LOCUS DQ008592 5660 bp DNA linear BCT 07-OCT-2005
DEFINITION Escherichia coli serogroup O174 O antigen gene cluster, partial
sequence.
ACCESSION DQ008592
VERSION DQ008592.1
KEYWORDS .
SOURCE Escherichia coli
ORGANISM Escherichia coli
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
Enterobacteriaceae; Escherichia.
REFERENCE 1 (bases 1 to 5660)
AUTHORS Beutin,L., Kong,Q., Feng,L., Wang,Q., Krause,G., Leomil,L., Jin,Q.
and Wang,L.
TITLE Development of PCR assays targeting the genes involved in synthesis
and assembly of the new Escherichia coli O 174 and O 177 O antigens
JOURNAL J. Clin. Microbiol. 43 (10), 5143-5149 (2005)
PUBMED 16207976
REFERENCE 2 (bases 1 to 5660)
AUTHORS Beutin,L., Kong,Q., Feng,L., Wang,Q., Krause,G., Leomil,L., Jin,Q.
and Wang,L.
TITLE Direct Submission
JOURNAL Submitted (14-APR-2005) TEDA School of Biological Sciences and
Biotechnology, Nankai University, 23# HongDa Street, Tianjin
300457, P.R. China
FEATURES Location/Qualifiers
source 1..5660
/organism="Escherichia coli"
/mol_type="genomic DNA"
/db_xref="taxon:562"
/note="serogroup: O174"
misc_feature <1..>5660
/note="O antigen gene cluster"
gene 51..794
/gene="wbcX"
CDS 51..794
/gene="wbcX"
/note="glycosyltransferase"
/codon_start=1
/transl_table=11
/product="WbcX"
/protein_id="AAY28247.1"
/translation="MSTVTLVITSCGRFELLEKTISSLVNRYPFTEKIIIEDSGNVKV
INKIKEKYDRDFTILINEKNIGQIKSIDKAYSLVTTEYIFHCEDDWHFYRDGFIEDSL
DILKEYRHISMVSLRDWLNDVSINCHMERSTLLQTGNGTRFFMLKPKNGDGWGGYSFN
PGLRRLQDYKEIIGQFSKVGHEKNISLYFLEKGMNMALLESSAVEHIGWNHHILSKNN
PQERFYLLKKYLPKEVTNVLKIIYRKIAG"
gene 797..2068
/gene="wzx"
CDS 797..2068
/gene="wzx"
/note="O unit flippase"
/codon_start=1
/transl_table=11
/product="Wzx"
/protein_id="AAY28248.1"
/translation="MKYLFIWSLTPKLYELSLNLSIGIALINYLGPVEQGKIGFLINL
CTLMSFLTTLGIGPVYSNFVSRSNNERLISNKFKENVTLRFLGYILFLLVSLLFLFFF
KRGLIYLSIPFLIGKLFFSFDIYYNLIEGKAGFKNYAISKFISLTCINVFRLYCIYAQ
LDIYWVAFSFFLTDFLTFFVYFLLFDKFKYFGFKFDYKKSLIIFKINYKLALSTIVVS
LFTQLDIVMIGTMLGDKAAGEYYASTRLATPLVFISTIIISTFFSKLSRNWVVNKKEY
YELLAFISGSIIFSYSAIVLIIFIFGNDIFFLFFSSEYKASYDLFLIHIVGLIFVLLG
PLTGKHLIIKKDYGAELSKTLLAAIVNIALTSIAVLKYENLNLVAVSTLISYMIANFG
YFIVKKDWLLIKAILNGVNPLFLVRYAKKIL"
gene 2049..3188
/gene="wzy"
CDS 2049..3188
/gene="wzy"
/note="O unit polymerase"
/codon_start=1
/transl_table=11
/product="Wzy"
/protein_id="AAY28249.1"
/translation="MLKRYSNLIFSFVIAFSFFSYVMNPSLKFIYKSPVINFIPFLLC
CLITIIFLTEKKIKLNYYELFSILYVGFFFILQYTYFIQSTDSIETLLRLMSVNFSFL
FGLLLGWCFKRESIEKLFILWVLLLSIANICGVINYSDGVEFNHLNFTLPLGTVVTWL
IFKAFMKDTDRYIFTLSVILFLFFNIIFAGSRTAIFLPILVSLFIMVIFRKYVSIKKT
VISFIILITITIISLPYILSNLNAYFLSKVQNMADISEDSRYNLYLKCFNMLLEHPLG
IGYGNYKYFITEPYPHNILLEIGLNSGVLGCFVFITYVMITTIVIIKKTKSCYNEKNL
FVLTIFLYSLFSWMFSNDFASSSVVFFLLGVLGHIASSNNEKEIK"
gene 3191..4075
/gene="wbcY"
CDS 3191..4075
/gene="wbcY"
/note="glycosyltransferase"
/codon_start=1
/transl_table=11
/product="WbcY"
/protein_id="AAY28250.1"
/translation="MFSIVITTKDRAYYLKRAVDSILKSTISPKDIVIINDGGKAICE
NDFPPMKHITLSIVNNRFSMGANYSRNQGIDKALTNYVFLLDDDDAFTPTTFENRINI
IKSSVDIGVVFTGINIVSSKNLDKVIRKSKNINDQITTHQLLTEGNVIGSTSRALIRK
DLFCEAGRFDPQLSCLQDYDLWIRMSLVSRIVNDHKYGVYYTIHDNGRQISTNYEKYM
QVGKLLINKYDSLLNKNTINAFKSNIYLRVAISASASSNKARLKYSFMSLKYRLNIKA
LALFLFPSSVLKRFYNYV"
gene 4661..5482
/gene="wbcZ"
CDS 4661..5482
/gene="wbcZ"
/note="glycosyltransferase"
/codon_start=1
/transl_table=11
/product="WbcZ"
/protein_id="AAY28251.1"
/translation="MKGFSVLMSVYKGEKENYLDECFKSLHEQTIKANEIILVIDGPV
SKELYQVIDKWGEVLPIIKVKLDKNVGLGQALNIGLKHCNFELVARMDTDDYCVKDRF
LLQMKFFEEHNDIMVLGGEIEEYDQSLSIPLGKRTTALSHEEIIELAKKRNPLNHMTV
MYKKSFILSVGGYQHHLYMEDYNLWLRVLASGGCICNLPKVLVHVRAGEEMIKRRKGW
IYIKSEIQLARLKSKLNITCFWNNYYTMTLRILARLMPTPLLKFVYSKLRTSKLA"
ORIGIN
1 attggtagct gtaagccaag ggcggtagcg taaacatttg agaggaatta atgagtacag
61 ttacactcgt tataactagt tgcggtagat ttgaattatt agaaaaaaca atatcttctc
121 ttgtaaatag gtacccattt acagagaaga tcataattga ggattctggt aatgtaaaag
181 ttataaacaa aataaaagaa aaatatgata gggattttac aatcttaata aatgaaaaaa
241 acattggaca aattaaaagc atcgataagg catatagttt agtcactacc gaatatatat
301 tccattgtga agatgattgg catttctata gagacggatt catagaagat tcattagata
361 tattgaaaga gtatagacac atttcaatgg tatcacttag agattggcta aatgatgtat
421 cgatcaattg tcatatggaa agatccacgc tcttacaaac gggaaatgga acaagatttt
481 tcatgttaaa acccaaaaat ggtgatggct ggggagggta ttcgttcaac ccggggctga
541 gacgacttca agattataaa gaaataatcg gacaattttc aaaagtaggg catgaaaaaa
601 atataagttt atatttttta gaaaaaggaa tgaatatggc gctattagaa tcttcagctg
661 ttgagcacat aggttggaat caccatatat tgagtaaaaa taatcctcaa gaaagatttt
721 atttgcttaa aaaatattta ccaaaggaag taactaatgt gctcaagata atttatagaa
781 aaattgctgg gtgattatga aatatctttt tatttggtct cttactccca aattatatga
841 attaagcctt aatctttcta tcgggatagc tcttataaat tatctaggac ctgtagaaca
901 aggtaaaata ggttttttaa ttaatttatg cacattaatg agttttctta ccacgcttgg
961 aattgggcct gtatattcta attttgttag tcgttcaaat aatgaaagat taatatccaa
1021 taaatttaag gagaatgtta cattaaggtt tttaggttat attttgtttc ttctggtttc
1081 acttttattt ctattcttct ttaaaagggg gttgatatat ttatcaatac catttttaat
1141 agggaaatta tttttcagtt ttgatatata ttataacctc attgaaggta aggctggatt
1201 taaaaattat gcaatttcaa aatttatatc tttgacatgt ataaatgtat ttcggttata
1261 ttgtatatat gcacaattag atatatattg ggttgcgttt tcattctttt tgactgattt
1321 cttgacattt tttgtgtatt ttttgttatt tgacaaattt aagtatttcg gttttaaatt
1381 tgattacaaa aaatcattaa ttatatttaa gataaactat aagttagcat tatctacaat
1441 agttgtaagt ctctttactc agctggatat agtaatgatc ggaactatgc ttggagataa
1501 agctgcaggt gagtattatg catcaactag actcgctact cctttagttt ttatatcaac
1561 aattattata agcacttttt tttcaaaact ttctagaaac tgggttgtta ataaaaaaga
1621 atattatgaa cttttagctt tcattagtgg aagtataatt ttttcgtatt cagcaattgt
1681 actcattatt tttatatttg gaaacgatat atttttcttg tttttcagtt cagaatataa
1741 agcctcttat gatttattct taatccatat tgtaggtttg atttttgtac ttttaggccc
1801 gcttactggg aagcatttaa taattaaaaa agattatggg gcagaactta gcaaaacctt
1861 attggcagca attgttaata tagccttaac ttccattgca gtgttaaaat atgaaaatct
1921 taacttagtg gccgttagta cattaataag ttatatgatt gctaattttg gttactttat
1981 agtaaaaaaa gattggctat taatcaaagc tatattgaat ggtgttaatc cccttttttt
2041 ggtgagatat gctaaaaaga tactctaacc ttatattttc ttttgttatt gcgttttcct
2101 ttttttcata cgtaatgaat cccagtttaa aatttatata taaatcacca gtaataaatt
2161 ttatcccttt tttgttatgt tgtttgataa caatcatttt tcttacagag aaaaaaatca
2221 agcttaacta ttatgaatta ttttcaattc tttatgttgg gttctttttt atacttcaat
2281 atacctattt tatacaatcg acagattcaa tagaaacttt actacgatta atgtcggtaa
2341 atttttcatt cttatttgga ttactattag gctggtgttt taaaagggag tcaattgaga
2401 agctttttat actatgggtc ctattacttt ctattgctaa tatctgcggt gtaatcaatt
2461 attctgatgg ggtggaattt aatcatttga actttacatt acctctaggg actgttgtta
2521 catggttaat ttttaaagca tttatgaaag atactgatag gtacatattc acattatctg
2581 ttatattatt tttatttttt aatataatat ttgccggaag tcggactgct atttttctac
2641 caatcttagt aagtttattt attatggtga tatttagaaa atatgtgtct ataaagaaaa
2701 cagtgatctc attcattata ttgataacaa taactataat atcattgcct tatattttaa
2761 gtaacttgaa tgcttatttt ttaagcaagg ttcaaaatat ggctgatatc agtgaggata
2821 gtcgttataa tttatatttg aaatgtttta atatgctttt agagcatcct cttggaatag
2881 ggtatggcaa ttataaatat tttatcactg aaccttatcc tcataatatt cttttagaaa
2941 ttgggctgaa tagtggggtt ttaggatgtt ttgtgtttat aacctatgtg atgatcacca
3001 caatagtcat aataaagaaa acaaaatcct gctataatga aaaaaatcta tttgttttga
3061 ctatattttt atattcactt tttagctgga tgttttcaaa cgattttgca agtagcagtg
3121 ttgttttttt cttattgggt gtgctaggtc acatagcctc tagtaataat gaaaaagaga
3181 ttaaataaat atgttttcta tagtaattac aactaaagac cgggcttatt atttgaaaag
3241 agcagttgac agcatattaa aatcaactat ttctccaaaa gatatcgtaa taattaatga
3301 tggtgggaag gctatatgtg aaaatgattt tccacctatg aaacatatta cattaagtat
3361 agttaataat cgtttttcaa tgggagctaa ctattcaaga aatcaaggaa ttgataaagc
3421 cttaactaat tatgtatttt tacttgatga tgatgatgcc tttactccaa ctacctttga
3481 aaatagaatt aatataatta aatcttcagt ggatattggg gtcgttttta ctggaattaa
3541 tatagtatca tccaaaaatt tggataaagt aattagaaaa tcaaaaaaca tcaatgacca
3601 aatcactacg catcagcttc taacagaagg caatgttatc ggatctacat cacgtgcatt
3661 aattcgtaaa gatttattct gtgaagcggg aagatttgat cctcaattaa gctgtcttca
3721 ggattatgat ttatggatta gaatgtcatt ggtatcaaga attgtcaatg atcacaaata
3781 cggagtttat tatacaatac acgacaatgg gcgacaaatt agtactaatt atgaaaaata
3841 tatgcaagta ggaaaattgt taataaataa atatgattct cttcttaata aaaatacaat
3901 taatgctttt aagtctaata tttacttaag agttgcaata tctgcctctg cttcatcaaa
3961 taaagcaaga ttaaaatatt cttttatgtc attaaaatat aggcttaata ttaaagcttt
4021 agctttgttc ctattcccta gctctgttct taaaagattt tataattatg tataagtttg
4081 aatgagtaat tgcaatatta ttacgttttc tgtatttaaa ctcaagattt tttcttttca
4141 cctttctttt tgctaggcac ttgttgaata gtagatggat gtgtaataca ataatggaag
4201 atgtcttagt gatttataca tttcaataaa ctttcaaaaa aagttaatta taacgcatat
4261 atttaaatga tttatattta atgtttgtta gtcagataaa tattttattg tctctatagc
4321 tataggcttt tgttactttt aaataaagaa gatatgtgaa aaataaacaa ttttaatgaa
4381 gagtggagcc atgtgattca tgtgttctaa tttttatctt aataacaatg atgtacaatg
4441 gcatggttac aatactattt aatatggtag gggtagtatc tttgaagtag tttatattaa
4501 aaactatgtt cgtaaattat atatgttccg acaatatata aagattttag atagagggat
4561 tatctagtaa tacaatgcgt tagataaaat aaatccgtat ctctattact aaaacaatta
4621 aatgttttat taaagacaag gatattaaga gggcatatta atgaaaggtt tttcggtact
4681 tatgtctgtt tataaaggtg aaaaggaaaa ctatcttgat gagtgtttta aaagcctgca
4741 tgaacagaca attaaagcta atgaaatcat tttggttatt gacggcccag tttccaaaga
4801 attatatcag gttatagata aatgggggga ggttctgccc attataaaag ttaaacttga
4861 taaaaatgta gggcttggac aagctttaaa tataggcctt aagcactgta attttgagtt
4921 ggttgcacga atggatactg atgattattg tgtaaaggat agatttttat tacagatgaa
4981 attcttcgaa gaacacaatg atataatggt cttggggggc gagatagagg aatacgacca
5041 atccttgagt attccattag gcaaaagaac aacagcatta tctcacgaag aaataattga
5101 acttgcaaaa aaaagaaatc ctctcaacca tatgacagtc atgtataaaa aaagctttat
5161 cttaagtgtt ggaggttatc aacatcattt atatatggaa gattataatc tttggttgag
5221 agtattagct tctggtggtt gtatttgcaa cttacctaag gtattagtgc atgtaagagc
5281 tggggaagaa atgatcaaaa gacgaaaagg ctggatatat atcaagagtg aaatacagct
5341 agcacgttta aaaagtaagt taaatataac ctgtttctgg aataactact atacaatgac
5401 acttaggatc cttgccagac taatgccgac gccacttcta aaattcgtat attctaaact
5461 tagaacgtct aaattagctt gatgttgaca tttcatttgt ggggagggtg aattaattca
5521 tttaaaatga taaaacattg cgccaaatgc caataaaaag ttatattttt acacggaatc
5581 tgaagtaacc tttatagttc atcccctgac aggagtaaac aatgtccaag caacagatcg
5641 gcgtcgtcgg tatggcagtg
//