<pre>LOCUS       KJ755548               10322 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain CDC 63-57 serotype O139:H1 O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ755548
VERSION     KJ755548.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10322)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10322)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Baranzoni,G.M.,
            Liu,Y. and Debroy,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: Average 50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CDC 63-57"
                     /serotype="O139:H1"
                     /db_xref="taxon:562"
     misc_feature    1..10322
                     /note="O-antigen gene cluster"
     gene            107..1192
                     /gene="rmlB"
     CDS             107..1192
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="AIG62431.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKTSFRFHHISTDEVYGDLPHPDEVNNNEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVTDRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            1192..2091
                     /gene="rmlD"
     CDS             1192..2091
                     /gene="rmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="AIG62432.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRMALNKPEVAGLYHLVAT
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            2149..3030
                     /gene="rmlA"
     CDS             2149..3030
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase 1"
                     /protein_id="AIG62433.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFVDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGYS"
     gene            3032..3562
                     /gene="rmlC"
     CDS             3032..3562
                     /gene="rmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="AIG62434.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKIFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVYDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFVYKTTNYYHPESERGIIWDDKNINIHWPIV
                     DIPILSKKDHNNTSLL"
     gene            3889..5127
                     /gene="wzy"
     CDS             3889..5127
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AIG62435.1"
                     /translation="MSFIHILLLIILFYLPFIFLKFGLRKKITHPPTLFCAIFSFVLS
                     MAYIAHITLGFYEISYESYAIYGLGNLSFIAGGMITDALNRRNIKNSTQLVINKQEIT
                     LLKILIFTLLLYFPIAYSEFKHATPDMPLALKILRIRERGLEEQVYSTITNNMIILSS
                     CLVMYMTFIFSIKKINVYYYSLTFVLFCAYNFMTGTRAAIILISIACIFIYLLNTNKN
                     NKTSKLFLLSVGMTAIILGALVAIFMGKDGMERDESLLANLNKVVDNYFSYTVQGVIL
                     FDNYVIGREKITPNWDILSGSAEVINKITSSNIFKTDSKFSEFSHFAKDKDGNVCTIY
                     FAIYPLYGLVGVILFFLLYGTVCTILYNHGPGIVSVLVGYINATLCLNIFNEQVFINI
                     IFTIKFICFLLLIRCFERIK"
     CDS             5124..5996
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="AIG62436.1"
                     /translation="MNVGVVIVWYNPTKAQIENVYSLANTSELKICIVDNSNDENLFE
                     KKTLSSKNISYIHNSNKGGIAGAFNRGINKLFAERTMKHIFTMDQDSELSEDYFLSMW
                     KFVRDNNCEIACPNFYDRNSKTYGTFINLTPFFYKAVNNGTTTFCISSGMIFSRKAWE
                     ILGDFDERLIIDHVDTDYCLKANNHKVTILVNYEQCLNHAIGERESHRILGVTLKPNH
                     HNYIRKYYIVRNGTYLALKYFKFSKGYFNLNILRVIHETVCVILYENDKIRKLSYMLR
                     GLRDGIKGKLGAIK"
     gene            5983..7203
                     /gene="wzx"
     CDS             5983..7203
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AIG62437.1"
                     /translation="MPLSKSIYAILSISCLLLGLYIQFLFAEFYQGDFLNLFYLGVSF
                     CSVVISMSSGGLGGFLISRFSNLSETLRLDIAVFSFTIVFSIIGVGVFCALSYTSLFV
                     LKMALHQSLIIAFLFFLYLLSLALNLIFQSYSYSKKGISGPIFYEFCSLISYILVAFL
                     LVINKEDYIICCILFCVRGYFQLILYYIFCKDKIACRLINGDEVKSFFNEIKYLMSGS
                     AYYKSEPLIDRFFLVASSHNLAAYHLISQIYNAALGLWFKISVSPLVNKLTQNTTNTI
                     VFMKLFRRGLLSQCIISIVSAIILFFTPTLHYLESINIFRPIAQNADVVYLLFIFFVA
                     SLLGQVVSNAYYSIGHHKTPVIVSCITYTLFLPAKYYLVKRYGVSGLCILVSLYHTVN
                     FIILWLMFRVKDES"
     gene            7193..8251
                     /locus_tag="PROKKA_00008"
     CDS             7193..8251
                     /locus_tag="PROKKA_00008"
                     /inference="ab initio prediction:Prodigal:2.60"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="AIG62438.1"
                     /translation="MKVNNIVRIMAVTSGIKTPSSRFRIRQHIEQLRKYNLYVDEIIP
                     PIDKNKSVPFLSKISPKYYIPLYIPWQIIKILQRLPVLLKQTRYKYIWLNRELLTGIP
                     TLEVFFCKKIILDVDDAIWKNPPMGEITARFLAKKAYKIICGNNYLAEWFRRYNNNVY
                     VIPTAVDVHKFIPRCNKRKDIVLGWIGTHGNLKYLEKIYPTLLKILNSNRNVYLNIVS
                     DKRPEFCIDNSRANYIEWSAEDEVQNFQSIDIGLMPLDDNEWTRGKCSYKLLQHMACG
                     SLVVGSPVGMNKEILLEDNGAYPANTENEWMDTLSYLISNFEEIYCIQSKKARRFIEC
                     EYSADTIREKLARVMIDD"
     gene            8244..9401
                     /gene="pglA"
     CDS             8244..9401
                     /gene="pglA"
                     /codon_start=1
                     /transl_table=11
                     /product="N,
                     N'-diacetylbacillosaminyl-diphospho-undecaprenol
                     alpha-1,3-N-acetylgalactosaminyltransferase"
                     /protein_id="AIG62439.1"
                     /translation="MTKVIMVLPRYLPIIGGAEVQCSRLIREFNKTGKVNIVSIVTRR
                     VSNTLEKKEYIDNIPVTRIPPSGTGMLVEYIFCLLLIFYLLLNHKKYDVIHCHASSIF
                     GLSCSLVGFLLKKKVIIKISTNGEIGSMQSSSIKRCISRFLSKYCTYIALNSEGYSEV
                     NCYLSKAHKCIIPNGIDFNINHEKNLELATVIRKKLHNMYGEDIYICVFVGRFVTRKG
                     IREIYECGADLTANGKKIIFVLIGDSELQRDAINFEYDHLHNFYIAGRKKNVFPYLIA
                     GDLFISPSHKEGLPNTVLEALSVGKVCILSDILPHQEIAYEHPKNVILFKTGDAQELK
                     QTIINYLELNPHKSSLHSYVQIEDNVLLDKYSISCIAEQYCLLYKDKSNAS"
     gene            9391..10155
                     /gene="wbbL"
     CDS             9391..10155
                     /gene="wbbL"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="AIG62440.1"
                     /translation="MPRDDYKIYISIVSHNHGELIKNLDCIKNLSQEFIVVIKNNCYD
                     EVLLDYLKDSNAYLINEEYNKGFGYNNNVVFSYCRLQLNMQPSDYFIVLNPDLVIKNS
                     SIKELVNKMHCSNVKAATINLYLDDKLHDYDYSVRHFPRLYDFILSLVLGKNKTKIDK
                     SIITNPTEVDWCAGSFMALSSHVYQSIQGFDTGLFYVLRGYRSLFSFKEKNIGIIYYP
                     EITGIHKAQHKNRSFFRNILFGMLKVYSDLCLLKTG"
ORIGIN      
        1 ctggtagctg taaagccagg ggcggtagcg tgcattaata cctctattaa tcaacctaag
       61 agccgcttat ttcacagcat gctctgaagt aatatggaat aataaagtga agatacttgt
      121 tactggtggt gcaggattta ttggttctgc tgtagttcgt cacattataa ataatacgca
      181 agatagtgtt gttaatgtcg ataaattaac gtacgccgga aacctggaat cacttgctga
      241 tgtttctgat tctgaacgct atgtttttga acatgcggat atttgcgatg cagctgcaat
      301 ggcacggatt tttgctcagc atcagccaga tgcagtaatg cacctggctg ctgaaagcca
      361 tgttgaccgt tcaattacag gtcctgcggc atttattgaa accaatattg ttggtacata
      421 tgtccttttg gaagccgctc gcaattactg gtctgctctt gatagcgaca agaaaactag
      481 cttccgtttt catcatattt ctactgacga agtctatggt gatttgcctc atccagatga
      541 agtaaataat aacgaagaat tacccttatt tactgagacg acagcttacg cgccaagcag
      601 tccttattcc gcatcaaaag catccagcga tcatttagtt cgcgcgtgga aacgtaccta
      661 tggtttaccg accattgtga ctaactgttc gaataactac ggtccttatc actttccgga
      721 aaaattgatt ccactagtaa ttcttaatgc tctggaaggt aaggcattac ctatttatgg
      781 taaaggggat caaattcgtg actggctgta tgttgaagat catgcgcgtg cgttatatac
      841 cgtcgtaacc gaaggtaaag cgggtgaaac ttataacatt ggtggacaca acgaaaagaa
      901 aaacatcgat gtagtgctca ctatttgtga tttgttggat gagattgtcc cgaaagagaa
      961 atcttaccgc gagcaaatta cttatgttac cgatcgtccg ggacacgatc gccgttatgc
     1021 gattgatgct gagaagattg gtcgcgaatt gggttggaaa ccacaggaaa cgtttgagag
     1081 cgggattcgg aagacagtgg aatggtacct gtccaataca aaatgggttg ataatgtgaa
     1141 aagtggtgcc tatcaatcgt ggattgaaca gaactatgag ggccgccagt aatgaatatc
     1201 ctccttttcg gcaaaacagg gcaggtaggt tgggaactac agcgtgcgct ggcacctttg
     1261 ggtaatttga ttgctcttga tgttcactct actgattatt gtggtgattt tagtaaccca
     1321 gaaggtgtgg ctgaaaccgt caaaaaaatt cgtcctgacg ttattgttaa tgctgctgct
     1381 cataccgcgg tagataaggc tgagtcagaa cccgaatttg cacaattact caatgcgaca
     1441 agtgttgaag cgattgcaaa agcggctaat gaagttgggg cttgggtaat tcattactca
     1501 actgactacg tattccctgg aaatggcgac acgccatggc tggagacgga tgcaaccgca
     1561 ccgctaaatg tttacggtga aaccaagtta gccggagaaa aagcgttaca ggaacattgc
     1621 gcgaagcatc ttattttccg gaccagctgg gtatatgcag gtaaaggaaa caattttgcc
     1681 aagacaatgt tacgtctggc aaaagagcgc gaagaattag ctgtcattaa cgatcagttt
     1741 ggtgcaccaa caggtgctga actgctggct gattgtacgg cacatgcaat tcgtatggca
     1801 ctgaataaac cagaagtcgc aggcttgtac catctggtag ccactggtac cacaacctgg
     1861 cacgattatg ctgcgctggt ttttgaagag gcacgaaaag caggtattcc ccttgcactc
     1921 aacaagctca acgcagtacc aacaacagcc tatccaacac cagctcgtcg tccacataac
     1981 tctcgcctta atacagaaaa atttcagcaa aattttgcgc ttgttttgcc tgactggcag
     2041 gttggcgtga aacgaatgct caacgaatta tttacgacta cagcaattta atagtttttg
     2101 catcttgttc gtgatgatgg agcaagatga attaaaagga atgatgaaat gaaaacgcgt
     2161 aaaggtatta ttttagcggg tggttctggt actcgtcttt atcctgtgac tatggcagtc
     2221 agtaaacagc tgttacctat ttatgataaa ccgatgatct attacccgct ttctacactg
     2281 atgttagcgg gtattcgcga tattctgatt atcagtacac cacaggatac tcctcgtttt
     2341 caacaactgc tgggtgacgg gagccagtgg gggctaaatc ttcagtacaa agtgcaaccg
     2401 actccagatg gacttgcgca ggcgtttatt atcggtgaag agtttattgg tggtgatgat
     2461 tgcgctttgg ttcttggtga taatatcttc tacggccacg atctgccgaa gttaatggac
     2521 acagcagtta ataaagaaag tggcgcaacg gtatttgcct atcacgttaa tgatcctgaa
     2581 cgctacggtg tcgttgagtt tgataaaaac ggtacggcaa taagcctgga agaaaaaccg
     2641 ctacaaccaa aaagtaatta tgcggtaacc gggctttatt tctatgataa cgacgttgtc
     2701 gaaatggcga aaaaccttaa gccttctgcc cgtggtgaac tggaaattac cgatattaac
     2761 cgtatttata tggaacaggg gcgtttatcc gttgccatga tggggcgtgg ttatgcgtgg
     2821 ctggacacgg ggacacatca aagcctgatt gaggcaagca acttcattgc tactattgaa
     2881 gaacgtcagg ggctgaaagt ctcctgcccg gaagaaattg cttaccgtaa agggtttgtt
     2941 gatgctgagc aggtgaaagt attagctgaa cctctgaaaa aaaatgctta tggtcagtat
     3001 ctgctgaaaa tgattaaagg ttattcataa aatgaacgta attaaaacag aaattcctga
     3061 tgtactgatt tttgaaccga aagtttttgg tgatgagcgt ggtttctttt ttgagagctt
     3121 taaccaaaag atttttgagg aagctgtagg ccgcaaagtt gaatttgtcc aggataacca
     3181 ttcgaagtcc acgaaaggtg ttttacgcgg actgcattat cagttagaac cttatgcgca
     3241 agggaaattg gtgcgctgcg ttgttggtga ggtttatgat gttgcggttg atattcgtaa
     3301 atcgtcacct acttttggca agtgggttgg ggtgaattta tctgctgaga ataagcgcca
     3361 gttgtggatc cctgagggat ttgcacatgg ttttttggtg ctgagtgaga cggcggaatt
     3421 tgtttataag acgacgaatt attatcatcc tgagagtgaa agaggaatta tttgggatga
     3481 taaaaatata aatatccatt ggcctatagt tgatattcct atattgtcaa agaaagatca
     3541 taataacact tcattattat gattagtaaa tttgggacat gattttttaa atgccgcctt
     3601 ttaaaaggcg gtaaaaaact actgaccgga tgctttaata gaaaatataa aaaaataaat
     3661 ttcattttac aaaatttgaa tgtcaagtta gagaaataaa actgttatat gttagaaata
     3721 ctggtcacag catgtaaatt ttcttgagtc agaatgaatt atatgtgtac tatttcttaa
     3781 aaactattga cattttttag ttggaaataa aggttaaaaa taaaaaacta ttcatattga
     3841 gagttaaata ttacgtatgc taaaaatttt cctgtcagag gatacattgt gagttttatt
     3901 catatcttat tgctaattat tttattttat ttaccgttta tttttttaaa atttggttta
     3961 cgaaagaaaa taacgcatcc gccaacttta ttttgtgcaa tattttcatt tgtactatca
     4021 atggcttata tagctcacat aactttgggg ttttatgaaa tatcatatga aagctatgct
     4081 atttatggat tagggaattt atcatttatt gctgggggga tgattactga tgctttaaat
     4141 agacgaaaca taaaaaacag cactcaactt gttattaaca agcaagaaat aaccctacta
     4201 aagattctta tttttacgct attgttatat tttccaattg catatagcga gtttaaacat
     4261 gcgacacctg atatgcctct cgcgttaaaa atattacgca ttcgtgaacg aggattagaa
     4321 gagcaggtat atagtaccat aacaaacaac atgataatat tgtcgtcgtg tttagttatg
     4381 tatatgacat ttattttctc aatcaaaaag ataaatgttt attattattc tctgacattt
     4441 gtcctcttct gtgcttataa tttcatgact ggcacgaggg cagcaattat tcttatttca
     4501 atcgcgtgta tttttattta cttactaaat accaataaaa ataataaaac cagtaagctt
     4561 tttcttttat cggtcgggat gactgccata attctaggtg ctctagttgc gatttttatg
     4621 ggcaaagatg gaatggaacg tgatgaaagt ttattagcta atttaaataa ggttgttgat
     4681 aattattttt catatactgt tcaaggggtg attttatttg ataattatgt aattggaaga
     4741 gaaaagatta ctcctaattg ggatatatta tcaggctcag ctgaagtaat taataaaata
     4801 acaagctcaa acatattcaa aactgactcg aaattcagtg aattcagtca ttttgcaaaa
     4861 gataaagatg gtaatgtatg tacaatttat tttgctattt atccattata tggtttagtt
     4921 ggagtaatat tgtttttttt gttatatgga accgtatgta caatacttta taatcatggg
     4981 cctggcattg tttccgtatt agtcggatat attaacgcca ctctatgttt aaatatattt
     5041 aatgaacaag tgttcatcaa tattatattc acaattaaat ttatttgttt tctgctatta
     5101 atacgctgtt tcgagaggat aaaatgaacg ttggtgttgt gattgtttgg tataacccta
     5161 caaaggctca gatagaaaat gtttatagtc tcgcaaatac tagcgagtta aaaatatgta
     5221 tagtggataa ctcaaatgat gagaatttat ttgaaaaaaa aacgttgagc agtaaaaaca
     5281 tatcatatat tcataatagt aataaaggtg gcatcgctgg agcatttaat agaggaataa
     5341 ataaattgtt cgctgagaga actatgaaac atatttttac aatggatcag gactcagagt
     5401 taagcgaaga ctatttctta agtatgtgga agtttgttag agataataat tgcgaaatag
     5461 cttgcccaaa tttttatgac cggaattcaa aaacatatgg tacgtttatt aatttaactc
     5521 cgtttttcta taaggctgta aataatggta caacaacatt ttgtatttca tctggtatga
     5581 ttttttcccg aaaagcatgg gagatactcg gggattttga cgaaagactt ataattgatc
     5641 atgtcgatac agattattgt ttaaaagcta ataatcataa agtaacaata ttggttaatt
     5701 atgagcagtg cttaaatcat gctattgggg aacgagagag tcatcgaatt ttaggggtta
     5761 ctcttaaacc aaaccatcat aattatatca ggaaatatta tattgtaaga aatggtacat
     5821 atttggcatt aaaatatttt aaattttcga agggatactt taacctcaat attctgcgcg
     5881 taattcatga aacagtatgt gtgattctat atgaaaatga taaaataaga aaattatcgt
     5941 atatgttacg tggattacgg gatggtatta aggggaagtt aggtgccatt aagtaaaagt
     6001 atttatgcaa tcttaagtat ttcatgtttg ctgttaggat tatatattca atttctcttt
     6061 gcggaatttt atcaaggaga ttttttaaat ttattctatt tgggggttag tttttgtagt
     6121 gttgtcataa gtatgtcttc tgggggatta ggcggctttt taatttcaag attttctaat
     6181 ctttcagaaa cattacgcct tgatattgca gtgttttcat ttactattgt cttttcgata
     6241 ataggtgtgg gtgttttctg tgctctttca tatacttcat tatttgtgct taaaatggca
     6301 ctgcatcagt cattaattat tgctttttta ttttttttat atcttttatc tcttgcttta
     6361 aatctaattt ttcaatcata ctcctactcg aaaaaaggga tttcagggcc aatattttat
     6421 gagttttgta gccttatatc ttatattctg gttgcatttc ttcttgtgat taataaagaa
     6481 gattatatta tttgctgcat attgttttgt gtgcgtggtt attttcaact gattttgtac
     6541 tatatttttt gcaaggataa aatagcttgc aggctaatta acggcgacga agttaaatct
     6601 ttttttaatg aaattaaata cttaatgagt ggtagtgcat attataaatc agagccatta
     6661 attgataggt tttttcttgt tgccagttct cataatttag cagcatatca cttaattagc
     6721 caaatttata atgcagccct tggtctctgg tttaaaattt ctgtatcacc tttagttaat
     6781 aaactgactc aaaatacaac aaataccatt gtatttatga aattattccg tcgcggttta
     6841 ttaagtcaat gcataatctc tatagtatct gctataatcc ttttttttac accaacactt
     6901 cactatttag aatcgataaa tatatttcgc cccatcgctc aaaatgctga tgttgtctat
     6961 ttacttttca ttttttttgt tgcgtctctt ttggggcaag tagtttctaa tgcatactat
     7021 agtattggac accataaaac accggttata gtttcttgta taacatatac cctgtttcta
     7081 ccagcaaaat attatcttgt caaaagatac ggggtctctg gactgtgtat tctagtttct
     7141 ttatatcaca cggttaattt tattattctg tggttaatgt ttagagttaa agatgaaagt
     7201 taataatatc gtaagaatta tggcggttac ctccggaatt aaaacgccat caagcagatt
     7261 tagaattcgt caacatattg aacaattaag gaagtataat ttatatgtag atgaaataat
     7321 ccctcctatt gataaaaata agtctgtgcc atttctatca aaaattagcc ctaaatatta
     7381 tatcccttta tatatacctt ggcagataat aaaaattcta cagcgtctcc ctgttcttct
     7441 taagcaaacg aggtataaat atatatggtt aaatagagag ctgcttactg gaataccaac
     7501 attagaagta tttttctgca agaaaattat acttgatgtt gatgatgcta tctggaaaaa
     7561 tccccctatg ggagagataa cagcgagatt tttagcaaag aaggcatata aaataatatg
     7621 tgggaataat taccttgccg aatggtttag aagatataat aataatgttt atgtgatccc
     7681 tacagcagtg gatgtgcata aatttatacc tagatgtaat aagaggaaag atatagtttt
     7741 gggatggatt ggaacacatg gtaatttaaa ataccttgaa aagatttatc caactttgtt
     7801 aaaaattctg aatagcaatc gtaatgtata tttgaatatt gtttcagaca aaagacccga
     7861 gttttgtatt gataactcac gtgcaaatta cattgagtgg tcagcggaag atgaagtaca
     7921 aaacttccaa tcaatagata ttggacttat gcctcttgat gataatgaat ggacgagagg
     7981 taaatgtagc tataaattgt tacagcatat ggcctgtggt tcattagttg ttggatcgcc
     8041 ggttggtatg aataaggaaa ttctgttaga ggataatggt gcgtatccgg caaatactga
     8101 aaacgagtgg atggatacgt tatcgtactt aatatcgaat tttgaagaaa tttattgcat
     8161 tcagtctaaa aaagcaagac gtttcattga gtgtgaatat tccgcagata caattaggga
     8221 aaaattggca agggtaatga ttgatgacta aggttataat ggtgttacca aggtatttac
     8281 ccatcattgg tggggctgaa gttcaatgta gccgcctcat ccgcgaattt aataaaactg
     8341 gtaaagttaa catagtgagc attgttacaa gaagagtaag caataccttg gaaaaaaaag
     8401 aatacattga taatatacct gtaacgagaa tcccaccaag cggaactgga atgctcgttg
     8461 aatacatttt ttgcttatta ttgatattct atcttttgct taatcataag aagtatgatg
     8521 taattcattg ccacgcttcc tctatttttg gtctttcttg ttccttagtt ggatttctac
     8581 tcaaaaagaa agtgattatt aagatctcta ctaatggtga aattggctca atgcagagtt
     8641 cgtctataaa aagatgtatt tcacggtttc tatcaaaata ttgtacctat atagcgttaa
     8701 attctgaggg ctattctgaa gtaaattgtt atttatctaa agctcataaa tgtattattc
     8761 ccaatggaat tgatttcaat atcaaccatg aaaaaaattt ggaattggcc actgtgatta
     8821 gaaaaaaatt acataatatg tatggggaag atatatacat atgtgttttt gtcggtcgtt
     8881 ttgttacaag aaaagggata agggaaatat atgaatgtgg tgcagacctg actgctaatg
     8941 gtaaaaaaat catattcgtg ttaattggtg attcggaatt acaacgtgat gctatcaatt
     9001 tcgaatatga tcatcttcat aatttctata tcgctggtag gaaaaaaaat gtgttcccct
     9061 atttgattgc tggagattta ttcatttcac catcacacaa agaaggtttg ccaaacactg
     9121 tccttgaagc tctttccgtt ggcaaggttt gtattctttc agatatactt ccacatcaag
     9181 agattgctta tgaacatcct aaaaacgtca tactttttaa aacaggagac gctcaggagt
     9241 tgaagcaaac tataataaat tatctggaat taaatcctca taaatcaagt ttgcatagtt
     9301 atgtgcagat tgaggacaat gtcttattag acaaatattc tatcagttgt atagcggaac
     9361 aatattgtct actttacaag gacaagtcta atgcctcgtg atgattataa aatatatata
     9421 tctatagttt cccataatca cggtgagtta ataaagaatt tagattgtat taagaattta
     9481 tctcaagagt tcatagtggt tattaagaat aactgttatg atgaagtatt attggactat
     9541 ttaaaagata gcaatgccta tctcatcaat gaagaatata ataaaggatt tggctataat
     9601 aataatgtcg tttttagtta ttgtcgtttg caactaaata tgcaaccttc cgattatttt
     9661 atagtactta atccagattt ggtcatcaaa aactcgtcta tcaaagagtt ggttaacaaa
     9721 atgcattgca gcaatgtcaa agctgcaact attaaccttt acctagatga taaacttcat
     9781 gactatgatt attctgtgag gcattttccc cgcttatatg actttatttt atctttagta
     9841 ttaggtaaga ataaaactaa aatagacaaa tcaataatta ccaatccaac agaagtagat
     9901 tggtgtgcag gttcttttat ggctttaagt agtcatgttt atcaatccat tcaaggattt
     9961 gatacagggt tattttatgt attgcgagga tatcgatctt tgttttcgtt taaagaaaaa
    10021 aatattggaa taatttatta tcctgagatt acaggcattc acaaagctca gcataaaaat
    10081 agaagttttt ttcgaaacat tttatttggc atgttaaaag tgtattcaga tttatgtttg
    10141 ctaaaaacgg gttaattagg aataagtcca atatttgtca ctaaagattg atttgtgctt
    10201 agaaagaaaa attaaaccag agtcttttag atatctttta aagcagtata tgctctatcc
    10261 ccccctgaca ggagtaaaca atgtcaaagc aacagatcgg cgtcgtcggt atggcagtga
    10321 tg
//

</pre>