<pre>LOCUS       KJ778798                9694 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain E29518-83 serotype O176:H- O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ778798
VERSION     KJ778798.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9694)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9694)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Swimley,M.,
            Baranzoni,G.M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E29518-83"
                     /serotype="O176:H-"
                     /db_xref="taxon:562"
     misc_feature    1..9694
                     /note="O-antigen gene cluster"
     CDS             197..781
                     /codon_start=1
                     /transl_table=11
                     /product="DDE_Tnp_1-associated family protein"
                     /protein_id="AIG62737.1"
                     /translation="MELKKLMEHFSIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDREDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSND
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSVVLGRLRRMRNPMRLQPFLNFLT
                     CWILKEKSSQLMRWVARKILQRRYKNREVIIYSR"
     CDS             complement(1293..1538)
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="AIG62738.1"
                     /translation="MAKTVSTSKKNHKQHSPEFRSEDLKLAERIGIAAAARELSLYES
                     QLYNWRSKQQNQQTSSERELETGQDYESPLHARTDAR"
     CDS             1667..2842
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferases group 1"
                     /protein_id="AIG62739.1"
                     /translation="MKILYVSARFPWPLLTGDALRAYNQIKSLSKFHSIDVFSIEAPP
                     VSRGDIDLYISESMSSQISKLQKIINIISYGKDTAFQCAMYNDMKSWQELRRLIISNK
                     YDVIIFQLVRLEYLIKKTVELKNQLGLDISIYCDYVDALSLNMENRTATEGLLLKKIC
                     SSESRKLKKIENEIYDLVDVKIIISERDGKYINNKGFNVVPNGVTIPVRNESLYHNES
                     DGVVNLSFWGNMSYYPNVRAAQYLADIFRQLEVGKYKLHIIGAKPSKKVRDLGNSDNI
                     IVHGFIDDLPNFLANMDLAVFPIFDGSGLQNKVLEAFALEIPVITTNVVLDSIPKLKK
                     FAMVANCKADFIRNIESFELSKEKSPSESGVLKVLNEHYNWENINQVMIQNEKNTAF"
     CDS             2820..3839
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferases group 1"
                     /protein_id="AIG62740.1"
                     /translation="MKKILPFNFYNGVLYRGIPIYSSNLKIGLEKEGCKVNEWKCPNA
                     LNKMPRFLLDLLFVFSEQILMPLICFFGRYDKVIYPYNSCSILSAIVGKSLLIIHDFI
                     PNKKNKSKLSLSALYIVLTQRVHTLLKRDVAFVSRTTQRIGKNVSWLKKCGVYLFPNS
                     FYILERELANLKCKFFQKDEYAVLISGNGTNKEFTKAMELWKGCNICYSHHLKVIGLG
                     GHQEWAQKILKSLNINNVSILPVLSDSALLCEIINASFVWAHSNHEGFGRPVIEGRMC
                     GKNVLATNISAFREHKDDNVFLYNQNSFCENLRLAHSFGCKPNYKIKYHLILEEQIKK
                     WLMKN"
     gene            3821..5257
                     /gene="manC"
     CDS             3821..5257
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase 1"
                     /protein_id="AIG62741.1"
                     /translation="MVDEKLIPIIMAGGTGSRLWPLSRELYPKQFLSLQHELTMLQST
                     IMRLDGITNCEPVVICNESHRFIVAEQLLQIQKLAKNIILEPVGRNTAPAVALAALTA
                     KAIHSDEDPLLLILAADHVFTDEIAFRNSISKAIPFANVGKLVTFGILPNKPETGYGY
                     ILRGKKIKLESDDYAFEVERFVEKPDIETAEEYVSNGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILLACEKSISEMSFDLDFIRINEEAFASCPDESIDYAVMEHTSDAVVIPMDAG
                     WSDVGSWSSLWEVSIQNSEGNVCHGDVINYKTKNSYVYSESSLVTTIGVKDLVIVQTK
                     DALLVAHRDAVQDVKKVVEKLKSDGRPEYQEHREVYRPWGKYDSIDSGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTINGDTRLIGENESIFIPLGARHCLENPGKI
                     PLDLIEVRSGPYLEEDDIVRLSDRYGRK"
     gene            5308..6678
                     /gene="algC"
     CDS             5308..6678
                     /gene="algC"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="AIG62742.1"
                     /translation="MNILSCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINCTVHNQKSIINK
                     ILNEFQAQAKNIDYTDGISMEFERWRFNLRSSNTEPVLRLNVESKKDKDLMEEKTKEL
                     LSRIVK"
     gene            6801..8132
                     /gene="wzy"
     CDS             6801..8132
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AIG62743.1"
                     /translation="MILYKERSVQANVITKLMAICIVICFAMFILLLKNMLGNNGYAT
                     LLHVLAPFLSICLIAVMIFFYIKRASFISLQLMYAFAFSLIIGVPGIYLLNFRNPVKG
                     FEIVCLWAILLNIVLYFCSYNNKTITQRKKVNKYFVVIFVIVGICQLIKTGLYFIFIV
                     NSGVGHLAIYTEGDALVSQVPFVIRAISGMSLIMSLATFYYRASTGVRWFSFLLLASE
                     LLIGIRSKFFFSLICIIILSLYCNKELIKKRFLKISRLLYLVIGFVLFSLVSYYREGY
                     DINFVKYMVIVLDSLSSTLAGLQDIFVLPDSQGWNRLDPHLIITQLFPISGLGFLNST
                     QIYKEFSVIVLGDISSGIALSSSGILESTILDVDFNFFIYLSYLLLMLSLIQHFLNSP
                     KVFLNFIAIAMLPGFLYSIRGELILPIAYIIKSSPIIIVSNFLVSDKNADE"
     gene            8135..9367
                     /gene="wzx"
     CDS             8135..9367
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AIG62744.1"
                     /translation="MIRDIFNDGIYSLINKIGYFGAKTIAIILITRTLGVDEGSQFIF
                     LISIIEIMRVIGDFGVDVYVIRKYGDVPNKERLLCTVFYQKIIVGFVFAAILIVYCSI
                     LKYNASIYVPLSLSIIFSLLFNLSSSYFQSLNINKAITTSIISASIFIGVLFSSFYYF
                     NVVVNAYHFLVIEIVYCMSVSFTLINKTDLKIIRQFKKVDISAILYLYRNTYSIGITA
                     LIVIIYSRLDNIYLKLYDEQSLAVYGQIFRFVDPLVMISSVFSTVAYAKFSNFKLHNN
                     EASIKSFLLMIVIYILVSFLIYYYVLISIGQWFILPTSLFKPLLIGFLCMASIKCMNG
                     SLTAILQSQGLYKIGLYISILCIIIAVPLMYVFVKHYHALGCIYAVLIVETVSFIFLS
                     ASVYFLTSNRNHKDLNVK"
ORIGIN      
        1 tagacatgca tgcagtgctc tggtagctgt aaagccaggg gcggtagcgt gccaaggaaa
       61 gatcacgtaa cgctgctttt ttggactaaa taattcgcat tttatgttta aaattgagct
      121 attcctcatt acctgaagct gtttatttat tgcttataca tgatcaaata ctccttacat
      181 aattaaggag aacaaaatgg aacttaaaaa attgatggaa catttttcta ttattcccga
      241 ttacagacaa gcctggaaag tggaacataa attgtcagat attctactgt tgactatttg
      301 tgccgtcatt tctggtgcag aaggctggga agatagagag gattttggag aaacacatct
      361 cgattttttg aagcaatatg gtgattttga aaatggtatt cctgttcacg ataccattgc
      421 cagagttgta tcctgtatca gtcctgcaaa atttcacgag tgctttatta actggatgcg
      481 tgactgccat tcctcaaatg ataaagacgt tattgcaatt gatggaaaaa cgctccggca
      541 ctcttatgac aagagtcgcc gcaagggagc gattcatgtc attagtgcgt tctcaacaat
      601 gcacagtgtg gttctcggca gattaagacg gatgagaaat ccaatgagat tacagccatt
      661 cctgaacttc ttaacgtgct ggatattaaa ggaaaaatca tcacaactga tgcgatgggt
      721 tgccagaaag atattgcaga gaagatacaa aaacagggag gtgattattt attcgcggta
      781 aaaggaaacc aggggcggct aaataaagcc tttgaggaaa aatttccgct gaaagaatta
      841 aataatccag agcatgacag ttacgcaatc agtgaaaaga gtcacggcag agaagaaatc
      901 cgtcttcata ttgtttgcga tgtccctgat gaacttattg atttcacgtt tgaatggaaa
      961 tggctgaaga aattatgcgt ggcagtctcc tttcggtcca taatagcaga acaaaagaaa
     1021 gagccagaaa tgacggtcag atattatatc agttctgatg atttaacccc agagaagttc
     1081 gccagagcaa tccgaaacca ctggcacgtg gagaataagc tgcactggcg tctggatgtg
     1141 gtaatgaatg aagacggctg caaaataaga agaggaaacg cagcagaatt attttcaggg
     1201 atacggcaca tcgctatcaa tattttgacg aatgataagg tattcaaggc agggttaaga
     1261 cgtaagatgc gaaaagcagc catggacaga aactatctgg cgtcagtcct tgcgtggagc
     1321 gggctttcgt aatcttgccc tgtctccagc tcacgttcag aggaagtctg ctgattttgc
     1381 tgtttactgc gccagttgta gagctgcgat tcatacaggc tgagttcgcg ggctgcggca
     1441 gcaataccga tgcgttccgc aagcttcaga tcttcactgc gaaattcagg cgaatgctgt
     1501 ttatggtttt ttttactggt tgatactgtt tttgccatgt gagagtctcc tctgactgag
     1561 agtatactca cttagctgcg tgtccactat tgctgggtaa gatcaatact gaaatattaa
     1621 tttgaagctt taacgatcat ttttatgtag aatttcaagg ttagttatga aaatattata
     1681 tgtaagtgca cgctttccat ggccattact gactggcgat gctctccgag catacaacca
     1741 aattaaatca ctttcaaagt tccactcgat agatgtattt tctattgaag ctcctcctgt
     1801 atctagaggg gatatagact tatacataag tgagagtatg tctagtcaaa tttcaaaatt
     1861 acaaaaaata ataaacatca tatcttacgg taaagataca gcatttcaat gtgcaatgta
     1921 taacgacatg aaatcgtggc aagaacttag aaggttaata atatcaaata aatatgatgt
     1981 gattatattt caattggtta ggttggaata tttaataaag aaaactgtag aattaaagaa
     2041 tcagcttggt ttagatattt cgatatattg tgattatgtt gatgcacttt cattaaatat
     2101 ggaaaatcgt acagcgaccg aaggtttact attgaaaaaa atatgttcaa gtgaaagccg
     2161 gaagcttaaa aaaattgaga atgaaatata tgacttagtt gatgttaaaa taattatatc
     2221 agaaagagat ggcaaatata taaataataa aggatttaat gttgttccta atggagtgac
     2281 aatcccagtg cgcaatgaaa gcttatatca taacgaaagt gatggggttg tgaatttgtc
     2341 attttggggt aatatgagtt attatcccaa tgttagagcg gctcaatatt tagctgatat
     2401 atttaggcaa ttagaagttg gtaaatataa actccatatt attggtgcta aaccaagcaa
     2461 aaaggtacgt gatttaggaa atagtgataa tataattgtt catggtttta tagatgactt
     2521 acctaatttt ttggcgaata tggatttggc ggtatttcct atttttgatg gttctgggtt
     2581 gcaaaataaa gtactagaag cgtttgcttt agaaattcct gtaatcacaa caaatgttgt
     2641 attagactca ataccaaaat taaaaaaatt tgctatggtt gcaaactgta aagcggattt
     2701 tatacggaat atcgaatcct ttgaattatc gaaggaaaaa tcaccttcag agtctggtgt
     2761 attaaaagtt ttaaatgagc attataattg ggaaaatatc aatcaggtaa tgatacaaaa
     2821 tgaaaaaaat actgcctttt aatttttata acggggtgtt atatagaggt atacctattt
     2881 attcatctaa cttaaagata ggtttagaga aagaaggatg taaagttaat gaatggaaat
     2941 gtccaaatgc attaaataaa atgccaaggt ttttattgga tcttttgttt gtttttagtg
     3001 aacaaatttt aatgccttta atttgttttt ttggacgtta tgataaggtt atatatcctt
     3061 ataattcatg ttctatttta tccgctatag ttggaaaaag tttgctgatt atacatgatt
     3121 ttatcccaaa taaaaaaaac aaatcaaaat taagcttatc tgcattatat attgttctga
     3181 ctcagcgagt gcatactctt ttaaaaagag atgtggcttt tgtatctaga acaacgcaaa
     3241 gaatagggaa aaacgtatct tggttaaaaa aatgtggagt atatctattc cccaattcat
     3301 tctatatttt agagcgtgaa ttggcaaatt tgaaatgtaa gttctttcaa aaggatgaat
     3361 atgctgttct tatatctggc aacggaacaa ataaagaatt tacaaaagca atggaattgt
     3421 ggaaaggatg taatatttgt tattctcatc atctaaaagt tatagggtta gggggacatc
     3481 aggaatgggc gcaaaaaata ttaaaatcgc ttaatattaa taatgtttct attttacctg
     3541 ttctttcaga cagtgcgttg ctttgcgaaa ttattaatgc ttcatttgtg tgggcgcatt
     3601 caaatcacga aggatttggt cgccctgtta ttgaaggtcg tatgtgtggg aaaaatgtat
     3661 tagcaactaa tatatccgca ttcagagagc ataaagatga taatgtcttt ttatataatc
     3721 aaaactcttt ttgcgaaaac ttaagattag cccattcatt tggttgtaaa ccgaactata
     3781 agattaagta tcatttaatt ttagaggaac agataaaaaa atggttgatg aaaaattaat
     3841 ccctataata atggctggtg gtactggcag tagattgtgg ccattgtcaa gagaactcta
     3901 cccaaaacaa tttctttcct tgcaacatga attaacaatg ctgcaatcaa ccattatgcg
     3961 actagatggt attacaaact gtgagccagt tgttatttgt aatgaaagtc atcggtttat
     4021 cgtcgctgag cagttgttac aaattcagaa gttagcaaaa aatataatat tagagcctgt
     4081 aggacgtaac acagccccag ctgttgcgtt agctgcgtta acagcaaaag caattcactc
     4141 agatgaagat ccattattac taatattagc tgcagaccat gtttttactg acgaaatagc
     4201 atttagaaac tctattagta aagcgatacc gtttgctaat gtcgggaagc ttgtaacttt
     4261 tggtattctg ccaaataagc ctgaaacagg gtatggatat attctacgtg gaaaaaaaat
     4321 caaacttgaa agtgatgatt atgctttcga agtagagcgt tttgttgaaa aaccagatat
     4381 tgaaacagct gaagaatatg tttcaaatgg cgaatattac tggaatagtg ggatgtttct
     4441 ttttcgcgca ggacgttatc ttgaagagct taaaaaatat cgtccagata tactattagc
     4501 ttgcgaaaaa tcgatatcag agatgagttt tgatcttgat tttattcgaa ttaacgagga
     4561 agcgtttgcc tcctgtccag atgagtctat tgattatgct gttatggagc atacatctga
     4621 tgctgtcgtt atacctatgg atgcagggtg gagcgatgtt ggctcttggt catctttatg
     4681 ggaggtaagt attcaaaact cggaagggaa cgtttgccac ggtgatgtga ttaattacaa
     4741 aactaaaaat agttatgtgt attctgagtc tagcttagtc accacaatcg gggtgaaaga
     4801 tttagtaatt gttcaaacta aagatgcgtt actagtagct caccgtgatg ctgtacagga
     4861 tgttaaaaaa gttgtcgaaa aactcaaatc agatggtcgt cctgagtatc aagaacatcg
     4921 tgaggtatat cgtccgtggg gtaagtatga ttcaatcgat agcggtgatc gttatcaagt
     4981 aaaacgtatt acagtaaaac ccggcgaagg tctatcagtg caaatgcacc atcaccgtgc
     5041 agagcactgg gttgtggtgg ctggtacggc taaagtaacc atcaatggtg atactagatt
     5101 aattggtgaa aatgagtcta tatttattcc attaggagca cgtcattgtc ttgaaaaccc
     5161 ggggaaaatt cctcttgatc tgattgaggt gcgttccggg ccttatcttg aggaagatga
     5221 tattgtgcgt ttatcggatc gttatgggcg caaatgaaat tactcattta tttctttaaa
     5281 taaatcaaac gttaagtaaa ttgaaaaatg aatatattat cttgctttaa agcctatgat
     5341 attcgcggga aattaggcga agaactaaat gaagatatcg cctggcgcat tggtcgcgcc
     5401 tatggcgaat ttctcaaacc gaaaaccatt gtgttaggcg gtgatgtccg cctgaccagc
     5461 gaaaccttaa aactggcgct ggcgaaaggt ttacaggatg cgggcgtcga tgtgctggat
     5521 attggcatgt ccggcaccga agagatctat ttcgccacgt tccatctcgg cgtggatggc
     5581 ggcattgaag ttaccgccag ccataacccg atggattata acggcatgaa gctggtgcgc
     5641 gaaggggctc gcccgatcag cggtgatacc ggactacgag atgtccagcg tctggcagaa
     5701 gccaacgact ttcctcccgt cgatgaaacc aaacgcggtc gctatcagca aatcaatctg
     5761 cgtgacgctt acgttgatca cctgttcggt tatatcaacg tcaaaaacct cacgccgctc
     5821 aagctggtga tcaactccgg gaacggcgca gcgggtccgg tggtggacgc cattgaagcc
     5881 cgctttaaag ccctcggcgc acctgtggaa ttaatcaaag tgcacaacac gccggacggc
     5941 aatttcccca acggtattcc taacccgcta ctgccggaat gtcgcgacga cacccgcaat
     6001 gcggtcatca aacacggtgc ggatatgggc attgcttttg atggcgattt tgaccgctgc
     6061 ttcctgtttg acgaaaaagg gcagtttatt gagggttact acattgtcgg tctgctggca
     6121 gaagcgttcc tcgaaaaaaa tcccggcgcg aagatcatcc atgatccgcg tctctcctgg
     6181 aacaccgttg atgtggtgac tgctgcaggc ggcacgccgg taatgtcgaa aaccggacac
     6241 gcctttatta aagaacgtat gcgcaaggaa gacgccatct acggtggcga aatgagcgcc
     6301 caccactatt tccgtgattt cgcttactgc gacagcggca tgatcccgtg gctgctggtc
     6361 gccgaactgg tgtgcctgaa aggaaaaacg ctgggcgaac tggtgcgcga ccggatggcg
     6421 gcgttcccgg caagcggtga gataaattgt actgttcata atcaaaaaag tattattaat
     6481 aagatactta atgaatttca agctcaagca aaaaatatag actatactga tgggataagt
     6541 atggaattcg aaaggtggcg ttttaatttg agaagctcga atacagagcc agtattacgt
     6601 ttaaatgttg aatccaagaa ggataaagat ttaatggaag aaaagactaa ggaattatta
     6661 agtagaatag taaaataata atatatagca tgtcaaatac tcttaatttg acatgctaaa
     6721 attaaaatca tgaatgtaaa atgtgactac gagagttcta attcttttca atatccacta
     6781 cccagtttag taaacaaaaa atgattctat ataaagaacg aagtgtacag gcaaatgtta
     6841 ttacaaaatt aatggcgata tgtattgtta tatgttttgc catgtttata ttgttgctta
     6901 agaatatgtt aggcaataac ggatatgcta cgctcttaca tgttctggct ccgttcttat
     6961 ctatctgttt aattgctgta atgattttct tttatataaa aagagcgtca tttatttcgc
     7021 tacaattgat gtatgccttt gcattttcat taattattgg cgtgccaggt atatatctat
     7081 tgaattttag gaaccctgta aaaggttttg aaatagtatg tttgtgggct attttgctga
     7141 atatagtgct ttatttttgc tcatacaata acaaaactat aacacaaaga aaaaaagtta
     7201 ataaatattt tgttgtaata tttgttattg tgggtatatg tcaattgatt aaaacagggt
     7261 tatatttcat atttattgtt aactctggag ttggacattt ggcaatatac accgagggag
     7321 acgcattagt atctcaagtt ccttttgtta tacgagcaat tagtggtatg tcgttgatta
     7381 tgtctttggc tactttttat tatagagctt ctacgggggt acgatggttt agttttcttt
     7441 tgttagcttc ggaactactt attggcatta gaagtaagtt ttttttctct ctaatatgta
     7501 taataatttt gtctttatat tgtaataaag agttgataaa aaaaagattt ttaaagatct
     7561 cgagactatt gtatttagta attggatttg tgttattttc acttgtctca tattatagag
     7621 aagggtatga tataaatttc gttaaatata tggttatagt actggactca ctttcctcta
     7681 ctttagctgg cttacaagat atattcgttt tacctgattc tcaaggttgg aatagattag
     7741 atcctcatct aataataacg caacttttcc caataagtgg gttgggattc ttaaacagta
     7801 cacagatata taaagagttt tccgttattg ttcttggtga tatttcaagt gggatagctc
     7861 tgtcatcctc gggtatatta gaatcaacta ttttagatgt tgattttaat tttttcattt
     7921 acttatccta tttactgtta atgctttctt taattcaaca ttttctaaac agtccaaaag
     7981 tattcttaaa ttttatcgca attgctatgc ttccaggatt tttatattca ataaggggag
     8041 aattgatttt gccaattgca tatattatta aatcttctcc tattataatt gtatcaaatt
     8101 ttttggtctc agataaaaat gcggatgagt gaacatgatt agagatattt tcaatgatgg
     8161 catatattct ctcatcaata aaataggtta ttttggtgca aagacaatag ctataatatt
     8221 aattactaga actttaggtg ttgacgaagg aagtcaattt atatttctta ttagtatcat
     8281 agaaataatg cgagtaatag gtgattttgg tgttgatgtg tatgtgatta gaaaatatgg
     8341 ggacgtgcct aataaggaaa ggcttttatg cactgttttt tatcaaaaaa ttatagtcgg
     8401 ctttgtcttt gcagcgatac taatagtgta ttgttcaatt ttaaaatata acgcgagcat
     8461 atacgttccg ttatctttgt caataatttt ttcattatta tttaatcttt ccagtagcta
     8521 tttccaatcc ttaaacatta ataaggctat taccacatca attatcagtg ccagtatttt
     8581 tattggtgta ttgttctcat ctttttatta ttttaatgtt gttgttaatg cttatcactt
     8641 tttagtgatt gaaatagttt attgcatgtc agtatctttt acgctaatta ataaaaccga
     8701 tctaaaaata attaggcaat ttaagaaggt tgatatatcg gcaattctat atttataccg
     8761 gaatacttat agcattggta ttacagcatt aattgtaatt atctatagta gattggataa
     8821 catctattta aaactttacg atgagcaaag ccttgctgtt tatggtcaaa tttttagatt
     8881 tgttgatcca ttggttatga tttcttctgt tttttcaaca gtagcatatg caaaattcag
     8941 caattttaag ttacataata acgaggcatc tataaaatca tttcttttaa tgatagtaat
     9001 ctatattctt gttagtttcc ttatttacta ttatgtcctc atctcaattg ggcaatggtt
     9061 tatattacca accagtttat ttaagccatt gcttattggt tttttgtgta tggcttcgat
     9121 taagtgtatg aatggttcac ttaccgccat attacagtct caaggtttat ataaaatagg
     9181 attgtatatt tcaatacttt gtattataat tgcagtgccg cttatgtatg tgtttgtaaa
     9241 acattatcat gcattagggt gtatttatgc agtacttatt gttgagactg ttagttttat
     9301 atttttaagt gcaagcgtat attttttgac ctctaatagg aatcataaag atttaaatgt
     9361 caaatagaaa gaaattgact gtattttcag ggtaattagt ctcttaatgt tacaatttca
     9421 cgtcactttt ttatgtttga cattttcgtt ataaacttaa tattttgcct gatccttatt
     9481 gtcttttatg gaacagccct tatacaagct gggtatttgt tatcaaaaat agctaaataa
     9541 ttattattaa catagttatt ctactaatct aaattttaac cgtgcttttt acataagccg
     9601 ccacagcggt tatcatccct gacaggagta aacaatgtca aagcaacaga tcggcgtagt
     9661 cggtatggca gtgatggggc gcaacctagc gctc
//

</pre>