<pre>LOCUS       KJ739598               11895 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  UNVERIFIED: Escherichia coli strain 198 O-antigen gene cluster,
            complete sequence.
ACCESSION   KJ739598
VERSION     KJ739598.2
KEYWORDS    UNVERIFIED.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11895)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11895)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Baranzoni,G.M.,
            Swimley,M., Burrell,A.M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
REFERENCE   3  (bases 1 to 11895)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Baranzoni,G.M.,
            Swimley,M., Burrell,A.M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
  REMARK    Sequence update by submitter
COMMENT     GenBank staff is unable to verify sequence and/or annotation
            provided by the submitter.
            On Dec 18, 2014 this sequence version replaced KJ739598.1.
            
            ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: 45X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="198"
                     /serotype="O171:K-:H2"
                     /db_xref="taxon:562"
     misc_feature    1..11895
                     /note="O-antigen gene cluster"
     gene            116..736
                     /gene="nnaD"
     CDS             116..736
                     /gene="nnaD"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate 5-dehydrogenase"
                     /protein_id="AIG56927.2"
                     /translation="MKKKLIIIGAGGFAKAVIDSLDHNEYALEGFVDSLKSGMHQGYP
                     IVGHSLSDISLPTEYYYFIAIGDPDDRALWLSQIRDLKLETINVIDKTAIVSTRSSIG
                     TCIYIGKMAIVNCDSQLEDGVVINTRALIEHGNHISYCTNISTNVVLNGDVYVGEKSF
                     IGSCTVVNGQIKIGSSTIVGSGSVVIRNIPNNVVVAGTPTRLIRER"
     gene            741..1781
                     /gene="nnaB"
     CDS             741..1781
                     /gene="nnaB"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="AIG56926.1"
                     /translation="MSKIYIVAEIGCNHNGDFNLAKKMVEEAKKAGVDAVKFQTFKAD
                     QLISKYAPKAEYQIKVTGNQESQLEMTRKLELPYEEFIKLEEYAKELGLDVFSTPFDF
                     ESIDFLASRNQKMWKIPSGELLNLPYLEKISRLPIEGKEIVLSTGMATIEEIKASINV
                     LTKNGMAPEKITILHCNTEYPTPYEDVNLNSIIGLKNEFKGYNVGFSDHSPGYYAAIA
                     SVPYGITFIEKHFTLNKDFEGPDHKASVTPDELCLLCEGIRAVEKSLGSHIKIVTKSE
                     RKNKIVARKSIVAKRNIKKGEIFTIDNITTKRPGNGISPMHWYDILGKKAEKDFEEDQ
                     LIKYSGFKEQEV"
     gene            1785..3050
                     /gene="nnaC"
     CDS             1785..3050
                     /gene="nnaC"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="AIG56925.1"
                     /translation="MSRKKIAIIPARAGSKGLPDKNVLMLLDRPLIAYTIEAAVSSNL
                     FDKVIVSTDSERYKTIAEYYGAEVMLRSAELSSDTATSYMVIEDVLKNNPGYEIIALL
                     QPTSPFRTANHIIQSMELFEETDYARFLVSVSECFASTDLIKPLAQNGSLENFDKDFS
                     NYRRQNCRQYSPNGAIFLAYVSDYLEQKHFFGNKSVAFIMNKEDSIDIDDHLDFCTAI
                     AIQSNKSKINSIQNIIHQRINEKKEKFELCYPITLIGHSIFDYWDIECLNGQRVNNLG
                     IAGINSEQYYTLILESGLIKSIGNIILLMTGTNDLLNENWTPNYTLYWINKTIHFILH
                     NNPNAHIYFLATPPVRDRIDRSNSSIKYLNKILEENIKHTPCVTWVPLCDDFYDEFDY
                     LKIEFTTDGVHFNKIAYQYLENYIKGIIK"
     gene            3047..4201
                     /gene="nnaA"
     CDS             3047..4201
                     /gene="nnaA"
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid biosynthesis protein P7"
                     /protein_id="AIG56924.2"
                     /translation="MKKILYVTGSRAEYGIVKRLLAALKNDQDIELTIIATGMHCDEK
                     YGLTYKQIEKDGFVIEQLINININTLTNSDIINSMSVCQQKFGHYFQNNKFDAVMLLG
                     DRYEIFAVAVAAAIHNLPIIHIHGGEKTLGNYDEFIRHSITKMARLHLASTEQYRKRI
                     IQMGECPNSVVNIGALGAENALLMDLPSKSELEKKYGALTQPYFIVVFHPETLSQTST
                     TKQISELLTALRELTESYNFIFIGSNSDTGSDEIMKEILRFCQLTNSRLFKSVKPEEY
                     LALNKYSQGLIGNSSSGLIEIPSLLVPTINIGNRQKGRVRGTTVIDCACSAETIKSAI
                     LLSQGADFQRSLQFAENPYYKKAVLESAVNVIKSFVFNEKEHYKDFYDLD"
     CDS             4724..5377
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DDE domain protein"
                     /protein_id="AIG56923.2"
                     /translation="MRDCHSSGDKDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHT
                     LVIGQIKTDEKSNEITTIPELINILDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAV
                     KGNQGRQNKAFEERFPLKELNNTKHDSYAISEKSHGREEIHLHIVCGVPDELIDFTFE
                     WKGLKKLCVAVSFRSIIAEKKKEPEMTDRYYISSADLTAKKFATAILNHWLVENKLH"
     gene            5782..6843
                     /gene="wzx"
     CDS             5782..6843
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AIG56922.2"
                     /translation="MSLMGMGLIAVNWSFDGAIARYYYRYGKFNILNVYYTAIVLSLA
                     LTFVLCLVASFFNNSIYLYAVVCAFLQGVFNILSTFQQCQKNAFKYIAIQFTFSIIST
                     ALTIVLVLKWHADIDSRIVAILVGLSCAIFIGMKFLHVYRKIHVKDIIKKLNFTYLLS
                     FGLPLIFHQISLMIKGQLDRIIIATTFDYVQLAHYTAGYQVASVISVGIMAVNKAIVP
                     TYYEACKSNKINKNKIYFLFWLLLPICLLPSIITYFIPPVFFVALLGEKYEFIGTYVT
                     IFTFGFMLQIPYLLLVNYLFYLNKTRVIAKITILSSIVHIVSIYILKSYGIVYIPWAL
                     VLSNVFCIFLLYFYMGRKD"
     gene            6843..7793
                     /gene="lst"
     CDS             6843..7793
                     /gene="lst"
                     /codon_start=1
                     /transl_table=11
                     /product="CMP-N-acetylneuraminate-beta-galactosamide-
                     alpha-2,3-sialyltransferase"
                     /protein_id="AIG56921.2"
                     /translation="MNVFICFTPFQLFLSLEIVKLENLPPSRCIFFLYNYHDRFKVKK
                     NYIEVTKGFEQVYYESRPLTLAFIFKLRSIAKQIGQVKTAYLASIDNNIAHYLLSFLK
                     PLSIRTFDDGTANITQMSLFYRDIKTNPLKAALHLLLGKKYNREKIKSASDLHYTVYR
                     GVNNITDKIEILEMLRENTSDVENRKVLKIMLGSIYSELVDCSEDEVYIQNYIHRQCI
                     DEEMLYIPHPREDLSRINVPFVSDWRISEQIIVDFISCGYEVYLYGFPSSTFFNLSSN
                     AHVKCCIFMTDKLREEFKDLIKIAKSLNMSITNIPTGSFK"
     gene            7790..8941
                     /gene="wzy"
     CDS             7790..8941
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AIG56920.2"
                     /translation="MIPHHNLICSFKLRLQIFFTLFTLYCIFSLWGMSVENKIFTYIS
                     LFISFLIVIFALTQGIRSVSFFILFAAFFCLSSMALFSNKTGEPVAILINAYLFVFAM
                     MISNIIYKLRLACRSSVILFSTTHIYFFIFCIYKYKIDGFINENTFNELFWQSSRNIV
                     SAILLLTTIFFLAVNYIEQKKIYLSVLFINMISSILLYGRSGVVLSFIVLFIGIYLKF
                     FKEEKNVIKIILFLFFSILILVLILYTGFTDNMVSAILSHTNLQSGFDTPRIQMWYDY
                     IVNFDYKILLLGTNFLFSPLITSFDGNPHNSFIMFQARFGIFAVLFLLSISYLYLKLF
                     QNSLLLSLLLGLIFVRMFFDTFSLFGYFDFIWVYMLIYLPRNNKKRSFL"
     gene            8938..9906
                     /gene="epsJ"
     CDS             8938..9906
                     /gene="epsJ"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase EpsJ"
                     /protein_id="AIG56919.2"
                     /translation="MRVILTLIIPVYNVEAYIEECLESLFPQIANSKNNDVEVIIVND
                     GTPDKSMDIVIHAIKKYSLERQVRVVNQCNQGLSAARNTGIRLATGDYIGFIDSDDYV
                     SENYIREIKSNILDLIISSGDILKFNFHFFRNKVSRSRRTNLINTVRYSGTEYIKNDI
                     KENSWYAWRRIYASHLFDDILFDVGKKYEDVLTVPLLTFKSSNCVALNSTLVFYRSNP
                     YGITKNSELEHAIDILSRVNDHVEIMKRRGYNKQLISLHVYNIAKTFVNTVATAWKGK
                     FDIFFIHSNFTTIYPHLNVFRTVYVYVYYFKAVLKYLLSQVKRKIL"
     gene            9903..10778
                     /gene="wfgD"
     CDS             9903..10778
                     /gene="wfgD"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-Glc:alpha-D-GlcNAc-diphosphoundecaprenol
                     beta-1,3-glucosyltransferase WfgD"
                     /protein_id="AIG56918.2"
                     /translation="MKTSVIITTKNRPQLFREALRSVYNQTVKPDEIIVIDDASSLDY
                     DVFFDTQKIPIIHHKLDTSLGVANARNVGVSLASCDVIFFLDDDDIWNNNHIDSHLRC
                     HYNPDIVMCFSKKELFFCSENNIIRTTKNIEPIESNILSENYIGTPSGVSIKRKYLGG
                     DCIFDVNLPALEDYDLWLQLILKVGNEKIHFVDIPTVKYRISSPKKTNVSHNLRNHEI
                     ARIRIIKKNQGKLTCQELKIIDKYLRFCLLKTAHRNSYYRMIRLLFKYPVFTMKTIVL
                     LIPYSLLNLFGVYSS"
     gene            10789..11607
                     /gene="amsE"
     CDS             10789..11607
                     /gene="amsE"
                     /codon_start=1
                     /transl_table=11
                     /product="amylovoran biosynthesis protein"
                     /protein_id="AIG56917.1"
                     /translation="MMKFSVLMSLYDKEQPEFLNDCLTSLARQSVMPTEIVIVYDGYV
                     NNALKKVVNSYLSILPIKILQLPNNIGLGLALNQGLKYCAYDYVARMDTDDICVDNRF
                     ERQVAHIIQHPELSICGGGISEYDLTLTHFLGNRDVPKCHSEIKKYITMRNPFNHMTV
                     MFKKNDVLSVGGYVHHDLMEDYNLWIRMLSKGFKASNLDGILVHARTGNAMLCRRKGW
                     RYIKSEFYLAKVKIEYNIQSVPYAYFIMVARVVPRLLPTKFLSLFYRITRKTKL"
ORIGIN      
        1 aaaaaataaa tctctacagt gcactggtag ctgttaagcc tggggcggta gcgtgtctga
       61 taatgccctc actggaaggc gaattcaaat gctaaacttc acttatggtg cgtgaatgaa
      121 gaaaaaattg atcataatcg gggctggtgg gtttgctaaa gcagtaatag atagtctgga
      181 tcataatgaa tacgctcttg aagggtttgt tgattcactg aaatccggaa tgcatcaagg
      241 atatcctatc gttggtcatt cattaagtga tattagtttg ccaactgagt actattattt
      301 tattgccatt ggtgatcctg atgatagagc attatggtta agtcaaatac gagatctaaa
      361 gcttgaaacg ataaatgtca ttgataaaac agcaattgta tcaacaagat cttcaatcgg
      421 aacatgtatt tacattggga aaatggctat agtaaattgt gattcacaat tagaggatgg
      481 agtcgtaatt aatactagag ccttgataga acatgggaat catatttcat attgtacgaa
      541 tatttcaact aatgtggtcc ttaatggtga tgtatatgtt ggagagaaaa gttttatagg
      601 tagttgtact gttgtaaatg ggcaaataaa aataggaagc tcgacaattg ttggatctgg
      661 atcagtagta atacgaaaca tccctaacaa tgttgttgtt gcaggaacac caacaagatt
      721 aataagagaa aggtaagcaa atgagtaaaa tatatattgt ggctgaaatt ggatgtaatc
      781 ataatggtga ttttaatctt gcaaaaaaaa tggttgaaga agccaaaaaa gctggtgttg
      841 atgcagtcaa atttcagact tttaaagctg atcaattaat ttcgaaatac gcacctaagg
      901 cagagtatca gataaaagta actggtaatc aggaatccca attagaaatg acaaggaagc
      961 ttgagctgcc ttatgaagag tttataaagc ttgaagaata tgcaaaagag ttaggacttg
     1021 atgtcttttc aacaccattt gattttgaat ctatcgattt tcttgcttca agaaatcaaa
     1081 aaatgtggaa aataccttca ggtgaattgc ttaatctccc gtatcttgaa aaaataagca
     1141 gattgccgat tgagggaaaa gagattgtat tgtctacagg aatggccaca attgaagaaa
     1201 tcaaagcaag tataaatgtt cttactaaaa atggaatggc tcctgaaaag atcacaatat
     1261 tacattgtaa cactgaatat cctacaccat atgaagatgt aaatttaaac tcaattattg
     1321 gtctaaagaa tgaatttaaa ggatataacg ttggcttttc tgaccattca cctggttatt
     1381 atgcagctat tgcatcagtt ccgtatggaa ttacattcat cgaaaaacat tttactctaa
     1441 ataaagattt tgaagggcct gatcataagg catcagttac accagacgag ttgtgcctat
     1501 tgtgtgaggg aatccgtgca gtagaaaaat ctttggggag tcatatcaaa atagtaacga
     1561 aatctgaacg gaaaaataaa attgtagcta gaaagtccat cgttgcaaaa cgtaatatta
     1621 aaaagggtga gatatttact attgataata tcactacaaa acgaccagga aatggaatta
     1681 gcccaatgca ttggtatgat atattgggga aaaaggcaga aaaagatttt gaggaagatc
     1741 aattgattaa gtatagtggt tttaaagaac aagaggttta atttgtgtca agaaagaaaa
     1801 tcgctataat acctgcacga gcgggctcta agggtttgcc agataagaat gttttaatgc
     1861 ttctagatcg tccgttaatt gcgtatacaa ttgaagccgc ggtgagttct aatttatttg
     1921 ataaagttat agtatccacc gattcagaaa gatataaaac gatagcagag tactatggtg
     1981 cagaagtcat gctacgtagt gcagaattat cttctgatac agcaacatct tatatggtaa
     2041 ttgaggatgt tttaaaaaat aatccaggct atgagattat tgcattactt caaccaacgt
     2101 caccgttccg gactgcaaat catattatac aatcaatgga gttatttgaa gaaacagatt
     2161 atgcgagatt tctagtatcg gtatcggaat gttttgcaag tactgattta attaagcctc
     2221 ttgcgcagaa tgggagtcta gaaaattttg acaaagattt tagtaattat cgtcgtcaaa
     2281 attgcagaca atattcaccg aatggggcca tatttttagc atacgtatca gactatttag
     2341 agcaaaaaca tttttttgga aataaaagtg tcgcgtttat tatgaataaa gaggattcta
     2401 tagatataga cgaccattta gacttttgta ctgcgattgc gatacaatct aataaatcaa
     2461 aaataaattc aattcagaat attattcatc agcgtataaa tgaaaaaaaa gaaaagttcg
     2521 aattatgtta tccaataacg ctgattggac attcaatctt tgattactgg gatattgaat
     2581 gtttaaatgg ccaaagagtc aataatttgg gcattgctgg gattaactca gaacaatatt
     2641 atacattaat actagagtct ggattaataa aatcaattgg taatataatt ttattaatga
     2701 ctggaacgaa tgatctcctc aatgaaaatt ggacgccaaa ttatactcta tattggataa
     2761 ataaaactat tcactttatt ttacataata accccaatgc acatatttat tttttagcaa
     2821 ctccaccagt tagagacaga atagatagat ccaatagttc aataaaatac cttaacaaaa
     2881 ttttagaaga gaacattaaa catacccctt gtgttacatg ggtaccattg tgtgatgatt
     2941 tttatgacga atttgattat ttaaaaattg agtttacaac tgatggggtg cattttaata
     3001 aaatcgcata tcagtaccta gagaattata ttaaaggaat tatcaaatga aaaaaatatt
     3061 atatgttaca ggttcacgag ctgaatatgg gattgtgaaa cgattgttag cggctttaaa
     3121 aaatgatcag gatatagagc ttacaattat tgcaacagga atgcattgtg acgagaaata
     3181 tggtttaact tataaacaaa ttgaaaagga tggttttgtt attgaacagc taattaatat
     3241 taatattaat acgctaacga actccgatat aattaactca atgtctgtat gtcaacaaaa
     3301 atttggtcac tattttcaaa ataataaatt cgatgctgtg atgctgcttg gtgatcgcta
     3361 tgaaatattt gcagttgcag ttgctgctgc gattcataat ctacctataa ttcacattca
     3421 tggcggtgaa aaaacattag gcaattatga tgagtttatt aggcattcaa taaccaaaat
     3481 ggcacgttta catttagcct cgacagagca atatcgtaaa agaataattc aaatggggga
     3541 gtgcccgaat agtgttgtta atataggagc attaggagcg gaaaatgcat tacttatgga
     3601 cttacccagt aagagtgaat tagagaaaaa atatggtgcc ttaacacaac cgtattttat
     3661 tgttgttttt caccctgaaa cattaagcca aacatctaca actaagcaaa taagtgagtt
     3721 attaactgct ttgcgagaat taactgaaag ttacaatttt atattcattg gctctaattc
     3781 tgacactggt tctgatgaga taatgaagga gattttaagg ttttgccaac tcactaactc
     3841 taggctcttc aaatcagtaa aaccagaaga atatcttgcc cttaataaat attcgcaggg
     3901 tttaataggt aattcgtcat cgggacttat tgaaatacca tctttattgg taccgacgat
     3961 taatatcggg aatagacaaa aaggaagagt tcgtggcaca acagtaattg attgtgcatg
     4021 ttctgcagaa actattaaaa gtgctattct tttaagtcaa ggagctgatt ttcaacgttc
     4081 attacagttc gcagaaaatc catattataa aaaggcagtc ttagaatccg ctgtaaatgt
     4141 aattaaatca tttgtcttta atgaaaagga gcactacaaa gatttttatg atctcgatta
     4201 gtactattcc tactagatgt gttgggttaa aaatcattaa agtggggata tttcagagta
     4261 taaagagtgt gatattatca ggcataaatt taccagaagt acacttttgt accaaggcaa
     4321 gatcacgtaa cgctgatttt ttgtactaaa tgattcgcat tttatgttta aatattgaga
     4381 tattcctcat tacctaaagc ttttttttat tgcttataca tgatcaaata ctccttacat
     4441 aattaaggag gactaaaaaa tcgatggaac atatttctat tattcccgat tacagacaag
     4501 tctggaaagt ggaacattaa ttgtcagata ttctactgtt gactattcgc gtcgttattt
     4561 ctggtgcaga aggttgggaa gatatagagg attttgggga aacacatcgc gattttttga
     4621 agcaatatgg tgattttgaa aatggtattc ctgttcacga taccattgcc agagtgtatc
     4681 ctgtatcagc cctgcaaaat ttcacgagtg ctttattaac tggatgcgtg attgtcattc
     4741 ttcaggagat aaagacgtca ttgcaattga tggaaaaacg ctccggcact cttatgacaa
     4801 gagtcgccgc aggggagcga ttcatgtcat tagtgcgttc tcaacaatgc acactctggt
     4861 catcggacag atcaagacgg atgagaaatc taatgagatt acaactatcc ctgaactgat
     4921 taacatattg gatattaaag gaaaaattat cacaactgat gcgatgggtt gccagaaaga
     4981 tattgcagag aagatacaaa aacagggagg tgattattta ttcgctgtaa aaggaaacca
     5041 ggggcggcaa aataaagcct ttgaggaaag atttccgctg aaagaattaa ataatacaaa
     5101 gcatgacagt tacgcaatta gtgaaaagag tcacggcaga gaagaaatcc accttcatat
     5161 tgtttgcggt gtccctgatg aacttattga tttcacgttt gaatggaaag gattgaaaaa
     5221 attatgcgtg gcagtctctt ttcggtcaat aatagcagaa aaaaagaaag agcccgaaat
     5281 gacggacaga tattatatca gttctgctga tttaaccgca aagaagttcg ccacagcgat
     5341 cctaaatcac tggctcgtgg agaataagtt gcactgacgt ctgaacgagg taatgaatga
     5401 agacgactgc aaaataagaa gagaaaacgc agaagaacta tttttagtga tacggcatat
     5461 tgctattaat attttaacga gcgataagta ttaaggcggg gttaagacgt aagatgagaa
     5521 aagcagctat ggatagagac ttcatcgctt cagtctttgt ggagaactgg cattcagaat
     5581 cttgctctgt ttaatctcta tgcccgagct gaattataat agtttatatt tgtcaaaagt
     5641 ggatgtgttt atgaataaaa aaaatgcttc acatataggg attttttttt taggtgaaat
     5701 gctttctaag gtattgccat ttctgtacct tttctcacaa gatatctacc tgcttctgat
     5761 tttgggcgtt taggatactt tatgtcttta atgggaatgg ggctaatagc tgttaactgg
     5821 agttttgacg gtgcaattgc gaggtattat tatcgttatg ggaaatttaa tatattaaat
     5881 gtttattata ctgcgattgt tctatcctta gcattaacat ttgtattgtg tctggtggca
     5941 agttttttta ataatagcat atatttatat gcagtagttt gcgcattttt acaaggagta
     6001 ttcaatatat taagtacatt tcaacaatgt caaaaaaatg cttttaaata tattgcaatt
     6061 cagtttacat tttcaataat ttcaactgct ttaacaatag ttttagtgct caagtggcat
     6121 gcagatattg attcgcgaat agttgctata cttgtggggt tatcatgtgc aatttttata
     6181 ggtatgaaat ttttgcatgt ttatagaaaa atacatgtta aagatataat taagaaatta
     6241 aattttacat atctattatc atttgggtta ccattaatat ttcatcagat tagcttaatg
     6301 attaaagggc agttagatag gattatcatc gcaactacgt ttgattatgt tcaattagcg
     6361 cattatactg ctggatatca ggttgcaagt gtaatttcag taggtattat ggcagttaat
     6421 aaggcaattg tccctacata ttatgaagcc tgcaagagta ataaaataaa taaaaataaa
     6481 atatattttt tattctggct tctattacca atttgtcttc tccctagcat aattacatat
     6541 ttcattcctc ctgttttttt cgtggcatta ttaggagaga aatatgagtt cattggcaca
     6601 tatgtaacaa tatttacatt cggttttatg ttgcagatcc catatttatt gttggttaat
     6661 tatttatttt atctaaataa aacacgagtt attgcaaaaa taacaatatt gagtagtata
     6721 gtgcatatag tatcaattta tatacttaag tcttatggga tagtttatat tccatgggcc
     6781 ttggttttat caaatgtctt ctgtattttt cttttgtatt tttatatggg aagaaaagac
     6841 taatgaatgt ttttatttgt tttacaccct tccaactttt tttgtcatta gagattgtaa
     6901 aactcgaaaa tctccccccc agtagatgca ttttttttct ttataattat catgatcgat
     6961 ttaaagttaa aaaaaattat attgaagtaa ctaagggttt tgaacaggtt tattatgagt
     7021 ctcgaccgtt aactctggca tttatattta aacttcgttc tattgcaaaa caaattggcc
     7081 aagttaaaac cgcttacctt gcaagtattg ataacaatat agctcattat ttattgtctt
     7141 ttttaaagcc actttcaatt agaacatttg atgatggtac tgctaatatt actcaaatga
     7201 gtttgtttta cagggatatt aaaaccaatc cactaaaagc tgccttgcac ttattattag
     7261 ggaagaaata taatcgtgaa aaaataaaat cggcctcaga tttacactat acagtgtaca
     7321 gaggggttaa taatatcaca gataaaatag aaatactgga aatgctgcgg gaaaacacta
     7381 gtgatgtgga aaaccggaag gtattaaaaa ttatgctagg ttcaatatat agtgaattgg
     7441 ttgattgttc tgaagatgaa gtttatattc agaattacat ccataggcaa tgtattgatg
     7501 aagagatgtt atatattcct catccacgtg aagatttaag ccgtataaat gttccattcg
     7561 tttcggactg gaggatttca gaacaaatta tcgttgattt tataagttgc gggtatgaag
     7621 tttatttata cgggtttcct agttcaacat tttttaattt aagcagtaat gctcatgtaa
     7681 aatgttgtat ttttatgact gataagttaa gagaagagtt taaagatctt attaaaattg
     7741 caaaatcttt gaatatgtct attacaaata ttcccacagg aagctttaaa tgatacctca
     7801 tcataatctt atttgttcat ttaagctaag attacaaatc tttttcactc tttttacgct
     7861 ttattgtata ttttctttat ggggtatgag tgttgaaaat aaaattttta catatatatc
     7921 gttatttata tcatttctta tagttatttt cgcgttgacc caagggatca gaagtgtttc
     7981 attttttatt ttatttgccg catttttttg tttatcgtca atggctctct ttagtaataa
     8041 aactggtgag cctgttgcta ttcttatcaa tgcatatctt tttgtttttg caatgatgat
     8101 aagcaatatt atttataaac tacgtcttgc atgtcgtagt tcagttatat tattttccac
     8161 tacacatatt tatttcttta ttttttgcat ctataaatat aaaattgatg gattcattaa
     8221 tgaaaatact ttcaatgaac tgttttggca aagtagtcgt aatatagttt ctgctatttt
     8281 acttcttacc actattttct tcttggccgt gaattatatt gaacaaaaaa aaatctatct
     8341 tagtgtgtta tttatcaata tgataagctc aatattattg tatggcagaa gcggtgtggt
     8401 tctgtctttt attgtgttgt ttattgggat atatctaaaa ttctttaaag aagaaaaaaa
     8461 tgtcataaaa attattttgt ttttattctt ttcaatcctc atcttagtgt tgattttata
     8521 tactggattc acagataata tggtcagcgc tatattatct catacaaatc ttcaatcagg
     8581 atttgatacc cctcggattc aaatgtggta tgattacatt gttaattttg attataaaat
     8641 tctgttattg ggtacaaact ttttatttag tccattgatt acatcttttg atgggaatcc
     8701 acataactca tttataatgt ttcaagctag atttggaata tttgcggtat tatttttatt
     8761 gtctattagt tatctatact tgaaactttt tcagaactct ttattgctgt cactgctttt
     8821 aggattgata ttcgttagaa tgttttttga tactttttct ctcttcggat attttgattt
     8881 tatctgggtg tatatgttga tctatctgcc gagaaacaat aaaaagagaa gttttttatg
     8941 agagtgatac ttactttgat tattccagta tataatgttg aagcttatat agaggaatgc
     9001 ttggaaagtt tatttccaca aatagccaat tctaaaaata atgatgtcga agtgattatt
     9061 gtaaatgatg gtacccctga taagtctatg gatatagtca ttcacgctat aaaaaaatat
     9121 agcttggaac gacaggttag agttgttaat cagtgtaatc aaggattaag tgccgctaga
     9181 aatacaggga taagattagc aactggtgat tatataggat ttattgattc tgatgattat
     9241 gttagcgaga attacattcg agaaatcaag tctaatatat tagatttgat tatcagctct
     9301 ggagatatat taaagtttaa ttttcatttc tttaggaata aagtctccag gagtcggcga
     9361 actaatttaa ttaatacggt ccgatactct gggactgagt atattaaaaa tgatatcaaa
     9421 gaaaattctt ggtatgcatg gcgcaggatc tatgcttcgc atttattcga tgatattcta
     9481 tttgacgttg ggaaaaaata tgaagatgtt ttaactgttc ctttgcttac ttttaaatca
     9541 tcaaactgtg ttgctcttaa tagtacttta gttttttata gatcaaatcc ctatgggata
     9601 acaaaaaatt cggagttaga gcatgcaatc gatatcttgt cgagagtaaa tgatcatgtc
     9661 gagataatga aacgacgagg atataataag cagcttattt ctttgcacgt ctataatatt
     9721 gctaaaacat ttgtcaatac tgtagcaact gcttggaaag gtaaattcga catttttttt
     9781 atacactcta atttcactac catctatcca cacctcaatg ttttccgaac tgtttatgtc
     9841 tatgtttact attttaaagc agttttgaag tatttgcttt cacaagttaa aagaaaaata
     9901 ttatgaagac cagtgtcatt attactacga aaaataggcc tcagttgttt agagaagcac
     9961 tacgttctgt ttataatcaa acagtcaagc ctgatgagat aattgttatc gatgacgcat
    10021 cttctctcga ttatgacgtt tttttcgata ctcaaaaaat accgataatt catcataaat
    10081 tagatactag tcttggagtg gcaaatgcaa gaaatgttgg tgtatcttta gcttcctgtg
    10141 atgttatttt ttttcttgat gatgatgata tctggaataa taatcacata gatagtcact
    10201 tgcgatgtca ttacaatcca gatattgtaa tgtgtttttc aaaaaaagaa ttgttttttt
    10261 gttcagaaaa taatattata agaacaacca aaaatattga gccaatagaa tctaacatcc
    10321 tttctgaaaa ctatattggt actcccagtg gtgtttctat caagcgaaaa tatcttggcg
    10381 gtgattgcat atttgatgtt aatttacctg cattagagga ttacgacctt tggttacaat
    10441 tgattttaaa agttggcaat gaaaaaattc atttcgttga tattccgaca gtaaaatata
    10501 gaatatcatc accaaagaaa acaaatgtta gtcataatct acgtaaccac gaaattgcgc
    10561 gaatcaggat aataaaaaag aatcaaggga agcttacttg tcaggaatta aaaataatag
    10621 ataaatatct tcgattttgt ttactaaaaa cagctcatag aaattcctat tatagaatga
    10681 ttcgacttct ttttaagtat ccggtattta caatgaaaac tatagttttg ttaattccct
    10741 attcgttgtt aaatttattc ggtgtttatt ccagttgagc aatgttgaat gatgaaattt
    10801 tctgtattaa tgtctcttta tgataaagaa caacctgagt ttttgaatga ttgtttaact
    10861 agtttagcta gacaaagtgt tatgcctacc gaaatagtca ttgtttacga tggctacgtc
    10921 aataatgcat taaagaaagt tgtaaatagt tatttatcca tcctacctat caaaatattg
    10981 caacttccta ataatattgg tttaggcttg gccctgaatc agggccttaa atattgcgca
    11041 tatgattatg ttgcccggat ggatactgat gatatttgtg tggataatcg ctttgaacgt
    11101 caagtcgctc atattatcca acacccggag ctatctattt gtgggggggg gatttctgaa
    11161 tatgatttaa ccttaactca ttttttggga aatcgagatg tccctaaatg tcattctgaa
    11221 ataaaaaaat acataactat gcgaaatcca ttcaatcata tgactgtaat gtttaaaaaa
    11281 aatgatgtct tgagtgttgg tggatacgtt catcacgatt tgatggagga ttataacctt
    11341 tggatacgaa tgttgagtaa aggttttaaa gcatctaatt tagatggtat cttggtgcac
    11401 gctcgtacgg gcaatgcaat gctgtgtcgt cgtaaagggt ggcgatatat taagagtgaa
    11461 ttttatttag caaaagtgaa aattgaatat aatatccaaa gtgttcctta tgcttacttt
    11521 attatggttg ctagggtggt tcctcgatta ttaccaacaa aattcctctc tctattttat
    11581 cgtatcacca gaaaaaccaa gttataatag ttatttattt tagttcaaaa taatagattt
    11641 ttcctgagtt aatatatcaa ctgtatttca agccgcgcac cacaagcggt gacacacctg
    11701 acaggagtaa acaatgtcaa agcaacagat cggcgtcgtc ggtatggcag tgatggggcg
    11761 caaccttgcg ctcaacatcg aaagccgtgg ttataccgtc tctattttca accgttcccg
    11821 tgaaaaaacg gaagaagtga ttgccgaaaa tccaggcaaa aaactggttc cttactatac
    11881 ggtgaaagag tttgt
//

</pre>