LOCUS       KJ710509                8574 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain N 282 serotype O133:K-:H29 O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ710509
VERSION     KJ710509.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8574)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Baranzoni,G.M.,
            Swimley,M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: 50.1
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="N 282"
                     /serotype="O133:K-:H29"
                     /db_xref="taxon:562"
     misc_feature    1..8574
                     /note="O-antigen gene cluster"
     gene            126..1211
                     /gene="rmlB"
     CDS             126..1211
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="AIG62386.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFALHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            1211..2110
                     /gene="rmlD"
     CDS             1211..2110
                     /gene="rmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="AIG62388.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWIIHY
                     STDYVFPGTGEIPWQEADATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVSGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            2168..3046
                     /gene="rmlA"
     CDS             2168..3046
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase 1"
                     /protein_id="AIG62389.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNNVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVRVLAEPL
                     KKNAYGQYLLKMVKGY"
     gene            3051..3587
                     /gene="rmlC"
     CDS             3051..3587
                     /gene="rmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="AIG62390.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVE
                     FVQDNHSKSCKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFVYKTTNYYHPESDRGIIWNDPELQINWPVD
                     GELSLSKKDMKQPKFNVI"
     gene            3627..4868
                     /gene="wzx"
     CDS             3627..4868
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AIG62391.1"
                     /translation="MNKILKDIPIKSILHLFIVRGSIYLFPFITLPIITRNLGVAHFG
                     ILSLFLAAQQYFVMLVEYGFTLTGARDIVRCDNKQDEMKIINEIILCRLLIFLFCTAI
                     IIGAYAFFTEHGYISCYLILWSTVFCSVFNQTHFFIGKEKTGFIVISTCITRIVSIVI
                     VVLYVKDNRDINIALLAYSINTALPNILSTFYLNLYLKYKVKIIFCNNEIIDRFKSGY
                     DIFISNVFTSIYSTLTLMYLGTAKGSIETGYYSSADKLKAAAQGILFPVAQAFFPRVS
                     KECGISFYLLWRKSTTILVCFAVAIVASLFIFSNLIYILLLGEQYLSGINIYYILTLS
                     IIPISFGISYAQNLYLTQGQTKLLRKIYFAVSVLHLFHMPILVHFLGAIGAAISVLIT
                     ESFASLLMFIFRKKCYTWSQA"
     CDS             4844..5746
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="AIG62392.1"
                     /translation="MLHMESSLKIFAVIVTYNPDMDSFIRNLSAILGQVDTIIIVDNS
                     NEENYQISLKNHVDNISNVILIQPNENLGIAYAQNIGFMQSLKLNADFVITFDQDSSV
                     DSDLIEVLYSEYTKIKDNYNYKIACIGPSVINERNNARYEKYFKNSTQLNESVYSVKS
                     IISSGSLYSMDVFSAIGFNKAEWFIDSIDIEWCYRARYLGYHVLMTTNVAMKHNLGAR
                     DVKLPGGKSINIGSPFRLYYVFRNWIFSLREPQFDIKYKMKILLMMPIKFIIFSTVSP
                     RVSRFRFMLKGIKDGLLKKHSFIK"
     gene            5752..6858
                     /gene="pglA"
     CDS             5752..6858
                     /gene="pglA"
                     /codon_start=1
                     /transl_table=11
                     /product="N,
                     N'-diacetylbacillosaminyl-diphospho-undecaprenol
                     alpha-1,3-N-acetylgalactosaminyltransferase"
                     /protein_id="AIG62393.1"
                     /translation="MKISITANTSWYLYNFRRNTISALLNAGYEVIALSPDTEYKSKL
                     ETLGCKCFTIKMQRWGKNPLQDLQTILSFYTIFKKEKVNITLNFTPKNNIYATLAANL
                     LNIKVINNIAGLGVAFIQNTLFSKLVSYLYKVSQRKADYIFFQNEEDKELFLKLKIVS
                     ENKCERIPGSGVDLKRFEYSPIENTKNIRFLLVARLIAEKGIYIYVEAARAIKKLFPD
                     TEFYLLGPLDTCSTSGIKKYELDQWQKEKIITYLGFTDSVELELIKAHCVVLPSYYRE
                     GVPKSLLEAAAIGRPIITTDNIGCREVVDHGINGFLCEPGSIISLADNMERFLTLSSM
                     EKEIMGRNSRAKIQKDFDEKIVIEKYFSVVNSLK"
     gene            7172..8329
                     /gene="wzy"
     CDS             7172..8329
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="AIG62394.1"
                     /translation="MAYLFISLLFVLLFAEIKITQRKFSPTIVLITLWIGVLIVTQIY
                     SSALWQLSVETMWWVFGGVFAFFIGGFWGKLFFKIYGFKNISLQSNGNTLLFAIPFYI
                     LMFFFYYFMVRAGANEDQNWYVGIRKIINYGEPDLFFIMFGYIYYLIYPMLYLEAARY
                     YSSKSFNKKDKYNYHMQLIMCILYAVMSTAKLKLLLVIVPVFFIRSYFKPTSTKLIIV
                     VGTMFLGVFFLSLILLNKIAGDIGVFKIIITSIGNYTFFNLYAFDSLHFKEFLKIKCI
                     DNSNSCSLLPFFEYGDFRTNIYTIMYSFIEYGFFSYSLFLFMVGFIHNAADQIAKKTS
                     NIYAIILSSVLYVPLVFQIMDNQYTASKYLIYILMFSYVVYFTNVRRLRVM"
ORIGIN      
        1 taggcatgca tgcagtgctc tggtagctgt aaagccaggg gcggtagcgt gcattaatac
       61 ctctattaat caaactgaga gccgcttatt tcacagcatg ctctgaagta atatggaata
      121 ataaagtgaa gatacttgtt actggtggcg caggatttat tggttctgct gtagttcgtc
      181 acattataaa taatactcag gatagtgttg ttaatgtcga taaattaacg tacgccggaa
      241 acctggaatc acttgctgat gtttctgatt ctgaacgcta tgtttttgaa catgcggata
      301 tttgcgatgc agctgcaatg gcacggattt ttgctctgca tcagccggat gcagtgatgc
      361 acctggctgc tgaaagccat gtggatcgtt caattacagg ccctgcggca tttattgaaa
      421 ccaatattgt tggtacttat gtccttttgg aagccgctcg caattactgg tctgctcttg
      481 atggcgacaa gaaaaatagc ttccgttttc atcatatttc tactgacgaa gtttatggcg
      541 atctgcctca tccagatgaa gtaaataata cagaagaatt acccttattt actgagacga
      601 cagcttacgc gccaagcagc ccttattccg catccaaagc atccagcgat catttagtcc
      661 gtgcgtggaa acgtacctat ggtttaccga ccattgtgac taattgttcg aataactacg
      721 gtccttatca ctttccggaa aaattgattc cactagtaat tcttaatgct ctggaaggta
      781 aggcattacc tatttatggc aaaggggatc aaattcgcga ctggctgtat gttgaagatc
      841 atgcacgtgc gttatatacc gtcgtaaccg aaggtaaagc gggtgaaact tataacattg
      901 gtggacacaa cgaaaagaaa aacatcgatg tagtgctcac tatttgtgat ttgttggatg
      961 agattgtacc gaaagagaaa tcttaccgcg agcaaattac ttatgttgcc gatcgtccgg
     1021 gacacgatcg ccgttatgcc attgatgctg agaagattgg tcgcgaattg ggatggaaac
     1081 cacaggaaac gtttgagagc gggattcgga agacagtgga atggtacctg tccaatacaa
     1141 aatgggttga taatgtgaaa agtggtgcct atcaatcgtg gattgaacag aactatgagg
     1201 gccgccagta atgaatatcc tccttttcgg caaaacaggg caggtaggtt gggaactaca
     1261 gcgtgctctg gcacccctgg gtaatttgat tgctcttgat gttcactcca ctgattactg
     1321 tggtgatttt agtaatcctg aaggtgtagc tgaaaccgta agaagcattc ggcctgatat
     1381 tattgtcaac gcagccgctc acaccgcagt agacaaagca gaatcagaac cggagtttgc
     1441 acaattactt aacgcgacga gtgtcgaagc gatcgcgaaa gcagccaatg aagtcggcgc
     1501 ctggattatt cactactcta ctgactacgt atttccgggg accggtgaaa taccatggca
     1561 ggaggcggat gcaaccgcac cgctaaatgt ttacggtgaa accaagttag ctggagaaaa
     1621 agcattacaa gagcattgtg ccaagcacct aattttccgt accagctggg tctatgcagg
     1681 taaaggaaat aacttcgcca aaacgatgtt gcgtctggca aaagagcgtg aagaattagc
     1741 cgttattaat gatcagtttg gtgcgccaac aggtgctgaa ctgctggctg attgtacggc
     1801 acatgcaatt cgtgtggcac tgaataaacc agaagtctca ggcttgtacc atctggtagc
     1861 cagtggtacc acaacctggc acgattatgc tgcgctggtt tttgaagagg cacgaaaagc
     1921 aggtattccc cttgcactca acaagctcaa cgcagtacca acaacagcct atcctacacc
     1981 agctcgtcgc ccacataact ctcgccttaa tacagaaaaa tttcagcaga actttgcgct
     2041 tgtcttgcct gactggcagg ttggtgtgaa acgaatgctc aacgaattat ttacgactac
     2101 agcaatttaa tagtttttgc atcttgttcg tgatggtgga gcaagatgaa ttaaaaggaa
     2161 tgatgaaatg aaaacgcgta aaggtattat tttagcgggt ggttctggta ctcgtcttta
     2221 tcctgtaact atggctgtca gtaaacagct gttaccgatt tatgataaac cgatgatcta
     2281 ttatccgctc tctactctga tgttagcggg tattcgcgat attctgatta tcagtacgcc
     2341 acaggatact cctcgttttc aacaactgct gggtgacggg agccagtggg ggttaaatct
     2401 tcagtacaaa gtgcaaccga gtccagatgg tcttgctcag gcatttatca tcggtgaaga
     2461 gtttatcggt ggtgatgatt gtgctttggt tctaggtgat aatatctttt acggtcacga
     2521 tctgccgaag ttaatggacg tagctgttaa caaagaaagt ggtgcaacgg tatttgccta
     2581 tcacgttaat gatcctgaac gctacggtgt cgttgagttt gataaaaacg gtacggcaat
     2641 aagcctggaa gaaaaaccgc tacaaccaaa aagtaattat gcggtaaccg ggctttattt
     2701 ctatgataac aacgttgtcg aaatggcgaa aaaccttaag ccttctgccc gtggtgaact
     2761 ggaaattacc gatattaacc gtatttatat ggaacagggg cgtttatccg ttgccatgat
     2821 ggggcgtggt tatgcatggc tggatacggg gacacatcag agtcttattg aagcaagcaa
     2881 cttcatagca acaattgaag agcgtcaggg cttgaaagtt tcctgtccgg aagaaattgc
     2941 ttaccgtaaa ggatttattg atgctgagca ggttagggta ttagccgagc cacttaagaa
     3001 aaatgcttat ggtcaatatt tgctgaaaat ggttaaaggt tattaataaa atgaatgtaa
     3061 ttaaaacaga aattccagat gtactgattt ttgaaccgaa agtttttggt gatgagcgtg
     3121 gtttcttttt tgagagcttt aaccaaaagg tttttgagga agctgtaggc cgcaaagttg
     3181 aatttgtcca ggataaccat tcgaagtctt gtaaaggtgt tttacgcggg ctgcattatc
     3241 agttagaacc ttatgcgcaa gggaaattgg tacgttgcgt tgttggtgag gtttttgatg
     3301 ttgcggttga tattcgtaaa tcgtcaccaa cttttggaaa atgggttggg gtgaatttat
     3361 ctgctgagaa taagcgccag ttgtggatac ctgaaggatt tgcgcatggg tttttggtgc
     3421 tgagtgaaac tgctgagttt gtttataaga cgacgaatta ttatcatcca gagagtgata
     3481 gagggattat ttggaatgat cctgaactac aaattaattg gccagtagat ggagagttaa
     3541 gtctttctaa aaaagatatg aaacaaccta aatttaatgt tatctaatat aatgccccct
     3601 taagtgaatt aaggggggaa tataatatga ataaaattct aaaagatata ccaattaagt
     3661 ctatattgca tctgtttatt gttcgaggga gtatctattt attccctttt attacattgc
     3721 cgatcataac ccgcaattta ggggttgctc actttggcat attatcctta tttcttgctg
     3781 cgcaacaata ttttgttatg cttgttgaat atggcttcac attaactgga gcaagagata
     3841 ttgtcaggtg tgataataaa caagatgaaa tgaaaattat caatgaaata atactgtgca
     3901 ggctactaat atttctattt tgcacagcga ttatcattgg tgcgtatgct ttttttacag
     3961 aacatggtta tatctcctgt tatttaattt tatggagcac ggttttttgc tctgtattta
     4021 accaaacaca tttttttatt ggaaaagaaa agacaggttt tattgtaata tccacatgta
     4081 ttactcgcat agtttcgata gtaattgtgg ttctttatgt taaagacaat agagatatca
     4141 atattgctct tttggcatat tcaattaata cagcattacc aaacatcttg tcaactttct
     4201 atcttaattt gtatctcaag tataaagtaa aaattatttt ttgtaataac gaaataatag
     4261 accgttttaa aagtgggtat gatatattca tttcaaatgt tttcacaagt atttatagta
     4321 cattaacgtt aatgtattta gggactgcaa aaggatctat agaaacaggc tattattcaa
     4381 gtgctgacaa attaaaggct gctgctcaag gtattttatt tcctgttgcg caggcatttt
     4441 tccctagagt ttcgaaagaa tgtggcatca gcttttattt gttatggcga aaatcgacga
     4501 ctatattagt ttgtttcgca gtagcaatcg tagcaagcct attcatattc agtaatctta
     4561 tctatatatt gttactcgga gagcaatatt tatcaggtat taatatttat tatatattaa
     4621 ctttatctat aatccctatt tcatttggga tctcttatgc ccaaaaccta tacttgacgc
     4681 aagggcagac aaaactactt cggaaaatat attttgccgt atctgtacta catttgttcc
     4741 atatgccaat acttgtgcat tttttgggcg caataggtgc cgcaatctcg gttttaatca
     4801 ctgaatcatt tgcgtctcta ctaatgttta tctttaggaa aaaatgttac acatggagtc
     4861 aagcttgaaa atatttgcag taatagtaac gtataatcca gatatggatt ctttcattag
     4921 aaacctatca gcaattttag ggcaagttga tacaattatt attgttgata acagtaatga
     4981 agaaaactat cagatttctt taaaaaatca tgtagataat atcagcaatg tgattttaat
     5041 tcagccaaat gaaaatctcg gtattgcata tgcacaaaat ataggattta tgcaatcatt
     5101 aaaattaaat gccgattttg tgatcacatt tgaccaagac tcatctgttg atagtgatct
     5161 aattgaggta ctgtattcag aatatacaaa aataaaagat aattataatt ataaaatcgc
     5221 ttgtataggg ccctcagtaa ttaatgaaag gaacaacgca agatatgaaa aatacttcaa
     5281 aaatagcaca caattaaatg aatcagtata tagtgtaaaa tcaattatat cgtcgggttc
     5341 tttgtatagt atggatgttt tttctgcaat tggctttaat aaagcagagt ggtttattga
     5401 ttctattgat atagagtggt gctatcgtgc aagatatctt ggctatcatg ttttaatgac
     5461 aaccaatgta gcaatgaaac ataatttggg ggcaagagat gttaaattac caggagggaa
     5521 gtctattaat ataggttctc cttttagact ttattatgta tttaggaact ggattttttc
     5581 tcttcgagag cctcagtttg atattaaata taaaatgaaa atattattaa tgatgccgat
     5641 taaatttatt atattttcta ccgtttcacc ccgtgtatct cgatttcgat ttatgttaaa
     5701 ggggatcaaa gatggacttt tgaaaaaaca ttcatttata aaatagaaga catgaaaatt
     5761 tcaataacag caaacactag ttggtactta tataacttta gacggaatac tatttctgca
     5821 ttattaaatg caggatatga agtcatagct ttatctccag atacagagta taaatcgaag
     5881 ctagagactc tcggttgtaa atgtttcaca ataaagatgc aaagatgggg gaagaacccg
     5941 ttacaagatt tgcaaacaat tcttagcttt tatacaatct ttaaaaaaga aaaagtaaat
     6001 atcacactta attttacacc taaaaataat atttatgcaa cattagcagc taatttgcta
     6061 aatattaaag ttattaataa tattgccgga ttgggggttg cgtttataca aaatactttg
     6121 ttttcaaaat tggtatctta tctctataag gttagtcaaa gaaaggctga ttatattttc
     6181 tttcaaaatg aagaagacaa agagttgttt ttaaaactta agattgtatc agaaaataaa
     6241 tgtgagagga ttccaggttc tggtgtggat ttaaagcgat ttgaatattc gcccatagaa
     6301 aatacaaaaa acattcgatt cttattagtt gccagattga tagctgagaa aggtatttat
     6361 atctatgttg aagcagcaag agctattaaa aaactattcc cagatacaga gttttatttg
     6421 ctaggcccac tggatacctg tagtacttca ggaataaaaa aatatgaact ggatcaatgg
     6481 caaaaagaaa aaataataac ttatcttggg tttactgatt cagttgagct tgagctgata
     6541 aaagcacatt gcgttgtatt accatcttac tacagagaag gagtaccaaa atcattgtta
     6601 gaggccgcag caattggtag acctatcatt acaaccgata atataggttg tagagaggtt
     6661 gtggatcatg gtattaatgg attcctttgc gagcctggct caataattag tcttgcagat
     6721 aatatggaaa gatttttaac tttaagttca atggaaaaag aaataatggg tagaaacagt
     6781 agagctaaaa ttcaaaaaga ctttgatgag aaaattgtaa ttgaaaaata tttttctgtt
     6841 gtaaattcgt taaaatgaaa tgcttttata aatacattta tactctaaat aattcgattt
     6901 gcaggaaaac gatgaatccc taggagcttg aattccgaaa gttactagag ggaaggaact
     6961 cggtcgtagc acatacaact taaagtatga cgagtatata agaaattgta gctaatatct
     7021 atagtaaatt tgagaatata ttacaactta atcagtaaat ttaattattt gtttaagatt
     7081 ttcatattct gtacatgtgg ttggatagaa tatattatga tcatgtgttc tattttaaaa
     7141 ttaaatctta cttattcctc ggatagtaga aatggcttat ttatttattt cgttattatt
     7201 tgttttgcta ttcgctgaaa taaaaataac tcaaaggaag ttttctccga ctattgtatt
     7261 aataacatta tggattggtg ttttaatcgt aacccaaata tatagttctg cgttatggca
     7321 actgtcagtt gaaaccatgt ggtgggtttt tggtggggtg tttgcttttt ttataggtgg
     7381 attttggggg aagttatttt ttaaaatata tgggtttaaa aatatctctc tacaaagtaa
     7441 tggcaacaca cttttatttg ctattccttt ttatatttta atgttctttt tttattattt
     7501 tatggtcaga gcaggagcta atgaagatca aaactggtat gttggcatac gaaagataat
     7561 aaactatggt gagcctgatt tgttttttat aatgtttggg tatatttatt acttgatcta
     7621 cccaatgcta tatttagaag ctgcgaggta ttactcttca aagagtttca acaagaagga
     7681 taaatataat tatcatatgc aattaattat gtgcatatta tatgctgtaa tgtcaacggc
     7741 aaagcttaag cttttgctag tgattgttcc tgtgttcttt atacggagtt attttaaacc
     7801 aacaagtacg aaactaataa tagttgttgg cacgatgttt cttggggttt ttttcttatc
     7861 acttatttta cttaataaaa tagctggcga tataggtgtt ttcaaaataa ttataacatc
     7921 aataggtaac tatacatttt tcaatctata tgctttcgat tcacttcatt ttaaagagtt
     7981 tttaaaaatt aagtgtattg ataactcgaa tagctgttcc ttattaccgt ttttcgaata
     8041 tggtgatttt agaacaaata tatatactat aatgtattca tttattgaat atggtttctt
     8101 tagttatagc ctgttccttt ttatggttgg atttattcat aatgcggcag atcaaatagc
     8161 aaaaaaaaca tcaaatatat atgcaattat acttagctca gtgctttacg ttcctttggt
     8221 attccaaata atggataatc aatatacggc atcaaagtat ttgatatata tattgatgtt
     8281 ttcttatgtt gtatatttca ctaatgtgag acgtttgcga gtgatgtaat ttatttgatt
     8341 agttcaaaaa gcttgcgcgg gttataaatg aaattattat tgaaagatat tccattcatt
     8401 gaaatatttt ttaacattta cgtatcgata gattttctta ctcaagaata ccacttttca
     8461 agccgcggca actcgtggtg accaccccct gacaggagta aacaatgtca aagcaacaga
     8521 tcggcgtcgt cggtatggca gtaatggggc gcaaccttgc gctcaacatc gaaa
//