<pre>LOCUS       KJ755552               11699 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain CDC 149-51 serotype O140:K-:H43 O-antigen
            gene cluster, complete sequence.
ACCESSION   KJ755552
VERSION     KJ755552.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11699)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11699)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Baranzoni,G.M.,
            Liu,Y. and Debroy,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: Average 50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CDC 149-51"
                     /serotype="O140:K-:H43"
                     /db_xref="taxon:562"
     misc_feature    1..11699
                     /note="O-antigen gene cluster"
     gene            12..1325
                     /gene="wzx"
     CDS             12..1325
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AIG62472.1"
                     /translation="MQCSGSCKARGGSVDNFIYGEALAFNTNLNKNIAYLFLVQGSSY
                     LLPLITFPYLVRVLGPEFFGVLGFCQASMQYLVMLTEYGFNWTATQKIAINKNDRNKL
                     THIFWGVIFAKILLSFFAFIMLTLVCIFIERYRDVALILAAFSPLVFGNIIYPIWFFQ
                     GLEKMKWISLCTITARAMLIPLTFIFVKSPEDVWIAALIQGAINLFAGCISLYIIKRN
                     KWVGRVVFSVKDIKENLVDGWHVFVSTSAISLYTTSTTIILGFVSGPIAVGYFNAANT
                     IRNAAQGLLNPLTQAIYPRINSLFDTNYPQALSLIKKSLRYIGLLSLLGSSFLFILAP
                     YIISLTVGEKYLYSVDILRWMAFLPFVLVLSNIFGVQTMLTHDYKKEFSYILILSGVL
                     NLTIIFPLVYKYSAIGAAISILITESFVALAMYLFLRIKNIYIIK"
     gene            1337..2431
                     /gene="glf"
     CDS             1337..2431
                     /gene="glf"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="AIG62473.1"
                     /translation="MYDFIIVGAGLFGAVCANELSKKNKKVLVLEKRNHIAGNAYTEN
                     DEGIQVHKYGAHIFHTNDKEVWEYVNLYAEFNRFTNSPLAFSGGKLFNLPFNMNTFYQ
                     LWGAQTPQEAMSIIDEQRREIGEKIPTNLEEKAISLVGRDLYEKLIKGYTEKQWGRKA
                     SELPAFIINRIPVRMTFDNNYFTDRFQGIPVGGYTKMVERMLDGIEVRLNTDYLKNKS
                     NFRKMGTVVIYTGPIDEYFEYCYGMLEYRSLRFETSTLPVSNYQGNAVINYSERKVPY
                     TRIIEHKHFDPVDTPITKITKEYPCEWKEGDEPYYPINDEKNMLLFNKYKKLSKNEDE
                     VYFGGRLAEYKYYDMHQVIRAALNLSRKWS"
     CDS             2435..3427
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AIG62474.1"
                     /translation="MKYTSVHVCCPGNIISGGPELLHQFVHELRQNGVDAHIIYFPFE
                     QLYDTPALYSHYDIAKSNINEIKKEDIVFIPETATYLINRISCENIAIWWLSIDFYYG
                     CPVDKKISPLMLKHYVDVWKGKKLSFSEMKKFQHFHQSYYAKDFLSRKGIISKELSDY
                     LNDEFIKTNILNRDNNRKKIIAYNPKKGIKTTNRLIKAFPNYKFVPLINMRPNEIKNL
                     LKSIMIYIDFGHHPGKDRFPREAAISGCCIITGQRGSAKNNVDLPIPDRFKLRDNSND
                     LEVKFGELVESIFDCFEKETMSFEDYRTKIRQEHQIFQRNVYEIAKSCAITKEI"
     gene            3472..4557
                     /gene="rmlB"
     CDS             3472..4557
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="AIG62475.1"
                     /translation="MKIIVTGGGGFIGTALIHYIIYNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGNKKNSFRFHHISTDEVYGDLPHPDEVNNKEGLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLIILNALEG
                     KTLPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            4557..5456
                     /gene="rmlD"
     CDS             4557..5456
                     /gene="rmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="AIG62476.1"
                     /translation="MNILLFGKTGQVGWELQRSLAPLGNLIALDIHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGTSEIPWQETDATAPLNVYGVTKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTSAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            5514..6389
                     /gene="rmlA"
     CDS             5514..6389
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase 1"
                     /protein_id="AIG62477.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKEGGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGGLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAFRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKG"
     gene            6397..6930
                     /gene="rmlC"
     CDS             6397..6930
                     /gene="rmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="AIG62478.1"
                     /translation="MNVIRTEIPDVLIFEPKVFGDERGFFFESFNQKIFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSEVAHFVYKTTAYYSPKFERGICWKDPFLSIEWPEI
                     GEVLMSFKDKELPSLII"
     CDS             6997..7965
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AIG62479.1"
                     /translation="MNIIIRSDFFLAKLLLNNLPPKYIIYGVEELIDINEKLNRTFLH
                     KLIKYRLIPVFKLTLCNLLRSCFKKRNKDKVFYFDIDRIDILASLILRNWDADKQIIW
                     LWNPTAKVMKSTTQLLFFINLIKKNKVEIWTFDQNDASLYGLKYYNQIYSLAMVDLFK
                     QTNNSIDVLFVGQDKERLQLLLMIKQKMDREKLVTYFHVVKDGGVTYTRDEKAFLSDE
                     ILSYEDYIHKVQQAKCLLDINQSGQTGLTLRVLEALFFNKKLITNNPNVVKYNFYNSN
                     NIMIIEDKVDNVSFNEFINSSYQEIEQSIKNEYDVEFMLDTIFQTS"
     gene            7988..9178
                     /gene="wzy"
     CDS             7988..9178
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AIG62480.1"
                     /translation="MHRNITLKLILACLTTFVMMLFFPLWLNLLIFTIILFISDNSLK
                     IPVSVILALLSSLFISSVDIAYDLKNYHDLFVNINSDIYTSEFKFEPFLLYYYSVLSQ
                     LDVDFRVVLFLQSLFLNVTLLYVFIKTLGIKGIKYYGLLLIFPQYVQLSLFLSRQTIS
                     LLFVVLLITYCLNNKMTLFKFIVVSTIAVSAHAISLLYITIILLSSRLKKYLTWKMLF
                     TIWLVGILMPITADIMNNILQNVFNQIPLLDRKIGFYMNSVSTNISASLTLYSISILP
                     LHILFFMILLSIKKKKYKTTATAFTFVYLIVYYLIIFLINYPLLPARIGLLIIIFTPY
                     FYFVAINVFYSDSKYYKVMENFGVLFVLAIFIRFLFTNDYGDYNVNLANKHILEYSLS
                     NLLN"
     gene            9189..9965
                     /gene="weoF"
     CDS             9189..9965
                     /gene="weoF"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AIG62481.1"
                     /translation="MKYIVGSGWWCNEKEDYREKCYGDNSIRGKEFHQKWYESLLLNT
                     SPAEIYIVDSDSPIKPNFDKRIKFVSLNENGGHSTNLTGKYSGWMRSVIHSMSYAQCC
                     DCDYYIYVEQDVLLKGKGIVEYCISKMTKPFMFGLCHDHHNPLQQSFFIIKKDYIDIF
                     LSRLYLIKYNDFDISPEKKFALATSPFFHLIPQILFVRPKSKLMLRLFWRIQNTLAKI
                     LGSYDYLPIGYGRDRPINLSDKYYYFQHGTMEEINDYYIQ"
     CDS             9925..10812
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AIG62482.1"
                     /translation="MVQWKKLMTIIFNKIIFLLKCLFSTIKLHLRAIFCRLNRNQCVS
                     QNSEYIITLTSYHERFNTLYLTLESLFNQSVKPSNIYLWISQEDIDKYGGLPDSLPRY
                     INAGLTVKILKENIRSYKKLSYIEDVLKKDNNIKFIITADDDIMYPRRWAQGLIQTTK
                     KHGSVSCYRGHNLTYAEGSYNYNQSINQNKFSVEPSFDLLPTGCSGICYLRKSINKLV
                     NDRRFLNFAYDADDIWYKAMTLSAGFKCVRVEPRNIHFPLIITCLSNALYSKNVWQNE
                     NDKKLIETFSLLNVEDFFK"
     gene            10809..11567
                     /gene="wfgD"
     CDS             10809..11567
                     /gene="wfgD"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-Glc:alpha-D-GlcNAc-diphosphoundecaprenol
                     beta-1,3-glucosyltransferase"
                     /protein_id="AIG62483.1"
                     /translation="MNDLHFDDLVSIIMPTFNSIKTVKDSIKSVLEQSYLNWELLITD
                     DCSTDGTYEYLLSLDDERIRVFRNKINSGAGASRNISIKNAKGRYIAFLDSDDLWHKD
                     KLYIQISFMKSKNLGLCYSGYQKIGMNGALGKKIQPPEFVNYHQLMKSNVIGCLTAVY
                     DQNIVGKVFMPTIRKRQDMALWLKILETIDYAECAPGILAYYREGHESLSSNKIKILS
                     SQWSFYRKYMKLNLYQAIYYFSFYVTKALMKHKI"
ORIGIN      
        1 cgtaggcatg catgcagtgc tctggtagct gtaaagccag gggcggtagc gtggataatt
       61 ttatttatgg tgaagctttg gcttttaata caaatcttaa taaaaatata gcttatcttt
      121 tcttagtaca aggaagttct tatttactac ctctcataac tttcccatat cttgttcgtg
      181 tattgggacc agagtttttt ggggtgctgg gtttctgcca agcatcaatg caatatttgg
      241 tgatgcttac agaatatggt tttaattgga ctgcaactca aaaaatagcc attaataaaa
      301 atgatcgtaa taagttaaca catatttttt ggggggttat ctttgcaaaa atactattaa
      361 gtttttttgc ttttattatg ttaacattag tttgcatttt tattgagcgc tatcgcgatg
      421 ttgcattgat acttgcggcg tttagccctt tggtttttgg taatataatt tatccaattt
      481 ggttttttca aggattagag aagatgaaat ggatttcatt gtgtacaata acggcacgag
      541 caatgttaat tccattaacg ttcatttttg taaaatcacc agaagatgtt tggatagccg
      601 cgttaattca gggggcaata aatttatttg caggttgcat cagcttatat ataatcaaac
      661 gcaataagtg ggttggtaga gttgtttttt ctgtaaaaga tattaaggaa aatttagtag
      721 atggttggca tgtatttgtt tcaacatctg ctataagtct ttatacaaca agcacaacta
      781 taattttagg atttgtttct ggtccaattg ctgtggggta ttttaatgca gcgaatacaa
      841 tacgtaatgc tgcacaaggc ttactcaatc cgctaactca ggctatttat ccacggataa
      901 attctttatt tgatacaaac tatccgcagg cattaagttt aattaaaaaa tctttacgat
      961 atataggttt attatctttg ctcggatcat cttttttatt catattggct ccgtatatta
     1021 tttcattaac agtaggagaa aagtatttat actcagttga tatattgagg tggatggcct
     1081 tcttaccatt tgtacttgtt ttaagtaata tttttggtgt tcaaactatg ttgacacatg
     1141 attataaaaa ggagtttagc tacattctta tattgagtgg tgttcttaat ctaacaatta
     1201 tctttccctt ggtttataaa tattctgcaa taggtgcagc catttctatt ttaattactg
     1261 aatcctttgt ggcgttggca atgtatttgt ttttgcgaat aaagaatata tatattatta
     1321 aatgagtggt gaatcaatgt atgacttcat tatagttggt gctggtttgt ttggggctgt
     1381 ttgtgccaac gaattgagta aaaaaaataa aaaagttcta gttttagaaa agagaaacca
     1441 tatagctggg aatgcctaca cggaaaacga tgaagggatt caagtccata agtatggagc
     1501 gcatatattc catactaatg ataaagaagt gtgggagtat gtaaatttat atgctgaatt
     1561 taatcgtttc actaattcgc ctttagcttt tagcggaggt aagttattca atttaccttt
     1621 caacatgaat actttttacc aactctgggg agctcagacc cctcaggaag ctatgagtat
     1681 tatagatgaa cagcgaagag agattggaga aaaaatacct acaaatttag aggaaaaagc
     1741 aatatcacta gtggggcgtg atttatatga aaaattaata aaaggttata ctgagaaaca
     1801 atggggaaga aaagcttctg agttaccagc atttataatt aacagaatcc ctgtgcgtat
     1861 gacatttgac aataactatt tcacagatcg cttccagggt atcccggttg gtggatacac
     1921 taaaatggtt gagcgtatgt tggatggtat tgaagtacga cttaatacag attatctgaa
     1981 aaataagagc aattttagaa aaatgggaac agttgtaatt tatactggac cgattgatga
     2041 atattttgaa tactgctacg gtatgctcga atatcgttca cttagatttg aaacctcaac
     2101 acttcctgta agtaattatc aaggaaatgc ggtaattaat tattcagaaa gaaaggtgcc
     2161 ttacacaaga atcattgaac ataaacattt tgaccctgtc gatacaccta taacaaaaat
     2221 aacaaaagaa tatccatgtg aatggaaaga aggagatgaa ccttattacc ctataaatga
     2281 tgaaaagaat atgctactct ttaataaata taaaaaacta tctaaaaatg aggacgaagt
     2341 gtattttggc ggtcgtttgg ctgagtataa atattatgat atgcatcaag tcattcgtgc
     2401 agcattaaat ttatctagaa aatggagttg atagatgaaa tatacgtcag ttcatgtgtg
     2461 ttgtcctggt aatattataa gtggtggtcc tgaattatta catcaatttg ttcatgagtt
     2521 aaggcagaat ggcgttgatg ctcatataat ttattttcca tttgaacagt tgtatgatac
     2581 tcctgcgtta tatagtcatt atgatattgc caaaagtaat attaatgaaa taaaaaaaga
     2641 agatattgta tttataccag aaaccgcgac atacctaata aataggatct cttgtgaaaa
     2701 tattgctatt tggtggcttt caatagattt ttattatgga tgtcctgtag ataaaaaaat
     2761 ctctccactg atgcttaaac attatgtcga tgtgtggaaa ggaaaaaaat tgtcatttag
     2821 tgaaatgaag aagtttcaac attttcatca aagctactat gcaaaagatt ttttaagtcg
     2881 aaaaggtata atttctaaag aattatctga ttatttaaat gacgagttta ttaaaactaa
     2941 tatattaaat agggacaaca atcgaaaaaa aataatagca tacaatccta aaaaggggat
     3001 aaaaaccaca aataggttaa ttaaggcatt tcccaattac aaatttgtac ctttgataaa
     3061 tatgaggcct aatgaaataa agaatttatt aaagagtata atgatttata ttgattttgg
     3121 acatcatccg gggaaagatc gttttcctag agaagcagct atttcaggat gttgtattat
     3181 tacagggcaa cgggggagcg cgaaaaataa tgttgatcta ccaattccag atagatttaa
     3241 attaagagat aatagtaatg accttgaagt gaaatttggt gagcttgtag aatcaatatt
     3301 tgattgtttt gaaaaagaaa caatgagctt tgaggattac aggactaaaa ttagacaaga
     3361 gcatcaaata tttcaacgaa atgtatatga aatagcaaaa agttgtgcta tcacgaaaga
     3421 gatataattt tatataaacc taaatgattt gtctgtaagg tattcgagag catgaaaata
     3481 attgtgacgg gtggaggcgg ttttatcgga actgctttga ttcattatat tatatataat
     3541 acgcaggata gtgttgttaa tgtcgataaa ttaacgtacg ccggaaacct ggagtcactt
     3601 gcagatgttt ctgattctga acgatatgtt tttgaacatg cggatatttg cgatgcagct
     3661 gcaatggcac ggatttttgc tcagcatcag ccggatgcag tgatgcacct ggctgctgaa
     3721 agccatgtgg atcgttcaat tacaggccct gcggcattta ttgaaaccaa tattgttggc
     3781 acttatgtcc ttttggaagc ggctcgcaat tactggtctg ctcttgatgg caacaagaaa
     3841 aatagcttcc gttttcatca tatttctact gacgaagtct atggtgattt gcctcatcct
     3901 gacgaggtaa ataataaaga aggattaccc ttatttactg agacgacagc ttacgcgcca
     3961 agcagccctt attccgcatc aaaagcatcc agcgatcatt tagtccgtgc gtggaaacgt
     4021 acctatggtt taccgaccat tgtgactaat tgctctaaca attatggtcc ttatcatttc
     4081 ccggaaaaat tgattccatt gattattctc aatgctctgg aaggtaaaac attacctatt
     4141 tatggtaaag gggatcaaat tcgcgactgg ttgtatgttg aagatcatgc gcgtgcgtta
     4201 tataccgtcg taaccgaagg taaagcgggt gaaacttata acattggtgg acacaacgaa
     4261 aagaaaaaca tcgatgtagt gctcactata tgtgatttgt tggatgagat tgtaccgaaa
     4321 gagaaatctt accgcgagca aattacttat gttgccgatc gtccgggaca cgatcgccgt
     4381 tatgcgattg atgctgagaa gattggtcgt gaattgggtt ggaaaccaca ggaaacgttt
     4441 gagagcggga ttcgtaaaac ggtggaatgg tacctgtcca atacaaaatg ggttgataat
     4501 gtgaaaagtg gtgcctatca atcgtggatt gaacagaact atgagggccg ccagtaatga
     4561 atatcctcct tttcggcaaa acagggcagg taggttggga actacagcgt tctcttgctc
     4621 ctctgggtaa cttgattgct cttgatatcc actccactga ttattgtggt gattttagta
     4681 atcctgaagg tgtagctgaa acagtaagaa gcattcggcc tgatattatt gtcaacgcag
     4741 ccgctcacac cgcagtagac aaagcagaat cagaaccgga gtttgcacaa ttacttaacg
     4801 cgacgagtgt cgaagcgatc gcgaaagcag ccaatgaggt cggtgcctgg gttattcact
     4861 actctactga ctacgtattt ccgggaacca gtgaaatacc atggcaggag acggatgcaa
     4921 cagcaccgct aaatgtttac ggtgtaacca agttagctgg agaaaaagca ttacaagagc
     4981 attgtgcgaa gcaccttatt ttccggacca gctgggtcta tgcaggtaaa ggaaataact
     5041 tcgccaaaac gatgttacgt ctggcaaaag agcgtgaaga attagcggtt attaacgatc
     5101 agtttggtgc accaacaggt gctgagctgc tggctgattg tacggcgcat gccattcgtg
     5161 tggcattgaa taagccagaa gtcgcaggtt tgtaccatct ggtagccagt ggtaccacaa
     5221 cctggcacga ttatgctgcg cttgtttttg aagaggcgcg caaagcaggc attccccttg
     5281 cgctcaacaa gctcaacgca gtaccaacat cagcctatcc tacaccagct cgtcgtccac
     5341 ataactctcg ccttaataca gaaaaatttc agcagaactt tgcgcttgtc ttgcctgact
     5401 ggcaggttgg cgtgaaacga atgctcaacg aattgtttac gactacagca atttaatagt
     5461 ttttgcatct tgtccgtgat gatggagcaa gatgaattaa aaggaatgat gaaatgaaaa
     5521 cgcgtaaagg tattatttta gcgggtggtt ctggtactcg tctttatcct gtgacaatgg
     5581 ctgtcagtaa acagctatta cctatttatg ataagccgat gatctattat ccgctctcta
     5641 cactgatgtt ggcgggtatt cgcgatatat tgattatcag tacgccacag gatactcctc
     5701 gttttcaaca actactgggt gatggtagcc agtgggggct aaatcttcag tacaaagtgc
     5761 aaccgagtcc agatggtctt gcgcaggctt ttatcatcgg tgaagaattt attggtggtg
     5821 atgattgtgc tttggttctt ggtgataata tcttctacgg tcacgatcta ccgaagttaa
     5881 tggatgtagc tgttaacaaa gaaggtggtg caacggtatt tgcctatcac gttaatgatc
     5941 ctgaacgcta cggtgtcgtt gagtttgata aaaacggtac ggcaatcagc ctggaagaaa
     6001 aaccgctaca accaaaaagt aattatgcgg taaccgggct ttatttctac gataacgacg
     6061 ttgtcgaaat ggcgaaaaac cttaagcctt ctgcccgtgg tgaactggaa attaccgata
     6121 ttaaccgtat ttatatggaa caaggggggt tatccgttgc catgatggga cgtggttatg
     6181 catggctgga cacgggtaca catcaaagtc ttattgaagc aagcaacttc attgcaacaa
     6241 ttgaagaacg tcaaggtctg aaagtttcct gcccagaaga aattgctttc cgtaaagggt
     6301 ttattgatgc tgagcaggtg aaagtattag ctgaaccgct gaagaaaaat gcttatggtc
     6361 agtatctgct gaaaatgatc aaaggttagt aataaaatga acgtaattag aacagaaatt
     6421 ccagacgtac tgatttttga accgaaagta tttggtgatg agcgtggttt cttttttgag
     6481 agctttaatc agaagatttt tgaggaagct gtaggccgca aagttgaatt tgttcaggat
     6541 aaccattcga agtctagcaa aggtgtttta cgtggactac attatcagtt agaaccttac
     6601 gcacaaggaa aattagtgcg ctgtgttgtc ggcgaagttt ttgatgtggc agttgatatt
     6661 cgcaaatctt cacctacctt tggtaaatgg gtgggggtaa atttgtctgc tgagaataaa
     6721 cgtcagctat ggatcccgga aggttttgct catggatttt tagtactgtc agaggttgcc
     6781 cattttgtgt ataaaactac ggcgtactat agtcctaagt ttgaaagagg aatttgctgg
     6841 aaagatcctt ttttatccat cgaatggcct gaaattgggg aagtgttaat gtcttttaaa
     6901 gataaagagt taccatcgtt aataatctaa tgtcaagatt aataaaaaac gagcacaaat
     6961 agttaattat ctatattcca agtaacttgg gttaaaatga atattatcat aagaagtgat
     7021 tttttcttgg cgaaattgct attaaataat ctccccccta aatatattat ttatggagtt
     7081 gaagaactta tagatattaa tgagaaatta aacagaacat tcttacataa attaattaag
     7141 tacagattaa ttcctgtatt caaattaaca ttatgcaatc tcttgcgatc ctgttttaaa
     7201 aagcgaaaca aggataaggt tttttatttt gatattgata gaatagatat tttagccagt
     7261 cttatcttac gcaactggga tgcagataag caaattatct ggctatggaa tccaacagct
     7321 aaagtgatga aatctacaac gcaattatta ttttttatta atcttataaa aaaaaataaa
     7381 gtcgaaatat ggacgtttga tcaaaacgat gcttctttat atgggttaaa atactataat
     7441 caaatatact ctttagcgat ggttgatttg tttaaacaaa ccaataatag tattgatgtt
     7501 ttatttgtgg ggcaggataa agagcgatta caacttttgt tgatgattaa acaaaagatg
     7561 gatagagaga aactagttac gtatttccat gtagttaaag atggaggagt tacatatacc
     7621 agagatgaga aggcttttct ttctgatgaa atattaagtt atgaagatta tattcataaa
     7681 gttcagcaag ccaagtgtct gctagatatt aatcagagtg gtcaaacagg cctaacatta
     7741 cgtgttcttg aagctctttt ttttaataag aaacttataa caaataatcc aaacgtcgta
     7801 aaatataatt tttataactc caataacata atgattatag aggataaagt tgacaacgta
     7861 tcatttaatg agttcataaa tagtagttat caagaaatag aacaatctat taaaaatgaa
     7921 tatgatgttg aatttatgct cgatacaatc tttcaaacat cgtaatttaa atacaacgtg
     7981 aataaaaatg catagaaata ttactttaaa gttgatactt gcatgtttga caacgtttgt
     8041 catgatgcta tttttcccat tgtggttaaa cctattaata ttcacaataa ttctttttat
     8101 aagcgataac tcacttaaga taccagtgtc tgtaatattg gctttgctat catctttatt
     8161 tatatcaagt gttgatattg cttatgacct aaaaaattat catgatcttt ttgtcaacat
     8221 taactcagat atttatacaa gtgagtttaa atttgaacca tttttgttgt actattactc
     8281 tgttttatct caattagacg ttgatttcag agttgtgctt tttttgcaat cactattttt
     8341 gaatgttaca cttctgtatg tttttataaa aacattgggg attaaaggta taaagtatta
     8401 tggtctattg ttgatttttc cccaatatgt acagttgtca ttgtttctta gtcgtcagac
     8461 tatttcatta ttatttgtag ttttgttaat aacgtattgt ttaaataata agatgacttt
     8521 atttaaattt atcgttgtat ctacaatagc tgtttctgcg catgcaattt ctttgctata
     8581 tataactata atcttactct catcaagatt aaaaaaatat ctcacatgga aaatgttatt
     8641 tactatctgg cttgttggta ttttaatgcc tataactgct gatatcatga ataatatttt
     8701 gcagaatgta ttcaatcaaa tccctttatt ggatagaaaa attggatttt atatgaattc
     8761 tgttagcact aatatcagtg catcattaac gctttatagc atttcaatat tacctcttca
     8821 tattttattt tttatgatac tcttatcaat aaaaaaaaag aaatataaaa caacagctac
     8881 tgcatttaca tttgtatatt tgattgtata ttatttgatt atattcctaa taaattatcc
     8941 actacttccg gctaggatcg gtttgttaat tattattttt acaccatatt tctactttgt
     9001 tgctataaat gtattttaca gtgacagtaa atactataaa gttatggaaa actttggtgt
     9061 tttgtttgtt ttagccattt ttatacgttt tctttttact aacgattatg gagattataa
     9121 cgttaatttg gctaataagc atatacttga atatagtctt tcaaatttgt taaattaaag
     9181 aggttatttt gaaatatatt gttggttctg gttggtggtg caatgaaaaa gaggactatc
     9241 gtgaaaaatg ttatggggat aattctatta gagggaaaga gtttcatcaa aaatggtatg
     9301 aatcgttgtt attgaatact agcccagcgg aaatatatat tgtcgatagt gactcaccta
     9361 taaagcctaa cttcgataaa cgaatcaagt ttgtaagttt aaatgaaaat ggggggcatt
     9421 ctactaattt gactggtaaa tattcaggat ggatgcgatc agtaatacat tcgatgtcgt
     9481 atgctcagtg ctgcgattgt gattattata tttatgttga gcaggatgtg ttgcttaaag
     9541 gtaagggaat tgttgagtat tgcatttcta aaatgacaaa accatttatg ttcggtctat
     9601 gccatgatca tcacaaccct ttgcaacaat cattttttat catcaaaaag gattacattg
     9661 atattttctt atctcggctg tatttgataa aatataatga tttcgatatt tcacctgaaa
     9721 aaaagtttgc acttgcaaca tctccttttt tccatttgat accacaaata ttatttgtga
     9781 ggcctaagtc gaaactaatg ctacgattat tttggagaat tcaaaatact ttggcaaaaa
     9841 tattagggag ctatgattat cttccgattg gatacggtcg tgacaggcca ataaacttat
     9901 cagataaata ttattatttt cagcatggta caatggaaga aattaatgac tattatattc
     9961 aataagatca tattcttatt aaagtgctta ttttccacta ttaaacttca cctacgcgct
    10021 atcttttgtc gcttaaatag aaatcaatgt gtttcgcaaa attcagagta tataataaca
    10081 ctaacatctt atcatgagcg atttaatact ctatatttaa cacttgaatc tctttttaat
    10141 caatcggtaa aaccatctaa tatatatttg tggatatcac aagaagatat cgataaatat
    10201 ggtggtctac ctgactcgct gcctagatat ataaatgcag gattgacagt taaaattctt
    10261 aaagaaaata tcagatcata taagaaactt tcatatatag aggatgtgtt aaagaaagat
    10321 aataacatca aatttataat aacagctgat gatgacataa tgtatcctcg tcgctgggcc
    10381 caagggctaa tacaaacaac caaaaaacat ggttctgtat cttgctacag aggtcataat
    10441 ttgacgtatg cagaaggtag ttataattat aatcaaagca ttaatcaaaa taaatttagt
    10501 gtagaacctt cttttgactt attaccaaca ggttgctcag gtatttgcta tttaagaaaa
    10561 tctattaaca aacttgttaa tgacagacgt tttttgaatt ttgcttatga tgctgatgat
    10621 atatggtata aagcaatgac tctttccgca ggcttcaaat gtgtcagagt ggaaccacga
    10681 aatatacatt ttcctttaat tattacttgc ttgtcgaatg cgctatattc aaagaatgtt
    10741 tggcagaatg aaaatgataa gaaattgatt gaaacttttt cgttacttaa tgtagaggat
    10801 ttttttaaat gaatgattta cattttgatg atcttgtctc aattataatg ccaacattta
    10861 attcaatcaa aacagtgaag gatagtatta aatcagttct agagcaatct tatcttaatt
    10921 gggaactatt gataacggac gattgttcaa ctgacggaac gtatgaatat cttttatctc
    10981 ttgatgatga acgtataaga gtttttagaa ataagatcaa ctctggagcc ggtgcttcga
    11041 gaaatataag tataaaaaat gctaaaggaa gatatattgc atttctagat agtgatgatt
    11101 tatggcataa agataaatta tacattcaga tctcttttat gaagtctaaa aatttaggat
    11161 tatgctattc tgggtatcaa aaaattggta tgaatggtgc tctgggaaaa aaaattcaac
    11221 cacctgaatt tgtcaattat catcaattaa tgaaatctaa tgtgatcggt tgcttgactg
    11281 ctgtctatga tcaaaatatt gttggtaaag tctttatgcc cacaataaga aagaggcagg
    11341 atatggcgct ttggttaaaa atacttgaaa cgatagatta tgctgaatgt gcaccgggaa
    11401 tactagccta ttaccgcgaa ggacatgaga gcttatcatc aaataaaatt aaaattttgt
    11461 catcgcaatg gtctttttat cgtaaatata tgaaattaaa tttatatcaa gcaatttatt
    11521 atttttcgtt ctatgttact aaagcattga tgaagcataa aatatagaat gtattagcat
    11581 agatgttctc aatagataag ctagacgtat taatagttat cagaacttgg tactaattca
    11641 ccccctgaca ggagtaaaca atgtcaaagc aacagatcgg cgtcgtcggt atggcagtg
//

</pre>