<pre>LOCUS       DQ465249               11888 bp    DNA     linear   BCT 13-AUG-2007
DEFINITION  Escherichia coli strain O146 O antigen gene cluster, complete
            sequence.
ACCESSION   DQ465249
VERSION     DQ465249.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11888)
  AUTHORS   Liu,Y., DebRoy,C. and Fratamico,P.
  TITLE     Sequencing and analysis of the Escherichia coli serogroup O117,
            O126, and O146 O-antigen gene clusters and development of PCR
            assays targeting serogroup O117-, O126-, and O146-specific DNA
            sequences
  JOURNAL   Mol. Cell. Probes 21 (4), 295-302 (2007)
   PUBMED   17452091
REFERENCE   2  (bases 1 to 11888)
  AUTHORS   Liu,Y., Debroy,C. and Fratamico,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2006) ERRC-USDA, 600 E. Mermaid Lane, Wyndmoor,
            PA 19038, USA
FEATURES             Location/Qualifiers
     source          1..11888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="O146"
                     /db_xref="taxon:562"
     misc_feature    1..11888
                     /note="O antigen gene cluster, complete sequence"
     gene            301..1194
                     /gene="rmlB"
     CDS             301..1194
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="ABE98429.1"
                     /translation="MARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIVGTYVLL
                     EAARNYWSALDSDKKNSFRFHHISTDEVYGDLPYPDEVNNKEELPLFTETTAYAPSSP
                     YSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALPIY
                     GKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVP
                     KEKSYREQITYVTDRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWYLSNTKW
                     VDNVKSGAYQSWIEQNYEGRQ"
     gene            1194..2093
                     /gene="rmlD"
     CDS             1194..2093
                     /gene="rmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dyhydrorhamnose reductase"
                     /protein_id="ABE98430.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWQEADATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRMALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIALALNKLNAVPTTAYPTPARRPHNSRLDTEKFQQNFA
                     LVLPDWKIGVKRMLNELFTTTAI"
     gene            2151..3029
                     /gene="rmlA"
     CDS             2151..3029
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="ABE98431.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGNQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            3034..3567
                     /gene="rmlC"
     CDS             3034..3567
                     /gene="rmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-D-glucose-3,5 epimerase"
                     /protein_id="ABE98432.1"
                     /translation="MNVIKTVIPEVLIFEPKKFEDSRGYFFESFNQRLFEDAVGKETL
                     FVQDNQSYSSKNVLRGLHYQSEPYAQGKLVRCIMGEVFDVAVDIRKESESYGQWVGVF
                     LSEDNNRQLWIPEGFAHGFLVKSDKAIFAYKCTNFYNPSAENTIRWDSPDLAIEWPLD
                     DEDLLISEKDLLGRKFL"
     gene            3582..4574
                     /gene="wbuD"
     CDS             3582..4574
                     /gene="wbuD"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="ABE98433.1"
                     /translation="MMYKKIKMLGRKLRCEYNILRGFIYDYKIYNNASLKTHKKTMGF
                     DESLGLIIRLYHSLEKGLANEKFKKESSLKNVSLLLTIFLENNKIDYSDVHVQAAIKT
                     VSLYFEKHPNKGTLEFEIQRRKFKQILEKVGDVSGYIGGVKQIPTKPKNQEIDYKEFV
                     KTRVSVRSFSGKKIILEDILKVIDIARYCPSACNRQAVKLFYSLNSKENEHILKLQNG
                     SRSFREAVPGLIVITSDLRYQEGSEERNLGFIEGGIWISSLVNSLHAYNIGSCVLNWC
                     VNPETDKKLRDLINIPYNYQIISLLAIGYANNDQLVPFSVRKEAIDFIQNINLK"
     gene            4571..6109
                     /gene="wzx"
     CDS             4571..6109
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="ABE98434.1"
                     /translation="MSIKMKANTSNTKRLFFNSIIMYLRMILLMLITLYISRVTINTL
                     GIEKFGLYSVISGFVLLAGVFTNILESTSQRFLSKSLGEGGNVSSIFESCISTHIVLS
                     LIAALLIEMLGVWFINYKLRQDVISINEVYIIFHLSVITFVVTVITSPFYAALISFEN
                     VNYYSIFTLCDVISKVVGISLAPQLPWEITINYAAMILITTLVNRVILTSCFVMRYPK
                     LGCRPSLDFCQMKNILSFAVWNMWGGAATVLNSQGINIVINVFFGILFNSARAITSQI
                     NSAILQLTNSIQLALNPQIVKSYFKDNSCYLNQLIICSGKINVYLTLLIICIFLPNIN
                     FLLTVWLGNFPVMTILLIKLMFIELFINSFSASLSSLIQATGKIKIYQMVVGGVMLLN
                     LPLCLLILSIFKEVEIVYLVAIFVALVCLVLRLIFVNMNSKINIVSYLTNVILPGGGI
                     AFIIYLIQNRWLTEATDLLTALINILYTLFLYSVLIIVIGLNNTERCFVLKHCGFFNW
                     MKNG"
     gene            6102..7205
                     /gene="wbuE"
     CDS             6102..7205
                     /gene="wbuE"
                     /codon_start=1
                     /transl_table=11
                     /product="unknown"
                     /protein_id="ABE98435.1"
                     /translation="MAKLKTKSSNCGIITFHKAHNYGAVLQAYALMTTLKKNGLQVSF
                     IDYENEVLSRGYSFYPILKGTSTINYIKEWIHLILDLKRKYKRFKAFSDFINKYIACT
                     PLNKDTKCFDIIFLGSDQIWNANYTNGVDPNYYGQGPYCKAHKLVSYAASMGKLCLGK
                     YEEQAFLSLINNIQQIGVRENYLKTYIEEKTDLKCDVNLDPTLLLTKSDWDKLAAPND
                     TQERYLLIYEMHTHRSTNIIANKIAKILNLKIKKLACRTNYKIEKDVITNAGPQNFLT
                     LFKNAAFVVTTSFHGTVFSIINQVPFFTLEFGNEIDLRSRSLLEMLNLNERMISDDAN
                     LNYEKLFLEFDEAHSILESKRQDSLSFIERALS"
     gene            7552..8352
                     /gene="wbwU"
     CDS             7552..8352
                     /gene="wbwU"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ABE98436.1"
                     /translation="MGLLLSIFLKKKNIFHIREFGLEDQHQKHIITDHLYYSLVNKYA
                     NEVIVISEALKNKIIKYITGNNLNLIYDDVHIQNKPMLNYANSARLRKFIIIGTLCEG
                     KGQKIAIEAMHNLIREGYLCHLKIIGNNRVPYASYLNKIVADYNLSDYVEFMGFRHDL
                     DQIRLDNDVCLIPSLSEAFGRVTIESMAAGMIVVASDSGASKEIINDGINGFLFSSGS
                     VSDLTSVLKKILDVESNNLECIRKRALVDSQKYTSGHAASSIYNLIIN"
     gene            8472..9650
                     /gene="wzy"
     CDS             8472..9650
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="ABE98437.1"
                     /translation="MIFFLFRQYPLLKYSSIMNVSCLLFLIFVTFFIKRGVFSSGIFL
                     FSLIFLSVHGLYSLYSGNDPVLISRFYIILILIIVSYYAAIKSISYINIFIFLAVTQA
                     FVIIGLEAFMFLNFRFSDYSAIRNYFIDNQFGDIYTYNGFFYHIQIKGNALLLFSYMI
                     SFYLYNKTNNKLYLLSSMFLALAVLFCGNLAFYITFLIHAFIFLFLRKARTYNQLLLK
                     VFIFLITFGVFISYSGMEYLVKAYELKFQGANFSSMGTRFDQFNVLIDDLFENVLTAL
                     VGQGLGNIINVQTAVRNYSDYIYYELQAVYFLNQLGVLLFLLFVIINVILTLKFIKPI
                     ELRIVYMLYILYALVNPYMLDTNHVVVVFILVSLSSIFSKGGDCSYNGKKHISSYNYI
                     "
     gene            9613..10500
                     /gene="wbwV"
     CDS             9613..10500
                     /gene="wbwV"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ABE98438.1"
                     /translation="MEKNISVVIITFNPKVEILKENIINLLKSSIVIKVIIVDNGASK
                     SLDELKSIPDVIIKHLGQNRGIATAQNVGLRISQSLCSKYALLLDQDSEIETDLVSKL
                     LDGYITLEQKGIKLAAVGPRPFDLFENKKMKASIQRDIQIFEGFSLTKQIIASGKLIN
                     LDVLDQVGFMEDDLFIDGVDHEWCWRAGRLGYKIAIIENAIMKHRLGDARGNFLGFTY
                     KIGSPIRLYYQFRNILVLSRRDYVPLYWKLRNLIGMFLKFIIFSLKENDSKIRRYYMI
                     KGFCSGLKKDKGIIDKPFK"
     gene            10550..11362
                     /gene="wbwW"
     CDS             10550..11362
                     /gene="wbwW"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="ABE98439.1"
                     /translation="MFKFSVLMSVYYKEKPAYLDEALNSLAIQTYKADEIVLVIDGQI
                     PIQLKNVIDKWLLLLPIKTIPLEKNVGLGNALNIGLNYVTNKLVARMDTDDICVPERF
                     EKQISYFQKNPSTIILGGAIDEYDETFKDRRGRRFSCCSHSDIVSYSLFRNPFNHMTV
                     MFNKEFILGIGGYKHHHYMEDYNLWLRCIANGAICSNIDNTLVLARTGDAMIERRRGR
                     DYLKSEIELARLKIKCFPNKKAKIILITFSRLIVRILPQQILSSVYKNIRKK"
ORIGIN      
        1 tattggtagc tgtaagccaa gggcggtagc gtgcattaat acctctatta atcaaactga
       61 gagccgctta tttcacagca tgctctgaag taatatggaa taaattaagt gaaaatactt
      121 gttactggtg gcgcaggatt tattggttct gctgtagttc gtcacattat aaataatacg
      181 caggatagtg ttgttaatgt cgataaatta acgtacgccg gaaacctgga atcacttgct
      241 gatgtttctg attctgaacg ctatattttt gaacatgcgg atatttgcga tgctgctgca
      301 atggcacgga tttttgctca gcatcagccg gatgcagtga tgcacctggc tgctgaaagc
      361 catgttgacc gttcaattac aggtcctgcg gcatttattg aaaccaatat tgttggtact
      421 tatgtccttt tagaagccgc tcgcaattac tggtctgctc ttgatagcga caagaaaaat
      481 agtttccgtt ttcatcatat ttctactgac gaagtctatg gtgatttacc ttatcctgac
      541 gaagtaaata ataaagaaga attaccctta tttactgaga caacagctta cgcaccaagc
      601 agcccttatt ccgcatcaaa agcatccagc gatcatttag tccgcgcgtg gaaacgtacc
      661 tatggtttac cgaccattgt gactaactgc tctaacaatt atggtcctta tcatttcccg
      721 gaaaaattga ttccattggt tattctgaat gctctggaag gtaaagcatt acctatttat
      781 ggtaaagggg atcaaattcg tgactggctg tatgttgaag atcatgcgcg tgcgttatat
      841 accgtcgtaa ccgaaggtaa agcgggtgaa acttataaca ttggtggaca caacgaaaag
      901 aaaaatatcg atgtagtgct cactatttgt gatttgttgg atgagattgt cccgaaagag
      961 aaatcttacc gcgagcaaat tacttatgtt accgatcgtc cgggacacga tcgccgttat
     1021 gcgattgatg ctgagaagat tggtcgcgaa ttgggatgga aaccacagga aacgtttgag
     1081 agcgggattc gtaaaacagt ggaatggtac ctgtccaata caaaatgggt tgataatgtg
     1141 aaaagtggtg cctatcaatc gtggattgaa cagaactatg agggccgcca gtaatgaata
     1201 tcctcctttt cggcaaaaca gggcaggtag gttgggaact acagcgtgct ctggcacctc
     1261 tgggtaattt gattgctctt gatgttcact ccactgatta ctgtggtgat tttagtaatc
     1321 ctgaaggtgt agctgaaacc gtaagaagca ttcggcctga tattattgtc aacgcagcag
     1381 ctcacaccgc agtagacaaa gcagaatcag aaccggagtt tgcacaatta cttaacgcga
     1441 cgagtgtcga agcgatcgcg aaagcagcca atgaagtcgg cgcatgggtt attcactact
     1501 ctactgacta cgtatttccg gggaccggtg aaataccatg gcaggaggcg gatgcaaccg
     1561 caccgctaaa tgtttacggt gaaaccaagt tagctggaga aaaagcatta caggaacatt
     1621 gcgcgaagca tcttattttc cgtaccagct gggtatacgc aggtaaagga aataacttcg
     1681 ccaaaacgat gttgcgtctg gcaaaagagc gcgaagaact ggctgtgatt aatgatcaat
     1741 ttggtgcgcc aacaggtgct gagctgctgg cagattgtac ggcacatgca attcgtatgg
     1801 cactgaataa accagaagtc gcaggcttgt accatctggt agccagtggt accacaacct
     1861 ggcacgatta tgctgcgctg gtttttgaag aggcgcgcaa agcaggcatt gcccttgcac
     1921 tcaacaagct caacgcagta ccaacaacag cctatcctac accagctcgt cgtccgcata
     1981 actctcgcct tgatacagaa aaatttcagc agaactttgc gcttgtcttg cctgactgga
     2041 agattggcgt gaaacgcatg ctcaacgaat tatttacgac tacagctatt taatagtttt
     2101 tgcatcttgt tcgtgatggt ggagcaagat gaattaaaag gaatgatgaa atgaaaacgc
     2161 gtaaaggtat tattttagcg ggcggttctg gtactcgtct ttatcctgta actatggctg
     2221 tcagtaaaca gctattaccg atttatgata aaccgatgat ctattatccg ctctctacac
     2281 tgatgttagc gggtattcgc gatattctga ttatcagtac gccacaggat actcctcgtt
     2341 ttcaacaact gctgggtgac gggaaccagt ggggcctgaa tcttcagtac aaagtgcaac
     2401 cgagtccgga tggtcttgcg caggcattta tcatcggtga agagtttatc ggtggtgatg
     2461 attgtgcttt ggtacttggt gataatatct tctacggtca cgacctgcct aagttaatgg
     2521 atgccgctgt taacaaagaa agtggtgcaa cggtatttgc ctatcacgtt aatgatcctg
     2581 aacgctacgg tgtcgttgag tttgataaaa acggtacggc gatcagcttg gaagaaaaac
     2641 cgctacaacc aaaaagtaat tatgcggtaa ccgggcttta tttctatgat aacgacgttg
     2701 tggaaatggc gaaaaacctt aagccttctg cccgtggtga actggaaatt accgatatta
     2761 accgtattta tatggaacag gggcgtttat ccgttgccat gatgggacgt ggttatgcat
     2821 ggctggacac ggggacacat caaagtctta ttgaggcaag caacttcatt gcaacaattg
     2881 aagagcgtca ggggctaaaa gtttcctgcc cagaagaaat tgcttaccgt aaagggttta
     2941 ttgatgctga gcaggtgaaa gtattagctg aaccgctgaa aaaaaatgct tatggtcagt
     3001 atctgctgaa aatgattaaa ggttattaat aaaatgaacg taattaaaac agtaattcca
     3061 gaagtcttga tttttgagcc taaaaaattt gaggactcca gggggtattt ttttgaaagc
     3121 tttaatcaaa ggctttttga agatgccgta ggaaaagaaa ctctattcgt ccaagataat
     3181 caatcatatt caagtaaaaa tgtattaaga ggtttacatt atcaaagcga gccgtatgct
     3241 caagggaagc tagtaagatg tattatgggg gaagtatttg atgtagccgt agatattaga
     3301 aaagagtcag aaagttatgg gcaatgggtt ggagtatttt tatctgagga taataataga
     3361 cagttatgga tacctgaagg atttgctcac ggttttcttg ttaaaagcga taaagcaatt
     3421 tttgcatata aatgtactaa tttttataac ccttctgctg aaaatactat tagatgggac
     3481 tcgcctgatt tggcaattga atggccttta gatgatgaag atttattgat atcggaaaaa
     3541 gatttattag gaagaaaatt tttataacga caggagagca tatgatgtat aaaaaaataa
     3601 aaatgttagg acggaaatta cgttgcgagt acaatatatt aagagggttt atatatgatt
     3661 ataaaatata caacaacgct tcactaaaaa cacataaaaa aacaatgggt tttgatgaat
     3721 ctctagggtt gataatcaga ctatatcact cccttgaaaa agggttagca aatgaaaaat
     3781 tcaaaaaaga atcgtcatta aaaaacgttt cattgctgct gactatattt ttagaaaata
     3841 ataaaattga ttatagcgat gttcatgttc aagcagccat aaaaacagta tctttatatt
     3901 tcgaaaaaca tccaaataag ggaacgcttg aatttgaaat tcaaagaaga aagttcaaac
     3961 aaattttaga gaaagttggt gatgtatcag gttatatagg tggcgtaaaa caaataccaa
     4021 caaaacctaa aaatcaggaa atagattata aagaatttgt taaaacacgc gttagcgtta
     4081 gatcattttc aggaaaaaaa ataattctcg aggatatctt aaaagtcatt gatattgcaa
     4141 ggtattgtcc atctgcctgc aacaggcaag ccgttaagtt attttattct ttaaattcta
     4201 aagaaaacga gcacatacta aagctacaaa atggtagtcg ttcattccgt gaagctgttc
     4261 ctggcttaat agtaataaca tcggatttaa gatatcaaga gggtagcgaa gagcgaaatt
     4321 taggtttcat tgaaggcggt atttggattt cttcacttgt gaattcactc catgcgtata
     4381 atataggaag ttgtgtttta aattggtgtg ttaatccaga aactgataaa aagttgcgag
     4441 atttgataaa tatcccttat aactatcaga ttatttcatt acttgcaatt ggatatgcta
     4501 ataatgatca gcttgttcca ttttcagtaa gaaaagaagc tatcgatttc atacaaaaca
     4561 ttaatttgaa atgagcataa aaatgaaagc taatacgtca aatacgaaaa gactattttt
     4621 caatagtatt ataatgtatt tacgtatgat tttgttaatg ttgattacgt tatatatatc
     4681 cagggtgacc atcaacacac ttggaataga aaagtttggg ttgtatagtg ttatttctgg
     4741 atttgttctc ttagctggag tctttactaa tatattagag tcaacatcac aaagattttt
     4801 atctaagtct ttaggagaag gtggtaatgt ttcatcgatc tttgagtctt gcatcagtac
     4861 tcatatagtt ctttcattga tcgctgcatt attaatcgag atgttgggag tatggtttat
     4921 aaattataag ttaaggcaag atgtcatatc aataaatgaa gtttatataa tatttcatct
     4981 ctcagtaatt acattcgtcg ttactgtaat aacttctcca ttttatgcag ctcttatatc
     5041 attcgaaaat gttaattact atagtatctt cactttatgc gatgttatat ctaaagttgt
     5101 aggaataagt ttggcgccac aattaccatg ggaaattact attaattatg ctgctatgat
     5161 actaattaca acattggtta atagggtaat tcttactagt tgttttgtaa tgcgctatcc
     5221 taaactaggt tgtagacctt ctctagattt ttgtcaaatg aaaaatatat tatcatttgc
     5281 agtatggaat atgtggggag gagctgcgac agtattgaac tctcagggaa taaatattgt
     5341 tataaatgta ttttttggga tactttttaa ttcggcaaga gctattacat ctcagataaa
     5401 ttcggcaata ttgcaactaa caaatagtat acaacttgca ttaaatccac aaattgttaa
     5461 aagttatttt aaagataatt catgttattt aaaccagtta ataatttgct cggggaagat
     5521 taatgtatat ttaacattgt tgataatttg tattttttta ccaaacatta attttttatt
     5581 gacggtatgg cttggtaatt ttcctgtgat gacgatactt ttaataaaat taatgtttat
     5641 tgaattgttc attaatagtt tttcggcatc actttcatca ctgattcagg caacaggtaa
     5701 aatcaaaatc tatcagatgg tggttggtgg agtaatgttg ctgaacttac ctttgtgcct
     5761 attgatatta tcaatattta aagaagtcga aatagtctat cttgttgcta tctttgtggc
     5821 cttagtctgt ctggttttaa gattgatatt tgttaatatg aattctaaaa ttaatatagt
     5881 gtcatatcta actaatgtaa ttttgcctgg aggggggatt gcatttatta tttatctaat
     5941 acaaaacagg tggctaacag aagctacgga tctattaaca gctttaatta atattttata
     6001 tacactgttc ttatatagtg tgttaatcat tgtgattgga ttgaataata cggaaagatg
     6061 ttttgtttta aagcattgtg gattttttaa ttggatgaaa aatggctaaa ttaaaaacta
     6121 aatcaagtaa ttgcggaatt ataacattcc ataaagcaca taattatgga gctgttttac
     6181 aggcatatgc gttaatgaca acattaaaaa aaaatggatt acaagttagt tttatagatt
     6241 atgagaatga agtgctttca agggggtata gcttttatcc tatattaaag ggaacgtcga
     6301 ctataaatta tattaaggaa tggattcatt tgattttaga tttaaaacgt aaatataaaa
     6361 ggtttaaagc atttagtgat tttataaata aatatattgc atgtacgcca ttgaataaag
     6421 acactaaatg tttcgatatt atctttcttg gtagtgatca gatatggaat gcaaattata
     6481 ctaacggagt agaccctaac tattatggac aaggacctta ttgtaaagcg cacaaattag
     6541 tttcttatgc ggcaagtatg ggtaagctgt gtttgggaaa atatgaggaa caagcattct
     6601 tatctttgat aaataatata cagcagattg gtgttagaga aaattattta aagacgtaca
     6661 tagaagaaaa aaccgattta aaatgtgatg ttaatcttga tccgactctt ctgcttacta
     6721 aaagcgattg ggataaactg gcagcaccta acgatacaca agaacgctat cttcttatat
     6781 atgaaatgca tactcacagg agtacgaata ttattgctaa taaaatagca aaaatactaa
     6841 atttaaagat taaaaaatta gcatgtcgaa cgaattacaa aattgagaag gatgtaataa
     6901 caaatgcagg accacaaaat ttcttaactt tatttaaaaa tgcagctttc gtggtgacca
     6961 cttcttttca cggaactgta ttctcaatta taaatcaggt gccattcttt actttggaat
     7021 ttggtaacga gatagactta agaagccgtt cacttcttga aatgcttaat ttgaatgaac
     7081 gaatgatcag tgacgatgca aatttgaatt atgagaagct tttcttggaa tttgatgagg
     7141 ctcattcaat attagaaagt aaaaggcagg attctttaag tttcattgag agagctctga
     7201 gttaaataaa atgactaaag ttttaatagt gttccatgac ggtgataaaa aaagtggtgc
     7261 aacagcctca atgttagatc ttttaacaga gctgatcaaa tgtaaagatt tgaaaataat
     7321 atgcttaatt ccaaagtatg ggtctttata tgatgaacta aaaagattaa gaattaaaac
     7381 ttatgttatt aaatattata gtgggagata tagtcttggg agttataaag gtactgtttg
     7441 gaatattata aaaacctcaa taaaacagtt aattaccttt ttatctttta tgaaagttaa
     7501 aaataaattt atgaatatcg atgtcgttat acgaatacat ctgataacta tatgggattg
     7561 ttactctcaa tttttcttaa aaaaaagaat atttttcata ttagggaatt cggtttagaa
     7621 gatcaacacc aaaagcatat aattacagat catttatatt actcgttggt aaacaaatat
     7681 gctaatgaag ttatagttat atcagaagcg ttaaaaaata aaataataaa atacattaca
     7741 ggaaataatc ttaatctaat atatgatgat gtccatatcc aaaacaaacc aatgctaaat
     7801 tatgctaatt cggctaggct gcggaagttc attattattg gcacattatg tgaggggaaa
     7861 ggtcaaaaaa tagccataga ggctatgcat aatctgattc gtgaaggata tttatgtcac
     7921 ttaaaaataa ttggaaataa tagagttcca tatgcaagtt atcttaataa aattgttgct
     7981 gattataatt tgagtgatta tgtagagttt atgggattta ggcatgatct tgatcaaata
     8041 aggcttgaca acgatgtttg cttaattccc tctctttctg aagcttttgg tagagttacc
     8101 attgaatcaa tggctgcagg tatgatagtt gttgctagtg attctggtgc tagtaaagag
     8161 atcatcaatg atggtataaa cggttttttg ttttcttcag gttcggtaag tgatcttact
     8221 agcgtactta aaaaaatcct tgatgtagag tctaataatt tagaatgtat aaggaaaagg
     8281 gctttggttg actcacaaaa atatacatca ggacatgcag cttcatcaat ttataatttg
     8341 atcattaact gattttttat aaactttgat tatcattttt aaatgttaat taaaaaaaca
     8401 ataagggcta attagtgcaa agtaatgtta aagggttgag ctattattgg tcttttctat
     8461 gttttacaat aatgatattt ttccttttca gacaataccc attattaaag tattcatcaa
     8521 ttatgaatgt gtcttgtctg ttatttctca tatttgtcac tttttttata aaaagaggag
     8581 tatttagttc tgggattttt ttattttccc tcatattttt atcagttcat gggttgtatt
     8641 cactttattc gggtaacgac cctgtgttga tatcgcgatt ttatataatt cttattttaa
     8701 ttattgtcag ttattatgca gcaataaagt caatttctta tattaatatt ttcatctttc
     8761 tggccgtgac tcaggctttc gtaataattg gattagaagc ttttatgttt ctaaatttta
     8821 ggttttccga ttactctgca atcagaaatt actttattga taatcagttt ggtgatatat
     8881 atacatataa tggatttttt taccatattc aaattaaagg gaatgcatta cttttgtttt
     8941 cttatatgat atctttttat ctatataata aaacaaataa taaattatat cttctttcat
     9001 ccatgttcct tgcattagca gtcttatttt gtgggaatct tgctttttat attacttttc
     9061 ttattcatgc ttttattttt ttgtttttaa gaaaagcaag gacatataat cagttattat
     9121 taaaggtgtt tattttttta attacattcg gcgtttttat ctcgtattct ggaatggaat
     9181 acttagttaa agcatatgaa cttaaatttc aaggtgccaa cttttcatct atgggcacga
     9241 gatttgacca attcaatgta ttaattgatg atttgtttga aaatgtatta acagctcttg
     9301 tggggcaagg gttaggtaac attattaatg tacaaactgc cgtgcgtaat tactcagatt
     9361 atatatacta tgaattacaa gctgtatatt ttctgaatca gttgggtgtt cttttatttt
     9421 tattgtttgt tatcattaac gttattttga cacttaagtt tattaaacct atagagctta
     9481 gaatagttta tatgctatat attttatacg cattagtgaa tccttatatg ttggacacga
     9541 atcatgttgt tgtagttttt attttagtca gtttgagctc tattttttcg aaaggcggag
     9601 attgttctta taatggaaaa aaacatatca gtagttataa ttacatttaa tcccaaagtg
     9661 gaaatattga aggaaaatat aattaatctt ctaaaatcat caattgttat caaagttata
     9721 attgttgata atggtgcatc aaaaagttta gacgaattaa aaagtattcc agatgttata
     9781 attaagcacc ttggtcaaaa ccgtggtatt gcaacggcac aaaatgtcgg gctaagaatt
     9841 tctcaatctt tatgctcaaa gtatgcgcta ttactggatc aagattcaga aattgaaaca
     9901 gatttagtgt caaaacttct cgacggttac attacattag aacaaaaagg catcaaactt
     9961 gccgctgttg gtccccgacc gtttgattta tttgaaaaca aaaaaatgaa agcaagcatt
    10021 caaagagata ttcaaatatt tgagggtttt tctttgacga aacagataat cgcttcaggt
    10081 aagctaatta atttggacgt tttagatcaa gtcggtttta tggaagatga tttatttatt
    10141 gatggtgttg atcatgaatg gtgttggcgt gctggaagat taggatataa aattgcaata
    10201 atagaaaatg ctattatgaa acatcgatta ggcgacgcac gtggtaattt tttaggattt
    10261 acttataaaa ttggttcgcc tattagacta tattatcaat ttagaaacat cttagttcta
    10321 agtcgaaggg attatgttcc tttgtattgg aaattaagaa atttgatagg tatgtttctg
    10381 aaatttatta tattttctct aaaagagaat gattcaaaaa ttagacgcta ttatatgatt
    10441 aaaggcttct gttctggtct gaagaaagat aaaggtatca tcgataaacc atttaaataa
    10501 tgcgatagat tctcattgat atttataggt tgcattagga ttatgttgaa tgttcaaatt
    10561 ttcagtttta atgtctgtgt attataaaga aaaaccagct tatcttgatg aggcgttgaa
    10621 tagtcttgcc atacaaacct ataaggctga tgaaatagtt ttggtgattg atggtcaaat
    10681 cccaattcaa ttaaaaaatg ttatagataa gtggttgcta ttattaccta taaaaacaat
    10741 cccactggag aaaaatgttg ggcttggtaa tgcgcttaat attggattaa attatgtcac
    10801 taataaatta gttgctagga tggatactga tgatatctgt gttcctgaaa ggtttgagaa
    10861 acaaataagt tattttcaga aaaatccaag tacgattata ttaggtgggg ccattgatga
    10921 atatgatgag acttttaaag ataggagagg gcgtagattt tcatgttgct cgcacagtga
    10981 tatagtgagc tattctctct ttagaaatcc attcaaccat atgactgtaa tgttcaataa
    11041 agaatttatc ttgggaatag gtggttacaa gcatcatcat tacatggaag attataattt
    11101 atggctaagg tgcattgcta atggtgccat atgttctaat attgataata cgctggtcct
    11161 tgcacgaaca ggtgatgcaa tgatagaacg aaggagggga agggattatt taaaaagtga
    11221 aatcgaattg gcacgcttga aaattaagtg ttttccaaat aaaaaagcaa aaatcatttt
    11281 aataactttt tctagactta tagtacgtat actacctcag caaatattgt catcagtgta
    11341 taaaaatatc aggaaaaaat agtaagttat tttctacatg atttgcggtc cttgctataa
    11401 gagtgacatc aatttttcgt gtttatatct ttcgtatata cattactttg attttatgtg
    11461 attattgttg gggatagtaa taataaaaca atgcgttgct tatttttgtt ggtttgattt
    11521 tttgtgtgaa atttaaagca ttaggcatct ttcttctttt tttattgaaa aaagtttttt
    11581 tgttaatttg ataattatta gattgatttt tttgttttat tatgtatctc aattaattat
    11641 tgagaagttg ttgttttata gggtggcgta tttaatttat acggcttact aatttaatga
    11701 atagaactca attgtatgtc catttgattc agtctgccga caactttgtt tttaccgtca
    11761 atatcatata tataatactt tatttcaagt aaacttttta agttattctc ataccgcaca
    11821 cgcggtaaaa ccctgacagg agtaaacaat gtcaaagcaa cagatcggcg tcgtcggtat
    11881 ggcagtga
//

</pre>