<pre>LOCUS       KJ755559               13035 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain E 1585-68 serotype O156:K-:H47 O-antigen
            gene cluster, complete sequence.
ACCESSION   KJ755559
VERSION     KJ755559.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13035)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13035)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Baranzoni,G.M.,
            Liu,Y. and Debroy,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E 1585-68"
                     /serotype="O156:K-:H47"
                     /db_xref="taxon:562"
     misc_feature    1..13035
                     /note="O-antigen gene cluster"
     gene            283..1422
                     /gene="wzx"
     CDS             283..1422
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AIG62552.1"
                     /translation="MQQLAELGLGQTLRLHIAHAFKMDESGWCFNSKKEIKQYYSLSV
                     KWFLVVALCTIIIIGPAGIYYYHSYQGDIIWEGPWVSLVLVTALTIIITPIQITIESL
                     QHQISVFKAQTYASIINSISLCLLLFSGFGLYAIALSLFISNLTVLIFLWGKIKWLRN
                     NLMPVLVDTNFFVVFKEIWPLFSRVAVVSVFGFLFWNGFNLIAFKVYEPSYAGQVILS
                     ITLARAGYGFSESILYGQITYISNLISNKKYNEAMIVYKKNTYIALTILLIGYVFFLL
                     LMYLFPEFYLFKKIVGSSYLISIFVFFIILLFLTSGNNYVRCFKTEPFVKVSIFHGVM
                     VPTLFYLSSVFYEGYYLYPCSLVLIVSLILSKRISNKYFRMSGIN"
     gene            1436..2398
                     /gene="wzm"
     CDS             1436..2398
                     /gene="wzm"
                     /codon_start=1
                     /transl_table=11
                     /product="capsular exopolysaccharide biosynthesis protein"
                     /protein_id="AIG62553.1"
                     /translation="MNKKDNFVNIFDTSIASLNVGDYIIMDAVTKQLSTISNLPQTVT
                     LPTHDHFGREGRRLLSLAKYSIVGGTNLLSSHVTQYRQWRFRMTDLLFLKKCILMGVG
                     WWQYQDKPDRVTKFILKHILHNEMLHSVRDSFTLKQLHSIGITNVINTGCPTMWDLTP
                     EHCCNIPREKGRRVLFTLTDYNQDLSADALLINTLKRHYDEVLFWPQGSEDIHYMNTF
                     STDIRNGLKILRPSLSELNANLMLKETDYVGTRLHAGIRALQLRARTIIIGVDNRAIE
                     KAKDFNLPVLKRDAILELENMIVEPLITQINMPWGNIEKWKDQF"
     gene            2449..3690
                     /gene="mshA"
     CDS             2449..3690
                     /gene="mshA"
                     /codon_start=1
                     /transl_table=11
                     /product="D-inositol 3-phosphate glycosyltransferase"
                     /protein_id="AIG62554.1"
                     /translation="MKILILNHSDNVGGAARAAYRLHMLFKQKFANSEMFVRVKKSDS
                     SSVFTPKGIYSFLSKIRYPLSKFILKKLLHIWKQDLISLNMIPSSLSRIINESDADIV
                     NIHWIAGETISFKDLSRIKKPLVWTMHDMWPFIGAEHYIDESRTDWLCGYKNLFGKFG
                     IINRYLWRKKLKTYSRLNICFVSPSKWLAEKAKLSGLLQGMRIEVIPNPINFDIFKPL
                     DKEFSRKALNLSLSSKIILFGAVGGVNDQRKGYTLLYESLMHLSDKISNKENIECVFF
                     GQSEQPKEFAPLPFKCTWFGVVNDDVSLSLLYNAADITVVPSRMENLPQICTEAQSCG
                     CPVVAFNTTGISETIEHNKTGYLANAFDTLDLSKAMYTLLFNNDLNKMFSANASERAR
                     FLWNGDSIAKKYESIFSSLKE"
     gene            3700..4905
                     /gene="wzy"
     CDS             3700..4905
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AIG62555.1"
                     /translation="MENQHVYFYKKSNKANVSIVALIFTYISIIGWSPFFSGYLAVPL
                     FLTYHIPFLLLLAYFMLTQKISSALISLSIIMIILISALTLLTESLINFNRYIFEPLT
                     FLLCYYCTVSRKLAEKICTYASCVVIIGIVLSVISFCYAYAGGNEILTFHNVDGRLNS
                     LYLTSFSNFKIGNIIRPSFIYDEPGAFSFVICFVVILREVLGRKYNWVYLLGGMITLS
                     LTQFIILSLYIIVKNWKNIKQILVLCFALFGLIMFTYNNNEFDFFYNRFNTENGTFSG
                     DNRSMQISNYLNVVNENMFLLGDYKCHDNINQRCESHGDISSSIVTPIYKGGILQLLL
                     QIITHAIIFWLALRERKLIFPALVLTILLLQRPFFDVVGYSVMIYYSIFLMFNFKVFK
                     DDYYHSRKK"
     CDS             4877..5584
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="AIG62556.1"
                     /translation="MIIITVVKNDPDGLLKTYNSVISEIGSGLKCSRWIIKLSSDADV
                     SLKKIINLPDYCHVYTDYDKGIYSAFNQAISKIPSSHENEYVMFLNAGDEIINGQEIN
                     KLLDLQRDNIDIFIACAKIQGKEKIIYPISSLIGVGGLNFCHQSILYRVKILKEIPFP
                     EKYKIAGDYAHYLKIQDRDKIICTLIISTFSLDGLSGRSVYKTRLENFKAGFEHNGIR
                     EIFNFVKMIIIRVLDKI"
     gene            5581..6660
                     /gene="gtf1"
     CDS             5581..6660
                     /gene="gtf1"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="AIG62557.1"
                     /translation="MIAFFIGPSTGKVTGQSIAFNIIYDNYSGKKYLINYPDPDEGFL
                     SLFKGYIQSFFVFIFINLLFFFKKKTVYITSSRTLIGFFRDAIYIIVGKLFFAKVVNH
                     LHGADFKYFRNEQCYIIKKIIDYVYNKIDVSIVLTARMKEQYNIYRKMKIKVIGNCYF
                     GLSYRQEKKIFNNETLKLVFLSNLLYSKGIVHLINVVKRINNEGIKVSLEIAGKILSD
                     EYVDSCKLKEILEAKIENCPYIKYHGPLNSEKKSALLNRSDVFVLPTFYRSEAQPISV
                     LEAMYFGCVIVTTAHNYMPDFVTAKNGIIINPDDEDDLYEALLKINNMKSIFSDVAAY
                     NACYVKKNFSPENYINKITSVIIED"
     gene            6661..7779
                     /gene="gmd"
     CDS             6661..7779
                     /gene="gmd"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="AIG62558.1"
                     /translation="MKKAFITGITGQDGSYLAEFLLDKGYEVHGIKRRASSFNTERVD
                     HIYQDRHNQNPNFFLHYGDLTDTSNLIRLIKEIQPDEVYNLGAQSHVAVSFESPEYTA
                     DVDAMGTLRLLEAIRICGLEKKTRFYQASTSELFGLVQEIPQRETTPFYPRSPYAVAK
                     MYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANISQGIEKCLYLG
                     NMDSLRDWGHAKDYVRMQWMMLQQDHPEDFVIATGKQISVREFVRMSAKEVGLELEFS
                     GQGVDEIATVVNKTSDCAIGVSIGDVIVRVDPRYFRPAEVETLLGDPAKAKKVLGWEP
                     EITVEEMCAEMVASDLAKAKQHALLKSHGYDVAVSLER"
     gene            7783..8748
                     /gene="fcl"
     CDS             7783..8748
                     /gene="fcl"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="AIG62559.1"
                     /translation="MKKKRIYVAGHRGMVGSAICRQLSLRDDIELVVKTHKELDLTVQ
                     KDVDAFFAQEKIDQVYLAAAKVGGIYANNTFPAEFIYQNLMIESNIIHSAHKAGIQKL
                     LFLGSSCIYPKFAEQPMNESALLTGILEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGINDNFHPENSHVIPALMRRFHEAKESGAPEVIVWGTGTPMREFLYVDDMA
                     AASVHVMELDEAIYQQNSQPMLSHINVGTGVDCSIREMAETMASVVGYQGKIVFDVTK
                     PDGTPRKLMDVTRLKNLGWQYQYNLHEGLSLTYKWFIENINSFRG"
     gene            8751..9212
                     /gene="gmm"
     CDS             8751..9212
                     /gene="gmm"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="AIG62560.1"
                     /translation="MNKRLERELFKTIVEHTPLISIDLIIRNDKGEALLGQRLNRPAQ
                     NYWFVPGGRIYKDESFEIAFKRITFEEVGVQISLDDALFLGVYEHFYNDNFSEAEFST
                     HYVVHGYEIQLKPQQLHLPTVQHNSYKWFDVVTLLNSTTVHQYTKNYFIPR"
     gene            9218..10624
                     /gene="manC"
     CDS             9218..10624
                     /gene="manC"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase 1"
                     /protein_id="AIG62561.1"
                     /translation="MLLPVVMAGGSGTRLWPLSRTLYPKQFLSLNSRLTMLQETLRRL
                     DKVEHKPALVICNESHRFIVAEQLRKEGLKHSGILLEPVGRNTAPAVALAALQAMVTG
                     DDPILLVLAADHEIQNEDNFIDAVLAAKNFAEQGKLVTFGIVPTSPETGYGYIKSGEY
                     LDGKGYKVAAFVEKPELHVAQQYISDGGYLWNSGMFMFRASVFINELKKFRPDILASC
                     QRSLSSSIQDLDFIRLDNASFSCCPEESIDYAVMEKTAEAVVVPLNAQWSDVGSWSAL
                     WEISSKDQSGNAIRGDVLVEDATDSYLYSQHRLIGAVGIKDLVVVETKDAVLVAHKDK
                     VQQVKNIVAQLKKNNRTEYLQHREIFRPWGSHDTIAEGPRFQVKHVIVLPGHITAKQI
                     HYHRTEHWIVVSGTAKVHLEDKTYLVSENESTYIPVGVPHAIENPGKIPLEIIEVRSG
                     VYLEEDDVIRVSSSGVGY"
     CDS             10624..11370
                     /codon_start=1
                     /transl_table=11
                     /product="PGL/p-HBAD biosynthesis
                     glycosyltransferase/MT3031"
                     /protein_id="AIG62562.1"
                     /translation="MRISIITATYNSEKTLLDTLLSLEKQTYPDIEYIVVDGASNDNT
                     IKLIKSNCTKVSKIICEPDKGIYDALNKGIQAASGDVIGFLHSDDLLAYDDAIADIAK
                     TFEITGCDAIYGDLEYVAQNDTTKRIRLWKSGSFSRFKMKVGWMPPHPSFYMKRKCYR
                     QFGCFSLDYRISADYDSLLRYILKQRISTEYLPKVLVKMRVGGISNRSVSSMVKKSME
                     DIRVMNQNGIIWPIALVYKNISKLPQFIKK"
     gene            11376..12800
                     /gene="glmM"
     CDS             11376..12800
                     /gene="glmM"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="AIG62563.1"
                     /translation="MLNVKKIINDSNIAFGTSGARGLVIDFSHDVCAAFTHAFLSVID
                     DKYNFNKVALAIDNRPSSYEIAQACAFAIKQHGFTVEYHGVIPTPALAHYSMQKNIPC
                     IMVTGSHIPFDRNGLKFYRPDGEITKEDELAIINSEYTFSPVGVLPHLETSTEGADYY
                     LERYVSLFNPDILKGKRIGLYEHSSAGRDLYAPVFNQLGAEVISLGRSDEFVPIDTEA
                     VSDEDRILAREWSKKYNLDAIFSTDGDGDRPLVADENGEWLRGDILGLLTAIELNIKA
                     LAIPVSCNTAIEQSNKFASIQRTKIGSPYVIAAFTDLAKQFNSVAGFEANGGFLLASD
                     LQINGKELKSLPTRDAVLPALMLLIASRNSTISQLINNLPQRFTWSDRVKNFPSDSSQ
                     QIIKNAISSPNNFFNSLGYESLSCSAIDETDGARFTLNNGDIIHLRPSGNAPELRCYA
                     EASDENQAKQYVTNVLGNITSLIS"
ORIGIN      
        1 tcatggtagc ttaaagccag gggcggtagc gtgggtaatg tttactacct atatagttac
       61 gtgcaatgaa aaataagaat aagtgctgaa tgattttgat tatgaatatt ttagattttt
      121 tataaggagg atattataaa tataggaact aaaatctcta tttatgcggc attatctaga
      181 ggtcttaaaa tattaagtgg acccataata attcttgttg tttcaaagac attaagttca
      241 gaggaaattg gattttatta tacttttttc agcttagtaa ctatgcagca gttagctgaa
      301 ttaggtttag ggcaaacatt aagattgcat attgctcatg cgtttaaaat ggacgagtcg
      361 ggatggtgtt ttaactcaaa aaaagaaatt aagcaatact attctctaag tgtaaaatgg
      421 ttcttggtcg ttgcgttatg tactatcatt attattggtc ctgcgggtat ttattattat
      481 cattcttacc aaggggatat tatttgggaa ggcccatggg tatcattagt attggttaca
      541 gcactaacaa ttataataac gcctatacaa atcacaattg aatctctaca acatcaaatt
      601 tcagtattta aagcacagac atatgcatca attatcaata gtatatcatt atgtctgtta
      661 ttgttttctg gatttggtct atatgcaatt gctttaagcc tatttatcag taacttaaca
      721 gtcttaatat ttttatgggg gaaaataaaa tggctgagaa ataacctgat gcctgttctt
      781 gttgatacaa atttttttgt tgtttttaaa gagatctggc cgctgttttc tcgtgtagct
      841 gttgtatctg tatttggttt ccttttttgg aatggattta atctaattgc atttaaagta
      901 tatgagccct catatgcagg acaagtaatt ttatccataa ccttagcgag agctggctat
      961 ggattttctg agtcaatttt atatgggcaa ataacataca tatctaattt gatttcgaac
     1021 aaaaaatata atgaggctat gatagtttat aaaaaaaata catatatagc actaacgatt
     1081 ttgttaatag gatatgtgtt ttttttactt cttatgtatc ttttcccaga attttactta
     1141 tttaaaaaaa tagtaggctc aagctattta attagtatat ttgtattttt tataatactt
     1201 ctttttttaa cctcaggaaa taactatgtc agatgcttta aaacagaacc atttgtaaaa
     1261 gtgtccatct ttcacggggt catggtacca acattatttt atttatcatc agttttttat
     1321 gagggttatt atttatatcc atgttcattg gtgttgattg tttctctaat tttatcaaaa
     1381 agaatatcaa acaagtattt tagaatgtcc ggcattaatt aataataggt gaaaagtgaa
     1441 taagaaagat aactttgtga atatttttga cacaagtatt gcatcattga atgttggtga
     1501 ttacattatt atggatgctg tgaccaaaca gttatctaca ataagtaatt taccgcagac
     1561 tgttacatta ccaacgcatg accattttgg tagagaaggg cgacgtttac ttagcttggc
     1621 taaatattct attgttggcg gcactaattt attatcatcg catgttacac aatatcgcca
     1681 atggcggttt cgtatgacag atcttttatt tttaaaaaaa tgcatattaa tgggagttgg
     1741 ttggtggcaa tatcaagata aaccagacag agttacaaaa tttattttga agcatatatt
     1801 acacaatgaa atgttacact cagttcgtga ttcatttaca ttgaaacaac ttcattccat
     1861 tggcattact aatgtcataa ataccggttg tccgacaatg tgggatttga caccagagca
     1921 ctgttgcaat attccaagag aaaagggaag acgtgtatta tttacattaa cagattataa
     1981 tcaagactta tcagcggatg cattattaat caatactctt aaaagacatt atgatgaggt
     2041 attgttttgg ccacagggga gtgaagatat tcactatatg aatacattca gtacagatat
     2101 tagaaatggg cttaaaattt tacgaccatc attatctgaa cttaatgcaa atttaatgct
     2161 taaggaaact gattatgttg ggacacgatt acatgcgggg attcgtgcat tgcaacttcg
     2221 cgcaagaact ataattatag gtgttgacaa tagagcaata gagaaagcga aagactttaa
     2281 tcttcctgta ttaaaacgag atgctatttt ggagctggaa aatatgatag ttgaaccact
     2341 tattactcaa attaatatgc cgtggggaaa tattgaaaag tggaaagatc aattttaata
     2401 ttattctttt ttgttcagta gagtttttta agataattta gggaccggat gaaaatatta
     2461 atattgaatc attcagacaa tgttggcggg gctgctaggg cagcctaccg attgcatatg
     2521 ctatttaagc aaaaatttgc taatagtgag atgtttgtta gagtaaaaaa aagtgatagc
     2581 tcaagtgtat ttaccccaaa gggcatttat tcttttttat caaagatacg atacccactt
     2641 agcaagttta ttttgaaaaa attattacat atctggaagc aggatttaat tagtttaaat
     2701 atgatcccat cttctttgtc aagaataatt aatgaatccg atgcggatat tgtaaatatt
     2761 cattggattg ctggagaaac aatatcattt aaagacctat cacgaataaa aaaaccttta
     2821 gtctggacca tgcatgatat gtggccattc attggcgctg aacattacat agacgagtca
     2881 agaactgatt ggttgtgtgg gtataaaaac ctattcggaa agtttggtat aataaatagg
     2941 tacttgtgga gaaaaaaact aaaaacttac agtcgactaa atatatgttt cgtatctcca
     3001 agcaaatggc ttgcagaaaa agctaagctg agcggtctat tacaaggtat gcgaattgaa
     3061 gtaattccta atcctataaa tttcgacata tttaagcctc tagataaaga gttttcgaga
     3121 aaagcattaa atctatctct atcatcaaag ataattttat ttggtgctgt aggaggtgtt
     3181 aatgatcagc gtaaaggtta cactctacta tatgaatcct tgatgcacct ttcggataaa
     3241 ataagtaata aagaaaatat tgagtgtgtt ttttttggac agagtgaaca gccaaaagag
     3301 tttgctccac ttccatttaa atgtacctgg ttcggtgtgg tcaacgatga tgtttcatta
     3361 tcgttgttat acaacgctgc tgatattact gttgtgccat cacgaatgga gaacttgcca
     3421 cagatatgta cagaagcaca atcgtgtgga tgccctgtcg ttgcattcaa cactactgga
     3481 ataagtgaaa caattgaaca caataaaaca ggttatttgg caaatgcatt tgatacatta
     3541 gacttatcta aagcaatgta tacactcctt tttaataatg atttgaacaa aatgttttct
     3601 gcaaatgcaa gcgaaagagc gcgttttctt tggaatggtg atagtattgc caaaaaatat
     3661 gaatccattt tctcaagttt aaaggagtga tctgagtaag tggaaaatca acacgtttat
     3721 ttttataaga aatcaaataa agcaaatgtt tcaattgttg ccttgatttt tacatatatc
     3781 tcaattattg gttggagccc tttttttagt ggatatttag ctgtaccatt atttctaaca
     3841 tatcatatac cctttttatt attgcttgct tactttatgt taacacaaaa aataagtagt
     3901 gctttaattt cattatcgat aataatgatt atacttattt ctgcactaac attgttaaca
     3961 gaatcattaa ttaattttaa tcgttatatt tttgaaccgc tgaccttttt gctttgctat
     4021 tattgtacag taagtagaaa acttgccgaa aaaatatgca catatgcatc atgtgttgta
     4081 atcataggca ttgtgctatc agtaatttct ttttgttatg cgtatgcagg aggtaacgaa
     4141 atattaactt ttcataatgt cgatggacga ttgaatagtt tatatctaac ttcatttagt
     4201 aattttaaaa taggaaatat tatccgacca tcatttatat atgatgaacc cggggcattc
     4261 agttttgtta tatgttttgt cgttatttta agggaggttt tgggaagaaa atataattgg
     4321 gtttaccttc ttggaggcat gattacatta agtttaactc aatttatcat attatcacta
     4381 tatattatag ttaaaaattg gaaaaatatt aaacaaatcc ttgtgttgtg ttttgcatta
     4441 ttcgggttga taatgtttac atataataat aatgaattcg attttttcta taatagattc
     4501 aatacggaaa atggaacatt tagcggtgat aatagaagca tgcagatctc aaattattta
     4561 aatgttgtca atgagaatat gtttcttctg ggggactata agtgtcatga taacataaat
     4621 cagagatgtg aaagtcatgg tgatatttca tcaagtatag taactccaat atacaaaggc
     4681 ggtattctac aactattact acaaattatt actcatgcaa taatattttg gttggcactc
     4741 cgagaaagaa aactcatatt cccagcctta gtattaacaa tactactgct tcaaagaccc
     4801 ttttttgatg tagtgggata ttcagttatg atctattatt ctattttttt aatgtttaat
     4861 tttaaagtgt ttaaagatga ttattatcac agtcgtaaaa aatgatccag atggactttt
     4921 aaagacctat aattctgtaa tatctgaaat tgggagtgga ttaaagtgca gtcggtggat
     4981 tattaaacta agcagcgatg cagatgtctc tttaaaaaag attatcaatc tgcctgatta
     5041 ttgccatgta tatactgatt atgataaagg tatttactca gcttttaacc aagcaatttc
     5101 aaaaattcca tcatcgcatg aaaatgaata tgtaatgttt ttaaatgctg gtgatgaaat
     5161 tataaatgga caagagataa ataagcttct tgatctgcaa agagataaca tcgatatttt
     5221 tattgcttgt gctaaaatac aaggtaaaga aaaaataata tatccaatct catctttaat
     5281 cggcgttgga ggattaaatt tttgtcacca gtctatttta taccgagtaa aaatattgaa
     5341 agagattcct tttccagaaa aatataaaat agcaggagat tatgcgcatt atttaaaaat
     5401 acaagaccga gataagatta tatgtacttt gattatttct actttttctc tagatggttt
     5461 atcagggcgt agtgtttata aaactcggct agaaaatttc aaagctggtt tcgagcataa
     5521 tggtataaga gaaatattta attttgtcaa aatgattatt ataagagtat tggataaaat
     5581 atgattgctt tttttattgg accatcaact ggaaaagtta caggtcaatc aatagccttc
     5641 aatataatat atgataacta ttcaggtaaa aaatatctta taaattatcc agatccagat
     5701 gaagggtttt tatctttatt taaaggatat attcaatcgt tttttgtttt cattttcata
     5761 aatttgttat ttttttttaa aaaaaagact gtttatataa caagcagtcg aactctgatt
     5821 ggtttttttc gtgatgctat ttatattata gtcgggaagt tattttttgc aaaagttgtt
     5881 aatcatttgc atggtgctga ttttaaatat tttagaaatg agcaatgtta tataattaaa
     5941 aaaataattg attatgttta taataagata gatgtttcaa ttgttctcac tgcaaggatg
     6001 aaggaacaat ataacatata tcggaagatg aaaatcaaag taataggtaa ctgttatttt
     6061 ggtcttagtt acaggcaaga gaagaaaatt tttaataatg aaacgctaaa actagttttt
     6121 ctttctaatt tattatactc caaagggatc gtacatctaa taaatgtggt aaaaagaata
     6181 aataatgaag gtataaaggt aagtctcgaa attgcgggaa aaatattgag tgatgaatat
     6241 gttgattcgt gtaaattgaa agaaatatta gaggctaaaa ttgaaaattg tccatatatt
     6301 aaatatcatg gacccctgaa ttctgaaaag aaaagtgctt tactaaacag atctgatgtg
     6361 tttgtgttgc caacattcta tcgctctgaa gctcagccta tttctgttct tgaagcaatg
     6421 tattttggtt gtgtgattgt aacgacggct cataattata tgccggattt tgttacggcg
     6481 aagaatggta tcattattaa tcctgatgat gaggacgatt tatacgaagc tttattaaaa
     6541 atcaataata tgaagagtat attttctgat gttgcagcat ataatgcctg ctatgttaaa
     6601 aaaaattttt ctccagaaaa ttatataaac aaaattactt cagtaataat agaggattaa
     6661 atgaaaaaag catttattac aggcatcacc gggcaagatg gctcttattt ggctgaattt
     6721 cttcttgaca aaggctatga agtgcatggc attaagcgtc gagcatcttc gtttaacaca
     6781 gaacgtgttg atcatattta tcaagatcgc cacaaccaaa atccaaattt ctttcttcat
     6841 tatggtgact taacagatac atcaaacctg atacgtctaa tcaaagaaat ccaaccagac
     6901 gaagtttata accttggcgc tcaatctcat gtagcggttt cattcgaatc acctgaatat
     6961 actgctgacg tagatgcgat ggggacttta cgtctgctgg aagcgattcg tatttgtggg
     7021 ctagagaaaa aaacacgttt ttatcaggca tcaacttctg aactcttcgg cttagttcag
     7081 gaaatcccgc agcgagaaac aacaccattc taccctcgtt cgccttatgc tgttgcgaag
     7141 atgtatgcat actggattac tgtaaactat cgtgaatcct atggaatgta tgcctgtaac
     7201 ggtattctct tcaaccatga atccccgcgt cgtggtgaaa cattcgtaac acgtaaaatc
     7261 actcgtgcta ttgcaaatat ttcgcaggga atcgaaaaat gtctctatct tggcaatatg
     7321 gattcactgc gcgactgggg acatgcgaaa gattacgtgc gtatgcagtg gatgatgttg
     7381 cagcaagatc acccagagga ttttgttatt gcaacaggca aacaaatttc cgtacgtgaa
     7441 tttgttcgta tgtcagctaa agaagtcggt ctggagttag aattctccgg acaaggtgtt
     7501 gatgaaatag caactgttgt gaataaaaca tctgactgtg ctattggtgt tagtattggg
     7561 gatgtgatcg ttcgtgttga tcctcgttat ttccgtcctg cagaggttga aactcttctt
     7621 ggtgatccag caaaagcgaa aaaagtattg ggttgggaac cagagattac agttgaagag
     7681 atgtgtgcag aaatggttgc cagtgatctg gcgaaagcaa aacagcatgc actactgaaa
     7741 agccatggtt acgatgttgc agtttctctg gagcggtaag gtatgaaaaa aaaacgtatc
     7801 tacgttgctg gtcaccgagg catggttggc tctgctattt gccgtcaatt atcactgcgt
     7861 gatgatatcg aattagtggt caaaacacac aaagaactcg atctaaccgt acagaaggat
     7921 gttgatgcat tttttgcgca agagaaaatt gatcaggttt atcttgctgc ggctaaagtt
     7981 ggtggcattt atgccaacaa tacatttccg gctgaattca tctatcagaa tctcatgatt
     8041 gagagtaata ttattcattc agctcacaag gccggaattc aaaaattact ttttttaggc
     8101 tcaagctgta tttatcctaa attcgcagag cagccgatga acgagtctgc gcttttaacg
     8161 ggcatacttg agccaactaa tgagccatac gcaattgcta aaattgcggg cataaaatta
     8221 tgtgaatctt ataaccgtca atatggccgt gattatcgca gtgtaatgcc tactaatctt
     8281 tatggcataa atgataattt ccaccccgaa aactctcatg ttattccggc actcatgcgt
     8341 agattccatg aggcaaaaga gagtggtgca ccagaagtta ttgtctgggg aacaggaaca
     8401 ccgatgcgtg agtttttata tgttgatgat atggctgccg catctgttca tgtaatggaa
     8461 cttgacgaag caatttatca acaaaattca cagcctatgt tatctcatat taatgttggt
     8521 acgggtgtag attgttccat acgtgaaatg gctgaaacaa tggcctctgt ggtgggttat
     8581 caaggtaaaa ttgtttttga tgttactaaa cctgatggca ctccgcgtaa acttatggac
     8641 gttacccggc tcaaaaactt gggctggcaa tatcaatata atttacatga aggcttatca
     8701 ttaacatata aatggtttat tgagaatatt aattcttttc ggggatagtt atgaataaga
     8761 gattggaacg tgagctattt aaaacaatag ttgagcatac tcctctaatc tcgattgatc
     8821 tcataattag aaacgataaa ggagaggcgc tgctggggca gcgcctgaat cgcccagcac
     8881 aaaattattg gtttgtgcca ggagggcgaa tttataagga tgagtcattc gagattgcat
     8941 ttaagcggat aacatttgaa gaggtgggcg ttcaaattag tcttgatgat gccttatttc
     9001 taggggtgta tgaacatttt tacaatgata atttttctga agcagaattt tctacacact
     9061 atgtagtgca tggatatgaa atccaactta aacctcagca acttcaccta ccaacggtcc
     9121 agcataattc ctacaagtgg tttgatgtag taacgttgct taatagcact acagttcatc
     9181 aatataccaa aaattatttt ataccaaggt aatagatatg ctacttcccg ttgtcatggc
     9241 cggtggttct ggtaccagat tatggcctct ttcacgtaca ctttatccga aacaatttct
     9301 atctttaaat agtcgtttaa ccatgttgca agagacattg cggcggcttg acaaggtaga
     9361 acataaaccc gctttggtca tttgtaacga atcacatcgc tttatcgttg ctgaacaatt
     9421 gcgtaaagag ggtttaaagc atagcggtat tttgcttgag cctgttggcc gtaatactgc
     9481 gcctgctgta gcactcgcag cacttcaggc tatggtaact ggagatgatc ctattctgtt
     9541 ggttcttgct gctgatcatg aaattcagaa tgaggataat ttcattgatg cagttcttgc
     9601 agccaagaat tttgcagaac agggtaagct tgttacgttt ggtattgttc caacatcccc
     9661 agagactggc tatggttaca ttaagtcagg tgagtatctg gatggaaaag gttacaaagt
     9721 tgcggctttt gttgaaaaac cagagcttca cgtagctcag cagtatatat cagatggcgg
     9781 ttatctttgg aatagtggga tgtttatgtt cagagcatct gtatttatca atgaactgaa
     9841 aaaattccga ccagatattt tagccagttg ccaacgctcc ttgtcctctt cgatacaaga
     9901 tttagatttt atccgtctgg ataatgcttc attttcttgc tgtcctgaag agtctattga
     9961 ctatgcagtt atggaaaaaa cagccgaagc tgtcgtcgtt cccttaaatg cacaatggag
    10021 tgatgttggg tcatggtctg cattgtggga aataagttca aaagatcaaa gcggtaatgc
    10081 cattcgtggt gatgtattgg ttgaagatgc tacagatagt tatctttatt cgcagcatag
    10141 acttattggt gctgtgggca taaaggattt ggttgttgtt gaaacgaaag atgccgtatt
    10201 agttgctcat aaagataaag ttcagcaagt taaaaacatc gtcgctcaac ttaaaaagaa
    10261 taatcgcaca gaatatttac agcatcggga aatatttcgg ccttggggca gtcatgatac
    10321 tatagctgaa gggccgcgct ttcaagtgaa acatgtgatt gtattacctg gccacattac
    10381 cgctaagcag attcattacc atcgtactga gcattggatt gttgtctcgg ggacagctaa
    10441 agtccatctt gaagataaga cttaccttgt ttctgaaaat gaatcaacat atatacctgt
    10501 tggtgttcca catgccattg aaaatcctgg caagatcccg cttgaaataa tcgaggttag
    10561 atcaggagtc tacctggaag aggatgatgt tataagagtt tcttcttctg gagtaggata
    10621 ctaatgcgaa tttctattat cacagctact tataatagtg aaaaaactct ccttgataca
    10681 ttactttctc tagaaaaaca aacgtatcca gatatcgaat atatagttgt agatggagca
    10741 tcgaacgata atacaattaa gctgatcaag agtaattgta caaaagtttc aaaaatcatt
    10801 tgcgaacccg ataagggcat ttatgatgct ctgaataaag gtattcaagc agcttcgggt
    10861 gatgttattg gttttttaca ttctgatgat ttattagctt atgatgatgc tattgcagat
    10921 atagcaaaaa catttgaaat tacagggtgt gatgctattt atggcgattt ggagtatgtt
    10981 gcccaaaatg atacgactaa acgtattaga ttatggaaaa gtggttcatt tagtcgtttc
    11041 aagatgaaag tgggttggat gccgccacac ccatcatttt atatgaaacg caaatgttat
    11101 aggcagtttg gttgtttttc tttagattat cgaatatctg ctgattatga ctcattatta
    11161 cgatatattt taaaacaacg tatttcaaca gaatatttac caaaagtctt agtgaagatg
    11221 cgtgttggtg gaattagcaa tcgttcagta tcttcaatgg taaagaagtc aatggaagac
    11281 attcgtgtta tgaaccagaa tggtattatc tggccaatag ctttagtgta taaaaatata
    11341 tccaaacttc ctcaatttat taaaaagtaa tcatcatgtt aaatgttaaa aaaatcatta
    11401 acgatagtaa tattgcgttc ggtactagtg gtgctcgcgg tttagtgatc gatttttccc
    11461 atgatgtttg tgctgcgttc actcatgcgt ttctttctgt tattgatgac aaatacaatt
    11521 ttaataaagt tgccttagca attgataacc gcccaagcag ttacgaaatc gctcaggcat
    11581 gcgccttcgc tatcaaacaa catgggttca ctgtcgaata tcatggtgta attccgactc
    11641 ccgcattagc tcattattcg atgcagaaaa acattccctg cataatggtc acagggagtc
    11701 atatcccttt tgatcgtaat ggcttaaaat tctacagacc tgatggtgaa atcactaaag
    11761 aggatgagct cgcaattata aatagtgagt atacattttc gcctgtaggt gtattacctc
    11821 atcttgaaac aagcactgaa ggtgcagact actaccttga gcgttatgtt tctcttttta
    11881 atcctgatat tttaaagggg aaaagaatag ggctatatga acattctagt gcaggacgcg
    11941 atttatatgc tcctgttttt aatcaattgg gtgcagaggt catttcctta ggtagaagtg
    12001 atgagttcgt tcctattgat acggaagcag taagcgatga agatcgcata cttgcaagag
    12061 agtggtctaa aaaatataat cttgatgcta ttttctcaac agatggcgat ggtgatcgtc
    12121 ctttagttgc cgatgaaaat ggtgaatggc taagaggcga tattctggga ttacttactg
    12181 ctattgaact taatatcaag gcgttggcta ttccagtgag ttgtaataca gcaattgaac
    12241 agtctaataa atttgcaagt atacaacgaa cgaaaatagg ttctccttat gtaattgcag
    12301 cgtttacaga tcttgctaag caatttaatt cagtcgctgg ttttgaagct aatggtggct
    12361 ttctccttgc ctccgattta caaattaatg gcaaggaatt aaaatcgtta cctacacgag
    12421 atgctgtgtt accagcatta atgctcttaa tagcttctcg caatagtact atttctcaac
    12481 tgattaataa tcttcctcag cgattcactt ggtcagatag agttaaaaac ttcccttcag
    12541 attcaagtca acaaattata aaaaatgcca tatcgtcacc caataatttc tttaatagtt
    12601 taggttatga atcattatcc tgttccgcta ttgatgaaac ggatggtgca agatttactt
    12661 taaataatgg tgatattata catctccgtc cttccggtaa tgctccagaa ctccgttgtt
    12721 atgctgaggc cagtgatgaa aatcaggcta agcaatatgt tacgaatgtg ctgggaaata
    12781 ttacctcttt gatttcttga tgttataggt ttatctacgc ttatacgtgt gcgtaggttt
    12841 gattacacgt agatgccggt atacagaatt gaagaacggt atttgttgca ttaatgaaat
    12901 tcagcactac acacattcgt gcaacttgag ataacatctc aatcatattc aagtcgcgca
    12961 tacatcgcgg tgaacacccc ctgacaggag taaacaatgt caaagcaaca gattggtgtc
    13021 gtcggtatgg cagtg
//

</pre>