<pre>LOCUS       KJ755546               10026 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain 1111-55 serotype O136:H- O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ755546
VERSION     KJ755546.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10026)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10026)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Baranzoni,G.M.,
            Liu,Y. and Debroy,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: Average 50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="1111-55"
                     /serotype="O136:H-"
                     /db_xref="taxon:562"
     misc_feature    1..10026
                     /note="O-antigen gene cluster"
     gene            107..1105
                     /gene="pseB"
     CDS             107..1105
                     /gene="pseB"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="AIG62412.1"
                     /translation="MFDNKTILVTGGTGSFGNKFVRMTLENYNPKKIIIYSRDEMKQW
                     EMAKKFKDENRIRFFIGDVRDKDRLYRALDGVDFVVHAAATKIVPTAEYNPFECVKTN
                     INGAMNVIDACIDKGIERVVALSTDKASSPANLYGATKLASDKLFVAGNSYSGATKTR
                     FAVVRYGNVMGSRGSVIPFFLSIKENGELPITDERMTRFMITLEQGVELVWHAFKDMV
                     GGEVYVKKIPSMKVTDIATAVAPNAKQKIIGIRPGEKLHEQMISAEDSYYTYEYPEHF
                     KILPAIHNWCNSPERIKDGKKVPEGFVYESDSNAEWMSIEELRQWIDDNREKVGNI"
     CDS             1105..2259
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="AIG62413.1"
                     /translation="MKFIPYGRQDISDEDISAVVDVLKSEFLTQGPYVPKFEKTIANY
                     VNVKHAVAVNSATSALHIACLALGMKKGDWLWTSPNTFVASANCALYCGANVSFVDID
                     ARTYNMSVSALEAKLMSAKNNGTLPKVVVPVAFAGQSCEMEAIYKLSKEYGFSIIEDA
                     SHAIGGSYQGEKIGNSHFADITILSFHPVKIITTAEGGMALTNNDSLAEKMQLFRSHG
                     ITRDINHMTKVSEGDWYYQQIDLGLNYRMTEIQAALGMSQLKRIDGFVTRRHELAERY
                     KKALEGIPISLPFQAEGGYSAFHLYPITVKNSKLRKSLFDYLRNKNIGVNVHYIPVHT
                     QPYYEKLGHKVGDYPVAEDYYSRALSIPMYSALTNEDQDYVIQCIREFFK"
     gene            2256..2966
                     /gene="neuA"
     CDS             2256..2966
                     /gene="neuA"
                     /codon_start=1
                     /transl_table=11
                     /product="CMP-N,N'-diacetyllegionaminic acid synthase"
                     /protein_id="AIG62414.1"
                     /translation="MNVAIIPARGGSKRIPQKNIKMFCGKPMIAWSINAARKSGVFDR
                     IIVSTDDAEIAAVARKYGAEVPFTRPEELSNDFAATIPVIRHAVEWLVNNGCIADFVC
                     CIYATAPFIRSEDIIRGLTIIREQQADYAFTVTRYPYPIQRALKIGHENQISMFSPEM
                     FHVRSQDLEESWHDAGQFYWGTVSAWLQEKPIFSANSYSIVLPRERVQDIDTPEDWRV
                     AEWLYKTMEFKNESVSAG"
     gene            2944..4482
                     /gene="pseG"
     CDS             2944..4482
                     /gene="pseG"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,4-diacetamido-2,4,
                     6-trideoxy-beta-L-altropyranose hydrolase"
                     /protein_id="AIG62415.1"
                     /translation="MKVFLRVDSSLSIGSGHIIRCLNLAIALRNVGAECIFISKKHRG
                     NILFKIEQAEFLYQVIPTPEEYDIYVSEEKYWLNGSQNDDALQFSLLIKKQCENPDII
                     IVDHYSLDYEWELIIKRNFPEAKLIVIDDLCNRPHCCDLLIDQTYLRHEKEYTCLNIW
                     GGKILTGPKYALLDPVFSKLREQSINRKTQLEFPQRLMITMGGVDVNNITGKVLRYIE
                     NKNLKSIEKITVILGSACPHREEIEALVADSKYPINILTNVENMAELMLEHDFAIGAM
                     GGTTWERCVMALPAVNIAIANNQSTIATNFSKAGAIVLHSDNFTKNDFYNAFNRLITD
                     YHQQRDLVMSICDGQGLIRDIQEIIPCFSTDGINVTLRFATLDDINFVYQLQCEPQTR
                     KFARNPNIPSYDSHTEWMRRKLVEQNSFFYIIEHIGACGVLRLDPIEHELAQYEISIF
                     LTTASMGKGIAKAAIRRATMLHKDTVILATVLYENFASHRLFEQIGFNKISSCEYINR
                     GGNE"
     gene            4475..5524
                     /gene="wcnT"
     CDS             4475..5524
                     /gene="wcnT"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="AIG62416.1"
                     /translation="MSKYITIDGRKIGKEYSPYVIAELSANHNGDINRAFKIMEAAKL
                     AGADAIKLQTYRADTITIDCNSEDFQIHGGLWDGQTLFNLYKGAQMPWEWHKPLFEKA
                     KELGITIFSSPFDFTAVDLLEELGAPAYKIASFEAIDIPLIKYVARTGKPMIISTGMA
                     NEQEIQEAIDAAKDGGCKELVVLHCVSGYPAPAEDYNLVTILDMAERYGVITGLSDHT
                     IDNTTAIASVSLGANVIEKHVTLDRKGGGPDDSFSLEPSELRALCKDVKTAWRALGKI
                     SYNHKESEKGNVKFRRSLYVVKDVAKGEEITTQNVRSIRPGFGLAPKHLEHVLGKKFL
                     TDLPAGTALTFDIIE"
     gene            5580..6818
                     /gene="wzx"
     CDS             5580..6818
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AIG62417.1"
                     /translation="MECRTVIMLLLRGATLASKFLLVIFLAKFATYKNLADYTIIAVT
                     ISYLLFFLGFDFYTYSTREIIKKGFTKSGELLCNQLYLYISMYLILIPIVYGLNYFSV
                     LSVGVLFYFVVITEHFAQECMRIIIINNKPVKANFQFFLRSSFWIYIYIAYCYWKATY
                     SLDILLLFWLISNVCSIVYSVSEFKMINYKDDKIYRVDFKWIKKGVAIAIPLLVTTLM
                     LRGGYVTDRYILKYLSSTEVLAVYSFYSNMSNALIAFIDAAVIMIFYPKVISAYNELN
                     IEEYNKTLVLFKRNVVKVGCCSFFILSVAVPVICLFLNKSEFINSIIMFYILLTSAFI
                     YSFSLVYHYELYARHKDFILFKCTFFSFLLTVIGQYVFASLFGGIGMAISILLFSLML
                     LSTKYLSIINIKKNECFGNS"
     CDS             6867..7895
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AIG62418.1"
                     /translation="MNLYIIQNDTHFKNFIELATEGDFFIDLARFTGQDINYELFYKK
                     GVSEINLGLHDANFYLFSSYQKIKFCFNFEMIVKKIISAYRFEKIITGNDGALQKIII
                     KQALKNNSKGRVEMWLDGLISLNKNRVINSAKVFMSYIADKIGLSSYVPSVIGTSSYV
                     DTLYVMDQSVIKEYNFLMIKPKVKKIEKRLFPRHKKLISLAHNHERRAQVCKVLYLTS
                     AWSFHGHNKCQNIQYHQILNLLSNFEGNKDIDFQIRIHPRDIITNYSEIPVGVISRVK
                     SFEEDILSASIIISARSTGLFEADMIGKKVIVYDEGFEGDFMNEYFDTLPRCVDLNEI
                     NSFISQQG"
     gene            7904..9136
                     /gene="wzy"
     CDS             7904..9136
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AIG62419.1"
                     /translation="MSSTQVFSLLLGFYLVSVNLLIIPLDYFNVERSILIIPYVFIFS
                     LSLFFFALATIGDFYKSPKQIFYVQVCFVFVIIIPSCLSFIQNTLLEVVPSMMRYMSY
                     LFTFVFVYYFSAKNWFTVKNLDVSIKFISIILFIFSILQIVKGEMIYMNGAYRLSSIY
                     GTTPAGFALITLVFSVYFYSQLHVSDSGKLKKIFPFAFFMINVSMMALTQSRQSIMTL
                     LLLICIFHFVRAKNIFKFFSLIFILLLIYVFYLLVVNTDFFPRLTQMLFNYSSDSSTH
                     TRISIITQSYEHLDVNDLIYGIGLGGFNHFYYKISGEIGVAAHNDFFLFFVEGGVIAL
                     FFYCLFLCGGVSFWGRAIKQQGDSYFTPLLVFCALYVFSFLNNPYYYPQVQVVASALM
                     GVYLSEYIKKQKVNYVLP"
     gene            9123..9863
                     /gene="wfeD"
     CDS             9123..9863
                     /gene="wfeD"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-Gal:alpha-D-GlcNAc-diphosphoundecaprenol
                     beta-1,4-galactosyltransferase"
                     /protein_id="AIG62420.1"
                     /translation="MFCHELENKMKSQEFFVETLKNSQLNKTRISFLNPYSYPLISRN
                     KKIIKGFDYWFADGLTLCIVTNLFREKSDIIKRGSFDFSSLAGICFDYLQTHSLSVAI
                     IGGSNDEIHQACEYFSIRYPSLKICFKHHGYIDIDKCDHIINELKKSEVQALIVGMGT
                     PKQEEFILKVEKHIISCKLFLTCGGFISQTASSGDYYHPLIKRLGLRWLQRAVLHKHV
                     RRRLLLNYPCFYFRYICSAILNILYAKK"
ORIGIN      
        1 tctggtagct gtaaagccag gggcggtagc gtgcataaac ttagggaagt tgttacgaat
       61 tataaagatc gcagcaattt cgtattgtgt cttatctggt gaaaaaatgt ttgataataa
      121 aacgattctt gtaaccggcg gtaccggttc atttggcaat aagttcgttc gcatgacatt
      181 agaaaactat aatcctaaaa agattattat ttattctcgt gatgaaatga aacagtggga
      241 aatggcaaaa aaattcaagg atgaaaatcg gatccgcttc tttattggtg atgttcgtga
      301 taaagatcgt ctttatcgcg cattggatgg cgttgatttt gttgttcatg ctgcagcaac
      361 aaaaatcgtc cctacagccg agtataatcc ctttgaatgt gtaaaaacga atatcaatgg
      421 tgctatgaac gtgattgatg cttgtattga caaaggcatc gaacgcgtag ttgcgctttc
      481 taccgataaa gcaagtagcc ctgcgaatct gtatggtgca actaagctag catctgataa
      541 actttttgtt gcaggtaact cctattctgg cgcgacaaaa acccgttttg ctgttgttcg
      601 ctatggaaat gttatggggt ctcgtggctc agtaatacca ttcttcttat ctattaagga
      661 gaacggtgaa ctgccgataa ctgatgagcg tatgacacgc tttatgatta ctctggagca
      721 gggagttgaa ttggtttggc atgcgtttaa agatatggtc ggtggtgaag tttatgtaaa
      781 aaaaatacct tccatgaaag tcactgatat agctacagct gttgcaccta atgctaaaca
      841 aaaaataatc ggcattcgac ctggcgagaa actccacgaa caaatgatca gcgctgaaga
      901 ttcttattac acctatgaat atccagagca ttttaaaatt cttccagcta ttcacaactg
      961 gtgtaactca cctgaaagaa ttaaagatgg caaaaaagta ccagaaggtt tcgtttatga
     1021 aagtgatagt aacgcagaat ggatgagcat tgaagaactt cgtcaatgga tcgacgataa
     1081 tcgtgaaaaa gtaggtaaca tctaatgaaa tttattcctt acggacggca ggatatttct
     1141 gacgaagata taagtgctgt agtggatgta cttaagtccg agttcttgac tcaaggacca
     1201 tatgttccta agtttgagaa aacaatagct aattatgtga atgttaagca tgctgttgca
     1261 gtaaatagtg cgacgtcagc actccatatt gcttgtctgg cactggggat gaaaaaaggc
     1321 gattggcttt ggacatcacc aaatactttt gttgcttctg cgaactgtgc tctttattgt
     1381 ggcgctaatg tcagttttgt tgatatcgat gcgcgaacat ataatatgag cgttagtgcc
     1441 ttagaagcga aactcatgtc tgcgaaaaat aatggaactt tacctaaagt tgttgttcct
     1501 gtcgcatttg caggacagtc atgtgaaatg gaggcaatat ataaactctc aaaagagtat
     1561 ggattttcaa ttatcgaaga tgcttcacat gcaattggcg gaagttatca gggagaaaaa
     1621 ataggtaatt cgcactttgc agatattact atattgagct ttcatcctgt taaaattatc
     1681 acaactgcag aaggggggat ggctctaacc aataacgata gtttggcgga aaaaatgcag
     1741 ctattccgta gccatggaat tacccgtgat attaatcata tgacaaaagt aagtgaaggc
     1801 gattggtatt atcaacaaat tgatctcggt ctgaattaca gaatgaccga aattcaggca
     1861 gcgttgggta tgagtcagtt gaaacgtatt gatggctttg taacacgccg tcatgaattg
     1921 gctgagcgtt acaaaaaagc tcttgaaggt attcctattt ctctgccatt ccaggccgaa
     1981 ggcggctaca gtgcttttca cctttatcct ataactgtta aaaattccaa actgcggaaa
     2041 tcactatttg attatctacg gaataaaaat atcggggtca atgtccatta tattccagtt
     2101 catactcagc cgtattatga aaagttaggg cataaagtcg gggattatcc tgttgctgag
     2161 gactactact cccgggccct cagtataccg atgtattctg ctttaacaaa cgaagatcag
     2221 gattatgtaa tccaatgcat tcgggagttt tttaagtgaa cgtggcaatc attccagctc
     2281 gcggtggcag taaacgtatc ccgcaaaaaa atatcaaaat gttttgtggg aaaccaatga
     2341 ttgcttggtc gattaatgcc gctcgaaaga gcggcgtgtt tgatcgaatt attgtttcga
     2401 cggatgatgc agaaattgcg gctgtagcga gaaaatatgg cgctgaagtt ccgtttacgc
     2461 gcccagaaga actttcaaat gattttgctg caacaattcc tgtcatacga cacgctgttg
     2521 aatggcttgt taataatggg tgtatagcag attttgtgtg ctgtatatat gcaactgcac
     2581 catttattcg ttctgaagat attattcgtg ggttgacaat aattagggaa caacaggcag
     2641 actatgcttt tactgttact cggtaccctt atccgatcca acgcgcactc aaaatagggc
     2701 atgaaaacca gattagtatg ttttctcctg aaatgttcca tgttcgttct caggatctcg
     2761 aagaatcttg gcatgatgcc ggacagtttt actggggaac agtatctgca tggttgcaag
     2821 aaaagccaat atttagcgct aactcctatt caattgttct accgcgtgag cgtgtccaag
     2881 atattgatac tccagaagac tggcgagtag ctgagtggtt atataaaacc atggagttta
     2941 aaaatgaaag tgtttctgcg ggttgattcg tctctatcta tcggctccgg tcatattatt
     3001 agatgtttaa atttagcaat agcactaagg aacgtagggg ctgaatgtat ttttatatct
     3061 aaaaaacatc gtggcaatat tttgtttaag atcgaacagg ccgaattttt ataccaagtg
     3121 attccgacac ctgaagagta tgatatctat gtaagcgaag aaaaatattg gctcaatggt
     3181 agtcaaaatg atgatgcttt gcaattcagt ttactgataa aaaaacaatg tgaaaatcca
     3241 gatattatta tcgttgatca ttattcactc gattatgaat gggaattaat aattaagcgt
     3301 aatttccctg aagctaaact tatagttatc gatgatcttt gtaatcgccc ccactgttgt
     3361 gatctattga tagatcagac atatttacgt catgaaaaag aatatacatg cttaaacatt
     3421 tggggaggga agatattaac ggggcccaag tatgcactac tagatcctgt tttttcaaaa
     3481 ttaagggagc agtcaataaa tcgtaaaact cagctagaat ttcctcaaag gctaatgata
     3541 acaatgggag gagttgatgt aaataatata actgggaaag tcttgcgtta cattgaaaat
     3601 aaaaacctaa aaagcatcga aaaaattact gttatactcg gtagcgcgtg tccgcatcgg
     3661 gaggaaattg aggcattggt tgctgattcc aaatatccta taaacatatt aaccaatgtt
     3721 gaaaacatgg ctgaacttat gctagaacat gattttgcga ttggtgcaat gggcgggaca
     3781 acatgggaac gttgtgtaat ggctttacct gcagtgaata tcgcaattgc caataaccag
     3841 agcacgattg ccacgaattt ttctaaagct ggcgctattg ttttacattc agataatttt
     3901 actaagaatg atttttataa tgcttttaat cggttgataa ctgattatca tcaacagcgt
     3961 gatcttgtaa tgagtatatg tgatggtcaa ggactcatca gagatataca ggaaattatt
     4021 ccttgctttt ctacagatgg cataaatgtg acgcttagat ttgcaacgtt ggatgacatt
     4081 aattttgttt accaacttca gtgtgagcca caaactcgaa agtttgcacg aaacccaaat
     4141 attccttcat atgatagtca cacagaatgg atgcgtcgta aattagttga acaaaatagt
     4201 tttttctata tcattgagca tataggagcg tgtggagtat tgcgtttaga tccaatagaa
     4261 catgagttag cacaatacga aatttcgata tttttgacga cagctagtat ggggaaagga
     4321 attgcaaaag cggctatcag acgtgcaact atgttacata aagatacagt aatactggca
     4381 acggtactgt acgaaaattt tgcttctcat cgactttttg aacaaattgg tttcaataag
     4441 atctcttcat gtgaatatat taataggggg gggaatgagt aaatatataa cgattgatgg
     4501 tcggaaaatt ggtaaagaat actcaccata tgtcattgct gaattgtccg caaatcacaa
     4561 tggtgatata aatcgtgctt tcaaaattat ggaggctgct aaattagccg gcgctgatgc
     4621 aataaaatta cagacatatc gtgctgatac aattactatc gattgtaact ctgaagattt
     4681 tcaaattcat ggtggcctat gggatgggca aactttattt aatttatata aaggtgctca
     4741 aatgccctgg gaatggcata aaccattatt tgaaaaagcg aaagaactag gtattaccat
     4801 tttcagtagt ccatttgact ttactgcagt tgatctacta gaggaattag gtgcacccgc
     4861 atataagatt gcttcatttg aagcaattga tatacctctg atcaaatatg tagccagaac
     4921 aggtaaaccg atgattatct ctacaggtat ggctaatgaa caagaaattc aggaagcaat
     4981 tgatgccgcg aaagatgggg gatgtaaaga gcttgttgtg ttgcattgtg ttagcggata
     5041 tccagcacca gcggaagatt ataatttagt aacaatactt gatatggctg agagatacgg
     5101 tgtgattact gggttatccg atcacacaat agataatacc acggctattg catccgtctc
     5161 acttggagct aatgtaattg aaaaacatgt tacactggac agaaaaggag gtggacctga
     5221 tgatagtttt tctctggaac ctagtgagct aagagcactt tgcaaagacg ttaagacagc
     5281 ttggcgagca ttaggaaaaa tcagttataa ccataaggaa agtgaaaaag ggaatgtcaa
     5341 attcagacga tcactttatg ttgtaaaaga tgttgcaaaa ggtgaggaaa taacaacgca
     5401 aaatgtgcgt agtattcgcc ctggttttgg tttagctcca aaacatttag agcatgtgtt
     5461 aggaaaaaaa ttcttgacag atctacctgc aggcacagct ttaactttcg atattatcga
     5521 ataatatagt accctcttaa caaagagggt gaatcaagct cattatataa atcgatgata
     5581 tggaatgtag aacagtaata atgttgctcc tcagaggggc aacactcgca agtaagtttt
     5641 tacttgttat ttttttagct aagtttgcca cgtacaaaaa tttagccgac tatacaataa
     5701 ttgcagtcac tattagttat ttgttatttt ttttggggtt tgatttttat acatactcaa
     5761 ctagggaaat tataaaaaaa ggctttacca aaagtggtga acttttatgt aaccagttgt
     5821 atttatacat atcaatgtat ttgatattaa ttccaatagt atacgggtta aattattttt
     5881 ctgttctaag tgttggtgtt ttgttttatt ttgttgttat aacagaacat ttcgcacaag
     5941 aatgcatgag aatcattatt ataaataata aacctgtaaa agcaaatttt caattttttt
     6001 tacgctcttc attttggata tatatatata tagcatattg ttattggaaa gccacttatt
     6061 cattggatat tttgctttta ttctggttga tatcgaatgt gtgctctata gtttattctg
     6121 tcagtgaatt taaaatgatt aattataaag atgataaaat atatagagtt gatttcaaat
     6181 ggatcaagaa gggggtggca attgcaatac cattactcgt taccacatta atgctgaggg
     6241 ggggatatgt tacggacagg tatattttaa aatatctctc atctactgaa gtattagctg
     6301 tttactcatt ttatagtaac atgtctaatg ctttgattgc attcattgat gctgctgtaa
     6361 taatgatatt ttaccccaaa gtcatttctg cttataatga actaaatata gaggaatata
     6421 ataaaacatt ggtgttattt aagcgcaatg ttgtaaaggt cggttgctgt tcctttttta
     6481 ttttgagtgt agctgtacca gtaatatgtt tatttttgaa taaatcagaa tttattaatt
     6541 ctattattat gttttatata ttattaactt cagcatttat atattcattt agtctagtat
     6601 atcattatga attatatgcc agacataaag attttatatt atttaagtgt acattttttt
     6661 catttctgct tactgtcata gggcaatatg tttttgcttc tttatttggt ggcataggca
     6721 tggcgatttc aattctttta ttttcattaa tgttattatc cacaaagtat ttatctatta
     6781 tcaatattaa aaaaaatgag tgttttggca actcatagta gctaaactag aatcaacaag
     6841 taaatatcac tggtttggag taataagtga atctttatat tattcaaaat gatacgcatt
     6901 tcaaaaattt tattgaattg gctactgaag gcgatttttt tatagatctt gcaaggttta
     6961 caggtcagga tatcaattac gaattatttt ataaaaaagg agtgtcagaa attaatttag
     7021 ggttacatga tgctaatttt tatttgttta gttcttatca gaaaattaaa ttttgtttca
     7081 attttgagat gattgtaaaa aaaataatat ctgcttaccg ctttgaaaaa atcataacag
     7141 ggaatgatgg tgcattacaa aaaataataa taaaacaagc attaaagaat aatagcaaag
     7201 gaagagtaga aatgtggttg gatggtttaa tttcattgaa taaaaacaga gtaattaatt
     7261 cagcaaaagt atttatgtcg tatatcgctg acaagattgg tttgtcttct tatgtacctt
     7321 cagttatagg gacaagtagt tacgttgata cattgtatgt aatggatcaa tctgtaataa
     7381 aagagtataa ttttctaatg attaaaccaa aagttaaaaa aattgaaaag agattgtttc
     7441 ctcggcataa aaaattaata tctttggcac ataatcatga gagaagagct caggtctgta
     7501 aagtattgta tttaacaagt gcatggtcat ttcatggaca taataaatgt caaaatattc
     7561 agtatcacca gattttaaat ttgttatcta atttcgaagg taataaagat attgactttc
     7621 aaattcgaat ccatccaaga gacattataa caaactattc tgaaattccg gtgggggtta
     7681 tttctagagt aaaatcattt gaggaagata ttttatcagc tagcattatt atttctgcaa
     7741 gatctacagg gcttttcgag gctgacatga tagggaagaa agtgatagta tatgacgaag
     7801 gttttgaagg ggattttatg aatgaatatt ttgatacact tcccagatgt gtcgatttga
     7861 atgaaataaa ttcatttatt agtcaacaag gttaatttat ataatgtctt ctacacaagt
     7921 tttttcattg ctactggggt tttatttagt atcagtaaat cttttaatta tcccattaga
     7981 ttatttcaac gtagaacgct caattctgat tattccatat gtatttatat tttctctgag
     8041 tttgtttttt tttgcgttag caactattgg tgatttttat aaatcgccaa aacaaatttt
     8101 ttatgtgcaa gtttgttttg tttttgtgat aataattcca tcatgtttga gttttataca
     8161 aaatacatta ttagaagtag tgcctagcat gatgaggtac atgtcatatt tattcacatt
     8221 tgtatttgtc tactatttct ctgccaaaaa ttggtttact gttaaaaatt tagatgtgtc
     8281 catcaaattt atatcgatta tcttgttcat tttttcaata cttcaaatag taaaaggcga
     8341 gatgatatat atgaacgggg cttatcgttt gtcaagtatt tatgggacaa cgcctgctgg
     8401 ttttgcgttg attactctcg tcttttctgt ttatttctat agtcaattgc acgtttcgga
     8461 tagcggtaag ttaaaaaaaa tatttccatt tgcttttttt atgattaatg tatctatgat
     8521 ggcgttgacg caaagccgac aatcaataat gacattgtta ttgcttattt gtatatttca
     8581 ttttgttagg gcaaagaata tatttaagtt tttttcgctt atatttattt tattgttaat
     8641 ctatgtattt tatttgttgg ttgttaatac ggactttttt ccacgattaa ctcaaatgct
     8701 ttttaattac tcatcagatt catcaacaca tacacgtatt tctattatta ctcaatcgta
     8761 tgaacatctt gatgttaatg atttaattta tgggattggt ttaggcggat ttaatcactt
     8821 ctactacaag attagtggtg aaataggtgt tgcagcccat aatgattttt tccttttttt
     8881 tgttgaaggt ggcgtaatag ctttgttctt ttattgcctt tttttatgcg gtggagtgtc
     8941 attttggggg cgggcgatta aacagcaagg tgatagttat tttacaccgt tgcttgtttt
     9001 ttgtgcttta tacgtatttt cttttttgaa taatccttat tattacccac aagtgcaagt
     9061 tgttgcttct gcattgatgg gcgtgtattt gtcagaatat ataaagaaac aaaaggtaaa
     9121 ttatgttttg ccatgaactt gaaaacaaaa tgaaatctca ggaatttttt gtcgaaactc
     9181 tcaaaaatag ccagcttaat aaaaccagaa ttagttttct taacccctat tcataccctt
     9241 taatttcaag aaataaaaaa atcatcaagg gttttgatta ttggtttgca gatggtttaa
     9301 cactatgtat tgttactaat ttgtttagag agaagtctga tataataaaa agaggcagtt
     9361 ttgatttttc atcactggcg ggaatatgtt tcgattattt acaaactcat tctctttctg
     9421 ttgctattat tggtggaagt aatgatgaga ttcatcaagc atgtgaatat ttttccattc
     9481 gatatccatc cttaaaaatt tgttttaagc atcatgggta tatagatata gacaagtgtg
     9541 atcatataat aaatgagctt aaaaaatctg aagtgcaagc attaattgta ggaatgggaa
     9601 cacctaagca agaagagttt atattaaagg ttgaaaaaca tataataagt tgcaagcttt
     9661 ttttaacttg tggaggtttt atttcacaaa cagcgagtag cggtgattat tatcacccat
     9721 taatcaaaag attaggtctt cgttggctac agcgtgcagt gcttcataag catgttaggc
     9781 gcaggctttt actcaattat ccctgttttt attttagata tatatgtagt gcaatattaa
     9841 atatcttata tgctaaaaaa tagcaggata attatcaatg tagtatgcta aaattgacca
     9901 atatcgtgaa gttatcaaat gttatatcag ataacatgaa acaatatacg aaccgctaca
     9961 cgcagtaacc cctgacagga gtaaacaatg tcaaagcaac agatcggcgt cgtcggtatg
    10021 gcagtg
//

</pre>