<pre>LOCUS       GU068045               11292 bp    DNA     linear   BCT 25-SEP-2010
DEFINITION  Escherichia coli strain E78634 serogroup O165 O antigen gene
            cluster, complete sequence.
ACCESSION   GU068045
VERSION     GU068045.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11292)
  AUTHORS   Wang,Q., Ruan,X., Wei,D., Hu,Z., Wu,L., Yu,T., Feng,L. and Wang,L.
  TITLE     Development of a serogroup-specific multiplex PCR assay to detect a
            set of Escherichia coli serogroups based on the identification of
            their O-antigen gene clusters
  JOURNAL   Mol. Cell. Probes 24 (5), 286-290 (2010)
   PUBMED   20561581
REFERENCE   2  (bases 1 to 11292)
  AUTHORS   Wang,Q., Ruan,X., Wei,D., Hu,Z., Wu,L., Yu,T., Feng,L. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2009) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P.R. China
FEATURES             Location/Qualifiers
     source          1..11292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E78634"
                     /db_xref="taxon:562"
                     /note="serogroup: O165"
     misc_feature    108..11174
                     /note="O antigen gene cluster"
     gene            108..1106
                     /gene="wcnP"
     CDS             108..1106
                     /gene="wcnP"
                     /note="epimerase or dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcnP"
                     /protein_id="ADN43876.1"
                     /translation="MFDNKTILVTGGTGSFGNKFVRMTLEKYNPKKIIIYSRDEMKQW
                     EMAKHFKDESRIRFFIGDVRDKDRLYRALDGVDFVVHAAATKIVPTAEYNPFECVKTN
                     INGAINVIDACIDKGIERVVALSTDKASSPANLYGATKLASDKLFVAGNSYSGATKTR
                     FAVVRYGNVMGSRGSVIPFFLSIKEKGELPITDNRMTRFMITLEQGVELVWHAFEDMV
                     GGEIYVKKIPSMKVTDIATAVAPNAIQKEVGIRPGEKLHEQMISAEDAYYTYEYPEHF
                     KILPAIHNWCNSPERIKDGKKVPEGFVYESDTNTEWMSIEELCQWIEDNREKVGNI"
     gene            1106..2260
                     /gene="wcnQ"
     CDS             1106..2260
                     /gene="wcnQ"
                     /note="aminotransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcnQ"
                     /protein_id="ADN43877.1"
                     /translation="MKFIPYGRQDISGEDISAVVDVLKSDFLTQGPYVPKFEKAIADY
                     VNAKYAVAVNSATSALHIACLALGMKKGDWLWTSPNTFVASANCALYCGANVSFVDID
                     ARTYNMSVSALEAKLMTAKNNGTLPKVVVPVAFAGQSCEMEAIYKLSKEYGFTIIEDA
                     SHAIGGSYQGEKIGSSHFADITILSFHPVKIITTAEGGMALTNNDSLAEKMQLFRSHG
                     ITRDINHMTKVSEGDWYYQQIDLGLNYRMTELQAALGMSQLKRIDGFVTRRHELAERY
                     KKSLEGIPISLPFQAEGGYSAFHLYPITVKNSAQRKSLFDYLRNKSIGVNVHYIPVHT
                     QPYYEKLGHKVGDYPIAEDYYSRAISIPMYSALTNEAQDYVIQCIQEFFL"
     gene            2257..2967
                     /gene="wcnR"
     CDS             2257..2967
                     /gene="wcnR"
                     /note="cytidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcnR"
                     /protein_id="ADN43878.1"
                     /translation="MNVAIIPARGGSKRIPRKNIKMFCGKPMIAWSIDAAKKSGVFDR
                     IIVSTDDEEIAAVARKFGAEVPFMRPDALSNDFMGTIPVIRHAAEWLASNGNVANFIC
                     CIYATAPFICPKDIIIGLDILRKEQADYAFTVTRFPYPIQRSLKINKEKKISMFFPDM
                     YHVRSQDLEESWHDAGQFYWGTSSAWLNETPIFSANSCSIELPRERVQDIDTPEDWIM
                     AEWLYKTMEFKNESVSAG"
     gene            2945..4483
                     /gene="wcnS"
     CDS             2945..4483
                     /gene="wcnS"
                     /note="acetyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcnS"
                     /protein_id="ADN43879.1"
                     /translation="MKVFLRVDSSLSIGSGHIIRCINIATMLRKMGAECIFITKNHKG
                     NIISKIRQADFLFEVMSVLEEPEYYISNEKEWLNGRQSDDAVQFSSLLSQYSQKPDII
                     IVDHYSLDSEWEIIVKQNFPQARLVVIDDLCNRKHHCDLLIDQTYLRHKHEYAELILP
                     ETKILTGTKFALVNTDFYKFRNESIIRKKNLKRPLKLMITMGGVDANNITGKVLQYLE
                     EINYKNIQKITVIIGSKCPHRTEICELTRNSKYNIQVVIDISNMAELMLEHDFAIGAL
                     GGTTWERCVMGLPAVNIAIARNQHTVAQNLSKSGAIVLYSDKFKKSEFSNALDNLMAN
                     YYQQSNLARIICDGHGLYRIVQEIIPNYAKDGINVALRPATIDDISFVYKLQCEPHTR
                     RFARNPNIPTFENHSVWMRCKLLEEKSLFYIIEHEGACGVLRLDAIEHKTAQYEISVF
                     LTSRCIGKGIASAAIRRAMMLNNNSIIMATVLLENYASHQLFKNLGYKQISQDKYISR
                     KNDE"
     gene            4473..5537
                     /gene="wcnT"
     CDS             4473..5537
                     /gene="wcnT"
                     /note="CMP-N-acetylneuraminic acid synthetase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcnT"
                     /protein_id="ADN43880.1"
                     /translation="MMNEFITINGRKIGNGYPPYIIAELSANHNGDLERAFKIMEEAK
                     LSGADAIKLQTYRADTITIDCDSEDFQIHGGLWGGQTLFNLYKNAQMPWEWHQPLFEK
                     ARKLGITIFSSPFDFTAVDLLEDLGAPAYKIASFEAIDIPLIRYVASTKKPMIISTGM
                     ADEQEILEAINAAKEGGCKELVVLHCVSGYPAPAEDYNLATIPDIAKRFGVITGLSDH
                     TIDNTTAIVSIALGANVIEKHVTLDRNGGGPDDSFSLEPAELAGLCRDVQTAWKALGA
                     VNYERKESEKGNVKFRRSLYAVKRIDKGELFTNDNVKSIRPGFGLPPVYLDCILGKRA
                     SQNIEYGTPMKMEFVSGYRE"
     gene            5534..6784
                     /gene="wzx"
     CDS             5534..6784
                     /gene="wzx"
                     /note="O antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ADN43881.1"
                     /translation="MSFQNIVDFSFRALTLGGRFVLLIAIAKYLSPDDVGIYGILCSL
                     ISYVLYFLGIDFYVYSTRKIICGGVNKIWGMLYNQYVFYLFGYVLLIIVSQQVFTYSG
                     ISDYAVMGVAIALFEHMSQEMYRVLIVIKKIRAANIQLFIRSGSWCYITSLLLCIKNI
                     TDLKSVMFIWMLFSFISVCYALFVIYVEVHPKREDFILDLSWIIEGVKVSSFFFIGTI
                     CLRGIFYFDKIFLKDIVNLNIVGVYVFYFGVASAIQSFVDILVVSKYYPELIRQVNED
                     IDRGNVSLSKKIVREFLNKILATAFFLCIVSIPACYFVVKFLNKSDYLAYFPVYISLL
                     VANVVFLIAMPYHYFLYALKRDSFLVRINIFTFGLFLLLSYVLIRLFSDLTVYCIIVP
                     IIVSYSSALFIKFYGYNSFVRGRS"
     gene            6781..8214
                     /gene="wcnU"
     CDS             6781..8214
                     /gene="wcnU"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcnU"
                     /protein_id="ADN43882.1"
                     /translation="MNNVLVLINNAIFAKFFISLSANLKMLGYEVTIICDSSYTYEKF
                     DIESLGIESIIFTDYFINKADSDELLQKQNLKDQWVSHADYDRMKYFGFSYKNKPHFL
                     NYYNAALFSFFTELFDSKQYHAIIYENVSNALSYAAYQVGKTKGVKYLGLTASRFPGH
                     ALFSIMDDILAEEILAVGNRCYDEITAEDELFLNSYMDNIINIVPDYMKTNGMSKPVF
                     LSKLLKMRNIKTVMAAIKHSLMKSSNYDIQNGKPLIRSFNAHKRNLMRLLRSKWIKQY
                     YTNNFNGKKYIVYPLHYHPESSTSILAHYHDEFNLIKNIAFSLPTGYSLLVKDHMSAY
                     GFNSLAFYRDISSLPGVYIISPEKNAKELVIDSIGVITLTSTVGYEAAILDKPVVVFG
                     DVFYEKHKRVLKLSNYDQISNAIDYIINSSSESKNTDASLDNKQFLYGYYKKCFPFDM
                     NYTSNKEKREEDARYISIMIDKELRSE"
     gene            8207..9544
                     /gene="wzy"
     CDS             8207..9544
                     /gene="wzy"
                     /note="O antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ADN43883.1"
                     /translation="MNKNSNFFLFLLVIDVFFTIILCCASYYFHENYTLRCITLFLFI
                     ALLLIILKINFLFFYLSLLLIYGYSGQILSLFFIEQGGSYLNELQNQSHSNGAMSLLI
                     LIVTFAIMINYIFFTLVFRNLTKVRLWFANGNNTVRLINVLFIFAYISIIFLYGNAYG
                     SAGGNRVIYRHDYLPSWAGIFIDLQWIPVLVLGLLYINNKKKEFFIFLILHLFFRVLF
                     GEKFTMLVQLLYISFIPYVIFFHQEINVNRFIKIVLFFGVLLGTSIFLNYFFIENAGF
                     ERIEQRIAQQGQLWWYVVNNYLEKGGSISVINEFFSYNKGDCQQGMDLLMCKSMSPSL
                     YQLYIEKQYVLTSGYPSILLLYFGPYGIILFLFASPLFLLPLVMLYVSLKNKKILASI
                     ISFRVFFSFFSFYAIGNMHDLLNLKTLIYFLLWLGVYSIPKIVFVNRKACNYD"
     gene            9537..10421
                     /gene="wcnV"
     CDS             9537..10421
                     /gene="wcnV"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcnV"
                     /protein_id="ADN43884.1"
                     /translation="MTKLAIGILTYKRMDYLIETLKDINKTKYEIELIILNNNEGYCV
                     KNLILPLINNKKIQVRYLWDKTNYGVAKGRRKIVENCSSEFLIIFDDDIFIRDINVVI
                     CNVVTEFENNPALGGIAFNILNYQTKKHCRYEIPHKNKKINLDLEFDTYLFIGAGMAL
                     RISSVLKVGNFSRDLGPYGFEEVDLAFRLINAGYVIKYNPKCIIEHKKSPDGRFSHEM
                     VNYYAFVNRTIIAKKHLLTRYYISCFIIRSLFFLYKTGNFKLLIKGWKEIYKMKSKNS
                     FSKNFYIYCKKVSAFLYY"
     gene            10431..11174
                     /gene="wcnW"
     CDS             10431..11174
                     /gene="wcnW"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WcnW"
                     /protein_id="ADN43885.1"
                     /translation="MIMPLITVVMPAYNSSLFIEQAILSVLKQTYTKFELIVCDDSST
                     DNTMDIIERFSQLDPRVRVIKNKFFKGAAGARNSCLEMAKGEYICFLDSDDYWCENKL
                     EIQLKFMLENNLDFCHTDYCMFIEDRAKVIKARNVINFDSLTYTCDIGCLTVMLRKSI
                     IGVTRFPNLPKEDYAFWLLILKQGITAKKLNEVTAYYRKHKSSLSSNKIKEIYRQYYV
                     LSKVTKYNLAKILIRLFAYICVALAKHKM"
ORIGIN      
        1 attggtagct gtaagccaag ggcggtagcg tatgttaaac ttaaaggagt tgttgcgagc
       61 aatatagatc gtggcaattt cgtatagtgg cttattttgg tgaaactatg tttgataata
      121 aaacgatttt ggtaactggt ggtactggtt catttggtaa taaattcgtt cgaatgacat
      181 tagagaagta taatcctaaa aaaattatta tttattcccg tgatgaaatg aaacagtggg
      241 aaatggcaaa acatttcaag gatgaaagca gaattcgatt ttttattggt gatgttcgcg
      301 ataaagatcg tttgtatcga gcgctggatg gtgttgactt cgttgtccac gctgcagcaa
      361 caaaaattgt gcctacagca gagtacaatc ccttcgaatg tgtaaaaaca aatatcaatg
      421 gtgcgattaa cgtgattgat gcttgtattg acaaaggtat cgaacgtgta gtcgctcttt
      481 ctactgataa agcaagcagt cctgctaatc tgtatggtgc aactaagttg gcatccgata
      541 aactttttgt tgcgggtaat tcttattctg gggcgacaaa aactcgtttt gctgttgttc
      601 gttacggaaa tgtaatggga tctcggggct cagtaatacc gttcttctta tctatcaaag
      661 aaaaaggtga attgcccatt acagataatc gtatgacccg ctttatgatt actctggagc
      721 agggagttga gttagtttgg catgcgtttg aagatatggt tggtggagaa atttatgtaa
      781 aaaaaatacc ctctatgaaa gttacagata tagctactgc tgttgcgcct aatgctatac
      841 aaaaagaagt cggtattcga ccgggtgaga aactacacga gcagatgatc agtgctgaag
      901 acgcttatta cacctatgaa tatccagaac attttaagat tcttccggcg attcataatt
      961 ggtgtaactc acctgaaaga attaaagatg gaaaaaaagt gccagaaggt ttcgtttatg
     1021 agagtgacac taacacagaa tggatgagta ttgaagagct gtgtcaatgg atcgaagata
     1081 accgagaaaa agtaggtaat atctaatgaa atttattcca tatggacggc aggatatctc
     1141 aggcgaagat attagtgctg tagtggacgt actgaaatca gattttttga ctcaagggcc
     1201 atatgttcct aaatttgaaa aagcaatagc tgactatgtg aatgctaaat atgcagttgc
     1261 agtaaatagt gcgacttcag cacttcacat tgcctgtctt gcattgggga tgaaaaaagg
     1321 cgattggctt tggacatcac caaatacttt tgttgcttct gcgaactgtg ctctttattg
     1381 tggcgcaaat gtcagttttg ttgatattga tgcgcggaca tataatatga gcgttagtgc
     1441 cttagaagcg aaactcatga ctgcgaaaaa taatggaact ttacctaaag ttgttgttcc
     1501 tgtcgcattt gcaggacagt catgtgaaat ggaggcaata tataaactct caaaagagta
     1561 tggatttaca attatcgaag atgcttcaca tgcaattggc ggaagttatc agggagaaaa
     1621 aataggtagt tcgcattttg cagatattac tatattgagc tttcatcctg ttaaaattat
     1681 cacaactgca gaagggggga tggctctaac caataacgat agtttggcgg aaaaaatgca
     1741 gctattccgt agccatggaa ttactcgtga tattaatcat atgacaaaag taagtgaagg
     1801 cgattggtat tatcaacaaa ttgatcttgg actgaattac cgaatgactg aacttcaggc
     1861 agcgttgggg atgagtcagt tgaaacgtat tgatggcttt gtaacacgcc gtcatgaatt
     1921 ggctgaacgt tacaaaaaat ctcttgaagg tattcctatt tctctgccat tccaggccga
     1981 aggtggctac agtgcttttc acctttatcc tataactgtt aaaaattctg cgcagcgaaa
     2041 atctttattt gattatctgc gaaataaaag catcggggtt aatgttcact atatcccagt
     2101 tcatactcaa ccgtattatg aaaagctagg gcataaagtc ggtgattatc ctatagctga
     2161 ggattattat tcccgagcaa ttagcatacc gatgtactct gctctgacaa acgaagctca
     2221 ggattatgtt atccaatgta ttcaggagtt ttttttatga atgtggcaat cattcctgct
     2281 cgtggtggta gcaaacgtat cccgcgtaaa aatattaaaa tgttttgcgg gaaaccaatg
     2341 attgcttggt cgattgatgc cgctaagaag agcggcgtgt ttgacagaat cattgtttcg
     2401 acggatgatg aagaaattgc ggcggttgcg agaaaattcg gcgcagaagt tccgtttatg
     2461 cgacctgatg ctctatcaaa tgattttatg ggcacgattc ctgtaatacg acatgctgct
     2521 gagtggcttg ctagcaatgg aaacgtggcg aattttattt gttgtattta tgccactgca
     2581 ccttttattt gtcctaaaga tattattatt ggattagata ttcttaggaa agaacaggcc
     2641 gattacgctt ttactgttac tagatttcct tacccaattc aacgttctct taagataaat
     2701 aaagaaaaaa aaattagtat gttctttcca gatatgtatc atgtccgatc tcaagatctt
     2761 gaagagtctt ggcatgatgc tgggcaattt tattggggaa cgtcatctgc atggttaaat
     2821 gaaacaccaa tatttagcgc taattcttgt tcaattgaac taccgcgtga gcgtgtacag
     2881 gatatcgata ctcctgagga ttggatcatg gctgaatggt tatataaaac catggagttt
     2941 aaaaatgaaa gtgtttctgc gggttgattc gtcgctatct ataggttctg gtcatattat
     3001 tcgatgtata aatattgcaa caatgttaag aaagatgggg gcggaatgta tatttataac
     3061 taaaaatcac aagggaaata ttatttctaa aattagacaa gctgactttt tgtttgaagt
     3121 tatgtctgtt ttagaagaac cagaatatta tattagcaat gaaaaagaat ggctgaatgg
     3181 tagacaaagt gatgatgcag tgcaattcag ttcattgctt agtcaatatt ctcaaaagcc
     3241 cgatatcatt attgttgatc attattcact tgacagtgaa tgggagataa ttgttaaaca
     3301 aaattttcca caagcaagat tagttgttat tgacgatctt tgtaatcgaa aacaccactg
     3361 tgatctattg atagaccaga catatttacg tcataagcat gaatatgctg agttaatttt
     3421 acctgaaact aaaattttga cagggactaa atttgcgcta gtaaatacgg acttttataa
     3481 atttagaaat gaatctataa tacgcaaaaa aaacttgaaa cgccccctca aactaatgat
     3541 aacaatgggg ggggtagatg caaataacat aaccggtaaa gtactacagt atctcgaaga
     3601 aataaattat aaaaatattc aaaaaataac ggtgataata ggtagtaaat gtcctcacag
     3661 aacagaaatc tgcgaattaa ctagaaattc aaaatataat attcaagtcg taatcgatat
     3721 ctcaaacatg gcggaactta tgttggaaca tgatttcgcc ataggtgcat tgggagggac
     3781 tacttgggag cgttgtgtaa tggggctacc agctgtaaac attgccattg ctcgtaatca
     3841 gcatacagtc gcgcagaatc tttcaaagtc aggtgcaata gttttatatt cagacaaatt
     3901 taaaaagagt gagtttagta atgcattaga taacttgatg gcaaattact atcagcaaag
     3961 taaccttgca agaataattt gcgatggaca tggattgtat agaattgtac aggaaattat
     4021 tcctaattat gcaaaggatg gtataaatgt tgcgcttaga ccagctacaa tagatgacat
     4081 tagttttgta tataaacttc agtgcgagcc ccatacgcga cgatttgcac ggaatccaaa
     4141 cattcctacg tttgagaacc attcggtttg gatgcggtgt aaattgttgg aagaaaaatc
     4201 tttattttat attatagaac atgagggggc atgtggtgtt ttacgactgg atgcaattga
     4261 acacaaaaca gcgcaatacg aaatatctgt atttttaaca agtcgttgca ttggaaaagg
     4321 tatagcctct gccgccatta gacgagcaat gatgttgaat aataattcca ttataatggc
     4381 tacagtttta ttagaaaatt atgcttcaca tcagcttttt aagaatttag gctataaaca
     4441 aatatctcaa gataaatata ttagtaggaa aaatgatgaa tgaatttatt acaataaatg
     4501 gtcgtaaaat tggtaatgga tatcctccat atatcattgc tgaattatca gcaaatcata
     4561 atggtgatct agaacgcgca ttcaagatta tggaagaagc aaaactttcg ggagctgatg
     4621 cgattaagct acaaacttat cgtgctgata ccattactat agattgtgat tctgaagatt
     4681 ttcaaattca tggaggatta tggggggggc aaacgctatt taatttatac aaaaatgcac
     4741 aaatgccttg ggaatggcat cagccattat tcgaaaaagc gagaaagcta ggaattacca
     4801 ttttcagcag tccatttgat tttactgcag tggatttgct ggaggatctt ggcgcaccag
     4861 catataaaat tgcgtctttt gaagcaattg atattccgtt aattaggtat gttgccagta
     4921 caaaaaaacc aatgattatc tcaactggta tggctgatga gcaggaaatc cttgaagcaa
     4981 ttaatgcagc gaaggaaggt ggttgtaaag aattagtcgt tttacattgt gtaagtgggt
     5041 atcctgctcc agcagaagat tataatcttg caacaattcc tgatatagcg aagcgttttg
     5101 gtgtgataac tggtttgtca gatcatacaa ttgacaatac tacagcgatt gtatcaattg
     5161 cattaggagc taatgtaatt gaaaagcatg tcacactaga tagaaatgga ggggggccag
     5221 acgatagttt ttcccttgaa cctgctgagt tagctggatt atgtcgtgac gtacaaactg
     5281 catggaaagc acttggtgct gtgaattacg aaagaaaaga aagtgaaaaa ggcaatgtaa
     5341 aatttagacg ttcattgtat gctgtaaaga gaatcgataa aggtgaatta tttactaatg
     5401 acaatgtcaa aagtattcga cctggattcg gccttccccc agtttatctc gattgtatat
     5461 tagggaaaag agcttcacaa aatatagaat acggaactcc tatgaaaatg gaatttgtat
     5521 caggttacag agaatgagct ttcagaatat tgttgatttc tcatttagag ccctcacttt
     5581 aggagggcgt tttgtgctat taatagccat agcaaaatat ttatctccag atgatgttgg
     5641 aatatatggt atattgtgta gtctaatttc atacgtacta tattttttag ggattgattt
     5701 ttatgtttat tccacacgaa aaattatatg tggaggcgta aataaaatat gggggatgct
     5761 atataaccaa tatgtgtttt atttattcgg gtatgtactc ctaattatag tatcacaaca
     5821 ggtatttaca tattctggca tttcagatta tgctgttatg ggagttgcga ttgctttatt
     5881 tgagcatatg tctcaagaga tgtatcgtgt attaattgta ataaaaaaaa ttagagcggc
     5941 aaatatacaa ctgtttatcc gaagtggtag ttggtgctat ataacttcat tactgttatg
     6001 tattaaaaac attacggatc ttaaaagtgt aatgtttata tggatgctgt tttcgtttat
     6061 ctcagtttgt tatgcacttt ttgtaatata cgttgaggtg catccaaaaa gagaagattt
     6121 cattttagat ttatcttgga taatagaggg ggttaaggtt tcctcatttt tttttattgg
     6181 cacaatatgc cttcgaggga ttttttactt tgacaaaata ttcttgaaag atattgttaa
     6241 tttaaatatt gttggtgttt atgtgtttta ttttggtgtt gcatctgcaa ttcaatcatt
     6301 tgttgatata ttagttgtat caaaatatta tccggagtta atcagacaag ttaatgaaga
     6361 tattgataga ggcaatgtat cgctatcaaa aaaaattgtg cgggaatttt taaataaaat
     6421 actcgccaca gcattttttt tgtgtattgt ttcaataccc gcctgttatt ttgttgtaaa
     6481 attcttaaat aaatctgatt atcttgctta ctttcctgtg tatattagcc ttttggtggc
     6541 aaacgtcgtt tttttgatcg cgatgccata tcattatttt ctgtatgcgt taaagcgtga
     6601 ttctttctta gtaaggataa atatttttac gttcggttta tttttactat tgagctatgt
     6661 tttgatacgg cttttttctg atttaactgt ttattgcatc atagtcccta ttattgtttc
     6721 atactcaagt gctttattta ttaaatttta tggctacaat tcgtttgtta gagggcgctc
     6781 gtgaataatg ttctagtact tattaataat gcaatatttg ctaaattctt tatatcactt
     6841 tcggctaacc ttaaaatgtt aggatatgag gttacaataa tatgtgatag ctcttataca
     6901 tatgaaaagt ttgatataga aagcttgggg attgaatcta taatatttac tgattatttt
     6961 attaacaaag ctgatagcga tgagttattg caaaaacaaa atttgaaaga tcagtgggta
     7021 tcacacgctg attatgatag aatgaaatat ttcggattta gttataaaaa taaacctcat
     7081 tttttaaatt attataatgc tgctcttttt tcttttttta ctgagctatt tgactccaag
     7141 caatatcatg caattatata tgaaaatgta tcaaatgctt tatcctatgc tgcatatcaa
     7201 gttgggaaaa ctaaaggtgt taaatattta gggttaactg ctagccgttt tcccggtcat
     7261 gccttattta gtattatgga tgatatactg gcagaagaaa tcttagccgt tggtaatcgt
     7321 tgttatgatg aaataacagc tgaagatgag ttgtttctaa attcttatat ggataatata
     7381 attaatatag tgcctgatta tatgaaaacg aatggtatgt caaagcccgt atttttaagc
     7441 aagttgttaa aaatgcgcaa tataaaaaca gtcatggcgg ctataaaaca tagtcttatg
     7501 aaaagttcta attacgatat tcaaaatggt aaaccattaa tacgttcatt caatgcacat
     7561 aaacgtaatt taatgagact gctgcgttct aaatggatta agcaatatta tacaaataac
     7621 tttaatggta aaaaatatat tgtctatcct ttgcattatc atcctgaatc atcaacatca
     7681 atattagctc attatcatga tgagtttaat ttgataaaaa atatagcatt ttcattacct
     7741 acaggttatt cattgttagt gaaggatcac atgtctgcgt atggatttaa ttcgttagca
     7801 ttttatagag atatatcttc cttaccgggg gtttatatca tatcacctga gaaaaatgca
     7861 aaagagttgg tgattgactc gattggagtt ataacgttaa ctagtactgt tggatatgaa
     7921 gctgcaatat tagacaaacc tgtagttgta tttggtgacg tattttatga aaaacataaa
     7981 agagttctta aattgagtaa ctatgatcag attagtaatg ctatagatta tattatcaac
     8041 tcatcatctg agtcaaaaaa tactgatgcc agccttgata ataagcaatt tttatatggc
     8101 tattataaga aatgttttcc atttgatatg aactatacgt caaataaaga aaaaagagaa
     8161 gaggatgccc gatatatttc tattatgatt gataaggagt taagaagtga ataaaaactc
     8221 aaacttcttt ctttttttgt tagttataga tgtctttttt acaattatat tgtgttgtgc
     8281 atcatattat ttccatgaaa attatactct tagatgtata actctgtttt tatttatagc
     8341 gttattattg attattttaa aaataaattt cctttttttt tacttatccc tgctgttaat
     8401 ttatggttat tcagggcaga tattgtcatt attttttata gagcaaggtg gtagttatct
     8461 aaatgaactg caaaatcagt cacattcaaa tggagcaatg tcattattaa ttttaatagt
     8521 gacatttgca ataatgatca actatatttt ttttacatta gtgtttcgta atttgactaa
     8581 ggttagactt tggtttgcta atggtaataa tactgttagg ttgattaatg tattatttat
     8641 ttttgcatat atctcaataa tatttttgta tggcaatgca tatggttcag ctggcggtaa
     8701 tagagtaata tatcgtcacg actacctgcc atcatgggct ggcattttta ttgatttaca
     8761 atggatccct gttttggttc tggggttact atatataaat aataaaaaga aagagttttt
     8821 catatttctt atactacatt tgttctttag agtacttttc ggtgaaaaat tcacaatgct
     8881 tgtgcaattg ttatatattt catttattcc atatgtgatt ttttttcacc aagaaataaa
     8941 tgttaatcgt tttatcaaaa ttgtattgtt ttttggtgtt ctgctgggga cctcaatttt
     9001 tctgaactat ttttttatag aaaatgccgg ttttgaaaga atcgaacaaa gaatagcgca
     9061 gcaggggcaa ttatggtggt atgtagttaa taattatttg gaaaaaggag ggagcatttc
     9121 agtgattaac gagtttttct catacaataa aggggactgt cagcaaggta tggatcttct
     9181 tatgtgcaag agcatgtccc catcgttgta ccagctatat atagaaaaac aatatgttct
     9241 aacatcaggt tatccctcta ttttgttatt atattttgga ccctatggta ttattttgtt
     9301 tttattcgca tccccacttt ttttattgcc attggtaatg ttatatgtat cacttaaaaa
     9361 taaaaaaata ctggcttcaa ttatatcatt cagagttttt ttctcattct tttcatttta
     9421 tgctataggt aatatgcatg atttgttaaa cctgaaaaca cttatttatt tcttgttgtg
     9481 gttaggggtt tattcaatac caaaaattgt ttttgttaat aggaaagctt gtaattatga
     9541 ctaagttggc aataggtatt ttaacatata aaagaatgga ctatcttata gaaaccttga
     9601 aggatataaa taaaaccaag tatgagattg aacttattat cttaaataat aatgaaggat
     9661 actgcgttaa aaatctaata ttgccgctta ttaataataa aaaaatacaa gtgcgttatc
     9721 tttgggataa gacaaattat ggtgttgcaa aaggtcgacg gaagattgtt gaaaattgtt
     9781 cttctgagtt cttgattatt tttgatgatg atatatttat acgcgatatt aatgttgtta
     9841 tttgtaatgt agtgaccgag tttgagaaca atccagcgct gggaggaatt gctttcaata
     9901 ttttaaatta ccaaacaaag aaacactgca gatacgaaat tccacataaa aataaaaaga
     9961 taaatctaga cttagaattt gatacttatt tgtttatcgg ggctggaatg gctcttagaa
    10021 tatcaagtgt tcttaaagta ggtaactttt cgagagattt gggaccttat ggttttgaag
    10081 aggttgactt agcttttaga ttaataaatg caggatatgt tattaaatat aatcccaaat
    10141 gtattattga gcataaaaaa tctccagatg gtagattttc ccatgaaatg gtaaattact
    10201 atgcatttgt aaatagaaca attattgcta aaaaacatct tctcacaagg tattatattt
    10261 cttgttttat tatccgttct ttatttttct tatataaaac aggtaatttt aaattgctaa
    10321 taaaagggtg gaaggaaata tataaaatga agtccaaaaa ttcattttca aaaaattttt
    10381 atatatattg taaaaaagta tccgctttct tatattacta aatatatcat atgatcatgc
    10441 ctcttataac tgtagttatg cctgcttata actcatcatt attcatcgag caagcaattt
    10501 tatcagtatt aaaacaaaca tatactaaat tcgaactgat tgtatgtgat gattcctcca
    10561 cagataatac tatggatatt atcgagagat tttctcaact tgacccccgt gtacgtgtaa
    10621 ttaaaaataa attttttaaa ggtgctgcag gggcacgaaa ttcttgcttg gaaatggcaa
    10681 aaggagaata tatttgtttt ttagatagtg atgactattg gtgtgagaat aaactagaaa
    10741 tacaattaaa atttatgcta gaaaataatt tagatttttg ccatactgat tattgtatgt
    10801 tcatagagga tagggctaag gttataaagg caagaaacgt tattaacttt gattctttaa
    10861 cttatacttg tgatattggt tgtcttacag taatgttgag aaaaagtata attggagtta
    10921 caagatttcc taatcttcca aaagaggatt atgccttctg gctgctgatc ttaaagcaag
    10981 ggattactgc aaaaaaactt aatgaggtga ctgcatatta tagaaagcat aaatctagtt
    11041 tatctagtaa taaaattaaa gagatttatc ggcaatatta tgttttatca aaagtgacga
    11101 agtataactt agccaagata ttaattcgac tctttgcata tatatgtgtg gcactagcta
    11161 aacataaaat gtgaataatt tatttatact gaacaataaa cttggacttt taccacacta
    11221 atcaagttat gcttccgcct gaaaggagta aacaatgcca agcaacagat cggcgtcgtc
    11281 ggtatggcag tg
//

</pre>