<pre>LOCUS       GU220361               15878 bp    DNA     linear   BCT 29-JUN-2010
DEFINITION  Escherichia coli serogroup O161 O-antigen gene cluster, complete
            sequence.
ACCESSION   GU220361
VERSION     GU220361.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15878)
  AUTHORS   Li,X., Perepelov,A.V., Wang,Q., Senchenkova,S.N., Liu,B.,
            Shevelev,S.D., Guo,X., Shashkov,A.S., Chen,W., Wang,L. and
            Knirel,Y.A.
  TITLE     Structural and genetic characterization of the O-antigen of
            Escherichia coli O161 containing a derivative of a higher acidic
            diamino sugar, legionaminic acid
  JOURNAL   Carbohydr. Res. 345 (11), 1581-1587 (2010)
   PUBMED   20510395
REFERENCE   2  (bases 1 to 15878)
  AUTHORS   Li,X., Perepelov,A.V., Wang,Q., Senchenkova,S.N., Liu,B.,
            Shevelev,S.D., Guo,X., Shashkov,A.S., Feng,L., Wang,L. and
            Knirel,Y.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-NOV-2009) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P. R. China
FEATURES             Location/Qualifiers
     source          1..15878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O161"
     misc_feature    1495..13941
                     /note="O-antigen gene cluster"
     gene            1495..2685
                     /gene="lea1"
     CDS             1495..2685
                     /gene="lea1"
                     /note="C6 dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="Lea1"
                     /protein_id="ADJ19193.1"
                     /translation="MNQILELIGRESALFAQDINKYESDLSLAVSESRFLVLGGAGST
                     GQAVVKEIFKRNPRKLHVVDISENNLVELVRDIRGSFGYITGDFQTFALDIGTVEYDA
                     FINDDGQYDYVLNLSALKHVRSEKDPFTLMRMIDVNIINTDKTIQQSIDAGVKKYFCV
                     STDKAANPVNMMGASKRIMEMFLMRKSEYISISTARFANVAFSDGSLLHGFRQRLTKK
                     QPIVAPDDIKRYFLSPQESGELCLMSCIFGDNRDIFFPKLNESLHLITFADIAIKYLK
                     QCNYEPYLCNSEEEARKLVKILPNQGKWPCLLTKSDTTGEKDFEEFFTEKEKLDMNCF
                     ESIGVIKNDLLYNNKLLSSFEDEIMRMKNKKEWTKSQIVKLFLTMLPDFNHKETGKYL
                     DSKM"
     gene            2696..3838
                     /gene="lea2"
     CDS             2696..3838
                     /gene="lea2"
                     /note="aminotransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="Lea2"
                     /protein_id="ADJ19194.1"
                     /translation="METSLLIEFIRECYKTSKFIPLHAPIFSGNERTYVMETLDSTFV
                     SSVGKFVNEFERKVKEFTGSAYAVATVNGTAALHSALYMAQVKPGDFVITQALTFVAT
                     CNSIYHLGAEPIFVDISPVSLGLCPRAVERYLEENAELDETGCIHRQTGRRIKTIVPM
                     HTFGHPVELDELAAICFKWKLTLVEDAAESLGSFYKGRHTGTIGALGAISFNGNKIIT
                     TGGGGIVLCNSEDLGKRLKHLTTTAKIPHPYEFFHDEAGFNYRMPNINAALGCAQIEM
                     LNEFLLKKRKLAQMYKDFFSLSDVEFVTEPEYAHSNYWLNSIICVDQQQRNEIIEQTN
                     NSGVMTRPVWQLMHRLPMFKNSLCGELTYSEFIEARLVNLPSTPII"
     gene            3848..4840
                     /gene="lea3"
     CDS             3848..4840
                     /gene="lea3"
                     /note="putative alanyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="Lea3"
                     /protein_id="ADJ19195.1"
                     /translation="MEITTFIDLSKDIYYQQEYSALYEWDGKIFEYLYNENDYFVKFR
                     ALKRKITSVAGELINEELYDLETPYGYGGPRTNCSDDGFLARAFASYRAHCKQHNIVS
                     EFIRFHPFNKLAENSMLFDMHALERQVVIVDLTLNSEERRQLYSKKTRGIINKASKEL
                     TISIDDVELSEFTEMYYETMKKNKADAFFYFDECYFEKLMQIDGVNLIAARKDKNCAS
                     IGFFLCSNDLAHYHLSANNQRFSKENGNYLLLDAAFEYAKTKGCKYMMLGGGRTSSQE
                     DGLFKFKSKFSPATKPFYIAGLSFWPEKHQYLNQIWLEKNIKNVTPEILQLYRV"
     gene            4849..5850
                     /gene="lea4"
     CDS             4849..5850
                     /gene="lea4"
                     /note="CMP-N-acetylneuraminic acid synthetase"
                     /codon_start=1
                     /transl_table=11
                     /product="Lea4"
                     /protein_id="ADJ19196.1"
                     /translation="MGKKVFIIAEAGVNHNGDIDLAKKLVDAAVQAGVDAVKFQTWKT
                     ELLVTESAEMAEYQIENTQVKESQFQMLKRLELSYNDFVELKTYCDFKNIIFMSTPDE
                     EQSATFLDNLQSIFKIGSGELTNIPSLRHIARFSKPVILSTGMGYLSEVEHAIETLCN
                     AGLSLDMITVLHATTNYPTEPKDVNLLAMRTIAQAFPGVNVGYSDHTNGIEIPIAAVA
                     LGAKVIEKHFTLDKMMCGPDHKASLEPQELKHMVTAIRSTELALGNGWKIPTDVEMQN
                     RNIVRKSIVASKPIVAGTIITSDMLEIKRPGSGISPSRWDEVVGSVAKKDYQIGELI"
     gene            5850..7016
                     /gene="lea5"
     CDS             5850..7016
                     /gene="lea5"
                     /note="CMP-N-acetylneuraminic acid synthetase"
                     /codon_start=1
                     /transl_table=11
                     /product="Lea5"
                     /protein_id="ADJ19197.1"
                     /translation="MRKICVVTATRAEYGLLKCLLEDILNDPELELQIISTGSHLSPE
                     FGYTNRQIVNDGFYINKKIEILLSSDTPIGVSKSMGLAQISFAEAFDELCPDILLVLG
                     DRYELLPIVSAANIAQIPVAHLNGGELTEGAIDESFRHAITKLSQLHFTAMDEYTQRV
                     IQMGESPERVFTVGEVGLDNLSRMTFLSREDFEKAINHKLKKKNILFTYHPETTQTIA
                     SIKNDFQQILQAIDLLKDTLVIFTKANADVGGRLINEMIERYVDKHPDKAIAFASLGQ
                     LRYLSALKYVDAVVGNSSSGIVEAPSFKVATINLGDRQKGRVRARNTIDINVDAEKLL
                     EALQKIETEEFKTMLADVTNPYGDGNSSRKIIDVLKKIDLKALRTKRFYDIQAK"
     gene            6997..7980
                     /gene="weiE"
     CDS             6997..7980
                     /gene="weiE"
                     /codon_start=1
                     /transl_table=11
                     /product="WeiE"
                     /protein_id="ADJ19198.1"
                     /translation="MIYRRNSILGGEQELKEDGLFYGVTNSGRSSLRWLLRSMKLYGK
                     NILVPDFICQVVIDVLLEFEMNIVFYTVHDNFEFSLPKNLNDISALYLVKYFGHASDS
                     FNSAISSSSIPLIIDDVFGVHAPVISPNISWGYFNSLRKITQIADFSYVVTNIPLVDI
                     DKKKLSCFAALKYQGKNMKFNFIYNDSGNESEYLECFNKSERIINDNVGIFYPEDKSI
                     YLVGKFQENYVNDRMIRLKNLAEAERCIARDKKINVSPDYPSFFPVLLNQREKVRGEL
                     MKRGIYLAVHWPNTNQIRNNISDRIISVPLDSRYNSKDILRICDLINQFDD"
     gene            8021..9070
                     /gene="lea6"
     CDS             8021..9070
                     /gene="lea6"
                     /note="putative sugar-phosphate nucleotide transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="Lea6"
                     /protein_id="ADJ19199.1"
                     /translation="MSDEWKKVLLTPAATIRQALEIINRGSLQIALVSSENTLLGSVT
                     DGDIRRGLLRNLSLDDAVEKVMNTSPLTADVSLPKDELLLLLKANKITAIPITENGQL
                     IGLKTLHELLKSKRYDNPVFIMAGGFGTRLKPLTDNCPKPLLKVGNKPILEIIIQRFI
                     DAGFYQFYISTHYLPNMIHEYFGDGSKWNVEINYVHEEEPLGTGGALGLLPKNIKQLP
                     LIMINGDILTNLDINSLLNYHNEQHSLATICVREYNYQIPYGVIEAKGNKIVRMTEKP
                     IYKYHVNAGVYVISPDIFNNVKANTIIDMPSLLQKYIENDEMISIYPLTEYWLDIGQM
                     AEYKQAQKDIVSLGL"
     gene            9072..9773
                     /gene="lea7"
     CDS             9072..9773
                     /gene="lea7"
                     /note="CMP-N-acetylneuraminic acid synthetase"
                     /codon_start=1
                     /transl_table=11
                     /product="Lea7"
                     /protein_id="ADJ19200.1"
                     /translation="MSILAIIPARANSKRLPNKNILKLAGKPLIKWTIDSALQCNDIS
                     KVMVSTDSPKIAELATQFGADVPYLRCAELSGDTASSYDVVIDAISYYRKTGKEFKDI
                     LLLQPTSPLRDVKDINNAISLYRKRDANAVVSVVECEHSPLWCNTLPDSLRMDNFIND
                     EIKKTRSQDLPKYYQINGAIYLIKTESLLREKTFIPKKTFALIMSKKNSVDIDDYYDF
                     LRAEVFCNEDKRSFL"
     gene            9748..10989
                     /gene="wzx"
     CDS             9748..10989
                     /gene="wzx"
                     /note="O-unit flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ADJ19201.1"
                     /translation="MKIKGLFYNLSKPLGYLVGDIVNKAIPFLLLPVIVSKISIETYG
                     YYSVEVVIYSFFCCLLTMGAQAKIILDISRDEIHSNVVTSNVLVLFFLLSVFCGCLLF
                     ILNIFDGLINPNINITLVFFCAIIQSFVNICLTRYQAENRVKIFVAINIIYTLAYFFG
                     VLYSLFIDHWDNVWKNIILFYVLTTITMFFFYFIRKGKVVKLFFFHYISKNKVYETFI
                     FGLYQLPHVLSSWVRLGYDRLVLGELISMSYVGGYSVAVQISLVSSVIFQSLNRFWTP
                     FFIKKLKENSKQKKIIVLGGFGIICITILNILFGYAYFIFFLPTNYSSFSNALPILCM
                     AYMFQGLYFLIVNYIYYHDGNKLISIPSVASIAIHIAVAPVLIKHMGYYGAAISLLIS
                     WIVLFLFTCVIIFYVRRGYRG"
     gene            10982..11914
                     /gene="weiF"
     CDS             10982..11914
                     /gene="weiF"
                     /note="glycosyl-transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WeiF"
                     /protein_id="ADJ19202.1"
                     /translation="MGNIFIVESPFQLSNALLYHKKNDSVIVRLNGENKNDFQIEKML
                     SSFNGKVYIKKASKESKFDLIRFVLFFAVPVLIANLNKKVIIGNYNSLWMRVMGYLFN
                     PFHFAVLDDGLITIRTIKRLDDNISRSGSIKKRFLLLLAPRFITQYKIYSNFIQIYNQ
                     EINKRKRTTRAIKAGRVCFIGSPLFDKNVLTFDFYVKCLAAISDNLKRCGYSIEYYPH
                     RSEKNISYLNVFFDDVIKSDDSIKVYYSASNELPEIFVSFYSSALLNLRSDYPECKFI
                     SYKLDCNEINGKFRYEIMEAYNFLAFSGIEVVTI"
     gene            12009..13196
                     /gene="wzy"
     CDS             12009..13196
                     /gene="wzy"
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ADJ19203.1"
                     /translation="MLTYSVVNWKAKLLYFILFLSFLNAVLRLSEDGLSIYRLFIPLQ
                     IFLIYTLSVTEFKKFIVILLLLLLFGATGCMISTYSLNENNLVFLIHYSILILTFFSS
                     SVLISITGETCFYKFTAFFFGFLIVTGIMDLFGVTFPNIEHIPDAIRGIHRIENDYSL
                     ALVSFVFVVFSISRKKITASILTFFTSAICFYNDSKVALLFVVAGYFCFIFKNIRFRQ
                     KGIQVDIIFFFVIMLIMFLFNYIFSYKIAFPGRDIHIRTNRRACYQIIELNPYGDLYG
                     SISNRTDMVIYTLKDLFSSFGLGIGFGNTLTMLSTAKYAFIGSAKSIHNFPLQFIAGI
                     GLIFSLFLLYSILKQTSLYTKIIFFLMLLASLSQSVGVFSNYYFFCCLFFIILHGKQN
                     GRV"
     gene            13183..13941
                     /gene="weiG"
     CDS             13183..13941
                     /gene="weiG"
                     /note="glycosyl-transferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WeiG"
                     /protein_id="ADJ19204.1"
                     /translation="MDESKISIITPSYNSSEWIEQTYLSIKSQSYSNWEWLVTDDFSC
                     DDTVTLLKKIRAQDPRVKVFQNEKNLGAAVSRNNSLCYAEGEFVAFIDSDDLWYPMKL
                     QTQIEFMKENNINFSFTPYELIDKNGVKLHHSVDTHNIQYVTYYDMLRKKATLGCSTV
                     MLRTSAFDDISMPLIRTGQDYALWLRLLKSGEKAYKLDQILTQYRILPTSISRNKIKK
                     AKRQWAIYREIEALSLTESLECFCFYAYRAVFRK"
ORIGIN      
        1 cgtgagggta aagtcagccg cattgttgaa tttatcgaaa aaccggatca gcctgcagac
       61 gctggactca gacatcatgg ccgtgggccg ttatgtgctt tctgccgata tttggccgga
      121 acttgaacgc acgcagcctg gtgcatgggg acgtattcag ctgactgatg ccattgctga
      181 actggcgaaa aaacagtccg ttgatgccat gctgatgaca ggtgacagct acgactgcgg
      241 taaaaaaatg ggttatatgc aggcgtttgt gaagtatgga ctgcgtaacc taaaagaagg
      301 ggcgaagttc cgtaaaggga ttgagaagct gttaagcgaa taatgaaaat ctgaccggat
      361 gtaacggttg ataagaaaat tataacggca gtgaagattc gtggtgaaag taatttgttg
      421 cgaatattcc tgccgttttt ttatataaac aatcagaata acaacgagtt agcaatagga
      481 ttttagtcaa agttttccag gattttcctt gtttccagag cggattggta agacaattag
      541 cgtctgaatt tttcgggttt agcgcgagtg ggtaacgctc gtcacatcgt aggtatgcat
      601 gcagtgctct ggtagctgtt aagccagggg cggtagcgtg cagggaaaga tcatcagtac
      661 gctttttgta ctaaggaaag attgcgtaac gatgtttttt gtactaaaca atttgcacta
      721 aatgtctcaa taattgagat atctcttatt acctaaaact atttttcata gtttatatat
      781 gatcaaatac tccttatata aataaggaga acaaaatgga acttaaaaaa ttgatggggc
      841 ataaattgtt gggtattcta ctgttgatta tttgtgctgt catcgctgat gcagaaggtt
      901 gggaagatat agagtatttt tgcgaaacgc aactcgattt tttgaagcaa tatggtgatt
      961 tttaagatgg tattccagat catgatacta ttgctagagt tgtattctgt ttttatcgca
     1021 gggatgttca tcgcaatgta ctgaaatcat tgatgcatgt agaataagcg gcacataacg
     1081 catggatgtg gtaattaatg aagacgacaa cagaataaga agatgaaatg aagtaaaatt
     1141 atttttaaaa ataaggaaaa tgaattgaca agatctaata cgctcaagtg tagatgtagg
     1201 acataaaata tgagaagtta ctattgatag aaacgaacaa taatcgttct tttctgatct
     1261 tggtgaaggt gttttatata aaacaatgat tggttacagt atattttctt aaactgaaat
     1321 ctatttcaac ctcatttaaa tgctgcttaa aaatactaat cggtgttgga gaggtgtgat
     1381 tgattaggtg ggagtatatt cttttgttgg tgttttcttt aagaattata tgtcagtaat
     1441 aaataccaac tgactattag aatgttgatt aaatattgat attaatgggt aaatatgaat
     1501 caaatattag aacttattgg acgagaaagt gctctatttg cacaagatat taataaatat
     1561 gagtctgatt tatctcttgc tgtttcagag tctcggtttc ttgtattagg gggggctggc
     1621 tcaacaggac aagcagttgt aaaggaaata tttaaaagaa atccacgtaa gctacatgtt
     1681 gtcgatatta gtgagaataa tctagttgaa ttggtacgtg atatccgtgg ctcatttggc
     1741 tatattacag gtgatttcca gacatttgca ctggatattg gaactgtaga atatgatgca
     1801 tttataaatg atgatggtca atatgattat gttttgaatc tctctgcttt gaaacatgtt
     1861 cgtagtgaaa aagatccgtt tactttgatg cggatgattg atgtcaacat tattaacacg
     1921 gataaaacta ttcagcaatc tatagatgct ggagttaaaa aatatttttg tgtatcaaca
     1981 gataaggcgg caaatcctgt aaatatgatg ggtgcatcta agcgtataat ggaaatgttt
     2041 ttgatgcgaa aaagtgaata tatttctatt tctacagctc gctttgcaaa cgttgcattt
     2101 tctgatggtt ctttgcttca tgggtttaga cagaggctca ctaaaaaaca gccaattgtc
     2161 gctccagatg atataaaacg atatttttta tccccacagg agtctgggga gctttgtctc
     2221 atgtcatgta tttttggtga taatcgagat atatttttcc ccaaattaaa tgagagtttg
     2281 catctgatta cattcgcgga cattgccata aaatatctga agcaatgcaa ctatgaacca
     2341 tatttatgca atagcgaaga ggaggcccga aagttagtta agatattacc aaatcaaggt
     2401 aaatggcctt gcctgctaac aaaaagtgat acaacaggtg agaaagactt tgaggagttt
     2461 tttacagaga aggagaagct agatatgaat tgttttgaaa gtatcggagt tataaagaat
     2521 gatttactat ataacaataa attgttaagt tctttcgagg acgaaattat gcggatgaag
     2581 aacaaaaaag aatggactaa atcacagata gtgaaacttt ttctaacaat gcttccagac
     2641 tttaatcata aagagactgg taagtatctc gatagtaaaa tgtgagagtg gagagatgga
     2701 aacatcattg cttattgaat ttattcggga atgttataaa acctcaaaat ttattccatt
     2761 gcacgcacca atattttctg gtaacgaaag aacttatgta atggaaaccc ttgatagtac
     2821 gtttgtctca agtgttggta aatttgttaa tgaatttgaa cgtaaagtca aagagtttac
     2881 aggaagtgct tatgctgttg ctactgtaaa tggtacagca gcactgcata gtgcactata
     2941 tatggcccaa gttaaacctg gagattttgt tattacacaa gcattaactt ttgttgcaac
     3001 gtgtaattca atttaccatt tgggtgctga gcctatcttt gttgatatat cgcctgtcag
     3061 tttgggatta tgtccgagag ctgttgaacg ttatctggaa gagaatgcag aacttgatga
     3121 aactggctgt attcatcggc agacagggcg aagaattaag actatcgtac caatgcatac
     3181 atttggtcat ccagttgaac tagatgagtt agctgctatt tgctttaaat ggaaattaac
     3241 tttagtcgaa gatgcggcag aaagtttagg ctcattttat aaaggaaggc atacgggaac
     3301 gataggggca ttgggggcaa ttagctttaa tggtaataag ataattacta ctggaggtgg
     3361 tggcatagtt ttgtgtaata gtgaggatct tggtaaacgt ttaaaacatc taacaacaac
     3421 agcgaaaatt cctcatcctt atgagttttt ccatgacgaa gccggattta actatcgaat
     3481 gcccaatatt aatgcggcat taggctgcgc acaaatagaa atgttaaatg agtttttatt
     3541 aaagaaaaga aagttagccc aaatgtataa agatttcttt tcgttaagtg atgttgagtt
     3601 tgtaactgaa cctgaatatg ctcattctaa ttactggctt aattccatta tttgtgtaga
     3661 tcagcagcag cgtaatgaaa ttattgagca aactaataat tcaggtgtga tgacgcggcc
     3721 tgtttggcag ttaatgcatc gtcttcccat gttcaaaaac tctttgtgtg gagaattgac
     3781 ttactctgaa tttattgaag cgcgtttagt aaatttacct agtacaccta taatatgaag
     3841 gtgattgatg gaaataacaa cgttcattga tttatccaaa gatatatatt atcagcaaga
     3901 gtactcagca ttatatgaat gggatggaaa aatatttgaa tatttgtata atgaaaatga
     3961 ctacttcgta aagtttaggg cacttaaacg aaaaattact agtgtggcag gtgaactaat
     4021 taatgaagaa ctttatgatt tagaaacacc atatggctat ggagggcctc gaacgaattg
     4081 tagcgatgat ggttttttag ctagggcttt tgcttcatat cgtgctcatt gtaaacaaca
     4141 taacattgtt agtgagttta ttcgatttca tccatttaat aaattagcag agaacagcat
     4201 gttatttgat atgcatgctt tggagcgtca ggttgtaatt gttgatttga cgcttaatag
     4261 tgaagagcgt cgccaattgt attcaaaaaa aactcgaggc attataaata aagccagtaa
     4321 agagcttact attagtatag atgatgtcga actaagtgag tttacggaaa tgtattatga
     4381 aacaatgaag aaaaataaag ctgatgcttt tttttatttt gatgagtgtt attttgaaaa
     4441 attaatgcag attgatggtg ttaatttaat tgcggctcga aaagataaaa actgtgcgtc
     4501 gattggcttt tttctttgta gtaatgattt ggctcattat catctttctg caaataatca
     4561 aaggttttca aaagaaaatg ggaactattt gcttcttgat gctgcatttg aatatgccaa
     4621 gacaaaagga tgcaaatata tgatgttagg cggaggaaga acatcttctc aggaagatgg
     4681 tttattcaaa tttaaatcta aattttctcc tgcaaccaag ccattttata tcgcaggatt
     4741 gagtttttgg cctgagaaac atcagtatct gaatcagatt tggctcgaga aaaatattaa
     4801 aaacgtaact cctgaaatat tacaattata tagagtttag agggattcat gggaaaaaaa
     4861 gtatttatta ttgcagaggc aggcgtaaat cataatggtg atattgattt ggcaaagaag
     4921 ttagttgatg ctgcagtaca ggcgggggtt gacgctgtca aatttcagac atggaagact
     4981 gagttattag ttactgaaag cgcagaaatg gcagagtatc aaatcgaaaa tacacaagta
     5041 aaagagagcc agtttcagat gcttaagcgt ttggaactga gttataatga ttttgttgaa
     5101 ttaaaaacat actgtgattt taagaatata atatttatgt caactccgga tgaagaacag
     5161 agtgcaacat ttcttgataa tcttcaatct atatttaaaa tcggttctgg tgaattaaca
     5221 aatatccctt ctctacggca tattgcaaga ttttcaaagc cggtaattct atctacgggg
     5281 atgggatatt tatctgaagt tgaacacgct attgaaacat tatgtaatgc aggtttatcg
     5341 ttggatatga ttacagtatt acatgccact acgaattatc ctacagaacc aaaagatgtt
     5401 aatttactgg cgatgagaac tattgcgcaa gcatttcctg gagtcaatgt aggctactcg
     5461 gatcatacca atggtataga aattcctatt gcagctgtag cgctaggagc taaagtcatc
     5521 gaaaaacatt tcacattaga caaaatgatg tgcggtcccg accataaagc tagtcttgaa
     5581 cctcaagagc ttaaacatat ggtaactgca atccgcagta ctgaattggc tctgggtaac
     5641 ggctggaaaa ttccgactga tgttgagatg caaaatagaa atattgtacg aaaaagtata
     5701 gttgcaagta aacctattgt tgccggaacc attattacta gtgatatgtt agaaataaaa
     5761 cgccctggta gtggtatatc gccaagtcga tgggatgaag ttgttggttc tgttgcgaaa
     5821 aaagattatc aaattgggga gttaatttag tgcggaaaat ttgtgttgta acagctacga
     5881 gagccgaata tgggcttttg aaatgcttac tggaggatat tcttaacgat cctgaacttg
     5941 aattacaaat catctcaaca ggtagccatt tatctccaga gtttggttat acaaataggc
     6001 agatagttaa tgatggtttt tacattaaca aaaaaattga gatattactc tcttctgata
     6061 ccccgattgg cgttagtaag agtatggggt tagcgcagat tagctttgct gaggcttttg
     6121 atgagctatg tccagatatt ttacttgttt taggtgatag atatgagctg ttgccaatcg
     6181 tttctgctgc aaatattgcg caaattcctg tggctcactt aaatgggggg gagttaactg
     6241 aaggagccat tgatgagagt tttcgtcatg cgatcactaa gctctcacag ctacatttta
     6301 cagcaatgga tgaatatact caacgagtca tccaaatggg agagtcccca gagcgagtat
     6361 ttacagttgg tgaggttggt ttagataatt tatctcgaat gactttttta tctcgtgagg
     6421 atttcgaaaa ggctattaac cataaattaa agaaaaaaaa tattcttttt acttatcatc
     6481 ccgagacaac tcaaactatc gcatcgataa agaacgattt tcaacaaatt ctacaagcga
     6541 tagatttatt aaaggatacc ttggttatat ttacgaaagc caatgctgat gtcggtgggc
     6601 ggttaataaa tgaaatgatt gaacgttatg ttgacaaaca cccagataag gcgattgcat
     6661 ttgcttcact gggacaatta cgttatttat ctgcactcaa gtatgttgat gctgttgtgg
     6721 gcaactcatc aagtggaatt gttgaggcgc cctcatttaa ggttgccact atcaatttag
     6781 gggatagaca aaaaggacgg gtacgggcga gaaatacaat tgatatcaat gttgatgcag
     6841 aaaagttatt agaggcatta caaaaaatag agacagaaga gtttaagact atgttagcag
     6901 atgtaactaa tccatatggt gatggtaata gttctagaaa aataatagat gttttgaaaa
     6961 aaatagatct aaaggctttg agaacaaaga ggttttatga tatacaggcg aaatagcata
     7021 ttaggaggtg aacaggagtt aaaggaagat gggctttttt atggtgtaac gaatagtggg
     7081 cgttcatcat tacgatggtt actccgatct atgaagttat atggaaaaaa cattttagtt
     7141 cctgatttta tttgccaggt tgttatagat gtattactgg agtttgagat gaatattgtg
     7201 ttctacactg tgcatgataa ttttgaattc tcactcccta aaaacctgaa tgacattagt
     7261 gcactatatt tagtaaaata ttttggtcac gcatctgatt catttaattc agctatctcc
     7321 tcctcaagta ttccattaat cattgatgat gtgtttggag ttcatgcgcc tgtaatcagc
     7381 cccaatatat cgtgggggta ttttaatagt ttgaggaaaa tcacgcaaat tgcagatttt
     7441 agttatgttg ttacaaatat ccctcttgtt gatatagata aaaaaaaatt gtcttgcttt
     7501 gctgctctta agtatcaagg aaagaatatg aagttcaact ttatttacaa tgattctggt
     7561 aatgagagtg agtatttaga gtgttttaat aaaagcgagc gaattattaa tgataatgtt
     7621 ggcatttttt atcctgaaga taaaagtatt tatcttgtcg gaaagtttca agagaattat
     7681 gtaaatgata gaatgattcg gttgaaaaat ctagctgaag cagaaagatg tatcgcacgg
     7741 gataaaaaaa ttaatgtatc ccctgattac cctagttttt ttcctgtctt attgaatcaa
     7801 cgagaaaagg taagggggga gctaatgaaa agaggaatct atcttgctgt acactggcca
     7861 aatacgaatc aaataagaaa taatatatcg gatagaatta tctcagtacc attagattct
     7921 agatataata gtaaagatat tctcaggatc tgtgatttaa ttaatcagtt tgatgattaa
     7981 gtataatata ctaacagttt gatataaaat ggtggattcg atgagtgatg aatggaaaaa
     8041 ggtgttacta acgcctgctg ctactattcg acaagctttg gagataatta atagaggctc
     8101 tttacagatt gctttggttt cttcagagaa cactttattg ggtagtgtta cggatggaga
     8161 tattcgtagg gggctgttaa ggaacctttc actagatgat gctgttgaga aagtaatgaa
     8221 tacatctcct ttaacagccg atgtatcttt gcctaaagat gaattgcttt tattattgaa
     8281 ggctaataaa ataactgcaa tacctattac agaaaatgga caactcatag gattaaaaac
     8341 attacatgaa ttattaaaaa gtaagagata tgataaccct gtatttatta tggctggtgg
     8401 gtttggcaca aggctcaagc cattaacaga caattgtcca aagcctttat tgaaggttgg
     8461 gaataaacca atattagaga taataattca gcgttttatt gatgctggct tttatcagtt
     8521 ttatatatcg acacattatc tacctaatat gattcatgag tattttggtg atggttcaaa
     8581 atggaatgtg gagattaatt atgttcatga agaagagcct ttaggtaccg gaggggcttt
     8641 aggattacta cccaaaaaca tcaaacaatt acctttgata atgattaatg gtgatattct
     8701 gacaaactta gatataaatt cacttcttaa ttatcataat gaacaacatt cgttagctac
     8761 aatatgtgtc cgggaatata actatcagat tccttatggg gttatagaag cgaaaggaaa
     8821 taaaatagta agaatgactg agaagccgat atataaatat cacgtaaatg ctggagttta
     8881 tgttattagt ccggatatat ttaataatgt gaaggctaac actataatag atatgccaag
     8941 cttattacaa aaatatatcg aaaatgatga gatgatttcg atatatcctc ttactgagta
     9001 ttggctggat atcgggcaaa tggctgagta taaacaggct cagaaagata tagtttcttt
     9061 aggtttataa tatgagtatt ttagctatca tacctgcaag agctaatagt aagcgtctac
     9121 ctaataaaaa tattcttaag ttggcaggaa agccattaat aaaatggaca atagattcag
     9181 cgctccagtg taatgatata tcaaaagtta tggtttctac tgatagtcca aaaattgctg
     9241 aattggctac gcagtttgga gctgatgtgc cttacctgcg ttgtgctgag ttgtctggcg
     9301 atacggcctc tagttatgat gttgtcattg atgctatttc gtattataga aagactggta
     9361 aagagttcaa agatattttg ctattgcagc caacatcacc gttgagggat gttaaagata
     9421 taaataatgc aatatctctt tatagaaaaa gagatgcaaa tgctgttgta tctgtagttg
     9481 aatgtgagca ttcaccacta tggtgcaata ccttgcctga cagtttgagg atggataatt
     9541 ttattaatga tgagataaaa aagacaagaa gccaagattt acctaaatac tatcaaataa
     9601 atggagcaat atatttaata aaaacagagt cgctgctgag agaaaaaaca tttataccca
     9661 aaaaaacatt tgcattaata atgagcaaga aaaactctgt tgatattgat gattattatg
     9721 attttttaag ggcggaagta ttctgtaatg aagataaaag gtctttttta taacttgagt
     9781 aagccattag gatatctggt tggcgacatt gtcaataaag caattccatt tcttctttta
     9841 ccagtaattg ttagtaaaat atcaatagaa acatatggat attatagtgt tgaagttgtt
     9901 atatattctt ttttttgttg tttgttgaca atgggggcgc aggctaaaat aatattggat
     9961 atatcaagag atgagatcca ctcaaatgtt gtaacatcta atgtgttggt tttgtttttt
    10021 cttctttctg tcttttgtgg ttgtctttta tttatattga atatatttga cggtcttatt
    10081 aacccaaata taaacataac acttgttttt ttttgtgcga tcatacagtc atttgttaat
    10141 atctgtctga caaggtacca agcagaaaat agggttaaga tatttgtagc cataaatatc
    10201 atatatacgt tagcctattt ttttggggtt ttatattcct tatttataga tcactgggat
    10261 aatgtttgga aaaatattat tttgttttat gtgcttacaa caataacaat gtttttcttc
    10321 tattttatac gaaaagggaa ggttgttaag ctatttttct ttcattatat ctcaaaaaat
    10381 aaagtttacg aaacttttat ttttggcctt taccagctcc ctcatgtttt aagctcttgg
    10441 gtaaggcttg gatatgatag actggtattg ggggaactaa tatcaatgag ctatgttggc
    10501 ggatattcgg ttgcagttca aatatctctt gtgtctagcg tgatatttca atcattaaat
    10561 aggttttgga cccctttctt tattaaaaaa ttaaaggaga atagtaaaca aaaaaaaatt
    10621 attgttcttg gaggttttgg aattatctgc ataacaatac ttaatatact atttggttac
    10681 gcatatttta tttttttctt gcccacaaat tattcatcat tctccaatgc tcttcctata
    10741 ttatgtatgg catatatgtt tcaggggcta tattttttga ttgttaatta tatatattat
    10801 catgatggta ataaattgat atcaattcca tccgttgcct cgatcgctat acatatagct
    10861 gttgccccag ttttgataaa acatatggga tattatggag ctgcaatttc cttattgatt
    10921 agttggatag ttcttttttt atttacatgc gtcattatat tttatgttag aagggggtat
    10981 cgtgggtaat atatttattg ttgagtctcc gtttcagctt tcaaatgctt tactttatca
    11041 taaaaaaaat gatagtgtta ttgtcaggct caacggagaa aataaaaatg attttcaaat
    11101 agaaaaaatg ttatcgtcgt ttaatggcaa ggtatatata aaaaaagcat caaaagaatc
    11161 gaaattcgat ttgattaggt ttgttttatt ttttgccgtc cctgtattga ttgctaattt
    11221 aaataaaaaa gtaattattg ggaattataa ttcattgtgg atgagagtca tgggatattt
    11281 gtttaaccca tttcattttg ccgtccttga tgatgggttg attactatac gaacaataaa
    11341 aaggttggat gataatatat cgcgttctgg atcaattaag aaacggtttt tattactgct
    11401 ggcaccaaga tttataacgc aatataagat atattccaac tttattcaga tatacaacca
    11461 agagatcaat aagagaaaaa gaactacccg agccattaag gcagggcgag tttgttttat
    11521 tggctctcca ttatttgaca aaaatgttct cacatttgat ttctatgtaa agtgcttggc
    11581 tgcgataagt gataacctta agcgttgtgg atatagcatt gagtattacc ctcatcgctc
    11641 agaaaaaaac atctcatatt taaatgtttt ttttgacgat gtaataaaat cagatgatag
    11701 cattaaagtc tattattcag ccagtaatga gttgccagaa atatttgtat cattttattc
    11761 atcggccctt ttaaatttga gaagtgatta tccagagtgt aaatttatat cttataagtt
    11821 agattgtaat gaaatcaatg gtaagtttag gtatgaaata atggaggcat ataacttttt
    11881 ggcattctct ggtattgagg ttgtaactat ataggaaaat aagatagaaa agggatattg
    11941 ttggagtcaa tatagaacag aaattatagt ctaaactaat taatcactat gaaggtctat
    12001 gtaattaaat gttaacatat tctgtcgtta actggaaagc aaaattatta tatttcattc
    12061 tttttctgag tttccttaat gctgttttga ggttatcaga ggatggattg tcgatttatc
    12121 gattatttat ccctttgcaa atatttttaa tttatacatt aagtgtcacc gagttcaaaa
    12181 agtttatcgt aattctacta cttttattat tgtttggtgc tactggatgt atgatatcca
    12241 cttattctct taatgagaat aatcttgtat ttctaataca ttactctata cttatactca
    12301 cttttttctc ttcttctgtg ttgatatcta ttactggtga aacttgtttt tacaaattca
    12361 ccgcgttctt ttttggtttt ttaattgtta ctggtattat ggatttgttt ggtgttacat
    12421 ttcctaatat agaacatata ccagacgcta tacgaggaat acatagaata gagaatgatt
    12481 attcacttgc gctggtttcg tttgtttttg ttgtgttttc catctctagg aaaaaaataa
    12541 ctgcttcaat ccttactttt tttacgtctg cgatttgttt ttataatgac tctaaagttg
    12601 cattactttt tgtcgttgct ggttattttt gttttatatt taaaaatata aggtttagac
    12661 aaaagggaat tcaggtggat attattttct tttttgtcat aatgctgatt atgtttttgt
    12721 ttaattatat tttctcgtat aaaatagctt tccctggtag agatatacat attagaacta
    12781 ataggagagc ctgttatcag attatagagt taaatccata tggtgatctg tatggttcaa
    12841 tatctaatag aactgatatg gttatatata cattgaaaga tttgttttct tcatttggct
    12901 taggcattgg gttcggcaat actttgacaa tgttatcaac tgccaagtat gcatttattg
    12961 gtagcgccaa aagtatacat aattttccgt tgcaatttat tgcaggaatt ggtctgattt
    13021 tttctttgtt tttactgtat tcaattttaa aacaaacatc attatataca aagataattt
    13081 tctttttgat gcttttggcg agtctttctc aatcagttgg cgttttttca aattattatt
    13141 tcttttgttg tctgtttttt ataatattac atggtaaaca aaatggacga gtctaaaatt
    13201 tcaattatta caccatcgta taactccagt gaatggattg aacaaactta tcttagtata
    13261 aaatcacaga gttattctaa ttgggagtgg ttagtaacag atgatttttc ttgtgatgat
    13321 actgtcacgt tgttaaaaaa aatacgtgca caagatcccc gtgttaaagt ttttcaaaac
    13381 gaaaagaact taggagctgc agtttcgaga aataattcat tgtgttatgc agaaggtgaa
    13441 tttgtcgcct tcattgatag tgatgattta tggtatccaa tgaaactgca aactcagatt
    13501 gagtttatga aagaaaataa tattaatttc tcatttacac cttatgaact tattgataag
    13561 aatggagtta aattgcacca ttctgtagat acccataata ttcaatacgt tacctattat
    13621 gatatgttgc gaaaaaaagc cactttaggg tgttctactg taatgttacg taccagcgca
    13681 tttgacgata tatctatgcc tttgattcgt acaggtcaag attatgcatt atggctaaga
    13741 ctattaaagt caggggaaaa agcatacaaa ctagaccaga ttttaactca atatcgcatt
    13801 ttaccaacct caatatctag aaataaaata aagaaggcta aaaggcaatg ggctatatat
    13861 agagaaatag aagctcttag tctgaccgag tctttagagt gtttttgttt ttatgcatat
    13921 agagctgtgt ttcgtaaata agtcactgta tgggatgtca tcttaaaata tgttatttta
    13981 gggtaaggtt ttatatgttc ttatttgtgt tgttacttca tttataatgt gatttattgc
    14041 gagctttgtt ttatgtattt ctgttgctat ttcttatatg ggggctgggg atatgttgct
    14101 tttataaaac tcatgtttga cttaaaaaga gtgtgtgatt tatttgtggc ggcgtttaag
    14161 tttaataatg gtagtcaatc ctgttgactc tgtatcagta atattgataa tgatatatag
    14221 cttggaagta tataggtaat aaatataata cataacaaga aatattttat gggtaagtct
    14281 attatgatta ggtgttattc ttgataatat atccaaaata atgataaaac atgaaaatat
    14341 tagaggttag ctcacataag tttttgctgg gtactgactt ccatgttttc tctttttatt
    14401 agtagtaaat acaactttgt cttggtatta atagcattaa tgaaattcag cactacacac
    14461 attcgtgcaa cttgagataa catctcaatc acattcaagc cgcgcaaccc cgcggtgacc
    14521 acccctgaca ggagtaaaca atgtcaaagc aacagatcgg cgtcgtcggt atggcagtga
    14581 tggggcgcaa ccttgcgctc aacatcgaaa gccgtggtta taccgtctct attttcaacc
    14641 gttcccgtga gaagacggaa gaagtgattg ctgaaaatcc aggcaagaaa ctagttcctt
    14701 actatacggt gaaagaattc gttgaatcac ttgaaacgcc tcgtcgcatc ctgttaatgg
    14761 tgaaagcagg tgcaggcacg gatgctgcta ttgattccct taaaccatac cttgaaaaag
    14821 gcgacatcat cattgatggt ggtaacacct tcttccagga caccattcgt cgtaatcgtg
    14881 agctttctgc agaaggcttt aacttcattg gtactggtgt ttccggtggt gaagaaggtg
    14941 cgctgaaagg tccttctatc atgcctggcg gtcagaaaga agcctatgaa ctggttgcgc
    15001 cgatcctgac caaaattgct gccgtagctg aagatggcga atcgtgcgtt acctatattg
    15061 gtgcagacgg tgctggtcac tatgtgaaga tggttcacaa cggtattgaa tacggtgata
    15121 tgcagttgat tgctgaagct tattcactgc tgaaaggtgg cctgaatctc tctaacgaag
    15181 aactggcaca gacctttacc gagtggaata acggtgaact gagcagctac ctgatcgaca
    15241 tcaccaaaga tatcttcact aaaaaagatg aagatggtaa ctacctggtt gatgttattc
    15301 tggatgaagc tgccaacaaa ggcacgggta aatggaccag ccagagtgcg ctggatcttg
    15361 gagagtcgct gtcgttgatt accgagtctg tgtttgctcg ctatatctct tctctgaaag
    15421 agcagcgtgt tgccgcgtct aaagtactgt ctgggcctca gcctcggcca gcaggtgaca
    15481 aagcagagtt catcgagaaa gttcgccgtg cgctgtatct tggcaaaatc gtttcttatg
    15541 ctcagggctt ctctcaactg cgtgctgcgt cagaagagta ccactgggat ctgaactacg
    15601 gtgaaatcgc gaagattttc cgtgctggct gcatcatccg tgcgcagttc ctgcagaaaa
    15661 tcaccaatgc ctatgcagaa aatccaaaaa tcgctaacct attgttggct ccgtacttta
    15721 agaaaatcgc tgacgactac caacaggcac tgcgtgatgt cgtcgcttat gctgtgcaaa
    15781 acggtatccc ggttcctacc ttcgctgctg cggttgccta ttacgacagc taccgcgccg
    15841 ctgttctgcc tgcgaaccta attcaggcac agcgacta
//

</pre>