<pre>LOCUS       EU296408               12864 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Escherichia coli serogroup O167 O-antigen gene cluster, complete
            sequence.
ACCESSION   EU296408
VERSION     EU296408.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12864)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 12864)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..12864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O167"
     misc_feature    1774..11472
                     /note="O-antigen gene cluster"
     gene            1774..3039
                     /gene="wzx"
     CDS             1774..3039
                     /gene="wzx"
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ACD37041.1"
                     /translation="MVKTDLTVFKNIISLFSIRFAGYIIPLITLPYLVKVLQPVGFGY
                     LSFGIAVLQYCTLVVNYGFDLSATRKIAQNRENVHYISDIFWNILFLRLFISLVGFLV
                     LVVFIHLFPDLYRIKWILLWGYLSVLGTALFPMWLFQGKEQLGRISAARIFLQISCVP
                     LFFVFVKNTQDDWVASLIYALPQLLISVVAVYIIHKRKWIVLKKIKLATIKKELIDGW
                     HLFISTAAISLYTTTITVVLGIVPGPYSVAIYTSANKILQAAQGLYQPISSAFYPRIN
                     NLIKENKQLAFKTIRKLFKIQFVITICISVGLFLFSNIVVDILYGDDYRNAALILQLL
                     SPIPIVVGCSNIFGIQILLTCGYKKEFSTILLISGLINMILIFPLCYKFNEFGAAFSV
                     LITEIFVTVSMLFVVILKKIPVWESKRNV"
     gene            3032..4120
                     /gene="glf"
     CDS             3032..4120
                     /gene="glf"
                     /note="UDP-galactopyranose mutase"
                     /codon_start=1
                     /transl_table=11
                     /product="Glf"
                     /protein_id="ACD37042.1"
                     /translation="MYDYLIVGSGLFGAVCANELKKRNVKILVIDKRNHVGGNVYTEN
                     INGIQVHKYGAHIFHTNDKYIWEYVNKLVEFNRFTNSPLAYFKGELFNLPFNMNTFYQ
                     LWGVKKPSEAAAIIEEQRKVMLKKEPTNLEEQAISLVGVDIYEKLIKGYTEKQWGRSC
                     KELPSFIIRRLPVRFTFDNNYFSDKYQGIPVGGYTNLIEKMLEGVEVHLGIDYLKERK
                     KYSKIAKKIIYTGPIDEYFDNVLGQLEYRSLKFQTEKMDIPNYQGNAVVNYTEKEIPF
                     TRIIEHKHFDFVESNFTIITKEYPKEWSNGDEPYYPINDEKNMALYRKYKSLADEENV
                     IFGGRLAEYKYYDMHQVIRSALNCVKHI"
     gene            4153..5355
                     /gene="wfdF"
     CDS             4153..5355
                     /gene="wfdF"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfdF"
                     /protein_id="ACD37043.1"
                     /translation="MKLYYICSDFYPSGTGFAISFTNFIQFILDSNKFEHITILTTNS
                     KASLPENFNERVLMQTFDVRGELKLARYFRSSILLDLYSIFKYRRILHMLKCCCIKSD
                     DVFFYEEFYFGNLYKYLKRRFPCNKHIIRVHGTMPEFVTFDRSNRYRKILFKQALELK
                     NNRENKITIATTTSFYIDFINTHILKNQYGTICNVDYIFLPNSIYSNKNIPQTQKSEN
                     MSNSLTLLQLGRMDKGGYFQKGFDDTIKALNYINSDVFLSHRIRLVTIGSGEKKKYFK
                     DKMRDLKNIAFEHYENINNEAVNDLIMQADVILLPSRCEGMSMFAVEAISLGKPIITT
                     RNTGVDDICIEGVNSLKFDMFNYVEYAQAIEKIIKEPHLIRQMGYNAFAVSKENEKKL
                     KANIEAFL"
     gene            5355..6209
                     /gene="wfdG"
     CDS             5355..6209
                     /gene="wfdG"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfdG"
                     /protein_id="ACD37044.1"
                     /translation="MIYRYILNLFPDTLFIRLRYFKRFKRILHLRKPKRFTEKIMWYK
                     LFYHDNLMTICADKYRVREYIKEKGFGDILVPLFKVYNTPDEIKIDELPCQFILKGNN
                     GSETNLVVRDKSKITEKYIRHIVSGWMANNQVITLGKEWCYQNIEFKITVEQLLESEG
                     EDGIDDYKFLCFNGRVHYIWVDKNRFTSHTRTFFDRDWNVINVESDHPAASFSIDKPW
                     GFERMLHIAEDIGKDFPFARVDFYSVNKKVYFGEITFYPWSGMVQFQPDSFDFDMGDK
                     FILPKEML"
     gene            6209..7117
                     /gene="wfdH"
     CDS             6209..7117
                     /gene="wfdH"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfdH"
                     /protein_id="ACD37045.1"
                     /translation="MSKLSVIMSVYSESEKMLRQSIDSVLNQSFRDFEFIIVNDNPER
                     IEIIQLLRYYMEKDNRVKVFSNEINKGLTKSLNIAAKKSNSKYIARMDADDISDITRF
                     DRQLDILEKNDDIDICGSNVKVINVHDNVTGHYRYPERSSKIKGKLFFRDCLCHPATM
                     FRRSFFIEVGGYSEDFKKAQDYELWIKVLINDKGIYNIQTPLLYYRMHSENISTKNID
                     EQHYYSIKAKVLLYSFFMDQQQSLKIIDYIEGQQGNVHIIRVLKFFFVLAGRLNKKIN
                     GFSYIYFFFLLVKRLIYIKIIRRAIF"
     gene            7165..8415
                     /gene="wzy"
     CDS             7165..8415
                     /gene="wzy"
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ACD37046.1"
                     /translation="MNNTLRQYSLVQVFITMYCLFPFYFHNIYIIQTFSLYSLALSFV
                     VYTFFYKVRFVSRSGAITILASTTTFLIFLFMSMLINLSSDMTFVYEQVFGFSKQLLL
                     ALVPFCLYFSFDSDDINYAVHSILLLFIRAISLYVFISVIFLIPEVRNYWIDIIYFSS
                     DQNKELINSASYYTRFGLQGFSGFRHAILCAIALIFSIYLFMCGYKKIKNYWMSVFLV
                     CIGCFLYGRIAIAVILCITLISLFYLLCKFEKLYFIFIVCLCVGTIACVVYMNIDYIQ
                     DSYSLAWIFEPLINFINEGQFTSHSTNDLSSMYIMPNENTMLLGDGKYTNHDGSYYMH
                     TDVGFLRPVFFGGIFFVFFYYMMLILPLILIIKNSGLERRNILTLFLALALIVFELKG
                     ESFISFSAIIFSFILIPIICKRLN"
     gene            8415..9506
                     /gene="wfdI"
     CDS             8415..9506
                     /gene="wfdI"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfdI"
                     /protein_id="ACD37047.1"
                     /translation="MRISLEENSVVFILYQFHIGGVESALINLLENIKKKRVYLIFAM
                     NKINNELLKKIPHNVKIIGLGINNKNICHLIGYTRRLSKRYDFSRTHIVNFSDTLSTL
                     IIQYSLNAKLKTSWVHCNPLTLINSRSFQLYKYLLVRNDKIVCLCESQKNLLIKVMPK
                     AEKKVTIIYNCVDADILSKGAQEKVDVDYKYIIKVARFDERTKDFITLIDAYCKLPKE
                     TQDTYKLVLLGDGPDLENVRNYTRKKNNMQNIFFPGKDVNPFKWIKHSQFLVHSSTSE
                     GFSLVLLEAMLLGKVVIASDCNCGPSDILDNGNAGFLFNVGDVDSLTCIMQNLLVNQD
                     IINIYTNRSLVRGVELQLKANRQVKEYFL"
     gene            9503..10462
                     /gene="wfdJ"
     CDS             9503..10462
                     /gene="wfdJ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfdJ"
                     /protein_id="ACD37048.1"
                     /translation="MSSVSVCIVTFNRKNDLLRCLDNVLKQSHVVSNILIYDNASTDG
                     TTRFLLSKYCAILSPSNIPQKIATLNDINIWLVTATENSGGAGGFHYSVILSREIFDT
                     DYYWLMDDDGYPSKDCLNILMQYAETHNSDYVMPVSIDIDNNEQLSWPTRKKNHAKTE
                     CYKELYESWGDVMDYVTPFNGSLLSKKCIDSVGYVNKDFFIWGDEYEHYWRCKKSRIH
                     PVTIMNAKFYHPSQKLPLQPCFFGLIKLPYVDSPLRMVCLVRNYTYIYKKYDSKLKII
                     LKLISYSWFFLITRKLDAKGFVLYLKSVLDGLRNDFTRHRDFL"
     gene            10570..11472
                     /gene="wfdK"
     CDS             10570..11472
                     /gene="wfdK"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfdK"
                     /protein_id="ACD37049.1"
                     /translation="MTVIAIVVTFNRCALLKKVLHSLLSQSIALNKIIIIDNDSNDDT
                     AKVVHDFSEVDDIFYYYNTGDNLGGAGGFYQGFKIAEQLYYDYLWLMDDDLLPEPDCL
                     EKLIQDRPEGIVQPVRYDLDGACAEISPVEYNLQKIFCRNPKTKTVKEVISTVISDNC
                     REIDIAGVPFEGPLISKSVVNKVGYPNPDFFIFNDDLDYSLRTRSKGFSIKCIVDARA
                     TRLLKNNQKNDLKSWKGYFMLRNHYYILRNYGENKLVKNRVYLIMFYYFLKSVFSFDY
                     KFAKVVIFSFKDSFSLKNSKRFRP"
ORIGIN      
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc acgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctctcc ttgaacagcg cgtgaagcgt
      121 caactactgg cggaagtgca gtccatctgt ccgccgggcg tgaccattat gaacgtgcgt
      181 cagggcgaac ctttaggttt gggccactcc attttgtgtg cacgacctgc cattggtgac
      241 aacccatttg tcgtggtgct gccagacgtt gtgatcgatg acgccagcgc cgacccgctg
      301 cgctacaatc ttgctgccat gattgcgcgc ttcaacgaaa cgggccgcag ccaggtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactccgtca ttcagaccaa agaaccgctg
      421 gatcgtgaag gtaaagtcag ccgcattgtt gaatttatcg aaaaaccgga tcagccgcag
      481 acgctggact cagatattat ggccgttggt cgctatgtgc tttctgccga tatttggccg
      541 gaacttgaac gtactcagcc tggtgcatgg gggcgtattc agctgactga tgccattgcc
      601 gaactggcga aaaaacagtc cgttgatgca atgctgatga ctggtgacag ctacgactgc
      661 ggtaaaaaaa tgggctatat gcaggcgttt gtgaagtatg gactgcgcaa cctgaaagaa
      721 ggggcgaagt tccgtaaagg tattgaaaag ctgttaagcg aataatgaaa atctgaccga
      781 atgtaacggt tgataagaaa attataacgg cggtgaagat tcgtggcgaa agtaatttgt
      841 tgcgaatatt cctgccgtta ttttatataa acaatcagaa taacaacgag ttagcaatag
      901 gattttagtc aaagttttcc aggattttcc ttgtttccgt agctgattgg taagacaatt
      961 agtgtttgaa attttcaggt ttagcgcgag tgggtaacgc tcgtcacatc gtaggcatgc
     1021 atgcagtgct ctggtagctg taaagccagg ggcggtagcg tgtctagggc agggaaagat
     1081 cacgtgatgc tgcttttttg tactaaacaa tttgcatttt atgttcaata attgagacat
     1141 tccttattac ctaaaactgt ttttcattgc ttatacatga gcaaatactc cttacataat
     1201 taaggagaac aaaattgaac ttaaaaaatt gatggaacat atttctatta tcctcgatta
     1261 tagacaagct aggaaagtga aacataaatt gtttgatatt ctactgttga ctatttgcgc
     1321 acttatttct ggtgcagaag gctgaaaaga tatagatggt ttttgggaaa cgcatcttga
     1381 tttttttaac aatatcgtga ttttgaaaat ggtattccta ttcactatac cattgccaga
     1441 gttgtatcct gtatcagttc tacaaaattt catgagtgtt ttattaactg gatgcgtgat
     1501 tgtcattctt aagatgataa agacgtcatt gcaattgatg gaaaaacgct ccggcattct
     1561 tatgacaaga gtcgtcgcag gagagcgatt catgtcattg gtgcattctc aactatgcac
     1621 agtctggtca tcggacagat caagacggat gaacagaaac tacctagcgt cagtccttgc
     1681 ggggagcggg ctttcgtaat cttgcctgta gcgttatttg ttttcgagaa taaatatata
     1741 atactccatg tggatattta gttaaaagag gatgtggtga aaacagattt gactgtattt
     1801 aagaatatta tatcgctatt tagcattcgt tttgctggct acataatacc tttaatcacg
     1861 ttgccgtatc ttgtcaaggt acttcaacct gttggatttg gatatttatc atttggtatt
     1921 gctgttttac aatattgtac gctagttgtt aattatgggt ttgatctttc agcaacaaga
     1981 aaaattgcac agaatagaga aaatgtgcat tacatcagcg atattttttg gaatattctt
     2041 tttttgcggt tgtttatttc tcttgtaggt tttttagtat tagtggtttt tatccattta
     2101 tttcctgatt tgtatcgaat taaatggatt ttgctttggg ggtatttgtc tgttttgggg
     2161 acagctctct ttccaatgtg gttatttcag gggaaagaac agcttggacg aatatcagca
     2221 gctcgtatat ttttacagat ttcttgcgta cctttatttt ttgtttttgt aaaaaacact
     2281 caagatgatt gggtcgcatc actaatatat gctctaccgc agttgttaat atcagtagtg
     2341 gcagtatata tcattcataa aaggaagtgg attgtattaa aaaaaataaa acttgcaaca
     2401 atcaagaaag aattaataga tggttggcat ctttttatat ctacggcagc aattagtctc
     2461 tatacaacaa ccattactgt agtcttaggg attgtaccag gtccctatag tgttgccata
     2521 tatacttctg caaataaaat attacaggcg gctcaggggc tttatcaacc aatatcctct
     2581 gcattttacc cgcgaataaa taacctgata aaagaaaata aacaattagc atttaaaact
     2641 ataagaaaac tatttaaaat acagtttgtt attactattt gcataagcgt tgggttgttt
     2701 ttgttttcta atattgttgt tgatatttta tacggtgatg attatcgaaa tgcggcatta
     2761 attcttcagc tattatcacc tattcctata gttgtgggat gtagtaacat ttttggaata
     2821 caaatattat taacatgtgg ttataaaaag gagttttcca ctattttact gatctcaggg
     2881 cttattaata tgattttgat atttcctctt tgctataagt ttaacgagtt tggagcagca
     2941 ttttctgttt taattacaga gatatttgtt actgtttcta tgctgtttgt ggttattctg
     3001 aaaaaaatac cggtttggga gagtaagcgt aatgtatgat tatttgattg tcggcagcgg
     3061 gctttttggc gcggtttgtg caaatgagct taaaaaaaga aatgtcaaaa ttcttgtgat
     3121 agataaacgc aaccatgtag gagggaatgt ttataccgaa aacattaatg gaattcaagt
     3181 gcataaatat ggtgcacata tatttcatac taatgataag tatatttggg agtatgtaaa
     3241 taaacttgtt gagtttaatc gttttacaaa ttcacctcta gcttatttta aaggggaact
     3301 ttttaattta ccatttaaca tgaatacttt ttatcagttg tggggagtga aaaaaccttc
     3361 agaagctgcc gctattatag aagaacaacg aaaagtaatg ctaaaaaaag aacctacaaa
     3421 tttggaagag caggctattt cactagttgg agtagatatc tatgagaaat tgattaaggg
     3481 atataccgaa aaacagtggg gacgtagttg taaagagtta ccatccttca ttattcgacg
     3541 actaccagta agatttacat ttgacaataa ttatttttct gacaaatatc agggtattcc
     3601 tgttggggga tatacgaatt taattgaaaa aatgcttgag ggagttgagg ttcatttagg
     3661 cattgactat cttaaagaac gcaagaaata ttcgaaaatt gcaaaaaaaa tcatatatac
     3721 aggaccgatt gatgagtatt ttgataatgt gctaggacag ttggagtata ggtcattaaa
     3781 atttcaaaca gaaaaaatgg atattcctaa ttatcagggg aatgcagtgg ttaattacac
     3841 tgaaaaagaa ataccattta cgcgaattat agaacataag cattttgatt ttgttgaatc
     3901 taattttacc ataattacta aagaataccc taaagagtgg agtaatggtg atgagccata
     3961 ctaccctatc aatgatgaga aaaacatggc tttatataga aaatataaat ctttggctga
     4021 tgaggaaaat gtgatttttg gtgggcgttt ggctgaatat aaatattatg acatgcacca
     4081 agttattcgg tctgccttaa actgtgtgaa gcatatttaa ttattaagga ggggggaggg
     4141 aggcatattt ttatgaagct atattatatc tgttcggatt tttatccttc tggcactggt
     4201 tttgcaatat cgtttacgaa ttttatacag tttatattag attcaaataa attcgagcat
     4261 atcacgatac taactacaaa ttctaaagca tctttgcctg aaaatttcaa cgaacgtgtt
     4321 ttaatgcaaa cttttgatgt cagaggtgag ttgaagttag ctaggtactt tagatcatct
     4381 attcttcttg acttgtattc aatatttaaa tatagacgca ttctgcatat gttaaaatgt
     4441 tgttgtatta agtctgatga tgtattcttt tatgaggagt tttattttgg caatctttat
     4501 aaatatttga aaagacgctt tccttgtaat aagcatataa tacgagtgca tgggacaatg
     4561 ccagagtttg tgacgtttga tcgttcaaat agatatagaa aaatattatt taaacaagct
     4621 ttagagctaa aaaacaatag agaaaataaa ataacaattg caacgacaac atctttttat
     4681 attgatttca ttaatactca tatacttaaa aatcagtatg gaacaatatg taatgtagat
     4741 tatatttttc taccaaatag tatatatagt aataaaaata ttccgcagac acaaaagtca
     4801 gaaaatatgt ccaattcttt aactttattg cagttgggga gaatggataa aggtgggtac
     4861 ttccaaaaag ggtttgatga tacgattaaa gcattaaatt atattaattc agatgttttt
     4921 ttatcccatc ggatacgtct tgttactatt ggtagtggtg agaaaaaaaa atattttaaa
     4981 gataaaatga gggatctaaa aaacatagca tttgaacatt atgaaaatat aaataatgaa
     5041 gctgttaatg acttaataat gcaggctgat gttatactct taccatcaag atgtgaaggt
     5101 atgtcaatgt ttgcagtcga agctatttcg ctaggaaaac caataattac gacaagaaat
     5161 actggagttg atgatatatg tattgaagga gttaatagtc tgaaatttga tatgtttaat
     5221 tatgttgagt atgcacaagc tatcgaaaaa ataatcaaag aaccgcattt gattagacaa
     5281 atgggatata atgcttttgc tgtttcaaaa gaaaatgaaa agaaattaaa agcaaatata
     5341 gaggcctttt tgtgatgatt tatagatata tattaaatct atttcctgac actctattta
     5401 ttagattgcg atattttaaa cgatttaagc gtattttaca cttgagaaaa ccgaagcgat
     5461 ttactgagaa aattatgtgg tataaacttt tttatcatga taatttgatg actatctgcg
     5521 cagataagta tcgtgtaaga gaatatataa aggaaaaggg gttcggtgat atattggttc
     5581 cattgtttaa agtatataat actccagatg aaataaaaat tgatgaattg ccttgtcaat
     5641 ttattttaaa agggaataat ggaagtgaga ctaatttagt tgttagggat aagagtaaaa
     5701 taaccgagaa gtatataagg catatagttt ctggatggat ggctaataac caagttatta
     5761 ctttggggaa agagtggtgt tatcagaata ttgaatttaa gataactgtg gagcagctat
     5821 tagaatcaga aggagaggat ggcattgatg actataaatt tctttgtttt aatggtcgcg
     5881 ttcattatat atgggttgat aaaaatcgat ttacaagcca caccagaact ttttttgatc
     5941 gagactggaa tgtgataaat gtagaatctg atcatccagc tgctagtttt tctatagata
     6001 agccttgggg gtttgaaaga atgctacata tcgctgaaga tataggtaaa gatttcccat
     6061 ttgctagagt tgatttttat agtgtaaaca aaaaggttta ttttggtgag ataacttttt
     6121 atccatggag tggcatggtg cagtttcaac cagatagttt tgattttgat atgggtgata
     6181 aatttattct gccaaaagag atgttataat gagtaagttg tccgtaatta tgtctgtata
     6241 ttcagagtcc gaaaaaatgc taagacaatc tattgattct gtattgaatc agtcatttag
     6301 ggattttgaa tttatcatag ttaatgataa cccagagaga atcgagataa tacagttgct
     6361 tcgttattat atggaaaaag ataatagggt taaggtattt tcaaatgaga tcaataaagg
     6421 gttgacaaag agtcttaata ttgcagcaaa aaaaagcaac agtaaatata ttgccagaat
     6481 ggacgctgat gatatttctg atattacgag gtttgataga cagttagata tacttgagaa
     6541 aaatgatgat atagatatat gtggtagtaa tgttaaggta attaatgtcc atgataatgt
     6601 tacagggcac tataggtatc ctgaaagatc ttcaaaaata aaggggaagt tattctttcg
     6661 tgattgtctt tgccatccag ccacaatgtt tcggcgttcg tttttcattg aggtcggcgg
     6721 atatagtgag gattttaaaa aagctcaaga ctatgaactt tggattaagg ttttaataaa
     6781 tgataaaggg atatataata ttcagactcc cttattatac tatcgtatgc attctgaaaa
     6841 tatttcgaca aaaaatattg atgaacaaca ttattattca ataaaggcaa aagtactact
     6901 gtattcattt tttatggatc agcagcaatc attgaaaatc atagattata tagagggaca
     6961 gcaaggcaat gttcatatta tacgtgtgtt aaagttcttt tttgttttag ctgggcgatt
     7021 aaataaaaaa attaatggtt tttcatatat atatttcttc tttttattgg tgaaaaggtt
     7081 gatttacata aaaattataa gacgtgctat tttttagtag agttaaaatc cttttattat
     7141 atcctccttg caatgagtta ttaaatgaat aacactttac gacagtatag tttagtgcaa
     7201 gtctttataa caatgtactg cctctttccc ttctattttc ataacatata tattattcag
     7261 actttttctt tatattcatt agcactaagc tttgtagttt atacgttttt ctataaagtt
     7321 aggtttgttt caaggtcagg ggcaattaca atccttgcta gcacgacaac atttttgatt
     7381 tttttattta tgagcatgct cattaatttg tcgagcgata tgacctttgt ttatgaacaa
     7441 gtttttggtt tttctaagca gttactatta gcattagttc ctttttgtct ctatttttcg
     7501 ttcgatagtg atgatattaa ttacgcagta cacagtattt tattgttgtt tattagagcc
     7561 ataagcttgt atgtttttat ttctgttata tttctgatac ctgaagttag aaattattgg
     7621 attgatatta tatattttag tagtgatcaa aataaggaat taataaattc tgcttcatac
     7681 tatacgcgct ttggtttgca gggattttct ggattcagac atgctattct atgcgcgatt
     7741 gctctaattt ttagtatata tttatttatg tgtggttata aaaaaataaa aaattattgg
     7801 atgagtgtgt ttcttgtttg tattgggtgt ttcctatacg gccgtattgc tatagctgtg
     7861 atactatgta taactttaat atcattattt tatttattat gtaagtttga aaaactttat
     7921 tttatattta ttgtttgctt atgcgtgggg acgattgcat gtgtagtata tatgaatatt
     7981 gattatattc aggatagtta ttcattagct tggattttcg aaccattaat aaattttatt
     8041 aatgaaggtc agtttacaag tcattcgaca aatgatttat ccagcatgta tataatgccg
     8101 aatgaaaata caatgttgtt aggtgatggg aaatatacta atcatgatgg aagttattat
     8161 atgcatacgg atgttggatt tttaaggcct gttttttttg gaggtatttt ttttgttttc
     8221 ttttactata tgatgttaat attgccactt atattaatta ttaagaattc tgggcttgaa
     8281 cgaagaaata tattaacatt gtttttggct ttggctttga ttgtttttga gttgaaggga
     8341 gagtcattta tatcattttc tgcaataatt ttttctttca tactcattcc tattatttgt
     8401 aaaaggttaa attgatgaga atatcattag aagagaattc agtagttttt attctatatc
     8461 aatttcatat tggcggtgtc gaaagtgcac taataaatct gcttgagaat attaaaaaga
     8521 agagagtata tttgatcttt gcaatgaata agatcaacaa tgaactccta aaaaaaattc
     8581 ctcataacgt caaaataatt ggactaggaa taaataataa aaatatatgt catttaattg
     8641 gttatacacg tagattaagc aaacgatatg attttagtag aacacacatt gttaattttt
     8701 ctgataccct atcaactcta ataattcaat attctttgaa tgcaaaatta aagacatcgt
     8761 gggttcattg caatccatta actttgataa actctcgttc atttcagtta tataagtatt
     8821 tattagttag aaatgataaa atagtttgtt tgtgtgaatc acagaaaaat ctcttaataa
     8881 aggtgatgcc caaagcggaa aaaaaagtca ccattatata taattgtgtt gatgctgata
     8941 tcctttcaaa aggagctcaa gaaaaagttg acgttgatta taaatatata ataaaagttg
     9001 caagatttga tgaaagaaca aaagatttca ttactcttat agatgcatat tgtaaactac
     9061 ctaaagaaac acaagatacg tataaacttg tattattagg tgatggacct gatctcgaaa
     9121 atgttagaaa ttatacaaga aaaaaaaaca acatgcaaaa catatttttt ccgggtaaag
     9181 atgtcaatcc ttttaaatgg attaaacatt cgcagttctt ggtgcactcc tcaacatcag
     9241 agggcttttc tcttgttctg ttggaggcca tgttattagg taaagttgtg attgcatctg
     9301 attgtaattg tggtccttct gatatactgg ataatgggaa tgcaggattc ctattcaatg
     9361 ttggtgatgt tgatagctta acctgtatta tgcaaaattt attagttaat caagacatca
     9421 tcaatatata taccaatcgc tctcttgtta gaggcgttga attacagttg aaagcaaata
     9481 ggcaggttaa ggagtatttt ttgtgagtag tgtatctgta tgcattgtga cttttaatcg
     9541 taaaaatgac ctgttgagat gtctagataa tgttcttaaa cagtcacacg ttgtcagtaa
     9601 tatccttata tatgataatg catctactga tgggaccact cggtttctgc ttagtaaata
     9661 ttgcgctata ttatctccat caaatattcc tcaaaagatt gcaaccctaa atgacataaa
     9721 tatctggctt gttacagcga cagagaactc tggaggtgct ggcgggtttc attattctgt
     9781 aatattatct cgtgaaatat tcgatactga ttattattgg ttaatggatg acgatggtta
     9841 cccttctaaa gattgtttaa atattcttat gcaatatgca gagacgcata attctgatta
     9901 cgttatgcct gttagtatag atattgataa taatgaacag ctatcttggc caacacgaaa
     9961 aaaaaaccat gcaaaaactg aatgttataa ggaattatat gagtcgtggg gagatgttat
    10021 ggattatgtt acacctttta atggttctct cttaagtaaa aaatgtattg atagtgttgg
    10081 gtatgtgaat aaagattttt ttatttgggg ggatgagtat gaacattact ggagatgcaa
    10141 aaaaagcaga attcatcctg taacaattat gaatgcaaaa ttctatcacc cgtcacaaaa
    10201 gttacctctt cagccatgtt tttttggatt gattaaatta ccctatgtcg attctccatt
    10261 acgtatggta tgcctagttc gaaattatac atatatttat aaaaaatatg actcaaaatt
    10321 aaaaataatt ctcaagctta tatcttatag ttggtttttc ttaataacaa gaaagcttga
    10381 tgctaaaggt tttgtcttat acttaaaaag tgttcttgat ggattaagaa atgattttac
    10441 aagacataga gattttttat aatacaggtt gatacatttc ataaaaacat aatgataatg
    10501 gataatcaat ttgcatctta attattttta ctaccttccc tgctcgataa atatgtttat
    10561 agggttaata tgactgttat tgctatagtt gtaacattta atcgctgtgc gcttttgaaa
    10621 aaagttttgc attcgttgct ttctcaatca attgcattaa ataaaataat aatcattgat
    10681 aatgatagta atgatgatac agctaaagtc gtgcatgatt tttctgaagt ggatgatatt
    10741 ttttattatt ataacacagg agataatctg ggaggggctg gagggtttta tcaggggttt
    10801 aaaatcgcag agcagcttta ttatgactat ctttggttaa tggatgatga ccttctacct
    10861 gagcctgatt gcctagagaa attaatccaa gataggccag aaggaattgt tcagccggta
    10921 agatacgatc ttgatggtgc ttgtgctgaa atatcacctg ttgagtataa tcttcaaaaa
    10981 atattttgca ggaatcctaa aaccaaaaca gtaaaagaag tgatttcaac agttatttct
    11041 gataattgtc gggaaatcga tattgcaggt gttccttttg aaggtccttt aatttcaaag
    11101 tcggtagtta ataaagtggg gtatccaaat cctgattttt ttatttttaa tgatgatctt
    11161 gattattcgt taaggacgag aagtaaaggt ttctctataa agtgtatagt cgacgccagg
    11221 gctacaaggc ttttaaaaaa taatcaaaaa aatgatttga aaagctggaa aggatatttc
    11281 atgttgagaa atcattatta tatcctacga aactatggtg aaaataagct cgttaagaat
    11341 cgagtctatc tcatcatgtt ttattatttt ttgaagtcag ttttttcttt tgattataag
    11401 ttcgcaaaag tggttatttt ttcttttaaa gattcatttt cgcttaaaaa tagcaaaaga
    11461 tttcgaccat agtttttatt tttggataaa cgctgttatc acaactgatc ggagtaattc
    11521 ttaaatgtca aagcaacaga tcggcgtcgt cggtatggca gtgatggggc gcaaccttgc
    11581 gctcaacatc gaaagccgtg gttataccgt ctctattttc aaccgttccc gtgaaaagac
    11641 ggaagaagtg attgccgaaa atccaggcaa gaaactggtt ccttactata cggtgaaaga
    11701 gtttgttgaa tctctggaaa cgcctcgtcg catcctgtta atggtgaaag caggtgcagg
    11761 cacggatgct gctattgatt ccctcaagcc atacctcgat aaaggtgaca tcatcattga
    11821 tggtggtaat accttcttcc aggacaccat tcgtcgtaac cgtgagcttt ctgcagaagg
    11881 ctttaacttc atcggtaccg gtgtttccgg tggtgaagag ggcgcgctga aaggtccttc
    11941 cattatgcct ggtggccaga aagaagccta tgaactggtt gctccggtcc tgaccaaaat
    12001 cgccgcagta gctgaagacg gtgagccatg cgttacctat attggtgccg atggcgcagg
    12061 tcactatgtg aagatggttc ataacggtat tgaatacggt gatatgcagc tgattgctga
    12121 agcctattct ctgcttaaag gtggcctgaa cctcaccaac gaagaattgg cgcagacctt
    12181 taccgagtgg aataacggtg aactgagcag ctacctgatc gacatcacca aagatatctt
    12241 caccaaaaaa gatgaagacg gtaactacct ggttgatgtg atcctggatg aagctgctaa
    12301 caaaggtacc ggtaaatgga ccagccagag cgcgctggat ctcggcgaac cgctgtcgct
    12361 gattaccgag tctgtgtttg cacgttatat ctcttctctg aaagatcagc gtgttgccgc
    12421 atctaaagtt ctctctggcc cgcaagcgca gccagctggc gacaaaggtg agttcatcga
    12481 aaaagttcgt cgtgctctgt atctgggcaa aatcgtttct tacgcgcagg gcttctctca
    12541 gctgcgtgct gcgtctgaag agtacaactg gaatctgaac tacggcgaaa tcgcgaagat
    12601 tttccgtgct ggctgcatca tccgtgcgca gttcctgcag aaaatcaccg atgcatatac
    12661 agaaaatccg cagatcgcta acctgctgct ggctccgtac ttcaagcaaa tcgccgatga
    12721 ctaccagcag gcgctgcgtg atgtcgttgc ttatgcggta cagaacggta tcccggttcc
    12781 gaccttcgcc gctgcggttg cctattatga cagctaccgt gccgctgttc tgcctgcgaa
    12841 cttgatccag gcacagcgtg acta
//

</pre>