<pre>LOCUS       KJ739597                9783 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  UNVERIFIED: Escherichia coli strain 2533-54 O-antigen gene cluster,
            complete sequence.
ACCESSION   KJ739597
VERSION     KJ739597.2
KEYWORDS    UNVERIFIED.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9783)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9783)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Baranzoni,G.M.,
            Swimley,M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
REFERENCE   3  (bases 1 to 9783)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Baranzoni,G.M.,
            Swimley,M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
  REMARK    Sequence update by submitter
COMMENT     GenBank staff is unable to verify sequence and/or annotation
            provided by the submitter.
            On Dec 18, 2014 this sequence version replaced KJ739597.1.
            
            ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: 45X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2533-54"
                     /serotype="O175:K-:H28"
                     /db_xref="taxon:562"
     misc_feature    1..9783
                     /note="O-antigen gene cluster"
     gene            73..1203
                     /gene="wzy"
     CDS             73..1203
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AIG56909.1"
                     /translation="MNRQNLLIGVVFFSTLKVASILGNVFLSFLIPVILSSFALLVGW
                     EKKLIGRHSLFIIAVAFLILFSLIISLTIGHFGFASYLLSSYVMIITYVLIVGMFLVQ
                     SRDILSRPHFWIKTTKIVILFHTLLLSMQIVAFYFFNTLLDVGALTGGAETRSIVILS
                     DSLRLFRGGGLFEEPSIYVGFIFPFICLRYINNGNKIDYIIMLGLGGVIVTLSIVGCV
                     LVILFFIVVFFRFNIKLLLILTMLLLVAYYYSSDYLLFRLDKVLNGNDGSSSVKLSML
                     VDYFNNDVFFKGYGLLRKSDFNGAYDGLGDTTVYTSLFTIFGFPIGLLLIIPLLYKII
                     TMDYGIRQKVLLSLCFVKLASPTYMFFWALYLIVCLVGKNNK"
     gene            1200..2276
                     /gene="pglJ"
     CDS             1200..2276
                     /gene="pglJ"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylgalactosamine-N,
                     N'-diacetylbacillosaminyl-diphospho-undecaprenol
                     4-alpha-N-acetylgalactosaminyltransferase"
                     /protein_id="AIG56910.1"
                     /translation="MKILIISTASGYGGSERTIEIICKGLIQKHHVVVFAENEIHCRN
                     LKNNKIMVKTASRGNSIFATINNIQNIIKLANESDVIITNTNKSAFLIAISSIFSQNI
                     KSKRKLIFVRDFQWRYKKIIKRLLDNHIFCVASKAVESYVNKNFDKKVYIIPNPIDDV
                     SIDKLTTNEAFDIKEKFILCPAMISRWKGIHHILYAMYKSNILVKLYVVGDVVDLQYY
                     EELKQIVHRLGLSERVVFKGYTKNIQNYYRKCEFVISSSISRFGGPETFGRVIIESWQ
                     NEKTVIAFDCGGPSYLIDNEYNGILVEEGNDRELGDKINELLENEDKCKKMGYVGYLK
                     VKAEFSVNNIANEIIKLSSMRVDF"
     gene            2291..3340
                     /gene="mfpsA"
     CDS             2291..3340
                     /gene="mfpsA"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylfructose-phosphate synthase"
                     /protein_id="AIG56911.1"
                     /translation="MRLLIDGRMLHSSGVGVYIKQIIHELVTDSNENVTLILNETESV
                     EGIPEDIQIIKVNYGRFSIKNIFKLRKLLINYDCYLIPNLALTPFKSKKYKIFSTVHD
                     LCPVRMRDCFPLHLSIAYWILMLWQIINSNKIFTISGFTKKELKCFYPFFNENKYVVV
                     HNGWEKKNGNITFRGAEPSEIYGLCVGNVKKHKNILPLIDYLNNYVPDALIYIVGDYS
                     NFKTKAISDDPVSTKSIVFTGFISDEELDNLYKNASFFIFPSRYEGFGLPLLEAMAYQ
                     LPIFASNIEIFHEIAGEHIEYFDPYNFDGLNKMISSVQKKDLRKDYSDVLKKFSWKKT
                     VNSMLKVMNEYSFNK"
     CDS             3318..4475
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="AIG56912.1"
                     /translation="MNILLINKFYWPDVGGVETVVQQHANFLTTKGNKVTVLCIKKNA
                     SLKTEKINIQGVNIIRCSSLGTFFSMPLSLSFFYHYIRSVLDNKFIIFHEPFPLADIA
                     MCLFLPKKKKTMIFWHSDIVRQKKLKLILGFFIKKALRKANVVCTTSKNLSQHSEYLG
                     IVKEKLQIVPLSIDTDGLNKKVNSLNVNEFDSEIKSYADAQFISFGRLSYYKGLDVLL
                     EALKILKKEGVEPRVVISGKGELSNHIANIIKQEQLSNVIFINRFLKEDEKCFLLKNI
                     KCFLFPSIANSEAFGITQLESLALGTPVINTSLPTGVPDVSINNLTGLTVTPGDPYSL
                     AEAIKKIYLNKVNLNIFSVNAKIRASTIFDDKIVLPHFYNIILDVCNDKYI"
     gene            4487..5911
                     /gene="manC"
     CDS             4487..5911
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase 1"
                     /protein_id="AIG56913.1"
                     /translation="MSLIPVIMAGGTGSRLWPLSRELYPKQFLRLSGDHTMLQSTILR
                     LESITSNSPIVICNEQHRFIVAEQLRMVNRLKNNIILEPKGKNTAPAIALAAIAAKKI
                     YGEDSNLLILSADHIINNNDEFCDAVNNSIILSDKGNLVTYGIVPTKPETGYGYILRG
                     DLLSSTDQRTAFKVERFVEKPNMETAQAYVASGEYYWNSGMFLFRAGRYLEELKKYRP
                     DILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAGWSDV
                     GSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTKDAVL
                     IADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITVKPGE
                     GLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKIPLDL
                     IEVRSGSYLEEDDVVRLSDRYGRI"
     gene            5987..7372
                     /gene="manB"
     CDS             5987..7372
                     /gene="manB"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="AIG56914.1"
                     /translation="MMVCEMTKLNCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPK
                     TIVLGGDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTA
                     SHNPMDYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDATKRGRYQQINLRDAY
                     VAHLFGYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNF
                     PNGIPNPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLA
                     EAFLEKNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEM
                     SAHHYFRDFAYCDSGMIPWLLVAELVCLKEKTLGELVRDRMEAFPASGEINSKLAQPV
                     EAINRVEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESNANRELMIA
                     KTTEILKLLQL"
     CDS             7428..8255
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="AIG56915.1"
                     /translation="MVIINITTIQSRLDLCSATLWSLINQNHKPDKIILWISKDAYLL
                     DTGVLSLPQSVLKLMKIEKNLEIRYVTNIGPYRKIIPALREFSEEDILIYADDDVIYS
                     PLWLHELMITFDKYMGEYPVASRVRKINKNFFGAVKGYIRFPLIEYESIIEDNFIITG
                     VGGCVLKKKHIKHEMIYDDSFIDVAPRTDDLWLSKILQLSNTKIVVCPAALPHLNEIS
                     HDSYALNTLNNNEHKNSNVIMKGIRKFKSLFLAELGFATCNNDIMLRSIDDHFHGIK"
     gene            8257..9663
                     /gene="wzx"
     CDS             8257..9663
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AIG56916.1"
                     /translation="MSLISNIKNTSIAQVVKIICQLTAILVFAKLLTPTEFGVVAMAT
                     VVINFANIIRDIGTNAAIISRDTISHQLKSTLFWVNFIIGITLFSIIALSSELIAAYF
                     NNQHLRLLLIVLCLSFPLNSVSSVHLALLERESKFSTIAKVEIFSSVISLIVAIVAAA
                     NHFGAYSLVIQTLLYSLLSSIFFIRYSKWSPVFVFDFNELKSIFNFSSNIVGFNFINY
                     FSRNSDQIIIGKIYGPELLGNYSLAYRMMLFPLQSITFVLTRSLFPIISRIKDDKIES
                     GKAYLRVLTIICLIVPPLMIMLSLLSFDVISLFFPGKWDMVPNMLMWLAPTAILQSMI
                     STTGSIFMAFNRVNFLLKISIFNCALQMSAFLLGSFFDVYVLLQFYFIANLIMFLPNV
                     MSALSLVGLRYSIFLKQMIVCLVPSGILFFILSLLSMVLCRNTVLNLFLKCSLSFLVY
                     IIAVFPLRNVFKNNVKCL"
ORIGIN      
        1 tgagggaaat cgcagcgtac tggtaactgt caagccaggg gcggtagcgt gcctttttaa
       61 agatgatata ctatgaatcg acaaaattta ctcatcggtg tggtgttttt ttccacactt
      121 aaagttgcta gcatattggg taatgttttt ttaagttttt taataccagt aattttatct
      181 agttttgctc tcctcgttgg atgggaaaag aaacttattg ggagacattc tctctttata
      241 attgcagtcg catttctaat tcttttttca ctaataatta gtttaacaat aggtcatttt
      301 gggttcgcaa gctacctgct ttcctcatat gttatgataa tcacatatgt attgatagtg
      361 ggtatgtttt tggtacagtc aagagatata ctttctcgac ctcatttctg gataaaaaca
      421 acaaaaatag ttatcttatt tcatacgcta ctattatcta tgcaaatagt agcattttac
      481 ttcttcaata ctttattaga tgtaggggct cttactggtg gtgccgaaac aagaagtatc
      541 gtgatattat cagactcttt aaggcttttt cgcggtgggg gcctgtttga agaaccttca
      601 atttatgtag ggtttatatt tccattcata tgcttgcgtt atataaacaa tggaaacaag
      661 attgattata ttatcatgtt aggcttgggg ggggtcattg taacgctatc aattgtagga
      721 tgtgttcttg ttatattgtt ttttattgtt gtttttttta gatttaatat aaaattatta
      781 ttaattctta ctatgttatt gcttgttgca tattattatt ctagcgatta tcttctgttt
      841 agacttgata aggtcttgaa tggaaatgat ggaagcagtt ctgtcaaatt atcgatgctt
      901 gtagattact ttaataatga tgttttcttt aaaggctatg gtttacttag gaaaagtgac
      961 ttcaacggtg catatgatgg tttgggggat acaacagttt atactagtct gtttactatt
     1021 tttggcttcc caattggcct gcttcttatt ataccactac tttataaaat cataactatg
     1081 gactatggga taaggcaaaa ggttttattg tctttatgtt tcgttaaatt agcatctcct
     1141 acttatatgt ttttctgggc tttatactta atagtctgcc ttgtgggaaa aaataataaa
     1201 tgaaaatttt aatcatctca actgcatctg gatatggtgg aagtgaaaga accatcgaga
     1261 ttatttgtaa gggtttgatt caaaagcatc atgtggttgt tttcgctgaa aatgaaattc
     1321 attgtaggaa tttaaagaat aataaaataa tggttaaaac agcaagtaga ggtaattcaa
     1381 tttttgcaac tattaataat attcagaata taatcaagtt ggcaaatgaa agtgatgtta
     1441 taattacaaa tacaaataag tcggcttttt taatagcaat ctcttcaata tttagccaaa
     1501 atattaagag taagcgtaaa ttgatttttg ttagggattt tcagtggcga tataaaaaaa
     1561 taattaaacg attgctcgat aatcatattt tttgcgttgc atctaaggcc gttgaaagtt
     1621 acgtcaacaa aaatttcgat aaaaaagttt atataatccc taacccgata gatgatgttt
     1681 caattgataa gctaacaact aatgaggcat tcgacattaa agaaaaattt attctctgtc
     1741 cagctatgat ttcacgatgg aaagggattc accatatttt atatgcgatg tataaatcca
     1801 atattttagt aaagttatat gtcgttggtg atgttgtcga tcttcaatat tatgaagaac
     1861 tgaaacaaat tgtgcatcga ttaggattaa gtgagcgtgt tgtttttaag ggatatacta
     1921 aaaatattca aaattattat cggaaatgtg agtttgtgat tagttcatct atttctcgtt
     1981 ttggaggtcc tgaaaccttt ggaagggtaa ttattgagtc ttggcagaac gaaaagactg
     2041 taattgcatt cgattgtggc gggccaagtt atctaatcga taacgaatat aatgggatac
     2101 tggttgaaga aggaaatgat agggagttag gtgataaaat caatgagcta ttggaaaatg
     2161 aagataaatg caaaaaaatg gggtatgtgg gttatttgaa agttaaagct gagttttcag
     2221 ttaataacat agctaacgaa attataaaac tatcgagtat gagagttgat ttttaagttg
     2281 gagagttatt ttgcgtctat taattgacgg aagaatgtta cattcttctg gtgttggtgt
     2341 gtatatcaaa caaattatac atgaattggt cactgattct aatgaaaatg tgactttaat
     2401 acttaatgaa acagaaagtg tcgagggtat acctgaagat atacaaataa taaaagttaa
     2461 ctacggtagg tttagtatca agaacatatt caaacttcgg aagttgttaa ttaactacga
     2521 ctgttattta atcccaaatt tagctttaac gccttttaaa agcaaaaaat ataaaatttt
     2581 ttcaacagtg catgatttat gcccagtacg gatgagagat tgttttccgc tgcacctatc
     2641 tatagcctat tggatcctta tgctatggca aataattaat tcaaataaaa tttttacaat
     2701 ttccggtttc accaaaaaag aattaaagtg tttttacccc ttttttaatg aaaataaata
     2761 tgttgtagtt cataatggat gggaaaagaa aaacgggaat attacatttc gaggggcaga
     2821 accgtctgaa atatatgggt tgtgtgtggg caatgtaaag aaacataaaa acattttacc
     2881 tttaatagat tatctaaata attacgtacc agacgcatta atctatatag tgggagatta
     2941 tagtaatttt aaaactaagg ctatttctga tgatccagta agtacaaaat ctatagtttt
     3001 tactggtttt attagtgatg aagaacttga taatttatat aaaaatgctt cgtttttcat
     3061 ttttccatcg agatatgaag ggttcggttt gccactactg gaagctatgg catatcaatt
     3121 gccaatattt gcgtcaaata ttgaaatttt ccatgagatt gcaggggaac atatagaata
     3181 ttttgacccg tataattttg atggattgaa taaaatgatt agttcggttc aaaaaaaaga
     3241 tctgaggaaa gattattctg atgtattgaa aaaattttcc tggaaaaaaa ctgttaatag
     3301 catgcttaag gtgatgaatg aatattcttt taataaataa gttttattgg cctgatgttg
     3361 gaggcgtaga aacagtagtc caacagcacg ctaatttttt aactacgaaa gggaataaag
     3421 ttacagttct ttgtattaaa aaaaatgcat cactgaaaac tgaaaaaatc aatatacaag
     3481 gagttaatat tattagatgt tcatcattgg gtactttttt ttcgatgccg ctatctctat
     3541 ctttttttta tcattatatt agatcggtgt tagataataa atttataatc tttcatgaac
     3601 catttccatt ggctgatata gcaatgtgtt tatttttgcc gaaaaaaaag aaaacaatga
     3661 ttttttggca tagtgatatc gtaagacaaa agaagctcaa acttatttta ggttttttta
     3721 taaaaaaagc attacgtaaa gcaaatgttg tttgtacaac atcaaagaat ttatctcaac
     3781 actccgagta tttgggaata gtaaaggaaa aattacaaat tgtaccattg tcaattgata
     3841 ctgatggctt gaataaaaaa gttaattctt taaatgtaaa cgaatttgat tctgaaataa
     3901 aatcttatgc tgatgcacag tttatatcat ttggtcgttt atcctattac aagggattag
     3961 atgtattact tgaagcatta aaaattttaa aaaaagaggg ggttgaaccg agagttgtaa
     4021 tttcaggtaa aggtgaatta tctaatcata tagcaaacat cataaaacaa gaacagcttt
     4081 ctaatgtcat tttcattaac agatttctta aagaagatga gaagtgtttt ttattaaaaa
     4141 atataaaatg ttttttattt ccttctattg caaattcaga ggcctttggc ataacacagc
     4201 tagaatcctt agccctaggg actcctgtca ttaatactag cctcccaact ggagttccgg
     4261 atgttagtat caataattta actgggctta ctgttacacc gggggatccg tatagtttgg
     4321 cagaagcgat taaaaaaatc tatttgaata aagtaaactt aaatatattc tctgttaatg
     4381 cgaagattag ggcatcaacg atttttgatg ataaaatagt acttccgcat ttttataata
     4441 taatattaga cgtttgtaac gacaaatata tttaatagga gcggttatgt ctttaatacc
     4501 agtaataatg gctggaggta ctggtagcag actttggcca ttatctcgcg agttataccc
     4561 aaagcagttt ttgagattat caggcgatca tacaatgcta caatcaacaa tacttcgttt
     4621 ggaaagtata acaagtaatt caccaattgt tatttgtaat gagcaacata gatttatagt
     4681 tgcagaacaa ttaagaatgg taaacaggtt aaaaaacaat attatcttag agccgaaagg
     4741 gaaaaatact gcacctgcta ttgccttagc tgctatagca gctaaaaaaa tttatggtga
     4801 agattctaat ttattaatat tgtcggccga tcacattata aataataatg atgagttttg
     4861 tgatgctgta aataattcaa taatactctc tgataaaggt aatttagtta cgtatggcat
     4921 tgtgcctaca aaaccagaaa ccggatatgg atatattctg cgtggtgatt tattaagttc
     4981 aactgatcaa cgaacggctt ttaaagtcga acgttttgtc gaaaaaccga atatggaaac
     5041 cgcccaggcc tatgtggcaa gcggcgaata ttactggaac agcggcatgt tcctgttccg
     5101 cgccggacgc tatctcgaag aactgaaaaa atatcgcccg gatattctcg atgcctgtga
     5161 aaaagcgatg agcgccgtcg atccggatct cgattttatt cgcgtggatg aagaagcgtt
     5221 tctcgcctgc ccggaagagt cggtggatta cgcggtcatg gaacgtacgg cagatgctgt
     5281 tgtggtgccg atggatgcgg gctggagcga tgtcggttcc tggtcttcat tatgggaaat
     5341 tagcgcccac accgccgagg gcaacgtttg ccacggcgat gtgattaatc acaaaactga
     5401 aaacagctat gtgtacgccg aatctggcct ggtcaccacc gtcggggtga aagatctggt
     5461 agtggtgcag accaaagatg cggtgctgat tgccgaccgt aacgcggtgc aggatgtgaa
     5521 aaaagtggtc gagcagatca aagccgatgg tcgccatgag catcgggtgc atcgcgaagt
     5581 gtatcgtccg tggggcaaat atgactctat cgacgcgggc gaccgctacc aggtgaaacg
     5641 catcaccgtg aaaccgggcg aaggcttgtc ggtacagatg catcatcacc gcgcggaaca
     5701 ctgggtagtg gttgcgggaa cggcaaaagt caccattgac ggtgatatca aactgcttgg
     5761 tgaaaacgag tcgatttata ttccgctggg ggcgacgcac tgcctggaaa atccggggaa
     5821 aattccgctc gatttaattg aagtgcgctc cggctcttat ctcgaagagg atgatgtggt
     5881 gcgcttgtca gatcgctatg gaagaattta atccattacg aatgaaatta agagaatcgt
     5941 tcattttaaa gcattcaact cttatattta ataaataaat ttttaaatga tggtgtgtga
     6001 aatgactaaa ttaaactgct ttaaagccta tgatattcgc gggaaattag gcgaagaact
     6061 gaatgaagat atcgcctggc gcattgggcg tgcctatggt gaatttctca aaccgaaaac
     6121 cattgtgtta ggcggtgatg tccgcctcac cagcgaaacc ttaaaactgg cgctggcaaa
     6181 aggtttgcag gatgcgggcg tcgatgtgct ggatattggc atgtccggca ccgaagagat
     6241 ctatttcgcc acgttccatc tcggcgtgga tggcggcatt gaagttaccg ccagccataa
     6301 tccgatggat tataacggca tgaagctggt gcgcgaggga gctcgcccga tcagcggtga
     6361 taccggactg cgcgatgtcc agcgtctggc agaagccaac gactttcctc ccgttgatgc
     6421 aaccaaacgc ggtcgctatc agcaaatcaa tctgcgtgac gcctacgttg ctcatctgtt
     6481 cggttatatc aacgtcaaaa acctcacgcc gctcaagctg gtgatcaact ccgggaacgg
     6541 cgcagcgggt cccgtagtgg acgccattga agcccgcttt aaagccctcg gcgcgcctgt
     6601 ggaattaatc aaagtacaca acacaccgga cggcaatttc cccaacggta ttcctaaccc
     6661 gctgctgcct gaatgccgcg acgacacccg caatgcggtc atcaaacacg gcgcggatat
     6721 gggcattgcc tttgatggcg attttgaccg ttgttttctg tttgacgaaa aagggcagtt
     6781 tatcgagggc tactacattg tcggcctgct ggcagaagcg ttcctcgaaa aaaatcccgg
     6841 cgcgaagatc atccacgatc cgcgtctctc ctggaatacc gttgatgtgg tgactgccgc
     6901 gggcggcacc ccggtaatgt cgaaaaccgg acacgccttt attaaagaac gtatgcgcaa
     6961 ggaagacgcc atctacggtg gcgaaatgag cgcccaccat tacttccgtg atttcgctta
     7021 ctgcgacagc ggcatgatcc cgtggctgct ggtcgccgaa ctggtgtgcc tgaaagagaa
     7081 aacgctgggc gaactggtac gcgaccggat ggaggcgttt ccggcaagcg gtgagatcaa
     7141 cagcaaactg gcgcaacccg ttgaggcgat taaccgcgtg gaacagcatt ttagccgtga
     7201 ggcgctggcg gtggatcgca ccgatggcat cagcatgacc tttgccgact ggcgctttaa
     7261 cctgcgcacc tccaacaccg aaccggtggt gcggttgaat gttgagtcaa atgcaaatag
     7321 agaacttatg atagcgaaaa ctactgaaat attgaagtta ctacagcttt aatatatttt
     7381 tctaagatta tgaatatata tttcaatcaa aaaatggaat tctttatatg gttataatta
     7441 atattactac aatacaatca aggcttgatt tatgttcagc aacattgtgg tctttaataa
     7501 accaaaatca taaacctgac aaaataatat tatggatatc taaggatgca tatttattag
     7561 atacaggagt gttaagcttg cctcaatcag tgctaaaact tatgaagatt gagaaaaacc
     7621 tggaaataag atacgttaca aatattgggc catatcgaaa aatcattcct gcattaagag
     7681 aattttccga ggaggatatt ctgatatatg cagatgatga tgtaatatat tcaccactat
     7741 ggttgcacga attgatgata actttcgata aatacatggg tgaatatcct gtagctagtc
     7801 gagtaagaaa aataaataaa aatttctttg gagctgtaaa gggatatatt aggtttccat
     7861 taatagaata tgaaagtatt attgaagata attttattat aacgggggtt ggaggctgtg
     7921 tattgaaaaa aaaacatatt aaacatgaaa tgatatatga tgattcattt atagatgttg
     7981 cccctcgtac agatgactta tggcttagta aaatccttca gctatctaat acaaagattg
     8041 ttgtatgtcc agcagcatta ccacatctga atgaaatatc acatgattct tatgccctta
     8101 atactttaaa taacaatgag cacaaaaatt ctaatgtaat tatgaaaggg attcgtaagt
     8161 ttaagagttt atttttagct gagttgggct ttgcaacgtg taataatgac attatgcttc
     8221 ggagtattga tgatcatttt catggaataa aatgatatgt ctttgatttc aaatataaaa
     8281 aacacatcaa ttgcacaggt ggtaaaaata atatgtcaat taactgctat tctagttttt
     8341 gcgaaactat taaccccaac agagtttggg gttgtcgcta tggcaacagt agttatcaat
     8401 tttgctaata taattagaga tataggtaca aatgcagcaa taataagtag agatacaatt
     8461 agtcatcaat tgaaaagtac tcttttctgg gttaatttta taataggtat cactctattt
     8521 tcaataattg ctttatcttc tgagttgatc gctgcttatt ttaataatca acatttacga
     8581 ctgttgctta tcgttctttg tttgagcttt ccattaaata gtgtttcaag tgtgcattta
     8641 gccttgttag agagagaatc aaaattctct actattgcga aggttgaaat attttcatct
     8701 gtaatttcat taattgtggc gatagttgct gctgctaatc attttggagc atatagttta
     8761 gtcatacaaa cgctacttta ttctttgtta agttccattt tttttatacg ttattctaaa
     8821 tggagtccgg tttttgtttt tgatttcaat gaattgaaaa gcatttttaa tttcagttca
     8881 aacatagttg gttttaattt tatcaattat ttctcaagaa atagtgacca gataataatt
     8941 gggaaaattt atgggccaga attacttggg aattactctt tagcatatag aatgatgtta
     9001 ttccctttgc aaagtataac atttgttctt acgcgttcat tatttcctat tatcagtaga
     9061 ataaaagatg ataaaattga atctggaaaa gcttatttaa gagtgttaac aattatatgt
     9121 ttaattgttc ctccgctcat gataatgtta tctttattga gttttgacgt aatatcatta
     9181 tttttcccag gaaagtggga tatggtcccg aatatgctca tgtggttagc accgacagcc
     9241 atactgcaat caatgattag tacgacaggc tcgatattta tggcatttaa tagagtcaat
     9301 tttttattaa aaatatcaat ttttaactgc gccttacaaa tgagtgcatt cctattaggt
     9361 agtttttttg acgtgtacgt tttattgcag ttttatttta tcgcaaactt gataatgttt
     9421 ttgccaaatg ttatgtctgc gctaagtttg gttgggctgc gctactcgat ttttctgaag
     9481 caaatgattg tatgcctagt tccatccgga atattgtttt ttatactttc tctgttaagt
     9541 atggttttat gtaggaacac ggttcttaac ttatttctta agtgctctct ttcatttctg
     9601 gtatatatta ttgcggtttt tccactaaga aatgtattta aaaataatgt gaaatgttta
     9661 taactatttt tattagtgtt atcgttccta tcgttttagt atcaccgcat tccccgcggt
     9721 aacccctgac aggagtaaac aatgtcaaag caacagatcg gcgtcgtcgg tatggcagtg
     9781 atg
//

</pre>