<pre>LOCUS       DQ008593               13198 bp    DNA     linear   BCT 07-OCT-2005
DEFINITION  Escherichia coli serogroup O177 O antigen gene cluster, complete
            sequence.
ACCESSION   DQ008593
VERSION     DQ008593.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13198)
  AUTHORS   Beutin,L., Kong,Q., Feng,L., Wang,Q., Krause,G., Leomil,L., Jin,Q.
            and Wang,L.
  TITLE     Development of PCR assays targeting the genes involved in synthesis
            and assembly of the new Escherichia coli O 174 and O 177 O antigens
  JOURNAL   J. Clin. Microbiol. 43 (10), 5143-5149 (2005)
   PUBMED   16207976
REFERENCE   2  (bases 1 to 13198)
  AUTHORS   Beutin,L., Kong,Q., Feng,L., Wang,Q., Krause,G., Leomil,L., Jin,Q.
            and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2005) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P.R. China
FEATURES             Location/Qualifiers
     source          1..13198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O177"
     misc_feature    1..13198
                     /note="O antigen gene cluster"
     gene            108..1193
                     /gene="rmlB"
     CDS             108..1193
                     /gene="rmlB"
                     /note="dTDP-D-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="AAY28252.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDATAMARIFALHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVENVKSGAYQSWIAQNYEGRQ"
     gene            1193..2092
                     /gene="rmlD"
     CDS             1193..2092
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="AAY28253.1"
                     /translation="MNILLFGKTGHVGWELQRALAPLGNLIALDVHSTDYCGDFSNPD
                     GVAETIRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFSGNGDMPWLETDTTAPLNVYGETKLAGEKALQEYCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPDVAGLYHLVAS
                     GTTTWYDYAALVFEEARKAGIPLSLNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            2150..3040
                     /gene="rmlA"
     CDS             2150..3040
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="AAY28254.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQASPDGLAQAFIIGEEFIGG
                     DDCALILGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIGEKELYALAQPL
                     LKNNYGKYLTGILKNNTYVN"
     gene            3030..4295
                     /gene="wzx"
     CDS             3030..4295
                     /gene="wzx"
                     /note="O unit flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="AAY28255.1"
                     /translation="MLTKYTLIRNSILNLSGYIIPTLVAIPALGYMARELGPELFGVY
                     TLAMALVGYASVFDFGLTRAIIREIAIYRDDIVEKRKIISTSTVFLTVIGFVVTELIF
                     INVNTIVTFINVSKNNFEDANIALKILSLSIPLLLLNQLWVSVFEGEEKFGLINIQKT
                     ISNTCIVALPAVSILIESSLTYAVSGLVVGRVISLILSFLFLRKEIVASGIRFHTIVM
                     KRLIMFGSWMTFSNIISPMMVYFDRFIISNILGAKNVGFYTAPAEIISRMSIIPTSVS
                     RALFPRLSNMSDFKKFKCELLFSYAIMISICVPIVLFLLLFSGGIMYYWLGPQYYLKS
                     SSVFSVLLIGFFFNSLAQLPFSAIQSLGNSKITALLHGFEIIPYLIILYILTSHFGIV
                     GTAYAWTLRVMFDFIALYFLSSWLIKKKH"
     gene            4308..4874
                     /gene="rmlC"
     CDS             4308..4874
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="AAY28256.1"
                     /translation="MKIANTKLDGLLVIEPTVYEDERGFFYESYNEKKFMETIGASIK
                     FVQDNHSKSSKGVLRGLHFQKNPYEQAKLVRCIRGEVYDVAVDIRKDSPTFGQWFGVN
                     LSDENKKQLWIPEGFAHGFITLSDVAEFVYKTTNYYAPASEYCLKWDDKDLNIQWPLK
                     NALIVSSKDQKGKAFADFIIESNEEQRW"
     gene            4855..6141
                     /gene="wzy"
     CDS             4855..6141
                     /gene="wzy"
                     /note="O unit polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="AAY28257.1"
                     /translation="MKNKGGNVSLIQRVSSLLIALTIYLFLFDVILLGSGAWSINVTG
                     ISIRKVEYLTILLMVICTINNIKGYIYFCCATTFCLIFGVVVPFLNDVKLEYAITELL
                     SFYGILLCPLIAQNRYISVNWKKIRKFILFICIISAFIHILIWLLGLMGSGYVDLIKQ
                     LCIRVLTANNDDLIDNIIIADTPDGLFRVLLPNSSLLIIGFYLSFQQYLNRKNYIHLL
                     IVFVMLLALYTTWTRALYLSPIIIIGGVLFYKIFPFTLQLGKIGAYNLLSFIILFFIV
                     ISTVLVQPTLLSLLGIASESSDGIRYTQVIWIFNTFMNSPVLGTGLGGSAEDVRSLIA
                     PWTYEMSLLALIMKLGLVGVFLFISISAIKIPEFLEGFFDGRRIPVKKVVWIYFSLSV
                     LMMFSTNPFMLSFPGVTITLFILCELNSFKIEKGNI"
     gene            6138..7010
                     /gene="wbdY"
     CDS             6138..7010
                     /gene="wbdY"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbdY"
                     /protein_id="AAY28258.1"
                     /translation="MIQRAVIIVCYHPVKNKLENLVDKVSGPGTIVYIANNGGITAEL
                     NYTLNQKKAIVINFDRNLGLGEAINSIAEIIPESVEAIFTFDQDTSPPDDYIIKTWTH
                     FRKLMSEGINLGVLTPKFIDSRSGYLYQQKPKGYYNRYTELLVTLQSGMCIPKNVWKE
                     EKFNSDLFIEFVDTEWCYRIHSKKYKVIQMDDIIMSHEVSELSPKKILSFSLMKYKPI
                     RRYYFFRNAMFLLQQNYVPLYSKIRLLTGMCNRILSVALMDESKLDSYIHCIRGIKDG
                     VKLGKNEKNINDMH"
     gene            6982..8169
                     /gene="wbdZ"
     CDS             6982..8169
                     /gene="wbdZ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbdZ"
                     /protein_id="AAY28259.1"
                     /translation="MRKILMICTKYPLDLNSTWLTRELAEEFNNRGYVIDVICIDWGV
                     LQKRKKISLNNINIYNVPALGCKKTSFLNFFIKWGGSSLYAALLNFRVFFKRHDLVIS
                     FSPCASSWFAIILGIFFSKKSLLIYWDFFPIHQVQIDLIKGRFKTKILKCFEKALVKM
                     FDYIGCMSKGNCEFFVEYFKTRREKVFELPIWSEGLRINGLIKSKSVSFIDSNSIYFV
                     FGGQIDYGRRIECILSAAKIAHKKNNKIKTIIIGRGRLVDQVIKDAESIENGIIYSDF
                     IPRNDYLTLVSVCRAGLIATVPNVSVPTYPSKCLDYMKLSIPIIASIEDTTDFGEIII
                     NAGAGLCCNAGDSNALAAAMLSLAEDELLASQMGEKANLFFKNRHEVKTVVNNLLEHI
                     KED"
     gene            8174..9208
                     /gene="fnlA"
     CDS             8174..9208
                     /gene="fnlA"
                     /note="L-fucosamine synthetase"
                     /codon_start=1
                     /transl_table=11
                     /product="FnlA"
                     /protein_id="AAY28260.1"
                     /translation="MFKDKTLLITGGTGSFGNAVLRRFLDTDIAEIRIFSRDEKKQDD
                     MRKKYNSDKLRFYIGDVRDFSSIRNASRGVDFIYHAAALKQVPSCEFHPMEAVKTNVL
                     GTENVLEAAISNHVRKVVCLSTDKAVYPINAMGISKAMMEKVMVAKSRNVDSSKTVIC
                     GTRYGNVMASRGSVIPLFVDLIKSGKPLTITDPNMTRFMMTLEDAVDLVLYAFEHGNN
                     GDIFVQKAPAATIQTLAIALKELLNVHEHPVNVIGTRHGEKLYEALLSREEMIAAEDM
                     GDYYRVPPDLRDLNYGKYVEHGDRRISEVEDYNSHNTERLDVEGMKKLLLKLPFIRAL
                     RSGEDYELDS"
     gene            9192..10316
                     /gene="fnlB"
     CDS             9192..10316
                     /gene="fnlB"
                     /note="L-fucosamine synthetase"
                     /codon_start=1
                     /transl_table=11
                     /product="FnlB"
                     /protein_id="AAY28261.1"
                     /translation="MSWIHNMKILVTGAAGFIGRNLVFRLKEAGYNELITIVRNSSLA
                     DLEKGLKQADFIFHLAGVNRPVKESEFEEGNSNLTQQIVDILKKNNKDTPIMLSSSIQ
                     AECDNAYGKSKAAAEKIIQQYGETTNAKYYIYRLPNVFGKWCRPNYNSFIATFCHRIA
                     NDEAITINDPSAVVNLVYIDDFCSDILKLLEGANETGYRTFGPVYSVTVGEVAQLIYR
                     FKESRQTLITEDVGNGFTRALYSTWLSYLSPEQFAYTVPSYSDDRGVFCEVLKTKNAG
                     QFSFFTAHPGITRGGHYHHSKNEKFIVIRGSACFKFENIVTGERYELNVSSDDFKIVE
                     TVPGWTHDITNNGSDELVVMLWANEIFNRSEPDTIARVLS"
     gene            10331..11443
                     /gene="fnlC"
     CDS             10331..11443
                     /gene="fnlC"
                     /note="L-fucosamine synthetase"
                     /codon_start=1
                     /transl_table=11
                     /product="FnlC"
                     /protein_id="AAY28262.1"
                     /translation="MSVVGTRPEIIRLSRVLAKLDEYCDHLIVHTGQNYDYELNEVFF
                     KDLGVRKPDYFLNAAGKNAAETIGQVIIKVDEVLEQAKPEAMLVLGDTNSCISAIPAK
                     RRRIPIFHMEAGNRCFDQRVPEETNRKIVDHTADINMTYSDIAREYLLAEGVPADRII
                     KTGSPMFEVLTHYMPQIDGSDVLSRLNLTPGNFFVVSAHREENVDTPKQLAKLANILN
                     TVAKKYDVPVVVSTHPRTRNRINENGIQFHKNILLLKPLGFHDYNHLQKNARAVLSDS
                     GTITEESSIMNFPALNIREAHERPEGFEEGAVMMVGLESERVLQALEIIATQPRGKVR
                     LLRQVSDYSMPNVSDKVVRIIHSYTDYVKRVVWKQY"
     gene            11443..12651
                     /gene="wbuB"
     CDS             11443..12651
                     /gene="wbuB"
                     /note="L-fucosamine transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuB"
                     /protein_id="AAY28263.1"
                     /translation="MKLALIIDDYLPHSTRVGAKMFHELGLELLSRGHDVTVITPDIT
                     LQAIYSVSMIDGIKVWRFKSGPLKDVGKAKRAINETLLSFRAWRAFKHLIQHDTFDGI
                     VYYSPSIFWGDLVKKIKQRCQCPSYLVLRDMFPQWVIDAGMLKAGSPIEKYFRYFEKK
                     SYQQADRIGLMSDKNLEIFRQTNKGYPCEVLRNWASMIPVSASDDYHSLRQKYDLKDK
                     VIFFYGGNIGHAQDMANLMRLARNMMRYHDAHFLFIGQGDEVDLIKSLAAEWSLTNFT
                     HLPSVNQEEFKLILSEVDVGLFSLSSRHSSHNFPGKLLGYMVQSIPILGSVNGGNDLM
                     DIINKHRAGFIHVNGEDDKLFESAQLLLSDSVLRKQLGQNANVLLKSQFSIESAAHTI
                     EVRLEAGECV"
     gene            12642..13049
                     /gene="wbuC"
     CDS             12642..13049
                     /gene="wbuC"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC"
                     /protein_id="AAY28264.1"
                     /translation="MRLVDDNILDELFRTAANSERLRAHYLLHASHQEKVQRLLIAFV
                     RDSYVEPHWHELPHQWEMFVVMQGQLEVCLYEQNGEIQKKFVVGDGTGISVVEFSPGD
                     IHSVKCLSPKALMLEIKEGPFDPLKAKAFSKWL"
ORIGIN      
        1 attggtagct gtaagccaag ggcggtagcg tgtgttaata cctctattaa tcaaactgag
       61 agccgcttat ttcacagcat gctctgaagt aatacggaat aaattaagtg aaaatacttg
      121 ttactggtgg cgcaggattt attggttctg ctgtagttcg tcacattata aataatacgc
      181 aggatagtgt tgttaatgtc gataaattaa cgtacgccgg aaacctggaa tcacttgctg
      241 atgtttctga ttctgaacgc tatgtttttg aacatgcgga tatttgcgat gctactgcaa
      301 tggcgcggat ttttgctctg catcagccgg atgcagtgat gcacctggct gctgaaagcc
      361 atgtggatcg ttcaattaca ggccctgcgg catttattga aaccaatatt gttggtactt
      421 atgtcctttt ggaagcggct cgcaattact ggtctgctct tgatggcgac aagaaaaata
      481 gcttccgttt tcatcatatt tctactgacg aagtttatgg cgatctgcct catcctgacg
      541 aagtaaataa taaagaagaa ttacccttat ttactgagac aacagcttac gcaccaagca
      601 gcccttattc tgcatcaaaa gcgtccagcg atcatttagt ccgcgcgtgg aaacgtacct
      661 atggtttacc gaccattgtg actaattgct ctaacaatta tggtccttat cattttccgg
      721 aaaaattgat tccattggtt attctgaatg ctctggaagg taaagcatta cctatttacg
      781 gcaaagggga tcaaattcgc gactggttgt atgttgaaga tcatgcgcgt gcgttatata
      841 ccgtcgtaac cgaaggtaaa gcgggtgaaa cttataacat tggtggacac aacgaaaaga
      901 aaaacataga tgtagtgctc actatttgtg atttgctgga tgagattgta ccgaaagaga
      961 aatcttatcg tgagcaaatc acttatgttg ccgatcgtcc gggacacgat cgccgttatg
     1021 ccattgatgc tgagaagatt ggtcgcgaat tgggatggaa accacaggaa acgtttgaga
     1081 gcgggattcg gaagacagtg gaatggtacc tgtccaatac aaaatgggtc gaaaatgtga
     1141 aaagtggtgc ctatcagtca tggattgcac agaactatga gggccgccag taatgaatat
     1201 cctccttttt ggcaaaacag ggcatgtagg ttgggaacta cagcgtgctc tggcaccttt
     1261 gggtaatttg attgctcttg atgttcactc tactgattat tgcggtgatt ttagtaatcc
     1321 tgacggtgta gctgaaacca taagaagcat tcggcctgat attattgtca acgcagccgc
     1381 tcacaccgca gtagacaaag cagaatcaga accggagttt gcacaattac ttaacgcaac
     1441 aagtgtcgaa gcgattgcga aagcagcaaa tgaagttgga gcttgggtta tccattactc
     1501 gactgattac gtcttctctg gaaatggcga tatgccatgg ctggagacgg atacaaccgc
     1561 accactaaat gtttacggtg aaaccaagtt agccggagaa aaagcgttac aggaatattg
     1621 cgcaaagcat cttattttcc ggaccagctg ggtctatgca ggaaaaggaa ataacttcgc
     1681 caaaacgatg ttacgtctgg caaaagagcg tgaagaatta gcggttatta acgatcagtt
     1741 tggtgcgcca acaggtgctg aactgctggc tgattgtaca gcacatgcca ttcgtgtcgc
     1801 actgaataaa ccggatgtcg caggcttgta ccatttggta gccagtggta ccacaacctg
     1861 gtacgattat gctgcgctgg tttttgaaga ggcgcgcaaa gcaggcattc ccctttcact
     1921 caacaagctc aacgcagtac caacaactgc ctaccctaca ccagctcgtc gtccacataa
     1981 ctctcgcctt aatacagaaa aatttcagca gaactttgcg cttgtcttgc ctgactggca
     2041 ggttggtgtg aaacgaatgc tcaacgaatt atttacgact acagcaattt aatagttttt
     2101 gcatcttgtt cgtgatggtg gagcaagatg aattaaaagg aatgatgaaa tgaaaacgcg
     2161 taagggtatt attttagcgg gtggttctgg tactcgtctt tatcctgtga ctatggcagt
     2221 cagtaaacag ctgctaccga tttatgataa accgatgatc tattatccgc tctctacact
     2281 gatgttggcg ggtattcgcg atattttgat tatcagtacg ccacaggata ctcctcgttt
     2341 tcaacaactg ctgggtgacg gtagccagtg ggggctgaat cttcagtaca aagtgcaagc
     2401 gagtccggat ggtcttgcgc aggcatttat tatcggtgaa gagtttattg gtggtgatga
     2461 ttgtgctttg attcttggtg ataatatctt ttacggtcac gatctgccga agttaatgga
     2521 tgccgctgtt aacaaagaaa gtggtgcaac ggtatttgcc tatcacgtaa atgatcctga
     2581 acgctacggt gtcgttgagt ttgataaaaa cggtacggca ataagcctgg aagaaaaacc
     2641 gctacaacca aaaagtaatt atgcggtaac cggactttat ttctatgaca atgatgttgt
     2701 agaaatggcg aaaaacctta agccttctgc ccgtggcgaa ctggaaatta ccgatattaa
     2761 ccgtatttat atggagcagg gacgtttgtc tgtcgctatg atggggcgtg gttatgcctg
     2821 gttggatact ggtacacatc aaagtcttat tgaagcaagt aacttcattg ccaccattga
     2881 agagcgtcag ggattaaagg tatcttgccc ggaagagatt gcttaccgta aagggtttat
     2941 tggagaaaaa gaactttatg cacttgcgca gccattgcta aaaaacaatt atggaaaata
     3001 tctcactgga atattaaaaa ataatacata tgttaactaa atacacatta attaggaata
     3061 gtatattaaa tctttcaggt tatataatac caacgttggt tgcaatacca gcattaggtt
     3121 atatggcaag agagctaggt cctgaattat ttggggtata cacgctcgct atggcattag
     3181 tgggttatgc tagtgtgttt gattttggat taaccagagc aataatcaga gaaatagcta
     3241 tttatagaga tgatattgta gaaaaaagaa aaataatatc tacttcaact gtttttttga
     3301 ccgtaattgg atttgttgtt acagagctca tttttattaa tgttaataca attgtcactt
     3361 ttattaatgt atcaaaaaat aatttcgaag atgcgaatat tgcattgaaa atactgtctt
     3421 tatctatacc actactttta ttaaatcaat tgtgggtttc ggtgtttgaa ggggaagaaa
     3481 aatttgggtt gatcaatatt cagaaaacca tatcgaacac ttgcattgtt gcgttgcctg
     3541 ctgtaagtat attgatagaa agttccttga cctatgcagt atcagggctt gtagtcgggc
     3601 gagtaatatc cttaattttg tcttttttgt ttttacgtaa agagatagtt gcatcaggta
     3661 ttagatttca caccattgtc atgaaaagat tgataatgtt tggtagttgg atgacgttta
     3721 gcaatattat aagcccaatg atggtgtatt ttgatcgatt tataatttca aatatattag
     3781 gtgcaaagaa tgttggattc tatactgcac cagcagaaat aatatcaagg atgagtatta
     3841 ttccaacatc agtgtctaga gctctatttc cccgacttag taatatgagt gattttaaaa
     3901 aatttaaatg cgagctttta ttttcgtatg cgataatgat atcgatttgc gtgcctattg
     3961 ttttatttct tcttctattt tcgggtggga taatgtatta ttggttgggg ccgcagtatt
     4021 atttaaaatc ctcaagtgtt ttttctgtat tattgattgg ttttttcttt aattctcttg
     4081 cgcaactgcc tttttctgca attcaatccc taggtaattc taaaataact gcattactcc
     4141 atggttttga aattattcca tatcttataa tcttgtatat tctaactagc catttcggga
     4201 tagtaggaac ggcatatgca tggacattga gagttatgtt cgattttatt gcactatact
     4261 ttctttcatc ctggttaata aaaaagaaac actaaaaagg taattttatg aaaatagcaa
     4321 atacaaaact tgatggttta ctggttattg aacctactgt ttatgaagat gagagaggat
     4381 ttttttatga aagttataat gaaaaaaaat ttatggagac aataggagcg tcaataaaat
     4441 ttgtccaaga taaccattca aaatcgtcta aaggggtcct tcgcggcctg cattttcaaa
     4501 aaaatcctta tgaacaagcg aaactagttc gatgtatccg aggagaagtt tatgacgtag
     4561 ctgttgatat aagaaaggac tctccaacat ttggacaatg gtttggcgtg aatttatctg
     4621 atgaaaataa aaaacaatta tggataccag aaggatttgc tcatgggttt ataacattaa
     4681 gtgatgttgc agaattcgta tataagacga caaactatta tgctcctgct agtgaatatt
     4741 gtctaaaatg ggatgataaa gatttaaata ttcaatggcc tttaaaaaat gctttaatag
     4801 tatcaagtaa agatcagaaa gggaaagctt tcgctgattt cataattgaa agcaatgaag
     4861 aacaaaggtg gtaatgtgag cttaattcag agagtatctt ctcttttgat agcgttaacc
     4921 atttatctat ttttatttga tgttatttta ttagggtcag gagcatggag cattaatgta
     4981 acaggtatat caataagaaa agttgaatat ttaacaatat tattaatggt catatgcact
     5041 attaacaata taaaaggata tatttatttc tgctgtgcta ctactttctg ccttattttt
     5101 ggcgttgttg ttcctttttt aaatgatgtt aagttagaat acgcaataac agaacttttg
     5161 tctttttatg gtattttgct atgccctcta attgcgcaaa atagatatat ttcggttaac
     5221 tggaaaaaaa taaggaagtt catactattc atttgcatca taagtgcatt tattcatata
     5281 ttaatatggc tcttaggatt gatggggtct ggttatgttg atcttatcaa gcaattatgt
     5341 ataagggtat tgactgcaaa taatgatgac ttaattgata atattattat tgccgataca
     5401 ccggatggat tatttagagt gttgctgcct aatagttcct tattgattat cggattttat
     5461 ctatcattcc aacagtacct caatagaaaa aattacatac acttattaat tgtatttgta
     5521 atgctattgg ctttatatac tacatggacg agggctttat atttatctcc gattataatt
     5581 attggaggtg tactttttta taagatattt ccatttactt tgcaattggg taaaattggc
     5641 gcatataact tgctatcatt tataattctc ttttttattg tgatatctac tgttctagtc
     5701 caaccaactt tattatcatt gctgggcata gcaagtgaga gtagtgatgg aataagatat
     5761 acacaggtta tttggatatt taatacgttt atgaatagtc ctgttctggg tactggcttg
     5821 ggggggagtg cagaagacgt acgttcatta attgctccat ggacttacga gatgagttta
     5881 ttggcgctga ttatgaaatt aggtctagtt ggtgttttcc ttttcatttc tattagtgct
     5941 ataaaaatac ctgaattttt ggagggtttt tttgatggga gaaggatacc agttaaaaaa
     6001 gtcgtatgga tttatttttc tttatctgtg ctaatgatgt tttcaaccaa tccatttatg
     6061 ctttcattcc caggggtaac aattactttg tttattttat gtgagttgaa tagctttaaa
     6121 atcgagaaag gtaatatatg atacaacgag ctgtcatcat tgtttgttat catccggtaa
     6181 aaaataagct tgaaaacttg gttgacaagg tttccggccc aggaacaatt gtttatatcg
     6241 ctaataatgg tggtataacc gccgagttaa actacacact gaatcaaaaa aaagcaatag
     6301 ttattaactt tgatagaaac ttgggtttgg gcgaggcgat taatagtatt gctgaaatca
     6361 ttccagaatc tgttgaggct atatttacat ttgaccaaga tacatcacct cctgatgatt
     6421 atataataaa aacatggaca cattttcgta aattgatgtc agaggggata aatttaggcg
     6481 tgttaacgcc taaattcata gactctcggt ctggatattt atatcagcaa aaacctaaag
     6541 ggtattataa tagatataca gaattgctag ttactttaca atcaggtatg tgtataccta
     6601 aaaatgtttg gaaagaggaa aagtttaata gcgatttatt tatagagttt gttgatacag
     6661 aatggtgcta tagaattcac agtaaaaaat ataaagttat ccaaatggat gatattataa
     6721 tgagccatga ggtttctgaa ttatcgccta aaaaaatact gtcattctca ttgatgaaat
     6781 acaaacctat aagacgatat tatttcttta gaaatgcaat gtttttgcta caacaaaatt
     6841 atgttccact atatagcaaa ataagattgc ttacaggtat gtgcaatcga attttatcag
     6901 ttgctttaat ggatgagagt aaattggact catatataca ttgtatacga ggaataaaag
     6961 acggagtaaa acttgggaaa aatgagaaaa atattaatga tatgcactaa gtatccacta
     7021 gacctcaata gtacgtggtt aactagagaa cttgctgaag aatttaataa cagggggtac
     7081 gtgattgatg taatctgtat tgattggggt gttttacaaa agagaaaaaa aataagctta
     7141 aataacatca atatatataa tgtccctgct ttaggttgta aaaaaacgag ctttttaaat
     7201 tttttcataa aatggggtgg atcttcatta tatgccgctc tactaaattt cagagttttt
     7261 tttaaaagac atgacctagt gatcagtttc tcaccatgcg catcgtcttg gtttgccata
     7321 atactaggta tatttttttc taagaaatca ttactcatct attgggattt ttttccaatt
     7381 catcaagtac agattgattt aattaaagga agatttaaaa ctaagattct aaaatgtttt
     7441 gaaaaagcat tagtcaaaat gtttgattat ataggttgta tgtcaaaagg gaattgtgaa
     7501 ttttttgttg aatacttcaa aactcgacga gaaaaggttt ttgagctgcc aatttggagt
     7561 gaaggattaa gaataaatgg cttaataaaa agtaaaagtg ttagttttat tgattcaaat
     7621 tcaatatatt tcgtttttgg agggcaaatt gattacgggc gtaggataga atgcatatta
     7681 tcagctgcta aaattgctca taaaaaaaat aataaaatta aaaccatcat catagggcga
     7741 ggaagacttg ttgatcaggt aattaaagat gctgagagta tagaaaatgg tattatttac
     7801 tcagacttta ttccacgaaa tgattatctt acactggttt ctgtttgtcg cgcgggtctt
     7861 attgctactg tacctaatgt ttcagtccca acgtacccat caaaatgctt ggattatatg
     7921 aagctcagta tcccaatcat tgcttcaatt gaggacacaa cagatttcgg tgaaataata
     7981 ataaatgcag gtgcggggtt gtgttgtaat gcgggagatt ctaatgccct cgccgccgca
     8041 atgctatcat tggctgaaga cgaattatta gctagtcaaa tgggagaaaa agcaaattta
     8101 ttttttaaaa atagacatga agttaaaacc gtagtaaaca atttgttgga acatattaaa
     8161 gaggattgat aagatgttta aagataaaac acttttaatt acaggcggta ctggttcgtt
     8221 tggaaatgct gttttacgtc gatttttaga cactgacatt gctgagatcc gtattttcag
     8281 tcgtgatgaa aaaaaacaag atgatatgcg gaaaaaatat aatagtgata aattgaggtt
     8341 ttatattgga gatgttaggg actttagcag tatacggaat gcaagtagag gtgttgattt
     8401 catttatcat gcagcagctc ttaagcaagt accatcatgt gagtttcacc caatggaggc
     8461 tgttaaaact aatgttttag gtactgaaaa tgttctcgaa gctgctatat caaatcatgt
     8521 gcgtaaagtt gtttgcttaa gtacggacaa agctgtttat cctatcaatg ctatgggtat
     8581 ttcaaaagcc atgatggaaa aagttatggt agctaaatcg cgtaatgttg atagctccaa
     8641 aacagtcata tgcggaactc gttatggcaa cgttatggct tcacgtggat cggtcattcc
     8701 attatttgtt gatctaatca aatctggtaa accattgacc attaccgatc ccaatatgac
     8761 tcgtttcatg atgacgcttg aggatgctgt tgatctggtc ctttatgcct tcgagcatgg
     8821 aaataacggt gacattttcg ttcagaaagc acctgcggca acaattcaaa cattagccat
     8881 tgcacttaag gaattgctaa atgtccatga acatccagtc aatgttattg gaactcgaca
     8941 cggggaaaaa ctttacgaag cattattgag ccgagaggaa atgattgcag cggaagatat
     9001 gggtgattat tatcgtgttc caccagatct ccgcgatttg aactatggaa aatatgtgga
     9061 acatggtgac cgtcgtatct cggaagtgga agattataac tctcataata ctgagagatt
     9121 agatgttgag ggaatgaaaa aattactgct aaaacttcct tttatccggg cacttcgttc
     9181 cggtgaagat tatgagttgg attcataata tgaaaatttt agttactggc gcggcagggt
     9241 ttatcggtcg aaatttggtt ttccgcctta aggaagctgg atataacgaa cttattacga
     9301 tagttcgtaa ctcttctttg gcggatttag aaaagggact taagcaggca gatttcattt
     9361 ttcaccttgc tggagtaaat cgtcctgtga aggagagtga atttgaagag gggaatagca
     9421 atctaactca acagattgtt gatattttga aaaaaaataa taaagatact cctatcatgc
     9481 ttagttcttc catccaggct gaatgtgata acgcttatgg aaagagtaaa gcagctgcgg
     9541 aaaaaatcat tcagcagtac ggggaaacga caaatgctaa atattatatt tatcgcttgc
     9601 cgaatgtatt cggtaagtgg tgtcgaccaa attataactc ctttatagca actttctgcc
     9661 atcgcattgc aaatgatgaa gctattacaa ttaatgatcc ttcagcagtt gtaaatctgg
     9721 tgtatataga tgatttttgt tctgacattt taaagctatt agaaggagcg aacgaaactg
     9781 gttacaggac atttggtcca gtttattctg ttactgttgg tgaagtggca caattaattt
     9841 atcgatttaa agaaagccgc caaacattaa tcaccgaaga tgtaggtaat ggatttacac
     9901 gtgcattgta ctcaacatgg ttaagttacc tttctcctga acagtttgcg tatacggttc
     9961 cttcttatag tgatgacaga ggggtattct gtgaagtatt gaaaacgaaa aacgcgggtc
    10021 agttttcttt ttttactgcg catccaggaa ttactcgggg tggtcattat catcattcca
    10081 aaaatgagaa atttattgtc atccgaggaa gtgcttgttt caaatttgaa aatattgtca
    10141 cgggtgaacg atatgaactt aatgtttcat cagatgattt taaaattgtt gaaacagttc
    10201 cgggatggac gcatgacatt actaataatg gctcggatga gctagttgtt atgctttggg
    10261 caaatgaaat atttaatcgt tctgaaccag atactatagc gagagtttta tcgtgaaaaa
    10321 attgaaagtc atgtcggttg ttgggactcg tccagaaatt attcgactct cgcgtgtcct
    10381 tgcaaaatta gatgaatatt gtgatcacct tattgttcat actggtcaaa actacgatta
    10441 tgaattgaat gaggtttttt tcaaagattt gggtgttcgc aaacctgatt attttcttaa
    10501 tgccgccggt aaaaatgcag cagagacaat tggacaagtt atcattaaag ttgatgaggt
    10561 tcttgaacag gcaaaaccag aagccatgtt agttcttggt gatactaact cctgtatttc
    10621 agcaatacca gcaaagcgtc gaagaattcc gatcttccat atggaggcgg ggaatcgttg
    10681 ttttgaccaa cgcgtaccgg aagaaactaa cagaaaaata gttgaccata ccgctgatat
    10741 caatatgaca tatagtgata tcgcgcgtga atatcttctg gctgaaggtg taccagccga
    10801 tagaattatt aaaaccggta gcccaatgtt tgaagtactc actcattata tgccgcagat
    10861 tgatggttcc gatgtacttt ctcgcctgaa tttaacacct gggaatttct ttgtggtaag
    10921 tgcccacaga gaagaaaatg ttgatactcc taaacagctt gcgaaactgg cgaatatact
    10981 taataccgta gctaaaaaat atgatgtccc ggtagtcgtt tctactcatc ctcgcactcg
    11041 taaccgcatc aacgaaaacg gtattcaatt ccataaaaat atcttgcttc ttaagccatt
    11101 aggatttcac gattataacc atctgcaaaa aaatgcacgt gctgttttat cggatagtgg
    11161 gactattaca gaagagtcct ccattatgaa cttccctgca ctcaatatac gagaagcgca
    11221 cgaacgcccg gaaggcttcg aagaaggggc agtaatgatg gtcggtcttg aatctgagcg
    11281 cgtcttacag gcattagaaa ttattgcaac acagcctcgt ggaaaagtac gcttacttcg
    11341 tcaggtcagt gactatagca tgccaaatgt ttcagataaa gttgtgcgta ttatccattc
    11401 atacactgac tacgttaaac gggttgtctg gaagcaatac taatgaaact tgcattaatc
    11461 attgatgatt atttgcccca cagcacacgt gttggggcta aaatgtttca tgagttaggc
    11521 cttgaattgc tgagcagagg ccatgatgta actgtaatta cgcctgacat cacattacaa
    11581 gcaatctatt ctgttagtat gattgatggt ataaaggttt ggcgtttcaa aagtggacct
    11641 ttaaaggatg taggtaaggc taaacgagcc ataaatgaaa ctcttttatc ttttcgtgca
    11701 tggcgcgcat ttaagcacct cattcagcat gatacatttg atggtatcgt ttattattcc
    11761 ccctctattt tttggggaga cttggttaaa aaaataaaac agcgatgcca gtgcccaagc
    11821 tatctggtcc taagggatat gtttccacag tgggttattg atgcaggtat gttgaaagcc
    11881 ggttcgccaa ttgaaaaata tttcaggtat tttgaaaaaa aatcatatca gcaggctgac
    11941 cggatagggt taatgtccga taagaatctt gagatatttc gtcagaccaa taaaggttat
    12001 ccgtgtgaag ttttacgtaa ttgggcctca atgattcctg tgtctgccag cgatgattat
    12061 cattcacttc gtcaaaaata cgatctaaaa gataaagtta tttttttcta tggcggtaat
    12121 attgggcatg ctcaagatat ggcaaactta atgcgccttg cgcgtaatat gatgcgttat
    12181 catgatgctc atttcctgtt tattgggcag ggtgatgaag ttgacctgat aaaatctctt
    12241 gctgcagaat ggagtttaac taatttcact catctacctt cagtgaacca ggaagagttt
    12301 aaattaattt tatctgaagt tgatgtcggc ctattctccc tttcatctcg ccattcttca
    12361 cataatttcc ccgggaaatt actagggtat atggttcaat caatcccgat ccttgggagt
    12421 gtgaatggcg gcaatgattt aatggatata attaataagc acagggccgg ttttattcat
    12481 gttaatggtg aagatgataa actgtttgaa tctgcacaat tgcttcttag tgattcagtt
    12541 ttaagaaaac agttaggtca gaacgctaat gtgttgttaa agtctcaatt ttcgattgaa
    12601 tcggcggcac atactatcga agtccgactg gaggcaggag aatgcgttta gttgatgaca
    12661 atattctgga tgaacttttt cgcactgcag caaattctga acgtttgcgc gctcattatt
    12721 tattgcacgc atctcatcag gagaaggttc aacgtttact tattgcattt gtacgcgaca
    12781 gttatgttga accccattgg catgagttac cgcatcagtg ggaaatgttt gtcgtcatgc
    12841 aagggcaatt agaagtttgt ttgtatgagc aaaatggtga gatccaaaaa aaatttgttg
    12901 ttggagacgg tacgggaata agcgtcgtgg aattttcccc aggagatata catagtgtca
    12961 aatgcctgtc accaaaagcc cttatgttag agataaagga ggggccattt gacccactga
    13021 aagctaaggc tttttctaag tggttatagg gcgatacacc accgtttatt cttctatttt
    13081 attctataca tgctgggtta ccatcttagc ttcttcaagc cgcgcaaccc cgcggtgatc
    13141 acccctgaca ggagtaaaca atgccaagca acagatcggc gtcgtcggta tggcagtg
//

</pre>