
     3901 aaatccttaa gtggattata tcatttatcc acattcccga ttaataagta tgacctatta
     3961 acaaaaatat caaaagtata tgataaaaat ataaatattt ataagtctga tgaggtaatc
     4021 attgatagaa gtttaaattc tgaaaaatta agaagcgaaa ttggttttgt tccacctgat
     4081 tgggatgaac ttattgaata catgaatgct gattttaaaa aatggtacac gttatgcaga
     4141 tgaataaaaa aatgaaagtt gctactattg tcggaactcg tcctgaaatc atcagattat
     4201 caagaataat atctagacta gatgagtgca ctgaacatac gcttatccat accggacaaa
     4261 actatgatta tgaattgaac caaatatttt ttaatgattt gaatatacga gctcctaata
     4321 tttttcttga atgcgcaggc gatactcccg cagaaacaat cgctaatgta ataagcaaat
     4381 cagatcaatt atttgaaaaa ttgaaacctg aagcacttct tatattaggg gataccaata
     4441 gtgcattatc tgctatatca gccaaaagaa gaaaaattcc tatttttcat atggaagctg
     4501 gaaatagatg ttttgattta agggttcctg aggaaatcaa cagaaaaata gtagaccata
     4561 ttgctgacat caatttgcca tatagctcaa ttgctagaga acacctttta cgtgaaggtt
     4621 taaatccaga tcttgtaatc aaaacaggaa gtccaatgga tgaagtgttg aacttttatg
     4681 cttctaaaat tgatgcgtct ttatctttac aaaagatgaa tgtcgaagaa aataaatatt
     4741 tccttgtaag cgtgcaccgt gaggaaaacg ttgattctga cgtaaacatt aataattttg
     4801 ttgtattatt aaatacttta gccaagaaat attcattacc tataatagtg tctactcatc
     4861 ctagaacgcg taaaaaaatt gattctttag atcttaagtt ttcaccttta gttcagttaa
     4921 tgaagcctct tggatttagt gattacgtta aactacaaca acatgccaaa gttgtcttga
     4981 gtgatagtgg aacgattact gaagaatcat ctattttgaa ttttcccgcg attaatattc
     5041 gtgaagccca tgaaagaccc gaaggatttg aagaggcatc tgtaatgttt acaggtatga
     5101 atattgatag aatccttcag gttattgcta tattagatca acaaccaaaa ggtgaaaaac
     5161 ggatcttacg taaagtttat gattatgagg ctcctaatgt ttcagaaaag atagttcgta
     5221 taatccattc ctatactgat tatgtaaatc gagttgtgtg gaaaaaatat taatgcacct
     5281 tgctcttatt atcgatgatt atttaccgga tagcacaagg gttgcatcaa aaatgttcca
     5341 tgaactggct tgtgcatttg ttaatggtgg gcatagagtt acggttataa cgccatatta
     5401 tgggagaggg aataggctaa aaaaaaatca tttggatggg gttgaagttt ggtactttaa
     5461 aaaccctaga attaaagatg ttggtaaaat taagcgagca tttgcagaga ccgttctttc
     5521 gttaaatgca tggatagcta taaagcatga acctgagata aaaaattttg atgctgttat
     5581 ttattattcg ccatcaattt ttttttggtc cattagtcag aaaaataagg agaagtcaaa
     5641 atgttatgca taccaaattc ttagagatat gtttcctcaa tggagtgtgg atgctggtct
     5701 aattagagca ggttcattaa tagaaaagta ttttcgtttc tttgagaaaa ttaattactc
     5761 gcaggccgat tacatcggcc tgatgtccca aaaaaatgtt gaactcttca atagtaagta
     5821 taatttttgc gcaacagggg tattacataa ctgggctcgg tttgaatgta tatcttatga
     5881 accaaaatac aaaaaatgtt tgggattaaa cgacaaagta atatttttat atggcggtaa
     5941 tattggccat gctcaagata tgggaaattt attgcgactg gtcaggaaca tgcttccata
     6001 tgataaagct cattttttat ttgttggaca gggggatgaa gttgaactgg ttaattcatt
     6061 agtgaaacaa tggaaattag aaaatctaac ttatctacct ccattaactc agtcagagtt
     6121 taaagtgcta ctttctgaag ttgatgttgg tctgttttcc cttgctaaaa atcatacggc
     6181 gcacaatttt ccagggaaaa tattgggcta tatggttaac tcaataccaa tattaggcag
     6241 cgtaaacgat ggtaatgatt taatgccttt aataaatgat gctaatgctg gcatggtata
     6301 cgtaaatggg gatgatgagc atctttttca ggctgcatta acattattga acgataaaag
     6361 cataagaaac aatatgggca aatgtgcatt agctcttctt cggaaggagt ttgatgtaga
     6421 aggcgttgta attgaaataa taaataagat taatgggttt tatgaaaaaa attgattctg
     6481 aaataataga taaattattc aatcaggcaa aagatagtga tagaaaaaga gcacattatc
     6541 ttctacatca atcctatgaa gaaaaagtac agcgtctgtt aatagctttt ctgaaaaata
     6601 gttatgttga gcctcatttc cataaaaaca gtaatcaatg ggagatgttt attgtaatcc
     6661 aaggtgtttt tgaattaact aaatattctg aaggaggttc tattattgag cgaataatta
     6721 ttgaagctgg cgctgatatt ttagctgttg aaatttatcc atgtgaaata catagtgtaa
     6781 gatgcctttc tgaaaagggg ttactaatgg aggttaaaga aggcccattt aatcctttag
     6841 aggcaaaaga atacttggta aattgaaaat acaaaaaaag attgaatggt gttgattgtt
     6901 aagggtgtaa ttacatatac taaataattt tatgaatata ttagacaaga agaaaaatag
     6961 cacatcgttt aattattata agataactac tggattgtat attattttga caatgtatag
     7021 tctgttggta agtatttatc ttagcatccc tttattatta tatggtataa taacaaacaa
     7081 acaaggaact cctttattta tagcattatt ttttagtatt cttggttata gttttactcc
     7141 tccatttgaa tttgacttgt atagacatta tgagtcatac gagtattatt tgcagtataa
     7201 tgagtttctt tatccaatca aagatttcta tcttagcttt ctttttttga tcgggaaaaa
     7261 tctatcctta aaaaaagaat ttttatattt tatttcaatt ttaatttact atacaagcat
     7321 attaactgtt gttttaaaat taaagaaaaa tgtacctcta actggaacat attttttcat
     7381 aattttcgtt ctttttatat taaataatac tgttgttgaa atcacaggac tccgatttac
     7441 taccgcgctg gggttcattt ctctgtttat atactataac tatattgaat caagaaaaaa
     7501 aacatcatat ttctttctat ttctttctgt attaagccat ttttctgtaa tcatacttcc
     7561 tattgttatc attttattgc ggttgttata caaattgttt aactctcggt taaatgcaat
     7621 aataatgatt tttacttcag taattgctgg tttatatttc gtcgaaccaa ttgttgcatt
     7681 cgctgttata ggtgtggaaa agttatttaa tatttatatt ggagctgaga cctatacgag
     7741 tggattatgg ggggcagatc gagtaacatt acacgggttt agtcaaacag ggataatgtt
     7801 tgaaaatatt aagttaggaa taagcatact tattcctgta attataagta taacttttat
     7861 tttgaacaaa gactatggta agaatgaatt aactaaattg tttgtcgcgt gctctattat
     7921 tttcttcatt tttattcctt tcgatacagt atatctgcgt tatcaatatc ttttgatcgt
     7981 aactgcatta ataatttttg caacaaaaaa ctataaattt aaatatttag atgggcaatt
     8041 tattggtatt tccatgattt tcttaaaaat atcaatgcaa attacagtag ggttgttatc
     8101 cacatatgtt ttttatatac gtggattaaa atttgatttt atcaatacaa accttgtggc
     8161 tgttttgttt aatattatgt aaagttatag ggaggggggg ttgaacattt ttaaaaccgc
     8221 aatacttagc ttaatatcta caataataaa attattaagt gcgttaatca taaataaagt
     8281 catttccatt ttcattgggc catctggaat tgctttaatt ggtcagtttc aaaatcttat
     8341 ccaaatgaca aatactcttt cgcagggagg tatattgtct ggagtaacga aatacaccgc
     8401 tgaatataac aagcactcac cagaaaatct cgaaaactta tggggtaccg cactaagaat
     8461 aataaccttc ctttcaatta taatcggtct tcttttgttt ttttttgctg gcacattgtc
     8521 ctcctactta tttagcacac aaggttatag tgtttttatt caattgcttg gctgtgttac
     8581 aatattcaca tcagtaaata attatgcgct tgcaattctt aatggattaa aaaaaataaa
     8641 actttatgtt ttggtgaata tatatcaaag cattatttca ctaatcttat ctattctttt
     8701 tgttatatta tatagtttaa aaggtgcctt tttggcatta atattaaacc aatctgtcgt
     8761 atttatcact ctcctgtttg ttttttacaa aaatagcaag ctaataaata taaaaaaaat
     8821 tattagacat atgtctaatg aagaagtaaa aaaactattc tcattttctt taatggcttt
     8881 gacttctgcg attgttgggc ctgtgtcatt aatgttgatt agaaattata ttcaaggcaa
     8941 tatcagtatt aatgcttctg gttattggca atctatgtgg tacatttctt caatgtactt
     9001 attagtagta actactacat tatcaatata ctatttaccc cgattgtctg agttagtcag
     9061 taacgatgat ctcaaaattg aacttataaa gggtattaag attattgtgc ctcttgttgt
     9121 agctgcctca cttacagtct atttgctaag ggatttcatt atatcattac tttttacgtc
     9181 tgagttttta agcatgagga atttattcca atggcaggtt tgtggtgatg tattgaaaat
     9241 tacagcttgg cttttctctt atgtattaat tgcaaaggct caatctaaaa tatttatcat
     9301 attagaagtg ataaacttta gtctatttac agccttgtca atatttatga ttcatattta
     9361 tggtttgata ggtggttcat atggttattt tttatccaat ttaatttatc tgatacttgt
     9421 ttctatcgct gttaaaatcc gcttttttaa gcaacataat gcgaatattt aaggaaataa
     9481 actgtggcta aatatatatt tatagcaaga aatacgattc cacttgcaaa tcccgctgga
     9541 ttagtgattt atcgtattgc aaaagccctg tctaatttaa agcataatgt cgagattttt
     9601 tccttaaata aaccatctgg agaacaacaa attcctaaat ggttgactga gagtgatgat
     9661 attcatgtga atggtgttaa tttaactaaa aaaaatatat tatttagtta tcttaagttt
     9721 cttttttcgc ttagtggtac cataaaaaaa gaactaaaga aaaatgaaac aatatatatt
     9781 gtaactcata ctaatccgct ctatactcat tggattggtt tcatatgtaa gttaattttt
     9841 aataaaagaa tcaaatggat ttcttctttt actgatccat atgttagatc tccatttagt
     9901 gggtatgcta aatttacgtt ggggacttgt gtccgaatcc ttgagcaaag gctttcattt
     9961 ttttttagtg ataaaattgt ttttgttaca gacacaatga gggattttat atgtaatgga
    10021 aacaaaaagg ctcttaataa atcaattgtt gtcccctttt tctatttaag caattggaaa
    10081 gaaaaaattc tgaactatga caaaataaaa gaaaaaagga atgaacagcg gttactgatt
    10141 atgcatgcag gttcaattta tgggaataga gatgcttcta aatttataga tgccttaagt
    10201 gaccatgaat caacaattat attcaagaat tttggagtag ttaatcaaaa aattcagtat
    10261 tctagtgcta atatagtaat tacttcccct ttaccttatg acgatatgat ggttaattta
    10321 aaaaaggctg attatatact tattattgat tcctttttta aggatataaa aaatccatat
    10381 atgccgtcta aagttgtaga cgctatgtat ctgcataaac caattatcgg aataacagag
    10441 tcaaactctg aattggattt tttcttacgt aaaacaggga atatatcaat tcctaacgat
    10501 agaaaaataa tatcgtcagt actatcaaat cttaaacaaa atagtaagtg cgactttagc
    10561 atgtatgctg atttaaatct ttcagataaa tttagggaac tggaatgttg aacaccatcc
    10621 tttttgtttc tatgctctaa taagagaaat gtttctccaa aatgacggag aggaggagta
    10681 ccaatgattt tagtaacagg tggtttaggt tatattggat ctcataccgt agttgaattg
    10741 ctatctcgtg tttatgaagt gattattttg gataacttct ctaattcaca ccctggcgta
    10801 ggatctaaaa tacagactat aagtggtgtt aagcctctga tatttgaagg cgatattagg
    10861 gatgttaggt tacttaataa aatattctct gataataaaa tttctggcgt tattcatttt
    10921 gctggcttaa aatcagtatc ggaatcaatt aggaatcctc tggagtatta cagcaacaat
    10981 gtagctggaa cattaaattt gattgaatac gtgttacgct atagaattaa taattttatt
    11041 tttagttctt cggctacagt gtatgggagt ccagaaaaaa tcccactgga cgagagctgt
    11101 gatattggta gaacaactaa cccatatggg acctctaaat atcttgttga acgaattctc
    11161 caggatatat ctaaagcaca taaagaacta aatgtcacaa tactaagata ttttaatcca
    11221 gtaggggctc atccttcagg cttaattggt gagtctcctg tcggtgttcc taacaatttg
    11281 atgccgttca tttccctcgt agcgaatggt aaatttgaat atgtctctgt gtatggaaat
    11341 gattatgaca caccggatgg aactggtgta agggacttca tccatgtaat ggatcttgct
    11401 acggggcatg ttgcggcact agagaaccag aataccgaaa ataatttgca tatctataat
    11461 ttaggtgctg gtataggata ttctgtgcta gatattataa ataaatttga ggaagttaat
    11521 ggagttaaag tgaattacaa attttgtgac agacgagaag gagatatagg tgaatgttgg
    11581 tcagatccaa gtttggcatt tgaagagcta aaatggaagg cgactaaaac cttagatgac
    11641 atggttatgg atgcttggaa atggcaaaaa aataattctt gaatatttta atctatcttt
    11701 gtagcaaaga tattttaata attatgtgta gcatttaacc aatttaccgt atcaggtaat
    11761 ttgcttatta ctcgctttaa ttcctgacag gagtaaacaa tgtcaaagca acagatcggc
    11821 gtcgtcggta tggcagtgat ggg
//

</pre>