<pre>LOCUS       EU294168               13552 bp    DNA     linear   BCT 26-AUG-2008
DEFINITION  Escherichia coli serotype O150 O antigen gene cluster, complete
            sequence.
ACCESSION   EU294168
VERSION     EU294168.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13552)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Structure and genetics of Shigella O antigens
  JOURNAL   FEMS Microbiol. Rev. 32 (4), 627-653 (2008)
   PUBMED   18422615
REFERENCE   2  (bases 1 to 13552)
  AUTHORS   Liu,B., Knirel,Y.A., Feng,L., Perepelov,A.V., Senchenkova,S.N.,
            Wang,Q., Reeves,P.R. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2007) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, Tianjin
            300457, P. R. China
FEATURES             Location/Qualifiers
     source          1..13552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /serotype="O150"
                     /db_xref="taxon:562"
     misc_feature    1138..12109
                     /note="O antigen gene cluster"
     gene            1138..2223
                     /gene="rmlB"
     CDS             1138..2223
                     /gene="rmlB"
                     /note="dTDP-D-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ACA24793.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAVMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            2223..3122
                     /gene="rmlD"
     CDS             2223..3122
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-L-mannose-dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ACA24794.1"
                     /translation="MNILLFGKTGQVGWELQRALVPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPKFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQEEDATAPLNVYGETKLAGEKALQEHCVKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWYDYAALVFEEARKADIPLALNKLNAVPTTAYPTSARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            3180..4058
                     /gene="rmlA"
     CDS             3180..4058
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ACA24795.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLHYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            4063..4614
                     /gene="rmlC"
     CDS             4063..4614
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ACA24796.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSCKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKWSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFVYKTTNYYAPGYEGSIIWNDKILSIDWPEL
                     TLEYILSDKDLKAPILSESIENI"
     gene            4616..5599
                     /gene="wfgF"
     CDS             4616..5599
                     /gene="wfgF"
                     /note="hydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfgF"
                     /protein_id="ACA24797.1"
                     /translation="MFISRVKRKIQSLISLELNFLHDYWRFKKYYTKGSQASKDKQKL
                     ESWILQDKHRIEKAFSLPKPKLGFGKDVIPRLIDNLVNYSSKFGNDQVYYIGLGALQS
                     YKRFHDEQKFLLPEFYSKNICKIRNDDFLDPRCNVAGYFKKDDSLVTGKITVESLMNE
                     RRSCRHFDVDESLNIGDKLLKDVTKLSITAPSVCNRQHWRIHYFSGELKNKILSYQNG
                     NSGFTENIPYVAVITSDLRAFYSTDERNQPYTDGGIFAMNFMYALQHYGLASCPLNWC
                     NSSHIENEFRETKLIPDYEVVVLVVAFGYPNKDALYAKSPRLSLNNLYTIN"
     gene            5632..7134
                     /gene="wzx"
     CDS             5632..7134
                     /gene="wzx"
                     /note="O antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ACA24798.1"
                     /translation="MKKQIIKNISANFTNLIVSIFIGLLLTPLLVKSLGIEVYGVLPI
                     ALFLTFYMGVIAQALTASVNRFLIEDFIKGAYTEASVVFSTSFILILGYLSVISIGFI
                     YPILHISDFFNIPPGYEHDAVLLFSCVIFSFVIAMISSSLSVSMYAKNRIDLMQYGNI
                     LRNITKVLIIIMFVYLSEINLTKIGISIVLSELFYLIYVIITWKILTPQLSLQIGFFR
                     KDKVKRLTSFSGWILFDQIGSILFLKTDLILVNSLSGSKANGEYSIATQFSDLLRSLA
                     GLVAGSLGPMMVILYEEKKLEELTSLTITFVKFLSLTIAIPIIILCVYSKEILELWLG
                     SGFVFLSPLICIVTLPLIINLGTLPILSINIAVKKIQVPALINFFLAIVGIIGSVVLF
                     KTTTLGYYAVAISYVITLTIKNSIFIPWYATIILNKPRYLFFKTHLITIIFSSLFFIF
                     IYILKSIMQITNLNLLFELILVGCFGLLSTFPFYSKEERRSFMLMLKRKV"
     gene            7137..8282
                     /gene="wfgG"
     CDS             7137..8282
                     /gene="wfgG"
                     /note="pyruvyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfgG"
                     /protein_id="ACA24799.1"
                     /translation="MDSTQKIGVLNFQYSDHNYGAVLQAAAIEQFLKSNGYNAEHIDY
                     ISSPEIKGNRVIIQLKCILKKLGLSDSVRKMLGKQVHLTPVVTNEKVFEEFRKNWLVR
                     TKCFRNFEELKKTDFNFKAVIVGSDQVWRPSQFTRFSDYKVYFLSFLPSQVKKISYAA
                     SFGIDKWEVSDANVTNEIKKYIQDFNAVSVREDSGVNICATIFNVDAEHVLYPTLLVG
                     SDFFNSIIAKEIRADAYYDAIVYYKLDIDQHFIDSVTILGRKKCKPVKNIYYNQVDGR
                     NEYYSVPEWLSNIKNANFVITDSFHCVCFCLLFQKEFLCCVNESRGLSRLQSLLGMLE
                     LTDRICSSKDDFDNKLNSLKPIDYSKVGDILNKHRDISKAFLLSNLS"
     gene            8307..9197
                     /gene="wfgH"
     CDS             8307..9197
                     /gene="wfgH"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfgH"
                     /protein_id="ACA24800.1"
                     /translation="MNANNRNAFPIIVMTRNEGADLQRCVDSILHTVSIDVHIYIIDN
                     NSDDLSHIQILANLEDLNRKNLTVIRNTRNLWILGVNNTLLKIKKNHDTKYFILTDGD
                     IDFHGFKEESCWLTYLINKMESNVIIGKLGISLDWSFLASKTEMKDIYRQEQNLYNER
                     RKIGDLYISAVDTTVAIYRWDWSIERKALFYPDHMRYLRPELYSCRTKRGLTVEHLGW
                     YRYLDNSLSTQSINSKVFCFTIVGGTVKPEVLKLASKPYQYFHILFSKPFAMLWYFRR
                     YFKLFIYFITKGRRGFDGQS"
     gene            9184..10098
                     /gene="wfgI"
     CDS             9184..10098
                     /gene="wfgI"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfgI"
                     /protein_id="ACA24801.1"
                     /translation="MDSHNNKLSKRIPSPALRCYAVIVTFNPDINKVRSLIDILQKNF
                     ITAVVVDNTIGAINYPFSCHVINLGDNLGIATAQNKGIEYCLTKQAEAIWFFDQDSVI
                     TSSFVERFLSTVHENPHDKIFAPVFWDEKKGFEYAITNIDSKGNRQKLLSSSFPSDFY
                     SSIVISSGCLIKSDLLMKIGPMLDFLFIDYVDTEWCLRAFYYGYKVHIIKNARMKHSI
                     GDNTISLIGFNVPIHSPLRRYYRIRNAFLLIRFKHIPKKLAFREVIFCCCHQLIIILT
                     QEKKLAYVKYFLRAVYDGLRNKTGKFVN"
     gene            10098..10994
                     /gene="wfgK"
     CDS             10098..10994
                     /gene="wfgK"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfgK"
                     /protein_id="ACA24802.1"
                     /translation="MSFVMKPKVLVLVAVYNGEKWLIEQLTSIINQKNVDIDVIINID
                     KSTDSSYKLCENFIADNVKILPYGAVFGGAGANFYHLIHESEFSGYDYIAFSDQDDIW
                     FETKIYDAINRMHDYDAYSSNVIAFWENGKQCLIEKSQPQTEFDYFFEAAGPGCTYIV
                     RQELALAFKYFVCKNYVAVNKVCLHDWLLYAFARERKYKWFIDPQPGMLYRQHSNNQV
                     GANASLLGAIKRIKKIKDSWYRNEVKKIVNLTSIDNNLISECLMRDRYLSSLKLAFIV
                     RKLRRRPRDQFAMFIALVLGWF"
     gene            11012..12109
                     /gene="wzy"
     CDS             11012..12109
                     /gene="wzy"
                     /note="O antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ACA24803.1"
                     /translation="MLILTNNIILLIFIIYTFLVFISALGVKLNSRGLDNFSCLITVL
                     TFVILIFARTGLGVDESTYLESYHYYVQGGEADFEYGFNILFWGVRLFGVTESSFNTI
                     FPLLILTALYFAVTVSVKNPFRSFVLIFTIFSSFFLDFSFNAYRQGLSFWFVLLAIES
                     HLSGNRWKFIVYCLIGLGFHWSAAVVLFMLFLTRFLSTRLAIFCNIFLFFLTLVAAMM
                     PLHVLSMLVTMIQYLPLNSSYLQKVIFYLTTIKSSFYDLNFFGRAPLIIYALILISLL
                     AIYRKLLPIVQFKLLILLLVYSVLLLEMSYSFRNYYWVLPFTPFIMAKILSCYSNKKK
                     VMVMYTMFSTGLWSLSIASFYSFPILSMIFQ"
ORIGIN      
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc acgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctcttc ttgaactgcg cgtgaagcgt
      121 caactactgg cggaagttca gtccatctgc ccgccgggcg tgaccattat gaacgtgcgt
      181 cagggcgaac ctttaggttt gggccactcc attttgtgtg cgcgacccgc cattggtgac
      241 aatccatttg tcgtggtgct gccagacgtt gtgatcgacg atgccagcgc cgacccgcta
      301 cgttacaacc ttgctgccat gattgcgcgc ttcaacgaaa cgggccgcag ccaggtgttg
      361 gcaaaacgta tgccgggtga cctctctgaa tactccgtca ttcagaccaa agaaccactg
      421 gatcgtgaag gtaaagtcag ccgcattgtt gaatttatcg aaaaaccgga tcagccgcag
      481 acgctggact cagacatcat ggccgtaggt cgctatgtgc tttctgccga tatttggccg
      541 gaactggaac gtactcagcc tggtgcatgg ggacgtattc agctgactga tgctattgcc
      601 gagctggcga aaaaacaatc cgttgatgtc atgctgatga ccggtgacag ttacgactgc
      661 ggcaaaaaaa tgggctatat gcaggcgttt gtgaagtatg gcctacgcaa cctgaaagaa
      721 ggggcgaagt tccgtaaagg tattgagaag ttgttaagcg aataatgaaa atctgaccgg
      781 atgtaacggt taacaagaaa attataacgg cagtgaagat ttgcagcaaa agtaatttgt
      841 tgcgaatatt cctgccgttg ttttatataa accatcagaa tagcaacgag ttagcagtag
      901 ggttttattc aaagttttcc aggattttcc ttgtttccag agcggattgg taagacaatt
      961 agcgtttgaa tttttcgggt ttagcgcgag tgggtaacgc tcgtcacatc ataggcatgc
     1021 atgcagtgct ctggtagctg taaagccagg ggcggtagcg tgcattaata cctctattaa
     1081 tcaaactgag agccgcttat ttcacagcat gctctgaagt aatatggaat aaattaagtg
     1141 aaaatacttg ttactggtgg cgcaggattt attggttctg ctgtagttcg tcacattata
     1201 aataatacgc aggatagtgt tgttaatgtc gataaattaa cgtacgccgg aaacctggaa
     1261 tcacttgctg atgtttctga ttccgaacgc tatgtttttg aacatgcgga tatttgcgat
     1321 gcagctgtaa tggcacggat ttttgctcag catcagccgg atgcagtgat gcacctggct
     1381 gctgaaagcc atgttgaccg ttcaattaca ggtcctgcgg catttattga aaccaatatt
     1441 gttggtacat atgtcctttt ggaagccgct cgcaattact ggtctgctct tgatagcgac
     1501 aagaaaaata gcttccgttt tcatcatatt tctactgacg aagtctatgg tgatttgcct
     1561 catcctgacg aggtaaataa tacagaagaa ttacccttat ttactgagac aacagcttac
     1621 gcgccaagca gcccttattc cgcatccaaa gcatccagcg atcatttagt ccgcgcgtgg
     1681 aaacgtacct atggtttacc gaccattgtg actaattgct ctaacaatta tggtccttat
     1741 catttcccgg aaaaattgat tccattggtt attctcaatg ctctggaagg taaagcatta
     1801 cctatttatg gtaaagggga tcaaattcgc gactggctgt atgttgaaga tcatgcgcgt
     1861 gcgttatata ccgtcgtaac cgaaggtaaa gcgggtgaaa cttataacat tggtgggcac
     1921 aacgaaaaga aaaacatcga tgtagtgctc actatttgtg atttgttgga tgagattgta
     1981 ccgaaagaga aatcttaccg cgagcaaatt acttatgttg ccgatcgtcc gggacacgat
     2041 cgccgttatg ccattgatgc tgagaagatt ggtcgcgaat tgggatggaa accacaggaa
     2101 acgtttgaga gcgggattcg gaagacagtg gaatggtacc tgtccaatac aaaatgggtt
     2161 gataatgtga aaagtggtgc ctatcaatcg tggattgaac agaactatga gggccgccag
     2221 taatgaatat cctccttttt ggcaaaacag ggcaggtagg ttgggaacta cagcgtgctc
     2281 tggtaccttt gggtaatttg attgctcttg atgttcactc cactgattac tgtggtgatt
     2341 ttagtaatcc tgaaggtgta gctgaaaccg taagaagcat tcggccggat attattgtca
     2401 acgcagccgc tcacaccgca gtagacaaag cagaatcaga accgaagttt gcacaattac
     2461 tgaacgcgac gagtgtcgaa gcgatcgcga aagcagccaa tgaagtcggc gcctgggtta
     2521 ttcactactc tactgactac gtatttccgg ggaccggtga aataccatgg caggaggagg
     2581 atgcaaccgc accgctaaat gtttacggtg aaaccaagtt agcgggagaa aaagcattac
     2641 aagagcattg tgtgaagcac cttattttcc ggaccagctg ggtctatgca ggtaaaggaa
     2701 ataacttcgc caaaacaatg ttacgtctgg caaaagagcg tgaagaattg gccgttatta
     2761 acgatcagtt tggtgcgcca acaggtgctg agctgctggc tgattgtaca gcacatgcca
     2821 ttcgtgtcgc attgaataaa ccggaagtcg caggcttgta ccatctggta gccagtggta
     2881 ccacaacctg gtacgattat gctgcgctgg tttttgaaga ggcgcgcaaa gcagatattc
     2941 cccttgcact caacaaactc aatgcggtac caacaacagc ctatcctaca tcagctcgtc
     3001 gtccacataa ctctcgcctt aatacagaaa aatttcagca gaattttgcg cttgttttgc
     3061 ctgactggca ggttggcgtg aaacgaatgc ttaacgaatt atttacgact acagcaattt
     3121 aatagttttt gcatcttgtt cgtgatgatg gagcaagatg aattaaaagg aatgatgaaa
     3181 tgaaaacgcg taaaggtatt attttagcgg gtggctctgg tactcgtctt tatcctgtga
     3241 ctatggctgt cagtaaacag ctattaccta tttatgataa gccgatgatc tattacccgc
     3301 tctctacact gatgttggcg ggtattcgcg atattttgat tatcagcacg ccacaggata
     3361 ctcctcgttt tcaacaactg ctgggtgatg ggagccagtg ggggctaaat cttcactaca
     3421 aagtgcaacc gagtccggat ggtcttgcgc aggcgtttat tatcggtgaa gagtttattg
     3481 gtggtgatga ttgtgctttg gtacttggtg ataatatctt ctacggtcac gacctgccta
     3541 agttaatgga tgccgctgtt aacaaagaaa gtggtgcaac ggtatttgcc tatcacgtta
     3601 atgatcctga acgctacggt gtcgttgagt ttgataaaaa cggtacggca ataagcctgg
     3661 aagaaaaacc gttacaacca aaaagtaatt atgcggtaac tgggctttat ttctatgata
     3721 acgacgttgt cgaaatggcg aaaaacctta agccttctgc ccgtggtgaa ctagaaatta
     3781 ccgatattaa ccgtatttat atggaacagg ggcgtttatc tgttgccatg atgggacgtg
     3841 gttatgcatg gttggacacg gggacacatc aaagtcttat tgaggcaagc aatttcatcg
     3901 cgacaataga agaacgacag gggctgaaag tttcctgccc ggaagaaatt gcttaccgta
     3961 aggggtttat cgatgctgag caggtgaaag tattagctga accgttgaag aaaaatgctt
     4021 atggccagta cctgctaaaa atgattaaag gttattaata aaatgaacgt aattaaaaca
     4081 gagattccag acgtattaat tttcgaaccg aaagtttttg gtgatgagcg tggtttcttt
     4141 atggaaagct ttaatcagaa agttttcgaa gaagctgtag gacgtaaggt tgaatttgtt
     4201 caggataacc attcgaagtc ttgtaaaggt gttttacgcg ggctgcatta tcagttagaa
     4261 ccttatgcgc aagggaaatt ggtacgttgc gttgttggtg aggtttttga tgttgcggtt
     4321 gatattcgta aatggtcacc tacctttggc aaatgggttg gggtgaattt atctgctgag
     4381 aataagcgcc agttgtggat ccctgaagga tttgcacatg gttttttggt gctaagtgag
     4441 acggcagaat ttgtttataa gacgacgaat tattatgcac ctgggtatga aggtagcatc
     4501 atttggaatg ataaaatact atctattgat tggcctgagt taacactgga gtatattctt
     4561 tccgataaag atctaaaggc cccaattcta agtgaatcta tagagaatat ttaaaatgtt
     4621 tattagtaga gtaaaacgta aaattcaatc tttgataagc cttgagttga attttttaca
     4681 cgattattgg aggtttaaaa aatattacac gaagggatct caagcttcga aagataagca
     4741 gaaacttgaa tcttggatat tgcaagataa acatcgaata gaaaaagctt tctctttacc
     4801 taagccaaaa cttggatttg gtaaagatgt aatacctcgt ctcattgaca atcttgttaa
     4861 ttatagcagt aaatttggaa atgatcaggt atattatatt ggcttaggtg cattacaatc
     4921 gtataagaga ttccacgacg aacaaaaatt tttattgcct gaattttatt caaagaatat
     4981 ttgtaaaata cggaatgatg attttctcga tccccgatgc aatgtcgctg gctattttaa
     5041 aaaagatgat tcattagtta caggtaagat aacagtagaa tcattgatga acgaacggcg
     5101 tagctgtcgg cattttgatg ttgatgaaag tcttaatatc ggagataaac ttttaaagga
     5161 tgttacaaaa ctatctatta cagccccttc tgtttgtaat agacaacatt ggcgcattca
     5221 ctatttttca ggtgaattaa agaataaaat tcttagttat caaaatggaa attcaggatt
     5281 tacagaaaac ataccttatg tagcagttat aacaagtgat cttagagcat tttattctac
     5341 tgacgaaaga aaccagccct atacagacgg tgggatcttt gccatgaatt ttatgtatgc
     5401 tctacaacac tatggtcttg cttcttgtcc attgaattgg tgtaattcat ctcacattga
     5461 gaatgagttt agagaaacta agttgattcc tgattacgaa gtagtagtac ttgtggttgc
     5521 ttttggctat ccaaataaag atgccttata tgctaaatca ccacgattgt ctctaaataa
     5581 cctttacaca attaattagg cgaaggccac ctttaaaggt ggctgacaat aatgaagaaa
     5641 caaatcatta aaaatatttc agccaatttt actaatttga ttgttagtat ttttattgga
     5701 ctattattga cgcctttact cgtcaaaagt ttaggcattg aagtatatgg agttctaccg
     5761 atagctttat ttttaacatt ttatatggga gtcattgctc aagcattaac tgcatctgta
     5821 aatagatttt taattgagga ttttattaaa ggagcatata ctgaggcaag tgtcgtattt
     5881 agtacatcgt ttattcttat tctaggatat ctatctgtta ttagtattgg atttatttat
     5941 ccaatattgc atataagtga tttttttaac ataccaccgg gatatgaaca tgatgcagtt
     6001 cttctattct cgtgtgtaat attttcgttt gttattgcta tgatcagttc atcattgtca
     6061 gtttctatgt atgcaaaaaa taggattgac ttgatgcaat atggaaacat tttaaggaat
     6121 attactaaag tcttaatcat tataatgttt gtttatttat ctgaaataaa tttaacaaaa
     6181 ataggaatat caattgtttt atctgagctg ttttatctaa tctatgtcat tataacttgg
     6241 aaaatattga caccacaact ttctctgcag ataggatttt tcagaaagga taaggtaaag
     6301 agattaacat ctttcagtgg ctggatattg tttgatcaaa ttgggtcaat tctttttctt
     6361 aaaacagatc tgattttagt taattctcta agtggtagta aagctaatgg tgaatattca
     6421 atagctacac aatttagtga tcttttgcgt tcacttgctg gtttggtagc tggtagttta
     6481 ggtcctatga tggtaatttt atatgaagaa aagaaattag aagaacttac ttcattgacc
     6541 attacatttg ttaaattcct aagcttgacg atagctattc ctattattat tctttgtgta
     6601 tactctaaag aaattcttga gctttggtta ggttcggggt ttgtatttct ttcaccatta
     6661 atatgtatcg taacgcttcc actgattatc aatttgggca cactgccaat attatcgatc
     6721 aatattgcag tcaaaaaaat tcaagtgcct gcactcataa atttctttct tgcaatcgtt
     6781 ggaattatag gctctgtagt attgttcaaa accactacat taggttatta tgctgtagct
     6841 ataagttatg tgattacatt aactattaag aactccattt tcataccatg gtatgcaaca
     6901 attatattaa ataaaccaag gtatcttttt tttaaaactc atctaattac tatcatcttc
     6961 tcttctctat tttttatttt tatatatata cttaaaagta taatgcagat tactaattta
     7021 aatttgcttt ttgaattgat attggttggt tgctttggac ttctatctac atttcctttc
     7081 tatagtaaag aggaaaggcg atcatttatg ttaatgctca agaggaaagt gtaataatgg
     7141 attcgactca gaaaataggc gttctgaatt tccagtattc ggatcataat tatggtgctg
     7201 ttttacaagc cgcagcaata gaacaatttc tgaaaagcaa tggatataat gctgaacata
     7261 tagactatat atctagtcca gagataaagg gtaatagggt aattatacaa ctaaaatgta
     7321 tattgaaaaa attgggacta tctgattctg taagaaaaat gcttggaaaa caagtccact
     7381 tgacacctgt ggtcacaaac gaaaaagtat ttgaggaatt tagaaaaaat tggttggtga
     7441 gaacgaagtg tttcagaaac tttgaagaat taaaaaaaac tgattttaat tttaaagcgg
     7501 ttatagttgg gagtgatcag gtttggcgac catctcaatt tactcggttt tccgattata
     7561 aggtatattt cttaagcttt ttaccttcgc aggttaagaa aatatcttat gcagcaagtt
     7621 ttggtattga taagtgggaa gttagtgatg ctaacgtcac gaacgaaata aaaaaatata
     7681 ttcaagattt caatgctgtt tcagtcagag aagatagtgg cgttaatatt tgtgcaacca
     7741 tttttaatgt cgacgctgaa catgttcttt atcctacttt actggttggt agtgactttt
     7801 ttaacagtat aatagctaag gaaatacgtg ctgatgctta ttacgatgct atagtttatt
     7861 acaaacttga tattgatcaa cattttatag attcagttac tatactaggt agaaagaaat
     7921 gcaagccagt aaaaaatatt tattacaacc aagtagatgg ccgaaatgag tattattctg
     7981 ttcctgaatg gttaagcaat attaagaatg ctaattttgt tatcacagat tcttttcatt
     8041 gtgtatgttt ttgtcttctt tttcaaaaag agtttttatg ctgtgtgaat gaaagtagag
     8101 gattatctag attgcaaagt ttacttggaa tgcttgagct tactgatcgt atttgctcgt
     8161 caaaagatga ttttgataat aagttaaatt cgttaaagcc aatagactat tctaaagtgg
     8221 gcgatatatt gaataagcat agagatattt ctaaggcgtt tttattaagt aatttatctt
     8281 gagtttgagt gtgggttaaa tatttaatga atgcaaataa tagaaatgca tttccgatca
     8341 ttgttatgac tcgcaacgaa ggcgccgact tacaacgttg cgttgattct atattacata
     8401 ctgtatctat agatgtacat atatatatca tagacaataa ttctgatgat ttatcacata
     8461 ttcagatcct tgctaacctt gaagacctga atcgtaaaaa tttaacagtt ataagaaata
     8521 caaggaattt atggatttta ggtgtaaata acacactatt gaaaatcaag aaaaatcatg
     8581 atactaaata ttttattctc actgatggtg atatagactt tcatggtttt aaagaagaaa
     8641 gttgttggtt aacatattta atcaataaaa tggaatccaa cgtgataatc ggtaagctcg
     8701 gaatttctct ggattggtca tttctggcct caaaaacgga gatgaaagat atttaccggc
     8761 aagaacaaaa tctctataat gaaagaagaa aaatcggtga tctttatata tctgccgttg
     8821 acacgacagt agccatttat cgttgggatt ggtctataga gcgtaaagct ttattttatc
     8881 ctgaccatat gaggtattta cgtccagaac tgtactcttg ccgtactaaa cgtggtttaa
     8941 cagtagagca tttaggatgg tatagatacc ttgataactc cctgtctacg caaagtataa
     9001 actcgaaagt tttttgtttt acaattgttg gtggtactgt taaacctgag gttttaaaac
     9061 tggctagtaa accctatcag tatttccata ttttgttttc aaaacctttc gctatgcttt
     9121 ggtatttcag acgatatttt aaacttttta tatattttat tactaaaggg agacgtggat
     9181 ttgatggaca gtcataataa taagttatca aaacgtattc cgtcacctgc actacgttgt
     9241 tatgcagtaa tagttacatt taaccctgat attaataagg ttagaagttt aatagatatt
     9301 ctacaaaaga attttatcac cgcagttgta gtagataata cgattggagc aataaattat
     9361 ccattctcat gtcatgttat taatttaggt gataatttag gtattgccac agcacaaaat
     9421 aaaggcatag aatattgttt gactaaacaa gctgaagcta tctggttttt tgatcaggac
     9481 agcgtaatca cttcttcatt tgtcgaaaga tttctatcta ctgtccatga aaatcctcat
     9541 gataagatct ttgctcctgt gttctgggat gagaagaaag ggtttgaata tgcaattaca
     9601 aatatagatt caaagggcaa cagacaaaag ttgttatcat catcttttcc atcggatttt
     9661 tatagttcga ttgttatttc atcagggtgc ttaataaaaa gtgatttgct gatgaaaatt
     9721 ggtccaatgc tagatttttt atttatagat tacgttgata cggaatggtg tttacgtgct
     9781 ttctattatg gctacaaagt tcacataatc aaaaatgcaa ggatgaaaca ttctataggt
     9841 gataacacta tttctttaat cggttttaat gtaccaattc actctcctct tcgacgttat
     9901 tatcgaataa gaaatgcatt tttattgatt aggtttaagc atatcccaaa aaaactagca
     9961 tttagagaag tgatattttg ttgctgccat caattaataa ttatcctgac ccaagaaaaa
    10021 aaactggcat atgttaagta ttttttacgg gctgtatatg atggccttag aaataaaact
    10081 gggaagtttg taaactaatg agttttgtca tgaagccaaa ggtattagtg ctagttgcag
    10141 tttataatgg tgaaaagtgg ttaatagagc aacttacatc tataataaat caaaaaaatg
    10201 ttgatattga tgtgattatt aatatcgata aatcaactga ttcttcatac aaattgtgtg
    10261 agaattttat tgcagataat gtcaagatac taccgtacgg agctgtattt ggcggtgcag
    10321 gtgccaattt ttatcatcta atccatgaat ctgaattttc tggttacgat tacattgcat
    10381 tttccgatca agacgatatt tggtttgaaa ctaaaatata tgatgccatc aataggatgc
    10441 atgattatga tgcatattca agtaatgtaa tagcattctg ggaaaatggc aaacaatgtc
    10501 taattgagaa atctcaacca cagacagaat ttgattattt ttttgaggca gcaggacccg
    10561 ggtgcactta tatagtgaga caggaactag cactcgcatt taaatatttc gtttgcaaga
    10621 attatgtggc tgtaaataaa gtttgtttac atgattggct gttatatgcg tttgccagag
    10681 aaagaaaata taaatggttt attgatcctc aacctggtat gttgtataga cagcattcga
    10741 ataatcaagt tggggcaaat gcttcattat taggtgctat caagcgtata aaaaaaatta
    10801 aagattcttg gtatagaaat gaggtaaaaa aaatagtcaa tttaacttcg attgataata
    10861 atttaataag tgaatgcctt atgagagata gatacttatc atctttaaaa ttggctttta
    10921 tagttagaaa attaagacgt cgtcctcgag atcaatttgc tatgtttata gccttagttc
    10981 tgggctggtt ttaacatcga tcgggagcac tatgctcatt ttaacaaaca atattatatt
    11041 gctgattttt ataatctata cttttttggt atttataagt gcacttggag taaaattaaa
    11101 tagcagaggg cttgataatt tttcgtgcct cattacagtc ctgacatttg ttatactcat
    11161 tttcgctcgt acaggattag gtgttgacga aagtacatat ttagaatctt atcattatta
    11221 tgttcaagga ggtgaagcag attttgaata cggatttaat atcctttttt ggggagtcag
    11281 actgttcggg gtaacagaat cttcattcaa taccatattc ccgttattga tattaacagc
    11341 attatacttc gctgttactg tatcagttaa aaatcctttt agatcttttg tattaatatt
    11401 tacaatattt tcttctttct ttctggattt ttcatttaat gcatataggc aggggctttc
    11461 attctggttt gttctacttg ccatagaatc acatctgtct gggaataggt ggaaatttat
    11521 agtttattgc ttaattggac ttggtttcca ctggtcagca gcagttgttt tattcatgct
    11581 tttcctaaca agatttttat ctacaaggtt agcgatattc tgtaatattt ttctattttt
    11641 cttaacgcta gtggcagcaa tgatgccatt acatgtactt tcaatgcttg taacgatgat
    11701 acaatacttg ccattaaact catcttactt gcaaaaagtt attttttatc ttactacaat
    11761 taagtcttcg ttttacgatt taaatttctt tggacgtgct ccattaataa tttatgcgct
    11821 tattcttatt tctctcttgg ctatatatag aaaactatta ccaattgtgc aatttaaatt
    11881 gttaattctt ttattagtct acagtgtgtt gttacttgag atgtcataca gttttagaaa
    11941 ttactactgg gtgctaccat ttactccatt tattatggcg aaaattttga gttgttactc
    12001 taataagaaa aaggtaatgg taatgtatac tatgttttct acagggttgt ggtctctatc
    12061 aatagctagt ttctattcat ttcctatact atctatgata tttcagtgaa atcatataat
    12121 aatatggttc attataaata cttttttggt taataatatt ttatataaac tgtgcattaa
    12181 cgcacggtga ccacccatga caggagtaaa caatgtcaaa gcaacagatc ggcgtcgtcg
    12241 gtatggcagt gatggggcgc aacctagcgc tcaacatcga aagccgtggt tataccgtct
    12301 ctattttcaa ccgctcccgt gagaagacgg aagaagtgat tgccgaaaat ccaggcaaga
    12361 aactggttcc ttactatacg gtgaaagagt ttgttgaatc tctggaaacg cctcgtcgca
    12421 tcctgttaat ggtgaaagca ggtgcaggca cggatgctgc tattgattct ctcaaaccat
    12481 acctcgataa aggcgacatc atcattgatg gtggtaacac cttcttccag gacaccattc
    12541 gtcgtaaccg tgagctttct gctgaaggct ttaacttcat tggtaccggt gtctctggag
    12601 gcgaagaagg tgcgctgaaa ggtccttcca ttatgcctgg tgggcagaaa gaagcctatg
    12661 aactggttgc gccgatcctg accaaaatcg ccgcagtggc tgaagacggt gagccatgcg
    12721 ttacctatat cggtgccgat ggcgcaggcc attatgtgaa gatggttcac aatggtattg
    12781 aatacggaga tatgcaactg attgctgaag cctattctct gcttaaaggt ggcctgaacc
    12841 tcaccaacga agaactggct cagaccttta ccgagtggaa taacggtgaa ctgagcagtt
    12901 acctgatcga catcaccaaa gatatcttca ccaaaaaaga tgaagacggt aactacctgg
    12961 ttgatgtgat tctggatgaa gcggctaaca aaggtaccgg taaatggacc agccagagcg
    13021 cgctggatct cggcgaaccg ctgtcgctaa ttaccgagtc tgtgtttgct cgttatatat
    13081 cttctctgaa agatcagcgc gttgccgcgt ctaaagttct ctctggtccg caagcgcagc
    13141 cagcaggcga caaagctgag tttatcgaga aagttcgtcg tgcgctgtat ctgggcaaaa
    13201 tcgtttctta tgctcagggc ttctctcagc tgcgtgcggc gtctgaagag tacaactggg
    13261 atctgaacta cggcgaaatc gcgaagattt tccgtgctgg ctgcatcatc cgtgcgcagt
    13321 tcctacagaa aatcaccgat gcttatgccg aaaatccgca gatcgctaac ctgctgctgg
    13381 ctccgtactt taaacaaatt gccgatgatt accagcaggc gctgcgtgat gtcgttgctt
    13441 atgcggtaca gaacggtatc ccggttccga ccttcgccgc tgcggttgca tattatgaca
    13501 gctaccgttc cgctgttctg cctgcgaacc tgatccaggc acagcgtgac ta
//

</pre>