<pre>LOCUS       KP710593               13121 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain SN3B-1 serotype O163:K-:H19 O antigen gene
            cluster, complete sequence.
ACCESSION   KP710593
VERSION     KP710593.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13121)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13121)
  AUTHORS   Baranzoni,G.M., Yan,X., Fratamico,P.M., Debroy,C., Tebbs,R.S.,
            Liu,Y., O'Connell,C.D., Swimley,M. and Matheny,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2015) Molecular Characterization of Foodborne
            Pathogens, United States Department of Agriculture, 600 E Mermaid
            LANE, WYNDMOOR, PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SN3B-1"
                     /serotype="O163:K-:H19"
                     /db_xref="taxon:562"
     misc_feature    381..12879
                     /note="O antigen gene cluster"
     gene            381..1820
                     /gene="manC"
     CDS             381..1820
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase 1"
                     /protein_id="AJR19402.1"
                     /translation="MMNHEKIFPIIMAGGAGSRLWPLSRELYPKQFIRLKGELTMLQA
                     TLSRLNTLMCNDPIIICNEQHRFIVAEQVRQLNKRTENIILEPVGRNTAPAVALAALA
                     AKRSRPDCDPLMLVLAADHVIQNEDAFREAVKVAIPYAENGKLVTFGIVPDTPETGYG
                     YIRRGEALFSVDEFKAFAVKTFIEKPNLETAIDYVSSGEYYWNSGMFMFRAGRYLEEL
                     EKYRPDILSACEKSMAVVDLDLDFIRVDEEAFLCCPEESIDYAVMEHTADAVVVPMDA
                     GWSDVGSWSSLWEVSDKTREGNVCTGDVISLNSGNNLIFSDTSLIATVGVHNLIIVQT
                     KDAVLIADRAAVQDVKKVVEKIKTDGRHEHHIHREVYRPWGKYDSIDTGERYQVKRIT
                     VNPGEGMSVQMHHHRAEHWVVVAGTAKVTINGEAKLLGENESIYIPLGATHCLENPGK
                     IPLDLIEVRSGSYLEEDDIIRFQDRYGRV"
     gene            1927..3327
                     /gene="algC"
     CDS             1927..3327
                     /gene="algC"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="AJR19403.1"
                     /translation="MEKLTCFKAYDVRGKLGEELNEDIAWRIGRAYGEYFKPRTIVLG
                     GDVRLSSKALKLALAKGLQDAGVDVLDIGLSGTEEIYFATFHLGVDGGIEVTASHNPV
                     DYNGMKIVREGARPISGDTGLRDVQRLAEANDFPAVNEAKRGSYKQINLQKEYIDHLL
                     GYVNVANFKPLTLVINSGNGAAGPVVDALEARFKALNVPIKFVKVNNTPDGNFPNGIP
                     NPLLPECRSDTSNAVIELGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KHPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVTEMLCLKGKSLGEMVRDRMTAYPASGEINSTLAHPAEAIAR
                     VEQHFAKEALEVDRTDGLSMSFDEWRFNLRSSNTEPVVRLNVESRSNIELMEIKTKEI
                     MALLDNNNSGGRAENV"
     gene            3320..4573
                     /gene="wzx"
     CDS             3320..4573
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigene flippase"
                     /protein_id="AJR19404.1"
                     /translation="MFSQLIQQLSSFVARILVSLISLLLSVIIVRLYTKDIVGDFFLL
                     TSYSTVLAQVFLFGMAPVLNIMSSKGVQLNKIVIEIAKKNVISMLCMFLCGLIILAFF
                     AKVDYSSLIVLSLLSGVLLIISELMKGQGMYILSQIFNGGLNNILFLILIVVFIPNKE
                     ETDLEDIIYFYALAYMCVIVGGSIYLRIKYKVSKSKGLIDDVITYSIILPIFISTVIV
                     YMFSQIDLWFVSRLFDNDVVAQYGLAIRLTAVLSFSTLSVRAIAAARIPKLMNDIQAL
                     QKDVSLSCLFSFLVSLVILSGIALFGKLFIGLVYGNSYELTWYVLLIFSFGQMINAAT
                     GPCDYLLSHTGHGRYLMIISLVSFLFLMTLLAIITIRFNSVFLFCGAVSFTICLQNLI
                     IMYVAFKKTGVLSLPLINFKRAYVS"
     gene            4570..5805
                     /gene="wzy"
     CDS             4570..5805
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AJR19405.1"
                     /translation="MTRKMNTAVLSFMMLYILTSLISVYQTLNQDLFPGELHRYVRTI
                     ADDEVVIITTLNIISFIICYITFLLFQKFRFRLNKNYHYELNDKRIHFIIFVVLISQI
                     IFLYYTGIGKVTVNVEQRSTSSFSAFFAILKPEPFIFFYFLYYRLKPGFTFIKNKIFI
                     FNMFLFIVFKLFQGWTSFLLILFFLEVHARVQFTKNRLRYVLFIPLIIIFFGGWVYQY
                     AYVLKNEIRGAKVESITYLQGVEHLASRLSMNPNSLGAYQNYSKVINLYQQEGLALKE
                     SKAFLRPITPGGLVDKNFRNINNNVMTSYTPDLNQFTSSDFGIVMYYSILFNASLPDF
                     VLSIIMTVLLLVIAKMFFDSISSIPGQCDILFFFVLFYTFYTVSLENVFGQNFIPYIF
                     SFVIYYFLGGIKKVHFVEN"
     gene            5817..6830
                     /gene="gumI"
     CDS             5817..6830
                     /gene="gumI"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose:glycolipid
                     4-beta-D-mannosyltransferase precursor"
                     /protein_id="AJR19406.1"
                     /translation="MIMIVKMWPLQKESTANQYTSLILHDVIEYKPHKWNLKNVLDMR
                     FDIFHMHWPETYLNLPKRYQQLIGTLCVVLFLVVCKLFSKKIVWTVHNFKPHENHNNI
                     KISEFFRTFLVRMVDKFIFLTEVSKDEFLKIYNIDNSRLTVIHHPLYQVNECCHDISA
                     ANHSSINNKYLFFGLIRPYKNIELLMNEFIKYDSDSVLIIMGGCSNTLATELRNMKKK
                     IDFKERIKFKFGYYNEQQLQQELNQCKGVIIPYSDIMNSGVLYRAITAGVNVLLPRIR
                     YTEEVVNSLKYQGAVFYDPPLDTNDIATFNGIPSVNHNNFDVDTYNTYIKNAHYELYQ
                     TII"
     CDS             6850..7953
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferases group 1"
                     /protein_id="AJR19407.1"
                     /translation="MKNILIVCDYFYPAFKAGGPIKSLGNIAKTFNDRNYITVLTYNQ
                     DIDGQIINKSGTEIFEKNIVVYYAQNLLNFVKLYLKLYKTADIIYLNSFFSSRTTFPA
                     LMLKKTSAKVLLAPRGELTLGALTLKPLKKKIYIKLFNIFANKNEIYFQFTSEEEKNE
                     SLISLKKGFSYTVIPNMHDPIPPYIYKHKKEGEIHISFISRISPKKNLKAVLLSLLNI
                     AQGDILLSIAGNIEDEKYWAECMLIINKLPQNVKVNYLGGIKPTQVIDLLKSSHLFFL
                     PTLNENYGHAIVESMINSNVVLISDQTPWSDVQWNGGYVAGYNDTDTFKKCITECMKF
                     DESQFNNKSRQVYDYARNALTKHEYKTSTLFDI"
     gene            8138..9169
                     /gene="fnlA"
     CDS             8138..9169
                     /gene="fnlA"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="AJR19408.1"
                     /translation="MFKDKVLLITGGTGSFGNAVLKRFLDTNIKEIRIFSRDEKKQDD
                     MRKKYSNNKLKFYIGDVRDYSSVLNASRGVDFIYHAAALKQVPSCEFYPMEAVKTNII
                     GTDNVLEAAIANKVSRVVCLSTDKAVYPINAMGTSKAMMEKVIVAKSRNVPDEMVICA
                     TRYGNVMASRGSVIPLFVNQILTGKPITITDPNMTRFMMTLDDAVDLVVHAFKHGQNG
                     DIFVQKSPAATIEVLTHAILEIMKSADHTVNIIGTRHGEKLYEVLCSREEMLVAEDQG
                     NYYRIPCDKRDLNYEKYFEKGNKKVETIDDYNSHNTYRLNTQEMVSLLRKLTYINKIE
                     AGEKADPDE"
     gene            9162..10037
                     /gene="rmlD"
     CDS             9162..10037
                     /gene="rmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="AJR19409.1"
                     /translation="MNKRILIVGANGMLGSSLLRYFTQYSRYDVLGTVRSNSAKAKLS
                     KQGFDNIISQVDVLEYETIENVISDWKPDFLFNCVGIIKQLDAAKNNILSLSINSLLP
                     HQLAKTCTRNETKLIHFSTDCVFSGKQGNYCEESFPDAYDLYGKSKQLGEVVYDGHLT
                     LRTSIIGHEISSQHSLIDWFLSQKKYIYGYSKAIFSGMPTVYVAEIIHDYILPNPNCC
                     GLRHLSVDPIDKFSLLKLVKKQYSHNIEIIESSDLEIDRSLDSFLLRQEVGFYPASWP
                     KLIEKMNDEYNKFFR"
     gene            10009..11166
                     /gene="fnlC"
     CDS             10009..11166
                     /gene="fnlC"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacetamido-2,3-dideoxy-D-glucuronate
                     2-epimerase"
                     /protein_id="AJR19410.1"
                     /translation="MMNTINSSVKKMKVVTVVGTRPEIIRLSRVMAVLDQYTEHTIIH
                     TGQNYDYELNEVFFKELEIRKPDYFLNAAGSNPAETIGNIIIEADKIFDIISPEALLI
                     LGDTNSALVSIAAKRRKIPIFHMEAGNRCFDYRVPEEINRKIVDHIADINLTYSEIAR
                     EYLLREGIPADQIIKTGSPMREVLNYYNGEINKSKALEKLNLTPNEYFLVSAHREENV
                     DSPEKLKIFINILNKISDKYDLPIIVSTHPRTRNRMDKLNVEISNNVYFMKPFGFLDY
                     VSLQKNARVVLSDSGTITEESSILNFPALNLREVHERPEGFEEAAVMFVGLDAVRIFQ
                     GIEILDQQQRGPDNRDLYLVNDYSVDNVSLKVLRIIMSYTNFVNQKVWKKQ"
     CDS             11166..12359
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="AJR19411.1"
                     /translation="MRIALICDDYLPDSTRVSAKMMHELACGLLREGHEPVVFCPNGG
                     VGTELKVIYLDGICIYKYPNGQTKDISKIKRAFNETMLSFNAWHYLSSEIKRKKIDGV
                     IYYSPSIFFGAFVKKIKKYWKCKSYLILRDSFPQWLVDQRIIRRNGMLEKYFRFFEKK
                     NYTAANYIGVMSSKNKELFTLQYPHYKNVDVLYNWADLNSTQDVSPSDILERLSLADK
                     VIFFYGGNIGHAQDMANLMRLAIGMRTINYVHFLFVGQGDEVELVKKTIAENALENCT
                     YLPAISQSEYKSILKVVHVGLFSLAKNHSVHNFPGKLLGYMANKLPILGSVNEGNDLM
                     QVLIGAKAGYVHVNGYDAELLQSAIKLATEEKLRNELGENGHSLLEKTFSTQSAVENI
                     LLRLK"
     gene            12382..12879
                     /gene="wbuC"
     CDS             12382..12879
                     /gene="wbuC"
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC"
                     /protein_id="AJR19412.1"
                     /translation="MSVTVFYKEQLNELSNLASNNPRLRAHLNLHKSYQDKIQRILIS
                     LKRGTYIPPHYHKYDHQWEHFQVIEGIVDLYIFNNSGTVLDIINLGDVNGALFAQIEP
                     YTIHTLVCRSPYASVIEIKEGPFVEAEAKIVPSWVYPEGCREYLRSDIVSMLETLKIN
                     EVYKL"
ORIGIN      
        1 tctggtagct gtaaagccag gggcggtagc gtgtctgatg aataaatcga tttgttcaat
       61 atagtaatct actagaaaaa cttttcatcc ctcataatta tcgatgtcaa tatttgaaat
      121 ataattagca accaacagtt gattaaaata gttcaatgtt tctgcataat aattatattg
      181 atggtaaaaa attaaacata acgaacccta ttgttttatg cgtttattta taacgtaagg
      241 cccatttaat agctctgtaa ataatgtaat tcacttaaat tttctttgtt ttaccaaata
      301 ccctttggtt taaccgaatt ttatatttaa ccttatcttc aaggtatgag ttatagctct
      361 gggccttaga atgagttaat atgatgaatc acgaaaaaat tttcccaata atcatggctg
      421 gtggggcagg tagtcgtctg tggccactgt cccgcgagct ttatccaaaa caattcattc
      481 gccttaaagg cgaactcacc atgttacagg caactctaag ccgtttgaat actttgatgt
      541 gtaatgatcc gattattata tgcaacgaac agcaccgctt tattgttgca gagcaggtgc
      601 gccagctgaa caaacggacc gaaaatatca ttttggagcc tgtcggtcgt aacaccgctc
      661 cggcagttgc cctcgcggcg ctggcggcaa aacgcagtcg cccggactgt gatccgttga
      721 tgctggttct ggctgctgac catgttattc agaacgaaga tgcatttcgc gaagcggtaa
      781 aggtggctat cccttacgcg gaaaacggca agttggtgac tttcggcatc gtgcctgata
      841 cgccggaaac gggatatggc tacattcgtc gtggtgaggc tctttttagc gtagatgagt
      901 tcaaagcctt tgctgtaaaa acatttatag aaaagcctaa tcttgaaact gcgatcgatt
      961 acgtctcaag tggtgagtat tactggaaca gtggtatgtt catgttccgt gcaggtcgct
     1021 acctggaaga acttgaaaaa taccgtccgg atatcttaag tgcctgcgaa aaatcgatgg
     1081 cagtggttga tcttgatctc gattttatcc gcgtggatga agaagccttt ctctgctgtc
     1141 cggaagagtc cattgactat gcagtgatgg aacatacggc ggatgccgtg gtggtgccaa
     1201 tggatgcggg ctggagtgat gtcggttcat ggtcttcttt atgggaagtt agcgataaaa
     1261 ctcgtgaggg aaatgtttgc actggagatg tgatttcatt gaactctggc aataatctta
     1321 ttttctccga tacaagctta attgccacag ttggtgtgca taatctaatt atagtacaga
     1381 ctaaagacgc ggtactgatt gctgaccgtg ccgctgtgca agacgttaaa aaagtggtgg
     1441 agaagatcaa gactgatggt cgccatgaac accatattca tcgtgaagtc taccgtccat
     1501 ggggtaaata tgactccatc gacaccggtg agcgttacca ggtgaaacgc atcacagtga
     1561 atccgggtga aggaatgtcg gtgcagatgc accatcaccg tgccgaacac tgggtcgtgg
     1621 tggctggtac cgccaaagtg accatcaatg gtgaagcgaa gctgctcggt gaaaacgagt
     1681 ccatttatat tccgctgggt gcgacgcatt gcctggaaaa cccggggaaa attccgctcg
     1741 acttaattga ggtgcgctct ggctcctatc tggaagagga cgacatcatc cgcttccagg
     1801 atcgctacgg gcgggtgtag cactccgcat catgcccagt ggcgctgagc ttgctggatc
     1861 tacgaatgaa aaaatagatt tgaccattca agggtcagga ctaattttgc ctaaaaagga
     1921 gccatcatgg aaaaattaac ctgttttaaa gcttacgatg ttcgcggcaa gctaggtgag
     1981 gagctgaatg aagacattgc gtggcgcatc ggccgcgcgt atggcgaata ttttaagcca
     2041 cgaactatcg tcttaggcgg cgatgtgcgt ctgtccagca aagccctgaa gctggcgttg
     2101 gcgaaaggac tgcaggacgc tggtgttgat gtgcttgata tcggcctttc cggtaccgaa
     2161 gagatctatt ttgcaacttt ccatttgggc gtggacggtg gtattgaagt gacagctagc
     2221 cataacccgg tggactataa cggtatgaag attgtgcgcg aaggtgcacg tcctatcagc
     2281 ggtgataccg gcctgcgcga tgtgcagcgc ttggctgagg ccaacgactt cccggcggtg
     2341 aatgaagcca aacgcggtag ttataagcaa atcaacttgc aaaaagagta catcgaccac
     2401 ttgctaggct atgtcaacgt ggcaaacttc aaaccactca ctcttgtaat caattccgga
     2461 aatggagcgg caggaccggt tgtcgatgca cttgaagctc gctttaaggc gctgaacgtg
     2521 cctataaaat tcgttaaagt gaacaatacg cctgacggca acttcccgaa cggtattcct
     2581 aacccgttgt tgccagagtg ccgttctgat acaagcaatg cggtgattga gctcggtgca
     2641 gacatgggaa ttgcctttga cggtgatttc gaccgctgct tcctgttcga cgaaaaagga
     2701 cagtttatcg agggttacta tatagttggt ctgctggcgg aagcctttct tgaaaaacac
     2761 cctggtgcaa aaattatcca tgatccgcgt ctctcctgga acactgttga tgtggtgact
     2821 gccgcaggtg gcacgccggt aatgtcgaaa acaggacacg cctttattaa agaacgtatg
     2881 cgcaaggaag acgccatcta cggtggcgaa atgagcgcgc accattactt ccgtgatttc
     2941 gcttactgtg atagcggcat gatcccatgg ttgctggtga cggagatgct gtgtctgaag
     3001 ggcaagtctc tgggcgagat ggttcgcgac cgcatgactg cctatcctgc aagcggtgaa
     3061 atcaacagca ccttggcaca ccccgctgag gcgatagcgc gggtggagca gcactttgcg
     3121 aaagaggcac tggaggtgga tcgcaccgat ggtcttagca tgtcgtttga tgaatggcgc
     3181 ttcaatctgc gttcgtcgaa taccgagccg gtggtacgtt tgaacgtcga atcccggtct
     3241 aatatcgagc taatggaaat aaaaactaaa gagataatgg ctctattaga taataataat
     3301 tcagggggcc gagctgagaa tgtttagtca gctaattcag cagttgagct cgtttgtagc
     3361 gaggatatta gtatcattaa tttctcttct tctcagcgtg ataattgtac ggttatatac
     3421 gaaagatata gttggtgatt tttttttgct aaccagttat tcgactgttt tggcccaagt
     3481 gttcttgttt ggaatggcac ctgttttaaa cattatgtca tccaaggggg ttcagttaaa
     3541 taaaatagta atagagatag ccaagaaaaa tgtaatatct atgctatgta tgtttttatg
     3601 tggcttaatt atactggctt tttttgctaa agttgattat tcgagtttaa ttgtgctttc
     3661 acttctttca ggagttctat taattattag tgaactaatg aagggacaag gtatgtatat
     3721 tctctcccaa atcttcaatg gaggtttgaa taacattctc tttcttattc ttatagtcgt
     3781 ttttatacca aataaagaag aaacagatct cgaggatatt atttattttt atgcgttagc
     3841 atatatgtgt gttattgtag gtggttctat atacttgcgc attaaatata aggtaagtaa
     3901 gagtaaagga ttaattgatg atgttattac gtattcaatt attttaccga tattcatatc
     3961 aactgttatt gtatatatgt tctcacaaat agacctgtgg tttgtatctc gtttatttga
     4021 taatgatgta gttgctcagt atggattagc aattagattg acggcagtat tgagtttttc
     4081 gactttatca gttagagcta tagcagcagc aagaatccca aaattaatga acgacattca
     4141 ggcgttgcaa aaagatgtat ccctcagttg tttattttca tttttagtga gtttagtgat
     4201 tttaagtggg atagcattat ttggcaaatt atttattggc cttgtttatg gcaacagcta
     4261 tgaattaact tggtatgttc tactaatttt ctcatttgga cagatgatta atgctgcaac
     4321 tggtccctgc gattatttac taagtcatac agggcatgga agatacctta tgataatctc
     4381 acttgtttca ttcttatttt taatgactct attggcaata ataacaataa gattcaacag
     4441 tgtgttttta ttctgtggcg ctgtatcatt tacaatctgc ttgcaaaatc tgattataat
     4501 gtacgtagca tttaaaaaaa cgggtgtact ttcactaccc ttaataaatt ttaaaagagc
     4561 atatgtatca tgacaaggaa aatgaatact gcagttttat cattcatgat gttatatatt
     4621 cttactagtc ttatttcagt ttatcaaaca ttgaatcaag atttatttcc tggtgaactt
     4681 catagatatg taagaactat tgctgatgat gaggttgtga ttataacaac tctgaatatt
     4741 atatccttca taatatgtta tataacattt ttgttatttc aaaaatttag gttcaggctg
     4801 aacaaaaact atcattatga attgaatgat aagcgtatcc atttcataat tttcgtagtg
     4861 ttaatatctc aaattatttt tctctactac acaggtattg gtaaagttac tgttaatgtc
     4921 gaacagcgta gtacttcatc atttagcgct ttttttgcaa tcttgaagcc agaacctttt
     4981 atttttttct attttttata ttatagattg aaacccggat ttacttttat taaaaataag
     5041 atttttattt ttaatatgtt tttgtttatt gtttttaaat tatttcaagg gtggacaagt
     5101 tttttgttaa tacttttctt tcttgaagtt catgccagag tacaatttac taaaaacagg
     5161 ttgagatatg tactctttat acctttaatt attatttttt ttggtggctg ggtttatcag
     5221 tatgcttatg tattaaagaa tgaaattagg ggggcgaaag ttgaaagtat tacgtacttg
     5281 cagggagtgg aacatcttgc atcacgttta tcgatgaatc ctaactcgct tggggcttat
     5341 caaaattatt ctaaagttat aaacttatat cagcaagaag gtcttgctct caaagaatca
     5401 aaagcatttc ttcgacctat tacaccaggt ggtttggtgg ataaaaattt cagaaatata
     5461 aataataatg taatgacatc atatacaccg gatctaaatc aatttacgag ttcagatttt
     5521 ggcattgtaa tgtactattc aatattgttt aatgcaagtc tacctgattt tgtattaagt
     5581 ataataatga ctgttttact gttagtaatt gctaaaatgt tttttgattc aattagtagt
     5641 attcctggac agtgcgatat tttattcttt tttgtattgt tctatacctt ttatactgtt
     5701 agtttagaaa atgtttttgg gcaaaacttt attccttata tctttagttt tgtaatatat
     5761 tattttttag gtggaataaa gaaagtacac tttgtagaaa attaatttga gattgtgtga
     5821 ttatgatagt taaaatgtgg cctcttcaaa aagaatcaac ggcaaatcaa tatacttcgt
     5881 tgattctaca tgatgtgatt gaatataaac ctcacaaatg gaatctgaaa aatgtgttgg
     5941 atatgaggtt cgatattttc catatgcatt ggcctgaaac ttatttaaac ttacctaaac
     6001 ggtatcaaca gttaattggg actctgtgtg ttgtattgtt tttagttgta tgcaagcttt
     6061 tttctaaaaa aatcgtatgg accgttcata atttcaagcc tcatgaaaat cacaataata
     6121 ttaagatatc agaatttttt cgtacattcc ttgttcgaat ggtagataaa tttatttttt
     6181 taacagaagt tagcaaagat gaatttctga aaatttacaa tattgataat tcaagattaa
     6241 ctgtgataca tcacccactg tatcaagtaa atgagtgttg ccatgatatt tcagcagcta
     6301 atcattcatc aattaataat aaatatcttt ttttcggatt aattaggcct tataaaaaca
     6361 tagagttatt aatgaatgaa tttattaaat atgattctga ttcggtattg ataattatgg
     6421 gagggtgctc caacacatta gctactgaat taaggaacat gaagaaaaaa attgatttta
     6481 aagaacgcat caaatttaaa tttgggtact ataatgaaca acaacttcag caagagctca
     6541 atcaatgtaa aggtgtaata ataccatact cagacataat gaattctggt gtgctatatc
     6601 gtgcaataac tgcgggtgta aatgtgttac tgcctcgtat aagatataca gaagaagtgg
     6661 tcaattctct taaatatcaa ggtgctgtat tttatgaccc tccacttgat acgaatgata
     6721 tagcgacgtt taatggcata ccctcagtaa atcataataa ttttgatgtg gatacatata
     6781 acacatatat aaaaaatgca cattatgaac tttatcaaac cataatttaa gacttaaatt
     6841 aagcgtaaaa tgaagaatat attaatagtt tgtgattatt tttatcccgc tttcaaagcg
     6901 ggagggccta ttaaaagtct cggaaatata gccaaaactt tcaatgacag aaattacata
     6961 accgtattga cctacaatca ggatattgat ggtcagatca taaataaatc tggcactgaa
     7021 atttttgaaa aaaatattgt cgtttattat gctcaaaacc tgctgaattt tgttaaattg
     7081 tatttaaaac tttacaaaac agctgatata atctatttaa atagtttttt ttcgagtaga
     7141 actacgtttc ctgcattaat gcttaagaag actagcgcga aggttttgct ggcccctcgt
     7201 ggtgaactaa cattaggtgc attgacgtta aaaccattaa aaaagaaaat atatataaaa
     7261 ttatttaata ttttcgctaa caaaaatgaa atttattttc aatttacatc tgaagaagaa
     7321 aagaatgaat cattgatatc cttaaaaaaa ggattttcat atactgtcat tcctaatatg
     7381 catgatccga ttccacctta tatatataag cataagaagg aaggtgaaat ccatatttca
     7441 tttatttcgc gtatttcccc caaaaaaaat ctcaaagcag tgctattgtc tcttttaaat
     7501 atagcccaag gcgacatact ccttagtatt gcaggaaata tcgaggatga aaaatattgg
     7561 gcggaatgta tgttgataat aaataaatta ccacagaatg ttaaagtaaa ttatctcggt
     7621 ggaatcaagc ctactcaggt aattgattta cttaagagct cgcatttgtt tttcttgcca
     7681 acattaaatg agaactatgg gcatgctatt gttgagtcca tgataaactc aaatgttgtg
     7741 ctaatatccg atcaaacacc atggtccgat gtgcaatgga atggcggtta tgttgcaggt
     7801 tataatgata ctgatacatt caagaagtgt ataaccgaat gtatgaagtt cgatgagtcg
     7861 caatttaata ataaatcacg acaagtctat gactatgcac gaaatgcttt aactaaacat
     7921 gaatataaaa catcaacttt attcgatatt tagatgtaaa tttatgcttt ttcattttac
     7981 ttaagtttct atgtaaacag ttattcccaa ttaacggtgt agtattattt aacatctttt
     8041 atttaaaatt ttcaatatgg taatgaattg agttttattg ctcgcttttc ttcatataaa
     8101 aacactttac gtgtttgctc actctagagg aattagtatg tttaaagata aggttttact
     8161 tattacaggt ggcacaggtt catttggtaa tgctgtatta aagcgttttt tagatacgaa
     8221 tatcaaagaa attcgcattt tttcgagaga tgagaaaaaa caagatgata tgcgaaagaa
     8281 atatagtaac aataaactca agttttatat cggtgacgta agggattatt cgagtgtgtt
     8341 aaatgcatct cgaggagttg attttattta tcatgcggct gcattaaaac aggttccttc
     8401 ttgtgaattt tatccaatgg aagcagtaaa aactaatatt attggcactg ataatgttct
     8461 ggaagcagct attgccaata aagtgtcaag agtagtgtgt ttgagtactg ataaagcagt
     8521 atacccaatc aatgctatgg gaacatctaa agcaatgatg gaaaaggtca tagttgctaa
     8581 atctcgtaat gttcctgatg aaatggttat atgtgcaaca cgttatggga atgttatggc
     8641 ttcaagaggt tctgttattc ccctttttgt taatcagata ttaactggta aaccgattac
     8701 aatcaccgat ccaaatatga ctcgtttcat gatgacatta gatgatgcag ttgatttggt
     8761 tgtacatgct tttaaacacg gacaaaatgg agatattttt gtacagaaat ctcctgctgc
     8821 taccattgaa gttttgactc atgcgattct agaaattatg aaatcggctg atcacactgt
     8881 taatattatc ggtacacgtc atggcgaaaa actatatgag gttttatgta gcagagaaga
     8941 gatgttggta gccgaagatc aaggtaacta ttatagaata ccgtgcgata agcgagattt
     9001 gaattatgaa aaatattttg agaaaggcaa caaaaaagtt gaaacaatag atgattataa
     9061 ttcacataat acatatcgtt taaatactca agagatggtt tcattactac gtaaacttac
     9121 ctatatcaat aaaattgaag ctggtgaaaa ggctgatcct gatgaataaa agaatattga
     9181 ttgttggtgc taatggcatg ctgggcagca gtctgctacg atattttacc caatatagta
     9241 gatatgatgt tttgggtact gtacgtagta acagtgcaaa ggcaaaacta tctaaacaag
     9301 gttttgataa tatcatatct caagttgatg tgcttgaata tgaaacaatc gagaatgtta
     9361 tttcagactg gaaaccagat tttttattta attgtgtagg tattatcaaa cagcttgacg
     9421 ctgcgaagaa taacatactt tcattatcaa tcaattcttt gttgccacac caacttgcta
     9481 aaacatgtac tcgtaatgaa actaaactaa tacatttttc tactgattgc gtatttagtg
     9541 gtaagcaagg taattattgt gaagaaagct ttccagatgc ttacgatcta tatggaaaat
     9601 caaagcaact tggtgaagtg gtttatgacg gtcatttaac tttacggact tcaattattg
     9661 ggcatgaaat ctcaagccag cacagtttga tcgattggtt tttatctcaa aaaaaatata
     9721 tttatggata ctcaaaagct attttttctg gaatgccaac agtatatgta gccgagatta
     9781 ttcacgacta tattcttcca aatccaaact gttgtgggct aagacacctt agtgttgatc
     9841 ctattgataa attcagctta ttgaagctag taaaaaagca atactctcac aatattgaga
     9901 ttatagaatc aagtgattta gagattgatc gtagtcttga ttcatttcta ttacgccaag
     9961 aagtggggtt ttatccagca agttggccta aactgattga gaaaatgaat gatgaataca
    10021 ataaattctt ccgttaaaaa aatgaaagtt gttactgttg tgggtaccag accggagata
    10081 attcgtttat cgagagtaat ggctgttctt gatcagtaca cagaacatac catcattcac
    10141 actggacaga actatgatta tgaacttaat gaggttttct tcaaggagtt agagatcaga
    10201 aaaccagatt acttccttaa tgcagcaggt tctaacccag ctgaaactat tggtaatata
    10261 attattgaag cagacaaaat ttttgacata atttctccag aggctctttt aatacttggg
    10321 gatacaaata gtgcactagt ttctattgca gctaaaagac gtaaaattcc aatatttcat
    10381 atggaagcag gaaacagatg ttttgattat cgagttcctg aggaaattaa tcgaaagatt
    10441 gtggatcata ttgccgatat aaaccttact tacagcgaaa ttgctcgtga gtacttgttg
    10501 cgtgaaggga ttcctgcaga tcagattatc aaaacaggta gcccaatgcg tgaggtttta
    10561 aattattata atggagagat aaataaatct aaagcactcg aaaagttgaa tttaactcct
    10621 aatgagtatt ttctggtcag cgcacatcga gaagagaatg tagattctcc tgaaaaatta
    10681 aaaattttta ttaacattct taataaaata tcggataaat atgatttgcc aataatagta
    10741 tctacgcacc cacgaacccg taatcgaatg gataagctta atgtagaaat aagcaataat
    10801 gtttatttta tgaaaccatt cggtttctta gattatgttt ctttgcaaaa gaatgctcgc
    10861 gtagttctgt ctgacagcgg tacaataacg gaagaatctt cgattttaaa ttttcccgct
    10921 cttaatttac gtgaagttca tgaacgtccg gaaggtttcg aagaagctgc cgtaatgttc
    10981 gttgggctgg atgctgttcg tatttttcaa ggtattgaaa ttttggatca acagcagaga
    11041 ggaccggata atcgagattt gtatttggtc aatgattatt cggttgacaa tgtatcgcta
    11101 aaagtcctta gaatcataat gagctatact aattttgtta accaaaaggt ttggaaaaag
    11161 caataatgcg aatagcgtta atctgtgatg attatctgcc tgatagcaca cgtgttagtg
    11221 caaaaatgat gcatgaattg gcttgtggac ttttaagaga aggccatgag ccggttgtat
    11281 tttgccctaa tggtggtgtt ggtactgaat taaaagtaat ttatttggat ggaatatgta
    11341 tttataaata tccaaatgga caaacaaaag atatatcaaa aattaagcgt gcatttaacg
    11401 aaactatgct ttcattcaat gcatggcatt acctttcaag cgaaattaaa cgtaaaaaaa
    11461 ttgatggtgt tatttattat tcaccatcaa ttttctttgg ggcttttgta aaaaaaatta
    11521 aaaaatattg gaaatgtaaa tcatatctaa ttttacgtga ttcatttccc caatggttag
    11581 ttgaccaaag gataattcgt agaaatggta tgcttgagaa atattttcgt ttttttgaaa
    11641 agaagaatta tactgcagcc aattatattg gtgtaatgtc atctaaaaat aaagaacttt
    11701 ttacattaca gtatccccat tacaaaaacg tagatgtctt atataattgg gcagatctta
    11761 atagcactca agatgtttca ccaagtgata ttttagaaag gctatcattg gctgataaag
    11821 ttatattttt ctatggagga aatataggtc atgctcaaga catggcaaat ttaatgagat
    11881 tggcaatagg tatgcgtact attaattacg ttcattttct ttttgttgga caaggggatg
    11941 aggtcgaact tgttaaaaaa acaattgccg aaaatgcact tgagaactgt acatacttac
    12001 ctgcaattag tcagtcggaa tataaatcaa ttttaaaggt tgttcatgta gggttgttta
    12061 gccttgcaaa gaatcattct gtgcataatt ttccgggcaa acttctagga tatatggcaa
    12121 ataaattacc aattttaggt agtgtgaatg aaggtaatga tcttatgcaa gtgctcattg
    12181 gcgcaaaggc tggatatgtg cacgttaatg gatatgatgc agaattattg caatcagcta
    12241 taaaattagc tacggaagag aaattaagga atgaattagg agaaaatggt cattcacttt
    12301 tagagaagac cttttctacg caatctgctg tcgagaacat tttgttacgg cttaagtgaa
    12361 ttattaaatg taggaattga tatgtcggtt actgtatttt ataaagagca gttaaatgaa
    12421 ttgtcaaatc ttgcaagtaa taaccctcgg ctaagggctc atttaaactt acataagagt
    12481 tatcaagata agattcagag gattttaatt tccttaaaga ggggaacata cataccgcca
    12541 cattatcata aatatgatca tcagtgggaa cattttcaag tgatcgaggg tatagtcgat
    12601 ttatatatat ttaataattc aggtactgtc ttagatatta ttaatttggg agatgtaaac
    12661 ggtgctttat ttgcacaaat agaaccctat accatacata cacttgtctg ccgttctcct
    12721 tatgcttctg taatcgaaat taaagaaggg cctttcgttg aggcagaggc taaaattgtt
    12781 ccctcttggg tgtatccgga aggctgtaga gagtacttac gttctgatat cgtatcaatg
    12841 cttgaaactc tcaagattaa tgaagtatat aagctttaac tgattaagga taatatggaa
    12901 tcaatttaat taaatcaatc accacctccc taggatgtgg tttaggaata acaattataa
    12961 aatttcttaa tgttgaaaga ggctgcagtt tccctctatt tttttattag gacctgagtt
    13021 agcatcaaag ttacattcaa gccgcataca tcgcggtgaa caccccctga caggagtaaa
    13081 caatgtcaaa gcaacagatc ggcgtcgtcg gtatggcagt g
//

</pre>