<pre>LOCUS       KJ778800               10818 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain E43478 serotype O179:H8 O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ778800
VERSION     KJ778800.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10818)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Swimley,M.,
            Baranzoni,G.M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E43478"
                     /serotype="O179:H8"
                     /db_xref="taxon:562"
     misc_feature    1..10818
                     /note="O-antigen gene cluster"
     gene            86..1546
                     /gene="wzx"
     CDS             86..1546
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AIG62761.1"
                     /translation="MNFLVYLALSIVSKLIASVREFGVGFIWGTGSIVDAFILAMMLP
                     MFFIDIASVMFNSTYIGIYVNSANKERRFFFKDVFYFTLIYVLFTNIIVIFFLKEYIA
                     YSDIPIETKHIIDNIIIYIVVYYSLLAFCEFYKAHLMACEKQKMMPLPMICANIVFIS
                     TMYYFSEGANDIALVSCFVFSSVIQFILYIPLIIKKDSVYIMNSSVSHSREANKKLFL
                     KNLVPVFFNAAVNQSSKITDKVIASSLIIGSLSSLYFAQQLYALYVNIIIVSVLSFAY
                     PKICKKANENKYEFSSYIVQVIIVLFVLLVYPVMLGWRYAQDIFKFILVNSTTQNIKV
                     VSEMFMVLLSVVVFESISASLKRAFWALNKMKVTVYISGFTVIINIILSVLLSSYYGP
                     KGLVYSYCAATILSTMLLIFYFNKVSKITIPKNIFLPIMTASIISVTFYILLGFFRID
                     LNKPISLLIPGLIVVLGMFLLFVCFNKNSISKVLKE"
     gene            1543..2682
                     /gene="pglJ"
     CDS             1543..2682
                     /gene="pglJ"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylgalactosamine-N,
                     N'-diacetylbacillosaminyl-diphospho-undecaprenol
                     4-alpha-N-acetylgalactosaminyltransferase"
                     /protein_id="AIG62760.1"
                     /translation="MKNICFVNSYACLGGAERSLQSLIEGFIAKDYNVHVIIGEDRGP
                     LLDWVREKKIRYKIIEQPSLQKKYGRVLFPLYLLHFLILFYLYIKKHKIEIIHANTFR
                     SRIYCALVKYITSCKLICHVRDIEYSRLNKALLSIYDHTIAISDAVKSTVLKGTKGNS
                     LLRKKIITIHNGVKVCHLPQVKKKRDDNNINIGMFARFDEWKCHHILIEAANKLKKNN
                     VEMKVGKKINYYLYGEAIRESEAIYYAHIKKMVHDLQLENDVFFMGFHDEPINEMHKM
                     NIIVCPSDNEPFGRVLIEAMAIKKIVIASNNGGVKDILGSKFSDLTFEPRNAEALARK
                     IEMYAESQNEFEYLYCDLLYKEYRERFSLESLLNNIEQLYLKDIK"
     gene            2682..3785
                     /gene="wzy"
                     /locus_tag="PROKKA_00003"
     CDS             2682..3785
                     /gene="wzy"
                     /locus_tag="PROKKA_00003"
                     /inference="ab initio prediction:Prodigal:2.60"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AIG62759.1"
                     /translation="MFTISPVFFENITFQGSRTLADVLFCISLLLIICKHKKVNISLL
                     GIVYIIIVLVIMSVNAALGFSNASTTINNELFDKLTWLILDRNINEFIPFGDYYRNIL
                     IYTRYLLIPFYFWMGLYYSKLVGKERAIVTLYNGLIICLLLNMLYGIYNDDDRLTGFF
                     ENTAALSSLALLCMLLSLFTKSKYKIILSWVIGIIALLMSQTASAFIGLTAVVLILVF
                     NIRRHHKKTVYITSLLSVVIASIGLMPEIMEFIARYMYTGSLINRINTWSTIINYFDS
                     YQVAILGLGSFPLFADNIFIWLISGFGIFSFVIYMYLVSIGNKSQKSALFISLLLWQG
                     VLFPGFIMPYMLMTTFITLGILSNNKGCENECI"
     gene            3772..4323
                     /gene="cysE"
     CDS             3772..4323
                     /gene="cysE"
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="AIG62758.1"
                     /translation="MSVFSCVIKDVKRYDQKVLYGFVIALTKIGFWAVLNYRLGKLMR
                     EQIKIPILKQILWFLTGLTRLVINIITGIDIPYSANIGGGFKICHFGTIFISSKVVIG
                     DNCTIHQGVTIGKGGHNTETDEPVIGNDVFIGANATVIGAITIGSRVRIGSNVCCYKS
                     INDDMTVVANRIRVIKHENSSVT"
     gene            4301..5425
                     /gene="kanE"
     CDS             4301..5425
                     /gene="kanE"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-kanosaminyltransferase"
                     /protein_id="AIG62757.1"
                     /translation="MKIVLLHNEIKPFRLPLFNYLNSIYDVKIYCLRSNKLTENNIKD
                     VEYGRYIRIPKMQDLEIPLDLWFFLKNEKPDVIISTDLGYAITYIGYLYSRVNQCKFV
                     LWNEQWSDILHPRRYLTKPLERLICRKADKILSFGYKHAEYLIQLGAKENNISIVPNA
                     VPDFYPLSKSHNENNEFFYIPWIHDVTKIKIVCPGRLVKFKGHETILNSAVGIIKANK
                     NARFIFAGSGPEFGQLQQMVNKLGLNDYVFITGVTFNEEMKNALLINADLVILASVKS
                     KKIRAVEAWGLVVNEAIQYKKKIIVSDATGVANELIIDNETGGVFENGNHIDLQNKIL
                     SCINHPEQWNIYAEKAYEKLITDYSMDVLIKKITFALEEI"
     gene            5428..6540
                     /gene="wfaU"
     CDS             5428..6540
                     /gene="wfaU"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferases group 1"
                     /protein_id="AIG62756.1"
                     /translation="MKVFLISINSDKENTGGGIYLRTLKTLYERNGYKIHIFSKDCDL
                     YKVKKNILTDLVGRLLLCPSYIGYYIFHILIFSRNFNTIAIHSTRLGIIARVIKLIHP
                     EKCIIVHSDNVESALIRELRSKKSLAKTILMFIDKLLMPLSEKYCGKYSDKMTFITEE
                     DKSQSLKYNLIPTSNKYITLPVLLKKQKLKELEKDNSVLFTGSFDFYPNQHAFMRLIN
                     LANANVNTKFVAAGRYLSKYIERGQIKLPSNMFIFSDISSDDMELLYRKAILFICPVS
                     YGSGMKTKIAEALSYDLYVIADERSTFGYQAAVTNRVLYKQSSDFFDFCEIGQLEKII
                     ENLKNTSNSCSITPYVVFEKYYSIDSGMNIFGRLLQ"
     CDS             6537..7730
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferases group 1"
                     /protein_id="AIG62755.1"
                     /translation="MKKILVLTPRFPFPVIGGDRLRIYKICQELSKHYELTLLSLCET
                     RKELTMEVDDKVFKNIYRVYLSRLQSKLNVISAIPTRKPLQIAYYSSWKFKSKVNELM
                     PYHDAVFSHLIRTGDYVKNINIKSILEMTDAISLNYKRVAESNSEFSLRKLVYIFERK
                     RLEKYERNIVNSFDMVSLISGIDKDYLYPQGHHKVKVYGNGVDTKNLIYKKRTVNNTE
                     FISIIFIGNMYSLQNMDAVKWFCHEILPNLCDATYKFKLKVVGKIKDSDRDYLNTIPN
                     VEVTGTVDDINEACSDGHLAICPIRLGAGIQNKILEYMALGLPCITSSVGFEGIGATD
                     GREILIANNLEEYQRKIHELINNKELFDKVSSNARTFVETHFSWEARLNPLINDLDFL
                     IGNSI"
     gene            7727..9121
                     /gene="manC"
     CDS             7727..9121
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase RfbM"
                     /protein_id="AIG62754.1"
                     /translation="MILPVIMAGGTGSRLWPLSRTHFPKQFHKLINEQSLLINTITRL
                     KGFSHLPPLIISNEDHRFLVAEQVRQIRADVSGIILEPVGKNTCPAITLAALHAIQNG
                     DDPYLLILAADHSIGNESEFNISVNNAVKYASADYLVTFGVIPTSPETGYGYIEKGKK
                     IEKNGFFVKSFTEKPDYQTAHSYITSKNYLWNSGMFMFKASVFLQELKTFEPEILDIC
                     GKAIQNSNKDLDFIRIDSELFSKCKSESIDYAIMEKTDKAVVVPMDAQWNDVGSWSAL
                     WDLAEKDNDNNHISGDVVIKNSTGNYIRSESGLVATIGINNLIIVNTKDALLIADRAD
                     AQNIKDIIKKLKTVERRELDYFTNEFRSWGMIEKIDYSTNHVANRITINPGKKISQQI
                     HYHRNEHWIVVSGTALINIDGEDFLLSENESTFIKIGQEHSIMNPGIVPLIIIEIQTG
                     KFISEKDVIRVRNN"
     gene            9124..10560
                     /gene="manB"
     CDS             9124..10560
                     /gene="manB"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="AIG62753.1"
                     /translation="MINNYLICNEIITTNRISFGTSGARGLVIDFSHDVCAAFTHAFL
                     SVIDDKYNFNKVALAIDNRPSSYEIAQACAFAIKQHGFNVEYHGVIPTPALAHYSMQK
                     NIPCIMVTGSHIPFDRNGLKFYRPDGEITKEDELAIINSEYTFSPVGVLPHLETSTQG
                     ADYYLERYVSLFNPEILKGKRIGVYEHSSAGRDLYAPLFNQMGAEVISLGRSDEFVPI
                     DTEAVSDEDRILAREWSKKYNLDAIFSTDGDGDRPLVADENGEWLRGDILGLLTAIEL
                     NIKALAIPVSCNTAIEESNKFASIQRTKIGSPYVIAAFADLAKQFDSVAGFEANGGFL
                     LASDLQINGKELNSLPTRDAVLPALMLLIASRNSTISQLINNLPQRFTWSDRVKNFPS
                     DSSQQIIKNAISSPNNFFNSLGYESLFCSAIDETDGARFTLNNGDIIHLRPSGNAPEL
                     RCYAEASDENQAKQYVTNVLGNIISLIS"
ORIGIN      
        1 attgagaagg tacgcagtgt actggtagct ttcaagccag gggcggtagc gtgctttttt
       61 atcaagttag gttgtaatac catcaatgaa ctttcttgtc tacttggctt tgtcaattgt
      121 aagtaaacta attgcatctg tacgggaatt cggtgtcggt ttcatttggg ggacgggaag
      181 tattgttgat gcattcattc tggcaatgat gcttccaatg ttttttattg atatcgcaag
      241 tgtcatgttt aactcgacat atattggtat atatgttaat agcgccaata aagaacgtag
      301 attttttttt aaagatgttt tctattttac tttaatatat gttttgttta caaatataat
      361 agtgatattt ttcttaaaag aatatatagc ttattccgac ataccaattg agacaaaaca
      421 tataattgat aatattataa tatatatcgt tgtgtattat tctttattgg ctttctgtga
      481 gttttacaaa gcacacttga tggcgtgtga gaaacaaaaa atgatgccat tgccgatgat
      541 atgcgcaaat attgttttta tatcaacaat gtactatttt tcagagggtg caaatgatat
      601 agcattagta tcatgctttg tattttcttc tgttattcag tttatactat atatacctct
      661 aattataaaa aaagatagtg tgtatataat gaattcatct gtaagccata gtagagaagc
      721 taataaaaaa ttgtttttaa aaaatttggt gccagttttt tttaatgctg cagtgaatca
      781 gtcatctaaa ataacagata aagtaatagc atcttcatta attatcggga gtttatcatc
      841 gctatacttt gcacagcagt tatatgcact atatgtaaat attattattg taagtgtatt
      901 atcctttgca tatcccaaga tatgtaaaaa ggccaatgag aataaatatg aattcagctc
      961 ttatattgta caggtgatta tagttttatt tgttttgtta gtgtatccag ttatgctggg
     1021 gtggaggtat gcacaggata tattcaagtt tatacttgtg aattctacta cgcagaacat
     1081 aaaagtagta agtgaaatgt ttatggttct tttaagtgtg gttgtttttg aatcaatatc
     1141 agcttctcta aaaagagcat tctgggcatt aaataaaatg aaagtcacag tgtatataag
     1201 cggatttact gttattatca atattatctt aagcgtattg ctttcaagtt attatggccc
     1261 aaaagggttg gtttattcat attgtgctgc aacaatatta tcaacaatgc tattaatatt
     1321 ttattttaat aaggttagca aaataacaat ccctaagaat attttcttgc cgataatgac
     1381 agcctctatt atatccgtta ccttttatat attattgggt ttctttagaa tagacttaaa
     1441 taaaccaatt tctttattaa ttcctggatt gatagttgtc ttaggaatgt ttttattatt
     1501 tgtttgcttc aataaaaata gtatcagtaa ggtgcttaaa gaatgaagaa tatatgtttc
     1561 gtaaatagtt atgcatgtct ggggggagca gaaagaagtt tgcagtctct tattgaagga
     1621 tttatagcca aagattataa tgttcacgtt attataggtg aggatcgagg gccacttctt
     1681 gattgggtta gggaaaagaa aatacgatat aaaataattg aacagccttc gctacagaaa
     1741 aaatatggaa gggtattgtt cccattgtac ttattgcact ttttaatttt gttctatctt
     1801 tatataaaaa aacacaaaat cgaaataatt catgcaaaca catttcgttc aagaatatat
     1861 tgtgcattgg tgaaatacat tacttcatgc aaattgatct gccatgtaag agatattgag
     1921 tattcaagat taaataaagc gctgttaagt atatatgatc atacaatagc aatatccgat
     1981 gctgttaaaa gcacggtttt gaaaggaaca aaaggaaaca gtttattacg aaaaaaaatc
     2041 attactatac ataatggtgt taaggtatgc catctccctc aagttaaaaa gaaaagagac
     2101 gataataata taaacatagg gatgtttgca cggttcgatg aatggaagtg tcatcatata
     2161 cttattgagg ctgcaaacaa actaaaaaaa aataatgtag aaatgaaggt aggaaaaaaa
     2221 ataaattatt atctctatgg agaagcgatt cgagagagcg aggctattta ttatgctcat
     2281 ataaaaaaaa tggttcatga tttacagtta gaaaatgatg tgttctttat gggttttcat
     2341 gatgaaccta taaatgaaat gcacaaaatg aatattatag tttgtccttc agataatgaa
     2401 ccatttgggc gtgtattaat tgaggcaatg gcaattaaga aaattgtaat tgcttcaaac
     2461 aacggcggag ttaaagatat actaggtagc aaattctcag atttaacttt tgaacctaga
     2521 aatgctgaag ctctagctag gaaaattgaa atgtacgcag aaagtcaaaa tgagtttgag
     2581 tatttatact gtgatttgct ttataaggag tatagagaaa ggttttcgtt agagtcactt
     2641 ttgaataata ttgaacagct atatttaaaa gatataaaat aatgtttact atatcacctg
     2701 tattttttga aaatattaca tttcaaggct ctaggactct tgcggatgtt cttttttgca
     2761 taagtttatt gttgattata tgtaaacata aaaaggtcaa cataagtcta ttaggtatag
     2821 tttatataat tattgtccta gtaataatgt cagtgaatgc tgcgttagga ttttctaatg
     2881 caagcacaac aattaataac gaactctttg ataaactcac atggttaata ttagatagaa
     2941 atataaatga atttatccct tttggtgact attataggaa tatattgata tatactcgat
     3001 atttactaat tccattttat ttctggatgg gactgtacta ttcaaaattg gtaggtaagg
     3061 aaagagcaat tgtcacttta tataacgggc tgattatttg tctcttgtta aatatgctgt
     3121 atggtatata taatgatgat gatagactaa caggtttttt tgaaaatact gcagcgcttt
     3181 cttcattagc attattgtgt atgctacttt ctctcttcac taagtccaaa tataaaataa
     3241 tactatcttg ggttattggc attatcgcat tattaatgtc tcaaactgca agtgctttta
     3301 ttggtcttac agcagtagtt ttaattttag tatttaacat tcgaagacat cataaaaaga
     3361 ctgtatatat cacatcactg ttgagtgttg ttattgcatc gattggctta atgccagaaa
     3421 ttatggaatt tattgctcga tatatgtata caggcagttt aataaataga ataaatacat
     3481 ggagtactat tattaattat ttcgattcct atcaagttgc gatactagga cttggttctt
     3541 ttcctttatt tgcagataat atatttatct ggttaatatc tggttttggg atattttcat
     3601 ttgtaatata tatgtatcta gtaagtatcg gaaataaaag tcaaaaatca gcattattca
     3661 tatcattatt attatggcaa ggggtcttgt ttccaggatt tattatgcct tacatgttga
     3721 tgacaacatt tataacactg ggaattttaa gtaataataa gggttgtgaa aatgagtgta
     3781 tttagttgtg ttataaagga tgttaaaaga tacgatcaaa aggtactgta tggatttgtt
     3841 attgcactta caaaaatagg tttctgggct gttcttaatt acaggttagg aaagttaatg
     3901 agagaacaga taaaaatacc tatattaaaa caaatattgt ggtttttaac cggattaaca
     3961 agattggtca ttaatataat aacgggtatt gatattcctt attcagctaa tattggggga
     4021 ggatttaaaa tttgtcattt tggtacaatc tttatatcct ctaaagttgt aattggcgat
     4081 aattgtacga tacaccaagg agttactata ggaaaaggcg gtcataatac tgaaacggat
     4141 gaacctgtta ttggtaatga tgtttttatt ggagctaatg caactgttat tggagcaata
     4201 actattggtt caagagttag aataggaagc aatgtttgct gttataaaag cataaacgat
     4261 gatatgacag ttgtagcaaa tcgaataaga gttattaagc atgaaaatag ttctgttaca
     4321 taatgagatc aaacctttcc gattaccttt atttaattac ttaaatagta tatatgatgt
     4381 taaaatctat tgcttacgaa gtaataaatt aacggaaaat aatataaagg acgtagaata
     4441 cggacgttat attaggatac ccaaaatgca agatcttgaa attccacttg atctttggtt
     4501 ttttttgaaa aatgaaaagc cagatgttat tatttctact gatcttggtt atgcaataac
     4561 atatattgga tatttatact ctagagttaa tcaatgtaaa tttgttttat ggaacgagca
     4621 atggagtgat atactacatc ctaggcgata tctgacaaaa ccacttgaaa gattaatttg
     4681 cagaaaagcc gacaaaatat tgtcttttgg gtataagcat gccgaatacc tgattcaatt
     4741 aggtgctaaa gaaaataata tatctatagt gccaaatgca gtccctgatt tttacccttt
     4801 atcaaaaagt cataatgaga ataatgaatt tttttatatt ccatggattc atgatgtaac
     4861 aaaaataaaa atcgtctgcc ccggccgttt agtaaaattt aaagggcatg agactatatt
     4921 aaattcagca gttggtatta taaaagcaaa taaaaatgct agatttattt ttgcaggatc
     4981 aggccctgaa tttggacagt tacaacagat ggtaaataaa ttggggttga atgattatgt
     5041 gtttattacg ggagtaacat ttaatgagga aatgaaaaat gctcttttaa taaacgctga
     5101 tttagtaatt ttagcgtctg taaaatcaaa aaaaataaga gcagttgaag cgtggggatt
     5161 ggttgttaat gaagctattc aatataagaa aaagataata gttagtgatg ctactggagt
     5221 ggctaacgaa ttaattattg ataatgaaac tggaggtgta tttgaaaatg ggaatcatat
     5281 agatctacaa aataaaatcc tttcatgtat taatcatcct gaacaatgga atatatacgc
     5341 tgaaaaagcc tatgagaaat taattactga ttattcaatg gatgttttaa taaaaaaaat
     5401 cacgttcgct ttagaggaaa tttaatcatg aaagtgtttc taatatcaat aaattcggac
     5461 aaagaaaaca caggtggtgg tatatacctc cgtacactga aaacgcttta cgaacggaat
     5521 ggttataaaa ttcatatttt ctcgaaagat tgtgatttat ataaagtaaa aaaaaatata
     5581 ctaactgatt tagttgggcg attgctatta tgtccatcat acataggtta ttatatattt
     5641 catattttaa tctttagcag aaatttcaat acaatagcaa ttcatagcac aagactaggg
     5701 attatcgcga gagttatcaa actgattcat ccagagaaat gtataatagt acattctgat
     5761 aatgttgaat cagcattaat tcgagaatta cgttcaaaaa aatcattagc aaaaacgata
     5821 ctgatgttca tcgataaatt attaatgcct ctttctgaaa agtattgtgg aaaatattct
     5881 gacaaaatga cttttataac tgaagaagat aaaagtcaaa gtctaaaata caatttaatt
     5941 cccactagta ataaatatat tactttacct gttttgttaa agaaacaaaa attaaaagaa
     6001 cttgaaaaag acaattcagt tctatttacg ggtagttttg atttttatcc aaatcagcat
     6061 gcatttatga gattaataaa tctagcaaat gctaatgtca atactaaatt tgttgctgct
     6121 ggtagatatt tatctaagta tattgagaga ggacaaataa aattaccatc aaatatgttt
     6181 attttttcag atatctcctc agatgacatg gagttacttt atagaaaagc tatattattt
     6241 atatgccctg taagttatgg gagtggcatg aaaactaaaa ttgccgaagc gcttagttat
     6301 gatctttatg tcatagctga tgaaagaagt acttttggct atcaggcggc agttacaaac
     6361 agggttcttt ataagcagtc aagtgatttt tttgattttt gcgaaatagg tcagcttgaa
     6421 aaaataattg aaaatttgaa aaatacatct aattcgtgct caattacacc ctatgtcgtt
     6481 tttgaaaaat attatagtat tgattctggt atgaacattt ttggtagatt gctacaatga
     6541 aaaaaatatt agttttaact cctcgttttc catttcctgt aattggcggt gatcgattac
     6601 gaatttataa aatatgtcaa gaactatcta aacattatga gttgactcta ttgtcattat
     6661 gtgagacaag aaaagagtta actatggaag ttgatgataa agtattcaag aatatttatc
     6721 gggtttattt atcacgactt caatcaaaat taaatgttat atctgcaatt ccgacaagaa
     6781 aacctttaca aatagcttat tattcatctt ggaaattcaa aagtaaagtt aatgaattaa
     6841 tgccatatca tgatgcagta ttctctcatt taatcagaac tggagattat gttaaaaata
     6901 ttaatataaa atcaatactc gaaatgacag atgcaatatc tcttaattat aaaagagttg
     6961 ctgagtcaaa tagtgagttt agtttgagaa agttagtgta tatatttgaa cgaaagagat
     7021 tggagaaata cgagcgtaat attgttaata gttttgatat ggtttcgtta atttcaggta
     7081 tagataaaga ttatttatac ccacaaggcc atcataaagt taaagtttat ggtaatgggg
     7141 ttgacacaaa aaatttaatc tataaaaaaa ggacggtcaa caatactgag tttatttcga
     7201 tcatttttat tggaaatatg tattcattac agaatatgga tgctgttaaa tggttttgtc
     7261 atgaaatttt accaaattta tgcgatgcta cttataagtt taagctaaaa gttgttggta
     7321 aaattaaaga ttcagaccgt gattatttaa acactatccc taatgtcgaa gtgactggaa
     7381 ctgttgatga tatcaatgaa gcatgtagcg atgggcatct tgcaatttgc cctatacgtt
     7441 taggtgctgg tatccagaac aaaattctcg aatatatggc acttggttta ccatgtataa
     7501 catcatcagt ggggtttgaa gggatcggcg ctactgacgg cagggagatt cttatagcga
     7561 ataacttaga agaatatcaa cgaaagattc atgagcttat taataataaa gaattatttg
     7621 ataaagtctc gtctaatgct cgcacttttg ttgaaacaca tttttcttgg gaggctagac
     7681 tcaatccatt aattaatgac ttagatttct taataggaaa ttcaatatga ttttaccagt
     7741 aattatggcc ggtggtacgg ggtccagact ttggccatta tctcggactc atttccctaa
     7801 acaatttcat aaattaatta acgaacagtc cttattaatt aatacaatta cacggcttaa
     7861 gggattttcc catttaccac cactgataat ttcgaatgaa gatcaccggt tcttggttgc
     7921 cgagcaagtg aggcaaatac gtgcggatgt tagtggaatc attttggagc ctgtcggaaa
     7981 aaacacctgt ccggcaatta ctctagcggc gcttcatgct attcaaaatg gcgatgatcc
     8041 ttatctttta attctcgctg cagatcattc gatcggtaat gaaagtgaat ttaatatttc
     8101 agtcaataat gctgtaaagt atgcttctgc agattatcta gtaacatttg gtgttatccc
     8161 aacaagtcct gaaactggat atggatatat tgagaaagga aaaaaaattg aaaaaaatgg
     8221 ttttttcgtt aagagtttta cggaaaagcc agattatcaa acagcccatt cctacataac
     8281 atcaaaaaat tatttatgga atagcggtat gtttatgttt aaagcctctg tattccttca
     8341 ggaattaaaa actttcgaac cagagatatt ggatatttgt ggtaaagcaa ttcaaaactc
     8401 aaataaagat ttggatttta ttcgtattga ttctgaactt tttagtaaat gtaaatcaga
     8461 atcgattgat tatgctatta tggaaaaaac tgataaagca gttgttgttc ctatggatgc
     8521 gcaatggaat gatgttggtt cgtggtctgc actttgggac ttagctgaaa aagataacga
     8581 taataatcat atcagtggtg atgtcgtaat taaaaattcc acaggtaatt atatccgctc
     8641 agaatcagga ttagttgcaa ctatcggtat aaataactta attattgtca atactaaaga
     8701 cgcattgttg attgctgatc gtgctgatgc tcaaaacata aaggacataa tcaaaaaact
     8761 caaaactgtt gagcggcgtg agcttgatta ttttacaaat gaatttcgtt catggggtat
     8821 gattgaaaaa attgattaca gtactaatca tgtagcaaat agaataacga tcaatccagg
     8881 taagaaaatt tctcaacaga ttcattatca tagaaatgag cactggatag ttgtctctgg
     8941 aaccgcttta attaatattg atggtgaaga ttttttatta tctgaaaatg agtcaacatt
     9001 catcaaaatt ggacaggaac attcgatcat gaatcctggg atagttcctt tgattattat
     9061 tgaaattcaa acaggaaaat ttatatcaga gaaggatgtt attagagtaa ggaacaattg
     9121 aaagtgataa acaactatct aatttgtaat gaaataatta caactaatcg catttcgttc
     9181 ggaactagtg gcgctcgcgg tcttgttatt gatttttccc atgatgtttg tgctgcgttc
     9241 actcatgcgt ttctttctgt tattgatgac aaatacaatt ttaataaagt tgccttagca
     9301 attgataacc gcccaagtag ttacgaaatc gctcaggcat gcgccttcgc tatcaaacaa
     9361 catgggttca atgtcgaata tcatggtgta attccgactc ctgcattagc tcattattcg
     9421 atgcagaaaa acattccctg cataatggtc acagggagtc atatcccttt tgatcgtaat
     9481 ggcttaaaat tctacagacc tgatggtgaa atcactaaag aggatgagct cgcaattata
     9541 aatagtgagt atacattttc gcctgtaggt gtattacctc atcttgaaac aagcactcaa
     9601 ggtgcagact actacctcga gcgttatgtt tctcttttta atcccgagat tttaaagggg
     9661 aaaagaatag gggtatatga acattctagt gcgggacgcg atttatatgc tcctcttttt
     9721 aatcaaatgg gtgcagaggt catttccctc ggtaggagtg atgagttcgt tccgattgat
     9781 acggaagcag taagcgatga agatcgtata cttgcaagag agtggtctaa aaaatataat
     9841 cttgatgcta ttttctcaac agatggcgat ggtgatcgtc ctttagttgc cgatgaaaat
     9901 ggtgaatggt taagaggcga tattctggga ctacttactg ctattgaact taatatcaag
     9961 gcgttggcta ttccagtgag ttgtaataca gcaattgaag agtctaataa atttgcaagt
    10021 atacaacgaa cgaaaatagg ttctccttat gtaattgcag catttgcaga tcttgctaag
    10081 caatttgatt cagtcgctgg ttttgaagct aatggtggtt ttctccttgc ctccgattta
    10141 caaattaatg gcaaggaatt aaattcgtta cctacacgag atgcagtatt accagcatta
    10201 atgctcttaa tagcttctcg caatagtact atttctcaac tgattaataa tcttcctcag
    10261 cgattcactt ggtcagatag agttaaaaac ttcccttcag attcaagtca acaaattata
    10321 aaaaatgcca tatcgtcacc caataatttc tttaatagtt taggttatga atcattattc
    10381 tgttccgcta ttgatgaaac ggatggtgca agatttactt taaataatgg tgatattata
    10441 catctccgtc cttccggtaa tgctccagaa ctccgttgtt atgctgaggc cagtgatgaa
    10501 aatcaggcta agcaatatgt tacgaatgtg ctgggaaata ttatctcttt gatttcttga
    10561 tgttataggt ttatctacgc ttatatgtgt gcgtaggttt gattacacgt agatgccggt
    10621 atacagaatt gaagaacggt atttgttgca ttaatgaaat tcagcactac acacattcgt
    10681 gcaacttgag ataacatctc aatcatattc aagtcgcgca tacatcgcgg tgaacacccc
    10741 ctgacaggag taaacaatgt caaagcaaca gatcggcgtc gtcggtatgg cagtgatggg
    10801 gcgcaacctt gcgctcaa
//

</pre>