LOCUS       DQ868766               10319 bp    DNA     linear   BCT 19-JUN-2007
DEFINITION  Escherichia coli serogroup O147 O-antigen gene cluster, complete
            sequence.
ACCESSION   DQ868766
VERSION     DQ868766.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10319)
  AUTHORS   Han,W., Liu,B., Cao,B., Beutin,L., Kruger,U., Liu,H., Li,Y.,
            Liu,Y., Feng,L. and Wang,L.
  TITLE     DNA microarray-based identification of serogroups and virulence
            gene patterns of Escherichia coli isolates associated with porcine
            postweaning diarrhea and edema disease
  JOURNAL   Appl. Environ. Microbiol. 73 (12), 4082-4088 (2007)
   PUBMED   17449692
REFERENCE   2  (bases 1 to 10319)
  AUTHORS   Han,W., Liu,B., Cao,B., Beutin,L., Kruger,U., Liu,H., Li,Y.,
            Liu,Y., Feng,L. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2006) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23# HongDa Street, TEDA, Tianjin
            300457, P.R. China
FEATURES             Location/Qualifiers
     source          1..10319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /note="serogroup: O147"
     misc_feature    1138..8808
                     /note="O-antigen gene cluster"
     gene            1138..2223
                     /gene="rmlB"
     CDS             1138..2223
                     /gene="rmlB"
                     /note="dTDP-D-glucose 4,6-dehydratase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlB"
                     /protein_id="ABI98981.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKTSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            2223..3125
                     /gene="rmlD"
     CDS             2223..3125
                     /gene="rmlD"
                     /note="dTDP-6-deoxy-L-mannose dehydrogenase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlD"
                     /protein_id="ABI98982.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKRIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELITTTAIK"
     gene            3180..4058
                     /gene="rmlA"
     CDS             3180..4058
                     /gene="rmlA"
                     /note="glucose-1-phosphate thymidylyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlA"
                     /protein_id="ABI98983.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKALAEPL
                     KKNAYGQYLLKMIKGY"
     gene            4063..4620
                     /gene="rmlC"
     CDS             4063..4620
                     /gene="rmlC"
                     /note="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="RmlC"
                     /protein_id="ABI98984.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFLYKTTNYYHPDSDRGIVWNDPILSIKWPTI
                     EHNNYILSIKDARAKELHNMKELFL"
     gene            4617..5849
                     /gene="wzx"
     CDS             4617..5849
                     /gene="wzx"
                     /note="O-antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ABI98985.1"
                     /translation="MSIVKNTLWNISGYIIPSLIAIPALGILSRILGAEQFGLFTLAI
                     ALVGYASIFDAGLTRAVIREVSIYKNVHKELRSIISTSTVILTILGLIGGSVLFLSSN
                     VIVKLLNINANHVVESVKAIYIISATIPLYLLNQVWLGIFEGMEKFRKVNLIKSINNS
                     FVAGLPVIFCFFHGGLLSAIYGLVMARVLSLIVTFIFSRKLIISSGLSVKIVTVKRLI
                     GFGSWITVSNIISPIMTYMDRFILSHIVGADKVSFYTAPSEGIQRLTILPSALSRAIF
                     PRLSSELQSVKQTKILSYFIMVIGILPIVILIIILSDFIMSAWMGPTYHGTPGIVLKI
                     LAIGFFFNCIAQIPFVSVQASGRSKITAIIHLLEVIPYLCILYIFIYHWGIVGAAIAW
                     SVRTSLDFLILLLIDKKY"
     gene            5905..7092
                     /gene="wzy"
     CDS             5905..7092
                     /gene="wzy"
                     /note="O-antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ABI98986.1"
                     /translation="MTSDFYNSKDKSLSVLLFFGFIFFLTRSFPFIQYSWIMGGFLCL
                     CIMSFTKKIANGIYHYPVILIFLLALFINFIYSYIKGNDIAIIIRFYIIILFILCAYF
                     CSYGTISIVKIFLYLMVLQAVIISIISIYMTKTYGIGDYSALRHYFLENDYGDVYTYG
                     SGFYRVQIKGNALIPFAFMLHIVIKDYFYYRFKNTITVILAIGTIVAGNFAYFVSICL
                     FFMYIILCSKSNSRYAKLRKIIFGVFLTVILPFFITYSIELIIMKSNGADSSLGVRWD
                     QFTVLINDLTESVSNFVIGSGLGNVIKIQTPIRDYSAYIYYELQSVYFLNQLGVILFT
                     LFLLINLLLTIKIIKYSELCVLYFLYVSYAITNPYILDSNHVAVIIVLVTLSNVLKKM
                     KAK"
     gene            7109..7978
                     /gene="wfbY"
     CDS             7109..7978
                     /gene="wfbY"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfbY"
                     /protein_id="ABI98987.1"
                     /translation="MDTVYAVLVAYNPEHNDLKNAVELLLRQVTKVVVCNNSTNGYKY
                     AENSSGDVKIFNFNDNLGIAEAQSIGMKWAFENGADFILQMDQDSIPDPKMVEQLLTC
                     YKKLLKQNVNVGLVGSQDFDKVTGELNKARVKKGKPLTEVYYEVDSTLSSGSLIPKNS
                     WLIVGGMKDELFIDAVDHEYCWRLRAAGFKVIRNKNALLAHRLGDGRFKILNILSVGL
                     PSPFRHYYATRNIFLLLNKKYVPIYWKISSLVKLIGKVFLYPIFLPNGNKRLYFFLKG
                     INDGLMGRSGKMK"
     gene            7975..8808
                     /gene="wfbZ"
     CDS             7975..8808
                     /gene="wfbZ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfbZ"
                     /protein_id="ABI98988.1"
                     /translation="MNHRLEKFSVLISIYKNDLPQFFEVALRSIFHDQTLKPDQIVIV
                     ADGELHQTHIDIINSFIDDVGNKIVTFVPLPRNVGLANALNEGLKACRNELVARMDAD
                     DISLPHRFEKQISFMINNSEIDVCGSFIDEIETVTEEFISTRKVPLEHREIVKFARKR
                     SAVSHPSVIFRKNTVLAVGGYPPFRKSQDFALWSLLIVHNARFANLPDILLKMRTGRN
                     LMARRGLSYLLYEYKVLYYQYKIGFIRKNELISNAMLRTFFRIMPSKLKELMYSIVRN
                     R"
ORIGIN      
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc atgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctctcc ttgaacagcg cgtgaagcgt
      121 caactactgg cggaagtaca atctatctgt ccgccgggcg tgaccattat gaacgtgcgt
      181 cagggtgaac ctttaggttt aggccactcc attttatgtg cgcgacctgc cattggtgac
      241 aatccatttg tcgtggtgct gccagacgtt gtgatcgacg acgccagcgc cgacccgcta
      301 cgctacaacc ttgctgccat gattgcgcgc ttcaatgaaa cgggccgtag ccaggtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactccgtca tccagaccaa agaaccgctg
      421 gaccgtgaag gcaaagtcag ccgcattgtt gaatttatcg aaaaaccgga tcagccgcaa
      481 acgctggact cagacatcat ggccgtaggt cgttatgtgc tttctgccga tatttggccg
      541 gaacttgaac gcactcaacc tggtgcatgg gggcgtattc agctgactga tgctattgcc
      601 gaactggcga aaaaacagtc cgttgatgcc atgctgatga ctggtgacag ctacgactgt
      661 ggtaaaaaaa tgggctatat gcaggcattc gtgaagtatg gattacgcaa cctgaaagaa
      721 ggggcgaagt tccgtaaagg gattgagaag ctgttaagcg aataatgaaa atctgaccgg
      781 atgtaacggt taataagaaa attataacgg cagtgaggat tcgtggcgaa agtaatttgt
      841 tgcgaatatt cctgccgttg ttttatataa acaatcagaa taacaacgag ttagcaatag
      901 gattttagtc aaagttttcc aggattttcc ttgtttccag agcggattgg taagacaatt
      961 agcttttgaa tttttcgggt ttagcgcgag tgggtaacgc tcgtcacatc gtaggcatgc
     1021 atgcagtgct ctggtagctg taaagccagg ggcggtagcg tgcattaata cttctattaa
     1081 tcaaactgag agccgcttat ttcacagcat gctctgaagc aatatggaat aaattaggtg
     1141 aaaatacttg ttactggtgg cgcaggattt attggttctg ctgtagttcg tcacattata
     1201 aataatacgc aggatagtgt tgttaatgtc gataaattaa cgtacgccgg aaacctggaa
     1261 tcacttgctg atgtttctga ttctgaacgc tatgtttttg aacatgcgga tatttgcgat
     1321 gcagctgcaa tggcacggat ttttgctcag catcagccag atgcagtgat gcacctggct
     1381 gctgaaagcc atgttgaccg ttcaattaca ggtcctgcgg catttattga aaccaatatt
     1441 gttggtacat atgtcctttt ggaagccgct cgcaattatt ggtctgctct tgatagcgac
     1501 aagaaaacta gcttccgttt tcatcatatt tctactgacg aagtctatgg tgatttgcct
     1561 catcctgacg aggtaaataa tacagaagaa ttacccttat ttacagagac aacagcttac
     1621 gcgccaagca gcccttattc cgcttcaaaa gcatccagcg atcatttagt ccgcgcgtgg
     1681 aaacgtacct atggtttacc aaccattgtg actaattgct ctaacaatta tggtccttat
     1741 catttcccgg aaaaattgat tccattggtt attctcaatg ctctggaagg taaggcatta
     1801 cctatttatg gcaaagggga tcaaattcgt gactggctgt atgttgaaga tcatgcgcgt
     1861 gcgttatata ccgtcgtaac cgaaggtaaa gcgggtgaaa cttataacat tggtggacac
     1921 aacgaaaaga aaaacatcga tgtagtgctc actatttgtg atttgctgga tgagattgta
     1981 ccgaaagaga aatcttaccg cgagcaaatt acttatgttg ccgatcgccc gggacacgat
     2041 cgccgttatg cgattgatgc agagaagatt agccgcgaat tgggctggaa accgcaggaa
     2101 acgtttgaga gcgggattcg gaagacagtg gaatggtacc tgtccaatac aaaatgggtt
     2161 gataatgtga aaagtggtgc ctatcaatcg tggattgaac agaactatga gggccgccag
     2221 taatgaatat cctccttttc ggcaaaacag ggcaggtagg ttgggaacta cagcgtgctt
     2281 tggcaccttt aggtaatttg attgctcttg atgttcactc cactgattat tgtggtgatt
     2341 ttagtaatcc tgaaggtgta gctgaaacag tcaaaagaat tcgacctgat gttattgtta
     2401 atgctgcggc tcacaccgca gtagataagg ctgagtcaga acccgaattt gcacaattac
     2461 tcaatgcgac tagtgttgaa tcaattgcaa aagcggctaa tgaagttggg gcttgggtaa
     2521 ttcattactc aactgactac gtattccctg gaaatggcga cacgccatgg ctggagacgg
     2581 atgcaaccgc accgctaaat gtttacggtg aaaccaagtt agccggagaa aaagcgttac
     2641 aggaacattg cgcgaagcat cttattttcc gtaccagctg ggtatacgca ggtaaaggaa
     2701 ataacttcgc caaaacgatg ttgcgtctgg caaaagagcg cgaagaactg gctgtgataa
     2761 atgatcaatt tggtgcgcca acaggtgctg agctgctggc tgattgtacg gcacatgcta
     2821 ttcgtgtggc actgaataaa ccggaagtcg caggtttgta ccatctggta gccagtggta
     2881 ccacaacctg gcacgattat gctgcgctgg tttttgaaga ggcgcgcaaa gcaggtattc
     2941 cccttgcact caacaagctc aacgcagtac caacaacagc ctatcctaca ccagctcgtc
     3001 gtccacataa ctctcgcctt aatacagaaa aatttcagca gaactttgcg cttgtcttgc
     3061 ctgactggca ggttggtgtg aaacgaatgc tcaacgaatt aattacgact acagcaatta
     3121 aatagttttt gcatcttgtt cgtgatggtg gagcaagatg aattaaaagg aatgatgaaa
     3181 tgaaaacgcg taaaggtatt attttagcgg gtggttctgg tacacgtctt tatcctgtga
     3241 ctatggctgt cagtaaacag ctattaccta tttatgataa gccgatgatc tattacccgc
     3301 tctctacact gatgttggcg ggtattcgcg atattctgat tattagtacg ccacaggata
     3361 ctcctcgttt tcaacaactg ctgggtgacg gtagccagtg ggggctaaat cttcagtaca
     3421 aagtgcaacc gactccagat gggcttgcgc aggcgtttat tatcggtgaa gagtttatcg
     3481 gtggtgatga ttgtgctttg gttcttggtg ataatatctt ctacggtcat gatctgccga
     3541 agttaatgga tgtcgctgtt aacaaagaaa gtggtgcaac ggtatttgcc tatcacgtta
     3601 atgatcctga acgctacggt gtcgttgagt ttgataaaaa cggtacggcg atcagcctgg
     3661 aagaaaaacc gctacaacca aaaagtaatt atgcggtaac cgggctttat ttctatgata
     3721 acgacgttgt cgaaatggcg aaaaacctta agccttctgc ccgtggtgaa ctggaaatta
     3781 ccgatattaa ccgtatttat atggaacagg ggcgtttatc cgttgccatg atggggcgtg
     3841 gttatgcatg gctggatacg gggacacatc agagtcttat tgaagcaagc aacttcattg
     3901 ccaccattga agagcgccag ggactaaagg tttcctgccc agaagaaatt gcttaccgta
     3961 aagggtttat tgatgctgaa caggtgaaag cattagcgga gccgctgaaa aaaaatgctt
     4021 atggacagta tctgctgaaa atgattaaag gttattaata aaatgaacgt aattaaaaca
     4081 gaaattcctg atgtgttaat tttcgagccg aaagtttttg gtgatgagcg tggtttcttt
     4141 atggaaagct ttaatcagaa agttttcgaa gaagctgtag gacgtaaggt tgaatttgtt
     4201 caggataacc attcgaagtc tagtaaaggt gttttacgcg ggctgcatta tcagttagaa
     4261 ccttatgcgc aagggaaatt ggtacgttgc gttgttggtg aggtttttga tgtagctgtt
     4321 gatattcgta aatcgtcgcc tacctttggt aaatgggttg gggtgaattt atctgctgag
     4381 aataagcggc aattgtggat ccctgaggga tttgcacatg gttttttggt gctgagcgag
     4441 actgcggaat ttttatataa aacgacgaac tattatcatc ctgatagtga tagagggatt
     4501 gtatggaatg atcctattct gagcataaaa tggccgacga tagaacataa taattatatt
     4561 ttatcgatta aagatgcaag ggctaaagaa ttgcataaca tgaaggaatt atttttgtga
     4621 gtattgtaaa gaatacttta tggaatataa gtgggtatat tataccatca ttaatagcaa
     4681 ttcctgcgtt aggtatactg tctagaattc taggggccga gcaatttggc ctttttacgt
     4741 tagctattgc cttagttgga tatgcaagta tttttgatgc tggattgacc agagctgtta
     4801 taagagaagt atcaatatat aaaaatgttc ataaagaatt aagatcgatc atttcaactt
     4861 caacggtaat tctaactata ttgggcttga ttggcggtag tgtattattt ttgagtagca
     4921 atgtaattgt taaattatta aacattaacg cgaatcatgt tgtagaatct gtcaaagcaa
     4981 tatatattat ttcagctacc atacccttat acttgttaaa ccaagtctgg ttggggattt
     5041 ttgaggggat ggaaaagttc agaaaagtaa atttaataaa atcaattaac aattcttttg
     5101 tggctggatt accagtgatt ttctgttttt ttcatggagg attactaagt gccatatatg
     5161 gtttagttat ggcaagagtc ttatcactta tagtgacctt tatatttagt cgaaaactaa
     5221 taatatcatc tgggctgtct gtaaaaattg taacagttaa aagattaatc ggctttggaa
     5281 gctggataac agttagcaat attattagcc ctattatgac atatatggat cgttttattc
     5341 tttcacacat tgtgggggct gataaagttt ctttttatac tgctccgtct gaaggtatac
     5401 aacgcttaac gatattacca agtgcgttgt ccagagctat ttttccaaga ttaagttcag
     5461 aattgcaatc ggtaaagcaa actaaaatat tatcatattt tataatggtt attggtatac
     5521 ttccaattgt aattttgata attattttat cagattttat aatgtccgct tggatgggac
     5581 ctacatatca tgggacgcca ggtatagtat taaaaattct tgcaataggt ttctttttta
     5641 attgcattgc acaaatccca tttgtttcag ttcaggctag tggaagatca aaaattacag
     5701 ctattattca tttgctcgaa gttatcccat atttatgcat attatatatt tttatttatc
     5761 attggggaat tgttggagcc gcaatagcat ggtctgtaag aacatcgtta gattttttga
     5821 tattattatt aattgataag aaatattaat agcgaattga ttttagggat tacttcctca
     5881 agcccatcta attagagtgc aaacatgact tctgattttt ataactcaaa agacaaaagt
     5941 ttaagtgttc ttttgttttt tgggtttata tttttcctta cacgtagctt tccatttatt
     6001 caatatagtt ggattatggg ggggttttta tgtctttgta tcatgtcatt tacaaagaaa
     6061 attgcaaacg gaatatatca ctatcctgtt attttaatat ttctattagc tctttttata
     6121 aattttattt attcctatat caagggtaac gatatagcga taataattag gttttatatt
     6181 atcatattat ttatattatg tgcttatttt tgctcttatg gaaccatctc gattgttaaa
     6241 atatttttat atttaatggt attacaggcg gttattatat ccatcattag tatttatatg
     6301 acaaaaacat atggtattgg tgattattca gcactaagac attatttttt ggagaatgat
     6361 tatggtgatg tttatacata tggaagtggt ttctatagag ttcaaattaa aggaaatgct
     6421 ctcattccat ttgcctttat gttgcatata gtcataaaag attatttcta ttatcgattc
     6481 aaaaatacaa taaccgttat tctggctata ggtactatag tggctggtaa ttttgcatat
     6541 tttgtttcga tatgcttgtt ttttatgtat attatactat gttctaaatc taactcacga
     6601 tacgctaaat taaggaaaat tatttttggg gtttttctta ctgtgattct cccttttttt
     6661 attacatatt caattgagtt gataatcatg aaatcaaatg gagctgattc ttctttagga
     6721 gttagatggg atcagtttac tgtattaatt aatgatctta cagagtctgt atctaatttt
     6781 gttataggtt ctggtttggg taatgtcatc aaaattcaaa ctcctatccg tgattatagt
     6841 gcatatatat attatgaatt gcagtcagtt tattttttaa atcaacttgg cgttatttta
     6901 tttactttgt ttttattaat taatctgctt ctcacgatta aaatcataaa atacagtgag
     6961 ttgtgtgtgc tatattttct atatgtttct tatgcaatta ctaatcctta tattttagac
     7021 tctaaccatg ttgctgtaat aattgtatta gtgacattaa gtaatgttct aaaaaagatg
     7081 aaagctaaat gaaggtttta aggtgaagat ggacactgta tatgccgttt tggttgctta
     7141 caacccagaa cataatgatt taaaaaatgc ggttgaatta ttgttgagac aagttactaa
     7201 agttgtcgtt tgcaataact ctacaaatgg ttataaatat gctgaaaatt cttcaggcga
     7261 tgtaaaaata ttcaatttca atgataattt aggcatagca gaagcccaaa gtataggaat
     7321 gaaatgggct tttgaaaatg gtgctgattt tatattgcaa atggatcagg atagtattcc
     7381 tgatcctaag atggtagagc agttacttac ttgttacaaa aaattgctta aacaaaatgt
     7441 caatgttggt ttagtcggtt cacaagattt tgataaagta actggtgaat taaataaagc
     7501 aagggtaaaa aaagggaaac cacttacaga agtttattat gaggtagata gtacattaag
     7561 ttctggcagt ctaataccaa aaaatagttg gttgattgtt ggaggaatga aagatgagct
     7621 ttttatcgat gcggtagacc atgaatattg ttggagatta agagctgctg ggtttaaagt
     7681 aattaggaat aaaaatgcgt tacttgcaca tagacttgga gatgggcgat ttaagatctt
     7741 aaatattctt tctgtcggtt tgccaagccc atttcgtcat tattatgcta ctcgaaatat
     7801 ctttctttta ttaaataaaa aatatgtacc catctactgg aaaatttcta gtctggttaa
     7861 attaattgga aaggtttttt tatatcctat tttccttcca aatggtaata aaaggttata
     7921 ttttttttta aaaggcatta atgacggttt aatgggtcga agtggtaaaa tgaaatgaat
     7981 catagattag aaaaattctc agttttaatt agcatttata aaaatgatct accgcaattt
     8041 tttgaggtgg ctctacgctc tatttttcac gatcaaacac ttaagccaga tcaaatagta
     8101 attgttgcag atggagaact ccatcaaaca cacatcgata ttataaattc attcattgat
     8161 gatgttggca ataaaatagt aacatttgta cctttaccta gaaatgttgg attggctaat
     8221 gccttaaatg aaggattaaa ggcttgtagg aatgagttag tggcaagaat ggatgctgat
     8281 gatatttctt tgcctcatcg gtttgagaaa caaatttctt ttatgattaa taattcagaa
     8341 atagatgtat gtggcagttt tattgatgaa attgaaactg ttactgagga gtttatttca
     8401 acacgcaaag tgcctctcga acatagagaa atagttaaat tcgcgaggaa acgaagcgca
     8461 gttagccatc cttctgtaat ttttagaaag aatacagtat tagctgttgg tggttatcct
     8521 ccattcagaa aatctcaaga ttttgcatta tggagcctat taattgtaca taatgcaaga
     8581 tttgcaaatc ttccagatat tttattaaaa atgcgaactg gtcgtaatct tatggctcga
     8641 cgtggattgt catatttatt gtacgagtat aaagtattgt attatcaata taaaattggt
     8701 tttattcgaa aaaatgaatt aataagtaat gctatgttga gaacattttt tcgtataatg
     8761 ccatctaaat taaaggagct gatgtattca atcgttagga atcgataata ataattttct
     8821 gattaagtgt tatggatttg tttttattag gcatattcta taattaagca taacccgcat
     8881 accacccagc ggtatcctga caggagtaaa caatgtcaaa gcaacagatc ggcgtcgtcg
     8941 gtatggcagt gatggggcgc aaccttgcgc tcaatatcga aagccgtggt tataccgtct
     9001 ctattttcaa ccgttcccgt gaaaagacgg aagaagtgat tgccgaaaat ccaggcaaga
     9061 aactggttcc ttactatacg gtgaaagagt ttgttgaatc tctggaaacg cctcgtcgca
     9121 tcctgttaat ggtgaaagca ggtgcaggca cggatgctgc tattgattcc ctcaaaccat
     9181 atctcgataa aggcgacatc atcattgatg gtggtaacac cttcttccag gacaccattc
     9241 gtcgtaaccg tgagctttct gctgaaggct ttaacttcat tggtaccggt gtctccggtg
     9301 gagaagaagg cgcgctgaaa ggtccttcca ttatgcctgg tgggcagaaa gaagcctatg
     9361 aactggttgc gccgatcctg accaaaatcg ccgcagtggc tgaagacggt gagccatgcg
     9421 ttacctatat tggtgccgat ggcgcaggtc actatgtgaa gatggttcac aacggtatcg
     9481 aatacggtga tatgcaactg attgctgaag cctattctct gcttaaaggt ggcctgaacc
     9541 tcaccaacga agaactggcg cagaccttta ccgaatggaa taacggtgaa ctgagcagct
     9601 acctgatcga catcaccaaa gatatcttca ccaaaaaaga tgaagacggt aactacctgg
     9661 ttgatgtgat tctggatgaa gcagcaaaca aaggtacggg taaatggacc agccagagtg
     9721 cgctggatct cggtgaacca ctgtcgctta ttaccgagtc tgtgtttgca cgttatatct
     9781 cttctctgaa agatcagcgc gttgccgcat ctaaagttct ctctggtccg caagcgcagc
     9841 cagctggcga caaggctgag ttcatcgaaa aagttcgtcg tgcactgtat ctgggcaaaa
     9901 tcgtttctta cgcccaaggc ttctctcagc tgcgtgctgc gtctgaagag tacaactggg
     9961 atctgaacta cggcgaaatc gcgaagattt tccgtgctgg ctgcatcatc cgtgcgcagt
    10021 tcctgcagaa aatcaccgat gcatacgccg aaaatccgca aatcgctaat ctgctgctgg
    10081 ctccgtactt caagcaaatt gccgatgact accagcaggc gctgcgtgat gtcgttgctt
    10141 atgcagtaca gaacggtatc cctgttccga ccttcgctgc tgcggttgcc tattacgaca
    10201 gctaccgcgc cgctgttctg cctgcgaacc tgatccaggc acagcgtgac tatttcggtg
    10261 cgcatactta taagcgcatt gataaagaag gtgtgttcca taccgaatgg ctggattaa
//