<pre>LOCUS       EU296403               14804 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Escherichia coli serogroup O168 O-antigen gene cluster, complete
            sequence.
ACCESSION   EU296403
VERSION     EU296403.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14804)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 14804)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..14804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O168"
     misc_feature    1079..13268
                     /note="O-antigen gene cluster"
     gene            1079..2350
                     /gene="wzx"
     CDS             1079..2350
                     /gene="wzx"
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ACD36992.1"
                     /translation="MFRQINFKNLEIMKQILISFSFRLMGPVIMLLTTPQLLSTLGNV
                     EYGVWSLLITTISWLTICDFGISNQIRNESKILIVQDNKESIREIYTAAVIISFFITL
                     LCCVIISFINIDLIYKLFLSCVFLFAIETNICRALCLSLNKPELASAVTNIPNLFLLI
                     YVFCYSSFNINNVMDIYCYGSAMMFVSIFIILYKFNIKLFWPVFHPSYLRFSIMKYSL
                     LRGGGGFFMVQLVSMGLIISDRYIISSIFGVEDVTKYDLIFKVSNFCIMFFSIVNMVL
                     WSKFTEFWMQGFYTKIKVIFKVFDLCFLLLVIVLILILNHIHWIFELWLNVTNENYNE
                     VYITGIVLYLVGYYWMSAYSSFLNGISIIKKQFIIQGGGLLIKAITLLIAMNILKVFK
                     VEYIILLGGACLFIVGFLFRKCAHDKINSCI"
     gene            2325..2930
                     /gene="wfeU"
     CDS             2325..2930
                     /gene="wfeU"
                     /note="acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeU"
                     /protein_id="ACD36993.1"
                     /translation="MIKSIVVYSFRIYYFINRWITTKINKLRLKANSVELGDSVMING
                     ILHIVNKGEIKIGNGVKINSSLRYNPISHHPCSFYTSENAVIEIGDKSGLSGCTLYSV
                     KKIHIGKRVLIGAGVNIYDTDFHSLLVDERLNGDSNIKSSPVYIYDDCFIGANVTILK
                     GVTIGAGSVVAACSVVTKNIGENELWAGNPARYIRDTIYGE"
     gene            2872..3648
                     /gene="wfeV"
     CDS             2872..3648
                     /gene="wfeV"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeV"
                     /protein_id="ACD36994.1"
                     /translation="MNYGQAIQLGILEILYMANDKITVITINYNNAEGLSKTIASVEK
                     QTNKEFEYLIIDGGSEDNSIEHAKKSVICTKIISEKDNGIYDAMNKGASLAKGEYIIF
                     TNSGDELRKNSIEILMNVLRETNTLYDIYHGILAFKKNDKVIYYRGRKSDMLNSSMIE
                     HPTVLMRKSTFNELKGFDNKWRYVADYDLMLRAYFTKRRFFFIEEVIADFDVSGVSSV
                     SIKAPLESLRLQRKYNLINTFEYFSKLFFIKMKKAVSCLR"
     gene            3636..4751
                     /gene="wfeW"
     CDS             3636..4751
                     /gene="wfeW"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeW"
                     /protein_id="ACD36995.1"
                     /translation="MLKVILLSSINPEQKTGYYNAVNDRVKSLKKEKGIKIWSYSIYK
                     STSLIRFSIKKNENHFFIEYPKAFPSHGLFSFITLWFIRCVILFLWVIIRPNMIHVHW
                     GYPLAYAAVIIKRLTKKTKVIVTFHGSDVHTHPARSKEIFVKTCNLCKRVDHVTAVSE
                     NLLLQVRNKFSVSESKSSITYNGVNLDETNCSFSSSNGLTFSFFGNLNMTKGADRIIP
                     LYNALSGLIEENFTFYVGGEGPCYKEIKQYIIVNGISNIKLLGFMPRADVKRIMRKSN
                     CVFILSRNEGLGISAIEALAYSQLCIAPDIGGLSEVFKENPELLIKNDFNPSDYANKF
                     ISCHKDKFRVNRTYIFKNFSMKNIIQKEITLYNEIMK"
     gene            4767..5999
                     /gene="wzy"
     CDS             4767..5999
                     /gene="wzy"
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ACD36996.1"
                     /translation="MNCKFNIWDIQLGLILISVLSYAFLNLGFLPLNISHLIILFVLI
                     TLSVNLLISQSIKITGLFLTLFVSFILLSAYSLLKYYDVQKVKNIINFFIFFSVAAIV
                     INNCSADKIIKFYYKLTKIFIFFALLQWVLYYLNIGTLYTYSFLGLKEVNISTSGYLI
                     RLFSIASEPAALCGILLPAIYLSINRIVNKGKEVTLSYSVIVLFVILNTFSLVGYIYI
                     IICLIVALYVGNKISLSKIFIFTICLALLVFILFQSDSIQQRLNEITSLDKMASSDNL
                     SVIAIYSNLQIALISLSDNLIFGGGIFSHPYTYDHYISQLYAGGGPRMELNKDDAASL
                     YIRALSETGILGFCILNGLIIYLMKRCDKSKINYPYNIAFTIAFALLGIRAGSVNYII
                     IWFYFFSAIRFVNEGRLK"
     gene            5996..7117
                     /gene="wfeX"
     CDS             5996..7117
                     /gene="wfeX"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeX"
                     /protein_id="ACD36997.1"
                     /translation="MKMLSFLECMNSTYGGPAFSLPSLLHHLEKNYLVQSTVISLKKK
                     NEVYENNVAECLGINPIILNQVGPRKLKLTHEIAKVKKIMQDCDIIHTNNLWNYFSYL
                     PYNLSKNSKKPNVISVRGSLYAWSLEQSRLLKKISWFLFQKKALDSAGFIHVTCNDEL
                     TALRELGIKNKIVVSQHGVDVPDLIDKVPDSFYDKYKLSHNKKYFLFMSRLHTKKGLD
                     MLFNVWSRISHHYPDWELLVAGPDYGNYIPKITKLNAVKYMGMLSGEEKEYCFSIAQF
                     FVLPSYTENFGVVIGEAMARTVPVLTSTNTPWKDVNTNNAGVCFDLSTENLKRELEFF
                     FNLSDSTRKEMGRNARELIVRNYSWERVSYPFYDALKSL"
     gene            7129..8247
                     /gene="gmd"
     CDS             7129..8247
                     /gene="gmd"
                     /note="GDP-mannose-4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gmd"
                     /protein_id="ACD36998.1"
                     /translation="MKKALITGITGQDGSYLAEFLLDKGYEVHGIKRRASSFNTERVD
                     HIYQDRHNQNPNFFLHYGDLTDTSNLIRLIKEIQPDEVYNLGAQSHVAVSFESPEYTA
                     DVDAMGTLRLLEAIRICGLEKKTRFYQASTSELFGLVQEIPQRETTPFYPRSPYAVAK
                     MYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANISQGIEKCLYLG
                     NMDSLRDWGHAKDYVRMQWMMLQQDHPEDFVIATGKQISVREFVRMSAKEVGLELEFS
                     GQGVDEIATVVNKTSDCAIGVSIGDVIVRVDPRYFRPAEVETLLGDPAKAKKVLGWEP
                     EITVEEMCAEMVASDLAKAKQHALLKSHGYDVAVSLER"
     gene            8251..9216
                     /gene="fcl"
     CDS             8251..9216
                     /gene="fcl"
                     /note="GDP-L-fucose synthetase"
                     /codon_start=1
                     /transl_table=11
                     /product="Fcl"
                     /protein_id="ACD36999.1"
                     /translation="MTKKRIYVAGHRGMVGSAICRQLSLRDDIELVVKTHKELDLTVQ
                     KEVDAFFEQEKVDQIYLAAAKVGGIYANNTFPAEFIYQNLMIESNIIHSAHKAGIQKL
                     LFLGSSCIYPKFAEQPMNESALLTGILEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGINDNFHPENSHVIPALMRRFHEAKESGAPEVIVWGTGTPMREFLYVDDMA
                     AASVHVMELDEAIYQQNTQPMLSHINVGTGVDCSIREMAETMASVVGYQGKIVFDVTK
                     PDGTPRKLMDVTRLKNLGWQYQYNLHEGLSLTYKWFIENINSFRG"
     gene            9219..9680
                     /gene="gmm"
     CDS             9219..9680
                     /gene="gmm"
                     /note="GDP-mannose mannosyl hydrolase"
                     /codon_start=1
                     /transl_table=11
                     /product="Gmm"
                     /protein_id="ACD37000.1"
                     /translation="MNKRLERELFKTIVEHTPLISIDLIIRNDKGEALLGQRLNRPAQ
                     NYWFVPGGRIYKDESFEIAFKRITFEEVGVQISLNDALFLGVYEHFYNDNFSEAEFST
                     HYVVHGYEIQLKPQQLHLPTVQHNSYKWFDVVTLLNSTTVHQYTKNYFIPR"
     gene            9686..11092
                     /gene="manC"
     CDS             9686..11092
                     /gene="manC"
                     /note="pyrophosphorylase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManC"
                     /protein_id="ACD37001.1"
                     /translation="MLLPVVMAGGSGTRLWPLSRTLYPKQFLSLNSRLTMLQETLRRL
                     DKVEHKPALVICNESHRFIVAEQLRKEGLKHSGILLEPVGRNTAPAVALAALQAMVTG
                     DDPILLVLAADHEIQNEDNFIDVVLAAKNFAEQGKLVTFGIVPTSPETGYGYIKSGEY
                     LDGKGYKVAAFVEKPELHVAQQYISDGGYLWNSGMFMFRASVFIDELKKFRPDILASC
                     QRSLSSSIQDLDFIRLDNASFSCCPEESIDYAVMEKTAEAVVVPLNAQWSDVGSWSAL
                     WEISSKDQSGNAIRGDVLVEDATEGYLYSQHRLIGAVGIKDLVVVETKDAVLVAHKDK
                     VQQVKNIVAQLKKNNRTEYLQHREIFRPWGSHDTIAEGPRFQVKHVIVLPGHITAKQI
                     HYHRTEHWIVVSGTAKVHLEDKTYLVSENESTYIPVGVPHAIENPGKIPLEIIEVRSG
                     VYLEEDDVIRVSSSGVGY"
     gene            11092..11838
                     /gene="wfeY"
     CDS             11092..11838
                     /gene="wfeY"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfeY"
                     /protein_id="ACD37002.1"
                     /translation="MRISIITATYNSEKTLLDTLLSLEKQTYPDIEYIVVDGASNDNT
                     IKLIKSNCTKVSKIICEPDKGIYDALNKGIQAASGDVIGFLHSDDLLAYDDAIADIAK
                     TFEITGCDAIYGDLEYVAQNDTTKRIRLWKSGSFSRFKMKVGWMPPHPSFYMKRKCYR
                     QFGCFSLDYRISADYDSLLRYILKQRISIEYLPKVLVKMRVGGISNRSVSSMVKKSME
                     DIRVMNQNGIIWPIALVYKNISKLPQFIKK"
     gene            11844..13268
                     /gene="manB"
     CDS             11844..13268
                     /gene="manB"
                     /note="phosphomannomutase"
                     /codon_start=1
                     /transl_table=11
                     /product="ManB"
                     /protein_id="ACD37003.1"
                     /translation="MLNVKKIINDSNIAFGTSGARGLVIDFSHDVCAAFTHAFLSVID
                     DKYNFNKVALAIDNRPSSYEIAQACAFAIKQHGFTVEYHGVIPTPALAHYSMQKNIPC
                     IMVTGSHIPFDRSGLKFYRPDGEITKEDELAIINSEYTFPPVGVLPHLETSTQGADYY
                     LERYVSLFNPEILKGKRIGVYEHSSAGRDLYAPLFNQMGAEVISLGRSDEFVPIDTEA
                     VSDEDRILAREWSKKYNLDAIFSTDGDGDRPLVADENGEWLRGDILGLLTAIELNIKA
                     LAIPVSCNTAIEESNKFASIQRTKIGSPYVIAAFADLAKQFDSVAGFEANGGFLLASD
                     LQINGKELKSLPTRDAVLPALMLLIASRNSTISQLINNLPQRFTWSDRVKNFPSDSSQ
                     QIIKNAISSPNNFFNSLGYESLSCSAIDETDGARFTLNNGDIIHLRPSGNAPELRCYA
                     EASDENQAKQYVTNVLGNITSLIS"
ORIGIN      
        1 attgtggctg cagggtcaaa gaaatcctcc tggtaactca cgcgtccaag aacgcggtcg
       61 aaaaccactt cgacacctct tatgaattag aatctcttct tgagcagcgc gtgaagcgtc
      121 aactgcttgc ggaagtgcag tccatctgtc caccgggcgt gaccattatg aacgtgcgtc
      181 agggcgaacc tttaggttta ggccactcca ttttatgtgc acgacccgcc attggtgaca
      241 atccatttgt cgtggtgctg ccagacgttg tgatcgacga cgccagtgcc gacccgctgc
      301 gctacaacct tgcagccatg attgcgcgct tcaacgaaac gggccgcagc caggtgctgg
      361 caaaacgtat gccgggtgac ctctctgaat actccgttat tcagaccaaa gaaccactgg
      421 atcgtgaagg taaagtcagc cgcattgttg aattcatcga aaaaccggat cagccgcaaa
      481 cgctggactc agatattatg gccgtgggcc gttatgtgct ttctgccgat atttggccgg
      541 aacttgaacg cactcagcca ggggcatggg gacgtattca gctgactgat gccattgccg
      601 aactggcgaa aaaacagtct gttgacgcca tgctgatgac tggtgacagc tacgactgcg
      661 gtaaaaaaat gggctatatg caggcgttcg tgaagtatgg attacgcaac ctaaaagaag
      721 gggcgaagtt ccgtaaaggg attgagaagc tgttaagcga ataatgaaaa tctgaccgaa
      781 tgtaacggtt gataagaaaa ttataacggc agtgaagatt cgtggcgaaa gtaatttgtt
      841 gcgaatattc ctgccgttgt tttatataaa caatcagaat aacaacgagt tagcaatagg
      901 attttagtca aagttttcca ggattttcct tgtttccaga gcggattggt aagacaatta
      961 gcgtttgaat ttatgagggc tttgcggggt tagatgcaga gttcgtgaca tccattgaga
     1021 atctacgcag tgcattggta gctgtaagcc aggggcggta gcgtgtgcaa ataagaatat
     1081 gttccgacaa ataaatttca aaaatctaga aataatgaaa cagattctaa tttcattttc
     1141 ttttagatta atgggtcctg ttatcatgct tctgaccacg ccacaattgt tgtcgacttt
     1201 gggaaatgtg gaatatggag tttggtcttt attgattaca acgattagtt ggttgacaat
     1261 atgcgacttt ggcatatcaa atcagatacg aaatgaaagt aagattttaa ttgttcaaga
     1321 taataaagaa agtatacgag agatatatac agcggcagtt ataatttcat tctttataac
     1381 attattatgt tgtgtgatta taagttttat taatatcgac ttgatatata aattgttttt
     1441 atcttgtgtt tttttatttg ctatagaaac aaatatttgt agagccctat gcctttcatt
     1501 gaataaacct gaattggcct ctgcagtaac taatattccg aatttatttt tattgatata
     1561 tgtgttttgt tattcgtcat ttaacataaa caatgtgatg gatatatatt gttatggctc
     1621 tgccatgatg ttcgtatcaa tttttattat tttatataaa tttaatatta agttgttttg
     1681 gccagttttc cacccttctt acttaagatt ttctatcatg aaatactctc tacttagagg
     1741 tggtggtggt ttctttatgg ttcaattggt ttcgatgggg ttaataataa gtgatagata
     1801 tataatatca tctatatttg gtgttgaaga tgtcacaaaa tatgatttga tatttaaggt
     1861 gtcaaatttt tgcataatgt ttttttcaat tgtaaatatg gttctttggt cgaaatttac
     1921 agaattttgg atgcaagggt tttatacaaa aatcaaggtt atctttaaag tgtttgactt
     1981 atgtttttta ttgttagtta tcgtcctaat tttgatttta aaccatatac actggatttt
     2041 tgaattatgg ctaaatgtaa ctaatgaaaa ttacaatgaa gtttatatta cagggattgt
     2101 cctttatttg gttgggtatt attggatgtc agcttattcc tcttttttaa atggtataag
     2161 catcataaaa aaacaattta ttatccaggg tggagggctt ttgatcaaag ctattacttt
     2221 attaattgct atgaatatct tgaaagtatt taaggttgaa tatattattt tgctgggtgg
     2281 tgcgtgtcta tttattgttg gttttttatt caggaagtgt gcgcatgata aaatcaatag
     2341 ttgtatatag ctttcggatt tattatttca tcaatagatg gattacaaca aaaataaata
     2401 aattgaggtt gaaagctaac tctgtcgaat taggtgacag cgtaatgatt aatggaatat
     2461 tgcatatagt taacaagggg gaaattaaaa ttggtaacgg tgtcaagatt aattcttcat
     2521 tacgttacaa tccaatttca catcatccat gttcatttta cacttctgaa aatgcggtga
     2581 ttgagattgg ggataaatcc gggttgtcgg gttgtacctt atattctgtt aaaaaaatcc
     2641 atattggaaa aagagtgttg attggtgcag gcgtaaatat ttatgataca gattttcatt
     2701 ctttactcgt ggatgagaga ttaaatggtg atagtaacat taaatcctct ccagtttata
     2761 tatacgatga ttgttttatt ggagctaatg ttacaatttt gaaaggtgtt acaatagggg
     2821 ctggcagtgt tgttgctgct tgctcggtag taacgaagaa tataggggaa aatgaactat
     2881 gggcaggcaa tccagctagg tatattagag atactatata tggcgaatga taaaattacc
     2941 gttataacta taaattacaa taatgcagaa ggcttatcaa aaacgattgc ttcagttgaa
     3001 aaacaaacta ataaagaatt tgagtatcta attattgatg gtggttctga ggataattca
     3061 atagaacatg ctaagaaaag tgtaatatgc acaaaaataa tatctgaaaa agataatgga
     3121 atctatgatg caatgaataa aggagcctca cttgctaaag gtgagtatat catttttaca
     3181 aactctggtg atgaattaag aaaaaatagc attgaaattt tgatgaatgt attgagagag
     3241 acaaatacat tatacgatat ataccacggg attttggctt tcaaaaaaaa tgataaagtt
     3301 atttattata gaggtcggaa aagtgacatg ttgaacagtt caatgattga acatcctact
     3361 gtgttaatgc gtaaaagcac atttaatgaa ttaaaaggat ttgataataa gtggcgatat
     3421 gtagctgatt atgatttaat gttacgggca tattttacaa aaagaagatt tttttttatt
     3481 gaggaggtga ttgctgattt tgatgtatca ggtgtatcta gtgtgagtat taaagctcct
     3541 ttggagtctt tacggttaca acggaaatat aatttaatta atacatttga atattttagc
     3601 aagttatttt ttatcaagat gaaaaaggct gtatcatgct taaggtaatc cttctttcat
     3661 caatcaaccc agaacaaaaa acaggctact ataatgctgt caatgaccgt gtaaaatctc
     3721 taaaaaaaga gaaaggaata aaaatttggt catattcgat atataaaagc acgtcattga
     3781 taagattctc aataaaaaaa aatgaaaatc atttttttat agaatatccc aaagcatttc
     3841 cttcgcatgg tttattttca ttcataactc tctggtttat aagatgtgtt attttatttc
     3901 tgtgggttat aataagacct aacatgatac atgtgcattg gggatatccg cttgcatatg
     3961 ctgctgtaat tataaaaaga ttaaccaaaa aaacaaaagt cattgtgact ttccatggct
     4021 ctgatgtgca tacacatcct gccagatcga aagaaatttt cgtaaaaact tgtaatttat
     4081 gtaaaagagt tgatcatgtt acggctgtga gtgagaactt attattgcaa gtaagaaata
     4141 agttttcagt gtcagaatcg aaatcatcta taacatataa tggagttaac ctagatgaaa
     4201 ctaattgttc tttttcatca agtaatggat tgacgttttc gttttttggg aatctaaata
     4261 tgactaaagg tgctgatagg ataattccac tttataatgc actttcagga ttaattgaag
     4321 agaactttac attttatgtt ggcggggaag gaccttgtta taaggaaata aagcaatata
     4381 ttatcgtaaa tggaatctct aatattaaat tgctcggttt tatgcctcgt gctgatgtaa
     4441 aacgaataat gcgcaaaagt aattgtgtct ttatattaag tcgtaatgaa gggttgggta
     4501 ttagtgcaat tgaagcatta gcttatagtc aattatgtat agcacctgat ataggtggat
     4561 tatctgaagt ttttaaagag aatccagagt tattaattaa aaatgatttt aatccatcag
     4621 actatgctaa taagtttatt agttgtcaca aagataaatt cagagttaat cgtacatata
     4681 tatttaaaaa cttttctatg aaaaacataa ttcagaaaga aataacctta tataatgaaa
     4741 taatgaaata atgaaacagg cacaaaatga attgtaaatt taacatttgg gatattcagt
     4801 taggtttgat attaatatca gtactatctt atgctttttt aaacttgggt tttttaccat
     4861 taaatatttc ccatctaata attttatttg tgttgataac gctaagtgtt aatctgttaa
     4921 ttagccaatc aattaaaata actggattat ttttaactct ttttgtgtcc tttattttac
     4981 tttctgctta ttcattattg aaatattatg atgttcaaaa agtaaaaaat ataattaatt
     5041 tttttatttt ttttagcgtt gctgctatag tgataaataa ctgcagtgct gataaaataa
     5101 taaaatttta ttataaatta actaaaattt ttatattttt tgctttactt cagtgggttc
     5161 tttattatct caacattgga acactatata catattcttt tcttggttta aaggaagtta
     5221 atatttcaac aagtggatat ttaattcggc tattctctat agctagtgag cctgctgcat
     5281 tatgtgggat attgcttcca gctatatatc tatctataaa tagaatagtt aataagggta
     5341 aagaagttac tttatcatat tcagtaattg ttttatttgt tattttaaat acattttcgt
     5401 tggttggcta tatatatata atcatttgtt taatagtagc gctatatgta ggtaataaaa
     5461 tctcattaag taaaatcttt attttcacaa tttgtttggc attgcttgtc tttattcttt
     5521 ttcaatcgga tagtatccag cagcgtttaa atgaaataac tagtctagat aaaatggcta
     5581 gctcagacaa tctttctgtt atagcaattt attcaaattt gcaaatagct ttgataagtc
     5641 tcagtgataa tcttatattt gggggaggaa tatttagtca tccttataca tacgatcatt
     5701 atatcagtca attgtatgct ggtggaggac cacgcatgga attaaataag gatgatgcag
     5761 cttctcttta catacgtgca ttatcagaaa cgggaatttt aggtttttgt attttgaatg
     5821 ggttgattat atatctgatg aaacgctgcg ataaatctaa aatcaactat ccatataaca
     5881 tcgcttttac aattgcattc gcattgcttg gtattagagc tggtagtgtt aattatataa
     5941 tcatttggtt ctattttttt tccgctatac ggttcgtaaa tgaaggaagg ttaaaatgaa
     6001 aatgttatcg tttttggaat gtatgaatag cacttatgga gggccagcat tttcacttcc
     6061 ttctttactt catcatttag aaaaaaacta tcttgttcaa agtacagtta tttcgcttaa
     6121 aaagaagaat gaagtttatg aaaataatgt tgctgaatgt ttgggaataa acccgattat
     6181 attaaatcag gttggcccaa gaaagttaaa attaacacac gagatagcaa aagtaaaaaa
     6241 aataatgcaa gattgtgata taattcatac gaacaacttg tggaattatt tttcttattt
     6301 gccctataat ttatcaaaaa acagtaaaaa gccgaatgtt atttccgtca gaggcagttt
     6361 atatgcttgg tctcttgaac agtcgagact gttaaaaaaa atctcatggt ttctttttca
     6421 aaagaaagca ttggattcag ccggttttat acatgttact tgtaacgatg aattaacagc
     6481 tttacgagaa ttaggtatta aaaataaaat agttgtttca caacatggtg tggatgtacc
     6541 tgatttgata gataaagttc cagatagttt ttatgataaa tataaattat cgcataataa
     6601 aaaatatttt ctatttatgt ctcgactaca tacaaaaaaa gggctagata tgttgtttaa
     6661 tgtatggtcg aggatctctc accattatcc tgattgggaa ttacttgttg ctggtcctga
     6721 ttatggaaac tatataccaa aaataactaa gttaaatgca gtaaaatata tgggaatgct
     6781 aagtggggaa gagaaagaat actgtttttc tatagctcaa ttttttgttc taccttcata
     6841 cactgaaaat tttggcgttg taattggtga ggctatggca agaaccgtac ctgttttaac
     6901 ttctactaat acaccatgga aagatgttaa taccaataat gcaggtgtat gtttcgattt
     6961 atcaactgaa aatttaaaaa gagaattgga gttttttttc aatttatctg attcaactag
     7021 aaaagaaatg ggaaggaatg cgagagaatt gatagttaga aactattcat gggaacgagt
     7081 tagttatcct ttttacgatg cacttaaatc actttgaggt ttaatagaat gaaaaaagca
     7141 ttaatcacag gcatcaccgg tcaagatgga tcttacttag cagaattcct tcttgataaa
     7201 gggtatgaag tgcatggcat taaacgtcga gcatcttcat ttaatacgga gcgtgttgat
     7261 catatttatc aagatcgcca caatcaaaat ccaaatttct ttcttcatta cggtgatttg
     7321 acagatactt cgaacttgat acgtctcatt aaagagatcc aaccagacga agtttataac
     7381 cttggcgctc aatctcatgt agcggtttca ttcgaatcac ctgaatatac tgctgacgta
     7441 gatgcaatgg ggactttacg tctgctggaa gcgattcgta tttgtgggtt agagaaaaaa
     7501 acacgcttct atcaggcatc tacttctgaa ctcttcggct tagttcagga aatcccgcag
     7561 cgagaaacaa caccattcta ccctcgttcg ccttatgctg ttgctaagat gtatgcatac
     7621 tggattactg taaactatcg tgaatcctat ggaatgtacg cctgtaatgg tattctcttc
     7681 aaccatgaat ccccgcgtcg tggtgaaaca ttcgtaacac gtaaaatcac gcgtgctatt
     7741 gcaaatattt cgcagggaat cgaaaaatgt ctctatcttg gcaatatgga ttcactgcgc
     7801 gactggggac atgcgaaaga ttacgtgcgt atgcagtgga tgatgttgca gcaagatcac
     7861 ccagaggatt ttgttattgc aacaggcaaa caaatttccg tacgtgaatt tgttcgtatg
     7921 tcagctaaag aagtcggtct ggagttagaa ttctccggac aaggtgttga tgaaatagca
     7981 actgttgtga ataaaacatc tgactgtgct attggtgtta gtattgggga tgtgatcgtt
     8041 cgtgttgatc ctcgttattt ccgtcctgca gaagttgaaa ctcttcttgg tgatccagca
     8101 aaagcgaaaa aagtattggg ttgggaacca gagattacag ttgaagagat gtgtgcagaa
     8161 atggttgcca gtgatctggc gaaagcaaaa cagcatgcac tactgaaaag ccatggttac
     8221 gatgttgcag tttctctgga gcggtaaggt atgacaaaaa aacgtatcta cgttgctggt
     8281 caccgaggta tggttggctc tgctatttgc cgtcaattat cactgcgtga tgatatcgaa
     8341 ttagtggtca aaacacacaa agaactcgat ctaaccgtac agaaggaagt tgatgcattt
     8401 tttgagcaag agaaagttga tcagatttat cttgctgcgg ctaaagtcgg tggcatttat
     8461 gccaacaata catttccggc agaattcatc tatcagaatc tcatgattga gagtaatatt
     8521 attcattcag ctcacaaggc cggaattcaa aaattacttt ttttaggctc aagctgtatt
     8581 tatcctaaat tcgcagagca gccgatgaac gagtctgcgc ttttaacggg catacttgag
     8641 ccaactaatg agccatacgc aattgctaaa attgcgggca taaaattatg tgaatcttat
     8701 aaccgtcaat atggccgtga ttatcgcagt gtaatgccta ctaatcttta tggcataaat
     8761 gataatttcc accccgaaaa ctctcatgtt attccggcac tcatgcgtag attccatgag
     8821 gcaaaagaga gtggtgcacc agaagttatt gtctggggaa caggaacacc gatgcgtgag
     8881 tttttatatg ttgatgatat ggctgccgca tctgttcatg taatggaact tgacgaagca
     8941 atttatcaac aaaatacaca gcctatgtta tctcatatta atgttggtac gggtgtagat
     9001 tgttccatac gtgaaatggc tgaaacaatg gcctctgtgg tgggttatca aggtaaaatt
     9061 gtttttgatg ttactaaacc tgatggcact ccgcgcaaac ttatggacgt tacccggctc
     9121 aaaaatttgg gctggcaata tcaatataac ttacatgaag gcttatcatt aacatataaa
     9181 tggtttattg agaatattaa ttcttttcgg ggatagttat gaataagaga ttggaacgtg
     9241 agctatttaa aacaatagtt gagcatactc ctctaatctc gattgatctc ataattagaa
     9301 acgataaagg agaggcgctg ctggggcagc gcctgaatcg cccagcacaa aattattggt
     9361 ttgtgccagg agggcgaatt tataaggatg agtcattcga gattgcattt aagcggataa
     9421 catttgaaga ggtgggcgtt caaattagtc ttaatgatgc cttatttcta ggggtgtatg
     9481 aacattttta caatgataat ttttctgaag cagaattttc tacacactat gtagtgcatg
     9541 gatatgaaat ccaacttaaa cctcagcaac ttcacctacc aacggtccag cataattcct
     9601 acaagtggtt tgatgtagta acgttgctta atagcactac agttcatcaa tataccaaaa
     9661 attattttat accaaggtaa tagatatgct acttcccgtt gtcatggccg gtggttctgg
     9721 taccagatta tggcctcttt cacgtacact ttatccgaaa caatttctgt ctttaaatag
     9781 tcgtttaacc atgttgcaag agacattgcg gcggcttgac aaggtagaac ataaacccgc
     9841 tttggtcatt tgtaacgaat cacatcgctt tatcgttgct gaacaattgc gtaaagaggg
     9901 tttaaagcat agcggtattt tgcttgagcc tgttggtcgt aatactgcgc ctgctgtagc
     9961 actcgcagca ctacaggcta tggtaactgg agatgatcct attctgttgg ttcttgctgc
    10021 tgatcatgaa attcagaatg aggataattt cattgatgta gttcttgcag ccaagaattt
    10081 tgcagaacag ggtaagcttg ttacgtttgg tattgttcca acatccccag aaacgggcta
    10141 tggttacatt aagtcaggtg aatatctgga tggaaaaggt tacaaagttg ctgcttttgt
    10201 tgaaaaacca gagcttcacg tagctcagca gtatatatca gatggcggtt atctttggaa
    10261 tagtgggatg tttatgttca gagcatctgt atttatcgat gaactgaaaa aattccgacc
    10321 agatatttta gccagttgcc aacgctcctt gtcctcttcg atacaagatt tagattttat
    10381 ccgtctggat aatgcttcat tttcttgctg tcctgaagag tctattgact atgcagttat
    10441 ggaaaaaaca gccgaagctg tcgtcgttcc attaaatgca caatggagtg atgttgggtc
    10501 atggtctgca ttgtgggaaa taagttcaaa agatcaaagc ggtaatgcca ttcgtggtga
    10561 tgtattggtt gaagatgcta cagaaggtta tctttattcg cagcatagac ttattggtgc
    10621 cgtgggcata aaggatttgg ttgttgttga aacgaaagat gccgtattag ttgctcataa
    10681 agataaagtt cagcaagtta aaaacatcgt cgctcaactt aaaaagaata atcgcacaga
    10741 atatttacag catcgggaaa tatttcggcc ttggggcagt catgatacta tagctgaagg
    10801 gccgcgcttt caagtgaaac atgtgattgt attacctggc cacattaccg ctaagcaaat
    10861 tcattaccat cgtactgagc attggattgt tgtctcgggg acagctaaag tccatcttga
    10921 agataagact taccttgttt ctgaaaatga atcaacatat atacctgttg gtgttccaca
    10981 tgccattgaa aatcctggca agatcccgct tgaaataatc gaggtcagat caggagtcta
    11041 cctggaagag gatgatgtta taagagtttc ttcttctgga gtaggatact aatgcgaatt
    11101 tctattatca cagctactta taatagtgaa aaaactctcc ttgatacatt actttctcta
    11161 gaaaaacaaa cgtatccaga tatcgaatat atagttgtag atggagcatc gaacgataat
    11221 acaattaagc tgattaagag taattgtaca aaagtttcaa aaatcatttg cgaacccgat
    11281 aagggcattt atgatgctct gaataaaggt attcaagcag cttcgggtga tgttattggt
    11341 tttttacatt ctgatgattt attagcttat gatgatgcta ttgcagatat agcaaaaaca
    11401 tttgaaatta cagggtgtga tgctatttat ggcgatttgg agtatgttgc ccaaaatgat
    11461 acgactaaac gtattagatt atggaaaagt ggttcattta gtcgtttcaa gatgaaagtg
    11521 ggttggatgc cgccacaccc atcattttat atgaaacgca aatgttatag gcagtttggt
    11581 tgtttttctt tagattatcg tatatctgct gattatgact cattattacg atatatttta
    11641 aagcaacgta tttcaataga atatttacca aaagtcttag tgaagatgcg tgttggtgga
    11701 attagcaatc gttcagtctc ttcaatggta aagaagtcaa tggaagacat ccgtgttatg
    11761 aaccagaatg gtattatctg gccaatagct ttagtgtata aaaatatatc caaacttcct
    11821 caatttatta aaaagtaatc atcatgttaa atgttaaaaa aatcattaac gatagtaata
    11881 ttgcgttcgg tactagtggt gctcgcggtt tagtgatcga tttttcccat gatgtttgtg
    11941 ctgcgttcac tcatgcgttt ctttctgtta ttgatgacaa atacaatttt aataaagttg
    12001 ccttagcaat tgataaccgc ccaagcagtt acgaaatcgc tcaggcatgc gccttcgcta
    12061 tcaaacaaca tgggttcact gtcgaatatc atggtgtaat tccgactccc gcattagctc
    12121 attattcgat gcagaaaaac attccctgca taatggtcac agggagtcat atcccttttg
    12181 atcgtagtgg cttaaaattc tacagacctg atggtgaaat cactaaagag gatgagctcg
    12241 caattataaa tagtgagtat acatttccgc ctgtaggtgt attacctcat cttgaaacaa
    12301 gcactcaagg tgcagactac taccttgagc gttatgtttc tctttttaat cccgagattt
    12361 taaaggggaa aagaataggg gtatatgaac attctagtgc gggacgcgat ttatatgctc
    12421 ctctttttaa tcaaatgggt gcagaggtca tttccctcgg caggagtgat gagttcgttc
    12481 cgattgatac ggaagcagta agcgatgaag atcgtatact tgcaagagag tggtctaaaa
    12541 aatataatct tgatgctatt ttctcaacag atggcgatgg tgatcgtcct ttagttgctg
    12601 atgaaaatgg tgaatggtta agaggcgata ttctgggact acttactgct attgaactta
    12661 atatcaaggc gttggctatt ccagtgagtt gtaatacagc aattgaagag tctaataaat
    12721 ttgcaagtat acaacgaacg aaaataggtt ctccttatgt aattgcagca tttgcagatc
    12781 ttgctaagca atttgattca gtcgcaggtt ttgaagctaa tggtggtttt ctccttgcct
    12841 ccgatttaca aattaatggc aaggaattaa aatcgttgcc tacacgagat gctgtgttac
    12901 cagcattaat gctcttaata gcttctcgca atagtactat ttctcaactg attaataatc
    12961 ttcctcagcg attcacttgg tcagatagag ttaaaaactt cccttcagat tcaagtcaac
    13021 aaattataaa aaatgccata tcgtcaccca ataatttctt taatagttta ggttatgaat
    13081 cattatcctg ttccgctatt gatgaaacgg atggtgcaag atttacttta aataatggtg
    13141 atattataca tctccgtcct tccggtaatg ctccagaact ccgttgttat gctgaggcca
    13201 gtgatgaaaa tcaggctaag caatatgtta cgaatgtgct gggaaatatt acctctttga
    13261 tttcttgatg ttataggttt atctacgctt atatgtgtgc gtaggtttga ttacacgtag
    13321 atgccggtat acagaattga agaacggtat ttgttgcatt aatgaaattc agcactacac
    13381 acattcgtgc aacttgagat aacatctcaa tcatattcaa gtcgcgcata catcgcggtg
    13441 aacaccccct gacaggagta aacaatgtca aagcaacaga tcggcgtcgt cggtatggca
    13501 gtgatggggc gcaaccttgc gctcaacatc gaaagccgtg gttataccgt ctctattttc
    13561 aaccgttccc gtgagaaaac ggaagaagtg attgccgaaa atccgggcaa gaaactggtt
    13621 ccttactata cggtgaaaga gtttgttgaa tctctggaaa cgcctcgtcg catcctgtta
    13681 atggtgaaag caggtgcagg cacggatgct gctattgatt ccctcaaacc atatctcaat
    13741 aaaggcgaca tcatcattga tggtggtaac accttcttcc aggacaccat tcgtcgtaac
    13801 cgtgagcttt ctgccgaagg ctttaacttc attggtaccg gtgtctccgg tggtgaagaa
    13861 ggcgcgctga aaggtccttc cattatgcct ggtgggcaga aagaagccta tgaactggtt
    13921 gcgccgatcc tgaccaaaat cgccgcagtg gctgaagacg gcgagccatg cgttacctat
    13981 attggtgccg atggtgcagg tcactatgtg aagatggttc acaacggtat tgaatacggc
    14041 gatatgcagc tgattgctga agcctattct ctgcttaaag gtggcctgaa tctcaccaac
    14101 gaagaactag cgcagatctt taccgagtgg aataacggtg aacttagcag ctacctgatc
    14161 gacatcacca aagatatctt caccaaaaaa gatgaagagg gtaactacct ggttgatgtg
    14221 attctggatg aagcagcaaa caaaggtacg ggcaaatgga ccagccagag tgcgctcgat
    14281 ctcggcgaac cgctgtcgct gattaccgag tctgtgtttg cacgttatat ctcttctctg
    14341 aaagatcagc gcgttgccgc ttctaaagtt ctctctggcc cgcaagcgca gccagcaggc
    14401 gataaggctg agttcatcga aaaagttcgt cgtgcgctgt atctgggcaa aatcgtttct
    14461 tacgctcagg gcttctctca actgcgtgct gcgtctgaag agtacaactg ggatctgaac
    14521 tacggcgaaa tcgcgaagat tttccgtgct ggctgcatca tccgtgcgca gttcctgcag
    14581 aaaatcaccg atgcctatgc cgaaaatccg cagatcgcta acctgctgct ggctccgtac
    14641 ttcaagcaaa ttgccgatga ctaccagcag gcgctgcgcg atgtcgtcgc ttatgcggta
    14701 cagaacggta tcccggttcc gacctttgct gctgcggttg cctattacga tagctaccgt
    14761 gccgctgttc tgcctgcgaa cctaatccag gcacagcgcg acta
//

</pre>