<pre>LOCUS       KJ778796               13036 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain 1792-54 serotype O169:K-:H8 O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ778796
VERSION     KJ778796.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13036)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13036)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Swimley,M.,
            Baranzoni,G.M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="1792-54"
                     /serotype="O169:K-:H8"
                     /db_xref="taxon:562"
     misc_feature    1..13036
                     /note="O-antigen gene cluster"
     gene            85..1053
                     /gene="epsJ"
     CDS             85..1053
                     /gene="epsJ"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="AIG62718.1"
                     /translation="MDDSVSVVIPYYNDSPRIEKCLDSICSQTRQAIEVIIVDDCSKD
                     SALLLKIIDKFKNKINIRYLRNDENKNGAYSRNVGMREARGGIVAFLDADDYWATDHL
                     EESVNALLLNGVEFVFSNVIEVDCCGCESRRKVTNPQYLDNKYDIILLSPPQTNSFLF
                     RKKIFDTKEIFFDENLRRHQDYQFLTLILEKNISYQYIDKYTSYYVQSHRPHTERMDY
                     KSVFLFWEKYNSYVSGRLLKKFITGLVLEVVLVYGKNKTKSFIDSHELVKINASKLFL
                     YLVLKTNFGVNANRVIYPFFYYILFSPKSILKKISQRINRKLVHNA"
     gene            1046..2155
                     /gene="pglH"
     CDS             1046..2155
                     /gene="pglH"
                     /codon_start=1
                     /transl_table=11
                     /product="galNAc-alpha-(1->Feature
                     gb|4)-GalNAc-alpha-(1->Feature
                     gb|3)-diNAcBac-PP-undecaprenol
                     alpha-1,4-N-acetyl-D-galactosaminyltransferase"
                     /protein_id="AIG62719.1"
                     /translation="MRNKISIVTTNITNNGGTERVIANVANSLSEIYDIRIYSLSTTE
                     GECFYPLNERIHIKHMGMMCYEHEKSVLKKTIKKLINIFYSINEFKNIKSNYIIGVNK
                     NINILLVLSFLLNYKRKRDYKLIGWEHFAHNAPMSLFTRILRDLLYKYLDDLIVLTKF
                     DEEYYKAKKIKTTVIENAYTAMDVDNLKTVSKGNIILSIGRHTSQKRFDKLLYIWKEI
                     VKKNDRLKLRIVGDGPLLDENKKLAQKLNLSDSVVFAPPTKKINEEYQNASLFLMTSD
                     YEAFPMVLLEALKNGLPCIAFDCDTGPRDIIVNGVDGFVIPLNDSLKFVEKTTDVMNN
                     ENLRYQLSSNAKANVERFNEEHISKKWLIKLKQLQ"
     gene            2182..3234
                     /gene="wzy"
     CDS             2182..3234
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AIG62720.1"
                     /translation="MIIITSFMVLLLLFTDNLKLKNFRIILFYFFSLCFSVLVYFRPE
                     YMLNTDYKEYYNYFKWINFDNVYKLSDAIGFEYGFGYIVTLIKYFFESERACFSGIAF
                     LSVFISYKAILRLNPKTTAFSFSLFLLSFCVYNFLGQIRQGISLSLGLLAISYLLEDK
                     RKQFLLTVIIASSIHVTALILILAPFVKYFRIKYIFYSLLISFTFVFIDVIKPLIITL
                     VQFIPFGSFITGKIIAYGNSEFSTKVGFSFIQVYYVLLSTVLYILEQKYEYKNNHILI
                     LCKIFMVGVILNFTFNSFSVLLRLTYYYLAFDCILLGYLLNMAKNGYTKILIYCPTLM
                     LFILRFYMQWSEHING"
     CDS             3227..4225
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-D-galactose:(glucosyl)lipopolysaccharide-1,
                     6-D-galactosyltransferase"
                     /protein_id="AIG62721.1"
                     /translation="MVEHCSKNKKILVDCRMINASGIGVYTREIIKELKKYRYLQIEL
                     LLQEEQHLPVDVEDIGFKIHYINFSRYSIKNICGIKHLLNGFDVYFMPSLSIPPLLKK
                     GCLTITTVHDLCPVALRKLFGFKKAVAYWALLLIQLVCSNKIISISRFTAKEINKYYL
                     GLFKSKLYVIGNGIRNIFNLKLNSETEISWEEYGICVGNIKPHKNVVPLIDYLKTNKI
                     NKKIYFIGEINGFSTKVNKSLLNDLPENIVFTGKVSDQELHNYYRNASFFIFPSLYEG
                     FGLPLLEAMNYRLPIFASNIDVFKEVAGETINYFDPYTFDDLADRINKLKIFQEIY"
     gene            4294..5412
                     /gene="pimA"
     CDS             4294..5412
                     /gene="pimA"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose-dependent
                     alpha-(1-2)-phosphatidylinositol mannosyltransferase"
                     /protein_id="AIG62722.1"
                     /translation="MKKILLVNKYYHPDIGGVETVVQQHAQLLKDEYSVTVLCISKEF
                     SWYTKNEFINGVKVIRCSSLGTYFSMPLSITFFLHYFINYLKSDVIINHVPFPLMDLA
                     FFIIDRLVKRENILFWHSDIVKQKALKKILRPFIINTINRSTQILTTSPNLKANSEDI
                     GEYEKKTDVLPLFINSNVINGYIKNLNAEKKYDFIFFGRLCYYKGVDVLLDAVKILKN
                     KGAEPEIFMAGDGDLVTYIENEIIENKIKNITFIKRFLTEEEKYHYLSISKCFLFPSV
                     AASEAFGITQLEAMSLGLPVINTNLNTGVPFVSLNGVTGITVTAGNPDDLANAMLSLL
                     RDNEKYESFKTNCKVRVSELFEENVVKNKLFKVIEELN"
     gene            5493..6929
                     /gene="manC"
     CDS             5493..6929
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase 1"
                     /protein_id="AIG62723.1"
                     /translation="MSNNIICPVIMAGGSGSRLWPLSRLFYPKQFLKLVDDSSMLQET
                     ISRLGDLPCSLPVVICNEQHRFIVAEQLRMINKLTNNIILEPVGRNTAPAIALAAMIS
                     ESFSSSEDPLLLVLAADHDIKNHKSFRDAVRKAIPFAEQGKLVTFGIVPSMAETGYGY
                     IKRGHEISLENDEVAYTVSKFYEKPNISDAQKFLASGEFYWNSGMFLFRASRYLSELN
                     KFRPDIYLACASSTKNINTDLDFIRVDENSFSTCPDESIDYAVMEQTADAVVVPMNAG
                     WSDVGSWSSLWEISKKNEQGNVNHGDVINHNTENSYVYAESGLVTTIGVKDLVVVQTK
                     DAVLVADRNSVQDVKKVVEQIKAHGRSEHQMHREVYRPWGKYDSIDAGERYQVKRITV
                     KPGEGLSVQMHYHRSEHWVVVAGTAKVTIDGVIKLLGENESLYIPQGAKHCLENPGKI
                     PLELIEVRSGSYLEEDDVVRFADRYGRV"
     gene            7043..8416
                     /gene="algC"
     CDS             7043..8416
                     /gene="algC"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="AIG62724.1"
                     /translation="MKNLSCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALDAPVEFIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESKSDAKLMQLKTQEI
                     LTIMNNI"
     CDS             9135..9689
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DDE domain protein"
                     /protein_id="AIG62725.1"
                     /translation="MRDCHSSDDKDVIAIDGKTLRHSYEHDSYAISEKSHGREEIRLH
                     IVCDVPDELIDFTFEWKELKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFA
                     TAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGL
                     RRKMRKAAMDRNYLASVLAGSGLS"
     CDS             complement(9660..10097)
                     /inference="ab initio prediction:Prodigal:2.60"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="AIG62726.1"
                     /translation="MNKEIQQRLQWVKLYETSGDAGFVCRRCGISRPTLRKWWRRCLA
                     QGIAGLESHNRRPKRSPSTKTGTCEVALILELRSQRNLGARPIQSELKRLHSISLAIA
                     TINKVLCQNQVKPVVKFQRKADFIRYERPVPRARLRKPAPRKD"
     gene            10357..11640
                     /gene="wzx"
     CDS             10357..11640
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AIG62727.1"
                     /translation="MKEWDLKRNIFTNSLWMILEKAIAILGLIFVTSYVAKYVGTEIF
                     GEIAYATSLFQIVQVVSQLGSDVLIFKRISKNTKSGIKLIQSTIFIRIMIYCILSLLI
                     LFYLLKEHSEFSHVYMVAAMLSCFFSAMDVYSIYYDVNLLSKYNTIVNALGLTITLLI
                     RWIIAFYELKPEFLCIPIILTGLIPFVIRFVLFRINEDSSYIPFKHMKKYIMYMIKAG
                     STIVISSVSVAIYTRVSVIVLGFFLDKESVGVYSVAASLGMAWSFIPNSFITSSLPSI
                     FSEKDNSKAIVKAANLNLLVFFVCIPFIVGGYLFGFYFIKLLYGDSYIAAYYPMVLLC
                     IATMFAALGTVASRFITHYSGYSFLSKKTILVLFFSLILSCFFVKYHGVTGAAISIVI
                     TEVLSLTLFNYLFNKGIVFKLHLKTVGKALERING"
     CDS             11633..12811
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="AIG62728.1"
                     /translation="MVKKLVGLAKNNHPEKRCIADTEIYNVRNFKKNNYYYYYGYLLR
                     LLKKDCLGTFKFRPILKKILHDVDCFMFFNDIGYLDDNDWISFFETTIPRLPSIKDIH
                     RDHNDKINYPRLNEVKTDLESLVHENCKRLIAISNSAFEIQSTLLKSYPEYKDIILNK
                     TEVIHPPQRLLFKDKLKSTKFKFIFVGNDFYRKGGGEVVLAFQKLIKEYSFLNKNVSL
                     ILIGDLNRKYNYVHGEFQFKISYFKQIEDIIYSSNNIRLYTNISNMSVLKLMSESSVG
                     ILPTWGDTYGYSVLEMQSFGLPVITSNVRALPEINHNDLIFNVDTNILGEIVIDSFEQ
                     RDAISEKIIDGVKKKMEYILLGDNKLHMFSEWSIDNIRTNHDPDIFYKKILNIIDEKR
                     "
ORIGIN      
        1 gtagaataaa aagtgcactg gtagctttaa gccaggggcg gtagcgtgtc aaatatatat
       61 tttattattg agtggcttac atatatggat gattcggttt ctgtcgttat accttattac
      121 aatgattccc ctcgtattga aaagtgtttg gatagtatat gttcacaaac cagacaagct
      181 attgaagtta taatagttga tgattgttca aaagatagtg cattactact aaaaatcatt
      241 gataaattta aaaataaaat aaatatacga tatttgagaa atgatgaaaa taaaaatggg
      301 gcatattcca gaaacgtagg gatgagagaa gcaaggggag gaattgttgc ttttctcgat
      361 gctgacgact attgggccac agatcatctc gaggaaagtg taaatgcttt gttgttaaat
      421 ggggtggaat ttgttttttc aaatgtaata gaggttgatt gttgtggttg tgagagtcga
      481 agaaaagtta ctaatcccca atatttggat aataaatacg atataatact gctttctcct
      541 cctcaaacta attcattcct atttaggaaa aaaatatttg acactaaaga gatttttttt
      601 gatgaaaact tacgtagaca tcaagattat caatttctta ctttaatatt agaaaaaaat
      661 atttcatatc aatatattga taaatatact tcatattatg tgcaatcaca tcgtcctcat
      721 actgaaagaa tggattataa atctgttttt ttattttggg aaaaatataa ttcttatgtt
      781 tctgggaggt tactaaaaaa atttattacc ggtttagttc ttgaggtggt tcttgtgtat
      841 gggaaaaata aaacaaagag ttttattgat tcgcatgaac ttgtcaagat aaacgcatcg
      901 aaactatttc tttatcttgt gttaaaaaca aattttggtg ttaatgctaa tagagtgatt
      961 tatccttttt tttattatat tttattcagc cccaaaagca tattaaaaaa gatatcacaa
     1021 aggattaata gaaagttggt acataatgcg taataagata tctatagtta ctacaaatat
     1081 tactaataat ggtggtactg agcgagtaat agcaaatgtt gctaattctc tttcagaaat
     1141 ttatgatatt cgtatttata gcctatctac tactgaaggc gaatgttttt atccactaaa
     1201 cgaacgtata catataaaac atatggggat gatgtgttat gagcatgaaa aatcagtttt
     1261 aaaaaaaaca atcaaaaagt taataaatat tttctattca ataaatgaat ttaaaaacat
     1321 aaagtcaaat tatataattg gagtcaacaa gaacataaat atacttctag ttctatcgtt
     1381 tttgttaaat tacaaaagaa agagagatta taaattgatc gggtgggaac attttgcaca
     1441 taatgctcct atgtcactat ttaccagaat tttacgtgac ctattatata aatacctaga
     1501 tgatttaatt gttctaacta aatttgatga ggagtattat aaagctaaga aaataaaaac
     1561 aactgtgatt gaaaatgcgt atacagcaat ggatgtagat aatctcaaga ctgttagtaa
     1621 aggaaatatt attctatcaa ttggaagaca tacatctcaa aagcggtttg ataagttgtt
     1681 atatatctgg aaagaaatag ttaaaaaaaa cgatagatta aaactgcgga ttgttgggga
     1741 tggtcctcta ttggatgaga acaaaaaatt agcccaaaaa ttgaatctgt ccgactcagt
     1801 agtatttgct ccgccaacta aaaaaattaa tgaggaatat caaaatgcat ctttattttt
     1861 aatgacatct gactatgaag catttccaat ggttttgtta gaagctttga aaaatggtct
     1921 tccttgcatt gcatttgact gtgatactgg acctagagat attattgtta atggagttga
     1981 tggatttgtt ataccattaa atgatagttt gaaatttgta gaaaaaacaa cagatgtcat
     2041 gaataatgaa aacttaagat accaactaag ctcgaatgca aaggctaatg tcgaaaggtt
     2101 taatgaagaa catatttcaa aaaaatggtt gattaagtta aaacaattgc aataatatcg
     2161 acgttgaaat caaggtataa gatgattata ataacatcat tcatggttct gttgctttta
     2221 tttacagata atttaaaatt aaaaaacttc agaataatat tattttattt tttttcgctt
     2281 tgtttcagtg tgttagttta ttttagacct gaatacatgt taaatactga ttacaaagag
     2341 tattataact attttaaatg gattaatttt gataatgttt ataagttatc ggacgcaatt
     2401 ggttttgaat atggcttcgg ctatatagtt acgttaataa aatatttttt tgaatctgaa
     2461 cgtgcttgtt tttcaggtat tgctttttta tcggtgttta ttagttataa agctatatta
     2521 agattaaacc caaaaacgac cgcattttct ttttcattat tcttattatc attttgtgtt
     2581 tataattttt taggccaaat aagacaaggc atttcacttt cgttaggctt actggctata
     2641 tcttatttgt tggaggataa gcgaaaacag tttttattaa ccgttatcat agcttcaagc
     2701 attcatgtga cggccctaat actaatacta gctccattcg ttaaatattt taggataaag
     2761 tatatttttt attccttact aatatcgttt acttttgttt ttatagatgt aataaagcca
     2821 ctaattataa ctttagtgca gtttattccg tttggtagtt ttataaccgg aaaaataata
     2881 gcttatggaa atagcgaatt ttcaaccaaa gtgggttttt cttttattca agtgtattat
     2941 gttttacttt ctacagtttt atatattctg gagcaaaaat atgaatataa aaataatcat
     3001 atcctcatat tatgtaagat ttttatggtc ggagtgattc ttaattttac atttaattca
     3061 ttttctgttt tacttagact tacttattac tatttagcat ttgattgcat tttgttaggc
     3121 tatctcttga atatggcaaa aaatggatat acgaaaatat taatatattg cccaacacta
     3181 atgctattta tattaaggtt ttatatgcaa tggagcgaac atatcaatgg ttgaacattg
     3241 tagtaaaaat aaaaaaatac ttgttgattg tagaatgatt aatgcctctg gaataggtgt
     3301 ttatactcgt gaaattatca aagagttaaa aaaatataga tacttgcaga tcgaattatt
     3361 gcttcaagaa gaacaacatt taccagttga tgttgaagat ataggattta aaatccacta
     3421 tataaatttt tctcgctata gtattaaaaa catctgtggt attaagcatt tgctgaatgg
     3481 ctttgatgtc tattttatgc catcattatc tataccacct ctattaaaaa aaggctgttt
     3541 gacaatcaca acagtacatg atttgtgtcc tgtggcatta cggaaattat ttggattcaa
     3601 aaaagctgta gcttattggg ctttgctatt aatacaactt gtttgttcta ataagataat
     3661 ctccatttca agattcactg ctaaagaaat aaataagtat tacttaggtt tatttaaaag
     3721 taaattatat gtcattggca atggtataag aaatattttt aatttgaaat taaatagtga
     3781 aacggaaatt tcctgggaag aatatggtat ttgcgtaggc aatattaaac ctcataaaaa
     3841 tgttgtacca ctaattgatt atcttaagac aaacaaaatt aacaaaaaaa tatattttat
     3901 aggtgaaata aatgggtttt caacgaaagt aaataaaagt ctgcttaacg atttgcctga
     3961 aaatatagtt tttacaggaa aagtaagtga tcaagaatta cataattatt accgtaacgc
     4021 cagttttttt atattcccct cattatacga aggctttggt ttgcctttgc tggaggctat
     4081 gaattatagg ttgcctattt ttgcatcaaa tattgatgta tttaaggaag ttgctggtga
     4141 aacaattaat tattttgatc catatacatt tgatgatctt gcagatagaa ttaacaaatt
     4201 aaaaatattt caagaaatct attgaatctt atgcaagagt tttgcagtta tatagttggg
     4261 ataataatgt tgcaagtcta ttaaggatta tcaatgaaga aaatattgct cgtaaataaa
     4321 tactatcatc cagacattgg tggcgtagaa actgttgtac aacagcatgc tcaattattg
     4381 aaggatgaat atagtgtaac agtgttgtgt attagcaaag aattctcatg gtatacaaaa
     4441 aatgaattca ttaatggggt taaagtaata cgctgttctt cattgggaac ttatttttct
     4501 atgccgctat caattacttt ttttttgcat tacttcatta attatttaaa atcagacgtg
     4561 attattaatc acgttccgtt tccattaatg gatttagcat tttttatcat tgatagatta
     4621 gtcaaaagag aaaatatatt attttggcac agtgatatag taaagcaaaa agcattaaaa
     4681 aaaatattac gtccgttcat tataaatact attaatcgaa gcactcagat tttaactaca
     4741 tctccaaatc tcaaagctaa ttcagaagac atcggtgaat atgagaaaaa aacagatgtg
     4801 ttgccattat ttattaattc aaatgttata aatggttata ttaaaaattt aaacgcagaa
     4861 aaaaaatatg actttatatt ctttggaaga ttatgttatt ataaaggagt tgatgttctt
     4921 ttagatgcag taaagatttt gaaaaataaa ggagctgaac ctgaaatttt tatggcaggg
     4981 gatggggatt tagttactta tatcgaaaat gaaattattg aaaataaaat taaaaatata
     5041 acttttataa aacggttttt aacagaggag gaaaaatatc attatctttc aatttcaaaa
     5101 tgttttcttt tcccatccgt tgctgcatcc gaagcatttg gtatcactca gcttgaggca
     5161 atgtctcttg ggcttcctgt aataaacaca aaccttaata ctggcgttcc ttttgtcagt
     5221 ttaaatggag ttacggggat aacagtaact gctgggaatc ctgatgattt agctaatgca
     5281 atgctgtctt tgttgcgcga taatgaaaaa tatgaatcgt tcaagacaaa ttgtaaagtt
     5341 agggtttcag agttgtttga ggaaaatgtt gtgaaaaata aactattcaa agtcattgaa
     5401 gaattaaact gagaagttga attttcactc atactatatt cattgattat tcgtatttat
     5461 aaacttaact ttcgtattta taaacttaac ttatgtcaaa taatattatc tgtcctgtga
     5521 ttatggctgg tggttctggt agccgtctat ggcctctttc tcgtcttttt tatccaaagc
     5581 aatttctaaa attagtagat gattcaagta tgctacaaga aacaattagt cgccttggag
     5641 atcttccttg tagcttacct gttgttatat gtaatgaaca gcatagattt attgttgctg
     5701 aacagcttcg aatgattaat aagttaacga ataacattat attagaacct gtagggcgta
     5761 atacagcccc agcaatagct ttagcagcaa tgatctctga gtctttttct tcttcagaag
     5821 atcccttact tttagtatta gctgctgatc atgatattaa aaatcataaa tcatttcgtg
     5881 atgctgtaag gaaagctatt ccttttgcag aacaaggtaa attagttact tttggtattg
     5941 ttccaagtat ggctgaaaca ggatatggct atattaagcg tgggcatgaa atttctttgg
     6001 aaaatgatga agtcgcgtat actgtttcga agttttatga aaaacctaat ataagtgatg
     6061 cccaaaaatt cctcgcttct ggagaatttt attggaatag tggtatgttt ttatttagag
     6121 cttctcgtta tttgagtgag ttaaataaat ttagaccaga tatatactta gcatgcgcat
     6181 cttctactaa aaatataaat accgatcttg attttattcg tgttgatgaa aattcatttt
     6241 ctacctgccc agatgaatcc atagactatg cagtcatgga acaaactgct gatgcagtgg
     6301 ttgttcctat gaatgcaggt tggagtgatg ttggatcatg gtcttcccta tgggagatta
     6361 gtaaaaaaaa tgaacaaggg aatgttaatc atggtgatgt tattaatcac aatactgaga
     6421 atagttatgt ctatgccgag tctggtttgg tgactaccat tggggtaaaa gacttggttg
     6481 ttgtacaaac caaagatgcc gttcttgttg cagatcgcaa ttcggtgcag gatgtgaaaa
     6541 aagtagtgga gcagattaaa gcccatggtc gttctgaaca tcagatgcat agagaagttt
     6601 atcgtccatg gggtaaatat gactctatcg acgcaggcga gcgctaccag gttaaacgca
     6661 taaccgtgaa accgggcgag gggttgtcag tacagatgca ttaccaccgt tcggaacact
     6721 gggtagtggt agcgggaacg gcaaaagtca ctattgatgg ggttatcaaa cttcttgggg
     6781 aaaacgagtc cctctatatt ccgcaagggg cgaagcattg cttggaaaac ccggggaaaa
     6841 taccgcttga gttaatagaa gttcgctccg gttcttatct cgaagaggat gatgtggtgc
     6901 gttttgcaga tcgctatgga agagtctagt tttgtacaac tttcattttt cctaataatt
     6961 ctcgatctga aataattctt ccctgttcat gtaaaatatt tgaataccac tctaatttga
     7021 gtgttgaaaa tgagtgttga aaatgaaaaa tttatcctgc tttaaagcct atgatattcg
     7081 cggaaaatta ggcgaagaac tgaatgaaga tattgcctgg cgtattggtc gcgcctatgg
     7141 cgaatttctt aaaccgaaaa ccattgtgtt aggcggtgat gtccgcctca ccagcgaaac
     7201 cttaaaactg gcgctggcga aaggtttaca ggatgcgggc gtcgatgtgc tggatattgg
     7261 catgtccggc accgaagaga tctatttcgc cacgttccat ctcggcgtgg atggcggcat
     7321 cgaagttacc gccagccata acccgatgga ttacaacggc atgaagctgg tgcgcgaagg
     7381 ggctcgcccg atcagcggtg ataccggact gcgcgatgtc cagcgcctgg ctgaagccaa
     7441 cgactttcct cccgttgatg aaaccaaacg cggtcgctat cagcaaatca acctgcgtga
     7501 cgcttacgtt gatcacctgt tcggttatat caatgtcaaa aacctcacgc cgctcaagct
     7561 ggtgatcaac tccgggaacg gcgcagcggg tccggtggtg gacgccatcg aagcccgctt
     7621 taaagccctc gacgcacctg tggaattcat caaagtgcac aacacgccgg acggcaattt
     7681 ccccaacggt attcctaacc cgctactgcc ggaatgccgc gacgacaccc gcaatgcggt
     7741 catcaaacac ggcgcggata tgggcattgc ctttgacggt gattttgacc gctgcttcct
     7801 gtttgacgaa aaagggcagt ttattgaggg ctactacatt gtcggtttgc tggcagaagc
     7861 gttcctcgaa aaaaatcccg gcgcgaagat catccacgat ccacgtctct cctggaacac
     7921 cgttgatgtt gtgactgccg cgggcggcac gccggtgatg tcgaaaaccg gacacgcctt
     7981 tattaaagaa cgtatgcgca aggaggacgc catctacggt ggcgaaatga gcgcccacca
     8041 ttacttccgt gatttcgctt actgcgacag cggcatgatc ccgtggctgc tggtcgccga
     8101 actggtgtgc ctgaaaggaa aaacgctggg cgaactggtg cgcgaccgga tggcagcatt
     8161 tccggcaagc ggggagatca acagcaaact ggcgcaaccc gttgaggcga ttaaccgcgt
     8221 ggaacagcat tttagccgcg aggcgctggc ggtggatcgc accgatggca tcagcatgac
     8281 ctttgccgac tggcgcttta acctgcgctc ctctaacacc gaaccggtgg tacgcctgaa
     8341 tgtagaatca aaaagcgatg caaaattgat gcaattaaaa acacaagaga ttctgactat
     8401 aatgaataat atttgatgaa gtaaaatgat aatataaata agtctgtgtt gtcattagga
     8461 aaatacttta tctattatgc aaaatttgtt tataaatgga ttaaagatat tctattttta
     8521 tgtcttaatt tataccccta aggtgaagag aaataatgca aaggatgtta aaaaatataa
     8581 acatacaggg aaagaacgtt gtatgcctta gatataaaga tacgaaactg aacgtattgc
     8641 atgtaataag catcagtttg tgagatggtg gcaggtccac cgggatctgc tttcagaagt
     8701 cgctgaaacg tctaagtaaa gattacgtga tgttgctttt ttgtactaag caattcgcat
     8761 tttatgttca ataattgaga tattcctcat tacctgaagc tctttttcat tgcttatacc
     8821 tgccaaatac tccttacata attaaggaga acaaaatgga acttaaaaaa ttgatagcac
     8881 atatctctat tatccccgat tacaggcaag cctggaaagt ggaacataaa ttgccagata
     8941 ttctatctgt taactatttg cgccgttatt tctggtgcat aatgctggga agatatagag
     9001 gatttgggga aacacatctc gattttttga agtgatatgg tgattttgaa aatggtattc
     9061 ctgttcacga tatcattgcc agagttgtat cctgtatcag tcctgcaaaa tttcatgagt
     9121 gctttattaa ctggatgcgt gactgccatt cctcagatga taaagatgtc attgcaattg
     9181 atggaaaaac gcttcggcac tcttatgagc atgacagtta cgcaatcagt gaaaagagtc
     9241 acggcagaga agagatccgt cttcatattg tttgcgatgt ccctgatgaa cttattgatt
     9301 tcacgtttga atggaaagag ctgaagaaat tatgcgtggc agtctccttt cggtccataa
     9361 tagcagaaca aaagaaagaa ccagaaatga cggtcagata ttatatcagt tctgctgatt
     9421 taaccgcaga gaagttcgcc acagcaatcc gaaaccactg gcacgtggag aataagctgc
     9481 actggcgtct ggacgtggta atgaatgaag acgactgcaa gataagaaga ggaaatgcag
     9541 cagaattatt ttcagggata cggcacattg ctattaatat tttgacgaat gataaggtat
     9601 tcaaggcagg gttaagacgt aagatgcgaa aagcagccat ggacagaaac tatctggcgt
     9661 cagtccttgc ggggagcggg ctttcgtaat cttgccctgg gaacagggcg ttcgtagcga
     9721 atgaaatcag ctttacgctg aaatttaaca acaggtttaa cctgattctg gcaaaggact
     9781 ttgttgattg ttgctattgc cagtgagatt gagtgaagtc gttttaactc gctttgaata
     9841 ggtcttgccc ccagatttcg ctgagaacgt aactccagaa tcagagcgac ttcgcaagtg
     9901 ccggttttcg ttgatggtga acgcttgggg cgacggttgt ggctttccag accagcgatg
     9961 ccctgagcca gacatctgcg ccaccattta cgcaatgtgg gtcgagaaat gccacagcga
    10021 cggcatacaa atcccgcatc accggacgtt tcatagagtt ttacccactg tagccgctgc
    10081 tgaatttcct tgttcatagt gaggtattat agtgaaacga tgtctatgaa tcacacagct
    10141 gaattcgatg atatcggctt taaggaatta tatacatagc aaatatgaaa aattatacgg
    10201 aaagattttt tagtgataaa aaacttgttt ttcatttatt gataggaaat aagaataaat
    10261 cacaaaggta gatattgcct tgcggtgtga tatttgtgaa atatctcctt tgattacgca
    10321 ttatataaat gataatgtgt tgcttcttct ttttatatga aggaatggga tttgaaaaga
    10381 aatatattta ctaactctct gtggatgatt ttagaaaaag ctattgccat attagggctt
    10441 atttttgtta catcctatgt tgcaaagtat gttggtacag agatttttgg tgaaattgct
    10501 tatgcaacat cactattcca aatagttcag gttgtttctc aactaggaag tgatgtgttg
    10561 atttttaaaa gaattagcaa aaatacaaaa tcaggtataa agcttattca atcaactata
    10621 tttattcgta taatgattta ttgtatctta tctttattga ttttattcta tttgcttaag
    10681 gaacatagtg aattttcgca tgtgtatatg gttgcagcta tgttatcttg ttttttttca
    10741 gccatggatg tttattctat atattatgat gttaacttat tatccaaata caatacaata
    10801 gttaatgccc ttgggttaac aataacgtta ttaatacgtt ggataattgc cttttatgag
    10861 ttaaagcctg aatttttatg cattcctatt atattgactg gattaattcc ctttgtaata
    10921 aggtttgttc tatttagaat taatgaagac tcttcataca taccatttaa acatatgaag
    10981 aagtatatta tgtatatgat taaggccggt tcaacaatcg taatatcatc agtgtcagtt
    11041 gccatatata ctcgtgtttc agttattgtc cttggtttct tcttagataa agaaagtgtt
    11101 ggagtatatt ctgtcgctgc gtcgcttggt atggcatggt cttttattcc aaattcattt
    11161 atcacttctt ccttaccaag tattttttcg gaaaaagata attcgaaagc aattgttaaa
    11221 gcggcaaatt taaatttgct tgtttttttt gtttgtatcc cctttattgt tggaggttat
    11281 ctttttggtt tttattttat aaaactatta tacggagata gttatattgc ggcatattat
    11341 ccaatggttc ttttatgtat cgcaacaatg tttgctgcat taggaactgt agcttcaaga
    11401 ttcattacac attattccgg ttattcgttt ttgtccaaaa aaacaattct tgttttgttt
    11461 ttcagtctaa tacttagttg tttttttgtc aaatatcatg gtgtcacagg tgcagctata
    11521 tctatagtaa taactgaggt gctatctctg acacttttta attatttatt taataaaggg
    11581 attgtattta aattacatct taaaacagta ggtaaagcat tggagaggat aaatggttaa
    11641 aaagttagtt ggacttgcaa aaaataatca ccccgaaaag agatgtattg cagatactga
    11701 aatttataat gtgaggaatt tcaaaaaaaa taattactat tattattatg gttatttgtt
    11761 gaggttgttg aaaaaagact gcttgggtac atttaaattt cgtccaatct taaaaaaaat
    11821 tttgcatgac gttgattgtt ttatgttctt taatgatata ggttacttag atgataatga
    11881 ttggattagt tttttcgaaa ctacaatccc taggttgcct tcaataaaag acattcatag
    11941 agatcataac gataaaataa attaccctcg attaaatgag gtcaaaaccg atttagaaag
    12001 cttagttcac gagaattgta aaagactaat tgccatatca aacagtgcat ttgaaattca
    12061 gtctacatta ttaaaatcct atccagaata taaagatatt attttaaata aaacagaagt
    12121 tattcatcca ccacagagac tattgtttaa agataaatta aaaagcacaa aatttaaatt
    12181 tatttttgtt gggaatgatt tttataggaa gggaggtggt gaagttgttt tggcatttca
    12241 aaagctaata aaggaatata gctttctaaa taaaaatgtt agcttgattt taataggtga
    12301 tttaaataga aagtataact atgttcatgg tgagtttcaa tttaaaataa gctactttaa
    12361 gcaaattgaa gatataatat actccagtaa taatattagg ctatatacta acatttccaa
    12421 tatgtctgta ttgaaattga tgtcagaatc aagtgtgggt atattgccga cgtggggaga
    12481 tacttatgga tattcagtgc ttgaaatgca atcctttggt ttgcctgtta taacatctaa
    12541 tgtcagagct ctacctgaaa taaaccataa tgatcttatt tttaatgttg acactaatat
    12601 tttaggggag attgttattg atagttttga acagcgtgat gctatttcag aaaaaattat
    12661 cgatggagta aaaaagaaga tggaatatat cttgttgggt gataacaaat tgcatatgtt
    12721 ttctgaatgg tcaattgata acattagaac gaaccatgat ccagatatct tttataaaaa
    12781 aatattaaat atcatcgatg aaaaacgtta actacttagt gtattaatac tatagttaaa
    12841 tagccatttt tgatatatat cagttagcaa tatataactt acttctatac acattcgtgc
    12901 aacttgagat aatatctcat tcatattcaa gtcgcgcata catcgcggtg aacaccccct
    12961 gacaggagta aacaatgtca aagcaacaga tcggcgtcgt cggtatggca gtgatggggc
    13021 gcaaccttgc gctcaa
//

</pre>