LOCUS       KJ755551                8462 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain 14097 serotype O153:K-:H7 O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ755551
VERSION     KJ755551.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8462)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Baranzoni,G.M.,
            Liu,Y. and Debroy,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: Average 50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="14097"
                     /serotype="O153:K-:H7"
                     /db_xref="taxon:562"
     misc_feature    1..8462
                     /note="O-antigen gene cluster"
     gene            78..866
                     /gene="wbbD"
     CDS             78..866
                     /gene="wbbD"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-Gal:alpha-D-GlcNAc-diphosphoundecaprenol
                     beta-1,3-galactosyltransferase"
                     /protein_id="AJE24461.1"
                     /translation="MSVYINDKFNEFKEAFDSIINQTYSNFVFFIYADGKINPDIESF
                     LQNYNSQHVIFIRSEKNKGLAYALNKLISVALVNNEFDFIARMDADDISLEDRFQRQI
                     DFFMANPELDVVGGLCQEFGGESAREKCKIYFTDEAIKNNAFKKCPFVHPTVMFKSSV
                     FNNGMRYPENQPLTEDMCLWLELISANKKFANIDSVLLKYRINEQTLKRRRGLNKAVT
                     ELSTRLYYAKKLKMLSVRNLLYILIHFGVRVLPSNIFKLVHKLN"
     CDS             868..2634
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="AJE24462.1"
                     /translation="MNKTVYTYDVWDTILKRHCLPSYTLEVSLYWLLLYLGKPEKHKD
                     VYKKVKALESDFFNRKEEYFIEELIVGILIENNLMNGFCNNSVTAAWEQIYYFVERNC
                     TYKNKEVIELIGSDYGDKLFISDFYTDSSFIKKLLHHHQVSVLDGVTSAEYGVSKHMG
                     SLYEKIELLKQRKWIHTGDNELADIKMAKRAGAQVRLIKSKKKKINKLKIKNDYERLA
                     FIILSFSLFILKTSRKKKCNKIYFFTREGVFFKKNFDLLLNQIPNIFPSITTEILPIS
                     RVASLALKFNESDEFLGFNDALTQYGYGVSTFLSFFHLDDCYSELTTKYHDVNDLLSH
                     RNDPLVKQLIKSIEDKKKRTENFLTSIEFDSEPSIIVDIGWRGSIQDNLMSRKNNYIQ
                     HGCYLGLFDFYPGQSGQNKSSVIFDNNRFRQKWTMKGVAFMETVFNALDGSVVDYVNN
                     KPKRKENYAEIENSKHLIVMQDAIIEEFMNLSIMLKEGKLNINDIERLAIESYKKIVT
                     NPSKEIADFYVGSIQNESFGLNDFIDKEFNISAVDVFRSFFSKSKRNEIRIKLHRNGW
                     RESILKSSKVSLSAKLCSLLIR"
     gene            2638..3510
                     /gene="epsH"
     CDS             2638..3510
                     /gene="epsH"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="AJE24463.1"
                     /translation="MNISVIICCYNSENRIVNTLFHLSQCVSLSKGLIDEIILVDNNS
                     SDETVKTAEDFWRNTGEEQIFKIVFESIPGLVHARRRGLDESKNEIVVFCDDDNWLVP
                     SYFTKLEKYFENKQVGAVGGRGIPIANTVIPEWFFENLDAYACSAQTSNMLYGASLAI
                     RSSILKKFYTDALSVKLKGRTKDSLDSGEDNLLCNFVKKQGYTLIPDNTPFCHFMESK
                     RLDVDYLYKLAESFGKATASMKNNSFTTDYVYLTAKMLLAISRIFSKKNKKIYLKREI
                     SFLRGYLRFRKESK"
     gene            3522..4688
                     /gene="wzy"
     CDS             3522..4688
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AJE24464.1"
                     /translation="MLVIAWYSIFLLISATGMTGLNKLSTEAILCYIAFLFCFFFGMM
                     LGGVSLPNGMKMLQQEQKIKKRHASAIVLIYCFFIFYSLIALLNIYYFNKVDLSSYRQ
                     AFFDGSRDNVKYFYGVFGFYFYYLLSLLVFALVPYTIITNSKKTISLCFFALLLYDII
                     FLSRTGVYYFILSYIVSSILLKKGIKKIIIVGMVALLFSLIMSYTRDMKSDIIGTLFS
                     SIINYHIAPFVLFDENIISNHLIKYNGAGLASLGIYNIIFYPIDSSIMSSINDFRAQL
                     NEFYDLGVNRYLPYNAYYTSLGGVYIDSGLIGSMMVSFMTGFIIVGIEKSAFKSTKFL
                     VSSVFFTTLCMESIFSPITLNIFAFTIIVYLVVLMVLKYENSDDNTIFKKYRAK"
     gene            4651..5664
                     /gene="pglJ"
     CDS             4651..5664
                     /gene="pglJ"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylgalactosamine-N,
                     N'-diacetylbacillosaminyl-diphospho-undecaprenol
                     4-alpha-N-acetylgalactosaminyltransferase"
                     /protein_id="AJE24465.1"
                     /translation="MIIPSLKNTAPNNIALSICEGARKHGVNIDIYYLDEIVELDIDE
                     SELNINRLNDRTILNSYDIVHSHGLRPDVINCKTSKNVINVSTLHSFILDDLKNKYGI
                     KGYLIAFAWLKLLKRFDSCIAISEATKRFYVKHGLKCHHIYNGIDFPDIELYQDNSRL
                     NNGKINIVSIAHLEKIKGLEQLLYLAAEREEFHVHIIGEGTYRGKLTNIIDKFDLSQR
                     VTLHGYISNASSMLGKFDVYVQPSKSEGFGIAVIEALVNKIPTVCSDIEVFRELFGSG
                     EVEFFKLDDINSLYDAISSALTKTDMSRDASATAISQKFSSEVMSLNYLSWYKKLYEE
                     RNL"
     gene            5645..6823
                     /gene="wzx"
     CDS             5645..6823
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AJE24466.1"
                     /translation="MKKEIYKYGTIILCYGINFILPILFFKEISLILSPIVLSSFFFV
                     LALLMYCQFIVEYGLQISLLRKLNENKSDLSRLLSTILGLKVILFVLCAVFIYCILLY
                     NNEVIFFILVFILLGNVFSCQFLYQVVDQLHFFYVLNSLVKLIFIPLIFINDNYIYLM
                     ICYSCFNILPNLAALAYFLYKNKVKVVKVPACEIYNLSKECFSYFISNISITLYTNFY
                     QILLGIISPAYLAAYALSDKIIRGIVSGNYVFIQIVQVQYLNDESKFINGTWKTIVSL
                     LLLGGMEFLFIFFGADFFSRYLFPTISLLPIFLKLMSLLIVVILLSNFFAMVYLPCSG
                     NTLFLSRVLIFVSIVSVVVAPILIWLYAGYGAVISAIFSELLVLILCYGLYLKLKHKL
                     "
     gene            6846..7286
                     /gene="mdoB"
     CDS             6846..7286
                     /gene="mdoB"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerol transferase I"
                     /protein_id="AJE24467.1"
                     /translation="MNLLIVFCFYISVLRSNKISVFKLITITTVLIVYVFWLIADSFT
                     GVGINDSVYFHMLMPHEGASVENLTYYGFLIVSLVISIFLIAYVSWKKKHNRGLSKNY
                     SFYVLFAIVLVTPFMRNFLGSINNLISEQFSTEDISKEYVINSR"
     CDS             7531..8034
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 transposase"
                     /protein_id="AJE24468.1"
                     /translation="MPGNSPHYGRWPQHDFTSLKKLRPQSVTSRIQPGSDVIVCAEMD
                     EQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDG
                     WPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHY
                     LNIKHYQ"
ORIGIN      
        1 cactggtagc tgttaagcca ggggcggtag cgtggattga tggaatgaaa aataagtgcc
       61 cacgaattgc agtaataatg tcggtttata ttaatgataa atttaatgag tttaaagagg
      121 cttttgacag tattattaat caaacatact ctaactttgt tttctttatt tatgccgatg
      181 gcaagataaa tccagatatt gaaagttttt tgcaaaatta taattcgcaa catgtgattt
      241 tcattagaag tgaaaaaaat aagggattag cttatgcttt gaataaatta atatcagtgg
      301 cgctagttaa taatgaattt gattttattg ccagaatgga tgctgatgat ataagtttag
      361 aagatagatt tcaacgacaa atagattttt ttatggctaa ccctgagtta gatgttgttg
      421 gtgggttgtg tcaagagttt ggtggtgagt cagcaagaga aaaatgcaag atctatttta
      481 cagatgaagc cattaaaaac aatgccttta aaaaatgtcc tttcgttcat ccaaccgtga
      541 tgtttaaatc gtctgtattt aataacggga tgcgataccc tgaaaatcag ccactgacag
      601 aagatatgtg cttatggctc gagttaatat ccgcgaataa aaagtttgca aatattgact
      661 ccgttcttct aaagtacaga attaatgagc agacgctaaa aagaagaaga ggtttgaata
      721 aggctgtaac cgaattatcc acaagattgt attacgccaa gaaacttaaa atgcttagcg
      781 tacggaattt gttatatata ttaatccatt ttggtgtcag agtccttcct tcaaatatat
      841 ttaaacttgt acataagtta aattaaaatg aataaaactg tttatacata tgatgtatgg
      901 gatactattt taaaacgtca ttgtttacca tcatatacgt tagaggtttc gttgtactgg
      961 ctacttttgt atcttggaaa acctgagaaa cataaggatg tgtataaaaa agtaaaagca
     1021 ctggagagtg atttttttaa tcgaaaagaa gaatatttta ttgaagagct tattgttggg
     1081 atattaattg aaaataattt aatgaatgga ttttgcaata actctgtgac tgctgcttgg
     1141 gaacaaatat attatttcgt agagagaaat tgtacatata aaaataaaga ggttatagag
     1201 ttaattggga gtgactatgg ggacaaatta tttatttctg atttttatac tgactccagt
     1261 ttcataaaga aattgctaca ccaccaccaa gtatcggttt tagatggtgt gacgtcagct
     1321 gagtatggcg tcagtaagca tatgggttca ttgtatgaaa aaatagaact attgaagcaa
     1381 aggaaatgga tccatactgg agataatgaa ttagctgata taaaaatggc aaagcgtgca
     1441 ggtgcccaag ttagacttat aaaatccaaa aagaagaaaa taaataagtt aaaaataaaa
     1501 aatgactatg agcggttagc atttattatt ttgtccttta gcctgtttat attaaaaact
     1561 tccagaaaga aaaagtgcaa taagatctac ttttttacaa gggagggggt ttttttcaaa
     1621 aaaaactttg atcttctact aaaccaaata cctaacatat tcccatctat aactacggaa
     1681 atattgccaa tcagtcgagt agccagctta gcattgaagt ttaatgagtc tgatgaattt
     1741 ttaggattta atgatgcatt aacccagtat ggctatggag tgtctacgtt tcttagtttc
     1801 tttcatcttg atgattgtta ctccgagctc acaacaaagt atcatgatgt aaatgattta
     1861 ttatctcaca gaaatgatcc tttggtaaaa caactaataa aaagtataga ggataaaaaa
     1921 aaacgaacag aaaattttct tacatccatt gagttcgatt ctgagccaag tatcatcgtg
     1981 gatattggtt ggcggggtag catccaagat aatttaatgt cgcggaaaaa taattatatt
     2041 caacatggat gttatctcgg attgtttgat ttttacccag gccagtcagg tcagaataaa
     2101 tcatctgtaa tttttgataa caacagattt agacaaaaat ggaccatgaa aggtgtagct
     2161 tttatggaga cggttttcaa tgctttggac ggaagtgttg tcgattatgt aaacaataaa
     2221 ccgaaaagaa aagaaaatta cgctgagata gaaaattcaa aacatttaat agttatgcaa
     2281 gatgctatca ttgaggaatt tatgaatcta tctattatgt taaaagaggg aaagttaaat
     2341 atcaatgata ttgaaagatt agctattgaa agttacaaaa aaatagtaac taacccatct
     2401 aaagaaatag ctgattttta tgttggcagt atccaaaatg aaagcttcgg tttgaatgat
     2461 tttatcgaca aagaatttaa cattagcgca gtagatgttt tccgaagttt tttttctaaa
     2521 agtaaaagaa atgaaataag aataaaattg catagaaatg gatggcgtga gtcgatactt
     2581 aaatcctcta aagtatcgct gagcgcaaaa ttatgttcac ttttaattag ataaaatatg
     2641 aatatttcag taataatttg ttgctataac tcagaaaata gaatcgtaaa cacccttttt
     2701 catctttcac aatgtgtatc tctatccaag gggttaattg acgagattat tcttgttgac
     2761 aataactcat ctgatgaaac tgttaaaaca gctgaggatt tttggcgtaa tacgggggag
     2821 gagcaaatct ttaaaattgt ttttgaatcg attccgggcc ttgttcatgc tagacggcga
     2881 gggctggacg agtccaaaaa tgagattgtt gttttttgtg atgatgataa ttggctggta
     2941 ccatcttatt ttacaaaact agagaaatat tttgaaaaca aacaagtagg cgctgtcggg
     3001 ggacgcggta ttcctattgc taataccgtt attcctgagt ggttttttga gaatctggat
     3061 gcatatgcat gttctgcaca aacttctaac atgttgtatg gtgctagctt ggctattcgg
     3121 tctagtatct taaaaaaatt ttatacggat gctttatcag taaagctaaa gggtagaaca
     3181 aaagacagtc tggattctgg ggaagataac ttattgtgta attttgtcaa aaaacaagga
     3241 tacactctga tacctgacaa tacaccattt tgccatttca tggaaagcaa gcgattggat
     3301 gtcgattatc tatataaact ggctgaatca tttggaaaag caacggcatc aatgaaaaac
     3361 aattccttca ccaccgatta tgtatatttg acagcaaaaa tgttattagc tatatctagg
     3421 atcttctcta aaaaaaataa aaagatatat ttaaaacgtg agatttcctt tctcagaggt
     3481 tatttgaggt ttagaaagga atcgaagtaa tgagaggggg aatgcttgta attgcgtggt
     3541 attctatttt tctgttgatt tcagctacgg gcatgaccgg tctaaataag ctgagtactg
     3601 aagctattct gtgctatatt gcatttttat tctgtttttt ctttggcatg atgttaggtg
     3661 gtgtttcgct cccaaacgga atgaaaatgt tgcaacagga gcaaaagata aagaaaaggc
     3721 atgcttctgc catagttcta atttattgct tttttatttt ttattcttta attgctcttt
     3781 taaatatata ttatttcaat aaggttgatc tatcatctta tagacaagca ttttttgatg
     3841 gttcaagaga taatgttaaa tacttctatg gcgtgtttgg cttttacttc tattatttac
     3901 ttagcctgtt agtattcgcc ttagttcctt atactattat aacgaacagc aaaaagacta
     3961 tttcattatg tttctttgcc ttgctgctat acgatattat tttcctttca agaactggtg
     4021 tttattactt tatattatct tatatagtat cgagtattct tctcaagaaa ggaatcaaga
     4081 aaattataat tgtagggatg gttgccttat tattctcatt gataatgagt tacacaaggg
     4141 atatgaagag tgacatcatt ggtacattat ttagctcaat tataaattat catatagctc
     4201 cgtttgtctt gtttgatgaa aatataatat caaatcattt gattaaatat aatggggctg
     4261 gcttggcatc gttggggata tataatatca ttttttatcc cattgattcg tctatcatgt
     4321 catcaataaa cgattttaga gcacagttga atgaattcta tgatttggga gtaaatcgtt
     4381 atttgccata taatgcctat tatacttctt tgggtggggt atatatagat agcggcctga
     4441 ttggtagcat gatggttagt tttatgactg gttttattat tgtcggaatc gagaagagtg
     4501 cttttaaatc aactaagttc ttagtctctt ctgtgttttt tactacatta tgtatggaat
     4561 ctatattttc cccaataaca ctaaatattt ttgcctttac aataattgtc tacttagttg
     4621 ttttgatggt ccttaaatat gaaaatagcg atgataatac catctttaaa aaataccgcg
     4681 ccaaataata tagccctttc aatatgtgaa ggggcaagga agcatggtgt caatatagat
     4741 atttattatc ttgatgaaat tgtagagttg gatatagacg agtcagaact gaatataaat
     4801 aggctaaatg atagaaccat tttaaacagt tatgatattg ttcactctca tggcttaaga
     4861 cccgatgtta ttaattgtaa gaccagcaaa aatgtcatta atgtaagtac gcttcatagt
     4921 tttattttag atgatcttaa gaataaatat ggaataaaag gatatctgat cgcttttgct
     4981 tggcttaaat tattaaagcg atttgattca tgtattgcaa ttagtgaggc gactaaacgg
     5041 ttttatgtta aacatggtct taaatgccac catatatata atggaatcga ctttcctgac
     5101 atcgaattat atcaagataa ctcaaggcta aataacggca aaataaatat agtatcgatc
     5161 gcgcatctgg aaaaaataaa aggcttagag caactcctat atctagccgc ggagcgagaa
     5221 gagttccatg ttcatataat tggtgaagga acttaccgtg ggaaattaac aaatattatt
     5281 gataagtttg atttatctca gcgtgtaact ttgcatggat atatatcaaa tgcgagttct
     5341 atgttgggga aatttgatgt ttatgtgcaa ccatctaaaa gtgaggggtt tggcattgca
     5401 gtcattgaag ctcttgtaaa taaaatccca acagtttgct cagacattga agtgttcaga
     5461 gagttatttg gctcaggaga agttgaattt ttcaagttag acgatattaa ttcactttac
     5521 gatgcaattt caagtgcatt aaccaagaca gatatgtctc gagatgcaag tgccacagcg
     5581 ataagccaaa aattttcttc agaagtgatg tcattgaact atttaagttg gtataaaaaa
     5641 ttatatgaag aaagaaattt ataaatatgg cacaataata ttatgctatg gaattaactt
     5701 tatactacct attcttttct ttaaagaaat aagtttaata ttatctccaa tagtattatc
     5761 atcatttttc tttgttttgg cattgctaat gtattgtcaa ttcattgttg aatacgggtt
     5821 gcaaatatca ttattaagaa agttaaatga aaataagagt gatttaagtc ggcttttatc
     5881 tactattctt ggactaaaag taatactatt cgtcctatgc gcagtattca tttattgtat
     5941 tttgctatat aataatgaag ttatattttt tatacttgtt ttcattctgc taggaaacgt
     6001 attctcatgc cagttcctct atcaagtagt tgatcagctc catttttttt atgtattaaa
     6061 ttcgcttgtt aagctaattt tcatacccct catttttatc aatgataatt atatctatct
     6121 gatgatatgt tatagttgtt tcaatatcct acctaactta gctgcacttg cttatttttt
     6181 atataagaat aaagttaagg ttgtaaaagt tcctgcttgt gaaatttata atttatctaa
     6241 ggaatgtttt agctatttta tctcgaatat ttcgataaca ttatacacta atttttatca
     6301 gatcttatta ggcattatat cgcccgctta tctggccgcg tatgcattaa gtgacaaaat
     6361 tattaggggg atcgtgtcag ggaattatgt gtttatacag atagtacaag tacaatactt
     6421 aaatgatgaa tcaaagttca tcaatggtac atggaaaacg attgtttcat tattattact
     6481 gggggggatg gagtttttat ttattttctt tggagcggat tttttctctc gttatttatt
     6541 ccctacaata tctttactgc caatttttct aaaactaatg tcattgctaa ttgttgttat
     6601 cttgttgagt aacttttttg caatggttta tcttccgtgt tcaggcaata ctttattcct
     6661 ctcacgtgtt ctgatttttg tttctattgt gtccgtagta gttgccccga tactaatatg
     6721 gctctatgca ggttatggtg cagtcatttc cgctatattt tcagagttat tagttctgat
     6781 tttatgctat ggtttatatt tgaaattaaa acacaaactc taactattaa cgtagaataa
     6841 ttaaaatgaa tttattgatt gttttttgct tttatatttc ggttctgcgt agtaataaaa
     6901 taagtgtatt taaattaatc actatcacaa ctgtcttgat tgtatatgta ttctggctaa
     6961 tagccgacag ttttactggc gttgggataa atgattcagt ttatttccat atgttaatgc
     7021 cgcatgaggg ggcatcggtt gaaaatttaa catattatgg atttctgatt gtaagccttg
     7081 ttatttcaat tttcttgatt gcttatgttt cttggaaaaa gaagcataat agaggattaa
     7141 gtaaaaatta ctctttttac gtactcttcg ctattgtatt agttactcca tttatgcgta
     7201 atttcctggg gtcaattaac aatttaattt ccgaacagtt ttctactgaa gatataagta
     7261 aagagtatgt aatcaatagt aggtgatgct gccaacttac tgatttagtg tatgatggtg
     7321 tttttgaggt gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga
     7381 cggggtggtg cgtaacggca aaagcaccgc cggacatcag cgctatctct gctctcactg
     7441 ccgtaaaaca tggcaactgc agttcactta caccgcttct caacccggta cgcaccagaa
     7501 aatcattgat atggccatga atggcgttgg atgccgggca acagcccgca ttatgggcgt
     7561 tggcctcaac acgattttac gtcacttaaa aaactcaggc cgcagtcggt aacctcgcgc
     7621 atacagccgg gcagtgacgt catcgtctgc gcggaaatgg acgaacagtg gggctatgtc
     7681 ggggctaaat cgcgccagcg ctggctgttt tacgcgtatg acaggctccg gaagacggtt
     7741 gttgcgcacg tattcggtga acgcactatg gcgacgctgg ggcgtcttat gagcctgctg
     7801 tcaccctttg acgtggtgat atggatgacg gatggctggc cgctgtatga atcccgcctg
     7861 aagggaaagc tgcacgtaat cagcaagcga tatacgcagc gaattgagcg gcataacctg
     7921 aatctgaggc agcacctggc acggctggga cggaagtcgc tgtcgttctc aaaatcggtg
     7981 gagctgcatg acaaagtcat cgggcattat ctgaacataa aacactatca ataagttgga
     8041 gtcattaccg gttacgagta gtggcatctg gatttgatgt gtgtaacatt aatgagtcgt
     8101 taatattagc tagagttggt gataatatgt tggctaggcg aaaagggctg aggtatataa
     8161 aaagtgaatt ccaactttat cggctaaaag ttaaacttgg tttgcagtca catatatctg
     8221 gattctgcat tttgttagta agaagcattc cgcgattact ccctctaaag gtcttaaaaa
     8281 ttatttacca atatattcgg aagtaattat ataataattc tgaagattgc actcttattt
     8341 tttcttagtt gtatctttgt atgttaacat tacgtttaaa ccgaaccgct aagacggtaa
     8401 tttcgtgaca ggagtaaaca atgtcaaagc aacagatcgg cgtagtcggt atggcagtga
     8461 tg
//